Journal of Molecular Histology最新文献

{"title":"Effects of hypervitaminosis C and D on spermatogenesis through CDKN1B modulation in adult male mice: biochemical and immunohistochemical studies.","authors":"Karima Nasraldin, Ayman A Diab, Amira S AbdElkhalek, Selvia N Talaat, Salma Hossameldin, Fady S Tawfik, Nada K Ghaith, Ahmed Said","doi":"10.1007/s10735-025-10483-7","DOIUrl":"10.1007/s10735-025-10483-7","url":null,"abstract":"","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 4","pages":"204"},"PeriodicalIF":2.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The metformin administration on pancreatic tissue damage in metastatic rat prostate cancer and STZ induced diabetes model.","authors":"Pınar Koroglu, Onur Ertik, Omur Karabulut Bulan, R Yanardag","doi":"10.1007/s10735-025-10478-4","DOIUrl":"10.1007/s10735-025-10478-4","url":null,"abstract":"<p><p>Cancer is the major cause of mortality in most nations, and a severe health problem worldwide. According to epidemiological studies, diabetes shows an increased risk for a variety of cancers. Metformin is a semisynthetic biguanide produced from the French lilac plant. The effects of metformin on pancreatic tissue damage in cancer and diabetic rat models were evaluated histologically and biochemically in the present study. The diabetes model was induced in Copenhagen rats using a single dose of streptozotocin, while prostate cancer was induced through subcutaneous inoculation of MAT-LyLu cells into the animals. Metformin was administered by gavage daily after inoculation of the Mat-Lylu cells. Histological evaluation showed moderate to severe damage to the pancreas following diabetes and cancer process. Administration of metformin reversed these effects showing a beneficial effect of metformin. Metformin treatment can be considered an adjuvant candidate for pancreas tissue in diabetes, prostate cancer and cancer therapy related damage. Metformin alleviates diabetes and cancer induced pancreatic cytotoxicity by regulating oxidative stress and antioxidant capacity. More research will be needed to explore the metformin effect.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 4","pages":"201"},"PeriodicalIF":2.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The oncogenic role of TRIM56 in pancreatic cancer via the TRAF6/NF-kB axis.","authors":"Chenglin Jiang, Haitao Li, Dazhou Li, Xinxin Li, Shengzheng Luo, Liusheng You","doi":"10.1007/s10735-025-10429-z","DOIUrl":"10.1007/s10735-025-10429-z","url":null,"abstract":"<p><p>Pancreatic adenocarcinoma remains one of the most challenging malignancies in oncology, characterized by its exceptionally high mortality rate. TRIM56 (Tripartite Motif Containing 56) has demonstrated significant roles in various cancer types, yet its biological functions in pancreatic cancer remain largely unexplored. This study investigates the expression patterns and functional significance of TRIM56 in pancreatic tumor development and progression. Gene Expression Profiling Interactive Analysis (GEPIA) and Kaplan-Meier (K-M) plotter analyses were conducted on The Cancer Genome Atlas-Pancreatic Adenocarcinoma (TCGA-PRAD) datasets. We measured TRIM56 levels in pancreatic tumor samples and cell cultures through quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western blotting. Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EDU), and Transwell assays were employed to evaluate how TRIM56 knockdown affected cellular proliferation, migration, and invasion capabilities. Overexpression in normal pancreatic duct epithelial (HPDE) cells was also performed to assess oncogenic potential of TRIM56. Animal studies utilized both subcutaneous xenograft and liver metastasis models in nude mice to evaluate the impacts of TRIM56 knockdown on tumor growth and metastatic potential. Pancreatic cancer tissues and cell lines exhibited significantly elevated TRIM56 expression. Higher TRIM56 expression was linked to shorter survival times in patients with pancreatic cancer. Reducing TRIM56 expression in BxPC-3 and PANC-1 cells significantly inhibited proliferation, migration, and invasion. At the molecular level, TRIM56 silencing decreased the expression of Tumor Necrosis Factor Receptor-Associated Factor 6 (TRAF6) and inhibited the activation of Nuclear Factor kappa B (NF-κB), suggesting their role in TRIM56's cancer-promoting effects. Overexpression of TRIM56 in normal pancreatic epithelial cells promoted cellular aggressiveness. In xenograft models, TRIM56 knockdown resulted in reduced tumor volume and diminished liver metastases. TRIM56 promotes pancreatic cancer progression through activation of the TRAF6/NF-κB signaling pathway. Targeting TRIM56 represents a promising therapeutic strategy for pancreatic cancer treatment.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 4","pages":"205"},"PeriodicalIF":2.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the anticancer potential of Bergenia ciliata conjugated silk fibroin nanoparticles through histopathological and biomarkers study.","authors":"Faiza, Shaukat Ali, Muhammad Summer, Faiza Sharif","doi":"10.1007/s10735-025-10484-6","DOIUrl":"10.1007/s10735-025-10484-6","url":null,"abstract":"<p><p>The present research has aimed to formulate fibroin nanoparticles (FNPs) and Bergenia ciliata-loaded fibroin nanoparticles (BCFNPs) to investigate their antitumor activity against breast cancer in mice. The prepared FNPs and BCFNPs characterized by Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Dynamic light scattering (DLS), Zeta potential measurement, Fourier-transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD). UV-Vis spectroscopy revealed characteristic peak at 282 nm for FNPs and 285 nm for BCFNPs, indicating the successful development of fibroin nanoparticles (FNPs) and Bergenia ciliata-loaded fibroin nanoparticles (BCFNPs). FT-IR analysis identified characteristic absorption bands in the FNPs spectrum at 3350 cm^-1, 1654 cm^-1, 1587 cm^-1, and 1087 cm^-1, indicating the preserved secondary structure of fibroin. In the BCFNPs spectrum, peak shifts and intensity variations were observed at 3338 cm^-1 and 1180 cm^-1 due to the loading of the bioactive compound, indicating successful incorporation of B. ciliata. The DLS analysis confirmed that the FNPs were within the nanometer size range, while the zeta potential measurements indicated that FNPs and BCFNPs have slightly negative surface charge. The SEM analysis assessed the shapes of nanoparticles ranging from round, triangular, and hexagonal shapes and XRD peaks at 2ϴ (20-80) confirmed crystalline nature of FNPs and BCFNPs. In the present study, we established a mice model (Swiss albino) of breast cancer induced by CdCl₂ and treated with tamoxifen, Bergenia ciliata, FNP, and BCFNP to access their antitumor activity. During breast cancer induction, CdCl2 treated groups experienced weight loss, dropping from 30.2 to 18.0 g. After two months, administration of BCFNP significantly inversed the alteration of body weight. At the end of the trial, levels of blood serum biomarkers analyzed. All treatment groups showed better results but BCFNPs treated group exhibited significant reduction (P < 0.0001) in TNF-α (31.7 ± 1.4 pg/ml), IL-6 (20.2 ± 0.9 pg/mL), IL-10 (25.4 ± 1.9 pg/mL), LDH (481.0 ± 7.5 μmol/ml), ASAT (179.0 ± 7.3 μmol/ml), ALAT (532.8 ± 13.4 μmol/ml), and ALP (164.8 ± 5.9 μmol/ml) along with reduced tumor volume. Moreover, Significant (P < 0.0001) improvements in GSH and MDA (248.6 ± 7.9 μmol/ml) serum biomarkers also found. Histological analysis of the BCFNPs treated group revealed a significant reduction in ductal carcinoma. In conclusion, Bergenia ciliata loaded fibroin nanoparticles have potent potential to treat tumors by targeted drug delivery.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 4","pages":"203"},"PeriodicalIF":2.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of renal COX-2 distribution of prenatally administered diclofenac sodium in postnatal rats using stereological and immunohistochemical methods.","authors":"Murat Seven, Murat Çetin Rağbetli̇, Remzi Erten","doi":"10.1007/s10735-025-10480-w","DOIUrl":"10.1007/s10735-025-10480-w","url":null,"abstract":"","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 4","pages":"202"},"PeriodicalIF":2.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sanggenol L inhibits the HMGB1/TLR4/NF-κB signaling pathway to prevent cerebral ischemia-reperfusion damage.","authors":"Xiongyan Mao, Xiongfei Jing, Songchun Liu, Yuchan Zhou, Li Li, Chen Gu, Xiaohua Wang","doi":"10.1007/s10735-025-10472-w","DOIUrl":"10.1007/s10735-025-10472-w","url":null,"abstract":"<p><p>Cerebral ischemia/reperfusion damage (CI/RI) is a recurring pathogenic process in post-ischemic stroke. Sanggenol L (SL) is a flavonoid of Morus alba root bark, which exhibits anticancer, neuroprotective, anti-inflammatory, and antioxidant properties. The signaling pathways involved underlying mechanisms of SL on CI/RI are not exploited. To examine the action of SL on CI/R-instigated brain injury through the inflammatory network of high mobility group box 1 (HMGB1), toll-like receptor 4 (TLR4), and nuclear factor kappa B (NF-κB) in rats was explored. Rats were separated into 5 sets: control, I/R-induced, I/R + Edaravone (ED, 6 mg/kg bw), I/R + SL (10 mg/kg), I/R + SL (20 mg/kg bw). CI/RI was induced by implanting a thread into the middle cerebral artery occlusion (MCAO) model. Cerebral damages were evaluated by using neurological deficit score, brain edema, brain infarct volume, histopathology, apoptosis, and oxidative stress in rats. SL inhibited cytokines, lipid peroxidation, and apoptosis while improving antioxidant status in MCAO rats. Furthermore, SL therapy reduced I/R-induced brain dysfunction and neuroinflammation by suppressing the HMGB1/TLR4/NF-κB pathways. SL could be a potential strong inhibitor of the CI/RI by suppression of the HMGB1/TLR4/NF-κB signaling pathway. SL's potential anti-inflammatory and antioxidant properties suggest it may be a promising therapeutic agent for CI/RI.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 4","pages":"199"},"PeriodicalIF":2.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gender differences in expression and localization of the apelin receptor in mice skin.","authors":"Milirani Das, Rahul Kumar, Vikash Kumar, Guruswami Gurusubramanian, Vikas Kumar Roy","doi":"10.1007/s10735-025-10492-6","DOIUrl":"https://doi.org/10.1007/s10735-025-10492-6","url":null,"abstract":"<p><p>The male and female mouse skin differs in their structure. Leptin, adiponectin, resistin and omentin are adipokines, which expresses in the skin. Apelin is an adipokines, which binds to a its receptor (APJ) and elicits various biological functions in the different tissues. However, the expression of apelin and APJ has not been shown in the mice skin. Since the male female differs, thus, it is hypothesized that apelin and APJ expression might also show gender-wise differences. Our results showed that apelin and APJ are expressed in the epidermal layer, hair follicle and sebaceous glands of male and female skin. This result suggests that apelin-mediated signalling could be different in the male and female skin. Hair follicle and sebaceous gland in the female skin also showed an increased abundance of apelin, which further suggests the role of apelin, in hair growth and sebaceous gland functions. Apelin-mediated signaling in the skin might be gender-dependent. Elevated expression of ERs in female skin coincides with increased apelin and APJ and decreased apelin and APJ expression in male skin coincides with the decreased androgen receptor. Thus, it may be suggested that sex hormonal signaling could be reason for gender wise difference in apelin and APJ expression in skin. Furthermore, apelin signaling would be important for both male and female skin physiological processes, however, apelin signaling could be pronounced in the female skin compared to the male skin. The exact role of gender base apelin signaling needs further investigation in relation proliferation.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 4","pages":"197"},"PeriodicalIF":2.9,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EIF4A3-Mediated downregulation of circPTEN promotes hepatocellular carcinoma progression through the miR-1289/RBM38 Axis.","authors":"Shuo Yu, Min Wang, Feng Peng, Hang Zhang, Renyi Qin, Chengjian Shi","doi":"10.1007/s10735-025-10473-9","DOIUrl":"https://doi.org/10.1007/s10735-025-10473-9","url":null,"abstract":"<p><p>Hepatocellular carcinoma (HCC) remains a leading cause of cancer mortality worldwide. Our research endeavored to delineate the role of circPTEN and to assess its potential as a prognostic biomarker in HCC. CircPTEN expression was quantified in HCC cells and clinical specimens using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The effects of circPTEN overexpression on cellular activities were evaluated through Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EDU), and Transwell assays. The impact of circPTEN overexpression on HCC tumorigenesis and metastasis was also assessed in xenograft mouse models. Kaplan-Meier survival analysis was conducted to assess circPTEN's prognostic value, while additional experiments were conducted to examine the circPTEN-microRNA-1289 (miR-1289) interaction and Eukaryotic Initiation Factor 4A3 (EIF4A3) regulatory effect on circPTEN expression. Our investigations revealed significantly reduced circPTEN expression in HCC, with lower levels correlating with poorer patient outcomes. In vitro experiments demonstrated that enhancing circPTEN expression could inhibit both the proliferation and invasiveness of HCC cells. At the molecular level, circPTEN functioned as a microRNA sponge for miR-1289, consequently upregulating RNA Binding Motif Protein 38 (RBM38), a validated tumor suppressor in HCC. Furthermore, EIF4A3 was identified as a negative regulator of circPTEN expression in HCC cells. Nude mouse model experiments corroborated our in vitro results, showing that increased circPTEN expression corresponded with reduced tumorigenesis and metastatic spread. CircPTEN functions as a tumor suppressor in HCC, regulating the miR-1289/RBM38 axis while being negatively regulated by EIF4A3. Restoration of circPTEN expression represents a potential therapeutic strategy for HCC, and circPTEN levels may serve as a candidate prognostic biomarker.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 4","pages":"196"},"PeriodicalIF":2.9,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METTL3 promotes the hypertrophic scar fibrosis via m6A RNA methylation of GRAMD1B mRNA.","authors":"Wanli Guo, Wei Song, Yanbin Zhai, Peiyi Bai, Zhihui Guo, Xiaorui Tian, Peng Duan","doi":"10.1007/s10735-025-10475-7","DOIUrl":"https://doi.org/10.1007/s10735-025-10475-7","url":null,"abstract":"<p><p>Hypertrophic scarring (HS) is a common fibrotic disorder characterized by excessive extracellular matrix deposition and fibroblast proliferation. N6-methyladenosine (m6A) RNA methylation, mediated by METTL3, has emerged as a critical regulator of gene expression and cellular processes. This study investigates the role of METTL3-mediated m6A methylation in hypertrophic scar fibrosis. We found that METTL3 expression and m6A RNA methylation levels were significantly elevated in human hypertrophic scar tissues and TGF-β1-induced human dermal fibroblasts. Silencing METTL3 reduced m6A methylation, impaired fibroblast proliferation, and decreased the mRNA and protein expression of fibrotic markers (ACTA2, Col1a1, Col3a1, and CTGF). Bioinformatics analysis identified GRAMD1B as a key differentially expressed gene in HS tissues. METTL3-mediated m6A methylation enhanced GRAMD1B mRNA stability, promoting its expression. These findings suggest that METTL3 contributes to hypertrophic scar fibrosis by regulating m6A methylation of GRAMD1B mRNA, highlighting METTL3 as a potential therapeutic target for fibrotic disorders.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":"195"},"PeriodicalIF":2.9,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary docosahexaenoic acid impairs prostate cancer progression in TRAMP mice in the early stages of disease through modulation of inflammatory microenvironment.","authors":"Gustavo Matheus Amaro, Alana Della Torre da Silva, Lucas Pagliuca Martins, Sebastião Roberto Taboga, Valéria Helena Alves Cagnon, Rejane Maira Góes","doi":"10.1007/s10735-025-10479-3","DOIUrl":"https://doi.org/10.1007/s10735-025-10479-3","url":null,"abstract":"<p><p>The polyunsaturated fatty acid docosahexaenoic acid (DHA) has impressive anti-inflammatory and pro-resolution properties but its therapeutic use in PCa requires pre-clinical evidence. Here, the transgenic adenocarcinoma of the mouse prostate (TRAMP) was used as a pre-clinical model to assess the effect of DHA intake on the inflammatory microenvironment and proliferative and survival pathways in early- and late-stage disease. TRAMP mice were fed with standard rodent or DHA-enriched diet (DHA-d) for 4 (early stage) or 10 weeks (late stage). The ventral prostate was evaluated using histopathological, immunohistochemical, and western blotting analysis. Serum samples were collected for TNF-α measurement. Histopathological analysis showed that DHA-d delayed the progression of PCa and the development of in situ and well-differentiated carcinoma at both ages. Dietary DHA reduced cell proliferation and increased apoptosis by inhibiting the Akt pathway in late-stage disease and activating ERK1/2 signaling in early-stage disease. DHA-d down-regulated pyroptosis and up-regulated necroptosis in the late stage. The intake of DHA reduced CD4⁺ T-cell and M2-like macrophage and increased CD8⁺ T-cell infiltration only in the late stage. TNF-α systemic level was down-regulated by DHA-d in both periods but the TNF R1 protein level in the prostate diminished only in the late stage. Overall, DHA-d has a protective effect on prostate carcinogenesis of TRAMP mice by stimulating a low inflammatory and anti-tumor feature, reducing the CD4⁺/CD8⁺ ratio, and downregulating the M2-like macrophage profile. Such immunomodulatory effects suggest a protective action of dietary DHA in the early stages of PCa.</p>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":"194"},"PeriodicalIF":2.9,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}