The Plant Journal最新文献

{"title":"OsPUB9 modulates leaf angle and grain size through the brassinosteroid signaling pathway in rice","authors":"Yonghong Xie,&nbsp;Zhupeng Fan,&nbsp;Xueyan Liang,&nbsp;Kaichong Teng,&nbsp;Zejian Huang,&nbsp;Meiyan Huang,&nbsp;Hong Zhao,&nbsp;Kaizhun Xu,&nbsp;Jianxiong Li","doi":"10.1111/tpj.17230","DOIUrl":"https://doi.org/10.1111/tpj.17230","url":null,"abstract":"<div>\u0000 \u0000 <p>Plant U-box (PUB) E3 ubiquitin ligases are well known for their diverse functions in plant growth and development through physical link to cell signaling hubs to activate regulatory networks. Brassinosteroid (BR) signaling is negatively regulated by a protein kinase GSK2, which interacts with and phosphorylates OsOFP8 (OVATE family protein 8) to regulate BR signaling. Here we identified OsPUB9, a functional E3 ubiquitin ligase, which acts as a negative factor in BR signaling to regulate leaf inclination angle and grain size in rice. OsPUB9 displays autoubiquitination activity and is degraded in response to BR treatment. Interaction with OsUBC13, a rice E2 ubiquitin-conjugating enzyme, suppresses OsPUB9 degradation. OsPUB9 interacts with GSK2, and the interaction reduces autoubiquitination of OsPUB9. Coexpression of OsPUB9 and GSK2 in rice protoplasts suppresses degradation of OsPUB9 but promotes degradation of GSK2. OsPUB9 also interacts with OsOFP8, a positive regulator in BR signaling, and the interaction suppresses degradation of OsPUB9 but facilitates OsOFP8 degradation. Our study reveals that OsPUB9, GSK2, and OsOFP8 form a regulatory network in BR signaling to mediate gene expression, leaf angle, and grain size in rice.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlamydomonas reinhardtii, Volvox carteri and related green algae accumulate ketocarotenoids not in vegetative cells but in zygospores","authors":"Sonja Schwarz,&nbsp;Matthias Bauch,&nbsp;Volker Schmitt,&nbsp;Armin Hallmann,&nbsp;Martin Lohr","doi":"10.1111/tpj.17261","DOIUrl":"https://doi.org/10.1111/tpj.17261","url":null,"abstract":"<p>Zygospores of green alga such as <i>Chlamydomonas reinhardtii</i>, <i>Volvox carteri</i> or <i>Dunaliella salina</i> display a bright orange color indicative of carotenoids, yet there have been no reports on their pigment composition. The genomes of these algae contain genes for homologs of the β-carotene ketolase (BKT) from the well-known astaxanthin producer <i>Haematococcus pluvialis</i>, that were assumed to be pseudogenes, because none of these species has been reported to accumulate astaxanthin or other ketocarotenoids. Here, we show that <i>C. reinhardtii</i> and <i>V. carteri</i> synthesize ketocarotenoids specifically in zygospores. Contrary to the vegetative aplanospores of <i>H. pluvialis</i>, the major ketocarotenoid in zygospores of <i>C. reinhardtii</i> is not astaxanthin but 4-ketolutein. Moreover, the ketocarotenoids in maturing zygospores are not synthesized de novo but from carotenoids of the photosynthetic apparatus liberated by a massive breakdown of thylakoid membranes. In line with this conclusion, incubation of zygospores at 9°C instead of 22°C resulted in a reduced thylakoid breakdown and only low amounts of ketocarotenoids, while the accumulation of storage lipids was less affected. Furthermore, we show the full-length BKT from <i>C. reinhardtii</i> to catalyze the ketolation of both α-carotene and lutein in carotenogenic bacteria. We also detected putative BKT genes in the genomes of various other green algae not yet known to synthesize ketocarotenoids, suggesting a zygospore-specific accumulation of ketocarotenoids to be common among Chlamydomonadales. Our observation that zygospores of <i>C. reinhardtii</i> accumulate ketocarotenoids together with storage lipids sheds light on the physiology of a largely unexplored algal life stage crucial for survival and propagation.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17261","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SDG8 and HUB2 depositing euchromatin histone marks play important roles in meiosis and crossing-over regulation","authors":"Amit Kumar Singh,&nbsp;K.V.S.K. Arjun Chowdary,&nbsp;Wen-Hui Shen","doi":"10.1111/tpj.17241","DOIUrl":"https://doi.org/10.1111/tpj.17241","url":null,"abstract":"<div>\u0000 \u0000 <p>Histone modifications play critical roles in plant growth and development. Crossing-over (CO) during meiosis, which constitutes a fundamental process ensuring sexual transmission of genetic material to the next generation and, meanwhile, generating diversity within species by creating new chromosome/allele combinations, occurs predominantly in euchromatin, which is enriched in active histone marks such as H3K4me3, H3K36me3, and H2Bub1. In plants, it is known that CO hotspots are correlated with H3K4me3 but the role of H3K36me3 and H2Bub1 during meiosis remains elusive so far. Here, we studied the Arabidopsis (<i>Arabidopsis thaliana</i>) <i>sdg8-1</i> and <i>hub2-2</i> mutants impeded in depositing H3K36me3 and H2Bub1, respectively. Chromosome spreading using 4′,6-diamidino-2-phenylindole (DAPI) staining indicated that male meiotic stages are defective in the <i>sdg8-1</i> mutant, and the defect increases synergistically in the <i>sdg8-1hub2-2</i> double mutant. Defects in meiosis, seed formation, and silique length were also observed by RNAi-knockdown of <i>SDG8</i> using the meiosis-specific gene <i>DMC1</i> promoter. This corroborates to support a <i>bona fide</i> role of active histone marks during meiosis and plant reproduction. Using the tetrad-based visual reporter lines and immunostaining with antibodies against HEI10 and ZYP1, it was found that synapsis and pairing of homologous chromosomes are abnormal and CO rate increases in <i>sdg8</i> mutants, pointing to a repressive role of <i>SDG8</i> in Arabidopsis male meiotic homologous recombination.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association analyses reveal both anthropic and environmental selective events during eggplant domestication","authors":"Emmanuel Omondi,&nbsp;Lorenzo Barchi,&nbsp;Luciana Gaccione,&nbsp;Ezio Portis,&nbsp;Laura Toppino,&nbsp;Maria Rosaria Tassone,&nbsp;David Alonso,&nbsp;Jaime Prohens,&nbsp;Giuseppe Leonardo Rotino,&nbsp;Roland Schafleitner,&nbsp;Maarten van Zonneveld,&nbsp;Giovanni Giuliano","doi":"10.1111/tpj.17229","DOIUrl":"https://doi.org/10.1111/tpj.17229","url":null,"abstract":"<p>Eggplant (<i>Solanum melongena</i>) is one of the four most important Solanaceous crops, widely cultivated and consumed in Asia, the Mediterranean basin, and Southeast Europe. We studied the genome-wide association of historical genebank phenotypic data on a genotyped worldwide collection of 3449 eggplant accessions. Overall, 334 significant associations for key agronomic traits were detected. Significant correlations were obtained between different types of phenotypic data, some of which were not obvious, such as between fruit size/yield and fruit color components, suggesting simultaneous anthropic selection for genetically unrelated traits. Anthropic selection of traits like leaf prickles, fruit color, and yield, acted on distinct genomic regions in the two domestication centers (India and Southeast Asia), further confirming the multiple domestication of eggplant. To discriminate anthropic from environmental selection in domestication centers, we conducted a genotype–environment association (GEA) on a subset of georeferenced accessions from the Indian subcontinent. The population structure in this area revealed four genetic clusters, corresponding to a latitudinal gradient, and environmental factors explained 31% of the population structure when the effect of spatial distances was removed. GEA and outlier association identified 305 candidate regions under environmental selection, containing genes for abiotic stress responses, plant development, and flowering transition. Finally, in the Indian domestication center anthropic and environmental selection acted largely independently, and on different genomic regions. These data allow a better understanding of the different effects of environmental and anthropic selection during domestication of a crop, and the different world regions where some traits were initially selected by humans.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17229","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted introduction of premature stop codon in plant mitochondrial mRNA by a designer pentatricopeptide repeat protein with C-to-U editing function","authors":"Nikolay Manavski,&nbsp;Eslam Abdel-Salam,&nbsp;Serena Schwenkert,&nbsp;Hans-Henning Kunz,&nbsp;Andreas Brachmann,&nbsp;Dario Leister,&nbsp;Jörg Meurer","doi":"10.1111/tpj.17247","DOIUrl":"https://doi.org/10.1111/tpj.17247","url":null,"abstract":"<p>RNA editing is a crucial post-transcriptional modification in endosymbiotic plant organelles, predominantly involving C-to-U conversions. Pentatricopeptide repeat (PPR) proteins play a key role in this process. To establish a system for gene expression manipulation in genetically inaccessible mitochondria, we engineered a synthetic PPR protein, dPPR-<i>nad7</i>-DYW, to induce <i>de novo</i> C-to-U editing in the <i>NADH dehydrogenase subunit 7</i> (<i>nad7</i>) mRNA of <i>Arabidopsis thaliana</i>, thereby creating a premature stop codon. This designer protein, composed of 13 P-type PPR domains, was fused with the DYW-type cytidine deaminase domain from <i>Physcomitrium patens</i> PpPPR_56 and programmed to bind a specific <i>nad7</i> mRNA segment. <i>In vitro</i> binding assays confirmed the specificity of dPPR-<i>nad7</i>-DYW for its target sequence. When expressed in Arabidopsis plants, dPPR-<i>nad7</i>-DYW achieved up to 85% editing efficiency at the target site, successfully introducing a premature stop codon in <i>nad7</i> mRNA. This resulted in reduced polysome loading of <i>nad7</i> transcripts and a phenotype characteristic of mitochondrial complex I dysfunction. RNA-sequencing revealed potential off-target editing events, albeit at lower frequencies. Our study demonstrates the successful application of an editing factor with a synthetic P-type PPR tract targeting a <i>de novo</i> editing site in plant mitochondria, achieving high editing efficiency. This approach opens new avenues for manipulating organellar gene expression and studying mitochondrial gene function in plants and other eukaryotes.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17247","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chloroplast-located HKT transporter plays an important role in fertilization and development in Physcomitrium patens","authors":"Carolina Yanez-Dominguez,&nbsp;Karla Macedo-Osorio,&nbsp;Daniel Lagunas-Gomez,&nbsp;Diana Torres-Cifuentes,&nbsp;Juan Castillo-Gonzalez,&nbsp;Guadalupe Zavala,&nbsp;Omar Pantoja","doi":"10.1111/tpj.17253","DOIUrl":"https://doi.org/10.1111/tpj.17253","url":null,"abstract":"<div>\u0000 \u0000 <p>Cell survival depends on the maintenance of cell homeostasis that involves all the biochemical, genomic and transport processes that take place in all the organelles within a eukaryote cell. In particular, ion homeostasis is required to regulate the membrane potential and solute transport across all membranes, any alteration in these parameters will reflect in the malfunctioning of any organelle, and consequently, in the development of the organism. In plant cells, sodium transporters play a central role in keeping the concentrations of this cation across all membranes under physiological conditions to prevent its toxic effects. HKT transporters are a family of membrane proteins exclusively present in plants, with some homologs present in prokaryotes. HKT transporters have been associated to salt tolerance in plants, retrieving any leak of the cation into the xylem, or removing it from aerial parts, including the flowers, to be transported to the roots along the phloem. This function has been assigned as most of the HKT transporters are located at the plasma membrane. Here, we report the localization of the HKT from <i>Physcomitrium patens</i> to the thylakoid membrane, reminiscent of the prokaryote origin of these family of transporters. Mutation of <i>PpHKT</i> leads to several alterations in the phenotype of the organism, including the lack of sporophyte formation, and changes in expression of many genes. These alterations suggest that the breakdown in chloroplast ion homeostasis triggers a signalling cascade to the nucleus to communicate its status, being important for the moss to complete its life cycle.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The heat shock factor HSFB1 coordinates plant growth and drought tolerance in Arabidopsis","authors":"Lanjie Zheng,&nbsp;Qianlong Zhang,&nbsp;Chen Wang,&nbsp;Zhongbao Wang,&nbsp;Jie Gao,&nbsp;Runcong Zhang,&nbsp;Yong Shi,&nbsp;Xu Zheng","doi":"10.1111/tpj.17258","DOIUrl":"https://doi.org/10.1111/tpj.17258","url":null,"abstract":"<div>\u0000 \u0000 <p>Plants are constantly challenged by a diversity of abiotic stressors, and growth arrest is a common plant response aimed at enhancing stress tolerance. Because of this growth/stress tolerance antagonism, plants must finely modulate their growth and responses to environmental stimuli. Here, we demonstrate that HSFB1, a heat shock transcription factor, plays a critical role in the coordination of plant growth and drought stress responses in <i>Arabidopsis thaliana</i>. First, we found that HSFB1 negatively regulates plant growth and development under normal conditions and that <i>HSFB1</i> expression is enhanced under drought stress. Conversely, the loss-of-function mutant <i>hsfb1</i> exhibited increased plant growth and reduced drought stress tolerance compared with the wild-type. Consistently, overexpression of <i>HSFB1</i> suppressed plant growth and enhanced drought stress tolerance. Subsequently, via chromatin immunoprecipitation sequencing, RNA sequencing, and transient expression assays, we screened and identified the heat shock protein 101 (HSP101) gene as a direct transcriptional target of HSFB1. Genetic analysis suggested that <i>HSP101</i> functions downstream of HSFB1 to positively regulate drought tolerance in plants. Furthermore, we found that HSFB1 physically interacts with the eukaryotic translation initiation factor eIF3G1, and this interaction appears to be further enhanced under drought stress. Notably, the mutation of <i>eif3g1</i> increased the severity of drought-induced growth inhibition in the <i>hsfb1</i> mutant, and eIF3G1 enhanced the transcriptional activation of HSFB1 on the <i>HSP101</i> promoter under drought stress. Altogether, our findings highlight HSFB1 as a key regulator coordinating plant growth and drought stress responses in Arabidopsis.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma membrane H+-ATPase activation increases global transcript levels and promotes the shoot growth of light-grown Arabidopsis seedlings","authors":"Satoru Naganawa Kinoshita,&nbsp;Kyomi Taki,&nbsp;Fumika Okamoto,&nbsp;Mika Nomoto,&nbsp;Koji Takahashi,&nbsp;Yuki Hayashi,&nbsp;Junko Ohkanda,&nbsp;Yasuomi Tada,&nbsp;Iris Finkemeier,&nbsp;Toshinori Kinoshita","doi":"10.1111/tpj.70034","DOIUrl":"https://doi.org/10.1111/tpj.70034","url":null,"abstract":"<p>Plant cell growth requires the elongation of cells mediated by cell wall remodelling and turgor pressure changes. The plasma membrane (PM) H⁺-ATPase facilitates both cell wall loosening and turgor pressure changes by acidifying the apoplast of cells, referred to as acid growth. The acid growth theory is mostly established on the auxin-induced activation of PM H⁺-ATPase in non-photosynthetic tissues. However, how PM H⁺-ATPase affects the growth in photosynthetic tissues of Arabidopsis remains unclear. Here, a combination of transcriptomics and cis-regulatory element analysis was conducted to identify the impact of PM H⁺-ATPase on global transcript levels and the molecular mechanism downstream of the PM H⁺-ATPase. The PM H⁺-ATPase activation increased transcript levels globally, especially cell wall modification-related genes. The transcript level changes were in PM H⁺-ATPase-dependent manner. Involvement of Ca²⁺ was suggested as CAMTA motif was enriched in the promoter of PM H⁺-ATPase-induced genes and cytosolic Ca²⁺ elevated upon PM H⁺-ATPase activation. PM H⁺-ATPase activation in photosynthetic tissues promotes the expression of cell wall modification enzymes and shoot growth, adding a novel perspective of photosynthesis-dependent PM H⁺-ATPase activation in photosynthetic tissues to the acid growth theory that has primarily based on findings from non-photosynthetic tissues.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic reprogramming underlying anatomical and physiological characteristics of poplar wood in acclimation to changing light and nitrogen availabilities","authors":"Yan Lu,&nbsp;Shurong Deng,&nbsp;Jiangting Wu,&nbsp;Hong Li,&nbsp;Jing Zhou,&nbsp;Wenguang Shi,&nbsp;Payam Fayyaz,&nbsp;Zhi-Bin Luo","doi":"10.1111/tpj.17234","DOIUrl":"https://doi.org/10.1111/tpj.17234","url":null,"abstract":"<div>\u0000 \u0000 <p>To explore the proteomic regulation that underlies the physiological, anatomical, and chemical characteristics of wood in acclimation to changing light and nitrogen (N), saplings of <i>Populus</i> × <i>canescens</i> were treated with control or high irradiance in combination with low, control or high N for 4 months. High irradiance led to elevated levels of starch, sucrose, glucose, and fructose, decreased concentrations of ammonium, nitrate, most amino acids and total N, wider xylem, more xylem cell layers, narrower vessel lumina, longer fiber cells, greater fiber wall thickness, and more cellulose and hemicellulose but less lignin deposition in poplar wood. Limiting N resulted in increased levels of starch and sucrose, reduced levels of glucose, fructose, ammonium, nitrate, amino acids and total N, narrower xylem, fewer xylem cell layers, reduced vessel lumen diameter, thicker fiber walls, and less cellulose and more hemicellulose and lignin accumulation, whereas high N had the opposite effects on poplar wood. Correspondingly, numerous differentially abundant proteins, which are related mainly to the metabolism of carbohydrates and amino acids, cell division and expansion, and deposition of secondary cell walls, such as sucrose synthase 6 (SUS6), cell division cycle protein 48 (CDC48) and laccases (LACs), were identified in poplar cambiums in response to changes in light intensity and N availability. These results suggest that proteomic relays play essential roles in regulating the physiological characteristics and anatomical and chemical properties of poplar wood in acclimation to changing light and N availabilities.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lysine acetylation modulates drought stress responses in birch (Betula platyphylla) through metabolic and transcriptional pathway regulation","authors":"Shilin Sun,&nbsp;Xue Han,&nbsp;Jiayi Wang,&nbsp;Mingshuang Liu,&nbsp;Siqi Wu,&nbsp;Di Wu,&nbsp;Yucheng Wang","doi":"10.1111/tpj.17260","DOIUrl":"https://doi.org/10.1111/tpj.17260","url":null,"abstract":"<div>\u0000 \u0000 <p>Acetylation modifies protein subcellular localization, stability, enzymatic activity, and protein–protein and protein-DNA interactions, playing a crucial role in mediating protein function. However, research on non-histone acetylation remains limited. This study investigates changes in lysine acetylation (Kac) in proteins of birch (<i>Betula platyphylla</i>) in response to drought using 4D label-free quantitative lys-acetylproteome analysis. We identified a total of 15 064 acetylated peptides across 4393 proteins, with 2486 Kac sites exhibiting significant changes: 246 prteins showed increased Kac levels, while 1406 displayed reductions. Notably, proteins associated with metabolic pathways, such as nucleotide sugar biosynthesis, proteasome, glutamate decarboxylase and the tricarboxylic acid (TCA) cycle, were significantly impacted. The alterations in Kac levels correlated with various KEGG pathways, suggesting that acetylation plays a regulatory role in drought response mechanisms. Furthermore, we identified specific acetylation sites in transcription factors (TFs), highlighting their involvement in this process. Functional validation demonstrated that mutations in Kac sites of five randomly selected TFs resulted in significant changes in drought tolerance, emphasizing the critical role of lysine acetylation in modulating stress responses. Overall, our findings indicate that Kac modification serves as a key regulatory mechanism in birch adaptation to drought stress, influencing both metabolic processes and transcriptional regulation.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}