Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry最新文献

{"title":"Search for New Active Substances for the Development of Pharmaceuticals Using Supercritical Fluid Extraction","authors":"A. I. Artemiev,&nbsp;K. M. Demkin,&nbsp;I. V. Kazeev,&nbsp;N. V. Menshutina","doi":"10.1134/S1990750824600511","DOIUrl":"10.1134/S1990750824600511","url":null,"abstract":"<p>The use of supercritical fluids for the extraction of biologically active substances is an extremely urgent task. Extraction in a supercritical fluid environment at temperatures up to 323 K and pressures of 16–20 MPa allows preserving the value of the extracted substances. This article discusses equipment and experimental studies of the process of supercritical fluid extraction of biologically active substances from various plant materials: aralia, ginseng, <i>Tribulus terrestris</i>, and a collection of multiphytoadaptogen. A comparative analysis of the efficiency of supercritical and traditional liquid extraction in a Soxhlet apparatus was carried out. High performance liquid chromatography was used to determine the content of active substances in the obtained extracts. It has been established that the yield of active substances during supercritical fluid extraction is higher than during traditional extraction methods. In addition, the use of ultrasound during supercritical extraction allows increasing the yield of aralosides from aralia.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"323 - 330"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saliva Glucose Determination by Biosensor: Method Comparison to Chemiluminescence Immunoassay","authors":"Rabia Şemsi,&nbsp;Umut Kökbaş,&nbsp;Burak Arslan,&nbsp;Erdal Ergünol,&nbsp;Aylin Sepici Dinçel,&nbsp;Levent Kayrın","doi":"10.1134/S1990750824600286","DOIUrl":"10.1134/S1990750824600286","url":null,"abstract":"<p>The noninvasive sampling materials and methods have garnered great interest among researchers. Saliva is considered the most popular material, and glucose is one of the most commonly used biochemical parameters. This article provides a comparison between the biosensor and autoanalyzer methods for saliva glucose measurement and identifies its potential as a point-of-care testing tool. The experiments were performed using salivary samples from twenty-six adults collected using saliva collection tubes. The swabs were soaked in saliva for 1 min and then centrifuged for 10 min at 1500 <i>g</i>. Glucose concentrations were determined using two methods: one based on an autoanalyzer using chemiluminescence assay and the other based on a biosensor using glucose oxidase enzyme-based amperometric method. A statistically significant correlation was found between the two methods (<i>r</i> = 0.802; <i>p</i> &lt; 0.001). The most remarkable result from the data is that there were no significant differences in saliva glucose measurements between the two methods. This paper presents an innovative biosensor method that can be readily used in practice for rapid results, low cost, and specificity compared to the frequently used autoanalyzer method.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"361 - 367"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Elevated Concentrations of Secretory IgA on the Level of Immunocompetent Cells, Cytokines, Reagins, Circulating Immune Complexes, and C-Reactive Protein in the Blood of Practically Healthy Residents of Murmansk Oblast","authors":"L. V. Gubkina,&nbsp;A. V. Samodova","doi":"10.1134/S1990750824600389","DOIUrl":"10.1134/S1990750824600389","url":null,"abstract":"<p>A total of 104 (77 women and 27 men) practically healthy residents of the city of Apatity, Murmansk oblast, aged 21–55 years were examined. The following indicators were studied in venous blood: the ratio and count of leukocytes, eosinophils, neutrophils, lymphocytes, and monocytes; the lymphocyte count with the phenotypes CD3+, CD4+, CD8+, CD71+, and CD95+ determined by the indirect immunoperoxidase reaction using monoclonal antibodies; the content of cytokines IL-6, IL-10, and TNF-α and immunoglobulin E; the level of secretory immunoglobulin IgA; and the content of C-reactive protein determined by the enzyme immunoassay. It was found that 31.73% of permanent residents of the city of Apatity, Murmansk oblast, had elevated levels of sIgA in their blood. People with increased sIgA concentrations in venous blood had higher counts of leukocytes, predominantly segmented neutrophilic granulocytes, increased concentrations of proinflammatory cytokines (IL-6, TNF-α) and anti-inflammatory (IL-10), reagins, small circulating immune complexes (IgG), and acute phase protein (CRP).</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"340 - 344"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peculiarities of General and Mucosal Immunity in Urban Residents of Murmansk Oblast","authors":"A. V. Samodova,&nbsp;L. V. Gubkina,&nbsp;K. O. Pashinskaya,&nbsp;S. N. Balashova,&nbsp;L. K. Dobrodeeva,&nbsp;A. A. Martynova,&nbsp;E. I. Grigor’eva","doi":"10.1134/S1990750824600407","DOIUrl":"10.1134/S1990750824600407","url":null,"abstract":"<p>The state of general and mucosal immunity was studied in 103 permanently living and working residents of Apatity town, Murmansk oblast. In examined individuals, a high level of deficiency of active neutrophilic phagocytes in the peripheral blood and on mucous membranes of the pharynx, urinary system, and intestine is established. A high frequency of registration of a deficiency of the sorption capacity of epitheliocytes and levels of secretory immunoglobulins A are detected. An increase in the concentration of pathogenic microorganisms on mucous membranes of the pharynx and urinary system and a deficiency of normal microflora on mucous membranes of the intestine are observed. Long life in unfavorable climate conditions is characterized by a high level of deficiency of mature T lymphocytes, T helpers, and T lymphocytes with a transferrin receptor and cells to programmed cell death.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"383 - 389"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143107914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the Expression Landscape of HER2-Negative Breast Cancer under the Influence of Anthracycline-Containing Neoadjuvant Chemotherapy Regimens","authors":"M. K. Ibragimova,&nbsp;M. M. Tsyganov,&nbsp;E. A. Kravtsova,&nbsp;N. V. Litviakov","doi":"10.1134/S1990750824600559","DOIUrl":"10.1134/S1990750824600559","url":null,"abstract":"<p>Currently, several treatment options are available for patients with HER2 breast cancer; however, there is no gold standard first-line treatment. Anthracycline-containing regimens are also considered traditional systemic treatment as preoperative chemotherapy for this category of patients, and they are a controversial decision due to the high degree of toxicity and low complete pathological response rates. That is why new studies are needed to identify biomarkers that predict response to anthracyclines in NAC HER2-BC, both the presence of pCR and a favorable long-term outcome, to personalize preoperative treatment. In this work, changes were studied in the expression profile of breast tumor of the luminal B HER2-negative subtype under the influence of anthracycline-containing NAC regimens in order to identify the groups of potential expression markers: an objective response to the treatment used and predicting the occurrence of hematogenous metastasis.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"368 - 382"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143107915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Synthetic Kisspeptin Derivatives Impair Wound Healing in Breast Adenocarcinoma","authors":"P. M. Kopeikin,&nbsp;E. G. Bogomolova,&nbsp;A. O. Drobintseva,&nbsp;T. S. Kleimenova","doi":"10.1134/S1990750824600560","DOIUrl":"10.1134/S1990750824600560","url":null,"abstract":"<p>Currently, cancer plays an important role in public health. Mortality from cancer in 90% of cases is caused by the development of metastases, which are the clinical result of the invasiveness of the pathology. Among the known metastasis suppressor molecules are the products of the <i>KISS1</i> gene—the metastin protein and its derivatives: kisspeptin-14 (KP14), kisspeptin-13 (KP13) and kisspeptin-10 (KP10). The KISS1/KISS1R system regulates the development and progression of several types of cancer. These molecules are important in the development of new treatment methods and/or as markers in the treatment of cancer, so the biologically active kisspeptin analogues design and synthesis is a promising direction for the development of drugs that prevent the metastases formation. In this study, promising sequences of kisspeptin and its modified analogues—KP-10, KP-10-dAla, KP-6-dAla—were designed. Peptides with a purity of &gt;98% were obtained through chemical synthesis and chromatographic purification. Study of its biological activity on the MCF-7 cell line (breast adenocarcinoma) demonstrated the anti-migration activity of the peptides. The greatest effect was observed with the treatment of 1000 nM KP-10, 10 nM KP-6-dAla, 10 nM KP-10-dAla. It was shown that replacing glycine with D-alanine and shortening the kisspeptin chain led to a slower rate of wound healing of MCF-7 cells compared to control. The potential effectiveness of KP-6-dAla and KP-10-dAla in suppressing metastasis was revealed, thereby making it a promising candidate for the development of anticancer drugs.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"345 - 354"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving the Functional and Antibacterial Properties of an Endoprosthetic Mesh Using Atomic Layer Deposition Technology","authors":"R. M. Ragimov,&nbsp;N. M. Abdullaeva,&nbsp;V. Yu. Khanaliev,&nbsp;M. A. Khamidov,&nbsp;I. M. Abdulagatov","doi":"10.1134/S199075082470001X","DOIUrl":"10.1134/S199075082470001X","url":null,"abstract":"<p>Over the past few decades, abdominal wall hernias of various locations have attracted close attention from surgeons in various countries, including Russians. Approximately 20 million hernioplasty operations are performed annually, at least 85% of which involve the use of surgical meshes. This interest of specialists in hernias is due to serious reasons, first of all, strangulation, which is a life-threatening complication and often leads to death. According to various authors, from 3 to 8% of the population suffer from hernias, and at least 20 million people in the world undergo hernia repair every day, and they are recently increasingly using endoprosthetic meshes from various manufacturers for hernial orifice repair. Often, hernia mesh causes postoperative complications, such as adhesions, wrinkling of the mesh, granulomas, fistulas, seromas, including infection of the implant, leading to the development of abscesses and possibly sepsis. To reduce these complications, various modifications of endoprosthetic meshes and materials are being developed. However, there is not a single mesh at present that would completely satisfy hernia surgeons. Based on the above, the authors set a goal to improve the functional and antibacterial properties of polypropylene endoprosthetic mesh using atomic layer deposition (ALD) nanotechnology. The ALD method consists in that a nanofilm is created on the surface of the endoprosthetic mesh through cyclic reactions using trimethylaluminum, titanium tetrachloride, and vanadium trichloroxide. The authors have obtained the first samples of meshes that were implanted into the bodies of experimental animals. The experimental samples demonstrated good biocompatibility and integration into the surrounding tissue, preventing any inflammatory response. The developed method for improving the functional and antibacterial properties of endoprosthetic meshes is simple in industrial implementation compared to PACVD or CVD and allows covering large areas, therefore, reducing material production costs and increasing the economic efficiency of domestically produced mesh implants and making them competitive in the world market.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"355 - 360"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Arachidonic Acid Metabolizing Cytochromes P450 in the Control of Cardiovascular Functions","authors":"M. L. Perepechaeva,&nbsp;A. Yu. Grishanova","doi":"10.1134/S1990750824600158","DOIUrl":"10.1134/S1990750824600158","url":null,"abstract":"<p>Arachidonic acid (AA) is widely distributed in many organs and can be metabolized in several ways into small lipid molecules: eicosanoides that perform a physiological and/or pathophysiological role. Among them, epoxyeicosatrienoic acids (EET) and hydroxyeicosatetraenoic acids (HETE), which are produced by cytochrome P450 (CYP) enzymes, have attracted much attention. AA metabolites play an important role in such cellular processes as proliferation, differentiation, and apoptosis and are also involved in the development of organs and tissues as well as in the pathogenetic processes underlying the development of various diseases; in particular, AA metabolites formed with the participation of CYP are involved in normal and pathological processes associated with the functioning of the cardiovascular system<i>.</i> This review focuses on the formation and cardiovascular effects of CYP-generated AA metabolites and summarizes current literature data on the physiological and pathophysiological significance of the CYP-dependent AA metabolic pathway and its relevance to the cardiovascular system in both health and disease.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 3","pages":"192 - 213"},"PeriodicalIF":0.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Elevated Concentrations of Antibodies on the Immune Response in Patients with Autoimmune Thyroiditis","authors":"K. V. Vereshchagina,&nbsp;A. V. Samodova,&nbsp;L. K. Dobrodeeva","doi":"10.1134/S1990750823600565","DOIUrl":"10.1134/S1990750823600565","url":null,"abstract":"<p>A study is performed of the effect elevated concentrations of antibodies have on the immune response in patients with autoimmune thyroiditis (AIT) living in Arkhangelsk. Results from immunological examinations of 108 individuals with AIT are presented. The immunological study included examining hemograms, the contents of phenotypes of lymphocytes, cytokines, immunoglobulins, and antibodies of thyroid peroxidase (TPO), double-stranded DNA, and nucleoproteins. It was estanlished that a rise in the concentration of antibodies (AB) to TPO, ds-DNA, to RNP in patients with AIT is associated with elevated contents of mature T lymphocytes (CD3+), cytotoxic T lymphocytes (CD8+), cells labeled for programmed cell death (CD95+), and a drop in concentrations of IL-10. An increase in the concentration of cytotoxic lymphocytes and cells labeled for apoptosis and a simultaneous drop in the concentration of regulatory IL-10 is not beneficial in terms of prognostic value for the accumulation of autoantigens and autoantibodies.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 3","pages":"243 - 248"},"PeriodicalIF":0.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of miR-21, miR-378a, miR-205, and Their Targets in ER-Positive Breast Tumors with Different HER2 Protein Levels","authors":"G. R. Abdullin,&nbsp;T. S. Kalinina,&nbsp;V. V. Kononchuk,&nbsp;D. A. Obukhova,&nbsp;I. S. Valembakhov,&nbsp;D. D. Zakharova,&nbsp;S. I. Makarova,&nbsp;L. F. Gulyaeva","doi":"10.1134/S1990750824600195","DOIUrl":"10.1134/S1990750824600195","url":null,"abstract":"<p>Tyrosine protein kinase HER2 plays an important role in carcinogenesis. In breast cancer (BC), the <i>HER2</i> gene is amplified in approximately 20% of cases. Trastuzumab is used to treat BC with <i>HER2</i> gene amplification. Trastuzumab is not effective in treating tumors with low HER2 expression, but trastuzumab deruxtecan was recently found to significantly improve prognosis in these patients. Nonetheless, there are still difficulties with accurate diagnosis of HER2-low BC, especially at the preoperative stage. In this study, when comparing the results between core needle biopsies and resection specimens using three immunohistochemistry scores (0, 1+, and 3+) of the HER2 protein level, we observed only moderate agreement (66.2%, κ = 0.486) in patients who did not undergo neoadjuvant therapy (<i>n</i> = 71). Other miRNA- or protein-coding genes regulated by HER2 signaling pathways may be additional markers for the scoring of the HER2 level. We measured levels of HER2-regulated miR-378a, -205, and -21 and mRNA levels of their target genes <i>TRPS1</i>, <i>ITGA2</i>, <i>BCL6</i>, and <i>PTEN</i> in BC surgical specimens. For estrogen receptor-positive BC (<i>n</i> = 64), we confirmed that the expression of miR-21, miR-378a, <i>TRPS1</i>, <i>PTEN</i>, and <i>BCL6</i> is associated with the HER2 protein level. Moreover, the expression profile of miR-378a, <i>BCL6</i>, and <i>PTEN</i> differed between HER2 1+ and HER2 3+ tumors. <i>TRPS1</i> expression was significantly higher in HER2 3+ tumors compared with HER2 0 tumors, but there was no difference in the amount of <i>TRPS1</i> mRNA between HER2 1+ and HER2 3+ tumors. Thus, an analysis of the expression of miR-21, miR-378a, <i>TRPS1</i>, <i>PTEN</i>, and <i>BCL6</i> may help to distinguish between HER2-negative, HER2-positive, and HER2-low BCs.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 3","pages":"275 - 285"},"PeriodicalIF":0.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}