The Plant Journal最新文献

{"title":"Loss of CsCLV2 function causes dwarfism and determinates growth in cucumber","authors":"Lin Chen,&nbsp;Maomao Yun,&nbsp;Baoying Chen,&nbsp;Shuyan Xie,&nbsp;Wenrui Liu,&nbsp;Min Wang,&nbsp;Jinqiang Yan,&nbsp;Jinsen Cai,&nbsp;Songguang Yang,&nbsp;Qingwu Peng,&nbsp;Dasen Xie,&nbsp;Yu'e Lin,&nbsp;Biao Jiang","doi":"10.1111/tpj.70525","DOIUrl":"10.1111/tpj.70525","url":null,"abstract":"<div>\u0000 \u0000 <p>Cucumber (<i>Cucumis sativus</i> L.) is a globally important vegetable crop. Ideal plant architecture optimizes spatial utilization, enhances economic coefficient, and facilitates mechanized cultivation. In this study, we identified a dwarf mutant, <i>csdw3</i>, exhibiting reduced plant height, shortened internodes, and fewer internodes. Genetic analysis showed that this dwarf phenotype is controlled by a single recessive gene. Fine-mapping localized the causal locus to an 80 kb region on chromosome 1, where we discovered a 102 bp deletion in <i>CsCLV2</i>, a gene encoding a leucine-rich repeat receptor-like protein homologous to <i>Arabidopsis CLAVATA2</i>. CRISPR-Cas9-generated loss-of-function mutants recapitulated the dwarf phenotype, confirming the role of <i>CsCLV2</i> in plant height regulation. Histological examination revealed that <i>CsCLV2</i> disruption causes premature termination of shoot apical meristem (SAM) development, reducing both internode number and length. Protein interaction assays further demonstrated that <i>CsCLV2</i> associates with receptor-like kinase <i>CsCIK1</i> (<i>CLAVATA3 INSENSITIVE RECEPTOR KINASES 1</i>), indicating their cooperative function in the <i>CLV</i>-<i>WUS</i> signaling pathway to maintain meristem activity. Our findings uncover a regulator of plant height in cucumber and provide valuable genetic resources for breeding ideotypes optimized for yield and cultivation efficiency.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promiscuous potatoes: the genetic basis of wild potato compatibility","authors":"Gwendolyn K. Kirschner","doi":"10.1111/tpj.70529","DOIUrl":"10.1111/tpj.70529","url":null,"abstract":"&lt;p&gt;Few plants have shaped dinner plates and world history quite like the potato. Both the tetraploid cultivated potato (&lt;i&gt;Solanum tuberosum&lt;/i&gt; L.) and its wild tuber-bearing relatives belong phylogenetically to the &lt;i&gt;Solanum&lt;/i&gt; section &lt;i&gt;Petota&lt;/i&gt; (Yan et al., &lt;span&gt;2023&lt;/span&gt;). There are more than 100 recognized species of cultivated potatoes and their wild relatives. Like cultivated potatoes, many of these carry the A genome; they are primarily found in South America and are reproductively isolated from Mexican diploid species. The only diploid A-genome species occurring in Mexico is &lt;i&gt;Solanum verrucosum&lt;/i&gt; Schlechtendal, which also serves as a maternal ancestor of Mexican polyploid species (Hosaka et al., &lt;span&gt;2022&lt;/span&gt;). Interestingly, &lt;i&gt;S. verrucosum&lt;/i&gt; is also the only A-genome diploid potato species that is fully self-compatible.&lt;/p&gt;&lt;p&gt;William Behling, first author of the highlighted publication, has long been fascinated by crop wild relatives, and he was especially drawn to &lt;i&gt;S. verrucosum&lt;/i&gt;, which can serve as a bridge between the gene pool of cultivated and wild species because it can be fertilized by a broad range of wild &lt;i&gt;Solanum&lt;/i&gt; species (Behling et al., &lt;span&gt;2024&lt;/span&gt;). The more he worked with &lt;i&gt;S. verrucosum&lt;/i&gt;, the more he wanted to know why this species was able to do something that no other potato species seemed to be able to do—accept pollen from any male-fertile pollen donor.&lt;/p&gt;&lt;p&gt;In &lt;i&gt;S. verrucosum&lt;/i&gt;, self-compatibility has been attributed to the lack of S-ribonuclease (S-RNase) expression in the style (Eijlander et al., &lt;span&gt;2000&lt;/span&gt;). Together with other factors such as HT genes, the S-RNase has a cytotoxic effect on incompatible pollen tubes penetrating the style. However, knocking out the &lt;i&gt;S-RNase&lt;/i&gt; gene in &lt;i&gt;S. tuberosum&lt;/i&gt; is not sufficient to replicate the phenotypes observed in &lt;i&gt;S. verrucosum&lt;/i&gt; (Behling &amp; Douches, &lt;span&gt;2023&lt;/span&gt;). Additionally, there are examples of pollen rejection mechanisms that are independent of S-RNase in interspecific pollinations in tomato (Tovar-Méndez et al., &lt;span&gt;2017&lt;/span&gt;). Therefore, Behling, David Douches and colleagues set out to identify additional genetic factors that determine interspecific compatibility in &lt;i&gt;S. verrucosum&lt;/i&gt; (Behling et al., &lt;span&gt;2025&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;To create a F2 mapping population, segregating for the presence and absence of interspecific reproductive barriers (IRBs), the authors selected two &lt;i&gt;Solanum&lt;/i&gt; parents differing in their reproductive behaviour: DM1S1, a &lt;i&gt;S. tuberosum&lt;/i&gt; doubled monoploid that is effectively male sterile and has functional IRBs, but is able to accept pollen from &lt;i&gt;S. verrucosum&lt;/i&gt;; and MSJJ1813-2, a &lt;i&gt;S. verrucosum&lt;/i&gt; clonal selection that exhibits a high degree of male fertility, self-compatibility and lacks IRBs (Figure 1a).&lt;/p&gt;&lt;p&gt;To test for self-compatibility, the population was then allowed to self-pollinate. The authors first classified the phenotypes as self-compat","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70529","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRAnalyzer: a robust workflow for long non-coding RNA discovery using RNA-Seq","authors":"Shinde Nikhil,&nbsp;Habeeb Shaik Mohideen,&nbsp;Raja Natesan Sella","doi":"10.1111/tpj.70509","DOIUrl":"10.1111/tpj.70509","url":null,"abstract":"<div>\u0000 \u0000 <p>Long non-coding RNA (lncRNA) is a major transcript category that lacks protein-coding capabilities, with relatively low abundance and complex expression patterns. Distinguishing lncRNAs from protein-coding genes is a complex process involving multiple filtering steps. We developed an automated pipeline named LncRAnalyzer featuring retrained models for 60 species. This workflow aims to reduce the likelihood of obtaining protein-coding or partial protein-coding transcripts during lncRNA identification by utilizing eight distinct approaches. We conducted a 10-fold cross-validation of the sorghum models and training sets with their standard ones and other approaches using real-life RNA-Seq datasets and known lncRNA and CDS sequences of sorghum. The results showed that the sorghum models and training sets were outperformed. The pipeline output comprises upset plots illustrating the number of lncRNA/NPCTs identified by the approaches, commonly identified lncRNA and their classes, NPCTs, and expression count tables. A feature-level comparison and benchmarking analysis of LncRAnalyzer with four existing pipelines, namely, LncPipe, LncEvo, lncRNA-Annotation, and Plant-LncPipe, demonstrated that LncRAnalyzer is more comprehensive, easier to implement, and accurate in lncRNA predictions. This workflow also ascertains lncRNA origins from various Transposable Elements (TEs) in plants using TE annotations from APTEdb [http://apte.cp.utfpr.edu.br/]. LncRAnalyzer is publicly available on GitLab [https://gitlab.com/nikhilshinde0909/LncRAnalyzer.git] for academic users.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR159b, an epigenetic target of RSI1/FLD, negatively regulates systemic acquired resistance","authors":"Ranjan Kumar,&nbsp;Reena Saini,&nbsp;Deepjyoti Singh,&nbsp;Sujata Kaushik,&nbsp;Ashis Kumar Nandi","doi":"10.1111/tpj.70519","DOIUrl":"10.1111/tpj.70519","url":null,"abstract":"<div>\u0000 \u0000 <p>Plants retain memories of past infections to mount a robust defense in the form of systemic acquired resistance (SAR) during subsequent pathogen invasions. Primary infected tissues generate a group of compounds that serve as mobile signals for SAR development. Downstream processes subsequent to mobile signal perception are little known. Epigenetic regulator <i>reduced systemic immunity1/FLOWERING LOCUS D</i> (RSI1/FLD) is essential for activating SAR and functions downstream of signal perception in the systemic tissues. Here, we show that RSI1 negatively regulates the expression of miR159b, which in turn regulates the expression of a set of genes that control SAR development. RSI1 physically associates and contributes to SAR-associated demethylation of H3K4me2 and H3K4me3 at the <i>MIR159B</i> locus. Overexpression of miR159b suppresses SAR development, whereas SAR is exaggerated in <i>mir159ab</i> double mutants and target mimic expressing STTM159 lines. Through bioinformatics and expression analysis, we identified several targets of miR159, among which <i>SDG14</i>, <i>RD19A</i>, <i>MYB65</i>, <i>MYB33</i>, <i>MYB120</i>, <i>TPST</i>, <i>TIE4</i>, <i>CSD3</i>, and <i>PPDK</i> positively regulate SAR development, whereas <i>MYB97</i> and <i>MYB104</i> negatively regulate it. Altogether, our work identified a functional network of genes that activate and fine-tune SAR development in <i>Arabidopsis thaliana</i>.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The PtrRZFP4-PtrJAZ2 module regulates the jasmonic acid signaling pathway involved in the interaction between poplar and herbivorous insects","authors":"Fang He,&nbsp;Peng Yang,&nbsp;Yuan Zhang,&nbsp;Hao Li,&nbsp;Shu-Ying Wei,&nbsp;Shuang-Lian Deng,&nbsp;Jia-hui Liu,&nbsp;Ting Wang,&nbsp;Bo Li,&nbsp;Tiantian Lin","doi":"10.1111/tpj.70502","DOIUrl":"10.1111/tpj.70502","url":null,"abstract":"<div>\u0000 \u0000 <p>The plant hormone jasmonic acid (JA) is pivotal in regulating plant growth and defense mechanisms. Despite extensive research on the JA signaling network, the integration of other factors with JAZ (jasmonate ZIM domain) proteins to modulate JA signaling in response to diverse herbivore attacks remains unclear. In this study, we employed molecular biology and multi-omics techniques to identify an E3 ubiquitin ligase, PtrRZFP4. This E3 ubiquitin ligase exerts a positive regulatory effect on the JA signaling pathway, yet demonstrates differential responses to distinct insect types. We found that upon insect attack, the expression of <i>PtrRZFP4</i> transcripts is upregulated. In addition, PtrRZFP4 is able to interact with PtrJAZ2 and promote the ubiquitination and degradation of PtrJAZ2 protein. Furthermore, <i>PtrRZFP4</i> activates the jasmonic acid (JA) signaling pathway at both the transcriptional and metabolic levels, triggering the synthesis of a large number of secondary metabolites related to insect resistance, such as terpenoids and alkaloids. This ultimately enhances the plant's defense against insect herbivory. The larvae of the specialist insect showed a strong preference for feeding on the leaves of transgenic poplar overexpressing <i>PtrRZFP4</i> (<i>OX-PtrRZFP4</i>), which contain elevated levels of secondary metabolites. In contrast, the larvae of the generalist insect avoid leaves with increased secondary metabolite levels. However, <i>PtrJAZ2</i> exhibits an opposite function to <i>PtrRZFP4</i> in the resistance of poplar trees to different herbivores. Therefore, our study uncovers the significant role of the PtrRZFP4-PtrJAZ2 module in plant JA signaling and resistance to insect herbivory, highlighting its potential for biotechnological applications in improving herbivore resistance in forest trees.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond","authors":"Kangquan Yin,&nbsp;Chung-Jui Tsai","doi":"10.1111/tpj.70527","DOIUrl":"10.1111/tpj.70527","url":null,"abstract":"<p>Multiplex CRISPR editing has emerged as a transformative platform for plant genome engineering, enabling the simultaneous targeting of multiple genes, regulatory elements, or chromosomal regions. This approach is effective for dissecting gene family functions, addressing genetic redundancy, engineering polygenic traits, and accelerating trait stacking and <i>de novo</i> domestication. Its applications now extend beyond standard gene knockouts to include epigenetic and transcriptional regulation, chromosomal engineering, and transgene-free editing. These capabilities are advancing crop improvement not only in annual species but also in more complex systems such as polyploids, undomesticated wild relatives, and species with long generation times. At the same time, multiplex editing presents technical challenges, including complex construct design and the need for robust, scalable mutation detection. We discuss current toolkits and recent innovations in vector architecture, such as promoter and scaffold engineering, that streamline workflows and enhance editing efficiency. High-throughput sequencing technologies, including long-read platforms, are improving the resolution of complex editing outcomes such as structural rearrangements—often missed by standard genotyping—when targeting repetitive or tandemly spaced loci. To fully realize the potential of multiplex genome engineering, there is growing demand for user-friendly, synthetic biology-compatible, and scalable computational workflows for gRNA design, construct assembly, and mutation analysis. Experimentally validated inducible or tissue-specific promoters are also highly desirable for achieving spatiotemporal control. As these tools continue to evolve, multiplex CRISPR editing is poised to become a foundational technology of next-generation crop improvement to address challenges in agriculture, sustainability, and climate resilience.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12527382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin regulates tomato cold tolerance through SlPMTRs-SlCaM6-SlICE1 signaling cascade","authors":"Ying Liu,&nbsp;Shirui Jing,&nbsp;Congyang Jia,&nbsp;Zhe Ma,&nbsp;Jiawei Li,&nbsp;Qiuyu He,&nbsp;Chonghua Li,&nbsp;Yang-Dong Guo,&nbsp;Na Zhang","doi":"10.1111/tpj.70528","DOIUrl":"10.1111/tpj.70528","url":null,"abstract":"<div>\u0000 \u0000 <p>Cold stress severely impairs plants' growth and productivity by inducing oxidative damage and disrupting cellular signaling. While phytomelatonin (MT) enhances cold tolerance, the role of its specific receptors and the signaling transduction pathways remains unclear. This study has demonstrated that the phytomelatonin receptors SlPMTR1/2 are essential for phytomelatonin protection against cold injury in tomatoes when treated at 4°C for 48 h. Upon sensing 10 μM melatonin, SlPMTR1/2 mitigated oxidative damage through enhancing activities of antioxidant enzyme activities (superoxide dismutase, peroxidase, catalase, ascorbate peroxidase), thereby reducing levels of reactive oxygen species and malondialdehyde accumulation. Meanwhile, SlPMTR1/2 physically interacted with calmodulin SlCaM6, recruiting it to the plasma membrane and reducing its nuclear localization. This sequestration alleviated SlCaM6's inhibition of the transcription factor SlICE1 in the nucleus. Consequently, released SlICE1 activated the expression of <i>SlCBF1</i> and downstream <i>COR</i> genes. Furthermore, SlCBF1 directly upregulated the expression of cyclic nucleotide-gated channels 2 (<i>SlCNGC2</i>), promoting extracellular Ca²⁺ influx upon cold shock—a response amplified by MT in a SlPMTR1/2-dependent manner. This enhanced Ca²⁺ signaling reinforces cold tolerance. Collectively, we have unveiled a dual-pathway signaling cascade where SlPMTR1/2 orchestrated tomato cold adaptation by enhancing antioxidant enzyme activities and interacting with SlCaM6 to activate the SlICE1-SlCBF1/SlCNGC2 transcriptional module to amplify Ca²⁺-mediated cold responses.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CmCBF4 crosstalk with H2O2 signal involved in trehalose-promoted cold tolerance of melon seedlings","authors":"Yuqing Han,&nbsp;Hongyi Zhang,&nbsp;Yujie Zhang,&nbsp;Lin Dong,&nbsp;Haiming Li,&nbsp;Jiahui Tian,&nbsp;Kexin Lu,&nbsp;Tao Liu,&nbsp;Hongyan Qi","doi":"10.1111/tpj.70520","DOIUrl":"10.1111/tpj.70520","url":null,"abstract":"<div>\u0000 \u0000 <p>Cold stress severely inhibits the normal growth of melon (<i>Cucumis melo</i> var. <i>makuwa</i> Makino) seedlings. At low temperature, the moderate increase of apoplastic hydrogen peroxide (H₂O₂) level and subsequent intracellular signal transduction are important to plant cold response. Our previous studies showed that trehalose (Tre) could activate H₂O₂ signal and improve cold tolerance of melon seedlings. However, the specific mechanism is not completely clear. Here, we found that Tre-promoted C-repeat binding factor 4 (CmCBF4) could combine with the promoter of <i>respiratory burst oxidase homologs D</i> (<i>CmRBOHD</i>) to enhance the production of apoplastic H₂O₂. Further studies suggested that CmCBF4 could also activate the transcription of <i>plasma membrane intrinsic protein 2;3</i> (<i>CmPIP2;3</i>), which has H₂O₂ transport capability in melon seedlings. In addition, abscisic acid-responsive element (ABRE)-binding factor 2 (CmABF2) and CmCBF4 could promote the transcription of each other to increase the expression of <i>CmPIP2;3.</i> Silencing <i>CmCBF4</i> significantly reduced Tre-promoted cold tolerance and apoplastic H₂O₂ production and transport. In summary, this study reveals that CmCBF4 regulates Tre-promoted cold tolerance in melon seedlings by promoting the production and transport of apoplastic H₂O₂ signal.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rice-specific miRNA miR24584 enhances JA signaling by silencing OsJAZ13 to boost rice blast resistance","authors":"Xin-Xian Liu,&nbsp;Nuo-Wen Zhang,&nbsp;Chao-Rong Guo,&nbsp;Peng-Yang Li,&nbsp;Ya-Xin Wang,&nbsp;Hao Su,&nbsp;Yong Zhu,&nbsp;Wen-Rui Ren,&nbsp;Chu-Tian Tan,&nbsp;Chen Liu,&nbsp;Xue-Mei Yang,&nbsp;He Wang,&nbsp;Jun Shi,&nbsp;Guo-Bang Li,&nbsp;Zhi-Xue Zhao,&nbsp;Ji-Wei Zhang,&nbsp;Jing Fan,&nbsp;Yan Li,&nbsp;Yan-Yan Huang,&nbsp;Wen-Ming Wang","doi":"10.1111/tpj.70521","DOIUrl":"10.1111/tpj.70521","url":null,"abstract":"<div>\u0000 \u0000 <p>Many conserved miRNAs are reported to function in plant immunity, while the discovery and functional characterization of additional immunity-related miRNAs remain an active area of research. In this study, we identified miR24584, a 24-nucleotide miRNA, as a critical positive regulator of rice immunity against <i>Magnaporthe oryzae</i>. The accumulation of miR24584 exhibits dynamic patterns upon pathogen challenge, with upregulation in resistant rice accessions and downregulation in susceptible accessions. The transgenic lines overexpressing miR24584 conferred resistance against diverse <i>M. oryzae</i> isolates, whereas suppression through target mimicry rendered plants susceptible. Furthermore, miR24584 targets the 3′ untranslated region of <i>OsJAZ13</i>, a transcriptional repressor of jasmonate (JA) signaling, and suppresses its expression. Transgenic overexpression of <i>OsJAZ13</i> caused seedling lethality, whereas CRISPR/Cas9-mediated knockout lines displayed blast resistance accompanied by elevated JA accumulation and upregulated expression of JA-responsive marker genes. Intriguingly, following <i>M. oryzae</i> inoculation and chitin treatment, <i>osjaz13</i> mutant lines displayed significantly increased callose deposition similarly to the transgenic lines overexpressing miR24584. Altogether, our results establish the miR24584-<i>OsJAZ13</i> regulatory module that activates JA signaling to enhance physical barrier formation to optimize blast resistance. This work expands the known repertoire of immunity-associated miRNAs.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethylene enhances cold resistance through GhDREB1/CBF in cotton (Gossypium hirsutum L.)","authors":"Yaxin Dong,&nbsp;Huijuan Ma,&nbsp;Yanhui Shen,&nbsp;Pengzhen Li,&nbsp;Changwei Ge,&nbsp;Qian Shen,&nbsp;Jinglin Li,&nbsp;Ruihua Liu,&nbsp;Siping Zhang,&nbsp;Shaodong Liu,&nbsp;Chaoyou Pang","doi":"10.1111/tpj.70517","DOIUrl":"10.1111/tpj.70517","url":null,"abstract":"<div>\u0000 \u0000 <p>Although the role of ethylene in plant growth and development has been widely studied, its regulatory effect on cold tolerance varies among crops, and the mechanisms underlying this variability remain unclear. We used weighted gene co-expression network analysis (WGCNA) to analyse cotton transcriptome changes under low-temperature stress. Differentially expressed genes were significantly enriched in those related to ethylene signalling pathways, suggesting their potential role in cold stress responses. The positive effect of ethylene on cold tolerance in cotton was demonstrated by the effects of exogenously applied ethylene precursor 1-aminocyclopropane-1-carboxylic acid and ethylene synthesis inhibitor α-aminoisobutyric acid. Using CRISPR/Cas9, virus-induced gene silencing, as well as overexpression in tobacco, we obtained evidence indicating that the ethylene synthesis gene <i>GhACO1</i> enhanced plant cold tolerance. Transcriptome analysis showed that the C-repeat/DRE binding factor (GhDREB1/CBF) was highly expressed in cotton and significantly upregulated by low-temperature stress. The CUT&amp;Tag approach suggested that GhDREB1 binds to the <i>GhACO1</i> promoter. The direct regulation of <i>GhACO1</i> by GhDREB1 was further confirmed through luciferase reporter gene and yeast one-hybrid detection. These results suggest that <i>GhACO1</i> enhances cold tolerance of cotton via the CBF-dependent pathway. Transgenic cotton plants overexpressing GhDREB1 exhibited elevated GhACO1 expression and improved cold resistance, further supporting the regulatory role of GhDREB1. Our results revealed that <i>GhACO1</i>-mediated ethylene synthesis is modulated by GhDREB1, which positively regulates cold tolerance in cotton. These findings provide valuable insights into the molecular mechanisms underlying cold tolerance in cotton and lay the foundation for improving crop resilience to low-temperature stress.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}