miR-429通过靶向ZEB1抑制唾液腺样囊性癌的进展和转移。

IF 2.9 4区 生物学 Q3 CELL BIOLOGY
Juan Li, Hongwei Zhao
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引用次数: 0

摘要

miR-429是一种肿瘤抑制因子,已在多种癌症中被观察到。miR-429影响唾液腺样囊性癌(SACC)的确切机制尚不完全清楚。首先,通过实时定量聚合酶链反应检测SACC患者肿瘤组织和匹配的邻近非肿瘤组织中miR-429和锌指E-box-binding homeobox 1 (ZEB1)的表达。接下来,使用CCK-8、伤口愈合、transwell和背根神经节(DRG)共培养模型来探索miR-429对SACC细胞增殖、迁移、侵袭和神经周围侵袭(PNI)的影响。最后,通过生物信息学分析和双荧光素酶报告基因分析筛选并验证miR-429的下游靶基因,探讨miR-429对SACC细胞上皮-间充质转化(epithelial-mesenchymal transition, EMT)的调控作用。miR-429在SACC肿瘤组织中低表达,ZEB1在SACC肿瘤组织中大量表达。miR-429水平升高可显著抑制SACC细胞的增殖、迁移、侵袭和PNI。筛选并验证了ZEB1作为miR-429的下游靶基因。高浓度的miR-429也显著降低了SACC细胞中ZEB1的表达,从而抑制了EMT。这些结果表明,miR-429在SACC中低表达,可能抑制SACC细胞的进展和转移,并且与ZEB1和EMT通路相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
miR-429 suppresses progression and metastasis of salivary adenoid cystic carcinoma by targeting ZEB1.

miR-429 is a tumor suppressor that has been observed in various cancers. The exact mechanism by which miR-429 affects salivary adenoid cystic carcinoma(SACC) is not fully understood. Initially, the expression of miR-429 and zinc finger E-box-binding homeobox 1 (ZEB1) in tumor tissues and matched adjacent non-tumor tissues of SACC patients were inspected by quantitative real-time polymerase chain reaction. Next, CCK-8, wound healing, transwell, and dorsal root ganglion (DRG) co-culture models were used to explore the effects of miR-429 on SACC cell proliferation, migration, invasion, and perineural invasion (PNI). Finally, the downstream target genes of miR-429 were screened and validated through bioinformatics analysis and dual-luciferase reporter analysis, and the regulatory role of miR-429 on epithelial-mesenchymal transition (EMT) in SACC cells was explored. miR-429 was poorly expressed while ZEB1 was substantially expressed in SACC tumor tissues. Elevated levels of miR-429 led to a significant suppression of SACC cell of proliferation, migration, invasion, and PNI. ZEB1 was screened and validated as a downstream target gene of miR-429. High concentrations of miR-429 also markedly lowered the expression of ZEB1 in SACC cells, thereby inhibiting EMT. These results suggest that miR-429 is lowly expressed in SACC, may suppress progression and metastasis of SACC cells, and is associated with ZEB1 and the EMT pathway.

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来源期刊
Journal of Molecular Histology
Journal of Molecular Histology 生物-细胞生物学
CiteScore
5.90
自引率
0.00%
发文量
68
审稿时长
1 months
期刊介绍: The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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