The Plant Journal最新文献_第3页

GhMYB106_D13 coordinates cuticle biosynthesis to build the dual shields for anther protection in upland cotton GhMYB106_D13协调陆地棉角质层生物合成，构建花药保护双护盾

IF 6.2 1区生物学

The Plant Journal Pub Date : 2026-10-07 DOI: 10.1111/tpj.71142

Bin Gao, Meilin Chen, Chao Fu, Xianlong Zhang, Zhongxu Lin

{"title":"GhMYB106_D13 coordinates cuticle biosynthesis to build the dual shields for anther protection in upland cotton","authors":"Bin Gao, Meilin Chen, Chao Fu, Xianlong Zhang, Zhongxu Lin","doi":"10.1111/tpj.71142","DOIUrl":"https://doi.org/10.1111/tpj.71142","url":null,"abstract":"The plant cuticle acts as a crucial barrier against abiotic stresses. In this study, the vulnerable anther (van) gene on chromosome D13 was map-based cloned in upland cotton and identified as GhMYB106_D13. It was demonstrated that the differential distribution of MYB106 between D13 and A13 may represent post-polyploidization convergent evolutionary events. Transcriptome analysis, metabolic profiling, and microscopic examination of anther cuticle in GhMYB106_D13 over-expression and knockout lines revealed that GhMYB106_D13 affects the wax and cuticle biosynthesis pathway. GC-MS analysis demonstrated that the loss of GhMYB106_D13 resulted in an ~27.6% reduction in total wax load, predominantly characterized by a decrease in very-long-chain alkanes, whereas overexpression (OE) led to wax accumulation. Key genes involved in the pathway including FACR2, LACS1, HHT1, and PXG4 were found to be changed dynamically. Specifically, GhMYB106_D13 likely activates the expression of LACS1, a gene involved in wax synthesis that was downregulated in Stage-8 anthers of the Ghmyb106_D13 mutant. Meanwhile, upregulation of FACR2, CYP704B1, and HHT1 was suggested to be potentially involved in cuticle metabolism, possibly through altered 16-feruloyloxypalmitic acid (16-FA) level. Thus, GhMYB106_D13 contributes to the formation of the first protective ‘shield’ for the anther against external stresses. Additionally, GhMYB106_D13 participates in trichome differentiation, which constitutes a second ‘shield’ that encloses the petals and sequesters the anther in the bud before flowering. Overall, these findings unveil the dual function of GhMYB106_D13 in physical protection of plant propagation and offer valuable insights for future strategies aimed at improving stress tolerance in crops.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149099998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Phylogeny, chromosomal mapping and expression analyses of wheat CLAVATA pathway components suggest differential selection on receptor-like kinases, CLEs and T3 WOXes” 更正“小麦CLAVATA通路组分的系统发育、染色体定位和表达分析表明受体样激酶、cle和T3 WOXes存在差异选择”

IF 6.2 1区生物学

The Plant Journal Pub Date : 2026-10-07 DOI: 10.1111/tpj.71165

引用次数: 0

SnRK2.10 in apple positively regulates the nitrogen use efficiency via modulating the nitrogen uptake SnRK2.10通过调控氮素吸收正向调节苹果氮素利用效率。

IF 6.2 1区生物学

The Plant Journal Pub Date : 2026-10-05 DOI: 10.1111/tpj.71139

Ying Ye, Zisheng Zuo, Zhaoting Li, Lin Luo, Xiaojun Huang, Yuduan Ding, Fengwang Ma, Xiaoqing Gong

{"title":"SnRK2.10 in apple positively regulates the nitrogen use efficiency via modulating the nitrogen uptake","authors":"Ying Ye, Zisheng Zuo, Zhaoting Li, Lin Luo, Xiaojun Huang, Yuduan Ding, Fengwang Ma, Xiaoqing Gong","doi":"10.1111/tpj.71139","DOIUrl":"10.1111/tpj.71139","url":null,"abstract":"Sucrose non-fermentation-1–related protein kinase 2 (SnRK2) family members play key roles in plant adaptation to environmental stress. In this study, low nitrogen (N) stress induced the expression of SnRK2.10, which mitigated the adverse effects of N deficiency in apple plants. SnRK2.10-overexpressing (OE) plants showed enhanced growth and higher nitrogen use efficiency (NUE), whereas SnRK2.10-silenced (Ri) plants exhibited more severe growth inhibition and reduced NUE, compared with the wild-type (WT) plants under low N conditions. The transcriptomic and proteomic analyses indicated that SnRK2.10 regulates N metabolism and transport. It physically interacted with the nitrate transporter NRT2.5 and the ammonium transporter AMT1.1. Consistently, OE plants displayed higher NO3− and NH4+ uptake rates than WT and Ri plants under low N stress. Functional analyses using transgenic apple lines with different expression levels of NRT2.5 or AMT1.1 demonstrated that both transporters positively regulate growth and NUE in apple plants' response to low N stress. Co-expression of SnRK2.10 with either NRT2.5 or AMT1.1 effectively altered plant growth under low N stress. Collectively, these results indicate that SnRK2.10 positively regulates plant growth and NUE under low N stress, probably by promoting N uptake through direct interaction with key N transporters.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13639512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NLP6/7-mediated nitrate signaling facilitates high-affinity potassium uptake and enhances tolerance to low potassium environments in Arabidopsis. nlp6 /7介导的硝酸盐信号通路促进拟南芥高亲和钾吸收，增强对低钾环境的耐受性。

IF 6.2 1区生物学

The Plant Journal Pub Date : 2026-10-01 DOI: 10.1111/tpj.71172

Kosuke Usuda, Mailun Yang, Namie Ohtsuki, Nana Sugimura, Daiki Teshima, Yasuhito Sakuraba, Shuichi Yanagisawa

{"title":"NLP6/7-mediated nitrate signaling facilitates high-affinity potassium uptake and enhances tolerance to low potassium environments in Arabidopsis.","authors":"Kosuke Usuda, Mailun Yang, Namie Ohtsuki, Nana Sugimura, Daiki Teshima, Yasuhito Sakuraba, Shuichi Yanagisawa","doi":"10.1111/tpj.71172","DOIUrl":"10.1111/tpj.71172","url":null,"abstract":"Balancing the acquisition of the macronutrients nitrogen (N) and potassium (K) is critical for plant growth, yet the mechanisms that balance N and K acquisition remain poorly understood. Here, we show that the NIN-LIKE PROTEIN7 (NLP7) transcription factor, which mediates nitrate signaling, plays a crucial role in promoting K acquisition in Arabidopsis. K+ uptake activity was reduced in the nlp7 mutant and nlp6nlp7 double mutant but enhanced in the NLP7-overexpressing line. Consistently, these mutants were hypersensitive to K deficiency, whereas the NLP7-overexpressing line displayed increased tolerance. Transcriptome analysis of the nlp6nlp7 mutant suggested that NLP7 activates several critical K-related genes, including HAK5, a transporter gene essential for K+ uptake from soil, and SKOR, a channel gene critical for root-to-shoot transport of K+. Using co-transfection assays, gel electrophoresis mobility shift assays, and chromatin immunoprecipitation assays, we demonstrate that NLP7 directly interacts with and activates the promoters of these two genes. Furthermore, the enhanced K+ uptake and improved K deficiency responses driven by NLP7 overexpression were mitigated in the hak5 and skor mutants, demonstrating the significant physiological role of NLP7-induced activation of these genes. Although HAK5 and SKOR responded differently to K deficiency, both genes were activated similarly by NLP7 under K-sufficient and K-deficient conditions. Together, these findings suggest that K deficiency-induced regulation and nitrate-responsive regulation operate through independent transcriptional mechanisms but collaborate to ensure appropriate K acquisition under diverse N and K conditions, especially when K availability is limiting.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 1","pages":"e71172"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13669645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pepper RING-type E3 ligase, CaANKR1, negatively regulates drought tolerance by modulating CaMAPK1 stability. 辣椒环型E3连接酶CaANKR1通过调节CaMAPK1的稳定性负向调控耐旱性。

IF 6.2 1区生物学

The Plant Journal Pub Date : 2026-10-01 DOI: 10.1111/tpj.71181

Junyoung Cho, Chae Woo Lim, Bomyeon Yun, Dae Sung Kim, Sung Chul Lee

{"title":"Pepper RING-type E3 ligase, CaANKR1, negatively regulates drought tolerance by modulating CaMAPK1 stability.","authors":"Junyoung Cho, Chae Woo Lim, Bomyeon Yun, Dae Sung Kim, Sung Chul Lee","doi":"10.1111/tpj.71181","DOIUrl":"10.1111/tpj.71181","url":null,"abstract":"Drought stress severely restricts plant growth and productivity, and abscisic acid (ABA)-mediated signaling plays a central role in coordinating adaptive responses to water deficit. Mitogen-activated protein kinase (MAPK) cascades are widely involved in plant stress signaling; however, the ubiquitin-mediated regulation of MAPK stability during drought responses remains poorly understood. In this study, we conducted a yeast two-hybrid screening and identified CaMAPK1, a pepper (Capsicum annuum) MAPK, as an interacting partner of the RING-type E3 ubiquitin ligase CaANKR1, which was further confirmed via in vitro and in vivo assays. CaMAPK1 expression was induced by dehydration, osmotic stress, salinity stress, and ABA treatment. Furthermore, CaMAPK1 exhibited kinase activity and was predominantly localized to the nucleus. Ubiquitination assays revealed that CaANKR1 promoted K48-linked polyubiquitination of CaMAPK1 in a RING domain-dependent manner. Consistently, cell-free degradation assays showed that CaMAPK1 protein stability negatively correlated with CaANKR1 expression levels, indicating that CaANKR1 promotes the proteasome-dependent degradation of CaMAPK1. Phenotypic assays revealed that CaMAPK1 silencing in pepper reduced drought tolerance by altering ABA-mediated stomatal responses and water retention, whereas CaMAPK1 overexpression in Arabidopsis produced the opposite phenotype. Collectively, these findings suggest that CaANKR1 negatively modulates ABA-mediated drought tolerance through ubiquitin-dependent destabilization of CaMAPK1. Together, they reveal a mechanism underlying ABA-driven drought responses and highlight a promising target for enhancing crop drought resilience.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 1","pages":"e71181"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13669525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The plant longevity gene AHL15 delays leaf senescence by repressing ORESARA1 and cytokinin degradation. 植物长寿基因AHL15通过抑制ORESARA1和细胞分裂素降解延缓叶片衰老。

IF 6.2 1区生物学

The Plant Journal Pub Date : 2026-10-01 DOI: 10.1111/tpj.71177

Thalia Luden, Petra Amakorová, Ellora Basu, Ondřej Novák, Salma Balazadeh, Remko Offringa

{"title":"The plant longevity gene AHL15 delays leaf senescence by repressing ORESARA1 and cytokinin degradation.","authors":"Thalia Luden, Petra Amakorová, Ellora Basu, Ondřej Novák, Salma Balazadeh, Remko Offringa","doi":"10.1111/tpj.71177","DOIUrl":"10.1111/tpj.71177","url":null,"abstract":"The Arabidopsis thaliana (Arabidopsis) AT-HOOK MOTIF NUCLEAR LOCALIZED 15 (AHL15) gene is associated with various longevity phenotypes and extends the life span of plants when overexpressed. In this study, we show that, in addition to previously described longevity phenotypes, constitutive overexpression of AHL15 in Arabidopsis delays leaf senescence, whereas ahl15 loss of function accelerates this process. Dexamethasone-induced nuclear localization of AHL15-GR during dark-triggered senescence results in a stay-green phenotype and represses the expression of several early senescence-associated genes. Among these, the ORESARA1 (ORE1) locus is directly bound by AHL15, suggesting a direct delaying effect of AHL15 on senescence. Furthermore, we demonstrate that AHL15 acts by directly repressing the expression of several CYTOKININ OXIDASE (CKX) genes involved in cytokinin inactivation, resulting in delayed degradation of cytokinins during dark-induced senescence. Cytokinins are known to inhibit senescence, and this, together with the downregulation of ORE1 expression, explains the delaying effect of AHL15 on senescence.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 1","pages":"e71177"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13669646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A modular UDP-glycosyltransferase and cytochrome P450 system for soyasaponin C-22 modification. 大豆皂苷C-22修饰的模块化udp -糖基转移酶和细胞色素P450系统。

IF 6.2 1区生物学

The Plant Journal Pub Date : 2026-10-01 DOI: 10.1111/tpj.71149

Susumu Hiraga, Yukiko Fujisawa, Panneerselvam Krishnamurthy, Hae-Reon Son, Soo Yeon Chung, Hayato Suzuki, Ryu Nakata, Yoshikazu Shimoda, Ryoichi Yano, Akito Kaga, Chigen Tsukamoto, Hikaru Seki, Toshiya Muranaka, Masao Ishimoto

{"title":"A modular UDP-glycosyltransferase and cytochrome P450 system for soyasaponin C-22 modification.","authors":"Susumu Hiraga, Yukiko Fujisawa, Panneerselvam Krishnamurthy, Hae-Reon Son, Soo Yeon Chung, Hayato Suzuki, Ryu Nakata, Yoshikazu Shimoda, Ryoichi Yano, Akito Kaga, Chigen Tsukamoto, Hikaru Seki, Toshiya Muranaka, Masao Ishimoto","doi":"10.1111/tpj.71149","DOIUrl":"10.1111/tpj.71149","url":null,"abstract":"Soybean (Glycine max (L.) Merr.) produces triterpenoid saponins, called soyasaponins, which are categorised as 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) saponins and group A saponins based on the chemical modifications at the C-22 position of their triterpene skeleton. These C-22 modifications confer different biological functions. While most genes involved in soyasaponin biosynthesis have been identified, the enzymes responsible for conjugation of DDMP or arabinose to the C-22 hydroxyl group remain unclear. This study aimed to identify the enzymes involved in these modifications. Co-expression analysis using known saponin biosynthetic genes identified three candidate UGT genes. Among them, UGT73K5 was essential for DDMP saponin biosynthesis in soybean, as ugt73k5 mutants lacked these compounds. Similarly, the orthologous gene in Lotus japonicus, UGT73K6, was required for DDMP saponin biosynthesis, and the ugt73k6 defect was complemented by soybean UGT73K5. In addition, UGT73BU1, a homologous gene not co-expressed with known saponin biosynthetic genes, was identified as a C-22 arabinosyltransferase involved in group A saponin biosynthesis. In L. japonicus, UGT73K6 is genomically co-localised and co-expressed with a cytochrome P450 gene responsible for C-22 hydroxylation, forming a conserved biosynthetic gene cluster. This organisation suggests coordinated biosynthetic modules underlying DDMP saponin production in legumes.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 1","pages":"e71149"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13673397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149190298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The RcDREB1E-RcMYB28 module improves drought tolerance in Rosa chinensis via RcLAR/RcCORA1-mediated proanthocyanidin biosynthesis and stress responses. RcDREB1E-RcMYB28模块通过RcLAR/ rccora1介导的原花青素生物合成和胁迫响应提高月季耐旱性。

IF 6.2 1区生物学

The Plant Journal Pub Date : 2026-10-01 DOI: 10.1111/tpj.71169

Yingjie Zhou, Linmei Chen, Huanyu Zhang, Yeni Chen, Jingjing Xin, Wen Chen, Yongyi Cui, Yuxiao Shen, Ping Luo

{"title":"The RcDREB1E-RcMYB28 module improves drought tolerance in Rosa chinensis via RcLAR/RcCORA1-mediated proanthocyanidin biosynthesis and stress responses.","authors":"Yingjie Zhou, Linmei Chen, Huanyu Zhang, Yeni Chen, Jingjing Xin, Wen Chen, Yongyi Cui, Yuxiao Shen, Ping Luo","doi":"10.1111/tpj.71169","DOIUrl":"10.1111/tpj.71169","url":null,"abstract":"Drought is widely recognized as one of the most severe abiotic stresses limiting plant growth and productivity. Proanthocyanidins (PAs), as key functional secondary metabolites, are central to enhancing plant tolerance to abiotic stresses. Nevertheless, the specific mechanisms underlying PA-mediated drought resistance in rose remain largely unclear. Herein, we demonstrate that drought-induced PA accumulation constitutes a conserved evolutionary mechanism across the plant kingdom, ranging from unicellular algae to angiosperms. Specifically, according to the drought-responsive transcriptome dataset of Rosa chinensis 'Old Blush', an R2R3-MYB transcription factor, RcMYB28, was identified. Overexpression of RcMYB28 remarkably enhanced drought tolerance and induced a significant increase in PAs accumulation, whereas plants with silenced RcMYB28 displayed the opposite phenotypic characteristics. Specifically, RcMYB28 directly regulated the expression of leucoanthocyanidin reductase (RcLAR) and a cold and drought regulatory-protein encoding gene (RcCORA1), thereby promoting PA synthesis and enhancing the stress response. Moreover, we identified RcDREB1E as a potential upstream transcriptional regulator of RcMYB28. RcDREB1E exerts a positive regulatory effect on drought tolerance and PAs accumulation via RcMYB28 expression. Collectively, our results demonstrate a new transcriptional regulatory module (RcDREB1E-RcMYB28) underlying drought-triggered PAs accumulation, which offers valuable genetic resources for engineering enhanced drought tolerance in rose.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 1","pages":"e71169"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13662611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repeated genetic adaptation under demographic limits in kiwifruit. 重复基因适应人口限制在猕猴桃。

IF 6.2 1区生物学

The Plant Journal Pub Date : 2026-10-01 DOI: 10.1111/tpj.71163

Mengyun Qin, Jingwen Huang, Yufang Shen, Quan Jiang, Na Wei, Xiaohong Yao

{"title":"Repeated genetic adaptation under demographic limits in kiwifruit.","authors":"Mengyun Qin, Jingwen Huang, Yufang Shen, Quan Jiang, Na Wei, Xiaohong Yao","doi":"10.1111/tpj.71163","DOIUrl":"10.1111/tpj.71163","url":null,"abstract":"Population genetic theory predicts that species with narrow geographic distributions experience stronger genetic drift, higher inbreeding, and greater mutational burden than their widespread relatives, yet how demographic history shapes the genomic basis of adaptation remains poorly understood. We hypothesized that (i) restricted-range species exhibit reduced genetic diversity, elevated inbreeding, and greater mutation load owing to smaller long-term effective population sizes; (ii) despite these demographic constraints, shared environmental pressures can still drive repeated adaptation; (iii) repeated adaptation may arise through ancestral standing genetic variation, adaptive introgression, or independent de novo mutations. We tested these hypotheses using two kiwifruit species, Actinidia hemsleyana (narrow-ranged) and Actinidia eriantha (widespread), which differ markedly in demographic history and range size but occupy overlapping environments in subtropical China. We show that demographic contraction and inbreeding have eroded genome-wide diversity in the range-restricted species, accompanied by a substantially higher load of deleterious mutations. Despite these contrasting demographic legacies, both species exhibit repeated genomic responses to shared climatic gradients, with adaptation targeting similar genes. Such repeated adaptation was driven primarily by ancestral standing variation, with smaller contributions from adaptive introgression and only limited evidence for independent de novo mutations. These results reveal that demographic constraints shape genome-wide diversity and mutation load but do not preclude repeated adaptation when shared genetic variation remains available.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 1","pages":"e71163"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13655936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149163861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A bHLH121/URI-ANAC017-MAT1/SAM1 regulatory cascade controls iron homeostasis in Arabidopsis. bHLH121/URI-ANAC017-MAT1/SAM1调控级联调控拟南芥铁稳态。

IF 6.2 1区生物学

The Plant Journal Pub Date : 2026-10-01 DOI: 10.1111/tpj.71157

Chunyan Tu, Qi Wu, Fangwei Yu, Renfang Shen, Xiaofang Zhu

{"title":"A bHLH121/URI-ANAC017-MAT1/SAM1 regulatory cascade controls iron homeostasis in Arabidopsis.","authors":"Chunyan Tu, Qi Wu, Fangwei Yu, Renfang Shen, Xiaofang Zhu","doi":"10.1111/tpj.71157","DOIUrl":"10.1111/tpj.71157","url":null,"abstract":"Iron (Fe) deficiency is a critical environmental constraint limiting plant growth, yield, and quality. Although plants have evolved sophisticated mechanisms to enhance Fe uptake, transport, and utilization, key components of the underlying regulatory network remain unclear. Here, we identify the NAM, ATAF1/2, and CUP-SHAPED COTYLEDON 2 (NAC) transcription factor ANAC017 as an essential regulator of Fe homeostasis in Arabidopsis. ANAC017 expression is strongly induced under Fe deficiency, and loss of ANAC017 function leads to reduced Fe accumulation in both roots and shoots, impaired root elongation, and pronounced leaf chlorosis. Notably, anac017 mutants show excessive Fe retention in the cell wall-particularly within the hemicellulose fraction-under Fe-deficient conditions, suggesting that ANAC017 contributes to the mobilization and reutilization of apoplastic Fe. Mechanistically, ANAC017 functions within the canonical Fe signaling cascade. We show that the core Fe regulator, the BASIC HELIX-LOOP-HELIX (bHLH) transcription factor bHLH121/URI, directly activates ANAC017 transcription, and overexpression of ANAC017 partially rescues the Fe-sensitive phenotype of the bhlh121/uri mutant. Further experiments revealed that the transcription factor ANAC017 binds to the promoter of MAT1/SAM1 (encoding S-adenosylmethionine synthetase), potentially modulating its expression. In conclusion, this study reveals that ANAC017 functions downstream of the bHLH121/URI pathway to regulate Fe homeostasis via two separate mechanisms: facilitating cell wall Fe reutilization and mediating S-adenosylmethionine synthetase metabolism by regulating MAT1/SAM1.","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 1","pages":"e71157"},"PeriodicalIF":6.2,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13669638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0