The Plant Journal最新文献_第3页

Role of an endodermis-specific miR858b-MYB1L module in the regulation of Taxol biosynthesis in Taxus mairei

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-04-05 DOI: 10.1111/tpj.70135

Chunna Yu, Danjin Zhang, Lingxiao Zhang, Zijin Fang, Yibo Zhang, Wanting Lin, Ruoyun Ma, Mengyin Zheng, Enhui Bai, Chenjia Shen

{"title":"Role of an endodermis-specific miR858b-MYB1L module in the regulation of Taxol biosynthesis in Taxus mairei","authors":"Chunna Yu, Danjin Zhang, Lingxiao Zhang, Zijin Fang, Yibo Zhang, Wanting Lin, Ruoyun Ma, Mengyin Zheng, Enhui Bai, Chenjia Shen","doi":"10.1111/tpj.70135","DOIUrl":"https://doi.org/10.1111/tpj.70135","url":null,"abstract":"<div>\u0000 \u0000 Taxol, a chemotherapeutic agent widely used for treating various cancers, is extracted from the stems of Taxus mairei. However, current knowledge regarding the effects of stem tissue and age on Taxol accumulation is limited. We employed matrix-assisted laser desorption/ionization mass spectrometry to visualize taxoids in stem section sections of varying ages from T. mairei. Laser capture microdissection integrated with data-dependent acquisition–MS/MS analysis identified that several Taxol biosynthesis pathway-related enzymes were predominantly produced in the endodermis, elucidating the molecular mechanisms underlying endodermis-specific Taxol accumulation. We identified an endodermis-specific MYB1-like (MYB1L) protein and proposed a potential function for the miR858-MYB1L module in regulating secondary metabolic pathways. DNA affinity purification sequencing analysis produced 92 506 target peaks for MYB1L. Motif enrichment analysis identified several de novo motifs, providing new insights into MYB recognition sites. Four target peaks of MYB1L were identified within the promoter sequences of Taxol synthesis genes, including TBT, DBTNBT, T13OH, and BAPT, and were confirmed using electrophoretic mobility shift assays. Dual-luciferase assays showed that MYB1L significantly activated the expression of TBT and BAPT. Our data indicate that the miR858b-MYB1L module plays a crucial role in the transcriptional regulation of Taxol biosynthesis by up-regulating the expression of TBT and BAPT genes in the endodermis.\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome editing in the green alga Chlamydomonas: past, present practice and future prospects

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-04-05 DOI: 10.1111/tpj.70140

Adrian P. Nievergelt

{"title":"Genome editing in the green alga Chlamydomonas: past, present practice and future prospects","authors":"Adrian P. Nievergelt","doi":"10.1111/tpj.70140","DOIUrl":"https://doi.org/10.1111/tpj.70140","url":null,"abstract":"The green alga Chlamydomonas is an important and versatile model organism for research topics ranging from photosynthesis and metabolism, cilia, and basal bodies to cellular communication and the cellular cycle and is of significant interest for green bioengineering processes. The genome in this unicellular green alga is contained in 17 haploid chromosomes and codes for 16 883 protein coding genes. Functional genomics, as well as biotechnological applications, rely on the ability to remove, add, and change these genes in a controlled and efficient manner. In this review, the history of gene editing in Chlamydomonas is put in the context of the wider developments in genetics to demonstrate how many of the key developments to engineer these algae follow the global trends and the availability of technology. Building on this background, an overview of the state of the art in Chlamydomonas engineering is given, focusing primarily on the practical aspects while giving examples of recent applications. Commonly encountered Chlamydomonas-specific challenges, recent developments, and community resources are presented, and finally, a comprehensive discussion on the emergence and evolution of CRISPR/Cas-based precision gene editing is given. An outline of possible future paths for gene editing based on current global trends in genetic engineering and tools for gene editing is presented.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of plant glycolysis and the tricarboxylic acid cycle by posttranslational modifications

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-04-04 DOI: 10.1111/tpj.70142

Ke Zheng, Maria del Pilar Martinez, Maroua Bouzid, Manuel Balparda, Markus Schwarzländer, Veronica G. Maurino

{"title":"Regulation of plant glycolysis and the tricarboxylic acid cycle by posttranslational modifications","authors":"Ke Zheng, Maria del Pilar Martinez, Maroua Bouzid, Manuel Balparda, Markus Schwarzländer, Veronica G. Maurino","doi":"10.1111/tpj.70142","DOIUrl":"https://doi.org/10.1111/tpj.70142","url":null,"abstract":"Plant glycolysis and the tricarboxylic acid (TCA) cycle are key pathways of central carbon metabolism. They facilitate energy transformation, provide redox balance, and supply the building blocks for biosynthetic processes that underpin plant survival, growth, and productivity. Yet, rather than acting as static pathways, the fluxes that are mediated by the enzymes involved form a branched network. Flux modes can change flexibly to match cellular demands and environmental fluctuations. Several of the enzymes involved in glycolysis and the TCA cycle have been identified as targets of posttranslational modifications (PTMs). PTMs can act as regulators to facilitate changes in flux by rapidly and reversibly altering enzyme organization and function. Consequently, PTMs enable plants to rapidly adjust their metabolic flux landscape, match energy and precursor provision with the changeable needs, and enhance overall metabolic flexibility. Here, we review the impact of different PTMs on glycolytic and TCA cycle enzymes, focusing on modifications that induce functional changes rather than the mere occurrence of PTMs at specific sites. By synthesizing recent findings, we provide a foundation for a system-level understanding of how PTMs choreograph the remarkable flexibility of plant central carbon metabolism.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The homeostasis of β-alanine is key for Arabidopsis reproductive growth and development

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-04-03 DOI: 10.1111/tpj.70134

Si Wu, Youjun Zhang, Urszula Luzarowska, Lei Yang, Mohamed A. Salem, Venkatesh P. Thirumalaikumar, Nir Sade, Vadim E. Galperin, Alisdair Fernie, Arun Sampathkumar, Shimon Bershtein, Corina M. Fusari, Yariv Brotman

{"title":"The homeostasis of β-alanine is key for Arabidopsis reproductive growth and development","authors":"Si Wu, Youjun Zhang, Urszula Luzarowska, Lei Yang, Mohamed A. Salem, Venkatesh P. Thirumalaikumar, Nir Sade, Vadim E. Galperin, Alisdair Fernie, Arun Sampathkumar, Shimon Bershtein, Corina M. Fusari, Yariv Brotman","doi":"10.1111/tpj.70134","DOIUrl":"https://doi.org/10.1111/tpj.70134","url":null,"abstract":"β-Alanine, an abundant non-proteinogenic amino acid, acts as a precursor for coenzyme A and plays a role in various stress responses. However, a comprehensive understanding of its metabolism in plants remains incomplete. Previous metabolic genome-wide association studies (mGWAS) identified ALANINE:GLYOXYLATE AMINOTRANSFERASE2 (AGT2, AT4G39660) linked to β-alanine levels in Arabidopsis under normal conditions. In this study, we aimed to deepen our insights into β-alanine regulation by conducting mGWAS under two contrasting environmental conditions: control (12 h photoperiod, 21°C, 150 μmol m−2 sec−1) and stress (harvested after 1820 min at 32°C and darkness). We identified two highly significant quantitative trait loci (QTL) for β-alanine, including the AGT2 locus associated in both environments and ALDEHYDE DEHYDROGENASE6B2 (ALDH6B2, AT2G14170) associated only under stress conditions. A coexpression-correlation network revealed that the regulatory pathway involving β-alanine levels, AGT2, and ALDH6B2 connects the branched chained amino acid (BCAA) degradation through the propionate pathway. Metabolic profiles of AGT2 overexpression (OE) and knock-out (KO) lines (agt2) across various organs and developmental stages established the critical role of AGT2 in β-alanine metabolism. This work underscores the importance of β-alanine homeostasis for proper growth and development in Arabidopsis.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The sucrose transporter TaSWEET11 is critical for grain filling and yield potential in wheat (Triticum aestivum L.)

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-04-03 DOI: 10.1111/tpj.70133

Mingming Wang, Jia Geng, Zhe Zhang, Wenxi Wang, Tian Ma, Pei Ni, Zihan Zhang, Xuanshuang Li, Jiewen Xing, Qixin Sun, Yufeng Zhang, Zhongfu Ni

{"title":"The sucrose transporter TaSWEET11 is critical for grain filling and yield potential in wheat (Triticum aestivum L.)","authors":"Mingming Wang, Jia Geng, Zhe Zhang, Wenxi Wang, Tian Ma, Pei Ni, Zihan Zhang, Xuanshuang Li, Jiewen Xing, Qixin Sun, Yufeng Zhang, Zhongfu Ni","doi":"10.1111/tpj.70133","DOIUrl":"https://doi.org/10.1111/tpj.70133","url":null,"abstract":"<div>\u0000 \u0000 Grain filling, a crucial process that determines grain weight, is regulated by the efficiency of sugar transport to the caryopsis. However, the regulation of sugar transport during this process in wheat remains largely unknown. In this study, we conducted genetic and transcriptomic analyses to investigate the role of TaSWEET11 in grain filling and its contribution to grain weight. TaSWEET11 encodes a membrane-localized protein and is primarily expressed in developing grains, specifically in the vascular bundle and nucellar projection. Knocking out TaSWEET11 disrupted starch synthesis in developing grains, resulting in shrunken and empty-pericarp grains. Further investigation revealed that TaSWEET11 is involved in sucrose transport, as knockout lines exhibited significantly reduced sucrose content. Transcriptomic analysis showed significant downregulation of genes related to starch synthesis and sucrose metabolism in knockout lines, shedding light on the mechanism behind grain shrinkage. Notably, overexpressing TaSWEET11 had a positive impact on effective tiller number, spike length, grain number per spike, and ultimately grain yield in CB037. In addition, TaSWEET11, as a key factor for grain filling, underwent strong selection during wheat domestication and breeding programs. Overall, these findings highlight the crucial role of TaSWEET11 in sucrose transport during grain filling and suggest its potential as a target for increasing wheat yield.\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutation of strigolactone biosynthetic gene DWARF 17 impairs the responses of rice tillering to N supply

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-04-01 DOI: 10.1111/tpj.70124

Xin Huang, Zhiming Kuang, Rui Zhou, Tiantian Liu, Li Tang, Zhipeng Gao, Tao Liu, Xiaorong Fan, Wei Xuan, Le Luo, Guohua Xu

{"title":"Mutation of strigolactone biosynthetic gene DWARF 17 impairs the responses of rice tillering to N supply","authors":"Xin Huang, Zhiming Kuang, Rui Zhou, Tiantian Liu, Li Tang, Zhipeng Gao, Tao Liu, Xiaorong Fan, Wei Xuan, Le Luo, Guohua Xu","doi":"10.1111/tpj.70124","DOIUrl":"https://doi.org/10.1111/tpj.70124","url":null,"abstract":"<div>\u0000 \u0000 Tiller number is one important parameter for rice yield and is influenced by both strigolactone (SL) and nitrogen (N). However, how SL and N interact to regulate the tiller outgrowth in rice is unclear. In this study, we isolated a multi-tillering mutant, tin, from an ethyl methanesulfonate (EMS)-mutagenized population of Wuyunjing 7, a japonica cultivar. The tin mutant exhibited low sensitivity to varying N concentrations during the tiller development. Through bulk segregation analysis (BSA), we identified a missense mutation located in the exon of DWARF 17 (D17), a key gene involved in SL biosynthesis. Complementation experiments confirmed that D17 is responsible for the tin tiller phenotype, and exogenous application of the SL analogue GR24 restored the tiller response of tin to N. Transcriptome analysis further revealed that D17 and SL regulate the tiller response to N by modulating the expression of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes and ammonium transporter genes. These findings elucidate the mechanism by which SL and N coordinate to regulate rice tillering growth, providing valuable insights for optimizing rice plant architecture to enhance yield potential.\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Twisted Sister1: an agravitropic mutant of bread wheat (Triticum aestivum) with altered root and shoot architectures

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-03-31 DOI: 10.1111/tpj.70122

Deying Zeng, Jiayu Peng, Lan Zhang, Mathew J. Hayden, Tina M. Rathjen, Xiaoqing Li, Wenfang Jiang, Emmanuel Delhaize

{"title":"Twisted Sister1: an agravitropic mutant of bread wheat (Triticum aestivum) with altered root and shoot architectures","authors":"Deying Zeng, Jiayu Peng, Lan Zhang, Mathew J. Hayden, Tina M. Rathjen, Xiaoqing Li, Wenfang Jiang, Emmanuel Delhaize","doi":"10.1111/tpj.70122","DOIUrl":"https://doi.org/10.1111/tpj.70122","url":null,"abstract":"<div>\u0000 \u0000 We identified a mutant of hexaploid wheat (Triticum aestivum) with impaired responses to gravity. The mutant, named Twisted Sister1 (TS1), had agravitropic roots that were often twisted along with altered shoot phenotypes. Roots of TS1 were insensitive to externally applied auxin, with the genetics and physiology suggestive of a mutated AUX/IAA transcription factor gene. Hexaploid wheat possesses over 80 AUX/IAA genes, and sequence information did not identify an obvious candidate. Bulked segregant analysis of an F2 population mapped the mutation to chromosome 5A, and subsequent mapping located the mutation to a 41 Mbp region. RNA-seq identified the TraesCS5A03G0149800 gene encoding a TaAUX/IAA protein to be mutated in the highly conserved domain II motif. We confirmed TraesCS5A03G0149800 as underlying the mutant phenotype by generating transgenic Arabidopsis thaliana. Analysis of RNA-seq data suggested broad similarities between Arabidopsis and wheat for the role of AUX/IAA genes in gravity responses, although there were marked differences. Here we show that the sequenced wheat genome, along with previous knowledge of the physiology of gravity responses from other plant species, gene mapping, RNA-seq, and expression in Arabidopsis have enabled the cloning of a key wheat gene that defines plant architecture.\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A space for time. Exploring temporal regulation of plant development across spatial scales

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-03-31 DOI: 10.1111/tpj.70130

Yadhusankar Sasidharan, Vijayalakshmi Suryavanshi, Margot E. Smit

{"title":"A space for time. Exploring temporal regulation of plant development across spatial scales","authors":"Yadhusankar Sasidharan, Vijayalakshmi Suryavanshi, Margot E. Smit","doi":"10.1111/tpj.70130","DOIUrl":"https://doi.org/10.1111/tpj.70130","url":null,"abstract":"Plants continuously undergo change during their life cycle, experiencing dramatic phase transitions altering plant form, and regulating the assignment and progression of cell fates. The relative timing of developmental events is tightly controlled and involves integration of environmental, spatial, and relative age-related signals and actors. While plant phase transitions have been studied extensively and many of their regulators have been described, less is known about temporal regulation on a smaller, cell-level scale. Here, using examples from both plant and animal systems, we outline time-dependent changes. Looking at systemic scale changes, we discuss the timing of germination, juvenile-to-adult transition, flowering, and senescence, together with regeneration timing. Switching to temporal regulation on a cellular level, we discuss several instances from the animal field in which temporal control has been examined extensively at this scale. Then, we switch back to plants and summarize examples where plant cell-level changes are temporally regulated. As time cannot easily be separated from signaling derived from the environment and tissue context, we next discuss factors that have been implicated in controlling the timing of developmental events, reviewing temperature, photoperiod, nutrient availability, as well as tissue context and mechanical cues on the cellular scale. Afterwards, we provide an overview of mechanisms that have been shown or implicated in the temporal control of development, considering metabolism, division control, mobile signals, epigenetic regulation, and the action of transcription factors. Lastly, we look at remaining questions for the future study of developmental timing in plants and how recent technical advancement can enable these efforts.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cysteine protease RD19C suppresses plant immunity to Phytophthora by modulating copper chaperone ATX1 stability

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-03-31 DOI: 10.1111/tpj.70120

Jingwen Dong, Weiwei Li, Yang Yang, Song Liu, Yilin Li, Yuling Meng, Weixing Shan

引用次数: 0

Heritable virus-induced germline editing in tomato

IF 6.2 1区生物学

The Plant Journal Pub Date : 2025-03-31 DOI: 10.1111/tpj.70115

Youngbin Oh, Ugrappa Nagalakshmi, Douglas Dahlbeck, Naio Koehler, Myeong-Je Cho, Savithramma P. Dinesh-Kumar, Brian J. Staskawicz

{"title":"Heritable virus-induced germline editing in tomato","authors":"Youngbin Oh, Ugrappa Nagalakshmi, Douglas Dahlbeck, Naio Koehler, Myeong-Je Cho, Savithramma P. Dinesh-Kumar, Brian J. Staskawicz","doi":"10.1111/tpj.70115","DOIUrl":"https://doi.org/10.1111/tpj.70115","url":null,"abstract":"Here, we report the successful implementation of heritable virus-induced genome editing (VIGE) in tomato (Solanum lycopersicum). We generated three transgenic tomato lines expressing Streptococcus pyogenes Cas9 (SpCas9) under the control of Cauliflower mosaic virus 35S (35S), S. lycopersicum ribosomal protein S5A (SlRPS5A), or S. lycopersicum YAO promoters (SlYAO). These three lines were tested for somatic and heritable editing using the tobacco rattle virus (TRV)-based system carrying guide RNAs (gRNAs) fused with mobile RNA sequences. TRV with gRNA targeted to Phytoene desaturase (SlPDS) and Downy mildew resistance 6 (SlDMR6) genes fused to mobile RNA sequences showed significant somatic editing efficiency in all three tomato lines expressing SpCas9. However, the progenies from the SlYAO promoter-driven SpCas9 tomato infected with TRV with gRNA targeted to SlDMR6 fused to the mobile RNA sequence resulted in monoallelic mutations with a frequency of 3%. Optimization of environmental conditions, such as reduced light intensity, significantly increased heritable editing frequencies, from 0% to 86% at the SlPDS and from 3% to 100% at the SlDMR6, including biallelic mutations. These findings underscore the use of appropriate promoters to express Cas nucleases and optimized environmental conditions to enhance heritable genome editing efficiency in tomato using VIGE. Furthermore, our method enables the generation of mutants without additional tissue culture or transformation once a SpCas9-expressing tomato line is established.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0