Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry最新文献

{"title":"Effects of Various Poly(A) Tails on Luciferase Expression","authors":"I. Kukushkin,&nbsp;O. Vasileva,&nbsp;D. Kunyk,&nbsp;S. Kolmykov,&nbsp;T. Sokolova,&nbsp;A. Muslimov,&nbsp;I. Terenin,&nbsp;O. Zaborova,&nbsp;A. Kirshina,&nbsp;F. Kolpakov,&nbsp;R. Ivanov,&nbsp;V. Reshetnikov","doi":"10.1134/S1990750824600055","DOIUrl":"10.1134/S1990750824600055","url":null,"abstract":"<p>Synthesis of RNA by in vitro transcription has many applications in the production of vaccines, anticancer and gene therapy medicines. Despite several advantages of mRNA molecules over DNA in terms of transient transgene expression, rapid degradation of RNA is the limiting factor for the efficacy of mRNA-based therapeutics. Translational efficiency and stability of mRNA depends on the sequence of 5′ and 3′ untranslated regions, cap structure, coding sequence (CDS), and the poly(A) tail. So far, the role of the poly(A) tail sequence in mRNA stability and translational efficiency remains poorly investigated. There are few studies evaluating the influence of poly(A) tails containing certain non-A nucleotides or segments of non-A nucleotides on the efficacy of mRNA therapeutics. In our work, we conducted a comprehensive assessment of the effects of mRNA poly(A) tail on luciferase expression in cell lines HEK293 and DC2.4 and in BALB/c mice. Our results showed that mRNAs with a poly(A) tail consisting of 100 adenines separated by a segment of 10 nucleotides (50A-GCAUAUGACU-50A) have the best translational efficiency both in vitro (in cell lines) and in vivo (in BALB/c mice). Nonetheless, the results were inconsistent and depended both on cell lines and on the coding sequence (NanoLuc or firefly luciferase). Thus, effects of a mRNA poly(A) tail on luciferase expression may depend on the sequence of the gene in question and on specific features of the expression of RNA-binding proteins in cell lines.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 3","pages":"263 - 274"},"PeriodicalIF":0.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution of Matrix Metalloproteinases in the Pathogenesis of Cardiovascular Diseases Complicated by Obesity","authors":"S. R. Ledovskikh,&nbsp;Ya. V. Polonskaya,&nbsp;V. S. Shramko","doi":"10.1134/S1990750823600504","DOIUrl":"10.1134/S1990750823600504","url":null,"abstract":"<p>Cardiovascular diseases (CVDs) are one of the world’s main causes of mortality. The role of matrix metalloproteinases (MMPs) as markers of CVD complications has been actively discussed in recent years. It has been proven that a high level of MMPs contributes to the development of unstable plaque, aggravating a patient’s prognosis. Being an independent risk factor, obesity also results in complications of cardiovascular diseases. Studies on the effect an increase in the body mass index and adipose tissue hormones on the level of MMPs have begun to appear. Results from clinical and experimental studies over the last 5 years devoted to studying matrix metalloproteinases are presented here. The role of MMPs in the pathogenesis of atherosclerosis is reflected. MMPs are considered biomarkers of the development of adverse cardiovascular events. Results from studies on the effect obesity has on the level of MMPs are also presented here. A search was performed for articles in the PubMed, Google Scholar, and Elibrary databases for the period from 2018 to 2023 using the keywords metalloproteinases and atherosclerosis, metalloproteinases and obesity, and the connection between atherosclerosis and obesity separately for each type of MMP.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 3","pages":"185 - 191"},"PeriodicalIF":0.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Concentration of Telomeric Repeat Binding Factor 2 (TERF2) in Leukocyte Cells of Patients with Hepatitis","authors":"Endang Purwaningsih,&nbsp;Tripanjiasih Susmiarsih,&nbsp;Yenni Zulhamidah,&nbsp;Achmad Sofwan","doi":"10.1134/S1990750824600092","DOIUrl":"10.1134/S1990750824600092","url":null,"abstract":"<p>Hepatitis is a disease that attacks the organs of the human liver that causes inflammation that makes the liver function. Telomere length and telomerase activity in chronic liver disease and hepatocarcinoma correlate with disease severity. Telomeres are protected by her six shelterin proteins or telosome, namely TERF1/TRF1, TERF2/TRF2, POT1, TPP1, RAP1, and TIN2. TRF2 protein has an important role in regulating the molecular events that maintain telomere integrity. The objective of the research was to determine the concentration of TERF2 in leukocytes in patients with hepatitis. The research design method is descriptive with a case-control study involving 62 subjects. Blood samples were taken from patients with hepatitis from Klinik Pratama Prof. Qomariyah, which amounted to 32 people, including 8 males and 24 females (40–60 years). As a control, employees of YARSI University with the same age range, amounting to 24 people, including 20 men and 4 women. Measurements of TERF2 used the ELISA method. TERF2 protein concentrations in patients with hepatitis leukocytes were significantly higher (<i>p</i> &lt; 0.05) compared to controls. Similarly, TERF2 levels between male patients with male controls and between female patients with female controls also showed significant differences in sufficient concentration (<i>p</i> &lt; 0.05). Instead, the concentrations of TERF2 between patients with hepatitis men and women did not have a difference. The concentration of TERF2 in leucocyte patients with hepatitis showed a concentration higher than in healthy people, both men and women.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 3","pages":"249 - 253"},"PeriodicalIF":0.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Pretreatment with Atorvastatin, Fenofibrate, or Both Drugs in a Mouse Model of Acute Lipemia Induced by the General Lipase Inhibitor Poloxamer 407","authors":"T. A. Korolenko,&nbsp;T. P. Johnston,&nbsp;N. V. Tamkovich,&nbsp;V. A. Vavilin,&nbsp;N. P. Bgatova,&nbsp;I. D. Ivanov,&nbsp;G. S. Russkikh,&nbsp;E. V. Koldysheva,&nbsp;E. C. Korolenko,&nbsp;V. I. Kapustina,&nbsp;S. I. Makarova,&nbsp;N. V. Goncharova,&nbsp;M. M. Gevorgyan,&nbsp;V. M. Loginova","doi":"10.1134/S1990750823600474","DOIUrl":"10.1134/S1990750823600474","url":null,"abstract":"<p>Dyslipidemia is a well-known risk factor for the development of cardiovascular diseases and atherosclerosis. The effects of combined pretreatment with atorvastatin and fenofibrate (Tricor) were studied in a mouse model of acute lipemia induced by a general lipase inhibitor, poloxamer 407 (P-407, 250 mg/kg). This lipemia is characterized by significantly increased serum levels of triglycerides (TG), low-density lipoprotein (LDL) cholesterol, together with decreased concentration of high-density lipoprotein (HDL) cholesterol. Atorvastatin pretreatment had a hypolipidemic effect, decreasing concentrations of LDL cholesterol and increasing HDL cholesterol. Pretreatment of mice with fenofibrate decreased TG level, increasing HDL cholesterol. Combined pretreatment with atorvastatin and fenofibrate decreased TG and total cholesterol. Elevation of the serum cystatin C level was found in control and lipemic mice pretreated with atorvastatin, fenofibrate, or both. Liver expression of lysosomal acid lipase increased in atorvastatin- or/and fenofibrate-pretreated groups of lipemic mice. It was concluded that increased expression of lysosomal acid lipase is related to the removal of lipid droplets from hepatocytes, thus preventing acute lipemia. Lastly, cystatin C may be a “theranostic” biomarker for hypolipidemic drugs.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 3","pages":"214 - 230"},"PeriodicalIF":0.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of Expression Vectors for Efficient Production of Recombinant Proteins in E. coli for the Development of Therapeutic Drugs","authors":"M. V. Zakharova,&nbsp;E. K. Mubarakshina,&nbsp;M. O. Nagornykh","doi":"10.1134/S1990750823600516","DOIUrl":"10.1134/S1990750823600516","url":null,"abstract":"<p>When developing vaccines and immunotherapeutic and enzymatic drugs, it is usually necessary to obtain a functional protein preparation, which can later be used for immunization or analytical studies in the process of creating a drug. Production in <i>E. coli</i> bacterial cells is the fastest and cheapest way to obtain such proteins. However, recombinant proteins, when produced in <i>E. coli</i>, often form insoluble and functionally inactive aggregates. The purpose of this work is to create a set of expression vectors for rapid screening of optimal conditions for the production of recombinant proteins prone to aggregation in <i>E. coli</i> in soluble form. The work used modern genetic engineering methods, including optimization and <i>de novo</i> synthesis of nucleotide sequences encoding helper polypeptides. For the production and chromatographic purification of proteins, the standard strain BL21(DE3) and the affinity chromatography method using a metal chelate sorbent were used. As a result, a set of nine vectors with helper polypeptides was obtained to increase solubility and increase product yield during the production of recombinant proteins in <i>E. coli</i> cells. The efficiency of obtaining protein drugs prone to molecular aggregation using this set of vectors has been demonstrated by the exampe of three cytokines: interleukin-31 (IL-31), interleukin-33 (IL-33), and transforming growth factor beta (TGF-beta1). The resulting set allows for rapid selection of a suitable helper polypeptide as well as optimization of conditions for obtaining a soluble form of recombinant proteins in <i>E. coli</i> during the pharmaceutical development of therapeutic drugs.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 3","pages":"254 - 262"},"PeriodicalIF":0.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating Non-Covalent Interactions of Human Serum Albumin with Doxorubicin and Folic Acid","authors":"I. A. Bauer,&nbsp;E. V. Dmitrienko","doi":"10.1134/S1990750823600413","DOIUrl":"10.1134/S1990750823600413","url":null,"abstract":"<p>The development of effective delivery systems for antitumor drugs with increased specificity and controlled release is one of the challenges for biomedical chemistry. This paper presents studies of non-covalent interactions of human serum albumin, which may be a promising carrier for drug delivery, with antitumor antibiotic doxorubicin and folic acid, which have the potential of a guide ligand. Intermolecular interactions of doxorubicin and folic acid with human serum albumin were studied by spectroscopic methods at various pH levels and temperatures. The binding strength of doxorubicin and folic acid responded differently to pH changes. The affinity of the drug to protein increased with the transition from acidic to alkaline conditions, while pH 7.4 was optimal for binding for folic acid. In the case of the triple system, it was found that there was no significant effect of albumin complexation with folic acid on non-covalent interaction with doxorubicin. As expected, binding to these active compounds altered the conformation of the protein. At the same time, this change was minimal in physiological pH for folic acid and in alkaline for doxorubicin. Additionally, the therapeutic properties of doxorubicin, non-covalently bound to human serum albumin, were shown to be preserved in vitro.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 3","pages":"231 - 242"},"PeriodicalIF":0.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calorie Restriction Decreases JAK-STAT Pathway Gene Expression, Tumor Mass and Activity of Glutathione-Dependent Enzymes","authors":"E. Yu. Sergeeva,&nbsp;Y. A. Fefelova,&nbsp;N. M. Titova","doi":"10.1134/S1990750824600298","DOIUrl":"10.1134/S1990750824600298","url":null,"abstract":"<p>Melanoma is one of the most aggressive types of cancer. Melanoma morbidity is increasing every year and mortality rate is high besides target therapy. Calorie restriction is lifestyle approach that can alter the activity of the pathways regulating key processes of melanomagenesis. The signaling cascades modulated by fasting includes JAK/STAT. Female С57Bl/6 mice were used for the investigation of the calorie restriction effect on the tumor growth and development. Control group is the mice on ad libitum diet, CR group is the mice on 30% calorie restricted diet. B16 melanoma cell transplantation has been carried out in the mice of the both groups after 3 months of ad libitum and CR regimes. During 15 days after melanoma cell implantation the formation of solid tumor has been occurred. Then the animals were euthanized and average mouse weight and tumor mass in both groups were evaluated. JAK/STAT gene expression was assessed by means of real-time PCR. The activity of glutathione peroxidase and glutathione-<i>S</i>-transferase and glutathione level were registered spectrophotometrically. 30% calorie restriction in mice with B16 melanoma decreased of tumor mass by 1.8 times (<i>р</i> = 0.049) as compared with the tumor mass of control group animals. <i>STAT1</i> expression increased by 1.9 times, <i>STAT3</i>, <i>STAT5b</i>, <i>STAT6</i>, <i>JAK1</i> and <i>JAK2</i> expression decreased by 2.8, 3.8, 2.6, 3 and 4.5 times accordingly. Activity of glutathione peroxidase, glutathione-<i>S</i>-transferase and glutathione level decreased by 1.6, 3 and 1.5 times accordingly compare with control group. The received data can become pathogenical basis for the creation of new adjuvant method of anticancer therapy.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 2","pages":"118 - 123"},"PeriodicalIF":0.6,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142219374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LINC00654–SOX5 mRNA-miRNA-133a Compose New RNA Panel for Colorectal Cancer (CRC): A Potential Diagnostic Panel for CRC","authors":"Shady Montaser Mohamed,&nbsp;Hadeel Medhat,&nbsp;Sarah Keshk,&nbsp;Marwa Matboli,&nbsp;Mohamed Kamel Hassan","doi":"10.1134/S199075082460016X","DOIUrl":"10.1134/S199075082460016X","url":null,"abstract":"<p>Many disorders can be accurately diagnosed using ribonucleic acids (RNAs). A panel of RNAs specific to colorectal cancer (CRC), generated in silico, was used in this study. This panel is composed of Nucleosome Assembly LINC00654 (Long Intergenic Non-Protein Coding RNA 654) long nucleolar RNA, SRY-box transcription factor 5 (sox5 mRNA) mRNA, small nucleolar RNA host gene (Sox5), and homo sapiens microRNA-133a (miR-133a) from the genetic and epigenetic database based on in silico data analysis. Validation and characterization of the proposed RNA network were done by qPCR in sera samples from 130 cases. These cases included 70 CRC patients with a malignant tumour, 40 patients with a benign tumour, and 20 healthy controls. Moreover, the panel expression was verified in a representative CRC, HT29, cell line. Our data revealed that the expression of LINC00654 and Sox5 RNAs was higher in the sera from CRC compared with the control group, while miR-133a showed the opposite expression pattern. These data may, at least in part, validate the in-silico relationship and enhance the possibility that miR-133a might be sponged by LINC00654 and thus leave the chance for Sox5 upregulation in CRC patients. Taken together, our findings may introduce a novel molecular network. Therefore, this RNA panel could be recommended as a potential diagnostic marker for CRC patients.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 2","pages":"151 - 166"},"PeriodicalIF":0.6,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142219378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructure of Mitochondria in Skeletal Muscle Weakened by Sarcopenia during Aging","authors":"V. B. Weiss,&nbsp;I. M. Vangeli,&nbsp;Ch. M. Eldarov,&nbsp;L. E. Bakeeva","doi":"10.1134/S1990750823600553","DOIUrl":"10.1134/S1990750823600553","url":null,"abstract":"<p>The processes of progressive decrease in muscle mass and weakening of mitochondrial function that occur during aging in skeletal muscles remain poorly understood as does, above all, the cause-and-effect relationship between the ultrastructure of mitochondria and atrophic processes in skeletal muscles. An ultrastructural study of the features of the internal structural organization of mitochondria during aging of skeletal muscle was carried out on representatives of rapidly aging mammalian species (Wistar rats, OXYS, mice) and representatives of long-lived species: the naked mole rat (<i>Heterocephalus glaber</i>) and human. Previously unknown, age-related structural changes in the internal organization of skeletal muscle mitochondria in OXYS rats at the age of 24 months are shown: the appearance in each mitochondria of local areas of altered arrangement of cristae in the form of stellate structures, as well as the presence of extremely large structural formations, apparently the result of destructive processes in mitochondria, as well as the appearance of mitochondria that are abnormal in size and internal ultrastructure. It was shown that structural changes in mice at the age of 10 months and naked mole rats at the age of 11 years were multidirectional. If disturbances in the normal ultrastructure in mice affected not only mitochondria but also muscle fibers and the sarcoplasmic reticulum, then not only no pathological changes are observed in mole rats but also, on the contrary, the powerfully developed structure of mitochondria indicates the functional activity of these organelles. For the first time, the ultrastructure of mitochondria in human skeletal muscle at the age of 68–81 and 25–28 years was compared using biopsy material. In elderly patients, the phenomenon of mitochondrial proliferation is shown: a compensatory structural response to mitochondrial dysfunction. Mitochondria are small, with a small number of cristae. In young people, the ultrastructure of mitochondria corresponded to classical ideas about the features of the structural organization of skeletal muscle mitochondria. Literary ideas about the possible role of autophagy in the development of aging processes are considered.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 2","pages":"132 - 143"},"PeriodicalIF":0.6,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142219376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Autophagy Regulators on FcεRI-Dependent Activation of the RBL-2H3 Cells","authors":"A. N. Pavlyuchenkova,&nbsp;M. S. Smirnov,&nbsp;M. A. Chelombitko","doi":"10.1134/S1990750824600067","DOIUrl":"10.1134/S1990750824600067","url":null,"abstract":"<p>Mast cells (MCs) are an important population of connective tissue cells that play a key role in the development of allergic diseases. The main pathway of MC activation in allergies is associated with the interaction of antigen complexes with immunoglobulin E and their subsequent binding to the FcεRI receptor. This leads to rapid release of secretory granules and cytokine production. In recent years, there has been increasing evidence for the involvement of autophagy in many processes, including MC function. Therefore, autophagy regulators can be used as potential inhibitors of MC activity and as therapeutic agents for the treatment of allergic diseases. In the present study, we investigated the effects of two autophagy inhibitors SBI-0206965 and LY294002, and one autophagy activator rapamycin on FcεRI-dependent activation of RBL-2H3, which usually used as model of MCs and basophils. Cell activation was assessed by determining the content of the secretory granule marker β-hexosaminidase and the levels of the cytokines TNF, IL-4 and IL-13. Treatment of cells with SBI-0206965 and LY294002 was shown to reduce FcεRI-dependent degranulation and IL-4 cytokine secretion by RBL-2H3 cells, while the use of rapamycin resulted in reduction of the level of IL-13 cytokine. This indicates the prospect of potential application of these autophagy regulators in the therapy of allergic diseases.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 2","pages":"167 - 173"},"PeriodicalIF":0.6,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142219379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}