Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry最新文献

{"title":"Pathogenetic Potential of the Epigenetic Status of Genes of Mendelian Forms of Thoracic Aortic Aneurysms","authors":"A. N. Kucher,&nbsp;Iu. A. Koroleva,&nbsp;M. S. Nazarenko","doi":"10.1134/S1990750824600183","DOIUrl":"10.1134/S1990750824600183","url":null,"abstract":"<p>Rare pathogenic variants in genes whose protein products form the structure of the extracellular matrix, regulate smooth muscle cell function, or belong to the TGFβ/SMAD signaling pathway are detected in 20–30% of thoracic aortic aneurysm (TAA) cases. In addition to the structural changes, epigenetic events associated with these genes may influence the risk of aortic aneurysm. This review summarizes the published study results on the functional significance of epigenetic modifications in the genes of Mendelian forms of TAA. Most TAA genes are characterized by overlapping localization with genomic noncoding regulatory elements (including genes of microRNAs, long noncoding RNAs, etc.). These genomic regions have not been practically studied in patients with aortic aneurysm, but a number of noncoding RNAs (<i>ACTA2-AS1</i>, <i>TGFB2-AS1</i>, <i>PRKG1-AS1</i>) have been shown to be involved in the regulation of processes pathogenetically significant for aortic aneurysm (proliferation, apoptosis, autophagy, inflammation, endothelial dysfunction) and metabolic pathways (TGFβ/SMAD, Wnt/β-catenin). In the regions of TAA genes, the changes in DNA methylation are also recorded both in the presence of risk factors (structural features of the aorta, blood flow patterns) and in the development of aortic aneurysm. Various epigenetic events may also exert the pathogenic effects of aortic aneurysm-associated genetic variants localized in TAA genes. Thus, despite the fact that the long noncoding RNA and microRNA genes located in the TAA gene region and various epigenetic events (including histone modifications and DNA methylation) have not been sufficiently studied in this aortopathy, the information provided in scientific publications allows us to consider these epigenetic markers as promising regulators of certain pathogenetic processes in the development of an aortic aneurysm.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"299 - 322"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Procarcinogenic Characteristics of Tryptophan Metabolism in Obese Patients","authors":"O. P. Shatova,&nbsp;A. A. Zabolotneva,&nbsp;A. V. Shestopalov","doi":"10.1134/S1990750824600766","DOIUrl":"10.1134/S1990750824600766","url":null,"abstract":"<p>Tryptophan is an essential amino acid, the catabolism of which occurs in the human body along three main pathways: kynurenine, serotonin, and indole. At the same time, a significant part of tryptophan metabolites is formed as a result of the enzymatic activity of the intestinal microbiota. Deviations in tryptophan metabolism, manifested in the form of shifts towards the formation of certain metabolites, are characteristic of some pathological conditions, in particular obesity and malignant neoplasms. It is known that the growth of some types of tumors is associated with excess body weight, but the mechanisms of the procarcinogenic effect of obesity remain not fully understood. Obesity is characterized by the development of systemic inflammation and increased production of proinflammatory cytokines, which increase the expression of the key enzyme of the kynurenine pathway and intensify the formation of immunosuppressive and cytotoxic metabolites: kynurenine and quinolinic acid. Increased stimulation of the kynurenine pathway in the body of obese and cancer patients leads to a decrease in tryptophan itself, which is necessary for the proliferation of immunocompetent cells and a normal immune response. Both obese and cancer patients are characterized by increased levels of quinolinic acid and decreased concentrations of indole-3-propionate in the blood serum. Obese patients are often characterized by a decrease in serotonin in the blood serum, while not only an increase in serotonin production has been established for tumor tissues but also an increase in the expression of various types of serotonin receptors. The existing general trends in changes in tryptophan metabolism indicate the presence of intersection points in the pathogenesis of obesity and tumor development. A literature review was conducted using the PubMed database, primarily using literature sources from 2018–2024.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"287 - 298"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Technology for Applying Nanocoatings to Surgical Meshes and Study of the Functional Properties of the Resulting Nanofilms","authors":"N. M. Abdullaeva,&nbsp;R. M. Ragimov,&nbsp;V. Yu. Khanaliev,&nbsp;I. M. Abdulagatov,&nbsp;M.A. Khamidov","doi":"10.1134/S1990750824700021","DOIUrl":"10.1134/S1990750824700021","url":null,"abstract":"<p>—Today, abdominal hernias of various localizations are diagnosed in approximately 7% of the world’s population. Approximately 20 million hernioplasty operations are performed annually, 85% of which use surgical mesh. The implant itself often becomes the cause of the development of postoperative complications in the form of adhesions, granulomas, fistulas, seromas, abscesses, etc. The study involved surgical meshes with a nanocoating applied using atomic layer deposition (ALD) technology. The aims of the study were the development of a technology for applying a nanocoating to impart antibacterial properties to surgical endoprosthetic meshes and improve their biocompatible properties using the atomic layer deposition method. During the work, a nanocoating was applied to the surface of the polypropylene meshes using the atomic layer deposition (ALD) method to impart antibacterial properties to the implant. As a result of applying the nanofilm, nano-sized roughness was formed on the surface, which prevented the adhesion of microorganisms. At the same time, the main characteristics of the grids themselves did not change. By means of cyclic reactions, using trimethylaluminum, titanium tetrachloride, and vanadium trichloroxide, the first samples of meshes were obtained, which were introduced into the bodies of experimental animals. Complex coating (Al₂O₃ + TiO₂/V₂O₅) demonstrated good integration into the surrounding tissue with minimal inflammatory response. The developed method for obtaining nanocoatings to improve the bactericidal properties of surgical endoprosthesis mesh is simple in industrial implementation, compared to PACVD or CVD, and covers large areas simultaneously, therefore reducing material costs for production and increasing economic efficiency.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"331 - 339"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Search for New Active Substances for the Development of Pharmaceuticals Using Supercritical Fluid Extraction","authors":"A. I. Artemiev,&nbsp;K. M. Demkin,&nbsp;I. V. Kazeev,&nbsp;N. V. Menshutina","doi":"10.1134/S1990750824600511","DOIUrl":"10.1134/S1990750824600511","url":null,"abstract":"<p>The use of supercritical fluids for the extraction of biologically active substances is an extremely urgent task. Extraction in a supercritical fluid environment at temperatures up to 323 K and pressures of 16–20 MPa allows preserving the value of the extracted substances. This article discusses equipment and experimental studies of the process of supercritical fluid extraction of biologically active substances from various plant materials: aralia, ginseng, <i>Tribulus terrestris</i>, and a collection of multiphytoadaptogen. A comparative analysis of the efficiency of supercritical and traditional liquid extraction in a Soxhlet apparatus was carried out. High performance liquid chromatography was used to determine the content of active substances in the obtained extracts. It has been established that the yield of active substances during supercritical fluid extraction is higher than during traditional extraction methods. In addition, the use of ultrasound during supercritical extraction allows increasing the yield of aralosides from aralia.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"323 - 330"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saliva Glucose Determination by Biosensor: Method Comparison to Chemiluminescence Immunoassay","authors":"Rabia Şemsi,&nbsp;Umut Kökbaş,&nbsp;Burak Arslan,&nbsp;Erdal Ergünol,&nbsp;Aylin Sepici Dinçel,&nbsp;Levent Kayrın","doi":"10.1134/S1990750824600286","DOIUrl":"10.1134/S1990750824600286","url":null,"abstract":"<p>The noninvasive sampling materials and methods have garnered great interest among researchers. Saliva is considered the most popular material, and glucose is one of the most commonly used biochemical parameters. This article provides a comparison between the biosensor and autoanalyzer methods for saliva glucose measurement and identifies its potential as a point-of-care testing tool. The experiments were performed using salivary samples from twenty-six adults collected using saliva collection tubes. The swabs were soaked in saliva for 1 min and then centrifuged for 10 min at 1500 <i>g</i>. Glucose concentrations were determined using two methods: one based on an autoanalyzer using chemiluminescence assay and the other based on a biosensor using glucose oxidase enzyme-based amperometric method. A statistically significant correlation was found between the two methods (<i>r</i> = 0.802; <i>p</i> &lt; 0.001). The most remarkable result from the data is that there were no significant differences in saliva glucose measurements between the two methods. This paper presents an innovative biosensor method that can be readily used in practice for rapid results, low cost, and specificity compared to the frequently used autoanalyzer method.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"361 - 367"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Elevated Concentrations of Secretory IgA on the Level of Immunocompetent Cells, Cytokines, Reagins, Circulating Immune Complexes, and C-Reactive Protein in the Blood of Practically Healthy Residents of Murmansk Oblast","authors":"L. V. Gubkina,&nbsp;A. V. Samodova","doi":"10.1134/S1990750824600389","DOIUrl":"10.1134/S1990750824600389","url":null,"abstract":"<p>A total of 104 (77 women and 27 men) practically healthy residents of the city of Apatity, Murmansk oblast, aged 21–55 years were examined. The following indicators were studied in venous blood: the ratio and count of leukocytes, eosinophils, neutrophils, lymphocytes, and monocytes; the lymphocyte count with the phenotypes CD3+, CD4+, CD8+, CD71+, and CD95+ determined by the indirect immunoperoxidase reaction using monoclonal antibodies; the content of cytokines IL-6, IL-10, and TNF-α and immunoglobulin E; the level of secretory immunoglobulin IgA; and the content of C-reactive protein determined by the enzyme immunoassay. It was found that 31.73% of permanent residents of the city of Apatity, Murmansk oblast, had elevated levels of sIgA in their blood. People with increased sIgA concentrations in venous blood had higher counts of leukocytes, predominantly segmented neutrophilic granulocytes, increased concentrations of proinflammatory cytokines (IL-6, TNF-α) and anti-inflammatory (IL-10), reagins, small circulating immune complexes (IgG), and acute phase protein (CRP).</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"340 - 344"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peculiarities of General and Mucosal Immunity in Urban Residents of Murmansk Oblast","authors":"A. V. Samodova,&nbsp;L. V. Gubkina,&nbsp;K. O. Pashinskaya,&nbsp;S. N. Balashova,&nbsp;L. K. Dobrodeeva,&nbsp;A. A. Martynova,&nbsp;E. I. Grigor’eva","doi":"10.1134/S1990750824600407","DOIUrl":"10.1134/S1990750824600407","url":null,"abstract":"<p>The state of general and mucosal immunity was studied in 103 permanently living and working residents of Apatity town, Murmansk oblast. In examined individuals, a high level of deficiency of active neutrophilic phagocytes in the peripheral blood and on mucous membranes of the pharynx, urinary system, and intestine is established. A high frequency of registration of a deficiency of the sorption capacity of epitheliocytes and levels of secretory immunoglobulins A are detected. An increase in the concentration of pathogenic microorganisms on mucous membranes of the pharynx and urinary system and a deficiency of normal microflora on mucous membranes of the intestine are observed. Long life in unfavorable climate conditions is characterized by a high level of deficiency of mature T lymphocytes, T helpers, and T lymphocytes with a transferrin receptor and cells to programmed cell death.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"383 - 389"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143107914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the Expression Landscape of HER2-Negative Breast Cancer under the Influence of Anthracycline-Containing Neoadjuvant Chemotherapy Regimens","authors":"M. K. Ibragimova,&nbsp;M. M. Tsyganov,&nbsp;E. A. Kravtsova,&nbsp;N. V. Litviakov","doi":"10.1134/S1990750824600559","DOIUrl":"10.1134/S1990750824600559","url":null,"abstract":"<p>Currently, several treatment options are available for patients with HER2 breast cancer; however, there is no gold standard first-line treatment. Anthracycline-containing regimens are also considered traditional systemic treatment as preoperative chemotherapy for this category of patients, and they are a controversial decision due to the high degree of toxicity and low complete pathological response rates. That is why new studies are needed to identify biomarkers that predict response to anthracyclines in NAC HER2-BC, both the presence of pCR and a favorable long-term outcome, to personalize preoperative treatment. In this work, changes were studied in the expression profile of breast tumor of the luminal B HER2-negative subtype under the influence of anthracycline-containing NAC regimens in order to identify the groups of potential expression markers: an objective response to the treatment used and predicting the occurrence of hematogenous metastasis.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"368 - 382"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143107915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Synthetic Kisspeptin Derivatives Impair Wound Healing in Breast Adenocarcinoma","authors":"P. M. Kopeikin,&nbsp;E. G. Bogomolova,&nbsp;A. O. Drobintseva,&nbsp;T. S. Kleimenova","doi":"10.1134/S1990750824600560","DOIUrl":"10.1134/S1990750824600560","url":null,"abstract":"<p>Currently, cancer plays an important role in public health. Mortality from cancer in 90% of cases is caused by the development of metastases, which are the clinical result of the invasiveness of the pathology. Among the known metastasis suppressor molecules are the products of the <i>KISS1</i> gene—the metastin protein and its derivatives: kisspeptin-14 (KP14), kisspeptin-13 (KP13) and kisspeptin-10 (KP10). The KISS1/KISS1R system regulates the development and progression of several types of cancer. These molecules are important in the development of new treatment methods and/or as markers in the treatment of cancer, so the biologically active kisspeptin analogues design and synthesis is a promising direction for the development of drugs that prevent the metastases formation. In this study, promising sequences of kisspeptin and its modified analogues—KP-10, KP-10-dAla, KP-6-dAla—were designed. Peptides with a purity of &gt;98% were obtained through chemical synthesis and chromatographic purification. Study of its biological activity on the MCF-7 cell line (breast adenocarcinoma) demonstrated the anti-migration activity of the peptides. The greatest effect was observed with the treatment of 1000 nM KP-10, 10 nM KP-6-dAla, 10 nM KP-10-dAla. It was shown that replacing glycine with D-alanine and shortening the kisspeptin chain led to a slower rate of wound healing of MCF-7 cells compared to control. The potential effectiveness of KP-6-dAla and KP-10-dAla in suppressing metastasis was revealed, thereby making it a promising candidate for the development of anticancer drugs.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"345 - 354"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving the Functional and Antibacterial Properties of an Endoprosthetic Mesh Using Atomic Layer Deposition Technology","authors":"R. M. Ragimov,&nbsp;N. M. Abdullaeva,&nbsp;V. Yu. Khanaliev,&nbsp;M. A. Khamidov,&nbsp;I. M. Abdulagatov","doi":"10.1134/S199075082470001X","DOIUrl":"10.1134/S199075082470001X","url":null,"abstract":"<p>Over the past few decades, abdominal wall hernias of various locations have attracted close attention from surgeons in various countries, including Russians. Approximately 20 million hernioplasty operations are performed annually, at least 85% of which involve the use of surgical meshes. This interest of specialists in hernias is due to serious reasons, first of all, strangulation, which is a life-threatening complication and often leads to death. According to various authors, from 3 to 8% of the population suffer from hernias, and at least 20 million people in the world undergo hernia repair every day, and they are recently increasingly using endoprosthetic meshes from various manufacturers for hernial orifice repair. Often, hernia mesh causes postoperative complications, such as adhesions, wrinkling of the mesh, granulomas, fistulas, seromas, including infection of the implant, leading to the development of abscesses and possibly sepsis. To reduce these complications, various modifications of endoprosthetic meshes and materials are being developed. However, there is not a single mesh at present that would completely satisfy hernia surgeons. Based on the above, the authors set a goal to improve the functional and antibacterial properties of polypropylene endoprosthetic mesh using atomic layer deposition (ALD) nanotechnology. The ALD method consists in that a nanofilm is created on the surface of the endoprosthetic mesh through cyclic reactions using trimethylaluminum, titanium tetrachloride, and vanadium trichloroxide. The authors have obtained the first samples of meshes that were implanted into the bodies of experimental animals. The experimental samples demonstrated good biocompatibility and integration into the surrounding tissue, preventing any inflammatory response. The developed method for improving the functional and antibacterial properties of endoprosthetic meshes is simple in industrial implementation compared to PACVD or CVD and allows covering large areas, therefore, reducing material production costs and increasing the economic efficiency of domestically produced mesh implants and making them competitive in the world market.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"355 - 360"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}