The Plant Journal最新文献

{"title":"Hydrogen sulfide interferes with ethylene biosynthesis and signaling pathway in tomato by the mediation of SlERF.D2 persulfidation","authors":"Ying Sun,&nbsp;Min Zhang,&nbsp;Meihui Geng,&nbsp;Zhikun Geng,&nbsp;Zixu Lu,&nbsp;Nannan Liu,&nbsp;Zhanmin Liu,&nbsp;Dexin Zeng,&nbsp;Gaifang Yao,&nbsp;Kangdi Hu,&nbsp;Hua Zhang","doi":"10.1111/tpj.70000","DOIUrl":"https://doi.org/10.1111/tpj.70000","url":null,"abstract":"<div>\u0000 \u0000 <p>Hydrogen sulfide (H₂S), as a signaling molecule, is found to delay fruit ripening and senescence by antagonizing the biosynthesis and signaling of ethylene, whereas the mechanism remains unclear. In the current work, exogenous H₂S fumigation could alleviate tomato fruit ripening and an ethylene response factor SlERF.D2 was found to be persulfidated at Cys35 by mass spectrometry analysis. Meanwhile, ethylene biosynthesis related genes <i>SlACS1</i> and <i>SlACO3</i> were significantly downregulated at gene expression level in H₂S-treated fruit. By CRISPR/Cas9 and gene overexpression, we showed that overexpression of <i>SlERF.D2</i> promoted fruit ripening by accelerating chlorophyll degradation and carotenoid accumulation and upregulating the expression of ripening related genes <i>SlPAO</i>, <i>SlPPH</i>, <i>SlSGR1</i>, <i>SlACS1</i>, <i>SlACS2</i>, <i>SlACS4</i>, <i>SlEIN2</i>, <i>SlACO1</i>, and <i>SlACO3</i>, while the mutation of <i>slerf.d2</i> delayed fruit ripening. Additionally, <i>slerf.d2</i> mutant showed delayed ethylene production during tomato fruit ripening. Moreover, SlERF.D2 was found to interact with the kinase SlMAPK4 and was phosphorylated at Ser42 by yeast two-hybrid screening, pull down and LC–MS/MS. By cis-element analysis, electrophoretic mobility shift assay and dual-luciferase assay, SlERF.D2 could activate the transcription of the ethylene pathway-associated gene <i>SlACO3</i> and <i>SlEIN2</i>. Besides, we provided evidence that SlERF.D2 persulfidation weakened the transcriptional activity of SlERF.D2 on the target gene <i>SlACO3</i> and <i>SlEIN2</i>. In contrast, SlMAPK4-mediated phosphorylation enhanced SlERF.D2's transcriptional activation activity on <i>SlACO3</i> and <i>SlEIN2</i>. Therefore, the present research provides insights into the mechanism of H₂S in antagonizing the biosynthesis and signaling transduction of ethylene and reveals the importance of SlERF.D2 persulfidation and phosphorylation in dynamically regulating tomato fruit ripening.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular chaperon HSP70-9 is required for clathrin-mediated endocytosis in Arabidopsis","authors":"Tingting Shen,&nbsp;Zimeng Liu,&nbsp;Jiaoteng Bai,&nbsp;Xiaoting Zhai,&nbsp;Shanshan Wei,&nbsp;Wenyan Xu,&nbsp;Ning Jia,&nbsp;Bing Li","doi":"10.1111/tpj.17265","DOIUrl":"https://doi.org/10.1111/tpj.17265","url":null,"abstract":"<div>\u0000 \u0000 <p>Clathrin-mediated endocytosis (CME) plays a key role in the internalization of plasma membrane-localized proteins, lipids and extracellular substances; however, the regulatory mechanism of CME in plants is unclear. In this work, we demonstrated that molecular chaperon HSP70-9 is required for CME in <i>Arabidopsis thaliana</i>. Knocking out the <i>HSP70-9</i> gene led to auxin-related phenotypes and the accumulation of auxin efflux carrier PINs in root cells, indicating that HSP70-9 is involved in the PIN trafficking process. Immunolocalization studies showed that the HSP70-9 was localized in mitochondria, vesicles and cytoplasm. Next, we found that HSP70-9 and clathrin light chain 1 (CLC1) were co-localized in cells, and there was a strong interaction between the two. <i>HSP70-9</i> knockout led to an increase in CLC1-related vesicle number in root cells, and the application of protein synthesis inhibitor cycloheximide did not significantly inhibit the increase in the <i>hsp70-9a</i> mutant. <i>HSP70-9</i> knockout did not significantly affect the level of <i>CLC1</i> mRNA, but reduced the abundance of free CLC1 protein in root cells, indicating that HSP70-9 might be involved in the stability of CLC1-related vesicles. Moreover, our data indicated that the introduction of the exogenous <i>CLC1</i> gene rescued the <i>hsp70-9a</i> seedlings likely through promoting PIN trafficking. Furthermore, the role of HSP70-9 in the depolymerization of clathrin-coated vesicles (CCVs) was investigated <i>in vitro</i>. The result indicated that the addition of HSP70-9 promoted the release of CLC1 from the CCVs. Taken together, our data suggest that HSP70-9 affects PIN trafficking likely through facilitating the release of CLC1 from the CCVs.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome engineering points to the cis-acting mechanism of chromosome arm-specific telomere length setting and robustness of plant phenotype, chromatin structure and gene expression","authors":"Ondřej Helia,&nbsp;Barbora Matúšová,&nbsp;Kateřina Havlová,&nbsp;Anna Hýsková,&nbsp;Martin Lyčka,&nbsp;Natalja Beying,&nbsp;Holger Puchta,&nbsp;Jiří Fajkus,&nbsp;Miloslava Fojtová","doi":"10.1111/tpj.70024","DOIUrl":"https://doi.org/10.1111/tpj.70024","url":null,"abstract":"<p>The study investigates the impact of targeted chromosome engineering on telomere dynamics, chromatin structure, gene expression, and phenotypic stability in <i>Arabidopsis thaliana</i>. Using precise CRISPR/Cas-based engineering, reciprocal translocations of chromosome arms were introduced between non-homologous chromosomes. The subsequent homozygous generations of plants were assessed for phenotype, transcriptomic changes and chromatin modifications near translocation breakpoints, and telomere length maintenance. Phenotypically, translocated lines were indistinguishable from wild-type plants, as confirmed through morphological assessments and principal component analysis. Gene expression profiling detected minimal differential expression, with affected genes dispersed across the genome, indicating negligible transcriptional impact. Similarly, ChIPseq analysis showed no substantial alterations in the enrichment of key histone marks (H3K27me3, H3K4me1, H3K56ac) near junction sites or across the genome. Finally, bulk and arm-specific telomere lengths remained stable across multiple generations, except for minor variations in one translocation line. These findings highlight the remarkable genomic and phenotypic robustness of <i>A. thaliana</i> despite large-scale chromosomal rearrangements. The study offers insights into the <i>cis</i>-acting mechanisms underlying chromosome arm-specific telomere length setting and establishes the feasibility of chromosome engineering for studies of plant genome evolution and crop improvement strategies.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rice RuBisCO activase promotes the dark-induced leaf senescence by enhancing the degradation of filamentation temperature-sensitive H","authors":"Yanli Zhang,&nbsp;Guojun Dong,&nbsp;Xiaoyue Wu,&nbsp;Fei Chen,&nbsp;Banpu Ruan,&nbsp;Yaohuang Jiang,&nbsp;Ying Zhang,&nbsp;Lu Liu,&nbsp;Yao-Wu Yuan,&nbsp;Limin Wu,&nbsp;Jian Wei,&nbsp;Qian Qian,&nbsp;Yanchun Yu","doi":"10.1111/tpj.17267","DOIUrl":"https://doi.org/10.1111/tpj.17267","url":null,"abstract":"<div>\u0000 \u0000 <p>Leaf senescence is a complex process that is triggered by many developmental and environmental factors. However, the mechanisms regulating leaf senescence remain unclear. Here, we revealed that rice ribulose-1,5-bisphosphate carboxylase/oxygenase activase (RCA) promotes the onset of basal dark-induced senescence. <i>RCA</i> was mainly expressed in the leaves, and its expression level quickly declined under dark conditions. Furthermore, <i>rca</i> mutant plants presented a prolonged leaf longevity phenotype in the dark, whereas overexpression of the large isoform of RCA (<i>RCA</i>_<i>L</i>), not small isoform (<i>RCA</i>_<i>S</i>), in rice and Arabidopsis accelerated leaf senescence. Filamentation temperature-sensitive H (OsFtsH1), a zinc metalloprotease, interacts with RCA_L and RCA_S and presents a higher binding efficiency to RCA_L than RCA_S in darkness. Furthermore, we found that RCA_L promotes 26S proteasome-mediated degradation of OsFtsH1 protein, which can be inhibited by protease inhibitor MG132. Consequently, OsFtsH1 loss-of-function mutants exhibit accelerated leaf senescence, whereas <i>OsFtsH1</i>-overexpressing plants display delayed senescence. Collectively, our findings highlight the significant role of RCA_L isoform in regulating leaf senescence under dark conditions, particularly through enhancing the degradation of OsFtsH1.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrate activates an MKK3-dependent MAPK module via NLP transcription factors in Arabidopsis","authors":"Sebastian T. Schenk,&nbsp;Virginie Brehaut,&nbsp;Camille Chardin,&nbsp;Marie Boudsocq,&nbsp;Anne Marmagne,&nbsp;Jean Colcombet,&nbsp;Anne Krapp","doi":"10.1111/tpj.70010","DOIUrl":"https://doi.org/10.1111/tpj.70010","url":null,"abstract":"<p>Plant responses to nutrient availability are critical for plant development and yield. Nitrate, the major form of nitrogen in most soils, serves as both a nutrient and signaling molecule. Nitrate itself triggers rapid, major changes in gene expression, especially via nodule inception (NIN)-like protein (NLP) transcription factors, and stimulates protein phosphorylation. Mitogen-activated protein kinase (MAPK)-related genes are among the early nitrate-responsive genes; however, little is known about their roles in nitrate signaling pathways. Here, we show that nitrate resupply to nitrogen-depleted Arabidopsis (<i>Arabidopsis thaliana</i>) plants triggers, within minutes, an MAPK cascade that requires NLP-dependent transcriptional induction of <i>mitogen-activated protein kinase kinase kinase 13</i> (<i>MAP3K13</i>) and <i>MAP3K14</i> and that the MAPK cascade is composed of MKK3 and likely C-clade MAPKs (MPK1/2/7/14). Importantly, nitrate reductase-deficient mutants exhibited nitrate-induced MPK7 activities comparable to those observed in wild-type plants, indicating that nitrate itself is the signal that stimulates the cascade. We show that the modified expression of <i>MAP3K13</i> and <i>MAP3K14</i> affects nitrate-stimulated <i>BT2</i> expression and modulates plant responses to nitrogen availability, such as nitrate uptake and senescence. Our finding that an MAPK cascade involving MAP3K13 and MAP3K14 functions in the complex regulatory network governing responses to nitrate availability will guide future strategies to optimize plant responses to nitrogen fertilization and nitrogen use efficiency.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"At the grass roots: non-destructive root phenotyping using X-ray computed tomography","authors":"Gwendolyn K. Kirschner","doi":"10.1111/tpj.70030","DOIUrl":"https://doi.org/10.1111/tpj.70030","url":null,"abstract":"&lt;p&gt;Plant roots are crucial for accessing water and nutrients and directly impact crop yield. However, because they grow underground in the soil, their phenotyping is very difficult. To allow root visualization, artificial growth systems can be used, in which the plants are grown in a transparent substrate like agar, on filter paper, or in a hydroponic system (Li et al., &lt;span&gt;2022&lt;/span&gt;). The downside of these systems is that they only allow phenotyping at early growth stages, and traits observed in artificial systems often do not align with those in soil due to the complex nature of soil, which includes factors like soil compaction, uneven distribution of water and nutrients, and microbiota (Watt et al., &lt;span&gt;2013&lt;/span&gt;). Therefore, analyzing root traits under field conditions is crucial for identifying stable quantitative trait loci (QTLs) for agricultural applications.&lt;/p&gt;&lt;p&gt;Soil coring is commonly used for root phenotyping in the field. For that, a cylinder is inserted into the ground to extract a monolith of soil and the roots within it (Figure 1a) (Böhm, &lt;span&gt;1979&lt;/span&gt;). However, this method requires washing the soil off the roots and is therefore labor-intensive. This is particularly challenging in clay soils, such as those in rice paddy fields, where washing the roots is difficult. Additionally, when rice roots are removed from the soil, their structure collapses, making it impossible to determine their 3D structure.&lt;/p&gt;&lt;p&gt;Shota Teramoto and Yusaku Uga, the authors of the highlighted publication, developed an X-ray computed tomography (CT)-based method to analyze root structure from monolith samples without disturbing the soil core (Teramoto &amp; Uga, &lt;span&gt;2024&lt;/span&gt;). Uga's group at the National Agriculture &amp; Food Research Organization in Ibaraki, Japan, focuses on designing and developing climate-resilient crops by optimizing root system architecture to enhance adaptation to environmental stresses. One of their ongoing research projects involves developing advanced root phenotyping techniques. Within this project, they developed a method to visualize rice root systems grown in pots using X-ray CT. Teramoto, a Senior Researcher in the group, believed that applying this technology to rice root systems collected from paddy fields could solve the challenges of the phenotyping method.&lt;/p&gt;&lt;p&gt;For phenotyping, monolith samples were isolated from rice paddy fields, scanned using X-ray CT and analyzed using a workflow named RSApaddy3D (Figure 1b,c). The authors developed a special 2D kernel filter to isolate root-shaped fragments from the CT scans, optimized to detect small, dot-like fragments within its ring. Roots in soil appear as 3D tubular fragments creating a cross-section with a circular segment of the root in at least one of the &lt;i&gt;x&lt;/i&gt;-, &lt;i&gt;y&lt;/i&gt;-, or &lt;i&gt;z&lt;/i&gt;-axis planar slices. This segment can be detected with the 2D filter. If the ring diameter is larger than the root diameter, using this filter along all three axes and","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143404667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haplotype-resolved genome and population genomics provide insights into dioscin biosynthesis and evolutionary history of the medicinal species Dioscorea nipponica","authors":"Ke Hu,&nbsp;Yu Feng,&nbsp;Pan Li,&nbsp;Min Chen,&nbsp;Zi-Jie Shen,&nbsp;Xiao-Qin Sun,&nbsp;Rui-Sen Lu","doi":"10.1111/tpj.17237","DOIUrl":"https://doi.org/10.1111/tpj.17237","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Dioscorea nipponica</i>, a perennial herb widely distributed in the Sino-Japanese Floristic Region, is renowned for its medicinal properties, particularly its ability to produce dioscin. Here, we present a haplotype-resolved genome assembly of the diploid <i>D</i>. <i>nipponica</i>, comprising 511.41 Mb for Haplotype A and 498.29 Mb for Haplotype B, each organized into 10 chromosomes. The two haplotypes exhibited high similarity, with only 2.75% of the allelic genes exhibiting specific expression. Key genes in the dioscin biosynthesis pathway were identified, and expression analysis revealed that the majority (16/21) of genes involved in the first two stages were highly expressed in rhizomes. Notably, significant expansion of the CYP90, CYP94, and UGT73 gene families was observed in dioscin-producing species, highlighting their critical roles in dioscin biosynthesis. Additionally, genome size estimation and population genomic analyses of diverse <i>D. nipponica</i> accessions identified four principal clades in <i>D. nipponica</i>, corresponding to diploid, di-tetra-octoploid, tetraploid, and hexaploid accessions from various geographic regions, with clade A (diploids) further divided into five subclades. Demographic analysis of the diploid <i>D. nipponica</i> revealed a prolonged decline in effective population size from the Pleistocene to the Last Glacial Maximum, with population splits occurring during the mid-to-late Pleistocene. Selective sweep analysis identified key genes, including <i>HD-Zip I</i>, <i>ADH1</i>, <i>SMT1</i>, and <i>CYPs</i> that may contribute to adaptation to high-latitude environments and the geographical variations in dioscin content. Overall, this study enhances our understanding of the genomic architecture, biosynthetic pathways, and evolutionary dynamics of <i>D. nipponica</i>, providing valuable insights into its medicinal potential and evolution.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic dissection of internode length confers improvement for ideal plant architecture in maize","authors":"Haiyang Duan,&nbsp;Jianxin Li,&nbsp;Zhengjie Xue,&nbsp;Lu Yang,&nbsp;Yan Sun,&nbsp;Xiaolong Ju,&nbsp;Jihong Zhang,&nbsp;Guoqiang Xu,&nbsp;Xuehang Xiong,&nbsp;Li Sun,&nbsp;Shuhao Xu,&nbsp;Huiling Xie,&nbsp;Dong Ding,&nbsp;Xuebin Zhang,&nbsp;Xuehai Zhang,&nbsp;Jihua Tang","doi":"10.1111/tpj.17245","DOIUrl":"https://doi.org/10.1111/tpj.17245","url":null,"abstract":"<div>\u0000 \u0000 <p>The optimal plant architecture, characterized by short stature, helps mitigate lodging, enables high-density planting, and facilitates mechanized harvesting. Internode length (IL), a crucial component of plant height in maize, plays a significant role in these processes. However, the genetic mechanisms underlying internode elongation remain poorly understood. In this study, we conducted a genome-wide association study to dissect the genetic architecture of IL in maize. The lengths of five internodes above and below the ear (referred as IL-related traits) were collected across multiple environments, revealing substantial variation. A total of 108 quantitative trait loci (QTL) were associated with 11 IL-related traits, with 17 QTL co-detected by different traits. Notably, three QTL have been selected in maize breeding progress. Three hundred and three genes associated with IL were found to operate through plant hormone signal transduction, receptor activity, and carbon metabolism pathways, influencing internode elongation. <i>ZmIL1</i>, which encodes alcohol dehydrogenase, exhibited a high expression level in internodes during the vegetative stage and has been selected in Chinese modern maize breeding. Additionally, <i>ZmIL2</i> and <i>ZmIL3</i> emerged as other crucial regulators of IL. Importantly, <i>ZmIL1</i> has potential applications in maize varieties in the Huang-Huai-Hai region. This study represents the first comprehensive report on the genetic architecture of nearly all ILs in maize, providing profound insights into internode elongation mechanisms and genetic resources. These findings hold significant implications for dwarf breeding programs aimed at optimizing plant architecture for enhancing agronomic performance.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DREPP protein StPCaP1 facilitates the cell-to-cell movement of Potato virus Y and Potato virus S by inhibiting callose deposition at plasmodesmata","authors":"Ruhao Chen,&nbsp;Zhen Tu,&nbsp;Tao Yu,&nbsp;Zhaorong Wu,&nbsp;Saiful Islam,&nbsp;Xinxi Hu,&nbsp;Changzheng He,&nbsp;Botao Song,&nbsp;Qiusheng Kong,&nbsp;Bihua Nie","doi":"10.1111/tpj.17239","DOIUrl":"https://doi.org/10.1111/tpj.17239","url":null,"abstract":"<div>\u0000 \u0000 <p>Plant viruses, constrained by their limited genomic coding capacity, rely significantly on host factors for successful infection. Disruption of these essential host factors can confer resistance to viruses, with such factors categorized as susceptibility genes or recessive resistance genes. Recent research has identified developmentally regulated plasma membrane polypeptide (DREPP) proteins as susceptibility factors integral to the cell-to-cell movement of potyviruses. In the present study, we demonstrated that the silencing of <i>StPCaP1</i>, a <i>DREPP</i> gene in potato, confers novel resistance to both <i>Potato virus Y</i> (PVY, <i>Potyvirus</i>) and <i>Potato virus S</i> (PVS, <i>Carlavirus</i>). Interaction and subcellular localization analyses revealed that the movement proteins (MPs) of PVY (P3NPIPO) and PVS (TGB1) interact with StPCaP1, recruiting it to plasmodesmata (PD). Furthermore, transcriptome analysis and experimental validation indicated that compared to wild-type (WT) controls, <i>StPCaP1</i>-silenced lines exhibit significantly increased glucose content and elevated expression levels of several <i>UDP-glucosyltransferases</i> (<i>UGTs</i>), which are potential components of the callose synthesis complex. These findings suggest that StPCaP1 participates in callose deposition, as evidenced by the increased callose deposition at PD and reduced PD permeability observed in <i>StPCaP1</i>-silenced lines. Additionally, we found that <i>StPCaP1</i> expression in <i>Nicotiana benthamiana</i> led to reduced callose deposition at PD and promoted PVY-GFP cell-to-cell movement in <i>NbPCaP1</i>-silenced plants in a concentration-dependent manner, which suggests the changes in callose deposition at PD induced by StPCaP1 relates to viral cell-to-cell movement. This study provides a deeper understanding of DREPP-mediated viral movement and highlights potential targets for developing virus-resistant crops.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthocyanin biosynthesis and transport synergistically modulated by RcMYB75 and RcGSTFL11 play a pivotal role in the feedforward loop in response to drought stress","authors":"Mengni Ma,&nbsp;Runhui Li,&nbsp;Yajun Li,&nbsp;Wenhao Dai,&nbsp;Junzhong Shang,&nbsp;Yanhong He,&nbsp;Fayun Xiang,&nbsp;Yuanyuan Yang,&nbsp;Jihua Wang,&nbsp;Zifeng Huang,&nbsp;Hong Luo,&nbsp;Jie Zhang,&nbsp;Guogui Ning","doi":"10.1111/tpj.17240","DOIUrl":"https://doi.org/10.1111/tpj.17240","url":null,"abstract":"<div>\u0000 \u0000 <p>Anthocyanins, the important antioxidants and signaling molecules, are natural polyphenolic compounds widely present in plants and essential for plant defense. However, little is known about the mechanisms underlying plant anthocyanin accumulation in relation to drought stress. This study reveals that drought stress induces significant anthocyanin accumulation in <i>Rosa chinensis</i>, alongside an increase in the expression of the MYB transcription factor (TF) gene <i>RcMYB75</i> and the glutathione S-transferase (GST) gene <i>RcGSTFL11</i>. When overexpressed, <i>RcMYB75</i> markedly increases anthocyanin contents in both roses and tobaccos; conversely, reducing its expression significantly lowers anthocyanin contents in rose petals. RcGSTFL11 was confirmed as an anthocyanin transporter and overexpression of <i>RcGSTFL11</i> can restore the anthocyanin-deficient phenotype in the Arabidopsis <i>tt19</i> mutant. Transgenic roses overexpressing <i>RcGSTFL11</i> exhibit enhanced anthocyanin accumulation, while those with downregulated <i>RcGSTFL11</i> have reduced contents. Transcriptomic analysis indicates that <i>RcMYB75</i> upregulates the expression of key genes in the anthocyanin biosynthetic pathway and the anthocyanin transport gene <i>RcGSTFL11.</i> Ultimately, we also found that anthocyanin accumulation in these transgenics further enhances plant resistance to drought stress. Taken together, RcMYB75 and RcGSTFL11 promote the synthesis and transport of anthocyanins and play a key role in the feedforward loop responding to drought stress in roses. This study provides insights into the molecular mechanisms by which MYB TFs contribute to anthocyanin biosynthesis and transport, as well as the adaptive strategies of roses in response to drought stress.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}