大戟对创伤模型的治疗作用:细胞凋亡、氧化应激和炎症分子的作用。

IF 2.9 4区 生物学 Q3 CELL BIOLOGY
Hanan Ibrahim Althagbi, Khalid M Alqaisi, Noralhuda Ayad Ibrahim, Parween Abdul-Samad Ismail, Ahmed A J Jabbar, Rawaz Rizgar Hassan, Muzhda Haydar Saber, Ahmed Hameed Al-Dabhawi, Goran Noori Saleh, Talal Salem Al-Qaisi
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引用次数: 0

摘要

大戟(Euphorbia Altissima)是一种传统的草药,用于治疗许多与炎症相关的健康疾病,包括皮肤问题。本研究在大鼠切除模型中评估了大黄蜂(Euphorbia Altissima, MEEA)甲醇提取物的植物化学成分、急性毒性和伤口愈合作用。24只Sprague Dawley大鼠颈部背侧切除,分别给予阳性对照、局内凝胶或MEEA(250和500 mg/kg)治疗,为期两周。采用植物化学、组织病理学和免疫组织化学分析来描述伤口愈合潜力。植物化学分析结果表明,黄酮类化合物的总酚含量(283.10 mg GAE/g)和总黄酮含量(211.5 mg芦丁/g)均有所增加。补充MEEA没有造成任何可观察到的毒性损害,在摄入高达5 g/kg的大鼠中没有任何发病率或死亡率。与阳性对照大鼠相比,MEEA处理以剂量依赖的方式导致伤口更近、更小的加速。组织学分析显示,与阳性对照相比,经MEEA治疗的皮肤组织中炎症细胞减少,成纤维细胞增多,胶原沉积增多。MEEA处理引起组织免疫组织化学(Bax减少,HSP 70增加)蛋白和血清炎症(TNF-α, IL-6和IL-10增加)化学物质的显著调节,有助于更快的伤口恢复。此外,经MEEA处理的皮肤组织中羟脯氨酸和内源性抗氧化剂含量较高,MDA含量较低。这项研究阐明了MEEA是一种有效的伤口愈合剂,支持其民间用途,并为未来的探索提供科学证据,包括分子鉴定和分离作为治疗配方的可行来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Therapeutic effects of Euphorbia altissima on excisional wound model: role of apoptosis, oxidative stress, and inflammatory molecules.

Euphorbia Altissima (E. altissima) is a traditional medicinal herb used for many inflammatory-related health disorders, including skin problems. The study estimates the phytochemicals, acute toxicity, and wound-healing action of methanolic extracts of Euphorbia Altissima (MEEA) in excisional rat models. Twenty-four Sprague Dawley rats were excised at their dorsal neck and were addressed with either positive control, intrasite gel, or MEEA (250 and 500 mg/kg) for a two-week trial. Phytochemical, histopathological, and immunohistochemical assays were employed to depict wound healing potentials. Phytochemical profiling showed the increased total phenolic (283.10 mg GAE/g) and flavonoid contents (211.5 mg rutin/g) in methanolic extracts of E. altissima. MEEA supplementation did not cause any observable toxic damage, with the absence of any morbidity or mortality in rats ingested with up to 5 g/kg. MEEA addressing resulted in the acceleration indicated by closer and smaller wounds in a dose-dependent manner compared to positive control rats. Histological analysis demonstrated fewer inflammatory cells, more fibroblasts, and higher collagen deposition in skin tissues treated with MEEA than in positive controls. MEEA addressing caused significant modulation of tissue immunohistochemical (decreased Bax and increased HSP 70) proteins and serum inflammatory (reduced TNF-α, IL-6, and magnified IL-10) chemicals, aiding in faster wound recovery. Moreover, hydroxyproline and endogenous antioxidants were higher, and MDA contents were lower in skin tissues addressed with MEEA. This study elucidates MEEA as an efficient wound healer, supporting its folkloric use and providing scientific evidence for future exploration, including molecular identification and isolation as viable sources of therapeutic formulation.

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来源期刊
Journal of Molecular Histology
Journal of Molecular Histology 生物-细胞生物学
CiteScore
5.90
自引率
0.00%
发文量
68
审稿时长
1 months
期刊介绍: The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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