Journal of Molecular Histology最新文献

A determination of the main regulators of necroptosis in testicular tissue under different heat stresses.

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-01-25 DOI: 10.1007/s10735-024-10350-x

Musa Tatar, Kıymet Kübra Tüfekci, Sema Uslu

{"title":"A determination of the main regulators of necroptosis in testicular tissue under different heat stresses.","authors":"Musa Tatar, Kıymet Kübra Tüfekci, Sema Uslu","doi":"10.1007/s10735-024-10350-x","DOIUrl":"https://doi.org/10.1007/s10735-024-10350-x","url":null,"abstract":"<p><p>Although minimal increases in testicular temperature can compromise spermatogenesis and lead to fertility-related problems, the basic mechanism involved in germ cell destruction as a response to heat stress is still unclear. However, necroptosis is known to regulate a number of physiological and pathological events. This study investigated the role of RIPK1/RIPK3 and MLKL, the main regulators of necroptosis, against different heat stresses in testis tissue. Forty-two Wistar albino rats were divided into seven groups: six experimental exposed to heat stress and one control. Heat stress was induced by causing the rats to swim for 30 min daily for 60 days in a water bath at temperatures of 39 °C and 43 °C. Testis tissues were collected while the animals were under anesthesia on the 1st, 7th, and 14th days after 60 days of heat application. The tissues were first fixed in Bouin's solution. After routine histological procedures, immunohistochemical staining was performed on one-half of the tissues using RIPK1/RIPK3 and MLKL primary antibodies on serially collected 5 μm-thick sections. Immunoblotting analysis was performed on the other half. Analyses revealed an increase in the expression of RIPK1/RIPK3 and MLKL proteins, regulators of necroptosis, in both the 39 °C and 43 °C groups, although this was greater in the tissue exposed to 43 °C heat stress. These molecules were also especially affected by round and elongated spermatids, and reactivity was observed in Leydig cells. In conclusion, exposure to increased temperature may cause RIPK1/RIPK3 and MLKL-mediated cellular changes in the testis.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 1","pages":"74"},"PeriodicalIF":2.9,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Exosomes derived from human exfoliated deciduous teeth ameliorate adult bone loss in mice through promoting osteogenesis.

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-01-25 DOI: 10.1007/s10735-025-10352-3

Jizhen Wei, Yeqing Song, Zhihao Du, Feiyan Yu, Yimei Zhang, Nan Jiang, Xuejun Ge

引用次数: 0

Persimmon (Diospyros kaki L.) leaves accelerates skin tissue regeneration in excisional wound model: possible molecular mechanisms.

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-01-24 DOI: 10.1007/s10735-024-10304-3

Talal Salem H Al-Qaisi, Ahmed A J Jabbar, Mohammed M Hussein M Raouf, Parween AbdulSamad Ismail, Ramzi A Mothana, Mohammed F Hawwal, Rawaz Rizgar Hassan, Mahmood Ameen Abdulla, Musher Ismael Saleh, Mohammed Awad

{"title":"Persimmon (Diospyros kaki L.) leaves accelerates skin tissue regeneration in excisional wound model: possible molecular mechanisms.","authors":"Talal Salem H Al-Qaisi, Ahmed A J Jabbar, Mohammed M Hussein M Raouf, Parween AbdulSamad Ismail, Ramzi A Mothana, Mohammed F Hawwal, Rawaz Rizgar Hassan, Mahmood Ameen Abdulla, Musher Ismael Saleh, Mohammed Awad","doi":"10.1007/s10735-024-10304-3","DOIUrl":"https://doi.org/10.1007/s10735-024-10304-3","url":null,"abstract":"<p><p>Persimmon (Diospyros kaki L.) leaves are a traditional medicinal herb used for treating many infectious and inflammatory-related conditions, including wound healing. To validate its traditional use, our study evaluates the acute toxicity and wound-healing effects of methanolic extracts of Persimmon (Diospyros kaki L.) leaves (MEPL) on excisional neck injury in rats. A uniform dorsal neck injury was created for twenty-four Sprague Dawley rats, which were randomly aligned into 4 groups and treated topically twice daily with 0.2 ml of the following: group A, rats treated with 1% CMC; group B, rats received intrasite gel; groups C and D, rats treated with MEPL (0.2 ml of 250 and 500 mg/kg, respectively). The toxicity results showed a lack of physiologic alteration or mortality in rats ingested with an oral dosage of up to 5 g/kg of MEPL. Histological screening of regenerated skin tissues revealed higher deposition of collagen, fibroblast cells, and reduced inflammatory cells in MEPL-treated rats. The topical application of MEPL led to positive modulation of Transforming Growth Factor Beta 1 (angiogenetic factor) in wound tissues, indicating increased tissue regeneration and faster wound contraction. MEPL treatment caused a significant elevation of tissue antioxidants (superoxide dismutase and catalase) and hydroxyproline (collagen) contents while reducing malondialdehyde contents. The inflammatory mediators (TNF-α and IL-6) were lower, and anti-inflammatory cytokines (interleukin 10) were higher in MEPL-treated rats than in the vehicle group. The study outcomes back up the traditional use of MEPL for wound healing, which could be linked with its phytochemicals (flavonoids and terpenoids) that require further isolation and molecular identification.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 1","pages":"73"},"PeriodicalIF":2.9,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LINC02418 suppresses endometrial cancer progression via regulating miR-494-3p/RASGRF1 axis LINC02418通过调节miR-494-3p/RASGRF1轴抑制子宫内膜癌进展

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-01-22 DOI: 10.1007/s10735-024-10327-w

Hongfeng Li, Jia Bian, Minjie Liu, Yijie Wang, Yapping Shang, Yu Zheng, Xuehe Li

{"title":"LINC02418 suppresses endometrial cancer progression via regulating miR-494-3p/RASGRF1 axis","authors":"Hongfeng Li, Jia Bian, Minjie Liu, Yijie Wang, Yapping Shang, Yu Zheng, Xuehe Li","doi":"10.1007/s10735-024-10327-w","DOIUrl":"10.1007/s10735-024-10327-w","url":null,"abstract":"<div><p>Long non-coding RNAs (lncRNAs) have emerged as pivotal regulatory molecules in cancer biology. Among these, long intergenic non-protein coding RNA 02418 (LINC02418), a recently identified lncRNA, has been linked to endometrial cancer (EC), although its function and operational mechanisms are largely unclear. The present investigation aims to elucidate the molecular mechanism through which LINC02418 influences EC pathogenesis. We employed Western blotting and quantitative real-time PCR to analyze Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1) and LINC02418 expression profiles in EC tissues and cell lines. Functional analyses, including cell proliferation, migration, and invasion assays, were conducted to evaluate the impact of LINC02418 overexpression on EC cells. Xenograft mouse models were established for in vivo validation. The molecular interactions between LINC02418, miR-494-3p, and RASGRF1 were characterized using luciferase reporter and RNA pull-down assays. LINC02418 expression was significantly downregulated in EC tissues and cell lines compared to their normal counterparts. Forced expression of LINC02418 significantly suppressed EC cell proliferation, migration, and invasion in vitro. In xenograft models, LINC02418 overexpression resulted in reduced tumor burden and enhanced cell death. Mechanistically, LINC02418 enhanced RASGRF1 expression by sequestering miR-494-3p, a finding substantiated by RNA pull-down assays. The tumor-suppressive effects of LINC02418 were partially reversed by RASGRF1 silencing and miR-494-3p overexpression. Clinical analyses revealed that reduced RASGRF1 expression correlated with poor histological differentiation, advanced tumor stages, and decreased overall survival in EC patients. Our findings establish LINC02418 as a tumor suppressor that regulates EC progression through modulation of the miR-494-3p/RASGRF1 axis, highlighting its potential as a therapeutic target in EC treatment.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gallic acid mitigates lipopolysaccharide-induced testicular inflammation via regulation of the NF-κB and PK2/PKR1 pathway 没食子酸通过调节NF-κB和PK2/PKR1通路减轻脂多糖诱导的睾丸炎症

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-01-18 DOI: 10.1007/s10735-024-10349-4

Ozlem Delen, Yesim Hulya Uz, Cengiz Yuksel, Onur Ersoy, Gulnur Kizilay

{"title":"Gallic acid mitigates lipopolysaccharide-induced testicular inflammation via regulation of the NF-κB and PK2/PKR1 pathway","authors":"Ozlem Delen, Yesim Hulya Uz, Cengiz Yuksel, Onur Ersoy, Gulnur Kizilay","doi":"10.1007/s10735-024-10349-4","DOIUrl":"10.1007/s10735-024-10349-4","url":null,"abstract":"<div><p>Genital tract infections are common causes of male infertility, and most of diagnosed men are asymptomatic. This study examined the effect of gallic acid (GA) against lipopolysaccharide (LPS)-induced testicular inflammation. Thirty-two <i>Spraque Dawley</i>, 2.5-3 month-old male rats were separated into four groups (<i>n</i> = 8). Control group; saline at 3 ml/kg, and in the GA group; GA was dissolved in saline, by gavage at 100 mg/kg for 14 days. LPS group; LPS 5 mg/kg as a single dose was given intraperitoneal on the 11th day. LPS + GA group; GA was given for 14 days and LPS 5 mg/kg on the 11th day. After 72 h of LPS injection, all samples were collected. Semen analysis, biochemical assays, histological evaluations, and immunohistochemical or Western blot analyses for nuclear factor-kappa B (NF-κB) and Prokineticin 2/prokineticin receptor 1(PK2/PKR1) pathways were performed. There was a significant decrease in body and testicular weight, sperm parameters, serum testosterone level, mean seminiferous tubule diameter, germinal epithelial thickness, and Johnsen score in the LPS group compared to control and GA groups. However, a significant increase was found in interstitial space width, percentage of abnormal sperm, NF-κB and PK2 immunoreactivities, and expression of PK2 and PKR1 proteins. In the LPS + GA group, GA administration was observed to significantly prevent these adverse effects. In conclusion, the inhibitory effects of GA on the NF-κB and PK2/PKR1 pathways not only suppressed the inflammatory response but also restored impaired sperm parameters and testicular structure. These findings indicate GA’s potential for treating testicular inflammation and protecting male reproductive health.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis with stem cell therapy in rats with pharyngocutaneous fistula formation 与干细胞治疗大鼠咽皮瘘形成的比较分析。

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-01-15 DOI: 10.1007/s10735-024-10343-w

Aykut Ceyhan, Muhammed Fatih Topuz, Orhan Ozatik, Suna Karadenız Saygılı

{"title":"Comparative analysis with stem cell therapy in rats with pharyngocutaneous fistula formation","authors":"Aykut Ceyhan, Muhammed Fatih Topuz, Orhan Ozatik, Suna Karadenız Saygılı","doi":"10.1007/s10735-024-10343-w","DOIUrl":"10.1007/s10735-024-10343-w","url":null,"abstract":"<div><p>Laryngocutaneous fistula is one of the most important complications encountered after larynx surgery. Stem cell therapy is a promising treatment approach for the future, both without the need for surgical methods and by assisting surgical methods to close the fistula. 30 female Downey Sprague rats were divided into 5 separate groups and pharyngocutaneous fistula was created. Stem cells were administered subcutaneously to Group 1(SC-SC) after the creation of PCF, and via the tail vein to Group 2 (V-SC). The 3rd group (C) was made as a control group and while fistula formation and subsequent closure characteristics in the neck were monitored without any treatment, the rats in the 4th group (V-M/SH) were given tail vein medium/sham and the 5th group (SC-M/SH) was given as subcutaneous medium/sham group. PCF closure was significantly higher in the group given SC compared to the control group. The differences between the groups were examined by Hematoxilen Eosin staining with light microscopy and evaluated in terms of inflammatory cell infiltration, fibroblastic activity, collagen accumulation and neo-angiogenesis using the Ehrlich Hunt scale. After the experiments, all groups were stained with immunocytochemistry via TGF-β, VEGF and TNF-α antibodies. The statistical analysis was performed using the One-Way ANOVA test. When all these parameters were compared with the control group, a statistically significant difference was found.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiRNAs and tempol therapeutic potential in prostate cancer: a preclinical approach mirna和前列腺癌的临时治疗潜力：临床前方法

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-01-13 DOI: 10.1007/s10735-024-10341-y

Isabela Maria Urra Rossetto, Letícia F. Alves, Leonardo A. Marson, Murilo V. Geraldo, Felipe R. Santos, Fábio Montico, Valéria H. A. Cagnon

{"title":"MiRNAs and tempol therapeutic potential in prostate cancer: a preclinical approach","authors":"Isabela Maria Urra Rossetto, Letícia F. Alves, Leonardo A. Marson, Murilo V. Geraldo, Felipe R. Santos, Fábio Montico, Valéria H. A. Cagnon","doi":"10.1007/s10735-024-10341-y","DOIUrl":"10.1007/s10735-024-10341-y","url":null,"abstract":"<div><p>This study investigated tempol action on genes and miRNAs related to NFκB pathway in androgen dependent or independent cell lines and in TRAMP model in the early and late-stages of cancer progression. A bioinformatic search was conducted to select the miRNAs to be measured based on the genes of interest from NFκB pathway. The <i>miR-let-7c-5p</i>, <i>miR-26a-5p</i> and <i>miR-155-5p</i> and five target genes (BCL2, BCL2L1, RELA, TNF, PTGS2) were chosen for RT-PCR and gene enrichment analyses. In vitro, PC-3 and LNCaP cells were exposed, respectively, to 1.0 or 2.0 mM of tempol during 48 h. In vivo, five experimental groups were evaluated regarding tempol effects in the early (CT12 and TPL12 groups) and late-stages (CT20, TPL20-I and TLP20-II) of PCa development. TPL groups were treated with 50 mg/kg or 100 mg/kg of tempol. The ventral lobe of the prostate and the plasma was collected. Tempol treatment increased miRs expression in PC-3 and LNCaP. For both cell lines, tempol decreased RELA expression. In TRAMP model, tempol increased miRNA expression in prostate for all treated groups. Tempol upregulated the miRNA expressions related to the NFκB pathway in the prostate tissue and human tumor cell lines. Their increase is mainly linked to increased cell death and delayed CaP aggressivenes. Thus, tempol’s capacity for miRNA-mediated gene silencing to decrease tissue proliferation and cell survival processes is part of its tissue mechanics.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of the WNT4/ β-catenin/FOXO1 pathway by PDK1 promotes cervical cancer metastasis and EMT process PDK1激活WNT4/ β-catenin/FOXO1通路促进宫颈癌转移和EMT过程

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-01-09 DOI: 10.1007/s10735-024-10342-x

Shidong Chen, Cuixia Zhang, Honglang Huang, Yuhuan Wang, Mingjian Lian, Guolin Hong

{"title":"Activation of the WNT4/ β-catenin/FOXO1 pathway by PDK1 promotes cervical cancer metastasis and EMT process","authors":"Shidong Chen, Cuixia Zhang, Honglang Huang, Yuhuan Wang, Mingjian Lian, Guolin Hong","doi":"10.1007/s10735-024-10342-x","DOIUrl":"10.1007/s10735-024-10342-x","url":null,"abstract":"<div><h3>Objective</h3><p>This study aimed to elucidate the role of pyruvate dehydrogenase kinase-1 (PDK1) in cervical cancer (CC) by investigating its impact on cell proliferation, migration, and epithelial-mesenchymal transition (EMT) under hypoxic conditions.</p><h3>Methods</h3><p>PDK1-silenced CC cell lines were established using lentiviral shRNA technology. Cell migration and invasion were assessed through scratch and Transwell assays, respectively. Cellular activity and apoptosis-related protein expression levels were evaluated using MTT assays and western blotting. Transcriptome sequencing elucidates the regulatory pathways impacted by PDK1 silencing, and rescue experiments confirmed the underlying mechanisms. Xenograft models with nude mice were used to validate the effects of PDK1 silencing on CC progression.</p><h3>Results</h3><p>PDK1 silencing reduced migration, invasion, and cellular activity under hypoxic conditions while promoting apoptosis. Transcriptomic analysis revealed that PDK1 suppression downregulated the WNT4/β-catenin/FOXO1 pathway, decreasing EMT-related protein expression. Mechanistically, PDK1 enhanced β-catenin stability by inhibiting its phosphorylation through AKT-mediated GSK3β inactivation, promoting EMT and anti-apoptotic gene transcription.</p><h3>Conclusions</h3><p>Targeting PDK1 may provide novel therapeutic strategies specifically for CC by modulating the WNT4/β-catenin/FOXO1 pathway and associated EMT and apoptotic processes.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142939357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential role of SCF combined with DPCs in facial nerve repair SCF联合DPCs在面神经修复中的潜在作用

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-01-07 DOI: 10.1007/s10735-024-10351-w

Jinjie Ma, Jing Yan, Nan Su, Zhengjun Qiu, Huailong Hou, Jingxuan Sun, Xiangyu Sun, Yumei Niu, Lina He

{"title":"The potential role of SCF combined with DPCs in facial nerve repair","authors":"Jinjie Ma, Jing Yan, Nan Su, Zhengjun Qiu, Huailong Hou, Jingxuan Sun, Xiangyu Sun, Yumei Niu, Lina He","doi":"10.1007/s10735-024-10351-w","DOIUrl":"10.1007/s10735-024-10351-w","url":null,"abstract":"<div><p>Facial nerve injuries lead to significant functional impairments and psychological distress for affected patients. Effective repair of these injuries remains a challenge. For longer nerve gaps, the regeneration outcomes after nerve grafting remain suboptimal due to limited sources and postoperative immune responses. Tissue engineering techniques are conventional methods for repairing peripheral nerve defects. This study explores the potential of dental pulp cells (DPCs) combined with stem cell factor (SCF) to enhance neurogenic differentiation and improve facial nerve regeneration. DPCs were isolated from rabbit dental pulp, the pluripotency of the cells was identified from three perspectives: osteogenic differentiation, adipogenic differentiation, and neurogenic differentiation. In vivo experiments involved injuring the buccal branch of the facial nerve in New Zealand white rabbits, followed by treatment with PBS, DPCs, SCF, or SCF + DPCs. Functional recovery was assessed over 12 weeks, with SCF + DPCs demonstrating the most significant improvement in whisker movement scores. Histomorphological evaluations revealed enhanced myelinated fiber density and axonal morphology in the SCF + DPCs group. RNA sequencing identified 608 differentially expressed genes, with enrichment in the TGF-β signaling pathway. In in vitro experiments, we demonstrated from multiple angles using Western blot analysis, Real-time quantitative polymerase chain reaction (QPCR) analysis, and immunofluorescence staining that SCF can promote the neurogenic differentiation of DPCs through the TGF-β1 signaling pathway. Our findings indicate that the combination of SCF and DPCs offers a promising strategy for enhancing facial nerve repair.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Additional benefits of combined ceftriaxone and adipose-derived mesenchymal stem cells on revamping the outcomes in rodent after acute spinal infection 头孢曲松联合脂肪源性间充质干细胞对改善急性脊髓感染后啮齿动物预后的额外益处

IF 2.9 4区生物学

Journal of Molecular Histology Pub Date : 2025-01-06 DOI: 10.1007/s10735-024-10344-9

Tsung-Cheng Yin, Hung-Sheng Lin, Pei-Hsun Sung, John Y. Chiang, Chien-Hui Yang, Hon-Kan Yip, Kuan-Hung Chen

{"title":"Additional benefits of combined ceftriaxone and adipose-derived mesenchymal stem cells on revamping the outcomes in rodent after acute spinal infection","authors":"Tsung-Cheng Yin, Hung-Sheng Lin, Pei-Hsun Sung, John Y. Chiang, Chien-Hui Yang, Hon-Kan Yip, Kuan-Hung Chen","doi":"10.1007/s10735-024-10344-9","DOIUrl":"10.1007/s10735-024-10344-9","url":null,"abstract":"<div><p>This study tested whether combined ceftriaxone and adipose-derived mesenchymal stem cells (ADMSCs) would defend the spinal cord against acute spinal infection (ASI) in rodent. Adult-Male-SD rats were grouped into groups 1 (SC)/2 (ASI)/3 (ASI + ceftriaxone from days 2 to 28 after ASI induction)/4 (ASI + allogenic ADMSCs from day 2 for a total of 3 doses/3 consecutive intervals by intravenous injection)/5 (ASI + combined ceftriaxone and ADMSC) and spinal cord tissues were harvested by day 28. Circulatory levels of TNF-α/IL-6 at days 7 and 28, and these two parameters in spinal fluid at day 28 were lowest in group 1, highest in group 2, significantly lower in group 5 than in groups 3/4, and significantly lower in group 3 than in group 4 (all <i>p</i> < 0.0001). The day-28 bacterial colony formation unit (CFU) in vertebral bone and circulatory WBC counts at the time points of days 7/14/28, and the protein expressions of upstream (TRL-2/TLR-4/MYD88/TRAF6/IKKα/IKKβ /IKBβ/p-NF-κB) and downstream (IL-1β/IL-6/TNF-α/IFN-γ/iNOS) inflammatory signalings displayed a similar pattern of inflammatory biomarkers in spinal fluid among the groups (all <i>p</i> < 0.0001). By day 28, the bone injury score/bone marrow density/ratio of bone volume (BV) to the bone tissue volume (TV)/ratio of bone surface (BS) to BV/ratio of BS to bone TV/trabecular number exhibited an opposite, whereas the trabecular space exhibited an alike pattern of inflammatory biomarkers among the groups (all <i>p</i> < 0.0001). Combined ceftriaxone and ADMSCs therapy offered an additional benefit on protecting the vertebral bone/spinal cord against ASI damage.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10735-024-10344-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0