Fermentation of Panax ginseng C.A. Meyer extract with lactic acid bacteria exerts hepatoprotective effects via activating the NRF2 pathway

IF 2.2 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2025-10-14 DOI:10.1007/s10735-025-10586-1

Jia-Jun Liang, Hong-Xia Liu, Jian-Gang Yan, Guo Xie, Wen-Li Liu, Jia-Hui Lin, Xiao-Min Li, Xin-Liang Mao

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Abstract

The specific hepatoprotective mechanism of Fermented Panax ginseng C.A. Meyer extract (FGE) has not yet been fully elucidated. In this study, an AAPH-induced liver injury model was established to investigate the hepatoprotective effects of FGE. The hepatoprotective effects of FGE may involve the mediation of the NRF2 pathway by rare ginsenosides such as 20(R)-Rh1, 20(S)-Rg2, Rg5, 20(R)-Rg2, and 20(S)-Rh1. The results showed that FGE effectively improved pathological damage and liver dysfunction, increased SOD and GPx expression in the liver, and inhibited excessive ROS generation both in vivo and in vitro. Mechanistically, FGE regulated the mRNA and protein expression of KEAP1, HO-1, and NQO1, while enhancing NRF2 protein expression and promoting its nuclear translocation. In conclusion, the NRF2 signaling pathway activated by FGE may inhibit AAPH-induced oxidative stress and alleviate the resulting liver injury.

Graphical abstract

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乳酸菌发酵人参提取物通过激活NRF2通路发挥保肝作用

发酵人参提取物（FGE）的具体保肝机制尚未完全阐明。本研究通过建立aaph诱导的肝损伤模型，探讨FGE对肝脏的保护作用。FGE的肝保护作用可能通过20(R)-Rh1、20(S)-Rg2、Rg5、20(R)-Rg2和20(S)-Rh1等稀有人参皂苷介导NRF2通路。结果表明，FGE在体内和体外均能有效改善病理损伤和肝功能障碍，提高肝脏SOD和GPx的表达，抑制过量ROS的产生。机制上，FGE调节KEAP1、HO-1和NQO1 mRNA和蛋白表达，同时增强NRF2蛋白表达，促进其核易位。综上所述，FGE激活的NRF2信号通路可能抑制aaph诱导的氧化应激，减轻由此引起的肝损伤。图形抽象

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.