The Plant Journal最新文献

{"title":"LTD1 plays a key role in rice tillering regulation through cooperation with CycH1;1 and TFB2 subunits of the TFIIH complex","authors":"Xiaorong Yang,&nbsp;Chun Hu,&nbsp;Xiangyu Zhang,&nbsp;Xiaolan Wang,&nbsp;Longfei Chen,&nbsp;Hongshan Zhang,&nbsp;Xinxin Ma,&nbsp;Ke Liang,&nbsp;Congping Chen,&nbsp;Jia Guo,&nbsp;Chun Li,&nbsp;Bin Yang,&nbsp;Changhui Sun,&nbsp;Xiaojian Deng,&nbsp;Pingrong Wang","doi":"10.1111/tpj.70119","DOIUrl":"https://doi.org/10.1111/tpj.70119","url":null,"abstract":"<div>\u0000 \u0000 <p>Tillering contributes greatly to grain yield in rice (<i>Oryza sativa</i>). At present, many genes involved in rice tillering regulation have been cloned and characterized. However, the identification of more novel genes is still necessary to fully understand the molecular mechanisms regulating rice tillering. In this study, we isolated a <i>low</i>-<i>tillering and dwarf 1</i> (<i>ltd1</i>) mutant in <i>indica</i> rice. Map-based cloning and MutMap analysis showed that the candidate gene <i>LTD1</i> (<i>LOC_Os01g19760</i>) encodes a putative FAM91A1 protein with an unknown function in plants. <i>LTD1</i>-complementation and -RNAi confirmed that <i>LTD1</i> is responsible for the mutant phenotype of <i>ltd1</i>. The LTD1 protein is localized to the plasma membrane, endoplasmic reticulum, and multi-vesicular bodies. Furthermore, protein interaction and colocalization assays showed that LTD1 interacts with both the TFB2 subunit of the core subcomplex and the CycH1;1 subunit of the cyclin-dependent kinase-activating kinase (CAK) subcomplex of the TFIIH complex, and TFB2 also interacts with CycH1;1. qRT-PCR demonstrated that the expression levels of most genes related to the cell cycle are changed significantly in the <i>ltd1</i> tiller buds, and flow cytometry assays revealed that there are more polyploid nuclei in the <i>ltd1</i> leaves and roots, suggesting that LTD1 could be involved in cell cycle regulation. Taken together, our findings indicated that <i>LTD1</i> plays a key role in rice tillering regulation by involvement in the cell cycle through cooperation with CycH1;1 and TFB2 subunits of TFIIH. This work also sheds light on the biological function of FAM91A1 in regulating important agronomic traits of rice.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential methylation of a retrotransposon upstream of a MYB gene causes variegation of lettuce leaves, which is abolished by the presence of an (AT)5 repeat in the promoter","authors":"Rong Tao,&nbsp;Jiaojiao Ma,&nbsp;Jinlong Qian,&nbsp;Yali Liu,&nbsp;Weiyi Zhang,&nbsp;Dean Lavelle,&nbsp;Xin Wang,&nbsp;Wenhao Yan,&nbsp;Richard W. Michelmore,&nbsp;Jiongjiong Chen,&nbsp;Hanhui Kuang","doi":"10.1111/tpj.70123","DOIUrl":"https://doi.org/10.1111/tpj.70123","url":null,"abstract":"<div>\u0000 \u0000 <p>Variegation, a common phenomenon in plants, can be the result of several genetic, developmental, and physiological factors. Leaves of some lettuce cultivars exhibit dramatic red variegation; however, the genetic mechanisms underlying this variegation remain unknown. In this study, we cloned the causal gene for variegation on lettuce leaves and elucidated the underlying molecular mechanisms. Genetic analysis revealed that the polymorphism of variegated versus uniformly red leaves is caused by an “AT” repeat in the promoter of the <i>RLL2A</i> gene encoding a MYB transcription factor. Complementation tests demonstrated that the <i>RLL2A</i> allele (<i>RLL2A</i>^<i>V</i>) with (AT)_n repeat numbers other than five led to variegated leaves. <i>RLL2A</i>^<i>V</i> was expressed in the red spots but not in neighboring green regions. This expression pattern was in concert with a relatively low level of methylation in a retrotransposon inserted in −761 bp of the gene in the red spots compared to high methylation of the retrotransposon in the green region. The presence of (AT)₅ in the promoter region, however, stabilized the expression of <i>RLL2A</i>, resulting in uniformly red leaves. In summary, we identified a novel promoter mechanism controlling variegation through inconsistent levels of methylation and showed that the presence of a simple sequence repeat of specific size could stabilize gene expression.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anther-specific expression of MsMYB35 transcription factor in alfalfa (Medicago sativa L.) and its crucial role in pollen development","authors":"Huicai Cai,&nbsp;Shuhe Zhang,&nbsp;Yingzhe Wang,&nbsp;Zhenning Yang,&nbsp;Lin Zhang,&nbsp;Jiahao Zhang,&nbsp;Minmin Zhang,&nbsp;Bo Xu","doi":"10.1111/tpj.70126","DOIUrl":"https://doi.org/10.1111/tpj.70126","url":null,"abstract":"<div>\u0000 \u0000 <p>Alfalfa (<i>Medicago sativa</i> L.) is a high-quality forage crop and an essential resource for livestock. Understanding the molecular mechanisms underlying male sterility in alfalfa is pivotal for the development of superior forage varieties. Despite the critical role of anther development in plant reproduction, its molecular regulation—particularly the involvement of transcription factors in <i>M. sativa</i>—remains insufficiently explored. This study bridges this gap by isolating and characterizing an R2R3-MYB transcription factor, <i>MsMYB35</i>, and unveiling its regulatory role in anther development. Quantitative RT-PCR (qRT-PCR) revealed that <i>MsMYB35</i> is predominantly expressed during early anther development and is homologous to <i>AtMYB35</i>. <i>MsMYB35</i> was found to localize in both the cytoplasm and nucleus. DNA affinity purification sequencing (DAP-seq) identified 3647 target genes of <i>MsMYB35</i>, with enrichment analysis uncovering three recognition motifs. Integrated DAP-seq and RNA-seq analyses revealed that <i>MsMYB35</i> directly regulates two key anther development-related genes. Functional analyses showed that overexpression of <i>MsMYB35</i> promotes anther development, while silencing <i>MsMYB35</i> leads to defective anther sacs and wrinkled pollen grains. Proper <i>MsMYB35</i> expression ensures the formation of viable and fertile pollen grains, solidifying its role as a critical regulator of anther development. These findings provide a novel perspective on the molecular mechanisms regulating anther development in <i>M. sativa</i> and offer valuable insights for improving molecular breeding and hybrid seed production strategies. By advancing the fundamental understanding of transcriptional regulation in anther development, this study sets the stage for innovative approaches to alfalfa crop improvement.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multi-omics analyses provide new insights into genomic variation landscape and regulatory network candidate genes associated with walnut endocarp","authors":"Hengzhao Liu,&nbsp;Huijuan Zhou,&nbsp;Hang Ye,&nbsp;Mengdi Li,&nbsp;Jiayu Ma,&nbsp;Ruimin Xi,&nbsp;Xiaozhou He,&nbsp;Peng Zhao","doi":"10.1111/tpj.70113","DOIUrl":"https://doi.org/10.1111/tpj.70113","url":null,"abstract":"<div>\u0000 \u0000 <p>Persian walnut (<i>Juglans regia</i>) is an economically important nut oil tree; the fruit has a hard endocarp/shell to protect seeds, thus playing a key role in its evolution, and the shell thickness is an important trait for walnut breeding. However, the genomic landscape and the gene regulatory networks associated with walnut shell development remain to be systematically elucidated. Here, we report a high-quality genome assembly of the walnut cultivar ‘Xiangling’ and construct a graphic structure pan-genome of eight <i>Juglans</i> species to reveal the genetic variations at the genome level. We re-sequence 285 accessions to characterize the genomic variation landscape. Through genome-wide association studies (GWAS), we identified 19 loci associated with more than 268 loci that underwent selection during walnut domestication and improvement. Multi-omics analyses, including transcriptomics, metabolomics, DNA methylation, and spatial transcriptomics across eleven developmental stages, revealed several candidate genes related to secondary cell biosynthesis and lignin accumulation. This integrated multi-omics approach revealed several candidate genes associated with secondary cell biosynthesis and lignin accumulation, such as <i>UGP</i>, <i>MYB308, MYB83</i>, <i>NAC043</i>, <i>NAC073</i>, <i>CCoAOMT1</i>, <i>CCoAOMT7</i>, <i>CHS2</i>, <i>CESA7</i>, <i>LAC7</i>, <i>COBL4</i>, and <i>IRX12</i>. Overexpression of <i>JrUGP</i> and <i>JrMYB308</i> in <i>Arabidopsis thaliana</i> confirmed their roles in lignin biosynthesis and cell wall thickening. Consequently, our comprehensive multi-omics findings offer novel insights into walnut genetic variation and network regulation of endocarp development and shell thickness, which enable further genome-informed breeding strategies for walnut cultivar improvement.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autophagy positively regulates ethylene-induced colouration in citrus fruits","authors":"Ye Guo,&nbsp;Jinli Gong,&nbsp;Ran Hu,&nbsp;Meiyan Shi,&nbsp;Zhiru Bao,&nbsp;Saiyu Cao,&nbsp;Kaijie Zhu,&nbsp;Xiuxin Deng,&nbsp;Yunjiang Cheng,&nbsp;Pengwei Wang","doi":"10.1111/tpj.70114","DOIUrl":"https://doi.org/10.1111/tpj.70114","url":null,"abstract":"<div>\u0000 \u0000 <p>Autophagy is an evolutionarily conserved process in eukaryotes that regulates metabolic reprogramming and organelle recycling in response to various environmental signals and developmental cues. However, little is known about its regulatory mechanism during fruit colouration and ripening, which also undergo dramatic metabolic and cellular alterations. Here, we demonstrate that the autophagy pathway is activated during citrus fruit colouration, and the colour transition of citrus fruit is significantly delayed when autophagy is blocked. Furthermore, we revealed that ethylene, a plant hormone crucial for citrus fruit colouration, activates the autophagy pathway through the ethylene-responsive factor, CsERF061. Further analysis revealed that CsERF061 directly binds to the promoter of <i>CsATG8h</i> and activates its expression, thereby promoting autophagy and fruit colouration, suggesting autophagy is a key determinant of citrus fruit colouration in response to ethylene. These findings enhance our understanding of fruit colouration and offer a potential method to improve citrus fruit colour and quality for future applications.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthocyanins promote the abundance of endophytic lactic acid bacteria by reducing ROS in Medicago truncatula","authors":"Junjie Liu,&nbsp;Yuanyuan Huang,&nbsp;Huan Du,&nbsp;Jing Tian,&nbsp;Fan Zhu,&nbsp;Jianguo Zhang,&nbsp;Qing Zhang,&nbsp;Xiaolin Wang,&nbsp;Liangfa Ge","doi":"10.1111/tpj.70127","DOIUrl":"https://doi.org/10.1111/tpj.70127","url":null,"abstract":"<div>\u0000 \u0000 <p>The microbial community residing on the phyllosphere is influenced by many factors, including the host plant's genotype as well as its secondary metabolites. Anthocyanins are a group of flavonoids renowned for their antioxidative properties and are widely distributed across plant tissues. However, the potential impact of anthocyanins on plant-associated microbial communities remains unknown. In the model legume <i>Medicago truncatula</i>, we isolated a mutant named <i>purple leaves</i> (<i>pl</i>) that produces purple leaves at a young stage due to over-accumulated anthocyanins. Through sequencing 16S rRNA amplicons of phyllosphere microbes in the <i>pl</i> mutant, we show that anthocyanins significantly enhance the abundance of endophytic lactic acid bacteria within plant leaves. Further <i>in vitro</i> study revealed that anthocyanins derived from <i>pl</i> can significantly promote the growth of lactic acid bacteria under anaerobic conditions. The accumulated anthocyanins in <i>pl</i> leaves reduced reactive oxygen species (ROS), thereby creating a favorable environment for the growth of facultative anaerobic lactic acid bacteria and resultantly increasing the abundance of phyllosphere lactic acid bacteria. Our findings elucidate the role of anthocyanins in modulating the community structure of phyllosphere microbiota in <i>M. truncatula</i> and provide new insights into the relationship between plant secondary metabolites and phyllosphere microbiota.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolutionary trajectories and gene regulatory roles of nuclear-integrated plastid DNA: clues for enhancing environmental adaptation in Caryophyllales","authors":"Yi Yang,&nbsp;Xuan Liu,&nbsp;Binfang Fan,&nbsp;Yiran Wang,&nbsp;Shuaijie Wei,&nbsp;Ning Chen,&nbsp;Yulan Zhang,&nbsp;Shufen Li,&nbsp;Wujun Gao","doi":"10.1111/tpj.70131","DOIUrl":"https://doi.org/10.1111/tpj.70131","url":null,"abstract":"<div>\u0000 \u0000 <p>Environmental stimuli can induce the transfer of chloroplast DNA to the nuclear genome, resulting in nuclear-integrated plastid DNAs (NUPTs). However, their role in plant adaptability remains unclear. Species within the Caryophyllales order, known for their adaptation to extreme environments, provide an ideal model for studying the evolutionary dynamics and functions of NUPTs. In this study, we analyzed NUPTs in 24 Caryophyllales species to investigate their evolution and regulatory roles in gene expression, particularly in response to environmental stimuli. We found significant interspecies variation in NUPT abundance, ranging from 566 insertions in <i>Amaranthus cruentus</i> to 3585 in <i>Beta vulgaris</i>, with sizes spanning from 100 bp to over 100 kb. Approximately 62% of NUPTs were inserted within the last 20 million years, while some species exhibit insertion peaks dating back 49 million years. NUPT presence/absence polymorphisms in six related species suggest that NUPT insertions and deletions are dynamic processes influenced by phylogeny. NUPTs predominantly integrate into intergenic regions but also insert into genes and promoters, with certain regions acting as hotspots. Notably, NUPTs introduce numerous environmental-responsive <i>cis</i>-acting elements in promoter regions. Genes with NUPT insertions in their promoters are significantly enriched for functions related to environmental response. Further luciferase assays in <i>Spinacia oleracea</i> demonstrated that NUPT insertions can regulate the expression of genes related to environmental responses, indicating their potential role in adaptive evolution. Overall, our study provides insights into NUPT evolution and their influence on gene function and plant adaptability to environmental stimuli.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of xyloglucan metabolism mutants highlights the prominent role of xylose cleavage in seed dormancy","authors":"Hiromi Suzuki,&nbsp;Parisa Savane,&nbsp;Lucile Marion-Poll,&nbsp;Julien Sechet,&nbsp;Anne Frey,&nbsp;Adeline Berger,&nbsp;Katia Belcram,&nbsp;Nero Borrega,&nbsp;Mitsunori Seo,&nbsp;Aline Voxeur,&nbsp;Grégory Mouille,&nbsp;Annie Marion-Poll","doi":"10.1111/tpj.70063","DOIUrl":"https://doi.org/10.1111/tpj.70063","url":null,"abstract":"<p>Seed dormancy is an adaptive trait that delays germination until environmental conditions become favorable for seedling survival and growth. Germination has been shown to depend on the mechanical resistance strength of the covering layers (testa and endosperm) that counteracts the growth force of the embryo. Cell wall remodeling is essential in the regulation of germination processes. In <i>Arabidopsis thaliana</i>, the side chain trimming of xyloglucans (XyG), the major hemicellulose in cell walls, by the apoplastic XYLOSIDASE1 (XYL1), has been previously shown to regulate XyG side chain length and seed dormancy. To investigate to what extent side chain complexity impacts on cell wall mechanical properties and regulates seed germination, <i>xyl1</i> mutations were combined here with mutations in the two other glycosidases, the fucosidase AXY8 and the beta-galactosidase BGAL10. Analysis of germination phenotypes in <i>axy8 bgal10 xyl1</i> and in several XyG biosynthesis mutants did not show any link between dormancy depth and side chain length. The very specific effect of <i>xyl1</i> on seed dormancy in single and multiple mutants was clearly correlated with alterations in XyG intracellular localization, together with release and oxidation of free oligosaccharides (XGO). Accumulation of oxidized XGO could negatively impact cell wall remodeling by impairing remobilization and polarized secretion in cell walls, thus reducing growth anisotropy in elongating organs and modifying mechanical characteristics in seed tissues.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cut above: the critical roles of DICER-LIKE genes in Marchantia development","authors":"Martin Balcerowicz","doi":"10.1111/tpj.70121","DOIUrl":"https://doi.org/10.1111/tpj.70121","url":null,"abstract":"&lt;p&gt;Small RNAs (sRNAs), such as microRNAs (miRNAs) and small-interfering RNAs (siRNAs), play important roles in plant development, abiotic stress responses and immunity. These RNAs are generated by DICER-LIKE (DCL) ribonucleases, which cleave long RNA precursor molecules into 21–24 nucleotide double-stranded RNA fragments. In the case of miRNAs and siRNAs, these fragments are subsequently incorporated into RNA-induced silencing complexes (RISCs) and convey target specificity, resulting in transcriptional gene silencing or post-transcriptional repression through mRNA degradation or translational inhibition.&lt;/p&gt;&lt;p&gt;A single &lt;i&gt;DCL&lt;/i&gt; gene is present in green algae, whereas the gene family is diversified in streptophytes (Bélanger et al., &lt;span&gt;2023&lt;/span&gt;). DCL1, present in streptophyte algae and all land plant lineages, produces mature miRNAs from pri-miRNA precursors. DCL2, DCL3 and DCL4, responsible for generating various types of siRNAs, emerged in bryophytes (DCL3/4) and vascular plants (DCL2 in addition to DCL3/DCL4), respectively. Additionally, DCL5 represents a monocot-specific DCL that produces reproductive-phased siRNAs in anthers (Bélanger et al., &lt;span&gt;2023&lt;/span&gt;; Liu et al., &lt;span&gt;2009&lt;/span&gt;). While DCLs have been extensively studied in the model dicot &lt;i&gt;Arabidopsis thaliana&lt;/i&gt; (Arabidopsis), their roles in early diverging plant lineages remain largely unexplored.&lt;/p&gt;&lt;p&gt;Wolfgang Frank has a particular interest in bryophyte sRNAs; as some of the first plants to colonise terrestrial habitats, bryophytes had to develop specific molecular mechanisms to thrive on land. The diversification of sRNAs likely contributed to these adaptive processes. PhD student Erika Csicsely and post-doctoral researcher Oguz Top joined Frank's research group to investigate the molecular adaptations underlying the process of terrestrialisation. Top conducts comparative studies on the moss &lt;i&gt;Physcomitrium patens&lt;/i&gt; and the liverwort &lt;i&gt;Marchantia polymorpha&lt;/i&gt; (Marchantia) to uncover regulatory mechanisms that might have facilitated land plant adaptation. For the highlighted publication, he collaborated with Csicsely to investigate the role of &lt;i&gt;DCL&lt;/i&gt; genes in Marchantia.&lt;/p&gt;&lt;p&gt;Like other bryophytes, Marchantia possesses four &lt;i&gt;DCL&lt;/i&gt; genes (Bélanger et al., &lt;span&gt;2023&lt;/span&gt;), and Csicsely et al. confirmed that these belong to the &lt;i&gt;DCL1&lt;/i&gt;, &lt;i&gt;DCL3&lt;/i&gt; and &lt;i&gt;DCL4&lt;/i&gt; subclades, with two &lt;i&gt;DCL1&lt;/i&gt; sequences (Mp&lt;i&gt;DCL1a&lt;/i&gt;, Mp&lt;i&gt;DCL1b&lt;/i&gt;). The active site of DCL proteins is formed by a PAZ (Piwi/Argonaute/Zwille) domain, two ribonuclease III (RNase III) domains and a double-stranded RNA-binding site, and many DCLs harbour additional domains such as Helicase C, Dicer-dimer and restriction enzyme subunit III domains (Liu et al., &lt;span&gt;2009&lt;/span&gt;). Most of these domains are present in MpDCL1a, MpDCL3 and MpDCL4, but MpDCL1b only contains a PAZ and two RNase III domains. It thus appears that MpDCL1a is the canonical homologue of seed plant DCL1, wh","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BpMAPK3-mediated BpWRKY53 phosphorylation enhances Betula platyphylla drought stress tolerance by increasing flavonoid content","authors":"Wenfang Dong,&nbsp;Qingjun Xie,&nbsp;Jiaxin Li,&nbsp;Zhongyuan Liu,&nbsp;Zhibo Wang,&nbsp;Chao Wang,&nbsp;Caiqiu Gao","doi":"10.1111/tpj.70089","DOIUrl":"https://doi.org/10.1111/tpj.70089","url":null,"abstract":"<div>\u0000 \u0000 <p>The increasing intensity, frequency, and duration of drought pose a threat to the survival of some tree species worldwide, directly damaging the carbon sequestration capacity of forest ecosystems. Understanding the response mechanisms of trees to drought stress is particularly important. In this study, a drought stress regulatory network in <i>Betula platyphylla</i> (birch) was established by observing the changes in the root transcriptome at different drought stress time points (0, 3, 6, 24, 48, and 72 h), and a potential drought-resistant WRKY53 transcription factor was identified. The overexpression of <i>BpWRKY53</i> (OE-BpWRKY53) in birch enhanced drought tolerance. Yeast one hybrid (Y1H), electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation-PCR (ChIP-PCR), and dual-luciferase (dual-LUC) assays confirmed that BpWRKY53 positively activated <i>BpCHS3</i> and <i>BpCHSy</i> expression by binding to the W-box in their promoter, consequently increasing the flavonoid content in birch. Exogenous application of flavonoids enhances the drought tolerance of birch. BpMAPK3-mediated phosphorylation of BpWRKY53 at Ser201 enhances its ability to regulate the expression of <i>BpCHS3</i> and <i>BpCHSy</i>. Collectively, these results provide insights into the mechanism underlying drought-induced flavonoid biosynthesis through the transcriptional regulation of <i>BpCHS3</i> and <i>BpCHSy</i> mediated by BpWRKY53.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}