Tracking toxicity of copper oxide nanoparticles (CuO NPs) on PGPR Bacillus megaterium in the presence of melatonin

IF 5.1 2区 环境科学与生态学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Gyan Datta Tripathi, Zoya Javed, Kavya Dashora
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Abstract

The application and accumulation of CuO NPs in the soil may adversely affect the soil microbial community and limit its functional properties. Thus, researchers have tried to mitigate the toxicity of CuO NPs by investigating their underlying mechanisms. However, a detailed mechanism has not been adequately reported to date. Elevated levels of reactive oxygen species (ROS) and contact-mode toxicity are possible reasons for the toxicity of CuO NPs. Herein, we propose the use of melatonin as a mitigating agent for CuO-mediated toxicity due to its ROS neutralization potential. The present study showed that melatonin can reduce the toxicity of CuO NPs on isolated PGPR B. megaterium. Disc diffusion results demonstrated a decrease in the zone of clearance after adding melatonin at a particular concentration, which was significantly observed with CuO NPs (0.1 mg mL−1 and 1 mg mL−1). Changes in the morphology and the cell envelope during the mid-log phase were observed via TEM analysis. Furthermore, the application of melatonin in the IAA production medium inhibited the reduction in IAA production, which was observed earlier due to the presence of CuO NPs in the medium. Additionally, the DCFH staining and live-dead analysis confirmed the potential of melatonin in reducing the toxicity of CuO NPs at specific concentrations (20 and 50 μM).

Abstract Image

在褪黑激素存在下,氧化铜纳米颗粒(CuO NPs)对巨芽孢杆菌的毒性追踪
CuO NPs在土壤中的施用和积累可能对土壤微生物群落产生不利影响,限制其功能特性。因此,研究人员试图通过研究其潜在机制来减轻CuO NPs的毒性。然而,迄今为止尚未有详细的机制报告。活性氧(ROS)水平升高和接触毒性可能是CuO NPs毒性的原因。在此,我们建议使用褪黑激素作为cuo介导的毒性的缓解剂,因为它具有ROS中和的潜力。本研究表明,褪黑素可降低CuO NPs对离体巨芽孢杆菌的毒性。椎间盘扩散结果显示,加入特定浓度的褪黑激素后,清除区减少,这在CuO NPs (0.1 mg mL - 1和1 mg mL - 1)中显著观察到。透射电镜观察了中对数期细胞形态和包膜的变化。此外,在IAA产生培养基中应用褪黑素抑制了IAA产生的减少,这是由于培养基中存在CuO NPs而早期观察到的。此外,DCFH染色和活体分析证实了褪黑素在特定浓度(20和50 μM)下降低CuO NPs毒性的潜力。
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来源期刊
Environmental Science: Nano
Environmental Science: Nano CHEMISTRY, MULTIDISCIPLINARY-ENVIRONMENTAL SCIENCES
CiteScore
12.20
自引率
5.50%
发文量
290
审稿时长
2.1 months
期刊介绍: Environmental Science: Nano serves as a comprehensive and high-impact peer-reviewed source of information on the design and demonstration of engineered nanomaterials for environment-based applications. It also covers the interactions between engineered, natural, and incidental nanomaterials with biological and environmental systems. This scope includes, but is not limited to, the following topic areas: Novel nanomaterial-based applications for water, air, soil, food, and energy sustainability Nanomaterial interactions with biological systems and nanotoxicology Environmental fate, reactivity, and transformations of nanoscale materials Nanoscale processes in the environment Sustainable nanotechnology including rational nanomaterial design, life cycle assessment, risk/benefit analysis
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