Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry最新文献

Sea Anemone Heteractis magnifica Toxin Hct-S3 Suppresses the Migration of Solid Ehrlich Adenocarcinoma Cells Inoculated into BALB/C Mice

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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2025-03-18 DOI: 10.1134/S1990750824601206

A. P. Pavlenko, A. A. Klimovich, E. A. Yurchenko, A. N. Kvetkina, E. V. Leychenko

{"title":"Sea Anemone Heteractis magnifica Toxin Hct-S3 Suppresses the Migration of Solid Ehrlich Adenocarcinoma Cells Inoculated into BALB/C Mice","authors":"A. P. Pavlenko, A. A. Klimovich, E. A. Yurchenko, A. N. Kvetkina, E. V. Leychenko","doi":"10.1134/S1990750824601206","DOIUrl":"10.1134/S1990750824601206","url":null,"abstract":"Malignant tumors represent a serious problem for humanity due to their widespread distribution and severe posttherapeutic consequences. The formation of metastases is the main criterion for tumor malignancy. A search for new compounds with antimetastatic activity remains an urgent problem. In this work, the antitumor activity of a cytolytic toxin Hct-S3 оf the sea anemone Heteractis magnifica was studied in an in vivo model of solid Ehrlich adenocarcinoma. It was established that intraperitoneal administration of Hct-S3 at doses of 0.01 and 0.02 mg/kg has hardly any effect on the tumor growth rate but prevents its metastasis from the primary tumor localization to other tissues. A significant decrease in the proliferative capacity of tumor cells was observed in the groups of mice treated with 0.01 and 0.02 mg/kg Hct-S3: the area of the tumor fluorescence zone decreased by 40 and 48%, and the index of integrated fluorescence density increased by 134 and 146%, respectively, as compared with animals without therapy. The obtained results confirm a high antiproliferative and antimetastatic potential of Hct-S3, which allows it to be considered as a promising compound for creating antitumor drugs.","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S99 - S105"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The Role of MicroRNA in the Development of Type 2 Diabetes Mellitus 微RNA在2型糖尿病发病过程中的作用

IF 0.6

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2025-03-18 DOI: 10.1134/S1990750824600869

A. V. Krasnobaeva, V. A. Dugina

引用次数: 0

Peptide TRPV1 Channel Blocker, HCRG21, Effectively Suppresses Inflammation in a Calcipotriol-Induced Allergic Dermatitis Model

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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2025-03-18 DOI: 10.1134/S1990750824601127

A. A. Klimovich, A. N. Kvetkina, Yu. V. Deryavko, N. A. Priymenko, E. V. Leychenko

{"title":"Peptide TRPV1 Channel Blocker, HCRG21, Effectively Suppresses Inflammation in a Calcipotriol-Induced Allergic Dermatitis Model","authors":"A. A. Klimovich, A. N. Kvetkina, Yu. V. Deryavko, N. A. Priymenko, E. V. Leychenko","doi":"10.1134/S1990750824601127","DOIUrl":"10.1134/S1990750824601127","url":null,"abstract":"As a result of application of hydrogels with 0.05 and 0.005% HCRG21 calcipotriol-induced inflammatory swelling and flaking of the auricle were significantly reduced. As a result of application of hydrogels with 0.05 and 0.005% HCRG21 calcipotriol-induced inflammatory swelling and flaking of the auricle were significantly reduced. Treatment with HCRG21 normalized peripheral blood parameters in mice, including total white blood cell count and basophil percentage. In addition, treatment with gels containing HCRG21 resulted in a decrease in the level of proinflammatory cytokines: interleukin-23 (IL-23) and macrophage-derived chemokine (MDC) by 1.5 and 2 times, respectively, compared to the control group of animals. According to the results of histological analysis of the auricle of mice, treatment with peptide-containing gels enhanced the regeneration of the affected skin area, reducing hyperkeratosis, hyperplasia of the epidermis and dermis, and tissue hypervascularization. Thus, the discovered anti-inflammatory and regenerative effect of HCRG21 in the calcipotriol-induced allergic dermatitis model determines the potential of the peptide as a dermatotropic drug.","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S106 - S113"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conference “Human Biochemistry” Dedicated to the 100th Birth Anniversary of Temirbolat Berezov

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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2025-03-18 DOI: 10.1134/S1990750824601462

V. S. Pokrovsky

引用次数: 0

Evaluation of Phenylalanine Substitution on the Antimicrobial Properties of Histatin 8 against Wild-Type and Antibiotic-Resistant Pseudomonas aeruginosa 苯丙氨酸替代物对组蛋白 8 对抗野生型和抗生素耐药铜绿假单胞菌抗菌特性的评估

IF 0.6

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2025-03-18 DOI: 10.1134/S1990750824600353

Nedrick T. Distor, Leslie Michelle M. Dalmacio

{"title":"Evaluation of Phenylalanine Substitution on the Antimicrobial Properties of Histatin 8 against Wild-Type and Antibiotic-Resistant Pseudomonas aeruginosa","authors":"Nedrick T. Distor,  Leslie Michelle M. Dalmacio","doi":"10.1134/S1990750824600353","DOIUrl":"10.1134/S1990750824600353","url":null,"abstract":"The unregulated and inappropriate use of antibiotics triggered the rapid spread of antimicrobial resistance in pathogenic microorganisms such as Pseudomonas aeruginosa. Antimicrobial peptides (AMPs) require investigations of their remarkable bioactivities and unique microbicidal mechanisms as potential replacement treatments for conventional antibiotics. Specifically, a naturally occurring antimicrobial dodecapeptide named Histatin 8 (HS8), from the Histatin family, has weak to moderate antimicrobial activities but is a promising bioactive molecule. In this study, three well-represented HS8-derived peptides with potential in silico bioactive propensities namely HS8-1 (Tyr12Phe), HS8-2 (Ser8Phe and Tyr12Phe), and HS8-3 (Gly11Phe) were explored. Results showed that HS8-1 was a significantly more active AMP than HS8-2 and HS8-3, displaying 3.0-fold and 3.56-fold decrease in minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, respectively, against wild-type P. aeruginosa. Furthermore, HS8-1 and HS8-2 displayed the lowest MIC and MBC values against the antibiotic-resistant P. aeruginosa. Both HS8-2 and HS8-1 are the more active peptides showing inhibitory activities against wild-type and antibiotic-resistant P. aeruginosa biofilms in vitro, respectively. HS8-1 and HS8-2, at their MICs, can both primarily induce surface roughening and corrugation suggesting possible disruptive effects on the P. aeruginosa membrane reflecting antimicrobial action. HS8-1 lyses human red blood cells (RBCs) in vitro at concentrations 250 µg/mL and below, suggesting it to be the least hemolytic peptide compared to HS8-2 and HS8-3 which strongly lyse RBCs at relatively lower concentrations. Overall, the results imply that the HS8-derived peptide bioactivities can be enhanced by site-specific phenylalanine (Phe) substitutions against P. aeruginosa.","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S26 - S43"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Antiglycating and Antioxidant Properties of Atranorin Isolated from Lichen Cladonia rangiferina

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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2025-03-18 DOI: 10.1134/S1990750824700033

I. A. Prokopiev, O. S. Shemchuk, U. A. Kremenetskaya, O. E. Molchanov, D. N. Maistrenko, K. N. Semenov, V. V. Sharoyko

引用次数: 0

The Influence of Vitamins D and K on Some Biochemical, Cytochemical, and Clinical Blood Parameters In Vivo with Separate and Combined Administration

IF 0.6

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2025-03-18 DOI: 10.1134/S1990750824601176

O. V. Popova, A. V. Mikhailov, G. P. Drobot, N. V. Turmukhametova, V. P. Manaeva, T. V. Ivanova, Y. A. Kupsoltseva, S. B. Mustafaeva, R. I. Bulgariev

{"title":"The Influence of Vitamins D and K on Some Biochemical, Cytochemical, and Clinical Blood Parameters In Vivo with Separate and Combined Administration","authors":"O. V. Popova, A. V. Mikhailov, G. P. Drobot, N. V. Turmukhametova, V. P. Manaeva, T. V. Ivanova, Y. A. Kupsoltseva, S. B. Mustafaeva, R. I. Bulgariev","doi":"10.1134/S1990750824601176","DOIUrl":"10.1134/S1990750824601176","url":null,"abstract":"A comprehensive study was conducted on the effect of vitamins D and K on some indicators of lipid peroxidation and the overall antioxidant activity of blood plasma in rats of two age groups. Differences in the studied parameters were revealed both in terms of age and under the influence of administered vitamins. An antioxidant effect of vitamin D is noted in adult animals, which is expressed in a significant decrease in the level of malondialdehyde (MDA) in blood plasma. In young people, a similar effect was found with the combined action of vitamins D and K. The content of lipids, glycogen, and cationic proteins in blood neutrophils of animals receiving vitamins D and K separately and together was analyzed. Significant changes in the content of cationic proteins were revealed when vitamin K was administered to adult animals. When analyzing the leukocyte formula, vitamin K and a complex of vitamins D and K showed the greatest effect, reflected in a change in the proportion of monocytes and lymphocytes. A negative correlation was shown between the lipid content in blood neutrophils and the level of one of the lipid peroxidation products, MDA, in blood plasma.","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S82 - S90"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The Effect of Kisspeptin Preparations on the Expression of CRH, GRH, GNRHR1, and OXT Genes in the Brain of Danio rerio after Social Isolation

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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2025-03-18 DOI: 10.1134/S199075082460122X

A. P. Perova, V. A. Golts, A. V. Lizunov, E. A. Sekste, E. R. Bychkov, A. A. Bairamov, A. A. Lebedev, P. D. Shabanov

引用次数: 0

Molecular Genetic Determinants of Pregnancy Pathology

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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2025-03-18 DOI: 10.1134/S1990750824600882

A. B. Kazumova

{"title":"Molecular Genetic Determinants of Pregnancy Pathology","authors":"A. B. Kazumova","doi":"10.1134/S1990750824600882","DOIUrl":"10.1134/S1990750824600882","url":null,"abstract":"In the modern world, scientists often discuss problems associated with complications of pregnancy and childbirth in women. The study of cases of repeated fetal loss, stillbirth, intrauterine growth retardation and preeclampsia leads to the discovery of new aspects of this pathology. Of particular interest is Upshaw−Schulman syndrome, a rare congenital form of thrombotic thrombocytopenic purpura caused by mutations in the ADAMTS13 gene. This gene encodes a metalloproteinase capable of cleaving von Willebrand factor, which is important for blood clotting processes. A review of modern Russian and foreign, primarily English-language, literature was conducted on methods of diagnosis and treatment of congenital thrombocytopenic purpura as well as the prevention of complications of pregnancy and childbirth. This review highlights the main mechanisms of development and progression of the syndrome, current directions of management of women with Upshaw−Schulman syndrome, and methods of therapy of associated reproductive failure, and also presents modern groups of pharmacological drugs of choice. Pregnancy with Upshaw−Schulman syndrome is becoming a major focus of research in obstetrics, and it is important to conduct further high-quality association studies to develop innovative therapeutic options and options in the future.","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S44 - S50"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Study on the Mechanisms of Antifibrotic Action of Soluble Proteins of Human Mesenchymal Stromal Cell Secretome

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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2025-03-18 DOI: 10.1134/S1990750824601085

M. Kulebyakina, D. Butuzova, O. Klychnikov, Yu. Strogov, N. Basalova, A. Efimenko

{"title":"Study on the Mechanisms of Antifibrotic Action of Soluble Proteins of Human Mesenchymal Stromal Cell Secretome","authors":"M. Kulebyakina, D. Butuzova, O. Klychnikov, Yu. Strogov, N. Basalova, A. Efimenko","doi":"10.1134/S1990750824601085","DOIUrl":"10.1134/S1990750824601085","url":null,"abstract":"Multipotent mesenchymal stromal cells (MSCs) are key regulators of tissue healing after injury. Acting in a paracrine manner, MSCs promote tissue regeneration and prevent the development of fibrosis, a pathological condition in which there is excessive replacement of functional tissue with scar tissue. However, the mechanisms of the antifibrotic action of proteins secreted by MSCs have not yet been established. The authors previously showed that the fraction of soluble protein factors (SF) of the MSC secretome is capable of suppressing the key cellular mechanism of fibrosis development, the differentiation of fibroblasts into myofibroblasts, and also established, using proteomic analysis, that this fraction is enriched in proteins capable of regulating the canonical signaling pathways of transforming growth factor beta, Wnt, and Notch in fibroblasts. This study investigated the effect of the SF fraction on these signaling pathways in human skin fibroblasts and found that this fraction suppresses the canonical Wnt signaling pathway in a model of myofibroblast differentiation of fibroblasts. According to the results of proteomic analysis, SF is enriched in the DKK3 protein, which is able to regulate fibroblast differentiation through interaction with components of the canonical Wnt signaling pathway. It was shown that depletion of DKK3 protein in the SF fraction of MSC secretome by immunoprecipitation results in a significant reduction in the ability of the SF fraction to suppress fibroblast differentiation into myofibroblasts in vitro. The obtained data clarify the mechanism of the antifibrotic action of MSC secretome proteins associated with the suppression of differentiation of fibroblasts into myofibroblasts.","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S51 - S60"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0