The Plant Journal最新文献

{"title":"SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato","authors":"Qianwen Zhang,&nbsp;Yue Liu,&nbsp;Chunli Zhang,&nbsp;Dawei Xu,&nbsp;Ana L. Medina-Fraga,&nbsp;Baoguo Wu,&nbsp;Chenyang Guo,&nbsp;MeLongying Wangzha,&nbsp;Guoqian Yang,&nbsp;Danmeng Zhu,&nbsp;David Weiss,&nbsp;Carlos L. Ballaré,&nbsp;Li Lin,&nbsp;Ruohe Yin","doi":"10.1111/tpj.17135","DOIUrl":"10.1111/tpj.17135","url":null,"abstract":"<div>\u0000 \u0000 <p>Tomato (<i>Solanum lycopersicum</i> L.) is an important model plant species in photomorphogenesis research. Ultraviolet B (UV-B) induces the dissociation of homodimers of the photoreceptor UV RESISTANCE LOCUS8 (UVR8) into monomers, which translocate into the nucleus. Nuclear accumulation of UVR8 is a prerequisite for its signaling function. Previous studies have reported that SUPPRESSOR OF PHYTOCHROME A-105 (SPA) family members may regulate UV-B signaling in Arabidopsis (<i>Arabidopsis thaliana</i>); however, the underlying mechanism is unknown. Here, we show that the tomato genome encodes four SPA (SlSPA) orthologs. Genome-edited <i>Slspa3</i> mutants exhibited enhanced photomorphogenic responses in white light, suggesting that SlSPA3 inhibits general photomorphogenesis. By contrast, UVR8-mediated gene expression in response to UV-B was compromised in <i>Slspa3</i> mutants, suggesting that SlSPA3 promotes UV-B signaling. UV-B-induced nuclear accumulation of UVR8, which is essential for UV-B signaling, was reduced in the <i>Slspa3</i> mutants. Moreover, UV-B-induced nuclear accumulation of UVR8 was also reduced in the Arabidopsis <i>spa1 spa2 spa3</i> and <i>spa1 spa2 spa4</i> triple mutants, indicating a conserved mechanism in these two species. Notably, <i>spa1 spa2 spa4</i> exhibited normal UV-B-induced interaction between UVR8 and the plant morphogenesis repressor CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1). This suggests that the well-established mechanisms of UVR8 nuclear retention remained unaffected in <i>spa1 spa2 spa4</i>. Thus, our work uncovered a potentially unrecognized mechanism by which SPA proteins regulate UV-B signaling through the promotion of UVR8 nuclear abundance in land plants.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2656-2667"},"PeriodicalIF":6.2,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GhDOFD45 promotes sucrose accumulation in cotton seeds by transcriptionally activating GhSWEET10 expression","authors":"Chuanhui Du,&nbsp;Wenjie Sun,&nbsp;Qingwei Song,&nbsp;Kaijing Zuo","doi":"10.1111/tpj.17123","DOIUrl":"10.1111/tpj.17123","url":null,"abstract":"<div>\u0000 \u0000 <p>Cotton seed development and fiber elongation are the inseparable and overlapped development processes requiring the continuous supply of sucrose as the direct carbon source. However, little is known about the molecular mechanism of how sucrose is transported from the source tissues (leaves) into growing cotton seeds. Here, we identify the function of a sucrose transporter gene, <i>Sugars Will Eventually be Exported Transporter 10</i>, <i>GhSWEET10</i> in cotton seed development. <i>GhSWEET10</i> encodes a functional sucrose transporter, predominantly expressing in the funiculus, inner seedcoat, and endosperm during fiber elongation. <i>GhSWEET10</i> RNAi plants (<i>GhSWEET10i</i>) accumulated less sucrose and glucose in growing seeds and that led to shorter fibers and smaller seeds, whereas <i>GhSWEET10</i> overexpressed plants (<i>GhSWEET10OE</i>) had bigger seeds and longer fibers with more sugar accumulation during fiber elongation. <i>GhSWEET10</i> gene is transcriptionally controlled by the transcription factor GhDOFD45. <i>GhDOFD45</i> knockout plants (<i>GhDOFD45-KO</i>) possessed the phenotypes of smaller seeds and shorter fibers like those of <i>GhSWEET10i</i> plants. Furthermore, GhSWEET10 mainly exports the sucrose from the funiculus into developing seeds according to the mimic-analysis of sucrose transporting. Collectively, all these findings show that GhDOFD45 positively regulates <i>GhSWEET10</i> expression to mainly transport sucrose from leaves into developing cotton seeds. Our findings also imply that the sucrose transport into enlarging seeds benefits fiber development, and thus <i>GhSWEET10</i> can be selected as a target of breeding novel cotton varieties with larger and more vigorous seeds.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2468-2484"},"PeriodicalIF":6.2,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsbHLH6, a basic helix–loop–helix transcription factor, confers arsenic tolerance and root-to-shoot translocation in rice","authors":"Menghan Huang,&nbsp;Yang Liu,&nbsp;Qianwen Bian,&nbsp;Wenjing Zhao,&nbsp;Juan Zhao,&nbsp;Qingpo Liu","doi":"10.1111/tpj.17124","DOIUrl":"10.1111/tpj.17124","url":null,"abstract":"<div>\u0000 \u0000 <p>Arsenic (As) is extremely toxic to plants, posing a serious concern for food safety. Identification of genes responsive to As is significative for figuring out this issue. Here, we identified a bHLH transcription factor OsbHLH6 that was involved in mediating the processes of As tolerance, uptake, and root-to-shoot translocation in rice. The expression of <i>OsbHLH6</i> gene was strongly induced after 3 and 48 h of arsenite [As(III)] treatment. The <i>OsbHLH6</i>-overexpressed transgenic rice (OE-<i>OsbHLH6</i>) was sensitive to, while the knockout mutant of <i>OsbHLH6</i> gene (<i>Osbhlh6</i>) was tolerant to As(III) stress by affecting the contents of reactive oxygen species (ROS) and non-protein thiols (NPT), etc. Knockout of <i>OsbHLH6</i> gene increased significantly the As concentration in roots, but decreased extensively As accumulation in shoots, compared to that in OE-<i>OsbHLH6</i> and WT plants. The transcripts of phytochelatins (PCs) synthetase encoding genes <i>OsPCS1</i> and <i>OsPCS2</i>, as well as As(III) transporter encoding genes <i>OsLsi1</i> and <i>OsABCC1</i> were greatly abundant in <i>Osbhlh6</i> mutants than in OE-<i>OsbHLH6</i> and WT plants under As(III) stress. In contrast, the expression of <i>OsLsi2</i> gene was extensively suppressed by As(III) in <i>Osbhlh6</i> mutants. OsbHLH6 acted as a transcriptional activator to bind directly to the promoter and regulate the expression of <i>OsPrx2</i> gene that encodes a peroxidase precursor. Moreover, overexpression of <i>OsbHLH6</i> gene resulted in significant change of expression of amounts of abiotic stress-related genes, which might partially contribute to the As sensitivity of OE-<i>OsbHLH6</i> plants. These findings may broaden our understanding of the molecular mechanism of <i>OsbHLH6</i>-mediated As response in rice and provide novel useful genes for rice As stress-resistant breeding.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2485-2499"},"PeriodicalIF":6.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosystem rearrangements, photosynthetic efficiency, and plant growth in far red-enriched light","authors":"Maïté Leschevin,&nbsp;Brigitte Ksas,&nbsp;Raymonde Baltenweck,&nbsp;Philippe Hugueney,&nbsp;Stefano Caffarri,&nbsp;Michel Havaux","doi":"10.1111/tpj.17127","DOIUrl":"10.1111/tpj.17127","url":null,"abstract":"<p>Arabidopsis plants were grown in white light (400–700 nm) or in white light supplemented with far-red (FR) light peaking at 730 nm. FR-enriched light induced the typical shade avoidance syndrome characterized by enhanced length of seedling hypocotyl and leaf petiole. FR supplementation also caused a noticeable decrease in the carotenoid and chlorophyll content that was attributable to a block of pigment accumulation during plant development. The carotenoid decrease resulted from a downregulation of their biosynthesis pathway rather than carotenoid degradation. The losses of photosynthetic pigments are part of structural and functional rearrangements of the photosynthetic apparatus. The plastoquinone pool was chronically more oxidized in plants acclimated to white + FR light compared to white light-grown plants. Growth in FR-enriched light was associated with a higher photochemical efficiency of PSII compared to growth in white light and with a substantial increase in root and shoot biomass production. Light distribution between the photosystems was modified in favor of PSII by an increase in the PSII/PSI ratio and an inhibition of state transitions. Neither LHCII abundance nor nonphotochemical energy dissipation in the PSII chlorophyll antennae were modified significantly by the addition of FR light. A PSI supercomplex, not previously observed in Arabidopsis, was specifically found in plants grown in FR-enriched light. This large PSI complex contains a supplementary Lhca1-4 dimer, leading to a total of 6 LHCI antennae instead of 4 in the canonical PSI. Through those photosystem rearrangements and the synergistic interaction with white light, FR light is photosynthetically active and can boost photosynthesis and plant growth.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2536-2552"},"PeriodicalIF":6.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tomato MADS-RIN regulates GAME5 expression to promote non-bitter glycoalkaloid biosynthesis in fruit","authors":"Yinhuan Xie,&nbsp;Yaping Xu,&nbsp;Huimin Jia,&nbsp;Ke Wang,&nbsp;Siyu Chen,&nbsp;Ting Ma,&nbsp;Yuanwei Deng,&nbsp;Zhaobo Lang,&nbsp;Qingfeng Niu","doi":"10.1111/tpj.17125","DOIUrl":"10.1111/tpj.17125","url":null,"abstract":"<div>\u0000 \u0000 <p>A well-known defense-associated steroidal glycoalkaloid (SGA) metabolic shift eliminates the bitterness and toxicity of ripe tomato fruits. This study was conducted to clarify the effects of MADS-RIN (RIN) and its cofactors on SGA metabolism in tomato fruits. Using a CRISPR/Cas9-based gene-editing system, we mutated <i>RIN</i> and two cofactor genes (<i>FUL1</i> and <i>FUL2</i>). The observed changes to fruit color and size in the mutants reflected the overlapping and distinct effects of RIN, FUL1, and FUL2 on fruit ripening. According to a UPLC-MS/MS analysis, the <i>RIN</i> and cofactor mutants had decreased levels of the relatively non-toxic metabolite esculeoside A, but they accumulated toxic SGA pathway intermediates, suggesting RIN and its cofactors are directly involved in esculeoside A biosynthesis. Transcriptome and qPCR analyses detected the downregulated expression of <i>GAME5</i>, which encodes a key enzyme mediating esculeoside A biosynthesis. ChIP-seq and ChIP-qPCR analyses confirmed <i>GAME5</i> is targeted by RIN. RIN was observed to activate <i>GAME5</i> transcription by binding to two non-canonical CArG-boxes in the <i>GAME5</i> promoter. Additionally, RIN promotes SGA metabolism independently of ethylene. Collectively, these findings enhance our understanding of the molecular mechanism governing tomato fruit ripening and SGA biosynthesis. Furthermore, they may be useful for improving tomato fruit quality and safety.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2500-2514"},"PeriodicalIF":6.2,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TaWRKY24 integrates the tryptophan metabolism pathways to participate in defense against Fusarium crown rot in wheat","authors":"Xing Xu,&nbsp;Tai-Fei Yu,&nbsp;Ji-Tong Wei,&nbsp;Xiao-Fei Ma,&nbsp;Yong-Wei Liu,&nbsp;Jin-Peng Zhang,&nbsp;Lei Zheng,&nbsp;Ze-Hao Hou,&nbsp;Jun Chen,&nbsp;Yong-Bin Zhou,&nbsp;Ming Chen,&nbsp;Jian Ma,&nbsp;Yun-Feng Jiang,&nbsp;Hu-Tai Ji,&nbsp;Li-Hui Li,&nbsp;You-Zhi Ma,&nbsp;Zhi-An Zhang,&nbsp;Zhao-Shi Xu","doi":"10.1111/tpj.17079","DOIUrl":"10.1111/tpj.17079","url":null,"abstract":"<div>\u0000 \u0000 <p>Wheat growth process has been experiencing severe challenges arising from the adverse environment. Notably, the incidence of Fusarium crown rot (FCR), a severe soil-borne disease caused by <i>Fusarium pseudograminearum</i> (<i>Fp</i>), has significantly intensified in various wheat-growing regions, resulting in a decline in grain yield. However, the identification of wheat varieties and the exploration of effective gene resources resistant to FCR have not yet been accomplished. Here, we screened and identified the tryptophan metabolism pathway to participate in wheat resistance to FCR by correlation analysis between transcriptome and metabolome, and found that indole-3-acetaldehyde (IAAld) and melatonin, two key metabolites in the tryptophan metabolic pathway, were significantly accumulated in <i>Fp-</i>induced wheat stem bases. Interestingly, exogenous application of these two metabolites could significantly enhance wheat resistance against <i>Fp</i>. Additionally, we observed that the activity of <i>TaALDHase</i>, a crucial enzyme responsible for catalyzing IAAld to produce indole-3-acetic acid (IAA), was inhibited. Conversely, the activity of <i>TaMTase</i>, a rate-limiting involved in melatonin biosynthesis, was enhanced in the <i>Fp</i>-induced wheat transcriptome. Further analysis showed that <i>TaWRKY24</i> could regulate IAA and melatonin biosynthesis by inhibiting the expression of <i>TaALDHase</i> and enhancing the transcription of <i>TaMTase</i>, respectively. Silencing of <i>TaALDHase</i> could significantly increase wheat resistance to FCR. However, interference with <i>TaWRKY24</i> or <i>TaMTase</i> could decrease wheat resistance to FCR. Collectively, our findings demonstrate the crucial role of the tryptophan metabolism pathway in conferring resistance against FCR in wheat, thereby expanding its repertoire of biological functions within the plant system.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 5","pages":"1764-1785"},"PeriodicalIF":6.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Genotypic and phenotypic characterization of a large, diverse population of maize near-isogenic lines","authors":"","doi":"10.1111/tpj.17078","DOIUrl":"10.1111/tpj.17078","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <p><b>RETRACTION:</b> L. Morales, A. C. Repka, K. L. Swarts, W. C. Stafstrom, Y. He, S. M. Sermons, Q. Yang, L. O. Lopez-Zuniga, E. Rucker, W. E. Thomason, R. J. Nelson, P. J. Balint-Kurti. “Genotypic and phenotypic characterization of a large, diverse population of maize near-isogenic lines,” <i>The Plant Journal</i> 103, no. 3 (2020): 1246–1255. https://doi.org/10.1111/tpj.14787.</p>\u0000 \u0000 <p>The above article, published online on 29 April 2020, in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors; the journal Editor-in-Chief, Katherine Denby; Society for Experimental Biology (SEB); and John Wiley &amp; Sons Ltd. The retraction has been agreed following a report submitted by the authors indicating that the imputation of genotypes outlined in the article caused errors in the reported positions of introgressions in the lines. The authors also declared that some of the pedigree information is inaccurate. As a result, all authors agree that the paper must be retracted. The authors have stated their intention to publish a corrected report with improved analyses of introgression positions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 4","pages":"1693"},"PeriodicalIF":6.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ancient duplications, multidimensional specializations, and defense role of hexokinases in wheat","authors":"Xiaolin Tian,&nbsp;Fan Li,&nbsp;Jie Lin,&nbsp;Yun Xu,&nbsp;Kai Tian,&nbsp;Lihua Gu,&nbsp;Yanfeng Zhang,&nbsp;Jin-Rong Xu,&nbsp;Qinhu Wang","doi":"10.1111/tpj.17122","DOIUrl":"10.1111/tpj.17122","url":null,"abstract":"<div>\u0000 \u0000 <p>Hexokinases (HXKs), which sense and catalyze cellular sugar, play a critical role in the growth and development of various plants, including wheat, a primary source of human calories frequently attacked by fungal pathogens. However, the evolutionary dynamics and functional diversification of HXKs in wheat, particularly their roles in plant defense, remain unclear. Here, we discovered that the wheat hexokinase gene family originated through multiple ancient gene duplications across different plant lineages and has undergone comprehensive, multidimensional functional specialization in gene expression, subcellular localization, enzyme activity, and regulation of plant defense responses. Gene expression analysis suggests that two-thirds of the <i>TaHXK</i> genes are responsive to fungal infection. Subcellular analysis reveals that while six TaHXKs are localized in mitochondria, three TaHXKs from different phylogenetic branches are sorted into other cellular compartments. Notably, biochemical analysis shows that TaHXKs in mitochondria differ in their glucose-catalyzing activity, with TaHXK5 and TaHXK3 exhibiting the highest and lowest enzyme activity, respectively. Consistently, transient expression analysis suggests that TaHXK5 induces various plant defense responses, while TaHXK3 is defective in activating some plant defense responses. Furthermore, inactivation of the glucokinase activity of TaHXK5 compromised its function in defense activation, suggesting that mitochondrial TaHXKs display functional divergence in both enzyme activity and defense-inducing activity that are intrinsically connected. Overall, our findings reveal that the multidimensional specialization events following the ancient duplication events may have shaped the functional diversity of HXKs in wheat, shedding light on their evolutionary dynamics and potentially contributing to the improvement of wheat defense.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2456-2467"},"PeriodicalIF":6.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A plastidial lipoyl synthase LIP1p plays a crucial role in phosphate homeostasis in Arabidopsis","authors":"Shenghong Ge,&nbsp;Xinlong Xiao,&nbsp;Ke Zhang,&nbsp;Changhong Yang,&nbsp;Jinsong Dong,&nbsp;Keying Chen,&nbsp;Qiuyu Lv,&nbsp;Viswanathan Satheesh,&nbsp;Mingguang Lei","doi":"10.1111/tpj.17117","DOIUrl":"10.1111/tpj.17117","url":null,"abstract":"<div>\u0000 \u0000 <p>Phosphate (Pi) homeostasis is important for plant growth and adaptation to the dynamic environment, which requires the precise regulation of phosphate transporter (PHT) trafficking from the endoplasmic reticulum to the plasma membrane. LIPOYL SYNTHASE 1p (LIP1p) is known as a key enzyme in plastids to catalyze lipoylation of pyruvate dehydrogenase complex for de novo fatty acid synthesis. It is unknown whether this process is involved in regulating Pi homeostasis. Here, we demonstrate a new role of LIP1p in controlling Pi homeostasis by regulating PHT1 trafficking. We recovered a weak mutant allele of <i>LIP1p</i> in <i>Arabidopsis</i> that accumulates much less Pi and has enhanced expression of phosphate starvation-induced genes. <i>LIP1p</i> mutation alters the lipid profile and compromises vesicle trafficking of PHT1 to the plasma membrane to impair Pi uptake. Beside phosphorus, the homeostasis of a series of mineral nutrients was also perturbed in <i>lip1p</i> mutant. Our findings provide powerful genetic evidence to support the linkage between lipoylation and ion homeostasis in plants.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2385-2397"},"PeriodicalIF":6.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dynamic N1-methyladenosine RNA methylation provides insights into the tomato fruit ripening","authors":"Lili Ma,&nbsp;Jinhua Zuo,&nbsp;Chunmei Bai,&nbsp;Anzhen Fu,&nbsp;Qing Wang,&nbsp;Zhongjing Zhou,&nbsp;Zhiping Deng,&nbsp;Jinjuan Tan,&nbsp;Mondher Bouzayen,&nbsp;Yanyan Zheng","doi":"10.1111/tpj.17095","DOIUrl":"10.1111/tpj.17095","url":null,"abstract":"<div>\u0000 \u0000 <p>N¹-methyladenosine (m¹A) methylation is an essential mechanism of gene regulation known to impact several biological processes in living organisms. However, little is known about the abundance, distribution, and functional significance of mRNA m¹A modification during fruit ripening of tomato the main model species for fleshy fruits. Our study shows that m¹A modifications are prevalent in tomato mRNA and are detected in lncRNA and circRNA. The distribution of m¹A peaks in mRNA segments indicates that m¹A is mainly enriched at the start codon and CDS regions. Assessing changes in global RNA methylation during fruit ripening in wild-type tomatoes and in the ripening-impaired <i>Nr</i> mutant affected in the ethylene receptor gene (<i>SlETR3</i>) revealed a decrease in the overall methylation levels from mature green (MG) stage to 6 days postbreaker (Br + 6). <i>Nr</i> mutant fruits show significantly lower methylation levels than Ailsa Craig (AC) fruits. Notably, differences in m¹A methylation are well correlated to the expression levels of a number of key ripening-related genes. The integration of RNA-seq and MeRIP-seq data suggests a potential positive impact of m¹A modifications on gene expression. In comparison to the AC fruits, the hypomethylation and reduced expression of ethylene-related genes, <i>ACO3</i>, <i>EBF1</i>, and <i>ERF.D6</i>, in the <i>Nr</i> mutants likely underpin the distinct phenotypic traits observed between the two fruit genotypes at the Br6 stage. Overall, our study brings further arguments supporting the potential significance of m¹A methylation modifications in fruit ripening, a developmental process that is instrumental to plant reproduction and to fruit sensory and nutritional qualities.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 5","pages":"2014-2030"},"PeriodicalIF":6.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}