The Plant Journal最新文献

RGIs-mediated root apical meristem development is essential for root hydrotropic response in Arabidopsis thaliana rgis介导的根尖分生组织发育是拟南芥根系嗜水响应的关键

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-06-25 DOI: 10.1111/tpj.70273

Yang Ou, Jingxia Yuan, Yanqin Ma, Xiaopeng Li, Haoyong Zhang, Jia Li

{"title":"RGIs-mediated root apical meristem development is essential for root hydrotropic response in Arabidopsis thaliana","authors":"Yang Ou, Jingxia Yuan, Yanqin Ma, Xiaopeng Li, Haoyong Zhang, Jia Li","doi":"10.1111/tpj.70273","DOIUrl":"https://doi.org/10.1111/tpj.70273","url":null,"abstract":"<div>\u0000 \u0000 Hydrotropism refers to the tendency of roots which prefer to grow towards a region with more water availability. We previously detected higher responses of cytokinins on the drier sides of Arabidopsis root tips, which lead to more cell division activity there and the growth of roots towards higher water potential areas. To further verify the significance of cell division activity in controlling root hydrotropism, we analyzed the hydrotropic responses of a number of well-characterized short root mutants with reduced root apical meristem activities. The mutants used were from a RGF1-RGIs signaling pathway, including a quadruple and a quintuple mutant for the receptors of an RGF1 peptide hormone, rgi1234 and rgi12345, a tyrosyl protein sulfotransferase mutant tpst, and a double mutant for two transcription factors, plt1 plt2. All these mutants showed a greatly reduced or even undetectable hydrotropic response. The expression levels of TCSn::GFP, a reporter system for monitoring the response of cytokinins, were drastically down-regulated in the root tips of rgi1234, rgi12345, tpst, and plt1 plt2. The introduction of pRGI2::MKK4DD, a construct encoding a constitutively active MKK4, can significantly rescue not only the short root phenotype but also the defective responses of cytokinins and hydrotropism in rgi12345. Interestingly, the hydrotropic response of tie1 tie2, a short root double mutant with normal cell division activity in the root apical meristem but defects in the elongation region, is relatively normal. Our results substantiate the key roles of the RGF1-RGIs signaling pathway and cell division activity in determining root hydrotropism. These analyses also reveal the crosstalk between the RGF1-RGIs signaling pathway and the responses of cytokinins, which are worth being further investigated in the future.\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative screening for mutants affected in seed oil/protein allocation identifies TRANSPARENT TESTA7 as a regulator of oil accumulation 对影响种子油/蛋白质分配的突变体的创新筛选确定了TRANSPARENT TESTA7作为油积累的调节剂

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-06-25 DOI: 10.1111/tpj.70269

Alain Lécureuil, Massimiliano Corso, Stéphanie Boutet, Sophie Le Gall, Regina Niñoles, Jose Gadea, Philippe Guerche, Sophie Jasinski

{"title":"Innovative screening for mutants affected in seed oil/protein allocation identifies TRANSPARENT TESTA7 as a regulator of oil accumulation","authors":"Alain Lécureuil, Massimiliano Corso, Stéphanie Boutet, Sophie Le Gall, Regina Niñoles, Jose Gadea, Philippe Guerche, Sophie Jasinski","doi":"10.1111/tpj.70269","DOIUrl":"https://doi.org/10.1111/tpj.70269","url":null,"abstract":"Brassicaceae species mainly accumulate oil and protein in their seeds, which are essential to human life as a source of food, but also as animal feed and resources for green chemistry. To date, Brassicaceae crops such as rapeseed have been selected mainly for their oil content. However, there is a growing interest in their seed protein content. A strong negative correlation between oil and protein content makes it difficult to increase both compounds simultaneously. In this study, an Arabidopsis thaliana homozygous EMS mutant library was screened for seed oil and protein content by near-infrared spectroscopy with the aim of identifying mutants with impaired oil/protein correlation. The mutant most affected in this correlation was found to be in the TRANSPARENT TESTA7 gene, which is involved in the flavonoid biosynthetic pathway. Analysis of different mutants in the flavonoid pathway revealed that the tt7 mutants were the only ones to show such a significant reduction in seed oil content, highlighting a phenotype never described before for the tt7 mutants and suggesting a specific role for TT7 in the interplay between the oil and flavonoid biosynthetic pathways. Untargeted metabolomic analysis allowed the identification of metabolic features that are highly accumulated and specific to tt7 seeds compared to the other genotypes and genetic analysis established that the accumulation of kaempferol-3-O-rhamnoside seems to be responsible for the seed oil reduction in tt7 mutants.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70269","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NF-YCs modulate RNA polymerase II-mediated transcription to regulate FLM expression NF-YCs通过RNA聚合酶ii介导的转录调节FLM的表达

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-06-25 DOI: 10.1111/tpj.70293

Xiani Yao, Ao Chen, Mingyang Jian, Xiaoming Li, Xu Liu, Xingliang Hou, Chunyu Zhang, Kangjia Li

{"title":"NF-YCs modulate RNA polymerase II-mediated transcription to regulate FLM expression","authors":"Xiani Yao, Ao Chen, Mingyang Jian, Xiaoming Li, Xu Liu, Xingliang Hou, Chunyu Zhang, Kangjia Li","doi":"10.1111/tpj.70293","DOIUrl":"https://doi.org/10.1111/tpj.70293","url":null,"abstract":"<div>\u0000 \u0000 The proper transition to flowering is a critical process for the success of plant reproduction and must be highly orchestrated. Nuclear Factor-Y subunit C (NF-YCs), which are closely related to histone H2A, exert diverse chromatin-mediated regulation over plant development events including flowering. However, the mechanisms by which NF-YCs regulate RNA polymerase II (Pol II) during gene transcription remain elusive. Here, we demonstrate that NF-YCs physically interact with EARLY FLOWERING 7 (ELF7), a core component of RNA Pol II-associated factor 1 complex (PAF1c), both in vitro and in vivo. We show that NF-YCs regulate flowering in an ELF7-dependent manner by repressing the expression of the floral repressor FLOWERING LOCUS M (FLM). Further analyses reveal that NF-YCs antagonize the binding of ELF7 to FLM chromatin, thereby suppressing ELF7-mediated RNA Pol II transcription at the FLM locus. Collectively, our findings uncover a novel chromatin-mediated regulatory mechanism in which NF-YCs, in association with ELF7, control the transcription of FLM to modulate flowering time.\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of imprinted genes in citrus endosperm and their contributions to seed development 柑橘胚乳印迹基因的综合分析及其对种子发育的贡献

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-06-25 DOI: 10.1111/tpj.70290

Jing-Jing He, Gang Hu, Ming-Yao Shen, Yan-Jie Fan, Xiao-Shu Shi, Xiao-Meng Wu, Wen-Wu Guo, Qiang-Ming Xia, Kai-Dong Xie

{"title":"Comprehensive analysis of imprinted genes in citrus endosperm and their contributions to seed development","authors":"Jing-Jing He, Gang Hu, Ming-Yao Shen, Yan-Jie Fan, Xiao-Shu Shi, Xiao-Meng Wu, Wen-Wu Guo, Qiang-Ming Xia, Kai-Dong Xie","doi":"10.1111/tpj.70290","DOIUrl":"https://doi.org/10.1111/tpj.70290","url":null,"abstract":"<div>\u0000 \u0000 Interploidy hybridization between diploid and tetraploid has been an important approach to develop triploid hybrids in horticultural plants including citrus for seedless breeding. However, dysregulation of imprinted genes can lead to the failure of endosperm cellularization, resulting in abortion of triploid embryos before seed maturity, thereby impeding the efficiency of triploid generation. The identification of imprinted genes is essential for comprehending the impact of imprinting on endosperm cellularization and mitigating embryo abortion in interploidy hybridization. Herein, a genome-wide search for imprinted genes in citrus was performed using RNA sequencing of the endosperm collected from two pairs of reciprocal crosses and totally 296 imprinted genes were identified. Among them, the paternally imprinted genes (PEGs) showed a higher inclination toward endosperm-specific expression compared to maternally imprinted genes (MEGs), with a few demonstrating consistent imprinting across three developmental stages of endosperm and displaying homology with counterparts found in other plant species. The analysis of transposable element (TE) enrichment and DNA methylation revealed a significant enrichment of mutator TEs with higher level of DNA methylation around maternal imprinted genes, highlighting their vital role for controlling the expression of MEGs. By overexpressing the endosperm-specific expressed PEGs in Arabidopsis, we observed that the transgenic lines of OE-CsPEG1 exhibited an increased frequency of seed abortion, which appeared to be correlated with delayed endosperm cellularization, resembling the observed phenotypes in 2x × 4x interploidy hybridizations. Our research provides evidence supporting the functional conservation of imprinted genes in plants, thereby identifying potential targets for genetic improvement in triploid breeding.\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Translational landscape provides insight into the molecular mechanism of heterosis in inter-subspecific hybrid rice 翻译景观为亚种间杂交水稻杂种优势的分子机制提供了新的视角

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-06-24 DOI: 10.1111/tpj.70297

Zengde Xi, Mengyao Wang, Fei Wang, Jianbo Wang

{"title":"Translational landscape provides insight into the molecular mechanism of heterosis in inter-subspecific hybrid rice","authors":"Zengde Xi, Mengyao Wang, Fei Wang, Jianbo Wang","doi":"10.1111/tpj.70297","DOIUrl":"https://doi.org/10.1111/tpj.70297","url":null,"abstract":"<div>\u0000 \u0000 Heterosis has been widely applied in crop breeding and has significantly improved grain yield worldwide. Many studies have attempted to elucidate heterosis from various perspectives; however, its genetic basis—especially at the translational level—remains elusive. In this study, we performed RNA-seq and ribosome profiling on the inter-subspecific hybrid rice ZY19 (Oryza sativa L. subsp. indica Kato × O. sativa L. subsp. japonica Kato) and its parental lines to examine genome-wide translational dynamics. Differential gene expression between the hybrid and its parents revealed a strong discordance between transcriptional and translational levels, and translational regulation appeared to buffer the transcriptional differences. Although additive and non-additive gene expression patterns shifted during translation, additive expression remained the predominant pattern at the translational level in the hybrid. Moreover, a high proportion of single-parent expression genes also exhibited additive expression. In the hybrid, allele-specific expression (ASE) was differentially regulated in transcription and translation. Notably, cis and trans-regulation tended to function independently in transcription, whereas they were more likely to act together in translation. Finally, we investigated the effects of various regulatory mechanisms and elements on translation and found that genes with more alternative splicing (AS) events had a lower translational efficiency (TE) than genes with fewer AS events. In addition, translation was repressed by the upstream open reading frames (uORFs), downstream open reading frames (dORFs), N6-methyladenosines (m6As) and microRNAs (miRNAs). Overall, our study provides new insights into the molecular mechanisms of heterosis in inter-subspecific hybrid rice.\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confocal imaging of the cell cycle and cytokinin signaling during gynoecium development in Arabidopsis 拟南芥雌蕊发育过程中细胞周期和细胞分裂素信号的共聚焦成像

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-06-24 DOI: 10.1111/tpj.70299

Juan Ramos-Pulido, Stefan de Folter

{"title":"Confocal imaging of the cell cycle and cytokinin signaling during gynoecium development in Arabidopsis","authors":"Juan Ramos-Pulido, Stefan de Folter","doi":"10.1111/tpj.70299","DOIUrl":"https://doi.org/10.1111/tpj.70299","url":null,"abstract":"In angiosperms, the gynoecium is the female reproductive structure, whose function is to contain the ovules and promote their fertilization. In Arabidopsis, the gynoecium develops from a small primordium with meristematic properties to a complex structure with internal and external specialized tissues. Due to its anatomical and developmental complexity, it is challenging to study in vivo the developmental processes of the gynoecium. Although efforts have been made, there are still unanswered questions, especially those related to cell proliferation of internal tissues. Here, we use confocal imaging to study the cell cycle, the proliferation of internal tissues, and the relationship with cytokinin signaling. We observed that early gynoecium proliferation is complex and with a long cell cycle dynamics. Moreover, we observed a relationship between the region of highest proliferation and maximum cytokinin signaling, a rise in proliferation attributed to BAP treatment, ectopic tissue formation with cytokinin signaling, and alterations in the gynoecium primordium in mutant lines. Together, these results provide insights into cell cycle dynamics, meristematic maintenance, and medial tissue development in the Arabidopsis gynoecium.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70299","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dsRNA-binding protein OsDRB1.4 is phosphorylated by OsMPK5 and negatively regulates rice defense against Magnaporthe oryzae dsrna结合蛋白OsDRB1.4被OsMPK5磷酸化，负向调控水稻对稻瘟病菌的防御

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-06-24 DOI: 10.1111/tpj.70285

Yuedan Chen, Shuai Yin, Yinong Yang, Kabin Xie

{"title":"The dsRNA-binding protein OsDRB1.4 is phosphorylated by OsMPK5 and negatively regulates rice defense against Magnaporthe oryzae","authors":"Yuedan Chen, Shuai Yin, Yinong Yang, Kabin Xie","doi":"10.1111/tpj.70285","DOIUrl":"https://doi.org/10.1111/tpj.70285","url":null,"abstract":"<div>\u0000 \u0000 Plant mitogen-activated protein kinases (MAPKs) are pivotal regulators of the innate immune signaling pathway and phosphorylate an array of transcription factors to reprogram the expression of disease-responsive genes. However, how MAPKs reprogram plant gene expression at the posttranscriptional level is poorly understood. Here, we show that double-stranded RNA-binding protein 1.4 (OsDRB1.4) is a substrate of OsMPK5 in rice. OsDRB1.4 belongs to a monocot-specific subgroup of the DRB family and contains a noncanonical MAPK interaction motif in its C terminus. OsMPK5 mainly phosphorylates the 216th serine of OsDRB1.4 in vivo. Silencing and knockout of OsDRB1.4 increased rice defense against Magnaporthe oryzae and increased the expression of 34 genes, including PR5 and three R-GENE ANALOG genes, whereas OsDRB1.4-overexpressing rice was more susceptible to blast disease. In contrast, overexpressing OsDRB1.4 with the phosphorylation sites mutated in rice did not affect disease susceptibility, suggesting that these phosphorylation sites are critical for OsDRB1.4 functionality. A total of 130 transcript units and 171 annotated repetitive sequence transcripts were identified as the RNA partners of OsDRB1.4 through targeted RNA editing. RNA electrophoretic mobility shift assays validated that OsDRB1.4 directly bound the stem-loop dsRNAs of microRNA precursors and the 3′-UTRs of protein-coding mRNAs. Further analysis indicated that OsDRB1.4 positively regulated the expression of mature osa-miR7695 and osa-miR2871. More importantly, OsDRB1.4 bound stem-loop dsRNA in the 3′-UTR of Pi-ta RESISTANCE (Ptr) and negatively affected protein expression. Together, our data reveal that the OsMPK5-OsDRB1.4 module negatively regulates rice defense against M. oryzae through the posttranscriptional suppression of defense-related genes.\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis TNL immune receptor BNT1 localizes to the plastid envelope and is required for the flg22-induced resistance against Pseudomonas 拟南芥TNL免疫受体BNT1定位于质体包膜，是flg22诱导的假单胞菌抗性所必需的

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-06-24 DOI: 10.1111/tpj.70295

Micaela Y. Peppino Margutti, Ana P. Cislaghi, Ariel Herrera-Vásquez, Julieta R. Palomeque, Francisco R. Bellino Tabera, María E. Alvarez, Francisca Blanco-Herrera, Nicolás M. Cecchini

{"title":"The Arabidopsis TNL immune receptor BNT1 localizes to the plastid envelope and is required for the flg22-induced resistance against Pseudomonas","authors":"Micaela Y. Peppino Margutti, Ana P. Cislaghi, Ariel Herrera-Vásquez, Julieta R. Palomeque, Francisco R. Bellino Tabera, María E. Alvarez, Francisca Blanco-Herrera, Nicolás M. Cecchini","doi":"10.1111/tpj.70295","DOIUrl":"https://doi.org/10.1111/tpj.70295","url":null,"abstract":"<div>\u0000 \u0000 Precise localization and trafficking of plant immune receptors are critical for their function. We identify the TNL-class nucleotide-binding leucine-rich repeat receptor (NLR) BURNOUT1 (BNT1) from Arabidopsis thaliana as localized to plastids, key organelles for plant immunity. Alternative transcription start site usage generates two isoforms of BNT1: BNT1.2, which is targeted to the plastid envelope via an N-terminal signal-anchored mechanism, and BNT1.1, which resides in the cytoplasm. Moreover, BNT1.2 is predominantly expressed in epidermal cells, where it localizes to the so-called sensory plastids. Functional analysis revealed that bnt1 mutants exhibit compromised PAMP-triggered immunity (PTI) responses, including impaired callose deposition and reduced flg22-induced resistance to Pseudomonas syringae pv. tomato, while flg22-induced apoplastic reactive oxygen species production remains unaffected. Notably, only the plastid-localized BNT1.2 isoform is required for these PTI responses. Our findings reveal a role for NLRs in regulating PTI responses from plastids and highlight these organelles as key hubs for signal(s) integration during plant–pathogen interactions.\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Algal origins of core land plant stress response subnetworks 陆生植物核心逆境响应子网络的藻类起源

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-06-23 DOI: 10.1111/tpj.70291

Armin Dadras, Pauline Duminil, Sophie de Vries, Iker Irisarri, Ivo Feussner, Jan de Vries

引用次数: 0

From data to discovery: leveraging big data in plant natural products biosynthesis research 从数据到发现：利用大数据进行植物天然产物生物合成研究

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-06-22 DOI: 10.1111/tpj.70288

Matthew McConnachie, Tuan-Anh Minh Nguyen, Truc Kim, Trinh-Don Nguyen, Thu-Thuy T. Dang

{"title":"From data to discovery: leveraging big data in plant natural products biosynthesis research","authors":"Matthew McConnachie, Tuan-Anh Minh Nguyen, Truc Kim, Trinh-Don Nguyen, Thu-Thuy T. Dang","doi":"10.1111/tpj.70288","DOIUrl":"https://doi.org/10.1111/tpj.70288","url":null,"abstract":"Plant natural products or specialized metabolites play a vital role in drug discovery and development, with many clinically important derivatives such as the anticancer drugs topotecan (derived from the natural alkaloid camptothecin) and etoposide (derived from the natural polyphenol podophyllotoxin). Remarkable advances in understanding plant natural product metabolism have been achieved at an unprecedented pace over the past 15 years. The integration of high-throughput technologies in genomics, transcriptomics, and metabolomics has generated vast datasets that provide a more comprehensive understanding of plant metabolism. Additionally, advances in computational tools, machine learning, and data analytics have played a crucial role in processing and interpreting the massive amounts of newly available data, enabling researchers to uncover intricate regulatory networks and identify key components of biosynthetic pathways. This review navigates the evolving landscape of plant biosynthetic pathway elucidation accelerated by innovative multidisciplinary strategies that capitalize on big data. We highlight recent advances in plant-specialized biosynthesis that illustrate how big data are increasingly leveraged to unravel the complexities of plant metabolism.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0