The Plant Journal最新文献

{"title":"The making of a leaf tip: how cell division angles define shape","authors":"Martin Balcerowicz","doi":"10.1111/tpj.70453","DOIUrl":"https://doi.org/10.1111/tpj.70453","url":null,"abstract":"<p>Leaves come in an extraordinary range of shapes: they can be simple or dissected, can have smooth or toothed margins and can display a variety of lobes, points and indentations. These shape variations have important functional consequences, affecting light capture, water loss, wind resistance and even herbivore interactions (Chitwood & Sinha, <span>2016</span>). Leaves originate as small primordia on the flanks of the shoot apical meristem, from which they grow and expand until they reach their mature form. The rate, duration and spatial patterning of cell division and expansion are key determinants of leaf size and shape. These processes are finely tuned by complex gene regulatory networks that respond to both internal and external signals (Schneider et al., <span>2024</span>).</p><p>Hirokazu Tsukaya has long studied the mechanisms underpinning leaf development. Starting with the simple ovate leaves of the model plant <i>Arabidopsis thaliana</i>, his group now explores leaf shape from an evo-devo perspective, in a wide range of model and non-model species. Among these is <i>Triadica sebifera</i>, the Chinese tallow tree – a member of the spurge family and an oil-producing species of economic significance. Its leaves are distinctly shaped, featuring a rounded base and a sharply tapering (acuminate) tip, linked by concave joint regions (Figure 1a). This shape can be quantified by its curvature, that is, how much the contour deviates from a straight line (Figure 1b): the base shows moderately positive curvature (convex, rounded), the joint regions display negative curvature (concave), and the tip reaches a maximum curvature with a sharp point. Until now, it has remained unknown how this distinctive apex forms.</p><p>Zining Wang, a Ph.D. student in Tsukaya's lab and first author of the highlighted study, combines mathematical modelling with developmental biology to investigate leaf shape formation. Surprised by the lack of research into acuminate tip formation, Wang took on the challenge of dissecting leaf shape formation in <i>T. sebifera</i> – made more complex by it being a woody, non-model species that required significant effort to establish reliable germination and growth protocols. Wang began by describing leaf growth using contour growth mapping, an approach that represents the leaf outline as a series of contour points. Growth is simulated by moving these points outward at defined speeds. When growth was isotropic (equal in all directions), the model produced a circular leaf. Vertical anisotropic growth yielded an elliptical form. However, combining isotropic growth at the base with vertical anisotropic growth at the tip generated a shape resembling <i>T. sebifera</i> leaves, indicating that distinct regional growth patterns are required.</p><p>To assess whether this biregional growth is reflected at the cellular level, Wang and colleagues analysed cell shapes and division patterns during leaf development. Interestingly, the sharp ap","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70453","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144914983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene editing unlocks superior mutants from once detrimental RFL for enhanced rice yield traits","authors":"Jiajun Liu,&nbsp;Ye Song,&nbsp;Min Mei,&nbsp;Xuebin Zhao,&nbsp;Shu Wan,&nbsp;Qian Xun,&nbsp;Yayi Meng,&nbsp;Jianyu An,&nbsp;Ganghua Li,&nbsp;Yanfeng Ding,&nbsp;Chengqiang Ding","doi":"10.1111/tpj.70454","DOIUrl":"https://doi.org/10.1111/tpj.70454","url":null,"abstract":"<div>\u0000 \u0000 <p><i>RICE FLORICULA LEAFY/ABERRANT PANICLE ORGANIZATION 2</i> (<i>RFL/APO2</i>) is a master regulator of panicle morphogenesis and development in rice. Traditionally, mutations in <i>RFL</i> have led to severe growth phenotypes and decreased rice yield, labeling it as detrimental. However, the present study challenged this perception by utilizing CRISPR/Cpf1 and single-base gene-editing technologies to generate a series of site-directed <i>rfl</i> mutants. Our findings revealed that the evolutionarily conserved sterile alpha motif (SAM) domain and DNA-binding domain (DBD), as well as the intron region of <i>RFL</i>, all play roles in regulating rice morphological development and yield traits. Specifically, introns and the SAM domain are primarily involved in panicle development, whereas the DBD and its key functional sites are closely associated with morphological development and yield. Notably, the amino acid at position 266 was found to be a critical site for RFL regulation of grain shape, significantly affecting grain weight, with changes in the expression levels of genes involved in grain length and panicle weight regulation, such as <i>GRF1</i> and <i>SPL16</i>. This study not only expands our understanding of the role of RFL in monocot plants but also provides a novel perspective on how gene editing can transform a gene once considered detrimental to improve yield traits in cereal crops. These findings suggest that the number of genes available for optimizing rice phenotypes through gene editing can be significantly increased.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsMATE7-mediated flavonol accumulation regulates pollen tube growth in rice","authors":"Shijun Fan,&nbsp;Kailai Huang,&nbsp;Yan Yang,&nbsp;Yi Xi,&nbsp;Huan Ye,&nbsp;Lei Xu,&nbsp;Weilan Chen,&nbsp;Peng Gao,&nbsp;Ting Li,&nbsp;Bin Tu,&nbsp;Hua Yuan,&nbsp;Bingtian Ma,&nbsp;Yuping Wang,&nbsp;Zhaohui Zhong,&nbsp;Jiawei Xiong,&nbsp;Liangzhu Kang,&nbsp;Shiwen Tang,&nbsp;Xuewei Chen,&nbsp;Chengbin Xiang,&nbsp;Shigui Li,&nbsp;Peng Qin","doi":"10.1111/tpj.70449","DOIUrl":"https://doi.org/10.1111/tpj.70449","url":null,"abstract":"<div>\u0000 \u0000 <p>Flavonols have been implicated in male sterility and pollen tube growth for over three decades; however, the molecular mechanisms mediating their accumulation in pollen grains remain poorly understood. In this study, a multidrug and toxic compound extrusion (MATE) transporter, OsMATE7, was identified as a key regulator of flavonol accumulation in mature pollen grains, thereby promoting pollen tube growth in rice (<i>Oryza sativa</i>). Mutation of <i>OsMATE7</i> resulted in a significant reduction in seed setting rates. Reciprocal crosses between <i>OsMATE7</i> knockout (KO) and wild-type plants, along with both <i>in vivo</i> and <i>in vitro</i> germination assays, demonstrated that the mutant exhibited male sterility due to defective pollen tube growth. OsMATE7 was found to be specifically expressed in pollen and localized to the endoplasmic reticulum (ER). Diphenylboric acid 2-aminoethylester (DPBA) staining assay revealed a significant reduction in flavonol accumulation in mature pollen grains of <i>OsMATE7</i>. Functional analysis of flavonol transport and flavonoid content confirmed that OsMATE7 serves as a potential flavonol transporter, facilitating the accumulation of flavonols in mature pollen grains. Further investigation revealed that flavonol deficiency in mutant pollen grains inhibited α-amylase activity and blocked starch hydrolysis, ultimately impairing pollen tube growth. These findings elucidate a novel mechanism by which OsMATE7 regulates flavonol distribution to control pollen tube growth, providing new insights into the role of MATE proteins in seed setting and crop breeding.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restoring cytonuclear harmony: Distinct strategies in Arabidopsis auto- and allopolyploids","authors":"Mehrdad Shahbazi,&nbsp;Jana Kneřová,&nbsp;Denisa Kubíková,&nbsp;Alžběta Doležalová,&nbsp;Marek Szecówka,&nbsp;Yasmim Dutra Santos,&nbsp;Jonathan F. Wendel,&nbsp;Joel Sharbrough,&nbsp;David Kopecký","doi":"10.1111/tpj.70451","DOIUrl":"https://doi.org/10.1111/tpj.70451","url":null,"abstract":"<p>Plants rely on tight coordination between nuclear, mitochondrial, and chloroplast genomes to form essential multi-enzyme cytonuclear complexes. Whole-genome duplication (WGD) doubles the nuclear genome, potentially disrupting cytonuclear stoichiometry unless organellar genomes respond accordingly. Targeted analyses of chloroplasts and mitochondria enabled us to dissect the extent and mechanisms of adjustments in both organelles immediately after WGD and across generations in Arabidopsis auto- and allopolyploids. We observed a substantial overcompensation of organellar genome copies in both organelles in early-generation autotetraploids primarily through multiplication of DNA copies within organelles rather than increasing the number of organelles. Despite higher DNA content, mitochondria maintained their volume, and chloroplasts were even smaller. In successive generations, chloroplast DNA copy numbers continued to rise, whereas mitochondrial DNA copies declined. Gene expression patterns also differed between chloroplasts and mitochondria and between auto- and allopolyploids. In autopolyploids, immediate transcriptional changes were minimal, but by the fourth generation after WGD, nuclear genes involved in mitochondria-nuclear complexes were downregulated. In allopolyploids, transcriptional changes appeared immediately in the first generation (chloroplast genes were upregulated and mitochondrial genes were downregulated). Our findings demonstrate that cytonuclear balance is restored through dynamic, organelle-specific, and polyploid-type-specific mechanisms. These insights advance our understanding of the evolution of polyploid genomes.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70451","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144910154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorophyll b is essential for the growth, photoprotection, and photosystem I assembly in wheat","authors":"Hao-Tian Mao,&nbsp;Xuan Pang,&nbsp;Teng Li,&nbsp;Ying Qin,&nbsp;Zhong-Wei Zhang,&nbsp;Shu Yuan,&nbsp;Ming Yuan,&nbsp;Marian Brestic,&nbsp;Yang-Er Chen","doi":"10.1111/tpj.70442","DOIUrl":"https://doi.org/10.1111/tpj.70442","url":null,"abstract":"<div>\u0000 \u0000 <p>Chlorophyll (Chl) <i>b</i> deficiency leads to vulnerability to high light and oxidative stress in wheat plants, while the detailed mechanism by which Chl <i>b</i> is involved in photoprotection remains unclear in plants. In this study, the roles of thylakoid protein composition and complexes in photosynthetic electron transport, photoprotective responses, and energy dissipation were investigated in Chl <i>b</i>-deficient mutant lines (ANK-32A) and the wild type (WT) of wheat. Compared to the WT, ANK-32A showed higher non-photochemical quenching (NPQ), slower state transitions, and a significant decline in the amount of Lhca1–4, Lhcb1–3, and PSII-LHCII supercomplexes at the early growth stage. Because of the low Chl <i>b</i> content, ANK-32A also exhibited a low PSI/PSII ratio in the first leaf (the youngest leaf) compared to the WT. In late growth stages, the amounts of Lhcb2, Lhcb3, PSI proteins (Lhca1–4), PSII-LHCII supercomplexes, and PSI and PSII dimers were still lower than in the WT. Immunoblotting analysis and protein mass spectrometry indicated that ANK-32A possessed a high PSI assembly intermediate (PSI*) content relative to the WT. In addition, field experiments further demonstrated that the low Chl content and the PSI efficiency in the flag leaf as well as low yield were observed in ANK-32A compared to the WT. Taken together, this study reveals that chlorophyll <i>b</i> deficiency in wheat alters the organization of thylakoid proteins, which in turn leads to disrupted assembly of PSI complexes, increases PSI photoinhibition, and eventually reduces the photoprotective capacity.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of FLOWERING LOCUS T-related genes and their putative gene regulatory network in semi-winter Brassica napus cultivar Zhongshaung11","authors":"Juanjuan Wang,&nbsp;Hao-Ran Zhou,&nbsp;Petra Tänzler,&nbsp;Na Ding,&nbsp;Jing Wang,&nbsp;Franziska Turck","doi":"10.1111/tpj.70443","DOIUrl":"https://doi.org/10.1111/tpj.70443","url":null,"abstract":"<p>In many species, <i>FLOWERING LOCUS T</i> (<i>FT</i>)-like genes promote the floral transition by integrating environmental signals, in particular photoperiod, and internal cues. Here we show that <i>Brassica napus</i> contains six <i>FT</i>-like genes and two pseudogenes belonging to three orthogroups. All <i>B. napus FT</i>-like genes induce early flowering when expressed at the shoot apical meristems of <i>Arabidopsis thaliana ft</i> mutants; however, <i>BnaFT.C6</i> and non-orthologous <i>FT</i>-like genes do not encode fully functional mobile florigens. In the case of <i>BnFT.C6</i>, the functional change is associated with a T to C amino acid change that is restricted to semi-winter accessions. Expression of orthologs of <i>FT</i> is photoperiod-dependent, and two distal enhancers are conserved; however, the homeologs <i>BnaFT.A7</i> and <i>BnaFT.C6</i> show rearrangements of DNA motifs binding NF-Y/CO and NF-Y transcriptional activator complexes between the promoter and downstream enhancers. Motif rearrangements correlate with differences in tissue-specific expression. Furthermore, homeologs with rearranged motifs could not be transactivated by <i>B. napus</i> CO in transient assays, although they show LD photoperiod-dependent expression. We propose that differential diurnal expression of <i>NF-Y</i> genes contributes to the photoperiod-dependent regulation of <i>B. napus FT</i> genes.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consequences of interspecific plant hybridization on metabolic diversity in naturally occurring hybrid swarms","authors":"Olga Zafra-Delgado,&nbsp;Fabian Schneider,&nbsp;Yoko Nakamura,&nbsp;Michael Reichelt,&nbsp;Jonathan Gershenzon,&nbsp;Frank H. Hellwig,&nbsp;Tobias G. Köllner","doi":"10.1111/tpj.70444","DOIUrl":"https://doi.org/10.1111/tpj.70444","url":null,"abstract":"<p>Interspecific hybridization has influenced plant evolution and diversification. However, how hybridization may affect metabolic diversity, especially in naturally occurring hybridization zones, is unclear. In this study, we selected a <i>Baccharis</i> (Asteraceae) hybrid complex consisting of <i>B. linearis</i>, <i>B</i>. <i>macraei</i>, and <i>B. × intermedia</i> and characterized its metabolic profiles in multiple hybridization zones in central Chile to determine how hybridization affects plant chemistry. Untargeted liquid chromatography–time of flight mass spectrometry analysis of a total of 411 plant individuals collected in the field revealed that the hybrid <i>B. × intermedia</i> combines the metabolic profiles of its two parental species, <i>B. linearis</i> and <i>B</i>. <i>macraei</i>, independent of season, location, and environment. This combinatorial effect was observed in the specialized metabolites, while the primary metabolism did not differ between species. The metabolic diversity of the hybrid exceeded that of the parental species and was influenced by latitude, with higher metabolic diversity in the northern populations than in those in the south. In summary, our results demonstrate that natural interspecific hybridization can quickly increase the diversity of specialized metabolites. This could enhance protection against biotic or abiotic stressors, particularly in changing environmental conditions.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70444","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A GDSL lipase confers resistance to piercing-sucking insects in tobacco by strengthening leaf cuticle","authors":"Na Chen,&nbsp;Yu-Die Xiong,&nbsp;Chi Zou,&nbsp;Yu-Wei Zhong,&nbsp;Hui Du,&nbsp;Yu-Jie Chi,&nbsp;Chan Zhao,&nbsp;Shu-Sheng Liu,&nbsp;Xiao-Wei Wang","doi":"10.1111/tpj.70440","DOIUrl":"https://doi.org/10.1111/tpj.70440","url":null,"abstract":"<div>\u0000 \u0000 <p>Piercing-sucking insects, such as whiteflies and aphids, cause massive economic losses in major crops around the world. During feeding, the stylets of piercing-sucking insects navigate cuticles, cell walls, epidermal cells, and mesophyll cells; thus, these barriers are vital for the resistance of plants to insects. However, the relationship between insect stylet probing behavior and the composition and structure of these barriers remains unclear. Here, we identified a tobacco <i>Cuticle Related Factor</i> (<i>NtCRF</i>), which was induced significantly by whitefly infestation. Bioassays showed that <i>NtCRF</i> positively regulated plant resistance against whiteflies and green peach aphids. Silencing of <i>NtCRF</i> did not affect plant jasmonic acid (JA) and salicylic acid (SA) defenses but shortened the stylet probing time of phloem-feeders. Further studies confirmed that silencing of <i>NtCRF</i> resulted in significant structure destruction of the leaf cuticle and led to increased epidermal permeability. Overexpression of <i>NtCRF</i> in Arabidopsis also significantly enhanced the plant's resistance against whiteflies and green peach aphids. Our findings expand understanding of plant–insect interactions and provide a strategy for genetic improvement of crop resistance against piercing-sucking insects.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144897396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoselenium application improves post-harvest fruit quality and disease resistance in tomato","authors":"Xiaoqing He,&nbsp;Shiyu Ying,&nbsp;Yi Xu,&nbsp;Zhuo Gao,&nbsp;Yi Wu,&nbsp;Mingchun Liu,&nbsp;Mengbo Wu","doi":"10.1111/tpj.70432","DOIUrl":"https://doi.org/10.1111/tpj.70432","url":null,"abstract":"<p>Fruits constitute a vital component of a nutritious diet but are highly perishable, contributing substantially to food waste. Consequently, identifying safe and edible biological agents to enhance product quality and extend shelf life is of critical importance. In this study, we demonstrate that exogenous pre-harvest foliar application with nanoselenium (nano-Se) in tomato enhances fruit quality, prolongs fruit shelf life, and enhances the resistance of tomato fruit to <i>Botrytis cinerea</i> infection. Transcriptomic analysis revealed coordinated upregulation of genes associated with quality maintenance and modulation of phytohormone-related pathways. Notably, nano-Se treatment induced expression patterns of ripening-related genes that resembled those triggered by ethylene (ET) but were antagonistic to the effects of 1-MCP. We further demonstrated that although <i>SlMYC2</i> knockout increased susceptibility to <i>B. cinerea</i>, nano-Se application restored resistance in a manner independent of the SlMYC2-associated jasmonic acid signaling pathway, implicating ET as the primary regulatory mechanism. Collectively, these findings support nano-Se as a promising biostimulant for reducing post-harvest losses while preserving the nutritional and sensory quality of tomato fruits.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70432","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABERRANT CARBOHYDRATE PARTITIONING 1 modulates sucrose allocation by regulating cell wall formation in rice","authors":"Wenqiang Shen,&nbsp;Zan Xiao,&nbsp;Ziyu Xie,&nbsp;Cheng Qin,&nbsp;Xiaoyan Zhu,&nbsp;Ting Zhang,&nbsp;Nan Wang,&nbsp;Yunfeng Li,&nbsp;Xianchun Sang,&nbsp;Yinghua Ling,&nbsp;Guanghua He","doi":"10.1111/tpj.70430","DOIUrl":"https://doi.org/10.1111/tpj.70430","url":null,"abstract":"<div>\u0000 \u0000 <p>Sucrose (Suc) is transported from source leaves to sink tissues to sustain plant growth, development, and crop yield. However, the molecular mechanisms underlying carbohydrate partitioning still remain largely unclear. Here, we report a rice (<i>Oryza sativa</i>) mutant <i>aberrant carbohydrate partitioning 1</i> (<i>acp1</i>), which hyperaccumulates carbohydrates in leaves and exhibits leaf chlorosis and premature senescence. <i>ACP1</i> encodes a novel protein that contains two conserved domains of unknown function, DUF4220 and DUF594. Subcellular localization in rice and tobacco showed that ACP1 was localized in the endoplasmic reticulum. In situ expression analysis showed that <i>ACP1</i> was mainly expressed in vascular bundles. Dye and sugar export experiments suggested that sugar trafficking through vascular tissues was impaired in the <i>acp1</i> mutant. The <i>acp1</i> mutant exhibits a significant cellulose deficiency in its leaves. Transmission electron microscopy experiments found that the abnormal cell wall ultrastructure in <i>acp1</i>. Furthermore, turgor pressure in source leaves of <i>acp1</i> decreased compared with WT. Together, these results suggest that <i>ACP1</i> plays a critical role in the partitioning of carbohydrates by regulating cell wall formation, which in turn affects the overall carbohydrate distribution and plant physiology.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}