The Plant Journal最新文献

{"title":"Indel mutation in transcription factor PabHLH2 regulates amygdalin accumulation and kernel bitterness in apricot","authors":"Meiling Zhang,&nbsp;Fengchao Jiang,&nbsp;Li Yang,&nbsp;Wenjian Yu,&nbsp;Juanjuan Ling,&nbsp;Yuzhu Wang,&nbsp;Junhuan Zhang,&nbsp;Haoyuan Sun","doi":"10.1111/tpj.70523","DOIUrl":"https://doi.org/10.1111/tpj.70523","url":null,"abstract":"<div>\u0000 \u0000 <p>Amygdalin, the phytochemical responsible for the characteristic bitterness of apricot (<i>Prunus armeniaca</i> L.) kernels, also exhibits significant bioactive properties and therapeutic potential. Genetic regulation of amygdalin content is therefore a key objective in apricot breeding programs aimed at quality improvement. In this study, we conducted quantitative trait loci (QTL) mapping to uncover the genetic basis of sweet–bitter differentiation in apricot kernels. We identified a 15-bp insertion/deletion (indel) polymorphism strongly related to kernel bitterness, with marker validation achieving 100% concordance across 601 apricot germplasm accessions. Notably, this polymorphic site is located within the helix–loop–helix (HLH) domain of the basic HLH (bHLH) transcription factor PabHLH2. Protein interaction analyses revealed that the 15-bp deletion variant impaired dimerization capacity, reducing transcriptional activation of downstream targets. Using yeast one-hybrid screening and dual-luciferase reporter assays, we identified <i>PaCYP71AN24</i> and <i>PaCYP79D16</i> as direct transcriptional targets of PabHLH2. Functional characterization further indicated that the PabHLH2a variant (harboring the 15-bp insertion) significantly enhanced the promoter activity of these cytochrome P450 genes compared with the deletion variant. Transient overexpression and silencing experiments in apricot kernels further confirmed that the 15-bp insertion positively regulates both <i>PaCYP71AN24</i>/<i>PaCYP79D16</i> expression and prunasin accumulation, the immediate biosynthetic precursor of amygdalin. Overall, these findings provide mechanistic insights into the allelic variation underlying kernel bitterness and delineate the molecular cascade of amygdalin biosynthesis. The identified molecular markers and functional characterization establish a basis for marker-assisted breeding of low-amygdalin apricot cultivars, supporting the dual-purpose utilization of kernels in food and pharmaceutical industries.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145272441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canonical tyrosine-based motifs are required for constitutive endocytosis and polarity of IRT1 and contribute to metal uptake","authors":"Julien Spielmann,&nbsp;Nathalie Leonhardt,&nbsp;Julie Neveu,&nbsp;Grégory Vert","doi":"10.1111/tpj.70524","DOIUrl":"10.1111/tpj.70524","url":null,"abstract":"<p>Endocytosis regulates the localization and abundance of plasma membrane proteins. Several endocytic mechanisms were shown to operate in plants. The plant metal transporter IRT1 was previously shown to undergo ubiquitin-mediated endocytosis and degradation upon excess of some of its metal substrates. However, the contribution of other endocytic mechanisms to IRT1 internalization and plant metal nutrition remains unclear. Here, we uncovered that the core machinery of clathrin-mediated endocytosis (CME) participates in constitutive IRT1 endocytosis and plant metal nutrition. IRT1 directly interacts with AP2M through cytosolic-exposed YxxΦ motifs. Alteration of IRT1 CME using point mutation in AP2 recognition motifs or <i>ap2m</i> knockout leads to defects in IRT1 endocytosis, secretion/recycling and polarity, irrespective of metal nutrition. Altered IRT1 subcellular localization is associated with impaired growth responses to non-iron metals. Altogether, our work highlights the importance of AP2 complex-mediated recognition of IRT1 YxxΦ motifs for the constitutive trafficking of IRT1 and its role in plant metal uptake.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12515080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of DCL4 activity by maternally supplied flavonoid aglycons induces a bicolor pattern in the saddle soybean seed coat","authors":"Riho Yamanashi,&nbsp;Kazunori Kuriyama,&nbsp;Keita Sawai,&nbsp;Hiroshi Tsugawa,&nbsp;Hisashi Koiwa,&nbsp;Hiromitsu Moriyama,&nbsp;Toshiyuki Fukuhara","doi":"10.1111/tpj.70522","DOIUrl":"10.1111/tpj.70522","url":null,"abstract":"<p>Domesticated soybean (<i>Glycine max</i>) varieties can be divided into three major groups based on seed coat color: yellow (colorless), bicolored (saddle), and black. In yellow cultivars, the expression of the gene encoding chalcone synthase, essential for flavonoid biosynthesis, is inhibited by post-transcriptional gene silencing (PTGS). In saddle cultivars, PTGS is spatially inhibited in the central region around the hilum, which is black. However, the molecular mechanism of this region-specific inhibition of PTGS remains unclear. This study examined the relationship between Dicer-like 4 (DCL4), essential for PTGS, and flavonoid aglycons, which can inhibit DCL4. In the immature seed coat, DCL4 activity was specifically detected in the region that becomes colorless after seed maturation, but it was not detected in the region that becomes black after seed maturation, although its expression level had no difference. By contrast, phenolic compounds, including flavonoids, accumulated specifically in the region that becomes black. Especially, quercetin accumulated specifically in the central region of immature saddle seed coat and inhibited the dicing activity of DCL4. Furthermore, flavonoids highly accumulated in the funiculi of saddle and black but not yellow cultivars, and immature seeds cultured <i>in vitro</i> had reduced phenolic compounds in their seed coats. These results indicate that flavonoid aglycons including quercetin transported from the maternal tissues via the funiculus, accumulated in the central region of the immature seed coat, and induced bicolor pigmentation in the saddle seed coat by region-specific inhibition of DCL4 (PTGS).</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12515062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chloroplast 16S rRNA dimethyltransferase BrPFC1 is required for Brassica rapa development under chilling stress","authors":"Mengyang Liu,&nbsp;Xiangjie Su,&nbsp;Ziwei Xie,&nbsp;Qing Zhao,&nbsp;Xiaomeng Zhang,&nbsp;Yunran Zhang,&nbsp;Yin Lu,&nbsp;Wei Ma,&nbsp;Jianjun Zhao","doi":"10.1111/tpj.70516","DOIUrl":"10.1111/tpj.70516","url":null,"abstract":"<p>Chloroplast ribosomal RNA (Ch-rRNA) methylation is critical for plant development and response to low temperatures. Several Ch-rRNA methyltransferases and their catalytic modes, as well as biological relevance, have been reported in model plant species. However, Ch-rRNA methyltransferases and their functional significance remain poorly characterized in crops, including leafy vegetables such as Chinese cabbage. In this study, we screened an EMS-mutagenized Chinese cabbage population and identified a <i>yellow inner leaf</i> (<i>yif</i>) mutant. This mutant develops yellowing inner leaves with reduced chlorophyll accumulation and ultrastructure-impaired chloroplasts under low-temperature conditions. Genetic analysis revealed a premature termination mutation in <i>BrPFC1</i>, encoding the chloroplast-localized 16S rRNA dimethyltransferase. The BrPFC1 mutation (<i>yif</i>) disrupts the dimethylation of 16S rRNA. The cold-sensitive phenotype of the <i>yif</i> mutant can be explained by temperature-dependent defects in the maturation and assembly of chloroplast ribosomes at 4°C. Through integrated analysis of chloroplast and nuclear transcriptomes coupled with translational profiling at 25°C and 4°C, we established that low temperature preferentially upregulates transcripts encoding nuclear-derived ribosomal proteins, while defective 16S rRNA specifically compromises the translational efficiency of chloroplast-encoded photosynthetic complex and ribosomal protein at 4°C. These findings establish rRNA modification by BrPFC1 as a critical regulatory layer for optimizing chloroplast translational efficiency at 4°C, providing mechanistic insights into post-translational adaptation strategies in Chinese cabbage.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The MdGAMYB-MdHVA22g module confers drought tolerance by mediating γ-aminobutyric acid content and reactive oxygen species scavenging","authors":"Pengda Cheng,&nbsp;Xinyue Yang,&nbsp;Jingyu Zhang,&nbsp;Xiaoqian Xia,&nbsp;Yue Li,&nbsp;Jieqiang He,&nbsp;Dehui Zhang,&nbsp;Yutian Zhang,&nbsp;Fang Ma,&nbsp;Fengwang Ma,&nbsp;Chundong Niu,&nbsp;Qingmei Guan","doi":"10.1111/tpj.70503","DOIUrl":"10.1111/tpj.70503","url":null,"abstract":"<div>\u0000 \u0000 <p>HVA22 is an abscisic acid (ABA)- and stress-induced protein. However, how it is regulated and whether it plays a role under drought stress are largely unclear. In this study, we found that drought-inducible MdHVA22g plays a positive role under drought stress. Overexpression of <i>MdHVA22g</i> impairs endoplasmic reticulum (ER) morphology. Further analysis demonstrated that MdHVA22g enhances drought tolerance by upregulating <i>MdGAD1</i> and <i>MdGAD4</i> expression, thereby leading to increased GAD activity, γ-aminobutyric acid (GABA) accumulation, and enhanced reactive oxygen species (ROS) scavenging. In addition, we identified MdGAMYB as an upstream regulator of <i>MdHVA22g</i>. MdGAMYB, the drought-positive regulator, is able to directly bind to the promoter of <i>MdHVA22g</i> and activate its expression in response to drought, which results in increased GAD activity, GABA biosynthesis, and ROS detoxification. Therefore, the regulatory cascade of MdGAMYB-MdHVA22g enhances drought tolerance by facilitating ER stress-mediated GABA accumulation and subsequent ROS detoxification in apple. Collectively, our findings reveal a novel regulatory factor of MdHVA22g and elucidate the role of the MdGAMYB-MdHVA22g module in drought tolerance.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolites change reveal insights into the heartwood color formation mechanism of Dalbergia odorifera","authors":"Suhong Ren,&nbsp;Lirong Yan,&nbsp;Qiming Feng,&nbsp;Zhangjing Chen,&nbsp;Jinmei Xu,&nbsp;Rongjun Zhao","doi":"10.1111/tpj.70486","DOIUrl":"10.1111/tpj.70486","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Dalbergia odorifera</i> T. Chen, an indigenous species in Hainan Island, China, had high economic and cultural values. Its sapwood was light yellow, while heartwood was rich in colors of red, yellow, orange, and black. The colorful oily deposits were found in the vessels, fibers, and parenchyma cells of the heartwood. The contents of extractives, flavonoid, and anthocyanin in heartwood were found to be 11.83%, 34.88 mg/g, and 705.84 μg/g, 3.29 times, 12.37 times, and 9.51 times higher than those of sapwood, respectively. More extractives, flavonoids, and anthocyanins appeared in the transition zone in the heartwood area position with abundant metabolites. Among them, flavonoids accounted for 26.2%. The relative content of metabolites, such as anthocyanins, chalcone, flavone, and anthraquinone increased greatly in heartwood. The formation of the red–yellow–orange mixed color of <i>D. odorifera</i> heartwood was related to these metabolites.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In conversation with Dr. Beronda Montgomery","authors":"Luis De Luna Valdez","doi":"10.1111/tpj.70513","DOIUrl":"10.1111/tpj.70513","url":null,"abstract":"&lt;p&gt;@BerondaM&lt;/p&gt;&lt;p&gt;Professor Beronda Montgomery is Professor of Biology at Grinnell College, USA. Her work integrates plant biology with leadership, mentoring, and institutional change. Trained as a plant biologist, she has advanced the understanding of photosynthetic organisms and ecological principles to inform community-building in academic life. Her career exemplifies how disciplinary expertise can serve as a foundation for broader reflections on equity, growth, and the redefinition of success in science.&lt;/p&gt;&lt;p&gt;In this interview, Professor Montgomery offers insights that challenge traditional models of success and invite a more relational, inclusive, and sustainable vision for scientific practice. Professor Montgomery emphasizes that thriving – whether for plants or people – cannot be explained by narrow productivity metrics. Instead, she highlights the importance of environments that provide access to resources, foster authentic growth, and value diverse pathways to success. For her, mentoring is distinct from advising: rather than simply guiding individuals toward institutional milestones, mentoring requires intentional listening and recognition of the full humanity of mentees while leveraging one's resources to support their personal goals. Her vision extends to the institutional level, where she calls for a critical reassessment of entrenched practices and policies that limit inclusion.&lt;/p&gt;&lt;p&gt;&lt;b&gt;1. Your work bridges the worlds of science, mentorship, and personal growth. What first inspired you to explore these intersections?&lt;/b&gt;&lt;/p&gt;&lt;p&gt;Shortly after starting my own research group as an Assistant Professor, I realized that while I had been successfully mentored during my PhD and postdoctoral years, the mentoring I received from my two advisors was very different. I wanted to explore effective mentoring styles, and – as most scientists do when trying a new protocol – I turned to the literature. This led me to identify many peer-reviewed articles on mentoring in the sciences, most published in education and psychology. I began incorporating those insights into my own practices and into the communities of practice in which I was engaged.&lt;/p&gt;&lt;p&gt;&lt;b&gt;2. How has your training as a plant biologist shaped the way you approach leadership, mentorship, and community-building in science?&lt;/b&gt;&lt;/p&gt;&lt;p&gt;Because my work with plants and photosynthetic bacteria often centers on ecosystem-based interactions – including individual–environment and individual–individual relationships – I have carried this systems-based perspective into my efforts as a mentor and leader.&lt;/p&gt;&lt;p&gt;&lt;b&gt;3. You often speak of learning from plants. Can you share an early moment when you realized that your scientific observations could also inform your thinking about people?&lt;/b&gt;&lt;/p&gt;&lt;p&gt;One of the earliest moments I recall in a professional setting was during a discussion early in my time as an Assistant Professor. A steering committee for an interdepartmental graduate program was considering se","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70513","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T-DNA orientation, distance between two T-DNAs, and the transformation target cells significantly impact vector backbone integration and efficiency of generating marker-free transgenic plants in a co-transformation system","authors":"Mariam Al Nuaimi,&nbsp;Mohammed Rafi,&nbsp;Mohamed ElSiddig,&nbsp;Maitha Aldarmaki,&nbsp;Suja George,&nbsp;Khaled M.A. Amiri","doi":"10.1111/tpj.70510","DOIUrl":"10.1111/tpj.70510","url":null,"abstract":"<p>The development of marker-free transgenic plants is essential to address biosafety concerns and facilitate regulatory approval. Co-transformation strategies involving separate T-DNAs for the gene of interest and selectable marker gene offer a clean approach but are often hampered by linked integration and vector backbone incorporation. In this study, we designed and evaluated a series of double T-DNA vectors with varying intervening sequence lengths and orientations to determine their impact on co-transformation efficiency and integration patterns in different plant species. Our results showed that shorter spacer regions increased the likelihood of linked T-DNA integration, while an ~3 kb intervening region minimized this risk. Contrary to previous findings, inverse orientation of T-DNAs with respect to each other in the vector significantly increased the frequency of linked and closely spaced integrations compared to tandem arrangements. Co-transformation efficiency and integration outcomes varied across species and transformation methods, with Arabidopsis exhibiting higher rates of linked integration possibly due to germline transformation via floral dip, in contrast to somatic cell transformation in tobacco, lettuce, and tomato. Incorporation of a GFP reporter gene within the intervening region enabled easy identification of unlinked integration events in the T0 generation, reducing downstream screening efforts. Marker-free plants were successfully recovered in the T1 generation, confirming the effectiveness of this approach. These findings emphasize the importance of T-DNA design, orientation, and target cell type in optimizing co-transformation strategies for generating marker-free transgenic plants.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12503609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic allopolyploidy unveils hybridization-driven transcriptional reprogramming underlying thermal adaptation in Cucumis","authors":"Yun Pei,&nbsp;Xiaokun Zhao,&nbsp;Weixuan Du,&nbsp;Weiping Diao,&nbsp;Wanping Zhang,&nbsp;Biao Xiong,&nbsp;Guillaume P. Ramstein,&nbsp;Carl-Otto Ottosen,&nbsp;Chunyan Cheng,&nbsp;Qinzheng Zhao,&nbsp;Ji Li,&nbsp;Qunfeng Lou,&nbsp;Jinfeng Chen,&nbsp;Xiaqing Yu","doi":"10.1111/tpj.70507","DOIUrl":"10.1111/tpj.70507","url":null,"abstract":"<div>\u0000 \u0000 <p>Both heterosis (hybrid vigor) resulting from hybridization and genetic plasticity conferred by whole-genome duplication (WGD) are recognized as drivers of evolutionary success and ecological adaptation in plants. Allopolyploids, which combine both hybridization and WGD, are widespread in both natural and agricultural settings and often exhibit superior performance. However, the relative contributions of these two elements to the success of allopolyploids remain poorly understood. Here, we employed an experimentally reconstructed allotetraploid <i>Cucumis</i> species (<i>C</i>. × <i>hytivus</i>, 2n = 4<i>x</i> = 38) and its diploid interspecific hybrid progenitor (allodiploid, 2n = 2<i>x</i> = 19) to decouple and investigate the distinct and combined contributions of hybridization and whole-genome doubling to immediate genetic and phenotypic consequences of allopolyploid formation under environmental stress. Both <i>C</i>. × <i>hytivus</i> and the allodiploid exhibited superior heat tolerance compared with the parental species with significantly higher semi-lethal temperature and enhanced physiological acclimation capacity. While the allodiploid and allotetraploid retain transcriptomic features where differences persist (e.g., WGCNA modules), comparative analysis of the 15,680 homoeologous gene pairs in the allodiploid and allotetraploid under heat stress (45°C) versus control conditions (28°C) revealed conserved heat-responsive transcriptional plasticity, suggesting that enhanced thermotolerance in <i>C</i>. × <i>hytivus</i> is presented as consequences arising dominantly after interspecific hybridization. This study provides mechanistic insights into allopolyploid adaptation through experimental reconstruction of allopolyploid genomes, demonstrating that hybridization initiates key transcriptional and physiological advantages under stress, subsequent WGD stabilizes these adaptations and contributes to the full phenotypic realization. This work decouples the roles of interspecific hybridization and WGD and proposes a synthetic biology approach for developing climate-resilient crops.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jasmonate primes plant responses to extracellular ATP","authors":"Jeremy B. Jewell,&nbsp;Ashleigh S. Carlton,&nbsp;Jordan P. Tolley,&nbsp;Laura E. Bartley,&nbsp;Kiwamu Tanaka","doi":"10.1111/tpj.70514","DOIUrl":"10.1111/tpj.70514","url":null,"abstract":"<p>Extracellular ATP (eATP) signaling in <i>Arabidopsis thaliana</i> is mediated by the purinoceptor P2K1. Previous studies have clarified that the downstream transcriptional responses to eATP involve jasmonate (JA)-based signaling components such as the JA receptor (COI1) and JA-responsive bHLH transcription factors (MYCs). However, the specific contributions of JA itself on eATP signaling are unexplored. Here, we report that JA primes plant responses to eATP through P2K1. Our findings show that JA treatment significantly upregulates <i>P2K1</i> transcription, corroborating our observation that JA facilitates eATP-induced cytosolic calcium elevation and transcriptional reprogramming in a JA signaling-dependent manner. Additionally, we find that salicylic acid pre-treatment represses eATP-induced plant response. These results suggest that JA accumulation during biotic or abiotic stresses potentiates eATP signaling, enabling plants to better cope with subsequent stress events.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"124 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12498120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145230959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}