The Plant Journal最新文献

{"title":"Synthetic minichromosomes in plants: past, present, and promise","authors":"James A. Birchler,&nbsp;Jacob Kelly,&nbsp;Jasnoor Singh,&nbsp;Hua Liu,&nbsp;Zhengzhi Zhang,&nbsp;Si Nian Char,&nbsp;Malika Sharma,&nbsp;Hua Yang,&nbsp;Patrice S. Albert,&nbsp;Bing Yang","doi":"10.1111/tpj.17142","DOIUrl":"10.1111/tpj.17142","url":null,"abstract":"<div>\u0000 \u0000 <p>The status of engineered mini-chromosomes/artificial chromosomes/synthetic chromosomes in plants is summarized. Their promise is that they provide a means to accumulate foreign genes on an independent entity other than the normal chromosomes, which would facilitate stacking of novel traits in a way that would not be linked to endogenous genes and that would facilitate transfer between lines. Centromeres in plants are epigenetic, and therefore the isolation of DNA underlying centromeres and reintroduction into plant cells will not establish a functional kinetochore, which obviates this approach for <i>in vitro</i> assembly of plant artificial chromosomes. This issue was bypassed by using telomere-mediated chromosomal truncation to produce mini-chromosomes with little more than an endogenous centromere that could in turn be used as a foundation to build synthetic chromosomes. Site-specific recombinases and various iterations of CRISPR-Cas9 editing provide many tools for the development and re-engineering of synthetic chromosomes.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2356-2366"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defense-related callose synthase PMR4 promotes root hair callose deposition and adaptation to phosphate deficiency in Arabidopsis thaliana","authors":"Kentaro Okada,&nbsp;Koei Yachi,&nbsp;Tan Anh Nhi Nguyen,&nbsp;Satomi Kanno,&nbsp;Shigetaka Yasuda,&nbsp;Haruna Tadai,&nbsp;Chika Tateda,&nbsp;Tae-Hong Lee,&nbsp;Uyen Nguyen,&nbsp;Kanako Inoue,&nbsp;Natsuki Tsuchida,&nbsp;Taiga Ishihara,&nbsp;Shunsuke Miyashima,&nbsp;Kei Hiruma,&nbsp;Kyoko Miwa,&nbsp;Takaki Maekawa,&nbsp;Michitaka Notaguchi,&nbsp;Yusuke Saijo","doi":"10.1111/tpj.17134","DOIUrl":"10.1111/tpj.17134","url":null,"abstract":"<p>Plants acquire phosphorus (P) primarily as inorganic phosphate (Pi) from the soil. Under Pi deficiency, plants induce an array of physiological and morphological responses, termed phosphate starvation response (PSR), thereby increasing Pi acquisition and use efficiency. However, the mechanisms by which plants adapt to Pi deficiency remain to be elucidated. Here, we report that deposition of a β-1,3-glucan polymer called callose is induced in <i>Arabidopsis thaliana</i> root hairs under Pi deficiency, in a manner independent of PSR-regulating <i>PHR1/PHL1</i> transcription factors and <i>LPR1/LPR2</i> ferroxidases. Genetic studies revealed <i>PMR4</i> (<i>GSL5</i>) callose synthase being required for the callose deposition in Pi-depleted root hairs. Loss of <i>PMR4</i> also reduces Pi acquisition in shoots and plant growth under low Pi conditions. The defects are not recovered by simultaneous disruption of <i>SID2</i>, mediating defense-associated salicylic acid (SA) biosynthesis, excluding SA defense activation from the cause of the observed <i>pmr4</i> phenotypes. Grafting experiments and characterization of plants expressing <i>PMR4</i> specifically in root hair cells suggest that a PMR4 pool in the cell type contributes to shoot growth under Pi deficiency. Our findings thus suggest an important role for <i>PMR4</i> in plant adaptation to Pi deficiency.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2639-2655"},"PeriodicalIF":6.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17134","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytogenetic characterization of EPSPS gene amplification in glyphosate-resistant Hordeum glaucum and Bromus diandrus from Australia","authors":"Md Mazharul Islam,&nbsp;Bikram S. Gill,&nbsp;Jenna M. Malone,&nbsp;Christopher Preston,&nbsp;Mithila Jugulam","doi":"10.1111/tpj.17128","DOIUrl":"10.1111/tpj.17128","url":null,"abstract":"<div>\u0000 \u0000 <p>As a result of extensive selection, two polyploid grass weeds, <i>Hordeum glaucum</i> (northern barley grass; 2<i>n</i> = 4<i>x</i> = 28) and <i>Bromus diandrus</i> (ripgut brome; 2<i>n</i> = 8<i>x</i> = 56), have evolved resistance to glyphosate, in Australia. Previous research suggested amplification of 5-enolpyruvylshikimate-3-Phosphate synthase (<i>EPSPS</i>) gene confers resistance in these two weed species. The objective of this research was to investigate the genomic organization of the <i>EPSPS</i> gene in these two species through molecular cytogenetic analyses of fluorescence <i>in situ</i> hybridization (FISH) to understand possible mechanism of amplification of this gene. <i>EPSPS</i> copy number of <i>H. glaucum</i> and <i>B. diandrus</i> plants was estimated via quantitative polymerase chain reaction. The susceptible plants of both species had one copy of <i>EPSPS</i>, whereas the resistant plants of <i>H. glaucum</i> and <i>B. diandrus</i> had 14–17 and 16–32 copies, respectively. FISH analysis of glyphosate-susceptible (Hg-RWS) <i>H. glaucum</i>, revealed four faint signals of the <i>EPSPS</i> gene in two pairs of homologous chromosomes, at the telomeric region. The glyphosate-resistant <i>H. glaucum</i> (Hg-YP1) also showed amplification of <i>EPSPS</i> gene at telomeric regions in two pairs of homologous chromosomes, but the signals were brighter and appeared as cluster of <i>EPSPS</i> genes. Similarly, the glyphosate-susceptible <i>B. diandrus</i> (Bd-S) plants showed faint signals of <i>EPSPS</i> gene on two homologous chromosomes, at the telomeric position. However, samples of two glyphosate-resistant, <i>B. diandrus</i>, Bd-SA988 and Bd-Vic showed much brighter hybridization signals of <i>EPSPS</i> gene, located at the telomere on two homologous chromosomes, suggesting an increase in <i>EPSPS</i> gene copies at this position. Overall, unequal crossover during meiosis may have triggered the initial <i>EPSPS</i> gene duplication sparking the evolution of glyphosate resistance.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2553-2562"},"PeriodicalIF":6.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutation of rice EARLY LEAF LESION AND SENESCENCE 1 (ELS1), which encodes an anthranilate synthase α-subunit, induces ROS accumulation and cell death through activating the tryptophan synthesis pathway in rice","authors":"Wenhao Li,&nbsp;Weimin Cheng,&nbsp;Hongrui Jiang,&nbsp;Cheng Fang,&nbsp;Lingling Peng,&nbsp;Liangzhi Tao,&nbsp;Yue Zhan,&nbsp;Xianzhong Huang,&nbsp;Bojun Ma,&nbsp;Xifeng Chen,&nbsp;Yuejin Wu,&nbsp;Binmei Liu,&nbsp;Xiangdong Fu,&nbsp;Kun Wu,&nbsp;Yafeng Ye","doi":"10.1111/tpj.17141","DOIUrl":"10.1111/tpj.17141","url":null,"abstract":"<div>\u0000 \u0000 <p>Lesion-mimic mutants (LMMs) serve as valuable resources for uncovering the molecular mechanisms that govern programmed cell death (PCD) in plants. Despite extensive research, the regulatory mechanisms of PCD and lesion formation in various LMMs remain to be fully elucidated. In this study, we identified a rice LMM named <i>early leaf lesion and senescence 1</i> (<i>els1</i>), cloned the causal gene through map-based cloning, and confirmed its function through complementation. <i>ELS1</i> encodes an anthranilate synthase α-subunit involved in anthranilate biosynthesis. It is predominantly localized in chloroplasts and is primarily expressed in light-exposed tissues. Mutation of <i>ELS1</i> triggers upregulation of its homologous gene, <i>ASA1</i>, via a genetic compensation response, leading to the activation of the tryptophan (Trp) synthesis pathway and amino acid metabolism. The accumulation of abnormal Trp-derived intermediate metabolites results in reactive oxygen species (ROS) production and abnormal PCD in the <i>els1</i> mutant, ultimately causing the leaf lesion phenotype. The <i>els1</i> mutant also exhibits reduced chlorophyll content, upregulation of genes related to chloroplast degradation and leaf senescence, and decreased activity of photosynthetic proteins, indicating that <i>ELS1</i> plays a role in chloroplast development. These factors collectively contribute to the premature leaf senescence observed in the <i>els1</i> mutant. Our findings shed light on the role of <i>ELS1</i> in regulating ROS accumulation and PCD in rice, providing further genetic insights into the molecular mechanisms governing leaf lesions and senescence.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2723-2737"},"PeriodicalIF":6.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global impacts of peroxisome and pexophagy dysfunction revealed through multi-omics analyses of lon2 and atg2 mutants","authors":"DurreShahwar Muhammad,&nbsp;Natalie M. Clark,&nbsp;Nathan E. Tharp,&nbsp;Elizabeth C. Chatt,&nbsp;Richard D. Vierstra,&nbsp;Bonnie Bartel","doi":"10.1111/tpj.17129","DOIUrl":"10.1111/tpj.17129","url":null,"abstract":"<p>Peroxisomes house diverse metabolic pathways that are essential for plant and animal survival, including enzymes that produce or inactivate toxic byproducts. Despite the importance of peroxisomes and their collaborations with other organelles, the mechanisms that trigger or prevent peroxisome turnover and the cellular impacts of impaired peroxisomes are incompletely understood. When <i>Arabidopsis thaliana</i> LON2, a peroxisomal protein with chaperone and protease capacity, is disrupted, metabolic dysfunction and protein instability in peroxisomes ensue. Paradoxically, preventing autophagy in <i>lon2</i> mutants appears to normalize peroxisomal metabolism and stabilize peroxisomal proteins—hinting at a role for autophagy in causing the peroxisomal defects observed in <i>lon2</i> seedlings. Using a combination of transcriptomics, proteomics, and <i>in silico</i> investigations, we compared wild type to <i>lon2</i> and autophagy null mutants and double mutants. Through this analysis, we found that impeding autophagy via an <i>atg2</i> null mutation alleviated several of the global defects observed when LON2 is absent. Moreover, we revealed processes influenced by LON2 that are independent of autophagy, including impacts on lipid droplet and chloroplast protein levels. Finally, we identified and classified potential LON2 substrates, which include proteins that might provide signal(s) for pexophagy.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2563-2583"},"PeriodicalIF":6.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perception and processing of stress signals by plant mitochondria","authors":"Jennifer Selinski,&nbsp;Stephanie Frings,&nbsp;Romy Schmidt-Schippers","doi":"10.1111/tpj.17133","DOIUrl":"10.1111/tpj.17133","url":null,"abstract":"<p>In the course of their life, plants continuously experience a wide range of unfavourable environmental conditions in the form of biotic and abiotic stress factors. The perception of stress via various organelles and rapid, tailored cellular responses are essential for the establishment of plant stress resilience. Mitochondria as the biosynthetic sites of energy equivalents in the form of ATP—provided in order to enable a multitude of biological processes in the cell—are often directly impacted by external stress factors. At the same time, mitochondrial function may fluctuate to a tolerable extent without the need to activate downstream retrograde signalling cascades for stress adaptation. In this Focus Review, we summarise the current state of knowledge on the perception and processing of stress signals by mitochondria and show which layers of retrograde signalling, that is, those involving transcription factors, metabolites, but also enzymes with moonlighting functions, enable communication with the nucleus. Also, light is shed on signal integration between mitochondria and chloroplasts as part of retrograde signalling. With this Focus Review, we aim to show ways in which organelle-specific communication can be further researched and the collected data used in the long-term to strengthen plant resilience in the context of climate change.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2337-2355"},"PeriodicalIF":6.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The homeostasis of AtMYB4 is maintained by ARA4, HY5, and CAM7 during Arabidopsis seedling development","authors":"Siddhartha Dutta,&nbsp;Riya Basu,&nbsp;Abhideep Pal,&nbsp;M. H. Kunalika,&nbsp;Sudip Chattopadhyay","doi":"10.1111/tpj.17126","DOIUrl":"10.1111/tpj.17126","url":null,"abstract":"<div>\u0000 \u0000 <p>Calmodulin7 (CAM7) is a key transcription factor of Arabidopsis seedling development. CAM7 works together with HY5 bZIP protein to promote photomorphogenesis at various wavelengths of light. In this study, we show that AtMYB4, identified from a yeast two-hybrid screen, physically interacts with CAM7 and works as a positive regulator of photomorphogenesis at various wavelengths of light. CAM7 and HY5 directly bind to the promoter of <i>AtMYB4</i> to promote its expression for photomorphogenic growth. On the other hand, ARA4, identified from the same yeast two-hybrid screen, works as a negative regulator of photomorphogenic growth specifically in white light. The double mutant analysis reveals that the altered hypocotyl elongation of <i>atmyb4</i> and <i>ara4</i> is either partly or completely suppressed by additional loss of function of <i>CAM7</i>. Furthermore, <i>ARA4</i> genetically interacts with <i>AtMYB4</i> in an antagonistic manner to suppress the elongated hypocotyl phenotype of <i>atmyb4</i>. The transactivation studies reveal that while CAM7 activates the promoter of <i>AtMYB4</i> in association with HY5, ARA4 negatively regulates <i>AtMYB4</i> expression. Taken together, these results demonstrate that working as a negative regulator of photomorphogenesis, ARA4 plays a balancing act on CAM7 and HY5-mediated regulation of <i>AtMYB4</i>.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2515-2535"},"PeriodicalIF":6.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRX h2–PP2AC2 module serves as a convergence node for aluminum stress and leaf senescence signals, regulating cell death via ABA-mediated ROS pathway","authors":"Xia Li,&nbsp;Guijun Su,&nbsp;Chunliu Pan,&nbsp;Jie Zhan,&nbsp;Aiqin Wang,&nbsp;Zhuqiang Han,&nbsp;Dong Xiao,&nbsp;Longfei He","doi":"10.1111/tpj.17131","DOIUrl":"10.1111/tpj.17131","url":null,"abstract":"<div>\u0000 \u0000 <p>ROS/redox signaling plays an important role in the regulation of signal transduction and acclimation pathways activated by multiple abiotic stresses and leaf senescence. However, the regulatory events that produce ROS under different stimuli are far from clear. Here, we report the elucidation of the molecular mechanism of an h type thioredoxin, AhTRX h2, positively regulates Al sensitivity and leaf senescence by promoting ROS. <i>AhTRX h2</i> transcript levels increased greatly during both natural senescence and Al stress condition in peanut. Ectopic expression of <i>AhTRX h2</i> in Arabidopsis conferred Al sensitivity as well as premature leaf senescence, manifested by multiple indices, including inhibiting root elongation, severe cell death, and accelerated expression of <i>MC1</i> and <i>CEX17</i>. AhTRX h2 exhibited similar functions to AtTRX h2, as <i>AhTRX h2</i> was able to restore the phenotypes of the <i>AtTRX h2</i> defective mutant (<i>trxh2-4</i>) which showed Al tolerant and late senescence phenotypes. The knock down of <i>AhTRX h</i>2 markedly suppressed Al- and senescence-induced cell death in peanut. AhTRX h2 could recruit catalytic subunit of protein phosphatase 2A (PP2AC2) to form a stable complex. The interaction between AhTRX h2 and AtPP2AC2, as well as AhPP2AC2 and AtTRX h2 was also proved. Overexpression of <i>AhPP2AC2</i> significantly enhanced Al sensitivity and leaf senescence in Arabidopsis. Protein stability assay revealed that AhTRX h2 was more stable during aging or aluminum stress. Moreover, PP2AC2 could greatly enhance the stability of AhTRX h2 <i>in vivo</i>. Consistent with these observations, overexpression of <i>AhPP2AC2</i> effectively enhanced <i>AhTRX h2</i>-induced Al sensitivity and precocious leaf senescence. AhTRX h2 and AhPP2AC2 required ABA and ROS in response to cell death under Al stress and senescence, and it was evidence to suggest that ABA acted upstream of ROS in this process. Together, AhTRX h2 and AhPP2AC2 constitute a stable complex that promotes the accumulation of ABA and ROS, effectively regulate cell death. These findings suggest that TRX h2-PP2AC2-mediated pathway may be a widespread mechanism in regulating Al stress and leaf senescence.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2602-2622"},"PeriodicalIF":6.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient sorghum and maize transformation using a ternary vector system combined with morphogenic regulators","authors":"Juan B. Fontanet-Manzaneque,&nbsp;Jari Haeghebaert,&nbsp;Stijn Aesaert,&nbsp;Griet Coussens,&nbsp;Laurens Pauwels,&nbsp;Ana I. Caño-Delgado","doi":"10.1111/tpj.17101","DOIUrl":"10.1111/tpj.17101","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Sorghum bicolor</i> (sorghum) is a vital C4 monocotyledon crop cultivated in arid regions worldwide, valued for its significance in both human and animal nutrition. Despite its agricultural prominence, sorghum research has been hindered by low transformation frequency. In this study, we examined sorghum transformation using the pVS1-VIR2 ternary vector system for <i>Agrobacterium</i>, combined with the morphogenic genes <i>BABY BOOM</i> and <i>WUSCHEL2</i> and selection using G418. We optimized <i>Agrobacterium</i>-mediated infection, targeting key parameters such as bacterial optical density, co-cultivation time, and temperature. Additionally, an excision-based transformation system enabled us to generate transgenic plants free of morphogenic regulators. The method yielded remarkable transformation frequencies, reaching up to 164.8% based on total isolated plantlets. The same combination of ternary vector, morphogenic genes and geneticin-based selection also resulted in a marked increase in transformation efficiency of the <i>Zea mays</i> (maize) inbred line B104. The potential for genomic editing using this approach positions it as a valuable tool for the development of sorghum and maize varieties that comply with evolving European regulations. Our work marks a significant stride in sorghum biotechnology and holds promise for addressing global food security challenges in a changing climate.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 5","pages":"2076-2088"},"PeriodicalIF":6.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AtDGCR14L contributes to salt-stress tolerance via regulating pre-mRNA splicing in Arabidopsis","authors":"Meng Xie,&nbsp;Dimiru Tadesse,&nbsp;Jin Zhang,&nbsp;Tao Yao,&nbsp;Li Zhang,&nbsp;Sara S. Jawdy,&nbsp;Amith Devireddy,&nbsp;Kaijie Zheng,&nbsp;Emily B. Smith,&nbsp;Jennifer Morrell-Falvey,&nbsp;Chongle Pan,&nbsp;Feng Chen,&nbsp;Gerald A. Tuskan,&nbsp;Wellington Muchero,&nbsp;Jin-Gui Chen","doi":"10.1111/tpj.17136","DOIUrl":"10.1111/tpj.17136","url":null,"abstract":"<div>\u0000 \u0000 <p>In plants, pre-mRNA alternative splicing has been demonstrated to be a crucial tier that regulates gene expression in response to salt stress. However, the underlying mechanisms remain elusive. Here, we studied the roles of DIGEORGE-SYNDROME CRITICAL REGION 14-like (AtDGCR14L) in regulating pre-mRNA splicing and salt stress tolerance. We discovered that Arabidopsis AtDGCR14L is required for maintaining plant salt stress tolerance and the constitutively spliced and active isoforms of important stress- and/or abscisic acid (ABA)-responsive genes. We also identified the interaction between AtDGCR14L and splicing factor U1-70k, which needs a highly conserved three amino acid (TWG) motif in DGCR14. Different from wild-type AtDGCR14L, the overexpression of the TWG-substituted AtDGCR14L mutant did not change salt stress tolerance or pre-mRNA splicing of stress/ABA-responsive genes. Additionally, SWITCH3A (SWI3A) is a core subunit of the SWI/SUCROSE NONFERMENTING (SWI/SNF) chromatin-remodeling complexes. We found that SWI3A, whose splicing depends on AtDGCR14L, actively enhances salt stress tolerance. These results revealed that AtDGCR14L may play an essential role in crosstalk between plant salt-stress response and pre-mRNA splicing mechanisms. We also unveiled the potential role of SWI3A in controlling salt stress tolerance. The TWG motif in the intrinsically disordered region of AtDGCR14L is highly conserved and crucial for DGCR14 functions.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2668-2682"},"PeriodicalIF":6.2,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}