IF 3.3 3区生物学

Cell Adhesion & Migration Pub Date : 2025-12-01 Epub Date: 2025-03-30 DOI: 10.1080/19336918.2025.2484182

Hao Chen, Jine Wang, Bingzhu Zhao, Yahua Yang, Chongfa Yang, Zhijie Zhao, Xiaona Ding, Yang Li, Taojie Zhang, Zhaxi Yingpai, Shengdong Huo

{"title":"N-Acetylcysteine relieving hydrogen peroxide-induced damage in granulosa cells of sheep.","authors":"Hao Chen, Jine Wang, Bingzhu Zhao, Yahua Yang, Chongfa Yang, Zhijie Zhao, Xiaona Ding, Yang Li, Taojie Zhang, Zhaxi Yingpai, Shengdong Huo","doi":"10.1080/19336918.2025.2484182","DOIUrl":"10.1080/19336918.2025.2484182","url":null,"abstract":"Sheep ovarian granulosa cells (GCs) play a unique role in the ovary. Damage to GCs can affect the normal development of oocytes. The oxidative stress model was constructed by H2O2to study the biological changes. Specifically, pathological characteristic was assessed by immunohistochemistry (IHC), while signaling pathway was studied using western blot, quantitative RT-PCR, and immunofluorescence. Theresults showed that the oxidative damage model was successfully constructed by 200 μmol/LH2O2 for 12 h. NAC can protect the proliferation of GCs under H2O2-induced oxidative stress and reduce apoptosis. It can also promote the secretion of E2 and P4 by GCs and reduce the inflammatory response of GCs. NAC can enhance the expression of NRF2, PI3K and Akt. These findings suggest that NAC alleviates H2O2-induced oxidative stress injury through NRF2/PI3K/AKT signaling pathways. Provide ideas for studying the poor quality of mammalian oocytes.","PeriodicalId":9680,"journal":{"name":"Cell Adhesion & Migration","volume":"19 1","pages":"2484182"},"PeriodicalIF":3.3,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative use of gram-positive enhancer matrix particles and affinity peptides in a vaccine against Coxsackievirus B3. 革兰氏阳性增强子基质颗粒和亲和肽在柯萨奇病毒B3疫苗中的创新应用

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-05-14 DOI: 10.1080/21505594.2025.2481657

Shaoju Qian, Ruixue Li, Guanyu Chen, Yinghua Ma, Xuehan Zhang, Zhou Tang, Yihang Song, Zhishan Xu, Zihan Zhang, Yeqing He, Xingyi Zhang, Shuao Lu, Zishan Yang, Xiangfeng Song, Wenfa Yu, Lili Yu

{"title":"Innovative use of gram-positive enhancer matrix particles and affinity peptides in a vaccine against Coxsackievirus B3.","authors":"Shaoju Qian, Ruixue Li, Guanyu Chen, Yinghua Ma, Xuehan Zhang, Zhou Tang, Yihang Song, Zhishan Xu, Zihan Zhang, Yeqing He, Xingyi Zhang, Shuao Lu, Zishan Yang, Xiangfeng Song, Wenfa Yu, Lili Yu","doi":"10.1080/21505594.2025.2481657","DOIUrl":"10.1080/21505594.2025.2481657","url":null,"abstract":"Viral myocarditis (VM) is an inflammatory disease posing a serious threat to public health, with various viral pathogens contributing to its pathogenesis. Coxsackievirus B3 (CVB3) is the most frequently implicated causative agent and has been extensively studied because of its high prevalence and severity. No specific therapeutic interventions for VM exist, and vaccine development has encountered substantial challenges. Therefore, we aimed to develop a novel CVB3 mucosal vaccine as a preventive strategy against VM. Gram-positive enhancer matrice (GEM) particles serve as innovative mucosal vaccine adjuvants and antigen delivery systems that enhance antigen immunogenicity by facilitating effective mucosal immune responses. In this study, GEM particle display technology was used to develop two novel CVB3 vaccines: (1) a GEM particle-based vaccine displaying the CVB3 capsid protein VP1 via a PA anchor protein (GEM-PA-VP1), and (2) a GEM particle-based vaccine displaying VP1 via the FcSP peptide (GEM-Fc-VP1). Both GEM-PA-VP1 and GEM-Fc-VP1 vaacines significantly elevated levels of specific IgG, IgG1, IgG2a, sIgA and neutralizing antibodies in a mouse model, along with enhanced secretion of Th1- and Th2-associated cytokines, compared to controls. Notably, GEM-Fc-VP1 demonstrated superior immunogenicity compared with that of GEM-PA-VP1, evidenced by higher antibody titres and cytokine responses. In challenge protection experiments, both vaccines significantly improved survival rates, reduced myocardial enzyme levels, and decreased inflammatory cell infiltration in myocardial tissue, with GEM-Fc-VP1 exhibiting greater efficacy. These findings establish a foundation for the development of a safe and effective CVB3 candidate vaccine and provide novel insights into the potential of peptide-mediated subunit vaccine approaches.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2481657"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12080276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenome-wide association study of perceived discrimination in the Multi-Ethnic Study of Atherosclerosis (MESA). 多种族动脉粥样硬化研究（MESA）中感知歧视的全表观基因组关联研究。

IF 2.9 3区生物学

Epigenetics Pub Date : 2025-12-01 Epub Date: 2025-01-18 DOI: 10.1080/15592294.2024.2445447

Wei Zhao, Lisha Lin, Kristen M Kelly, Lauren A Opsasnick, Belinda L Needham, Yongmei Liu, Srijan Sen, Jennifer A Smith

{"title":"Epigenome-wide association study of perceived discrimination in the Multi-Ethnic Study of Atherosclerosis (MESA).","authors":"Wei Zhao, Lisha Lin, Kristen M Kelly, Lauren A Opsasnick, Belinda L Needham, Yongmei Liu, Srijan Sen, Jennifer A Smith","doi":"10.1080/15592294.2024.2445447","DOIUrl":"10.1080/15592294.2024.2445447","url":null,"abstract":"Perceived discrimination, recognized as a chronic psychosocial stressor, has adverse consequences on health. DNA methylation (DNAm) may be a potential mechanism by which stressors get embedded into the human body at the molecular level and subsequently affect health outcomes. However, relatively little is known about the effects of perceived discrimination on DNAm. To identify the DNAm sites across the epigenome that are associated with discrimination, we conducted epigenome-wide association analyses (EWAS) of three discrimination measures (everyday discrimination, race-related major discrimination, and non-race-related major discrimination) in 1,151 participants, including 565 non-Hispanic White, 221 African American, and 365 Hispanic individuals, from the Multi-Ethnic Study of Atherosclerosis (MESA). We conducted both race/ethnicity-stratified analyses as well as trans-ancestry meta-analyses. At false discovery rate of 10%, 7 CpGs and 4 differentially methylated regions (DMRs) containing 11 CpGs were associated with perceived discrimination exposures in at least one racial/ethnic group or in meta-analysis. Identified CpGs and/or nearby genes have been implicated in cellular development pathways, transcription factor binding, cancer and multiple autoimmune and/or inflammatory diseases. Of the identified CpGs (7 individual CpGs and 11 within DMRs), two CpGs and one CpG within a DMR were associated with expression of cis genes NDUFS5, AK1RIN1, NCF4 and ADSSL1. Our study demonstrated the potential influence of discrimination on DNAm and subsequent gene expression.","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"20 1","pages":"2445447"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is uric acid a true antioxidant? Identification of uric acid oxidation products and their biological effects. 尿酸是真正的抗氧化剂吗？尿酸氧化产物的鉴定及其生物学效应。

IF 5.2 2区生物学

Redox Report Pub Date : 2025-12-01 Epub Date: 2025-05-25 DOI: 10.1080/13510002.2025.2498105

Mikaela Peglow Pinz, Isadora Medeiros, Larissa Anastácio da Costa Carvalho, Flavia Carla Meotti

{"title":"Is uric acid a true antioxidant? Identification of uric acid oxidation products and their biological effects.","authors":"Mikaela Peglow Pinz, Isadora Medeiros, Larissa Anastácio da Costa Carvalho, Flavia Carla Meotti","doi":"10.1080/13510002.2025.2498105","DOIUrl":"10.1080/13510002.2025.2498105","url":null,"abstract":"Uric acid (UA), the final product of purine metabolism in humans, exhibits a dual role as an anti or pro-oxidant, depending on the microenvironment. The two-electron oxidation of UA by biological oxidants can neutralize such harmful molecules. Additionally, UA chelates metals and can activate adaptive response against oxidation. However, some products of the reaction between UA and oxidants are not inert and, therefore, do not confer the anticipated antioxidant protection. A direct pro-oxidant effect is favoured in the one-electron oxidation of UA by heme-peroxidases yielding free radical intermediates that can initiate or propagate a radical-chain reaction. Additionally, an indirect pro-oxidant effect has been proposed by eliciting the expression or activation of enzymes that catalyse oxidant production, e.g. NADPH oxidase (NOX). This review brings together fundamental concepts and the molecular mechanisms of the redox reactions involving UA. The signature metabolites from these reactions are discussed to give valuable insights on whether these intermediates are being formed and what role they may play in disease pathogenesis. It proposes that, through identifying specific products, it may be possible to elucidate whether a harmful or protective action is linked to downstream bioactivities.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"30 1","pages":"2498105"},"PeriodicalIF":5.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional signature of cardiac myocyte recovery in mice and human reveals persistent upregulation of epigenetic factors. 小鼠和人类心肌细胞恢复的转录特征揭示了表观遗传因子的持续上调。

IF 2.9 3区生物学

Epigenetics Pub Date : 2025-12-01 Epub Date: 2025-06-05 DOI: 10.1080/15592294.2025.2506625

Rebekka Roth, Margareta Häckh, Tilman Schnick, Carolin Rommel, Christoph Koentges, Heiko Bugger, Claudia Domisch, Michael R Bristow, Amrut V Ambardekar, Timothy A McKinsey, Ralf Gilsbach, Lutz Hein, Sebastian Preissl

{"title":"Transcriptional signature of cardiac myocyte recovery in mice and human reveals persistent upregulation of epigenetic factors.","authors":"Rebekka Roth, Margareta Häckh, Tilman Schnick, Carolin Rommel, Christoph Koentges, Heiko Bugger, Claudia Domisch, Michael R Bristow, Amrut V Ambardekar, Timothy A McKinsey, Ralf Gilsbach, Lutz Hein, Sebastian Preissl","doi":"10.1080/15592294.2025.2506625","DOIUrl":"https://doi.org/10.1080/15592294.2025.2506625","url":null,"abstract":"Fibrosis, cardiac remodelling, and inflammation are hallmarks of heart failure. To date, there is no available pharmacological cure for heart failure, but mechanical unloading by implantation of a left ventricular assist device (LVAD) can lead to improved cardiac function in a subset of patients. This study aimed to identify the transcriptional response of left ventricular (LV) cardiac myocytes to mechanical unloading in a mouse model of reversible LV pressure overload and in failing human hearts after LVAD implantation. We found that partial recovery of ventricular dysfunction, LV hypertrophy, and gene expression programmes occurred in mice under reversible transverse aortic constriction (rTAC). Gene expression analysis in cardiac myocytes identified a lasting repression of mitochondrial gene expression resulting in compromised fatty acid oxidation in the mouse model of reversible pressure overload and in human LV samples after LVAD therapy and a persistent upregulation of epigenetic and transcriptional regulators. These findings underpin that recovery from heart failure involves complex gene regulatory networks and that mitochondrial dysfunction remains a challenge even after mechanical unloading. Further studies are needed to investigate the functional role of these factors in reverse remodelling and recovery of failing hearts.","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"20 1","pages":"2506625"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the immune-related targetome of miR-155-5p by integrating time-resolved RNA patterns into miRNA target prediction. 通过将时间分辨RNA模式整合到miRNA靶标预测中，扩大miR-155-5p的免疫相关靶标组。

IF 3.6 3区生物学

RNA Biology Pub Date : 2025-12-01 Epub Date: 2025-01-11 DOI: 10.1080/15476286.2025.2449775

Martin Hart, Caroline Diener, Stefanie Rheinheimer, Tim Kehl, Andreas Keller, Hans-Peter Lenhof, Eckart Meese

引用次数: 0

Stage-specific modulation of Drosophila gene expression with muscle GAL4 promoters. 肌肉GAL4启动子对果蝇基因表达的阶段性调控。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2025-01-07 DOI: 10.1080/19336934.2024.2447617

Ziwei Zhao, Erika R Geisbrecht

引用次数: 0

Fungal chimera: A lethal mammalian fungus with invasion strategies of plant pathogens. 真菌嵌合体：一种具有植物病原体入侵策略的致命哺乳动物真菌。

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2024-12-26 DOI: 10.1080/21505594.2024.2439497

Carol Uphoff Meteyer, Justin G Boyles

引用次数: 0

EIciRNAs in focus: current understanding and future perspectives. 关注eicirna：当前的理解和未来的观点。

IF 3.6 3区生物学

RNA Biology Pub Date : 2025-12-01 Epub Date: 2024-12-23 DOI: 10.1080/15476286.2024.2443876

Yan Yang, Yinchun Zhong, Liang Chen

引用次数: 0

Disentangling how the brain is wired. 解开大脑是如何连接的。

IF 2.4 4区生物学

Fly Pub Date : 2025-12-01 Epub Date: 2024-12-31 DOI: 10.1080/19336934.2024.2440950

Simon G Sprecher

引用次数: 0

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