The Plant Journal最新文献

{"title":"Altered plastidial fatty-acid condensation is associated with changes in membrane composition and postembryonic leaf development in Arabidopsis","authors":"Evan R. Angelos,&nbsp;Amirreza R. Bagh-abrishami,&nbsp;María Fernanda Gómez-Méndez,&nbsp;Katharina Gutbrod,&nbsp;Andrea A. Zanini,&nbsp;De Fan,&nbsp;Hee-Seung Choi,&nbsp;Liping Zeng,&nbsp;Jiaxuan Cheng,&nbsp;Bailong Zhang,&nbsp;Meng Chen,&nbsp;Tessa M. Burch-Smith,&nbsp;Peter Dörmann,&nbsp;Katayoon Dehesh","doi":"10.1111/tpj.71243","DOIUrl":"https://doi.org/10.1111/tpj.71243","url":null,"abstract":"<p>Membrane lipid composition is fundamental to organelle structure and environmental adaptation, yet how plastidial fatty acid metabolism influences developmental plasticity remains poorly understood. We examined the effects of sustained elevation of the plastidial fatty-acid enzymes KASII (β-ketoacyl-ACP synthase II) and SSI2 (Δ9 stearoyl-ACP desaturase 2) in <i>Arabidopsis thaliana</i> using lipidomic, ultrastructural, cellular, molecular, and physiological analyses. KASII overexpressing plants (KASIIox), but not SSI2 overexpressing plants (SSI2ox), increased carbon 18-containing lipid species and exhibited reduced plastidial galactolipids together with increased extraplastidial phospholipids. KASIIox plants displayed enlarged chloroplasts with disrupted thylakoid organization, impaired palisade differentiation, altered mesophyll patterning, and reduced seed coat mucilage. Under low light, KASIIox plants failed to exhibit the characteristic petiole elongation observed in control and SSI2ox plants, accompanied by altered phyB and PIF4 protein abundance. Exogenous gibberellin intensified, whereas inhibition of gibberellin biosynthesis partially alleviated KASIIox leaf and cellular phenotypes. Together, these findings associate sustained KASII elevation with changes in membrane lipid composition, chloroplast architecture, light-responsive growth, and postembryonic leaf development, supporting a link between plastidial fatty-acid metabolism and developmental responses to light and gibberellin availability.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71243","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149356154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An S-domain receptor-like kinase regulates auxin distribution and root gravitropism via MPK signaling in Arabidopsis","authors":"Myint Myint San,&nbsp;Caoli Zhang,&nbsp;Yakun Peng,&nbsp;Meng Chen,&nbsp;Michelle Gallei,&nbsp;Lianghanxiao Sun,&nbsp;Mengjuan Kong,&nbsp;Jiří Friml,&nbsp;Shutang Tan","doi":"10.1111/tpj.71206","DOIUrl":"10.1111/tpj.71206","url":null,"abstract":"<p>Root system architecture (RSA) varies across environments and plays essential roles in plant adaptive growth. Auxin and its polar transport have been found to be key players in the regulation of RSA. However, the molecular mechanisms underlying the regulation of RSA in fluctuating environments remain unclear. Using a forward genetic screen for root mutants under gravitropism interference conditions in the model plant <i>Arabidopsis thaliana</i>, we revealed that <i>HYPERSENSITIVE TO IBUPROFEN1</i> (<i>HIB1</i>) regulates root gravitropic growth and RSA by modulating auxin distribution in Arabidopsis. The <i>hib1</i> seedlings presented defects in primary root elongation, lateral root organogenesis, and the root gravitropic response because of abnormal auxin distribution. Notably, the gravitropic defects of <i>hib1</i> roots were correlated with increased MPK6 activity and could be rescued by loss-of-function <i>mpk6</i> mutation. Furthermore, cell biological results revealed that HIB1 regulated PIN protein polar distribution and abundance at the plasma membrane, indicating that HIB1 is involved in polar auxin transport and the subsequent auxin response. Indeed, genetic perturbation of <i>HIB1</i> results in impaired gravitropic root growth. Our work revealed that the HIB1-auxin module regulates root gravitropism and RSA, providing insights into the molecular mechanisms underlying adaptive root growth.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71206","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149340081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telomere-to-telomere genome assembly of the endangered Jacktree (Sinojackia xylocarpa Hu) reveals its centromeric landscape and evolutionary dilemma as a small population","authors":"Si-Di Liang,&nbsp;Si-Si Zheng,&nbsp;Lei Wu,&nbsp;Ang Liu,&nbsp;Hong-Hu Meng,&nbsp;Yun Jiang,&nbsp;Zheng-Wei Wang,&nbsp;Bin-Jie Ge,&nbsp;Wen-Li Zou,&nbsp;Qian Wan,&nbsp;Xi-Ling Dai,&nbsp;Gregor Kozlowski,&nbsp;Nai-Wei Li,&nbsp;Xiao-Ling Yan,&nbsp;Yi-Gang Song","doi":"10.1111/tpj.71186","DOIUrl":"10.1111/tpj.71186","url":null,"abstract":"<p>The endangered status of many plant species is often attributed to a decline in fitness, which drives a sustained decrease in population size under natural conditions. However, the role of highly repetitive genomic regions in the decline of endangered plants remains poorly understood. Here, we present the gap-free telomere-to-telomere (T2T) reference genome for the endangered Jacktree (<i>Sinojackia xylocarpa</i> Hu). It has a genome size of 961.94 Mb, an N50 of 78.05 Mb, and 36 446 predicted protein-coding genes. Using CENH3 chromatin immunoprecipitation sequencing (ChIP-seq), we identified centromeric regions across all 12 chromosomes and revealed that <i>S. xylocarpa</i> centromeres are composed of diverse transposable element (TE) families, with long terminal repeat (LTR) retrotransposons and LINE elements as major components. Notably, the right arm of chromosome 1 exhibited ectopic CENH3 enrichment and a TE accumulation similar to that of centromeric regions, suggesting the formation of a centromere-like chromatin domain. In addition, we detected inversion polymorphisms and inversion heterozygotes in natural populations, predominantly concentrated on the same chromosomal arm. These features may be associated with meiotic abnormalities. Using a high-quality T2T genome assembly, we identified a pattern of genomic erosion facing these small populations characterized by local suppression of recombination linked LTR proliferation and by the accumulation of deleterious mutations in DNA repair genes. This genomic erosion creates an evolutionary dilemma that impairs the purging of deleterious alleles, ultimately threatening long-term adaptability and survival. This study highlights the utility of T2T genome assembly for the conservation of endangered plants.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13732550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis thaliana BOLA proteins, new actors in plant growth and iron homeostasis","authors":"Maël Taupin-Broggini,&nbsp;Anaïs Rodriguez-Euzet,&nbsp;Brigitte Touraine,&nbsp;Nathalie Berger,&nbsp;Julie Bals,&nbsp;Daniel Marino,&nbsp;Christian Dubos,&nbsp;Florence Vignols","doi":"10.1111/tpj.71196","DOIUrl":"10.1111/tpj.71196","url":null,"abstract":"<p>The universal iron–sulfur (Fe-S) clusters embedded in proteins support numerous fundamental biological processes. However, many proteins involved in Fe-S assembly machineries remain poorly characterized in plants. Here, we revealed that <i>BOLA1</i> and <i>BOLA4,</i> two members of the BOLA family previously described as Fe-S assembly factors in various organisms, are critical growth regulators that play a role in Fe homeostasis in <i>Arabidopsis thaliana</i>. Expression analyses showed that <i>BOLA1</i> and <i>BOLA4</i> are ubiquitously expressed across most Arabidopsis tissues. Using protoplast transfection assays, we also demonstrated that both BOLA-GFP fusion proteins localize to chloroplasts and mitochondria. Mutant analyses revealed that while single <i>bola1</i> or <i>bola4</i> knock-out mutants are phenotypically indistinguishable from the wild type, double <i>bola1 bola4</i> mutants display severe growth defects, including early growth arrest of the primary root. Notably, this phenotype was rescued by grafting a wild-type root system onto the <i>bola1 bola4</i> aerial part. Fe quantification, Perls staining, and RT-qPCR analyses further demonstrated that <i>bola1 bola4</i> double mutants exhibit altered Fe distribution and Fe-homeostasis-related gene expression. Overall, our findings show that <i>BOLA1</i> and <i>BOLA4</i> act redundantly to maintain primary root growth and rosette development and are essential players in Fe signaling and partitioning in Arabidopsis.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71196","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GmRVE8a, a circadian clock-associated gene, negatively regulates flowering and maturity in soybean","authors":"Shibo Sun,&nbsp;Maoxiang Yang,&nbsp;Tingting Wu,&nbsp;Bingjun Jiang,&nbsp;Xuegang Sun,&nbsp;Hongchang Jia,&nbsp;Xiangyu Yao,&nbsp;Yanhui Sun,&nbsp;Qimeng Li,&nbsp;Peiguo Wang,&nbsp;Liwei Jiang,&nbsp;Chao Qin,&nbsp;Shan Yuan,&nbsp;Zhaoming Qi,&nbsp;Shi Sun,&nbsp;Huihui Li,&nbsp;Tianfu Han,&nbsp;Baiquan Sun","doi":"10.1111/tpj.71205","DOIUrl":"10.1111/tpj.71205","url":null,"abstract":"<p>Precise regulation of flowering time and maturity is critical for achieving high and stable soybean yields, and the circadian clock plays a central role in coordinating these developmental transitions. Soybean is a typical short-day crop with a pronounced photoperiodic response; however, the molecular mechanisms linking circadian regulation to photoperiod-dependent flowering and maturity remain incompletely understood. Here, we integrated genome-wide association analysis with deep learning approaches using 257 soybean germplasm accessions spanning 14 maturity groups and identified <i>GmRVE8a</i>, a homolog of the Arabidopsis circadian clock gene <i>REVEILLE</i>, as a strong candidate locus associated with soybean maturity across two field environments. Haplotype analysis demonstrated that accessions carrying the <i>GmRVE8a</i>^<i>H1</i> haplotype consistently flowered and matured significantly later than those carrying <i>GmRVE8a</i>^<i>H2</i>. Functional validation revealed that knockout of <i>GmRVE8a</i> markedly accelerated flowering and maturity, whereas overexpression of <i>GmRVE8a</i> significantly delayed both processes. qRT-PCR assays further suggested that <i>GmRVE8a</i> may repress flowering and delay maturity by promoting the expression of <i>GmFT4</i>, a flowering-inhibitory member of the <i>FT</i> family, and is unlikely to act by directly regulating <i>E1</i> expression. Collectively, our findings suggest <i>GmRVE8a</i> as a circadian clock-associated negative regulator of soybean flowering and maturity, and highlight it as a promising candidate for molecular breeding aimed at improving adaptation to diverse photoperiodic environments.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13732551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population genomics of heterosporous lycophytes: allopatric speciation and historical persistence through paleoclimatic fluctuations","authors":"TongXin Ye,&nbsp;Ran Wei,&nbsp;JiGao Yu,&nbsp;NingXin Wang,&nbsp;SiQi Liang,&nbsp;XianChun Zhang","doi":"10.1111/tpj.71179","DOIUrl":"10.1111/tpj.71179","url":null,"abstract":"<p>Speciation is the fundamental process driving the origin of earth biodiversity. While speciation mechanisms have been revealed in many plant taxa, the evolutionary dynamics of heterosporous lycophytes remain largely unexplored. Here, we used the <i>Selaginella sanguinolenta</i> group, comprising seven montane species in eastern Asia, as a model system. We generated a high-quality, chromosome-level reference genome for <i>S</i>. <i>aitchisonii</i>, conducted whole-genome resequencing of 191 individuals across 49 populations, and integrated population genomic, phylogenomic, morphological, and ecological analyses. Our results revealed that strong geographic isolation, driven by Miocene paleogeographic events, led to high genetic differentiation and restricted interspecific gene flow. The unique combination of heterospory and independent gametophyte generations might exacerbate extreme inbreeding, further impeding the ability of these lineages to surmount geographical barriers. Ecological niche divergence indicated that these species experience heterogeneous selection pressures; moreover, evidence of selective sweeps, leaf arrangement variation, and functional gene divergence indicates that natural selection gives rise to new species with environmentally adaptive traits. Demographic reconstructions revealed a shared trajectory of expansion followed by contraction into bottlenecks from the late Miocene to the Pliocene. Montane refugia likely played a role in species persistence, preserving genetic diversity for the postglacial expansions observed in four species since the Last Interglacial. We conclude that the <i>S. sanguinolenta</i> group underwent allopatric speciation since the Miocene and successfully persisted through severe Quaternary climatic fluctuations, which provides novel insights based on population genomic analysis into the evolution of early-diverging vascular plants.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two nearly complete genomes reveal the molecular mechanism underlying the significant fruit quality differences caused by bud sport between yellow and white-fleshed loquat (Eriobotrya japonica L.)","authors":"Ke Zhao,&nbsp;Jinquan Chao,&nbsp;Yangang Pei,&nbsp;Tao Lang,&nbsp;Guoliang Jiang,&nbsp;Shuxia Sun,&nbsp;Hongjiang Xie,&nbsp;Jing Li,&nbsp;Meiyan Tu,&nbsp;Dong Chen,&nbsp;Mingchun Liu,&nbsp;Ronggao Gong,&nbsp;Haiyan Song","doi":"10.1111/tpj.71212","DOIUrl":"10.1111/tpj.71212","url":null,"abstract":"<p>Bud sport is a common and stable somatic variation in perennial fruit trees. Loquat (<i>Eriobotrya japonica</i> L.) is an evergreen fruit tree that can be categorized into yellow-fleshed and white-fleshed types based on the flesh color. To uncover the genetic basis, we herein assembled two nearly complete genomes for a yellow-fleshed ‘Dongting’ loquat (TBY) and its white-fleshed bud sport (TBW). The final sizes of assemblies were 773.29 Mb and 728.86 Mb with contig N50 of 31.87 Mb and 28.63 Mb for TBY and TBW, respectively. Comparative genomics analysis indicated that the heterozygosity ratio of TBY was nearly two-fold higher than that of TBW (0.53% vs. 0.27%). Integrated multi-omics and molecular marker detection results revealed that homozygosity of the <i>EjPSY2A</i>^<i>d</i>/<i>EjPSY2A</i>^<i>d</i> allele is the cause of the change in flesh color from yellow to white in TBY. Additionally, <i>EjSUSs</i> (<i>−1</i>, <i>−2</i>, <i>−3</i>, <i>−12</i>) and <i>EjSPS3</i> might be the key genes responsible for the significant accumulation of hexoses and sucrose, respectively, in the flesh of TBY and TBW. Furthermore, high expression of <i>EjC4H8</i> and <i>Ej4CLs</i> (<i>−7</i>, <i>−10</i>, <i>−20</i>) in TBY might lead to the accumulation of more lignin in its flesh. Taken together, these findings provide valuable insights into the molecular mechanism differences between yellow- and white-fleshed loquat and highlight the significance of bud sport as a valuable approach for generating novel germplasm resources in fruit trees.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71212","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bringing seed barriers into focus: Optimized clearing and histological staining","authors":"Fabien Nirina Ramamonjy,&nbsp;Luca Piccinini,&nbsp;Vanessa Reyes Loaiza,&nbsp;Hongjun Meng,&nbsp;Robertas Ursache","doi":"10.1111/tpj.71209","DOIUrl":"https://doi.org/10.1111/tpj.71209","url":null,"abstract":"<p>Plant reproduction relies on tightly coordinated developmental programs that integrate environmental and endogenous signals. Within seeds, lipid-based extracellular polymers such as cutin and suberin are deposited at defined apoplastic interfaces, where they function as selectively permeable barriers. These structures are actively remodeled by hormonal and metabolic cues to regulate hydration, nutrient exchange, and stress responses. Although the genetic and biochemical pathways underlying these barriers are increasingly well understood, their spatial organization and developmental dynamics remain difficult to investigate because they are embedded within optically dense and multilayered tissues. Existing clearing approaches have substantially improved visualization of internal seed structures, yet systematic whole-mount imaging of multiple lipid-based barriers across development, particularly in combination with fluorescent reporters, remains challenging. To address this, we developed an optimized pipeline that enables rapid and efficient seed tissue clearing and selective visualization of polyaliphatic barriers across four key seed interfaces: the seed surface, seed coat suberin, cuticle at the endosperm/seed coat boundary, and embryo surface cuticle. The method preserves the spatial context of these barriers within intact seeds and enables their co-visualization with fluorescence-based marker lines. Using a common whole-mount imaging framework across successive developmental stages, we follow changes in the spatial organization of distinct barrier domains. The approach is also applicable to more complex seeds, such as those of <i>Camelina sativa,</i> and can be extended to other anatomically complex plant organs by combining the clearing and staining workflow with vibratome sectioning.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71209","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149311440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-locus genetic basis of stomatal density and size in maize revealed by integrated QTL and GWAS","authors":"Yongtian Qin,&nbsp;Meng Qiu,&nbsp;Chuyang Wang,&nbsp;Zeyuan Yang,&nbsp;Xurui Ma,&nbsp;Zhaoyi Pan,&nbsp;Lingling Sun,&nbsp;Tingting Wu,&nbsp;Yuqi Huang,&nbsp;Hongqiu Wang,&nbsp;Qingqian Zhou,&nbsp;Jihua Tang,&nbsp;Zhiyuan Fu","doi":"10.1111/tpj.71187","DOIUrl":"https://doi.org/10.1111/tpj.71187","url":null,"abstract":"<p>Stomatal patterning is a fundamental aspect of plant photosynthetic performance and water-use efficiency, yet its genetic basis in C₄ grasses such as maize remains poorly understood. Here, we integrated linkage analysis across three recombinant inbred line populations and a genome-wide association study in a diverse maize panel to dissect the genetic architecture of six stomatal traits. This approach identified 33 quantitative trait loci and 51 association loci for 6 stomatal traits and revealed a significant negative genetic correlation between stomatal density (SD) and size, with multiple genomic intervals showing co-localization of effects on both trait categories. By integrating reported cell-type-specific transcriptomic data, we prioritized high-confidence candidate genes, including conserved stomatal regulators as well as previously uncharacterized candidates involved in processes such as cell wall biosynthesis. Functional analyses of EMS-induced mutants for candidates <i>ZmbHLH140</i> and <i>ZmCESA6</i> demonstrated that loss of <i>ZmbHLH140</i> reduces SD, whereas mutation of <i>ZmCESA6</i> has the opposite effect. In addition, the signature of selection at the <i>ZmbHLH140</i> locus is consistent with a role for stomatal regulation during maize domestication. Together, our results establish a comprehensive genetic framework for stomatal development in maize, highlighting both conserved and grass-specific mechanisms that shape stomatal patterning and offering a foundation for future functional studies of stomatal development in cereals.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71187","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149311311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The SlHYL1-SlRBP1 module integrates gibberellin metabolism to promote plant height in tomato (Solanum lycopersicum)","authors":"Xingxing Zhang,&nbsp;Miao Chai,&nbsp;Linjing Cao,&nbsp;Shengbo Chen,&nbsp;Kun Zhao,&nbsp;Jiazhen Han,&nbsp;Ning Li,&nbsp;Hongjiao Zhao,&nbsp;Shengjun Feng,&nbsp;Bo Wang,&nbsp;Shengze Zhang,&nbsp;Yumeng Li,&nbsp;Mingze Xu,&nbsp;Guodong Ren,&nbsp;Shuai Gao","doi":"10.1111/tpj.71210","DOIUrl":"https://doi.org/10.1111/tpj.71210","url":null,"abstract":"<p>Plant height is an essential agronomic trait that significantly influences crop yield and quality. The HYPONASTIC LEAVES 1 (HYL1), a core component of microRNA biogenesis, remains poorly understood in terms of its regulatory mechanisms on agronomic traits in tomato (<i>Solanum lycopersicum</i>, Sl). In this study, we demonstrated that CRISPR/Cas9-mediated knockout of <i>SlHYL1</i> caused dwarfism, and partial suppression of <i>SlHYL1</i> in tomato cultivar “Ailsa Craig” (AC) via RNA interference (RNAi) increased internode number but reduced internode length, resulting in a semi-dwarf phenotype, confirming the role of SlHYL1 in regulating plant height. Cytological analysis showed that <i>SlHYL1</i> suppression decreased both cell number and size within internodes. Yeast two-hybrid screening identified that SlHYL1 interacted with another tomato RNA-binding protein 1 (SlRBP1). Knockdown of <i>SlRBP1</i> resembled the <i>SlHYL1-RNAi</i> semi-dwarf phenotype. Further studies revealed that the SlHYL1-SlRBP1 module additively promotes the expression of gibberellin (GA) biosynthetic genes, thereby facilitating GA accumulation. Additionally, the dwarf phenotypes of both <i>SlHYL1-RNAi</i> and <i>SlRBP1-RNAi</i> plants were partially rescued by exogenous GA₃ application. Notably, unlike SlHYL1, SlRBP1 did not affect the biogenesis of most miRNAs. In summary, our findings demonstrate that the SlHYL1-SlRBP1 module regulates tomato plant height by enhancing GA accumulation.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149311318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}