Journal of Molecular Histology最新文献

Histological study of the therapeutic effect of valerian extract alone or in combination with co-enzyme Q10 on experimentally induced gastric ulcers in adult albino rats: a light and scanning electron microscopic study. 缬草提取物单独或与辅酶Q10联合治疗实验性白化大鼠胃溃疡的组织学研究：光镜和扫描电镜研究。

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-05-28 DOI: 10.1007/s10735-025-10466-8

Heba G Ibrahim, Ghada A Meheissen, Amira M Omar

{"title":"Histological study of the therapeutic effect of valerian extract alone or in combination with co-enzyme Q10 on experimentally induced gastric ulcers in adult albino rats: a light and scanning electron microscopic study.","authors":"Heba G Ibrahim, Ghada A Meheissen, Amira M Omar","doi":"10.1007/s10735-025-10466-8","DOIUrl":"https://doi.org/10.1007/s10735-025-10466-8","url":null,"abstract":"<p><p>Gastric ulcer, affecting 10% of the global population, can lead to severe complications as perforation, bleeding, and fatal outcomes. Valerian extract and coenzyme Q10 show a potential as therapeutic agents that decrease inflammation and protect against oxidative damage. To evaluate the therapeutic effect of the co-administration of valerian extract and coenzyme Q10 versus the administration of each of them alone in ameliorating experimentally induced gastric ulcer. Rats were randomly assigned into the following groups: the control, the ulcer-induced, the treated groups: valerian extract, coenzyme Q10, and both treatments, all administered orally one hour after orally ingestion of ethanol, and finally untreated group. After the rats were sacrificed, their stomachs were analyzed macroscopically, histologically, and immunohistochemically. The ulcer-induced group demonstrated severe gastric damage with hyperemia and brownish hemorrhagic spots macroscopically, along with various histological changes, including deep gastric ulcers and multiple erosions. There was complete destruction of the fundic glands and their lining cells, areas of hemorrhage, loss of mucopolysaccharide content, and fibrosis which were confirmed by morphometrical studies. Scanning electron microscopy revealed wide ulcers and complete shedding of surface mucous epithelial cells. Valerian extract and coenzyme Q10 groups showed partial improvement with an increased rate of proliferation detected by immunohistochemical study. The combination of both drugs provided restoration of the gastric mucosa to a nearly control-like appearance. Combined treatment with valerian and Coenzyme Q10 was more effective than either alone in restoring the mucous barrier, regenerating epithelial cells, and reducing inflammation.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":"176"},"PeriodicalIF":2.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary intervention enhances fertility in obese male mice by regulating SLC2As. 饮食干预通过调节SLC2As提高肥胖雄性小鼠的生育能力。

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-05-28 DOI: 10.1007/s10735-025-10470-y

Liujia Sun, Xiaoqi Hong, Qi Zhu, Yu Xiang, Cen Xu, Lingling Weng, Jieyu Cai, Na Liang, Mingrui Xue, Hongshan Ge

{"title":"Dietary intervention enhances fertility in obese male mice by regulating SLC2As.","authors":"Liujia Sun, Xiaoqi Hong, Qi Zhu, Yu Xiang, Cen Xu, Lingling Weng, Jieyu Cai, Na Liang, Mingrui Xue, Hongshan Ge","doi":"10.1007/s10735-025-10470-y","DOIUrl":"https://doi.org/10.1007/s10735-025-10470-y","url":null,"abstract":"<p><p>Research has demonstrated that obesity can affect male fertility and reproductive potential, but the underlying mechanism remains unclear. This study aimed to investigate the effects of a high-fat diet (HFD) and dietary intervention on reproductive function, glucose metabolism, and related SLC2As in male mice. Forty 4-week-old male ICR mice were randomly divided into the normal diet (ND) group (group N, n = 15) and the HFD group (group F, n = 25). After 12 weeks, the mice were further divided into the following groups: ND maintenance group (NN group, n = 10), HFD maintenance group (FF group, n = 10), and transition to ND group (FN group, n = 10) through dietary intervention for 8 weeks. Intraperitoneal glucose tolerance test (IPGTT) was performed, and parameters including fasting blood glucose, body weight, sperm count, sperm motility, and testis and epididymis measurements were recorded. Testicular morphology was observed through hematoxylin-eosin staining. Western blot and immunofluorescence were used to detect the protein expression and localisation of SLC2As in the testis. Long-term HFD consumption resulted in increased body and testicular weights, decreased testicular and epididymal organ coefficients, reduced sperm motility rate, and increased area under the curve in the IPGTT test. After dietary intervention, compared to the NN group, the FF and FN groups exhibited increased testis weight, decreased testicular and epididymal organ coefficients, decreased sperm motility rate, reduced SLC2A3 and SLC2A8 protein expression levels in the FF group, and decreased SLC2A8 protein expression in the FN group. Obesity induced by HFD caused damage to the reproductive system of male mice and affected testicular glucose metabolism and the expression of sugar transporter SLC2As. Transitioning from HFD to ND can improve reproductive dysfunction caused by dietary obesity and its impact on sugar transporter protein expression to a certain extent.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":"174"},"PeriodicalIF":2.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cadmium-induced disruption of exosomal secretion and cellular pathways in leydig cells. 镉对间质细胞外泌体分泌和细胞通路的破坏。

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-05-28 DOI: 10.1007/s10735-025-10463-x

Waseem Ali, Atique Ahmed Behan, Yonggang Ma, Yan Chen, Hao Zheng, Zongping Liu, Hui Zou

{"title":"Cadmium-induced disruption of exosomal secretion and cellular pathways in leydig cells.","authors":"Waseem Ali, Atique Ahmed Behan, Yonggang Ma, Yan Chen, Hao Zheng, Zongping Liu, Hui Zou","doi":"10.1007/s10735-025-10463-x","DOIUrl":"https://doi.org/10.1007/s10735-025-10463-x","url":null,"abstract":"<p><p>Cadmium is a toxic heavy metal, disrupts different cellular secretions and induce pathological changes in the male reproductive system. However, cadmium-induced disruption of exosomal secretion and cellular pathways in Leydig cells is largely unknown. In this study, 30 C57BL/6 male mice were divided into two groups: one receiving purified water and the other 50 mg/L CdCl2 for three months. This is a first report, both in vivo and in vitro analyses showed that the control group exhibited strong immunoreactivity and immunosignaling with high secretion of exosomal proteins CD63 and multivesicular bodies (MVBs) through immunohistochemistry, immunofluorescence, and transmission electron microscopy. Leydig cells in the control group maintained a normal steroidogenic pathway, supporting the production of healthy, motile spermatozoa. Conversely, the cadmium-treated group showed irregularly dispersed Leydig cells with condensed nuclei and vacuolated mitochondria. Cadmium exposure led to reduced immunoreactivity, immunosignaling, and expression of CD63 in Leydig cells, with a noticeable lack of MVBs secretion. Additionally, cadmium significantly down-regulated the Steroidogenesis regulatory proteins STAR, CYP11A1, CYP17A1, 3BHSD1, 17BHSD1 and AR of Leydig cells. It also disrupted autophagic flux evidenced by increased expression of ATG5, ATG7, LC3, P62, and LAMP2 proteins. Furthermore, cadmium up-regulated apoptotic proteins (Caspase-3, Caspase-8, and Bax) and down-regulated the anti-apoptotic protein Bcl-2. This study provides novel insights into the detrimental effects of cadmium on Leydig cells' secretory pathways, highlighting disruptions in exosomal-MVBs secretion, autophagy and apoptosis, thereby posing significant risks to male fertility.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":"177"},"PeriodicalIF":2.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capsaicin counteracts dexamethasone-induced osteoporosis and metabolic disturbances in rats: role of AMPK/SIRT1/β-catenin/RUNX2 pathway. 辣椒素对抗地塞米松诱导的大鼠骨质疏松和代谢紊乱：AMPK/SIRT1/β-catenin/RUNX2通路的作用

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-05-28 DOI: 10.1007/s10735-025-10460-0

Esraa S Habiba, Mona Hassan Fathelbab, Eman M Omar, Aliaa M T ElAlkamy, Walaa Omar, Sahar A Harby

{"title":"Capsaicin counteracts dexamethasone-induced osteoporosis and metabolic disturbances in rats: role of AMPK/SIRT1/β-catenin/RUNX2 pathway.","authors":"Esraa S Habiba, Mona Hassan Fathelbab, Eman M Omar, Aliaa M T ElAlkamy, Walaa Omar, Sahar A Harby","doi":"10.1007/s10735-025-10460-0","DOIUrl":"https://doi.org/10.1007/s10735-025-10460-0","url":null,"abstract":"<p><p>Given their potent immunosuppressive and anti-inflammatory effects, long-term glucocorticoid therapy (GCs) is a common cause of bone fractures and secondary osteoporosis. Transient receptor potential vanilloid 1 (TRPV1) has been shown to play a role in preserving bone homeostasis and preventing bone disorders. The chili pepper is a naturally occurring source of capsaicin, a TRPV1 agonist. For this reason, this study compared the anti-resorptive properties of capsaicin with alendronate, the conventional treatment for osteoporosis, using a rat model of osteoporosis induced by dexamethasone (Dexa). Over six weeks, five groups of rats received the vehicle, Dexa alone (0.1 mg/kg, Sc), or Dexa plus either alendronate (1 mg/kg, orally) or capsaicin (1 or 2.5 mg/kg, orally). After the experiment, osteocalcin, RANKL, phosphorus, calcium, alkaline phosphatase (ALP), and metabolic parameters were measured. Furthermore, AMPK levels and the relative expression of Bax, Bcl-2, SIRT1, β-catenin, and RUNX2 were assessed in bone, and tissues from the femur were evaluated histologically. Capsaicin's effectiveness in alleviating the bone-damaging effect of dexamethasone was evident through a dose-dependent reduction in ALP, RANKL, and Bax, a rise in osteocalcin and Bcl-2, and a higher expression of AMPK, SIRT1, β-catenin, and RUNX2. Additionally, capsaicin improved bone architecture and effectively mitigated Dexa's detrimental metabolic impact on blood glucose and lipid profile. By upregulating the AMPK/SIRT1/β-catenin/RUNX2 pathway, capsaicin exhibits dose-dependent bone-stimulant effects in a dexamethasone-induced osteoporosis model in rats.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":"175"},"PeriodicalIF":2.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of TIGIT, PVRIG, CD112 and CD155 expression in early and late onset preeclampsia. TIGIT、PVRIG、CD112、CD155在早、晚发型子痫前期表达的研究

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-05-28 DOI: 10.1007/s10735-025-10459-7

Hülya Çetin, İlknur Lafcı, Yeliz Arman Karakaya, Buket Er Urgancı, Özlem Koşar Can

{"title":"Investigation of TIGIT, PVRIG, CD112 and CD155 expression in early and late onset preeclampsia.","authors":"Hülya Çetin, İlknur Lafcı, Yeliz Arman Karakaya, Buket Er Urgancı, Özlem Koşar Can","doi":"10.1007/s10735-025-10459-7","DOIUrl":"https://doi.org/10.1007/s10735-025-10459-7","url":null,"abstract":"<p><p>Preeclampsia is characterized by hypertension and proteinuria after the 20th week of pregnancy. The disease is divided into early and late onset according to the time of diagnosis. Early onset preeclampsia (EOP) develops after the 20th week of pregnancy. The late-onset form usually occurs after the 34th week of pregnancy. TIGIT and PVRIG are immune checkpoint inhibitor receptors. PVRIG binds only to the PVRL2 (nectin-2, CD112). TIGIT binds to both CD112 and CD155. In our study, the control group consisted of placentas from healthy pregnant women, the early onset preeclampsia group (EOP) consisted of patients diagnosed before the 34th week, and the late-onset preeclampsia group (LOP) consisted of placentas from patients diagnosed at or after the 34th week. TIGIT, PVRIG, CD155, and CD112 expression in placental materials was evaluated both immunohistochemically and by RT-PCR. As a result of H scoring of immunohistochemical expression, it was observed that CD112 and CD155 expression decreased and PVRIG expression increased when the EOP and LOP groups were compared with the control group. In the early onset preeclampsia group, CD112, CD155, TIGIT, and PVRIG gene expression increased twofold compared to that in the control group. In the late-onset preeclampsia group, the expression of all the genes decreased to one-third. The results of our study revealed that these genes may serve as biomarkers for early- and late-onset preeclampsia. Detailed studies are required to determine the use of these receptors in the diagnosis and treatment of the disease.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":"178"},"PeriodicalIF":2.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

hsa_circ_0001103 correlates with unfavorable MRI features and promotes glioma progression by modulating the miR-375/YAP1 axis hsa_circ_0001103与不利的MRI特征相关，并通过调节miR-375/YAP1轴促进胶质瘤进展

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-05-27 DOI: 10.1007/s10735-025-10445-z

Yu Hong, Lijun Liu, Yuedong Hai, Liwei Bao

{"title":"hsa_circ_0001103 correlates with unfavorable MRI features and promotes glioma progression by modulating the miR-375/YAP1 axis","authors":"Yu Hong, Lijun Liu, Yuedong Hai, Liwei Bao","doi":"10.1007/s10735-025-10445-z","DOIUrl":"10.1007/s10735-025-10445-z","url":null,"abstract":"<div><p>To investigate the role of circ_0001103 in glioma and its underlying molecular mechanism. Differentially expressed circRNAs in glioblastoma cells and neural progenitor cells were analyzed with the data of GSE146463. Tissues samples, clinicopathological parameters and MRI characteristics were retrospectively collected. Circ_0001103, miR-375 and YAP1 expression levels were measured by qRT-PCR and Western blot. The relationship between circ_0001103 expression and clinicopathological parameters or MRI features of glioma patients was analyzed by chi-square test. After circ_0001103 or miR-375 was selectively up-regulated or down-regulated, CCK-8 assay, BrdU assay and flow cytometry were used to detect the proliferation and apoptosis of glioma cells. Bioinformatics prediction, dual-luciferase reporter gene assay and RNA immunoprecipitation were executed to verify the targeting relationships among miR-375, circ_0001103 and YAP1 3’ UTR. circ_0001103 was significantly up-regulated in glioma tissues and cell lines, which was positively correlated with the larger tumor size and non-uniform signal in MRI. Overexpression of circ_0001103 promoted glioma cell proliferation and inhibited apoptosis, while knockdown of circ_0001103 worked oppositely. Circ_0001103 negatively regulated miR-375 expression. MiR-375 targeted YAP1, and YAP1 was indirectly and positively modulated by circ_0001103. Circ_0001103 expression was negatively correlated with miR-375 expressions, while positively correlated with YAP1 expression in glioma tissues. Circ_0001103 contributes to glioma progression by regulating miR-375/YAP1 axis.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-29a-3p compositely regulates the COL6A6/PTEN-PI3K/Akt/CUX1 feedback loop to participate in the proliferation and invasion of pituitary adenomas miR-29a-3p复合调控COL6A6/PTEN-PI3K/Akt/CUX1反馈回路参与垂体腺瘤的增殖和侵袭

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-05-27 DOI: 10.1007/s10735-025-10436-0

Zhuohui Liu, Xiufu Liao, Hexiang Zhao, Biao Ruan, Fengfeng Jia, Xuzhi He, Ruiqing Long

{"title":"miR-29a-3p compositely regulates the COL6A6/PTEN-PI3K/Akt/CUX1 feedback loop to participate in the proliferation and invasion of pituitary adenomas","authors":"Zhuohui Liu, Xiufu Liao, Hexiang Zhao, Biao Ruan, Fengfeng Jia, Xuzhi He, Ruiqing Long","doi":"10.1007/s10735-025-10436-0","DOIUrl":"10.1007/s10735-025-10436-0","url":null,"abstract":"<div><p>Pituitary adenoma (PA) is one of the most common intracranial tumors, and owing to its special biological morphology and behavior, there is currently no effective treatment. miRNAs play crucial roles as diagnostic indicators and targets for the treatment of numerous cancer types. The objective of this research was to explore how miR-29a-3p influences the development of PA. We collected 25 pairs of PA tissue and normal pituitary tissue, followed by the subcutaneous injection of 5 × 10<sup>7</sup> HP75 cells into the left axilla of nude mice, creating a heterotopic PA xenograft tumor model for experimental study. TtT/GF and HP75 cell proliferation and tumor growth in nude mice were assessed using CCK-8, Transwell, and immunohistochemistry tests. Western blotting, RT‒qPCR and RIP were used to detect the expression and interaction of related proteins and genes. The expression of miR-29a-3p was upregulated in PA. Knockdown of miR-29a-3p can inhibit the proliferation, invasion and migration of TtT/GF and HP75 cells and reduce the epithelial mesenchymal transformation (EMT) of these cells. Furthermore, reducing miR-29a-3p levels suppressed the expression of Ki-67 in the PA tissues of nude mice and slowed tumor growth. From a mechanistic standpoint, miR-29a-3p can target COL6A6 and PTEN. Knockdown of miR-29a-3p inhibits the PI3K/Akt/CUX1 signaling pathway through simultaneously increasing COL6A6 and PTEN expression, thus inhibiting the proliferation, invasion, migration and EMT of PA cells and alleviating the progression of PA. Conversely, CUX1 can promote the expression of miR-29a-3p through a positive feedback loop and accelerate the development of PA. Our study suggests that downregulating the expression of miR-29a-3p may be a new target for the treatment of PA.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10735-025-10436-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long non-coding RNA HOXA11-AS inhibits apoptosis, induces proliferation, and promotes autophagy via the miR-214-3p/ATG12 axis in acute T lymphoblastic leukemia 在急性T淋巴细胞白血病中，长链非编码RNA HOXA11-AS通过miR-214-3p/ATG12轴抑制细胞凋亡，诱导细胞增殖，促进细胞自噬

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-05-26 DOI: 10.1007/s10735-025-10462-y

Rui Zhang, YuanYuan Zhang, XiaoFei Li, ShaoHua Wang, Jiao Li, ShuoChun Shao, Bin Liu, WeiWei Guo, Min Shi

{"title":"Long non-coding RNA HOXA11-AS inhibits apoptosis, induces proliferation, and promotes autophagy via the miR-214-3p/ATG12 axis in acute T lymphoblastic leukemia","authors":"Rui Zhang, YuanYuan Zhang, XiaoFei Li, ShaoHua Wang, Jiao Li, ShuoChun Shao, Bin Liu, WeiWei Guo, Min Shi","doi":"10.1007/s10735-025-10462-y","DOIUrl":"10.1007/s10735-025-10462-y","url":null,"abstract":"<div><p>Acute T lymphocytic leukemia (T-ALL) is a hematological cancer with high mortality. The literature suggests an association between T-ALL and Long non-coding RNAs (lncRNAs). Nevertheless, the mechanism of lncRNA HOXA11-AS in T-ALL remains undetermined. The lncRNA HOXA11-AS expression level were assessed in T-ALL patients and normal controls by qRT-PCR. Furthermore, The proliferation, apoptosis, and autophagy of T-ALL cell (Molt4 and Jurkat cells) were investigated in <i>vitro via</i> EdU incorporation experiment, flow cytometry, immunofluorescence, and Western blotting. Moreover, the relationship between HOXA11-AS and miR-214-3p and between miR-214-3p, and <i>ATG12</i> was verified <i>via</i> dual-luciferase reporter assays. Our investigation demonstrated that compared to normal controls, the HOXA11-AS expression level were increased in T-ALL patients. Furthermore, in T-ALL cells, inhibition of HOXA11-AS or overexpression of miR-214-3p reduced autophagy and proliferation, while stimulating apoptosis. However, miR-214-3p inhibition counteracted HOXA11-AS-mediated suppression of T-ALL cell proliferation, autophagy, and apoptosis. In addition, HOXA11-AS modulated <i>ATG12 via</i> sponging miR-214-3p. Overall, this research indicated that lncRNA HOXA11-AS not only stimulates cell proliferation and autophagy but also inhibits apoptosis <i>via</i> the miR-214-3p/ATG12 axis, thereby suggesting that lncRNA HOXA11-AS might be a new candidate for T-ALL diagnosis and therapy.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical and histopathological changes in ovary, uterus and testicular tissues after acrylamide exposure 丙烯酰胺暴露后卵巢、子宫和睾丸组织的生化和病理变化

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-05-26 DOI: 10.1007/s10735-025-10456-w

Hamit Uslu, Gözde Atila Uslu, Taha Abdulkadir Çoban, Ali Sefa Mendil, Emine Toraman, Mahmut Şahin, Mustafa Özkaraca

{"title":"Biochemical and histopathological changes in ovary, uterus and testicular tissues after acrylamide exposure","authors":"Hamit Uslu, Gözde Atila Uslu, Taha Abdulkadir Çoban, Ali Sefa Mendil, Emine Toraman, Mahmut Şahin, Mustafa Özkaraca","doi":"10.1007/s10735-025-10456-w","DOIUrl":"10.1007/s10735-025-10456-w","url":null,"abstract":"<div><p>Acrylamide (ACR) is a popular substance to which our exposure increases with the changes in our lifestyle and brings with it various health problems. In order to determine appropriate therapeutics against ACR damage, it is important to investigate the multiple mechanisms that may be effective in its pathophysiology. This study investigated the effects of ACR exposure on ovarian, uterine, and testicular tissues by considering different pathophysiological pathways. Male-control (MC), male-acrylamide (MACR), female-control (FC), and female-acrylamide (FACR) groups were formed. ACR was administered at a dose of 60 mg/kg for 5 days. ACR exposure decreased CAT and TrxR-specific activities, GSH levels, and Bcl-2 expression, while significantly increasing MDA, IL-6, and NFĸB p65 levels, caspase 3, and Bax expression in ovarian, uterine, and testicular tissues. Based on these results, it was determined that acrylamide induced damage in ovarian, uterine and testicular tissues through various pathways such as oxidative stress, inflammation, and apoptosis. Consequently, when selecting a therapeutic target, the substance whose efficacy is being investigated should be effective in these pathways. Furthermore, this study is the first to demonstrate the occurrence of bladder retention in both sexes following acrylamide exposure and will be an important step for future research.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><img></picture></div></div></figure></div></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circ_0008285 regulates macrophage polarization through miR-375/MAPK14 axis in sepsis-induced acute lung injury Circ_0008285在脓毒症诱导的急性肺损伤中通过miR-375/MAPK14轴调控巨噬细胞极化

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-05-26 DOI: 10.1007/s10735-025-10465-9

Chen Li, Jianhua Liu, Gaixia Feng, Jing Su, Kailun Xu, Zhihua Zhang

{"title":"Circ_0008285 regulates macrophage polarization through miR-375/MAPK14 axis in sepsis-induced acute lung injury","authors":"Chen Li, Jianhua Liu, Gaixia Feng, Jing Su, Kailun Xu, Zhihua Zhang","doi":"10.1007/s10735-025-10465-9","DOIUrl":"10.1007/s10735-025-10465-9","url":null,"abstract":"<div><p>Acute lung injury (ALI) induced by sepsis is a serious life-threatening disease, one of its characteristics is the polarization of macrophages. Circ_0008285 has been found to be associated with various diseases. In this study, we detected the regulatory role and mechanism of circ_0008285 in sepsis-induced ALI. RAW264.7 cells treated with LPS and C57BL/6 male mice were used to construct in vitro and in vivo models, respectively. Through A series of experiments such as qRT-PCR, Western blot, CCK-8, flow cytometry, dual-luciferase reporter experiment, HE staining and TUNEL staining, the role of circ_0008285 in sepsis-induced ALI was explored. In LPS-induced RAW264.7 cell, circ_0008285 and MAPK14 were over-expressed, but miR-375 was low-expressed compared with control. The levels of IL-1β, IL-6, TNF-α, iNOS and CD86 were reduced, but CD206 and Arg1 expression were enhanced both in vitro and in vivo after knockdown of circ_0008285. In TC-1 cell co-cultured with LPS+sh-circ_0008285 cells, the viability was increased and the apoptosis level was decreased compared with LPS+sh-NC. Circ_0008285 was the sponge of miR-375, and MAPK14 was the downstream target of miR-375. The injury score, W/D ratio, MPO level and apoptosis level in lung tissue were decreased after knockdown of circ_0008285. Moreover, the total protein, neutrophils and macrophages in BALF were increased. Collectively, this study identified that circ_0008285 could sponge miR-375 to influence MAPK14 expression, and then regulate macrophage polarization of sepsis-induced ALI, which provided new insights for the treatment of sepsis-induced ALI.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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