Journal of Molecular Histology最新文献

{"title":"Immunohistochemical expression of pancreatic melatonin receptor (MTNR1B) under experimental sleep disturbance in rats.","authors":"Salwa Hameed Affat, Huda Rashid Kamoona","doi":"10.1007/s10735-026-10817-z","DOIUrl":"https://doi.org/10.1007/s10735-026-10817-z","url":null,"abstract":"<p><p>Sleep amount and quality are important to maintain health, light exposure transported to the pineal gland via suprachiasmatic nucleus pineal pathway, and this affect melatonin synthesis. Melatonin exerts its action by receptors called MT1, MT2 that found in peripheral tissues including pancreas, which affect its endocrine function. In this study we investigate the effect of type of sleep disturbance on the histological structure and the expression of melatonin receptor (MTNR1B) in the pancreas. A sample of 45 adult healthy male rats had free access to water and feeding, divided into 3 groups (15 in each group): the control group had normal 24 h diurnal variation, group A subjected to interruption of sleep by light exposure for 2 h at three intervals, and group B subjected to a reduction in sleep duration by 7 h. This experiment continued for 14 days, animals were scarified by euthanasia by cervical dislocation, pancreatic tissue prepared for paraffin blocks and stained by H&E, and IHC for MTNR1B. Histomorhometric analysis done by image J (V 1.54), immunohistochemical assessment done by Aprioscope image analysis software 12.4.6.5003) mean total positivity of expression was selected, statistical analysis done by SSPS (25 V) and Tukeyꞌs test. Histological evaluation revealed changes in sleep disturbance groups including fat deposition, vascular dilatation, and apoptotic changes for group of cells within islets of Langerhans which showed significant reduction in their count in sleep disturbance groups compared to control, and non-significant change in their area across the groups, but significant reduction in area in sleep deprived group compared to sleep interruption. Immunohistochemical expression of MTNR1B showed it only expressed within the area of islets of Langerhans, with non-significant increase in expression in sleep disturbed groups, being more in sleep deprived group. This study showed that the pancreas is affected by sleep disturbance patterns, mainly its endocrine part (islets of Langerhans), with study expression of melatonin receptor within them. This may suggest a role of melatonin in maintaining pancreatic ꞵ cells.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"57 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into the neuroprotective effects of yangyin formula on cerebral ischemia-reperfusion injury via the IL-17A/MAPK/NF-κB signaling pathway","authors":"Kun Shi,&nbsp;Haofang Wan,&nbsp;Jin Han,&nbsp;Jiehong Yang,&nbsp;Haitong Wan","doi":"10.1007/s10735-026-10766-7","DOIUrl":"10.1007/s10735-026-10766-7","url":null,"abstract":"<p>Ischemic stroke is a leading cerebrovascular disorder frequently complicated by cerebral ischemia-reperfusion injury (CIRI), which aggravates neurological damage and worsens clinical outcomes. Yangyin Formula (YYF), a traditional Chinese medicine composed of <i>Rehmannia glutinosa</i>, <i>Dendrobium officinale</i>, and <i>Pueraria lobata</i>, has shown potential in mitigating CIRI. To elucidate its therapeutic mechanisms, we integrated network pharmacology, molecular docking, and molecular dynamics simulations, followed by in vivo validation in a rat model of middle cerebral artery occlusion/reperfusion (MCAO/R). A total of 61 bioactive compounds and 768 targets were identified, enriched in 136 signaling pathways. Computational analyses revealed strong binding affinities between key YYF components and CIRI-related targets. In vivo, YYF administration significantly improved neurological function, reduced infarct volume, and alleviated histopathological damage. Furthermore, YYF suppressed IL-17A, TNF-α, IL-1β, Caspase-3, p38 MAPK, and NF-κB p65 expression in brain and serum, with effects comparable to IL-17A inhibition. These findings suggest that YYF exerts neuroprotective effects against CIRI by modulating the IL-17A/p38 MAPK/NF-κB p65 signaling pathway, highlighting its potential as a therapeutic strategy for ischemic stroke.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"57 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aquaporin-9 expression as an independent adverse prognostic marker in diffuse large B-cell lymphoma: an immunohistochemical tissue microarray study","authors":"Mayada Saad Farrag,&nbsp;Mohamed S. Hemada,&nbsp;Salma Elashwah,&nbsp;Madonna I. William,&nbsp;Shaimaa El-Ashwah,&nbsp;Eman Mohamad Ibrahim","doi":"10.1007/s10735-026-10821-3","DOIUrl":"10.1007/s10735-026-10821-3","url":null,"abstract":"<div><p>Aquaporins (AQPs) are membrane channel proteins implicated in tumor biology, but their clinicopathological and prognostic relevance in diffuse large B-cell lymphoma (DLBCL) remains insufficiently characterized. This study evaluated the immunohistochemical expression of AQP1, AQP2, AQP8, and AQP9 and their associations with clinicopathological features and survival outcomes in DLBCL. In this retrospective cohort study, formalin-fixed, paraffin-embedded tissue samples from 164 DLBCL cases diagnosed at Mansoura University Oncology Center between 2011 and 2022 were analyzed using tissue microarray-based immunohistochemistry. Associations between marker expression and clinicopathological variables were assessed using appropriate comparative tests. Overall survival (OS) and disease-free survival (DFS) were analyzed using Kaplan–Meier methods and Cox proportional hazards regression. A sensitivity Cox analysis was additionally performed to assess whether the association of AQP9 with overall survival persisted after adjustment for rituximab exposure. Positivity for AQP1, AQP2, AQP8, and AQP9 was detected in 9.8% (16/164), 1.8% (3/164), 0.6% (1/164), and 11.6% (19/164) of cases, respectively. AQP1 positivity was associated with higher International Prognostic Index (IPI) risk category (<i>P</i> = 0.007) and lower hemoglobin levels (<i>P</i> = 0.012), but not with significant differences in survival. AQP9 expression was significantly associated with inferior OS, with a median OS of 20.7 months in AQP9-positive cases versus 78.6 months in AQP9-negative cases (log-rank <i>P</i> = 0.002). In multivariable analysis, AQP9 remained an independent adverse predictor of mortality (HR 2.44, 95% CI 1.35–4.40; <i>P</i> = 0.003). This association remained significant in a sensitivity model additionally adjusting for rituximab exposure (HR 2.26, 95% CI 1.25–4.09; <i>P</i> = 0.007). None of the evaluated markers significantly predicted DFS. AQP9 expression identifies a subgroup of DLBCL patients with significantly poorer overall survival and may provide prognostic information beyond conventional clinical risk stratification. In contrast, AQP1 was associated with adverse baseline features but had no significant impact on survival, while AQP2 and AQP8 were rarely expressed. Larger multi-center studies are warranted to validate the prognostic role of AQP9 in DLBCL.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"57 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xerophilusin B attenuates wear particle: associated osteolysis by inhibiting NF-κB signaling and restoring osteoblast–osteoclast coupling","authors":"Hui Shi,&nbsp;Qian Meng,&nbsp;Tao Meng,&nbsp;Changling Du,&nbsp;Chuang Xu,&nbsp;Heng Cao,&nbsp;Kefan Zhang,&nbsp;Jian Li","doi":"10.1007/s10735-026-10820-4","DOIUrl":"10.1007/s10735-026-10820-4","url":null,"abstract":"<div><h3>Background</h3><p>Periprosthetic osteolysis after total joint arthroplasty is the leading cause of aseptic loosening. Bone metabolic imbalance driven by aberrant NF-κB activation mediated by titanium wear particles is a central pathogenic mechanism. Available therapies have clear limitations, and the role and mechanism of Xerophilusin B (XB) in this setting have not been clarified. To evaluate the regulatory effects of XB on titanium wear particle–induced osteolysis and to define the key mechanism involved, with the aim of identifying a potential candidate for preventing and treating periprosthetic osteolysis. RAW264.7 osteoclast precursors and MC3T3-E1 osteoblasts were treated with XB at 0, 5, 10, or 20 µmol/L. Titanium wear particles were used to establish an in <i>vitro</i> model of bone metabolic imbalance. Cell viability and proliferation were assessed with CCK-8 and EdU staining. Osteoclast differentiation and function were evaluated by TRAP staining and a bone resorption pit assay. Osteoblast differentiation and mineralization were examined using ALP staining and Alizarin Red S staining. Western blotting was used to quantify bone-metabolism-related proteins and NF-κB pathway proteins. In vivo, 48 C57BL/6 mice were randomly assigned to the control group, Wear group, Intervention group, and Combination group (NF-κB activator). A calvarial osteolysis model was established. After 4 weeks of continuous intraperitoneal administration, histological staining, immunohistochemistry, ELISA, and Western blotting were performed to verify in vivo effects and mechanisms. XB at 10 µmol/L showed no detectable cytotoxicity and was used as the working concentration. Under titanium wear particle stimulation, XB reduced the number of TRAP-positive multinucleated osteoclasts by 56.79%, indicating marked inhibition of osteoclast differentiation and resorptive activity. In MC3T3-E1 cells, the ALP-positive staining area recovered to 87.82%, showing that XB counteracted particle-mediated suppression of osteogenic differentiation and mineralization. At the signaling level, XB blocked abnormal phosphorylation of IKKα/β and p65, restrained NF-κB activation, and modulated the expression of bone-metabolism-related proteins including TRAP, CTSK, Runx2, and OCN. In vivo, XB alleviated calvarial osteolysis-associated pathological injury, adjusted serum markers of bone resorption and formation, and showed no apparent hepatorenal toxicity at the experimental dose. An NF-κB pathway activator largely attenuated XB’s osteoprotective effect. By inhibiting NF-κB signaling, XB bidirectionally regulates osteoclast–osteoblast function and bone metabolic homeostasis, effectively mitigating titanium wear particle–induced calvarial osteolysis.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"57 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lidocaine alleviates diabetic neuropathic pain by inhibiting spinal astrocyte activation through modulation of the CXCL13/CXCR5 axis","authors":"QiaoZhen Liu,&nbsp;Hua Zhang","doi":"10.1007/s10735-026-10806-2","DOIUrl":"10.1007/s10735-026-10806-2","url":null,"abstract":"<div><p>This study aimed to investigate the analgesic effects of lidocaine (Lido) on diabetic neuropathic pain (DNP), a common complication of diabetes, and to elucidate the underlying mechanism. A DNP mouse model was established by a single streptozotocin injection at 200 mg/kg. Two weeks later, mice received intraperitoneal infusion with 2% Lido for 7 consecutive days. The analgesic effect of Lido was assessed by pain behavioral tests. Astrocyte activation, pro-inflammatory cytokine levels, and CXCL13 and CXCR5 expression were examined in mechanistic studies. Lido significantly improved mechanical allodynia and thermal hyperalgesia in DNP mice. Lido reduced spinal astrocyte activation and pro-inflammatory cytokine levels in DNP mice. It also inhibited CXCL13 and CXCR5 expression, with their upregulation diminishing Lido’s analgesic effects. Lido alleviates DNP by inhibiting spinal astrocyte activation via modulation of the CXCL13/CXCR5 axis.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"57 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-organ histological assessment of Agrococcus sp. RKDAS_1 reveals tissue-level biocompatibility in Oreochromis niloticus","authors":"Venkatesan Karthick,&nbsp;Arun Venkatesh,&nbsp;Singamoorthy Amalraj,&nbsp;Sulochana Sonti,&nbsp;Rajkumar Thamarai,&nbsp;Rajkumar Prabhakaran","doi":"10.1007/s10735-026-10819-x","DOIUrl":"10.1007/s10735-026-10819-x","url":null,"abstract":"<div><p>A histological safety profile is essential for non-antibiotic microbial interventions in aquaculture. This study assessed the multi-organ histological biocompatibility of a novel actinobacterial strain, <i>Agrococcus</i> sp. RKDAS_1, in Nile tilapia (<i>Oreochromis niloticus</i>) under controlled conditions. Juvenile tilapia were fed diets with varying concentrations of RKDAS_1 (10⁵, 10⁷, and 10⁹ CFU g^{− 1} feed) for 60 days. Tissues from the gill, intestine, liver, and heart were analysed through standard histological methods, employing semiquantitative scoring and intestinal morphometry. Across all examined organs, RKDAS_1 supplementation did not induce inflammatory responses, degenerative lesions, or structural disruptions, which are indicative of tissue-level toxicity. Gill architecture was intact, with normal hepatocyte arrangements and no necrosis or fibrosis, while cardiac tissues showed a normal structure. Intestinal morphology maintained epithelial integrity, displaying dose-related variations in villus height and goblet cell density. Intermediate-dose live hepatic sections showed reduced cytoplasmic vacuolation compared to controls, though the difference was not statistically significant. The semi-quantitative histopathological evaluation showed that the tissue structure remained intact across different treatments. The results collectively suggest that <i>Agrococcus</i> sp. RKDAS_1 did not cause any noticeable histopathological damage, indicating enhanced biocompatibility. The findings suggest that <i>Agrococcus</i> sp. RKDAS_1 is compatible with biological tissues without causing significant damage. However, these conclusions are restricted to structural analysis and do not ensure functional improvements or overall safety beyond tissue examination. The findings provide a critical starting point for future research, which will delve into molecular, immunological, and long-term exposure studies. These investigations aim to evaluate the biotherapeutic potential of RKDAS_1 thoroughly.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"57 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and antioxidant assessment of the gastroprotective effect of polyphenolic extract from Corchorus olitorius in an ethanol-induced gastric ulcer model","authors":"Ahlam Kherbouche,&nbsp;Meriem Ghalem,&nbsp;Fatima-zohra Boukli Hacene,&nbsp;Nabila Yelles,&nbsp;Reda Ali Bettioui,&nbsp;Abdelouahab Mebarki,&nbsp;Hafida Merzouk,&nbsp;Mouna-Messaouda Kaci,&nbsp;Smail Meziane,&nbsp;Said Ghalem","doi":"10.1007/s10735-026-10809-z","DOIUrl":"10.1007/s10735-026-10809-z","url":null,"abstract":"<div>\u0000 \u0000 <p>Gastric ulcers remain a global health concern, with common treatments such as proton pump inhibitors often causing adverse effects. Polyphenol-rich extracts represent promising alternatives due to their antioxidant and gastroprotective properties. This study investigated the antiulcerogenic and antioxidant potential of Polyphenolic Extract from <i>Corchorus olitorius</i> (PECO) leaves in an ethanol-induced rat gastric ulcer model. Phenolic, flavonoid, and tannin contents were quantified by colorimetric assays, while HPLC-UV/Vis identified major bioactive compounds. Antioxidant, anti-inflammatory, and hemolytic (cytotoxicity) activity on human erythrocytes were assessed in vitro. Antiulcerogenic activity was evaluated in Wistar rats through preventive and curative protocols with polyphenolic extract doses of 100 and 400 mg/kg, compared to omeprazole. Gastric pH, ulcer index, antioxidant status (PAOT^®/POT^®), polyphenol levels, and histopathology were analyzed. PECO contained high levels of phenols (237.84 µg GAE/mg) and flavonoids (297.43 µg CE/mg). HPLC identified 13 compounds, including salicylic acid, caffeine, rutin and catechin. In vitro, PECO displayed strong antioxidant activity (1032.18 PAOT score/g), significant anti-inflammatory effects (IC₅₀ = 85.36 µg/mL). PECO showed low hemolytic activity on human erythrocytes, suggesting limited cytotoxicity in this model. In vivo, PECO significantly restored gastric pH, reduced ulceration up to 92.77% in preventive protocols and 90.44% in curative protocols, and improved gastric antioxidant and phenolic contents Histological analysis confirmed mucosal protection and regeneration, with comparable effects to omeprazole. The polyphenol-rich extract of <i>Corchorus olitorius</i> exhibited strong antioxidant, anti-inflammatory, and gastroprotective activities. At (400 mg/kg), it significantly reduced gastric lesions and restored antioxidant balance, similar to omeprazole, suggesting its potential as a natural therapeutic candidate for gastric ulcer prevention and management.</p>\u0000 </div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"57 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cherry plum powder alleviates excessive proliferation and inflammation of keratinocytes in psoriasis by reducing N6 methyladenosine-modified S100A4","authors":"Huiqin Wang,&nbsp;Yuan Ding,&nbsp;Shirong Yu,&nbsp;Weidong Wu","doi":"10.1007/s10735-026-10815-1","DOIUrl":"10.1007/s10735-026-10815-1","url":null,"abstract":"<div>\u0000 \u0000 <p>Cherry plum powder (CPP) is a pure natural plant extract with antioxidant and anti-inflammatory effects, and its role in psoriasis has not yet been elucidated. In this study, Balb/c mice were treated by imiquimod (IMQ) for a consecutive 7 days to establish psoriasis models, and then administrated with CPP by gavage for 2 weeks. CPP treatment obviously alleviated IMQ-induced psoriasis-like dermatitis, epidermal thickening and inflammatory cytokines secretion in mice. Through in vitro keratinocytes models, CPP was also found to suppress tumor necrosis factor tumor necrosis factor-alpha (TNF-α)-induced excessive proliferation and inflammatory cytokines production in HaCaT cells. Besides, CPP significantly reduced the expression of soluble protein 100 protein A4 (S100A4) and the phosphorylation of phosphatidylinositol 3-kinase (PI3K) and serine/threonine kinase (AKT) in psoriasis models in vivo and in vitro. In mechanism, methyltransferase-like protein 14 (METTL14)-mediated N6 methyladenosine (m6A) methylation stabilized and upregulated S100A4 mRNA, and then activated the downstream PI3K/AKT signaling, which was reversed by CPP treatment. Overexpression of METTL14 or S100A4 counteracted the therapeutic effects of CPP on TNF-α-induced HaCaT cells, which were then restored by a PI3K inhibitor. To conclude, CPP alleviates excessive proliferation and inflammation of keratinocyte to improve psoriasis through suppressing METTL14-mediated m6A modification of S100A4 mRNA and inactivating the downstream PI3K/AKT signaling pathway.</p>\u0000 </div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"57 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrogen promotes osteogenesis via YAP1-driven activation of ERα orchestrated through TEAD1 binding to the ERα promoter","authors":"Ruiying Han,&nbsp;Yikai He,&nbsp;Fushuang Liu,&nbsp;Tianyi Wang,&nbsp;Qi Wang,&nbsp;Wanxi Chen,&nbsp;Ding Bai,&nbsp;Yongwen Guo","doi":"10.1007/s10735-026-10810-6","DOIUrl":"10.1007/s10735-026-10810-6","url":null,"abstract":"<div>\u0000 \u0000 <p>Bone remodeling is a dynamic process that is essential for maintaining skeletal integrity and homeostasis. Although crosstalk between Yes-associated protein 1 (YAP1) and estrogen receptor alpha (ERα) has been implicated in bone biology, the mechanism by which these pathways cooperate during osteogenesis remains incompletely defined. Here, primary mouse bone marrow stromal cells (BMSCs) were isolated and characterized, and then stimulated with 17β-estradiol (E2). E2 significantly enhanced osteogenic differentiation and increased the expression of ERα, YAP1, and osteogenic markers. Genetic silencing or pharmacological inhibition of YAP1 attenuated E2-induced osteogenesis and reduced ERα expression, indicating that YAP1 is required for the osteogenic response to E2. Conventional chromatin immunoprecipitation (ChIP)-PCR followed by agarose gel electrophoresis provided qualitative evidence that TEAD1 associates with the ERα promoter, and this association appeared stronger after E2 treatment but weaker after YAP1 knockdown. In vivo, E2 and the YAP activator XMU-MP-1 improved bone parameters in ovariectomized mice, and combined treatment showed the strongest rescue effect. Collectively, these data support a model in which E2 promotes BMSC osteogenesis, at least in part, through a YAP1/TEAD1-dependent increase in ERα expression. Because the ChIP assay used here was qualitative and reciprocal regulation of YAP1 by ERα was not directly tested, further quantitative and bidirectional mechanistic studies are warranted.</p>\u0000 </div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"57 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: The role and mechanism of 3D-printed hydrogel-CGF/PLGA composite polymer in promoting skin burn healing","authors":"Yue Guo,&nbsp;Yong Miao,&nbsp;ZhiQi Hu,&nbsp;Qian Qu","doi":"10.1007/s10735-026-10801-7","DOIUrl":"10.1007/s10735-026-10801-7","url":null,"abstract":"","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"57 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}