Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry最新文献

{"title":"The Effect of Methylene Blue and Its Metabolite—Azure I—on Bioenergetic Parameters of Intact Mouse Brain Mitochondria","authors":"A. P. Gureev,&nbsp;N. A. Samoylova,&nbsp;D. V. Potanina,&nbsp;V. N. Popov","doi":"10.1134/S1990750822020044","DOIUrl":"10.1134/S1990750822020044","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>Methylene blue, a phenothiazine dye, that is widely used in medicine and is under clinical trials as an agent for treatment of Alzheimer’s disease. One of the factors of the unique therapeutic effect of methylene blue is its redox properties, allowing implementation of alternative electron transport: the dye accepts electrons from reducing equivalents in mitochondria and transfer them to other components of the respiratory chain or molecular oxygen. Azure I, an <i>N</i>-dimethylated metabolite of methylene blue, is potentially a more effective compound than methylene blue, but its ability for alternative electron transport has not been studied yet. We have shown that in contrast to methylene blue, azure I is unable to restore the membrane potential in isolated mouse brain mitochondria, inhibited by rotenone and, therefore, is unable to perform bypass of the respiratory chain complex I. Moreover, addition of azure I does not affect the rate of mitochondrial respiration in contrast to methylene blue, which increases the rate of non-phosphorylation respiration. At the same time, both dyes stimulate an increase in H₂O₂ production. Thus, only methylene blue is capable of alternative electron transport, while azure I does not produce complex I bypass. This limits its therapeutic application only as a mitochondrial-targeted agent, but does not question its antidepressant effects.</p></div></div>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"16 2","pages":"148 - 153"},"PeriodicalIF":0.6,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S1990750822020044.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4695431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Bioinformatic Identification of Proteins with Varying Levels of Post-Translational Modifications in Experimental Ischemic Stroke in Mice","authors":"V. S. Skvortsov,&nbsp;Ya. O. Ivanova,&nbsp;A. I. Voronina","doi":"10.1134/S199075082202007X","DOIUrl":"10.1134/S199075082202007X","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>The experimental data obtained by Simats, A. et al. (<i>Molecular and Cellular Proteomics</i>, 2020, vol. 19, no. 12, pp. 1921–1936) were analyzed using a bioinformatic aproach. Original experimental results available in the ProteomeXchange database were obtained using a comprehensive multiomics approach to identify potential blood biomarkers in ischemic stroke in mice. The identification of peptides with post-translational modification (PTM) was performed by us using the raw data (accession code PXD016538). Only phosphorylation and deamination were considered as PTMs. Different combinations of data sets (ischemic tissue with intact tissue, ischemic tissue with control taken from mice after sham surgery, etc.) were compared both in terms of the ratio of abundance for the modified peptide to the unmodified variant and in terms of absolute values of abundance. The most probable change in the PTM levels was shown for 27 proteins, which included dynamin, glycogen phosphorylase and 70 kDa heat shock protein.</p></div></div>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"16 2","pages":"113 - 124"},"PeriodicalIF":0.6,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4698129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the Risk of Tumor Progression in Patients with Early Stages of Adenocarcinoma and Squamous Cell Lung Carcinoma Based on Laboratory Parameters","authors":"A. D. Tahanovich,&nbsp;N. N. Kauhanka,&nbsp;V. I. Prohorova,&nbsp;D. I. Murashka,&nbsp;O. V. Gotko","doi":"10.1134/S1990750822020081","DOIUrl":"10.1134/S1990750822020081","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>Non-small cell lung cancer (NSCLC) dominates in the morbidity of lung cancer. In stage I, only 60–70% patients overcome the 5-year survival barrier, and in stage II 5-year survival rate is declining to 35–40%. The reason for such a high mortality is relapse of the disease. The main histological forms of NSCLC are adenocarcinoma (AС) and squamous cell carcinoma (SCC). They differ in course, protocols and effectiveness of treatment. Comparative survival data for AC and SCC are controversial, and reliable biomarkers for determining the risk of tumor progression still have not been found. In order to determine the risk of disease progression we have investigated the possibility of using laboratory parameters characterizing the level of some blood proteins involved in carcinogenesis in patients with early stages of AС and SCC. We retrospectively analyzed the duration of relapse-free period after surgical treatment for one year in 1250 patients (816 with stages I and II AC, G1-3 and 434 with early stages of SCC, G1-3). In 81 AC patients and 36 SCC patients (stages I−II, G1-3) we determined the level of CYFRA 21-1 and SCC, TPA, chemokines CXCL5, CXCL8, pyruvate kinase M2, HIF-1α, hyaluronic acid and receptors CXCR1, CXCR2, CD44v6. Using the Kaplan−Meier graphical analysis, groups of low (stage I G1-2 + stage II G1) and high (stage I G3 + stage II G2-3) risk of tumor progression were identified. The one-year survival rate of AС patients was higher than in SCC patients. AС patients with high risk of tumor recurrence had higher levels of CYFRA 21-1, the mean intensity of fluorescence (MFI) of CXCR1 receptor in granulocytes, and the relative content of CXCR2 receptor in lymphocytes than patients with low risk. In the case of rapid disease progression in SCC patients, the relative content of CXCR2 receptor in lymphocytes, the proportion of monocytes supplied with CD44v6 receptor, and SCC level were higher than in patients with slow progression. Regression equations, including combinations of the above parameters (threshold value for AC—0.512, for SCC—0.409, sensitivity—91.9% and 90.0%, specificity—90.0% and 87.5%, respectively), allow to predict the probability of tumor recurrence.</p></div></div>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"16 2","pages":"154 - 163"},"PeriodicalIF":0.6,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4696699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and Internalization of Extracellular Vesicles in Norm and under Conditions of Hyperglycemia and Insulin Resistance","authors":"N. V. Yunusova,&nbsp;E. E. Dandarova,&nbsp;D. A. Svarovsky,&nbsp;N. S. Denisov,&nbsp;D. N. Kostromitsky,&nbsp;M. R. Patysheva,&nbsp;O. V. Cheremisina,&nbsp;L. V. Spirina","doi":"10.1134/S199075082202010X","DOIUrl":"10.1134/S199075082202010X","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>Extracellular vesicles (EVs) are spherical functionally important structures of cell membrane origin ranging in size from 40 nm to 5000 nm. They are involved in the horizontal transfer of mainly proteins and microRNAs. The mechanisms of EV internalization include clathrin-dependent endocytosis, caveolin-dependent endocytosis, raft-mediated endocytosis, and macropinocytosis. Type 2 diabetes mellitus (T2DM) is a common group of metabolic disorders in adults; the incidence and prevalence of T2DM increase in parallel with the obesity epidemic. Since adipose tissue plays a key role in the development of insulin resistance, EVs secreted by adipose tissue may be considered as an information transmitter in this process. EVs of the adipocyte origin are predominantly captured up by tissue macrophages, adipocytes themselves, hepatocytes, and skeletal muscles. The EV uptake promotes M1 polarization of macrophages, a decrease in glucose uptake by hepatocytes and myocytes due to the transfer of functionally active microRNAs influencing carbohydrate and lipid metabolism. Patients with T2DM and impaired glucose tolerance, have a significantly higher level of CD235a-positive (erythrocyte) EVs and a trend to the increase in CD68-positive (leukocyte) and CD62p-positive (platelet/endothelial cells) EVs. The levels of CD31+/CD146-positive EVs (endothelial cells) were comparable between diabetic and euglycemic patients. EVs from diabetic patients were preferentially internalized by monocytes (predominantly classical and transitional, and to a lesser extent nonclassical monocyte fractions) and B cells as compared to euglycemic patients. Internalization of EVs from patients with T2DM by monocytes led to a decrease in their apoptosis, changes in differentiation, and suppression of monocyte reactions controlling oxidative stress. Thus, insulin resistance increases the secretion of EVs, which are preferentially internalized by monocytes and alter their function. EVs are considered as sources of promising clinical markers of insulin resistance, complications of diabetes mellitus (endothelial dysfunction, retinopathy, nephropathy, neuropathy), and EV markers can also be used to monitor the effectiveness of therapy for these complications.</p></div></div>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"16 2","pages":"104 - 112"},"PeriodicalIF":0.6,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4697679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integral Tests of the Hemostasis System in Assessing the Efficiency of Acetylsalicylic Acid in Patients with Ischemic Heart Disease","authors":"O. S. Melnichnikova,&nbsp;I. A. Nazarova,&nbsp;O. V. Sirotkina,&nbsp;A. V. Panov,&nbsp;I. T. Abesadze,&nbsp;M. Z. Alugishvili,&nbsp;N. L. Lokhovinina,&nbsp;T. V. Vavilova","doi":"10.1134/S199075082201005X","DOIUrl":"10.1134/S199075082201005X","url":null,"abstract":"<p>Despite the fact that acetylsalicylic acid (ASA) is the “gold” standard for the prevention of cardiovascular complications in patients with coronary heart disease (CAD), some patients still have risks of atherothrombosis. In the present study, the antithrombotic effect of ASA in patients with CAD was assessed using integral hemostasis tests: the T-TAS system (Total Thrombus-formation Analysis System) and the thrombin generation test (TGT) in platelet-rich plasma (PRP). The study involved 34 patients with stable CAD (11 women, 23 men) and 33 people (15 women, 18 men) in the control group. Evaluation of the activity of thrombus formation by means of the T-TAS system revealed a significant decrease in the area under the curve (AUC10) in the group of CAD patients as compared with the control (135.6 [88.0–222.3] and 260.5 [217.3–301.9], <i>p</i> &lt; 0.05). The TGT analysis in PRP has shown that in CAD patients, there was a decrease in the peak concentration of thrombin (PTh) and the rate of thrombin (VI) formation (100.5 [78.3–127.7] and 125.7 [118.7–161.5], nmol; 7.2 [4.7–11.1] and 14.9 [11.6–18.1], nmol/min, <i>p</i> &lt; 0.01, respectively), and an increase in time parameters of the test. The number of patients with high platelet reactivity detected by T-TAS was higher than those detected by TGT (38.2% (<i>n</i> = 13) and 20.8% (<i>n</i> = 7), respectively). In the group of CAD patients correlation analysis revealed a relationship between an increase in VI with an increase in AUC10 according to T-TAS data (<i>r</i> = 0.37; <i>p</i> = 0.03). Thus, the study, T-TAS and TGT in PRP have shown their effectiveness in assessing the antithrombotic properties of ASA in CAD patients.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"16 1","pages":"60 - 65"},"PeriodicalIF":0.6,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4863059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increase in the Level of Orexin Receptor 1 (OX1R) mRNA in the Brain Structures of Rats Prone to Impulsivity in Behavior","authors":"E. A. Sekste,&nbsp;A. A. Lebedev,&nbsp;E. R. Bychkov,&nbsp;M. I. Airapetov,&nbsp;K. E. Gramota,&nbsp;I. Yu. Tissen,&nbsp;P. D. Shabanov","doi":"10.1134/S1990750822010085","DOIUrl":"10.1134/S1990750822010085","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>Orexin and its receptors (OXR) are involved in the mechanisms of pathological craving for alcohol and psychoactive drugs. The orexin system is also involved in the mechanisms of non-chemical forms of addiction: binge eating and gambling. The aim of this study was to investigate the level of orexin receptor mRNA in the hypothalamus, hippocampus, and prefrontal cortex of rats prone to impulsivity in behavior in a model for studying the elements of gambling addiction (a variant of the Iowa Gambling Task test). Brain structures were isolated on the 22nd day of the experiment. The <i>OX1R</i> gene expression was higher in the hypothalamus by 122% and in the hippocampus—by 149% in rats preferring to receive a high reward, but with a low probability as compared with a group of animals that preferred a low level of reinforcement, but with a 100% probability. On the contrary, no significant changes were observed in the level of <i>OX1R</i> mRNA in the prefrontal cortex. The level of <i>OX2R</i> mRNA insignificantly changed in the hypothalamus, hippocampus, and prefrontal cortex of rats prone to impulsivity in behavior. The data obtained suggest involvement of <i>OX1R</i> in the hypothalamus and hippocampus in the mechanisms mediating impulsive behavior and the choice of the significance of positive reinforcement in terms of its varying strength and probability.</p></div></div>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"16 1","pages":"38 - 44"},"PeriodicalIF":0.6,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4863073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impairments in Metabolism of Amino Acids—Precursors of Gasotransmitters—in the Premature Birth","authors":"A. A. Mikhelson,&nbsp;T. N. Pogorelova,&nbsp;V. O. Gunko,&nbsp;A. A. Nikashina,&nbsp;N. V. Palieva","doi":"10.1134/S1990750822010061","DOIUrl":"10.1134/S1990750822010061","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>The content of amino acids, the sources of gasotransmitters and activities of enzymes involved in their metabolism have been studied in the placenta and amniotic fluid in full-term and complicated by preterm birth. Determination of amino acids was performed using an automatic analyzer and enzyme activities were assayed by means of specific spectrophotometric methods. The development of preterm birth was accompanied by notable changes in metabolism amino acids already in the second trimester of pregnancy. Differently directed correlations were found between the level of amino acids and the activity of the corresponding enzymes. The imbalance of amino acids in the fetoplacental system in preterm birth was accompanied by changes in the production of their low molecular weight derivatives—gasotransmitters (NO, CO, H₂S), playing an important role in the regulation of numerous metabolic processes.</p></div></div>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"16 1","pages":"54 - 59"},"PeriodicalIF":0.6,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4567147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Study of mRNA Expression Profiles of Main Cell Function Regulator Genes in Unchanged Colon Mucosa from Healthy Donors","authors":"M. V. Zakharenko,&nbsp;V. K. Bozhenko,&nbsp;Ya. Yu. Kiseleva,&nbsp;E. L. Dzhikiya,&nbsp;U. S. Stanoevich,&nbsp;T. M. Kulinich,&nbsp;N. V. Melnikova,&nbsp;A. L. Senchukova,&nbsp;A. B. Urakova,&nbsp;I. B. Grunin,&nbsp;S. V. Goncharov,&nbsp;O. P. Bliznyukov,&nbsp;V. A. Solodkiy","doi":"10.1134/S1990750822010115","DOIUrl":"10.1134/S1990750822010115","url":null,"abstract":"<p>A comparative analysis of molecular genetic phenotypes of mucous membrane cells has been carried out in five anatomical regions of the large intestine in a group of healthy donors. The mRNA expression profiles of 62 genes involved in the regulation of vital cellular function were investigated in 181 biopsy samples of morphologically unchanged colon mucosa, obtained from the colon (ascending, transverse, descending, sigmoid) and rectum sections during prophylactic colonoscopy of 58 donors without large intestine pathology. The mRNA levels for 62 genes involved in the regulation of apoptosis, proliferation, transcription, differentiation, cell-cell adhesion, and immune response were assessed by RT-PCR. Statistically significant differences were found for the molecular phenotypes of five sections of the large intestine. The results of the study can serve as a basis for creating a reference database (values of expression profiles), developing methods of differential diagnostics and screening of various pathologies of the large intestine.</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"16 1","pages":"22 - 29"},"PeriodicalIF":0.6,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4567149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Efficiency of Determining CXCR1, CXCR2 and Hyaluronic Acid in Blood of Patients with Non-Small Cell Lung Cancer","authors":"D. I. Murashka,&nbsp;A. D. Tahanovich,&nbsp;M. M. Kauhanka,&nbsp;V. I. Prokhorova,&nbsp;O. V. Gotko","doi":"10.1134/S1990750822010073","DOIUrl":"10.1134/S1990750822010073","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>Non-small cell lung cancer (NSCLC) accounts for most cases of lung cancer. Histologically, it is subdivided into two histological subtypes: adenocarcinoma (AC) and squamous cell carcinoma (SCC). The five-year survival rate of patients with stage I NSCLC is two times higher than in patients with stage II and more than five times higher than in stage III−IV lung cancer patients. Currently, there are no informative blood biomarkers to diagnose early stages of NSCLC. The aim of this study was to evaluate complex determination of hyaluronic acid (HA), lymphocyte CXCR2 and granulocyte CXCR1 levels in the blood of patients with AC and SCC. Blood samples from 107 patients with SCC, 90 patients with AC, and 40 healthy people were used in this study. Concentration of HA in blood serum was determined by enzyme linked immunoassay. The level of CXCR2 and CXCR1 was determined by flow cytometry. Diagnostic parameters were determined by constructing mathematical models in the form of regression equations using the method of stepwise inclusion of predictors and subsequent ROC-analysis. Results of the study indicate that MFI CXCR1 in granulocytes, the proportion of lymphocytes expressing CXCR2 and concentration of HA in blood serum in stage I AC and SCC are significantly higher than in healthy people. The level of these indicators significantly increases at stage II of the disease compared to stage I and demonstrates further growth at its later stages. Based on obtained results, regression equations were created. These included: (i) MFI CXCR1 in granulocytes, the proportion of lymphocytes supplied with CXCR2 and the HA serum concentration in to detect stages I−II SCC (diagnostic sensitivity 95.7%, specificity 93.7%, threshold value 0.59) and stages III−IV SCC (diagnostic sensitivity 93.1%, specificity 93.3%, threshold value 0.64); (ii) the proportion of lymphocytes supplied with CXCR2, MFI CXCR1 in granulocytes and CYFRA 21-1 blood level, for detection of I−II stages of AC (sensitivity 91.3%, specificity 94.7%, threshold value 0.61); (iii) the proportion of lymphocytes expressing CXCR2 and CYFRA 21-1 blood level, for detection of AC stages III–IV (sensitivity 94.6%, specificity 91.3%, threshold value 0.15); (IV) the proportion of lymphocytes expressing CXCR2 and serum HA level to differentiate stage II SCC from stage I (sensitivity 94.4%, specificity 87.5%, threshold value 0.44) and II stage AC from stage I (sensitivity 88.5%, specificity 91.2%, threshold value 0.46).</p></div></div>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"16 1","pages":"45 - 53"},"PeriodicalIF":0.6,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4567763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurotransmitter Impairments in the Brain Induced by Acute Combined Intoxication of Rats with Ethanol and Morphine","authors":"I. M. Vialichko,&nbsp;S. V. Lelevich,&nbsp;V. V. Lelevich,&nbsp;E. M. Doroshenko,&nbsp;V. Yu. Smirnov","doi":"10.1134/S1990750822010103","DOIUrl":"10.1134/S1990750822010103","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>The levels of biogenic monoamines and their metabolites have been investigated in the hypothalamus, midbrain, and cerebellum of rats exposed to acute combined intoxication with morphine and ethanol. A single treatment of rats with ethanol and morphine caused neurotransmitter impairments in particular parts of the brain; they depended on the order of morphine and ethanol administration. The combined intoxication with ethanol and morphine led to signs of dopamine utilization only in the hypothalamus, regardless of the order of administration of psychoactive substances (PAS). During alcohol-morphine intoxication in the studied parts of the brain there was an increase in the level of metabolites of the serotonergic system. The combined action of the two surfactants largely corresponded to the effect of the last introduced substance in the midbrain and cerebellum. The combined administration of these PAS had a significant impact on parameters of the dopaminergic and serotonergic systems manifested by the processes of dopamine catabolism and a decrease in the norepinephrine and serotonin concentrations in the hypothalamus and these changes were not observed after ethanol or morphine administration alone.</p></div></div>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"16 1","pages":"66 - 73"},"PeriodicalIF":0.6,"publicationDate":"2022-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4567148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}