The Plant Journal最新文献

{"title":"OsGSK1 interacts with OsbZIP72 to regulate salt response in rice","authors":"Xi Liu,&nbsp;Xin Guo,&nbsp;Tingjing Li,&nbsp;Xue Wang,&nbsp;Yulu Guan,&nbsp;Di Wang,&nbsp;Yinjie Wang,&nbsp;Xiaonan Ji,&nbsp;Qingsong Gao,&nbsp;Jianhui Ji","doi":"10.1111/tpj.70112","DOIUrl":"https://doi.org/10.1111/tpj.70112","url":null,"abstract":"<div>\u0000 \u0000 <p>Soil salinity remains a continuing threat to agriculture worldwide, greatly affecting seedling development and reducing crop yield. Thus, the cultivation of salt-resistant crops on salinized land is an excellent strategy to ensure food security. The rice GSK3-like protein kinase, OsGSK1, is known to play a role in the response to various abiotic stressors; however, the underlying molecular mechanism of this response remains unclear. Here, we aimed to elucidate the mechanism by which OsGSK1 regulates the salt stress response. We found that OsGSK1 interacts with OsbZIP72 to negatively regulate salt stress tolerance in rice plants. <i>OsGSK1</i> is specifically induced by cold, salt stress, and abscisic acid (ABA) treatment. OsGSK1 was found to be localized in the nucleus and cytoplasm, where it physically interacts with OsbZIP72 – a positive regulator of the rice salt stress response. OsbZIP72 directly binds to the ABA response element in the <i>OsNHX1</i> promoter to regulate its expression under salt stress, whereas OsGSK1 interacts with OsbZIP72 to repress <i>OsNHX1</i> expression. The knockout of <i>OsGSK1</i> increased salt tolerance without affecting the main agronomic traits of the mutant plants. Therefore, OsGSK1 could be used to maintain rice yield in salinized soil.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient targeted T-DNA integration for gene activation and male germline-specific gene tagging in Arabidopsis","authors":"Peng Xu,&nbsp;Jilei Huang,&nbsp;Xiaojing Chen,&nbsp;Qi Wang,&nbsp;Bo Yin,&nbsp;Qing Xian,&nbsp;Chuxiong Zhuang,&nbsp;Yufei Hu","doi":"10.1111/tpj.70104","DOIUrl":"https://doi.org/10.1111/tpj.70104","url":null,"abstract":"<div>\u0000 \u0000 <p>Site-specific DNA integration is an important tool in plant genetic engineering. Traditionally, this process relies on homologous recombination (HR), which is known for its low efficiency in plant cells. In contrast, <i>Agrobacterium</i>-mediated T-DNA integration is highly efficient for plant transformation. However, T-DNA is typically inserted randomly into double-strand breaks within the plant genome via the non-homologous end-joining (NHEJ) DNA repair pathway. In this study, we developed an approach of CRISPR/Cas9-mediated targeted T-DNA integration in Arabidopsis, which was more rapid and efficient than the HR-mediated method. This targeted T-DNA integration aided in gene activation and male germline-specific gene tagging. Gene activation was accomplished by positioning the CaMV35S promoter at the left border of T-DNA, thereby activating specific downstream genes. The activation of <i>FT</i> and <i>MYB26</i> significantly increased their transcriptional expression, which resulted in early flowering and an altered pattern of cell wall thickening in the anther endothelium, respectively. Male germline-specific gene tagging incorporates two reporters, namely, <i>NeoR</i> and <i>MGH3::mCherry</i>, within the T-DNA. This design facilitates the creation of insertional mutants, simplifies the genetic analysis of mutated alleles, and allows for cellular tracking of male germline cells during fertilization. We successfully applied this system to target the male germline-specific gene <i>GEX2.</i> In conclusion, our results demonstrated that site-specific integration of DNA fragments in the plant genome can be rapidly and efficiently achieved through the NHEJ pathway, making this approach broadly applicable in various contexts.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"StNF-YA8-YB20-YC5 module regulates potato tuber dormancy by modulating gibberellin and abscisic acid pathways","authors":"Xiao Wang,&nbsp;Han Wei,&nbsp;Ning Zhang,&nbsp;Shigui Li,&nbsp;Huaijun Si","doi":"10.1111/tpj.70106","DOIUrl":"https://doi.org/10.1111/tpj.70106","url":null,"abstract":"<div>\u0000 \u0000 <p>The molecular mechanisms involved in the regulation of potato tuber dormancy are complex, involving a variety of related genes and enzymes, which modulate multiple signaling pathways. Nuclear factor-Y (NF-Y) transcription factors (TFs) are widely found in eukaryotes and are involved in the regulation of plant embryonic development, seed germination, fruit ripening, and in response to biotic and abiotic stress. Previously, we found that StNF-YA8 gene expression was increasing with the release of potato tuber dormancy. In this study, it was found that <i>StNF-YA8</i> overexpressed tubers broke dormancy earlier than non-transgenic (NT) and <i>StNF-YA8</i> downregulated tubers. Changes in abscisic acid (ABA) and gibberellin (GA) content of different types of tubers at different dormancy periods confirmed that both GA and ABA hormones influenced the differences in dormancy time. This was confirmed by the expression of GA pathway genes <i>StGA3ox1</i> and <i>StGA20ox1</i> genes and ABA pathway genes <i>StCYP707A2</i> and <i>StPP2CA1</i> genes in different tubers. The four genes described above were further shown to be target genes of the StNF-YA8 TF, which transcriptionally activates the expression of these genes. In addition, we verified the involvement of StNF-YA8 in the tuber dormancy release process by the interacting proteins StNF-YB20 and StNF-YC5, which are able to bind to the StNF-YA8-B20-C5 module to activate the transcription of GA and ABA pathway genes. Our study reveals the StNF-YA8-C5 module activates the transcription of the <i>StCYP707A2</i>, <i>StPP2CA1</i>, <i>StGA3ox1</i>, and <i>StGA20ox1</i> genes and alters GA and ABA content, accelerating the release of dormancy in potato tubers.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond a few bases: methods for large DNA insertion and gene targeting in plants","authors":"Katie Vollen,&nbsp;Jose M. Alonso,&nbsp;Anna N. Stepanova","doi":"10.1111/tpj.70099","DOIUrl":"https://doi.org/10.1111/tpj.70099","url":null,"abstract":"<p>Genome editing technologies like CRISPR/Cas have greatly accelerated the pace of both fundamental research and translational applications in agriculture. However, many plant biologists are functionally limited to creating small, targeted DNA changes or large, random DNA insertions. The ability to efficiently generate large, yet precise, DNA changes will massively accelerate crop breeding cycles, enabling researchers to more efficiently engineer crops amidst a rapidly changing agricultural landscape. This review provides an overview of existing technologies that allow plant biologists to integrate large DNA sequences within a plant host and some associated technical bottlenecks. Additionally, this review explores a selection of emerging techniques in other host systems to inspire tool development in plants.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CsBZR1-CsCEL1 module regulates the susceptibility of cucumber to Meloidogyne incognita by mediating cellulose metabolism","authors":"Tingting Ji,&nbsp;Meiting Liang,&nbsp;Shihui Li,&nbsp;Xingyi Wang,&nbsp;Lujing Cui,&nbsp;Yaqi Bu,&nbsp;Lihong Gao,&nbsp;Si Ma,&nbsp;Yongqiang Tian","doi":"10.1111/tpj.70094","DOIUrl":"https://doi.org/10.1111/tpj.70094","url":null,"abstract":"<div>\u0000 \u0000 <p>Plant-parasitic root knot nematode is a pernicious menace to agriculture. Therefore, uncovering the mechanism of nematode infection is a critical task for crop improvement. Here, with cucumber as material, we found that CsCEL1, encoding β-1,4-endoglucanase to facilitate cellulose degradation, was profoundly induced in the root infected by <i>Meloidogyne incognita</i>. Intriguingly, suppressing the expression of <i>CsCEL1</i> in cucumber conferred resistance to <i>M. incognita</i> infection with reduced activity of β-1,4-endoglucanase but promoted cellulose in the root. Conversely, overexpressing <i>CsCEL1</i> in Arabidopsis increased the number of nematode-induced galls. These results suggest that <i>CsCEL1</i> negatively regulates the resistance to <i>M. incognita</i>. Furthermore, we verified the transcriptional activation of <i>CsCEL1</i> by CsBZR1, a key transcription factor involved in brassinosteroid signaling. Suppressing the expression of <i>CsBZR1</i> in cucumber significantly reduced the size and number of galls and suppressed giant cell formation, with promoted cellulose content. Conversely, overexpressing <i>CsBZR1</i> in Arabidopsis decreased resistance to <i>M. incognita</i>. Exogenous application of brassinosteroid to cucumber suppressed both <i>CsCEL1</i> and <i>CsBZR1</i> expressions, significantly reduced the gall numbers, thus improved resistance to <i>M. incognita</i>. Collectively, these results suggest that the CsBZR1–<i>CsCEL1</i> module is implicated in modulating cellulose content, which may influence <i>M. incognita</i> infection. The finding provides novel insight into the molecular regulations of nematode resistance for breeding resistant varieties or nematode management.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The LbNAM2-LbZDS module enhances drought resistance in wolfberry (Lycium barbarum) by participating in ABA biosynthesis","authors":"Fang Ma,&nbsp;Yunfei Liang,&nbsp;Fanyi Meng,&nbsp;Peizhi Yang,&nbsp;Cong Guo,&nbsp;Hongyan Shi,&nbsp;Mengqiu Ma,&nbsp;Yuqin Wang,&nbsp;Ru Feng,&nbsp;Yiyong Cai,&nbsp;Tixu Hu,&nbsp;Rugang Chen,&nbsp;Yue Yin,&nbsp;Xiangqiang Zhan","doi":"10.1111/tpj.70077","DOIUrl":"https://doi.org/10.1111/tpj.70077","url":null,"abstract":"<div>\u0000 \u0000 <p>Wolfberry (<i>Lycium barbarum</i> L.) fruit, renowned for its high carotenoid content, is extensively used in traditional Chinese herbal medicine and cuisine. Drought is a significant global challenge to crop production, with carotenoids playing crucial roles in enhancing drought resistance in higher plants. <i>ζ</i>-Carotene desaturase (ZDS), a key enzyme in the carotenoid biosynthesis pathway, catalyzes the conversion of <i>ζ</i>-carotene to lycopene. However, the molecular mechanisms by which <i>LbZDS</i> responds to drought stress remain largely unexplored. In this study, we demonstrated that <i>LbZDS</i> transcription is induced by PEG, NaCl, and abscisic acid (ABA) treatments. Overexpression of <i>LbZDS</i> in both wolfberry and tomato plants conferred enhanced drought tolerance by promoting ABA synthesis. We further identified that the NAC transcription factor LbNAM2 directly binds to the promoter region of <i>LbZDS</i> and activates its expression, as evidenced by electrophoretic mobility shift assays, yeast one-hybrid assays, and dual-luciferase assays. Silencing <i>LbNAM2</i>, or dual silencing of <i>LbNAM2</i> and <i>LbZDS</i> via virus-induced gene silencing (VIGS), severely compromised drought tolerance in wolfberry plants. Additionally, overexpression of <i>LbZDS</i> resulted in a marked increase in carotenoid content, while silencing either <i>LbZDS</i>, <i>LbNAM2</i>, or both together led to reduced carotenoid levels. In conclusion, our study provides critical insights into the functional roles and regulatory mechanisms of the LbNAM2–<i>LbZDS</i> module in drought stress response and carotenoid biosynthesis in wolfberry.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INCREASED DNA METHYLATION 3 forms a potential chromatin remodelling complex with HAIRPLUS to regulate DNA methylation and trichome development in tomato","authors":"Tünde Nyikó,&nbsp;Péter Gyula,&nbsp;Szilvia Ráth,&nbsp;Anita Sós-Hegedűs,&nbsp;Tibor Csorba,&nbsp;Syed Hussam Abbas,&nbsp;Károly Bóka,&nbsp;Aladár Pettkó-Szandtner,&nbsp;Ágnes M. Móricz,&nbsp;Béla Péter Molnár,&nbsp;Anna Laura Erdei,&nbsp;György Szittya","doi":"10.1111/tpj.70085","DOIUrl":"https://doi.org/10.1111/tpj.70085","url":null,"abstract":"<p>DNA methylation, a dynamic epigenetic mark influencing gene expression, is regulated by DNA demethylases that remove methylated cytosines at genomic regions marked by the INCREASED DNA METHYLATION (IDM) complex. In <i>Arabidopsis</i>, IDM3, a small α-crystalline domain-containing protein, stabilises the IDM complex. To investigate its role in tomato, we generated <i>slidm3</i> mutants using genome editing. These mutants displayed a ‘hairy’ phenotype with increased glandular trichomes, resembling the <i>hairplus</i> (<i>hap</i>) mutant. Affinity purification of SlIDM3-GFP associated proteins identified several chromatin remodelling factors, including HAP. Genome-wide DNA methylation analysis revealed sequence context dependent alterations in the <i>slidm3-1</i> plants, similar to the <i>hap</i> mutant. CHH methylation was predominantly increased, while CG methylation, particularly in intergenic regions, was decreased in both mutants. This imbalanced methylation suggests the presence of a ‘methylstat’ mechanism attempting to restore methylation levels at abnormally demethylated sites in the mutants. Comparative functional analysis of differentially methylated regions in the <i>slidm3-1</i> and <i>hap</i> mutants identified potential methylation-regulated genes that could be linked to the hairy phenotype. Our findings indicate that SlIDM3 may form a chromatin remodelling complex with HAP, epigenetically regulating trichome development.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural variation in promoters of F3′5′H and ANS correlates with catechins diversification in Thea species of genus Camellia","authors":"Yanrui Zhang,&nbsp;Haiyan Pan,&nbsp;Qiong Wu,&nbsp;Yinong Zha,&nbsp;Jingjing Su,&nbsp;Fangdong Li,&nbsp;Wei Tong,&nbsp;Liang Zhang,&nbsp;Enhua Xia","doi":"10.1111/tpj.70108","DOIUrl":"https://doi.org/10.1111/tpj.70108","url":null,"abstract":"<div>\u0000 \u0000 <p>Catechins were diversely accumulated in <i>Thea</i> plants and were crucial for tea flavor, yet the mechanism underlying the diverse catechins distribution in <i>Thea</i> plants remained elusive. We herein collected a total of 19 <i>Thea</i> and 12 non-<i>Thea</i> plants to investigate their catechins distribution and the underlying mechanism. Results showed that the distribution pattern of catechins in cultivated tea plants significantly differs from that of wild relatives. (+)-Gallocatechin gallate (GCG) was detected in over 50% of wild tea plants but was almost undetectable in cultivated tea plants. Conversely, (−)-Epigallocatechin gallate (EGCG) was extensively distributed in tea cultivars but accumulated extremely low in a few wild relatives such as <i>Camellia tetracocca</i> and <i>C. ptilophylla</i>. Expression analysis found that the expression of <i>flavonoid 3′,5′-hydroxylase</i> (<i>F3′5′H</i>) was highly correlated with EGCG accumulation in <i>Thea</i> plants. Yeast one-hybrid and luciferase assays showed that CsMYB1, a key catechins regulator, could bind to the promoter of <i>F3′5′H</i> and activate its expression to promote EGCG accumulation in cultivated tea plants; yet it was unable to bind to and activate the promoter of <i>F3′5′H</i> of <i>C. tetracocca</i> due to a 14-bp deletion in the promoter, leading to a low content of EGCG. Results also showed that silencing the expression of <i>anthocyanidin synthase</i> (<i>ANS</i>) enhanced the metabolic flux of catechins toward GCG but not EGCG in tea plants, consistent with the observation of high GCG content in <i>C. ptilophylla</i> with low <i>ANS</i> expression. Overall, the results illustrated the mechanism underlying catechins variation in <i>Thea</i> plants and would help to facilitate the utilization of wild tea plants toward future breeding.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyridoxine dehydrogenase SePdx regulates photosynthesis via an association with the phycobilisome in a cyanobacterium","authors":"Shoujin Fan,&nbsp;Wenzhe Li,&nbsp;Zhuo Chen,&nbsp;Zixu Wang,&nbsp;Xiang Cheng,&nbsp;Susu Zhang,&nbsp;Meixue Dai,&nbsp;Jinyu Yang,&nbsp;Leilei Chen,&nbsp;Guoyan Zhao","doi":"10.1111/tpj.70055","DOIUrl":"https://doi.org/10.1111/tpj.70055","url":null,"abstract":"<p>Vitamin B6 (VitB6) deficiency is known to have a deleterious effect on photosynthesis, although the precise mechanism remains unclear. Pyridoxine dehydrogenase is a key protein involved in VitB6 biosynthesis, which facilitates the reversible reduction of pyridoxal (PL) and the oxidation of pyridoxine (PN), thereby contributing to VitB6 production. This study demonstrated the enzymatic activity of a pyridoxine dehydrogenase, SePdx, from the cyanobacterium <i>Synechococcus elongatus</i> PCC 7942 in the oxidation of PN. This protein is localized to the thylakoid membrane, interacts with components of the phycobilisome (PBS) and photosystem I (PSI), and plays a role in general stress responses. Deletion of <i>sepdx</i> leads to a distorted thylakoid membrane, shorter membrane spacing distances, and decreased phycobiliprotein content. Protein–protein interaction studies revealed interactions among SePdx, phycobiliprotein CpcA, and the PSI subunit PsaE. The structural analysis identified key residues that mediate SePdx-CpcA and SePdx-PsaE interactions, which were further confirmed through site-directed mutagenesis. Overall, the findings suggested that SePdx may influence PBS assembly, thereby establishing a link between VitB6 biosynthesis and photosynthesis.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome and metabolome analysis reveals the differential roles of aboveground and belowground pneumatophores in carbon, nitrogen, and sulfur metabolisms in the adaptation of Avicennia marina to coastal intertidal habitat","authors":"Jiajie Zhou,&nbsp;Lihan Zhuang,&nbsp;Yuchen Zhang,&nbsp;Jing Li,&nbsp;Hezi Huang,&nbsp;Saiqi Hao,&nbsp;Dongna Ma,&nbsp;Xiuxiu Wang,&nbsp;Hailei Zheng,&nbsp;Xueyi Zhu","doi":"10.1111/tpj.70092","DOIUrl":"https://doi.org/10.1111/tpj.70092","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Avicennia marina</i> is a dominant mangrove plant that inhabits coastal intertidal wetlands where are featured by low oxygen and nitrogen (N), but high sulfur (S). Its pneumatophore is a unique characteristic of its root system. To understand the roles of the pneumatophore in carbon (C), N, and S metabolisms for intertidal adaptation, we firstly compared the anatomy between aboveground pneumatophore (PA), belowground pneumatophore (PB) and feeding root. The photosynthetic oxygen evolution from PA was assayed by non-invasive micro-test technology, and the metabolisms of C, N, and S between PA and PB were comparatively analyzed by transcriptome and metabolome approaches. The results illustrated that most genes related to photosynthetic C assimilation and S reduction were significantly up-regulated in PA, while genes associated with N uptake, transport, and reduction were significantly up-regulated in PB. Additionally, the expression level of the gene for sulfite oxidase was up-regulated in PA, indicating a tight regulation of S assimilation by PA. Importantly, our findings revealed that key reductases for both S and N assimilation are ferredoxin-dependent, with electrons supplied by the corticular photosynthesis in PA. Integrative multi-omics analysis showed that methylthioadenosine (MTA) was negatively associated with genes related to serine and cysteine biosynthesis but positively connected with genes related to the Yang cycle. This suggests a pivotal role of MTA in coordinating C, N, S, and ethylene metabolism in pneumatophores. The overall results illustrate that the green cortex of PA functions analogously to a leaf, providing reductants and C skeletons for N and S metabolism while coordinating with ethylene metabolism. This facilitates the adaptation of <i>A. marina</i> pneumatophore to the intertidal habitat.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}