Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry最新文献

{"title":"The Influence of Vitamins D and K on Some Biochemical, Cytochemical, and Clinical Blood Parameters In Vivo with Separate and Combined Administration","authors":"O. V. Popova,&nbsp;A. V. Mikhailov,&nbsp;G. P. Drobot,&nbsp;N. V. Turmukhametova,&nbsp;V. P. Manaeva,&nbsp;T. V. Ivanova,&nbsp;Y. A. Kupsoltseva,&nbsp;S. B. Mustafaeva,&nbsp;R. I. Bulgariev","doi":"10.1134/S1990750824601176","DOIUrl":"10.1134/S1990750824601176","url":null,"abstract":"<p>A comprehensive study was conducted on the effect of vitamins D and K on some indicators of lipid peroxidation and the overall antioxidant activity of blood plasma in rats of two age groups. Differences in the studied parameters were revealed both in terms of age and under the influence of administered vitamins. An antioxidant effect of vitamin D is noted in adult animals, which is expressed in a significant decrease in the level of malondialdehyde (MDA) in blood plasma. In young people, a similar effect was found with the combined action of vitamins D and K. The content of lipids, glycogen, and cationic proteins in blood neutrophils of animals receiving vitamins D and K separately and together was analyzed. Significant changes in the content of cationic proteins were revealed when vitamin K was administered to adult animals. When analyzing the leukocyte formula, vitamin K and a complex of vitamins D and K showed the greatest effect, reflected in a change in the proportion of monocytes and lymphocytes. A negative correlation was shown between the lipid content in blood neutrophils and the level of one of the lipid peroxidation products, MDA, in blood plasma.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S82 - S90"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Kisspeptin Preparations on the Expression of CRH, GRH, GNRHR1, and OXT Genes in the Brain of Danio rerio after Social Isolation","authors":"A. P. Perova,&nbsp;V. A. Golts,&nbsp;A. V. Lizunov,&nbsp;E. A. Sekste,&nbsp;E. R. Bychkov,&nbsp;A. A. Bairamov,&nbsp;A. A. Lebedev,&nbsp;P. D. Shabanov","doi":"10.1134/S199075082460122X","DOIUrl":"10.1134/S199075082460122X","url":null,"abstract":"<p>The effect of kisspeptin preparation on the expression of the genes of hormonal regulation of sexual behavior in <i>Danio rerio</i> fish was studied after the effect of social isolation. For this, fish were placed in individual containers for 48 h to model the conditions of social isolation, after which kisspeptin was administered, and they were again placed in the conditions of social isolation for 1 day. It was established that changes in the expression of the <i>crh</i>, <i>grh</i>, <i>gnrhr1</i>, and <i>oxt</i> genes occurred in the fish brain after social isolation upon administration of kisspeptin peptide. It is interesting that the expression of these genes changed both under isolation conditions and during subsequent effect of kisspeptin, which indicates possible mechanisms of regulating stress and behavioral responses through hormonal systems.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S69 - S74"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Quantitative Determination of the Total Antioxidant Activity of Tears","authors":"V. V. Tiulina,&nbsp;I. I. Senin","doi":"10.1134/S1990750824600961","DOIUrl":"10.1134/S1990750824600961","url":null,"abstract":"<p>In the present study, the authors developed a protocol for determining the total antioxidant activity of tears. For this purpose, a chemiluminescent model of free-radical oxidation of luminol initiated in the presence of hemoglobin and hydrogen peroxide was used. This approach allows for controlling the kinetics of inhibition of luminol oxidation by tears. Total antioxidant activity was evaluated by the induction period, which showed the delay time of the initiation of luminol oxidation. The calibration curve for tear fluid was linear within the range from 0.5 to 7 μL (<i>r</i>² = 0.972). The advantages of the developed protocol are high sensitivity, reproducibility, and rapidity of the assay. This makes our protocol suitable for determining the total antioxidant activity of tears.\u0000\u0000<b>Keywords</b>: total antioxidant activity, tear fluid, antioxidants, chemiluminescence</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S18 - S25"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Genetic Determinants of Pregnancy Pathology","authors":"A. B. Kazumova","doi":"10.1134/S1990750824600882","DOIUrl":"10.1134/S1990750824600882","url":null,"abstract":"<p>In the modern world, scientists often discuss problems associated with complications of pregnancy and childbirth in women. The study of cases of repeated fetal loss, stillbirth, intrauterine growth retardation and preeclampsia leads to the discovery of new aspects of this pathology. Of particular interest is Upshaw−Schulman syndrome, a rare congenital form of thrombotic thrombocytopenic purpura caused by mutations in the ADAMTS13 gene. This gene encodes a metalloproteinase capable of cleaving von Willebrand factor, which is important for blood clotting processes. A review of modern Russian and foreign, primarily English-language, literature was conducted on methods of diagnosis and treatment of congenital thrombocytopenic purpura as well as the prevention of complications of pregnancy and childbirth. This review highlights the main mechanisms of development and progression of the syndrome, current directions of management of women with Upshaw−Schulman syndrome, and methods of therapy of associated reproductive failure, and also presents modern groups of pharmacological drugs of choice. Pregnancy with Upshaw−Schulman syndrome is becoming a major focus of research in obstetrics, and it is important to conduct further high-quality association studies to develop innovative therapeutic options and options in the future.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S44 - S50"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Mechanisms of Antifibrotic Action of Soluble Proteins of Human Mesenchymal Stromal Cell Secretome","authors":"M. Kulebyakina,&nbsp;D. Butuzova,&nbsp;O. Klychnikov,&nbsp;Yu. Strogov,&nbsp;N. Basalova,&nbsp;A. Efimenko","doi":"10.1134/S1990750824601085","DOIUrl":"10.1134/S1990750824601085","url":null,"abstract":"<p>Multipotent mesenchymal stromal cells (MSCs) are key regulators of tissue healing after injury. Acting in a paracrine manner, MSCs promote tissue regeneration and prevent the development of fibrosis, a pathological condition in which there is excessive replacement of functional tissue with scar tissue. However, the mechanisms of the antifibrotic action of proteins secreted by MSCs have not yet been established. The authors previously showed that the fraction of soluble protein factors (SF) of the MSC secretome is capable of suppressing the key cellular mechanism of fibrosis development, the differentiation of fibroblasts into myofibroblasts, and also established, using proteomic analysis, that this fraction is enriched in proteins capable of regulating the canonical signaling pathways of transforming growth factor beta, Wnt, and Notch in fibroblasts. This study investigated the effect of the SF fraction on these signaling pathways in human skin fibroblasts and found that this fraction suppresses the canonical Wnt signaling pathway in a model of myofibroblast differentiation of fibroblasts. According to the results of proteomic analysis, SF is enriched in the DKK3 protein, which is able to regulate fibroblast differentiation through interaction with components of the canonical Wnt signaling pathway. It was shown that depletion of DKK3 protein in the SF fraction of MSC secretome by immunoprecipitation results in a significant reduction in the ability of the SF fraction to suppress fibroblast differentiation into myofibroblasts in vitro. The obtained data clarify the mechanism of the antifibrotic action of MSC secretome proteins associated with the suppression of differentiation of fibroblasts into myofibroblasts.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S51 - S60"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Features of the Genetically-Encoded Biosensor to PIP3 Influence on Insulin Signaling in MSCs","authors":"A. P. Fedorovsky,&nbsp;M. N. Karagyaur,&nbsp;N. S. Voloshin,&nbsp;M. A. Vigovskiy,&nbsp;V. I. Chechekhin,&nbsp;A. A. Zinoveva,&nbsp;E. Bakhchinyan,&nbsp;E. S. Chechekhina,&nbsp;P. A. Tyurin-Kuzmin,&nbsp;L. K. Muranova,&nbsp;K. Y. Kulebyakin","doi":"10.1134/S1990750824601103","DOIUrl":"10.1134/S1990750824601103","url":null,"abstract":"<p>Genetically encoded fluorescent biosensors (GEBs) are extensively utilized for investigating intracellular signaling processes. Nevertheless, the potential impact of these sensors on intracellular signaling pathways and overall cellular functionality is frequently overlooked, which may lead to inaccuracies in experimental results. In this study, the authors examined the effects of a widely used fluorescent biosensor, which binds to phosphatidylinositol 3,4,5-trisphosphate (PIP3), on the phosphoinositide 3-kinase (PI3K)-signaling pathway in an immortalized multipotent mesenchymal stromal cell (MSC) line. To achieve this, two immortalized MSC lines with distinct levels of biosensor expression were generated. The introduction of the biosensor led to a reduction in the adipogenic potential of these cell lines compared to the parental line. This effect was attributed to the inhibition of the PI3K signaling pathway, which is activated by insulin—a critical regulator of adipogenic differentiation. The biosensor to PIP3 interfered with the recruitment of downstream effectors, namely PDK and Akt. This was evidenced by diminished levels of phosphorylated Akt (p-Akt) following insulin stimulation in the transduced cell lines relative to the parental line, with the cell line exhibiting higher biosensor expression showing lower p-Akt levels than the line with lower expression. In contrast, the levels of phosphorylated ERK, a marker of another insulin-dependent signaling pathway, remained consistent across all cell lines, indicating that the biosensor selectively inhibited the PIP3-dependent pathway. This case emphasizes the necessity of a careful and cautious approach when employing GEBs in biological research. Prior to their use, it is imperative to verify that these biosensors do not disrupt intracellular signaling cascades or alter cellular behavior.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S91 - S98"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Organotin Complexes with Losartan: Synthesis and Biological Activity","authors":"T. A. Antonenko,&nbsp;O. A. Raykova,&nbsp;Yu. A. Gracheva,&nbsp;D. M. Mazur,&nbsp;V. A. Roznyatovsky,&nbsp;R. I. oglu Musaev,&nbsp;N. A. Gurova,&nbsp;M. S. Pshenichnikova,&nbsp;D. S. Yakovlev,&nbsp;E. R. Milaeva,&nbsp;A. A. Spasov","doi":"10.1134/S1990750824601334","DOIUrl":"10.1134/S1990750824601334","url":null,"abstract":"<p>Novel organotin(IV) complexes based on losartan have been synthesized and characterized by ¹H, ¹³C, ¹¹⁹Sn NMR, IR, ESI-MS and elemental analysis. The antiproliferative activity of the complexes was assessed in vitro against human cancer cells, while their cytotoxic effects were examined on primary rat skin fibroblasts. The compounds exhibited strong activity, significantly inducing apoptosis. Additionally, they effectively suppressed the spasmogenic effect induced by angiotensin II in isolated rat portal veins.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S6 - S17"},"PeriodicalIF":0.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenetic Potential of the Epigenetic Status of Genes of Mendelian Forms of Thoracic Aortic Aneurysms","authors":"A. N. Kucher,&nbsp;Iu. A. Koroleva,&nbsp;M. S. Nazarenko","doi":"10.1134/S1990750824600183","DOIUrl":"10.1134/S1990750824600183","url":null,"abstract":"<p>Rare pathogenic variants in genes whose protein products form the structure of the extracellular matrix, regulate smooth muscle cell function, or belong to the TGFβ/SMAD signaling pathway are detected in 20–30% of thoracic aortic aneurysm (TAA) cases. In addition to the structural changes, epigenetic events associated with these genes may influence the risk of aortic aneurysm. This review summarizes the published study results on the functional significance of epigenetic modifications in the genes of Mendelian forms of TAA. Most TAA genes are characterized by overlapping localization with genomic noncoding regulatory elements (including genes of microRNAs, long noncoding RNAs, etc.). These genomic regions have not been practically studied in patients with aortic aneurysm, but a number of noncoding RNAs (<i>ACTA2-AS1</i>, <i>TGFB2-AS1</i>, <i>PRKG1-AS1</i>) have been shown to be involved in the regulation of processes pathogenetically significant for aortic aneurysm (proliferation, apoptosis, autophagy, inflammation, endothelial dysfunction) and metabolic pathways (TGFβ/SMAD, Wnt/β-catenin). In the regions of TAA genes, the changes in DNA methylation are also recorded both in the presence of risk factors (structural features of the aorta, blood flow patterns) and in the development of aortic aneurysm. Various epigenetic events may also exert the pathogenic effects of aortic aneurysm-associated genetic variants localized in TAA genes. Thus, despite the fact that the long noncoding RNA and microRNA genes located in the TAA gene region and various epigenetic events (including histone modifications and DNA methylation) have not been sufficiently studied in this aortopathy, the information provided in scientific publications allows us to consider these epigenetic markers as promising regulators of certain pathogenetic processes in the development of an aortic aneurysm.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"299 - 322"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Procarcinogenic Characteristics of Tryptophan Metabolism in Obese Patients","authors":"O. P. Shatova,&nbsp;A. A. Zabolotneva,&nbsp;A. V. Shestopalov","doi":"10.1134/S1990750824600766","DOIUrl":"10.1134/S1990750824600766","url":null,"abstract":"<p>Tryptophan is an essential amino acid, the catabolism of which occurs in the human body along three main pathways: kynurenine, serotonin, and indole. At the same time, a significant part of tryptophan metabolites is formed as a result of the enzymatic activity of the intestinal microbiota. Deviations in tryptophan metabolism, manifested in the form of shifts towards the formation of certain metabolites, are characteristic of some pathological conditions, in particular obesity and malignant neoplasms. It is known that the growth of some types of tumors is associated with excess body weight, but the mechanisms of the procarcinogenic effect of obesity remain not fully understood. Obesity is characterized by the development of systemic inflammation and increased production of proinflammatory cytokines, which increase the expression of the key enzyme of the kynurenine pathway and intensify the formation of immunosuppressive and cytotoxic metabolites: kynurenine and quinolinic acid. Increased stimulation of the kynurenine pathway in the body of obese and cancer patients leads to a decrease in tryptophan itself, which is necessary for the proliferation of immunocompetent cells and a normal immune response. Both obese and cancer patients are characterized by increased levels of quinolinic acid and decreased concentrations of indole-3-propionate in the blood serum. Obese patients are often characterized by a decrease in serotonin in the blood serum, while not only an increase in serotonin production has been established for tumor tissues but also an increase in the expression of various types of serotonin receptors. The existing general trends in changes in tryptophan metabolism indicate the presence of intersection points in the pathogenesis of obesity and tumor development. A literature review was conducted using the PubMed database, primarily using literature sources from 2018–2024.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"287 - 298"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Technology for Applying Nanocoatings to Surgical Meshes and Study of the Functional Properties of the Resulting Nanofilms","authors":"N. M. Abdullaeva,&nbsp;R. M. Ragimov,&nbsp;V. Yu. Khanaliev,&nbsp;I. M. Abdulagatov,&nbsp;M.A. Khamidov","doi":"10.1134/S1990750824700021","DOIUrl":"10.1134/S1990750824700021","url":null,"abstract":"<p>—Today, abdominal hernias of various localizations are diagnosed in approximately 7% of the world’s population. Approximately 20 million hernioplasty operations are performed annually, 85% of which use surgical mesh. The implant itself often becomes the cause of the development of postoperative complications in the form of adhesions, granulomas, fistulas, seromas, abscesses, etc. The study involved surgical meshes with a nanocoating applied using atomic layer deposition (ALD) technology. The aims of the study were the development of a technology for applying a nanocoating to impart antibacterial properties to surgical endoprosthetic meshes and improve their biocompatible properties using the atomic layer deposition method. During the work, a nanocoating was applied to the surface of the polypropylene meshes using the atomic layer deposition (ALD) method to impart antibacterial properties to the implant. As a result of applying the nanofilm, nano-sized roughness was formed on the surface, which prevented the adhesion of microorganisms. At the same time, the main characteristics of the grids themselves did not change. By means of cyclic reactions, using trimethylaluminum, titanium tetrachloride, and vanadium trichloroxide, the first samples of meshes were obtained, which were introduced into the bodies of experimental animals. Complex coating (Al₂O₃ + TiO₂/V₂O₅) demonstrated good integration into the surrounding tissue with minimal inflammatory response. The developed method for obtaining nanocoatings to improve the bactericidal properties of surgical endoprosthesis mesh is simple in industrial implementation, compared to PACVD or CVD, and covers large areas simultaneously, therefore reducing material costs for production and increasing economic efficiency.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 4","pages":"331 - 339"},"PeriodicalIF":0.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}