The Plant Journal最新文献

{"title":"Multi-omic analyses unveil contrasting composition and spatial distribution of specialized metabolites in seeds of Camelina sativa and other Brassicaceae","authors":"Léa Barreda,&nbsp;Céline Brosse,&nbsp;Stéphanie Boutet,&nbsp;Nicolas Klewko,&nbsp;Delphine De Vos,&nbsp;Tracy Francois,&nbsp;Boris Collet,&nbsp;Damaris Grain,&nbsp;Céline Boulard,&nbsp;Jean Chrisologue Totozafy,&nbsp;Benoît Bernay,&nbsp;François Perreau,&nbsp;Loïc Lepiniec,&nbsp;Loïc Rajjou,&nbsp;Massimiliano Corso","doi":"10.1111/tpj.17231","DOIUrl":"https://doi.org/10.1111/tpj.17231","url":null,"abstract":"<div>\u0000 \u0000 <p>Seeds of Brassicaceae produce a large diversity of beneficial and antinutritional specialized metabolites (SMs) that influence their quality and provide resistance to stresses. While SM distribution has been described in leaves and root tissues, limited information is available about their spatiotemporal accumulation in seeds. <i>Camelina sativa</i> (camelina) is an oilseed Brassicaceae cultivated for human and animal nutrition and for industrial uses. While we previously explored SM diversity and plasticity, no information is available about SM distribution and expression of related proteins and genes in camelina seeds. In this study, we used a multi-omic approach, integrating untargeted metabolomics, proteomics, and transcriptomics to investigate the synthesis, modification, and degradation of SMs accumulated in camelina seed tissues (seed coat, endosperm, embryo) at six developmental and two germination stages. Metabolomic results showed distinct patterns of SMs and their related pathways, highlighting significant contrasts in seed composition and spatial distribution for the defense-related and antinutritional glucosinolate (GSL) compounds among camelina, <i>Arabidopsis thaliana</i>, and <i>Brassica napus</i>, three closely related Brassicaceae species. Notably, thanks to metabolomic and proteomic/transcriptomic techniques the variation in GSL spatial distributions was primarily driven by differences in their structure (metabolomics data) and transport (transcriptomic and proteomic data) mechanisms. Long-chain C8–C11 methylsulfinylalkyl GSLs were predominantly accumulated in the seed coat and endosperm, while mid- and short-chain C3–C7 methylsulfinylalkyl GSLs were accumulated in the embryo. Characterizing the spatial dynamics of seed SMs provides valuable insights that can guide the development of crops with optimized distribution of beneficial and toxic metabolites, improving seed nutritional profiles.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The DNA methylomes of Echinochloa species provide insights into polyploidization-driven adaptation and orphan crop domestication","authors":"Qinjie Chu,&nbsp;Xiaojiao Gong,&nbsp;Yiyu Hu,&nbsp;Qian-Hao Zhu,&nbsp;Longjiang Fan,&nbsp;Chu-Yu Ye","doi":"10.1111/tpj.70033","DOIUrl":"https://doi.org/10.1111/tpj.70033","url":null,"abstract":"<div>\u0000 \u0000 <p>The genus <i>Echinochloa</i> (Poaceae) contains problematic weeds worldwide and two domesticated orphan crops. In this study, through sequencing DNA methylomes and transcriptomes, we aimed to reveal the epigenome changes and their relationship with gene expression in <i>Echinochloa</i> species during their polyploidization and domestication. Compared with hexaploid crop bread wheat, we found common and distinctive methylation patterns in hexaploid <i>Echinochloa crus-galli</i>. More diverse methylation patterns were uncovered during hexaploidization of <i>E. crus-galli</i>, suggesting more plasticity of the weed genome, which might contribute to its environmental adaptation. In addition, less changes in DNA methylation were observed in the two cultivated <i>Echinochloa</i> species, as compared with rice, indicating incomplete domestication of the <i>Echinochloa</i> orphan crops. Our results provide new insights into plant polyploidization and orphan crop domestication from an epigenomic perspective.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRF12 specifically regulates the flowering time of Arabidopsis thaliana under non-inductive conditions","authors":"Xia Li,&nbsp;Siyu Fang,&nbsp;Wanqin Chen,&nbsp;Siyuan Liu,&nbsp;Lirong Zhao,&nbsp;Zhiyu Xu,&nbsp;Shidie Chen,&nbsp;Yunwei Liu,&nbsp;Yang Du,&nbsp;Luyao Deng,&nbsp;Lei Liu,&nbsp;Ting Wang,&nbsp;Pingping Li,&nbsp;Yi Zhu,&nbsp;Diqiu Yu,&nbsp;Houping Wang","doi":"10.1111/tpj.17257","DOIUrl":"https://doi.org/10.1111/tpj.17257","url":null,"abstract":"<div>\u0000 \u0000 <p>The flowering time of <i>Arabidopsis thaliana</i>, a model plant, is significantly accelerated when exposed to long-day (LD) conditions, as it is a typical LD plant. Consequently, the investigation of the flowering regulatory network in <i>A. thaliana</i> under LD conditions has garnered considerable attention in the study of flowering signals, resulting in a significant breakthrough. While many LD plants, including <i>A. thaliana</i>, exhibit delayed flowering under non-inductive short-day (SD) conditions, they are still capable of flowering. Nevertheless, research on the regulation of flowering induction in LD plants under non-inductive SD conditions has been limited. This study demonstrated the involvement of CYTOKININ RESPONSE FACTORS 12 (CRF12) in the regulation of flowering in <i>A. thaliana</i> under non-inductive conditions. Analysis of the expression patterns revealed that the activation of <i>CRF12</i> expression and protein stability occurred exclusively in non-inductive environments. Molecular and genetic analyses revealed that under a non-inductive photoperiod of 12 h of light and 12 h of darkness, CRF12, CONSTANS (CO), and TARGET OF EAT 1/2 (TOE1/2) engage in competitive interactions to regulate flowering time, while in a SD photoperiod of 8 h of light and 16 h of darkness, CRF12 modulates flowering time by inhibiting the activity of TOE1/2.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial root-zone drying irrigation enhances synthesis of glutathione in barley roots to improve low temperature tolerance","authors":"Peng Mu,&nbsp;Fan Ye,&nbsp;Xintong Liu,&nbsp;Peng Zhang,&nbsp;Tianhao Liu,&nbsp;Xiangnan Li","doi":"10.1111/tpj.70026","DOIUrl":"https://doi.org/10.1111/tpj.70026","url":null,"abstract":"<div>\u0000 \u0000 <p>Partial root-zone drying irrigation (PRD) has been widely employed to regulate crop root development and responses to environmental fluctuations. However, its role in reprogramming rhizospheric microorganisms and inducing plant stress tolerance remains largely unexplored. This study aimed to investigate the effects of PRD on the response of barley (<i>Hordeum vulgare</i>) plants to low temperatures under various irrigation regimes. Under low temperature, barley plants subjected to PRD exhibited a significantly enhanced net photosynthetic rate, stomatal conductance, and maximum quantum efficiency of photosystem II compared to fully irrigated plants. Additionally, these plants showed a reduction in relative conductance. These results suggest that PRD could be a viable strategy for enhancing crop stress tolerance through irrigation management. Metabolomic analysis revealed that PRD influenced the accumulation of glutathione and 9-octadecenamide in roots under low temperature, which was corroborated by transcriptome profiling data. Furthermore, the study highlighted the close association between this regulatory process and rhizosphere core microorganisms, such as <i>Sphingobium</i> and <i>Mortierella</i>, enriched in barley roots under PRD. This study revealed the mechanism underlying plant stress tolerance induction by PRD and the roles of rhizosphere microorganisms in this process. Also, the current study suggests that PRD is a promising strategy for enhancing crop stress tolerance through effective irrigation management.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PlantGENE: Advancing plant transformation through community engagement","authors":"Aimee A. Malzahn,&nbsp;Heidi Kaeppler,&nbsp;William Gordon-Kamm,&nbsp;Keunsub Lee,&nbsp;Nigel Taylor,&nbsp;Veena Veena,&nbsp;Wayne Parrott,&nbsp;Joyce Van Eck","doi":"10.1111/tpj.17228","DOIUrl":"https://doi.org/10.1111/tpj.17228","url":null,"abstract":"<p>Plant transformation is an important part of plant research and crop improvement. Transformation methods remain complex, labor intensive, and inefficient. PlantGENE is a community of scientists from academia, industry, non-profit research institutes, and government organizations working to improve plant transformation. PlantGENE hosts virtual training, interactive webinars, and a website with career opportunities, directories, and more. The plant science community has shown great interest and support for PlantGENE.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17228","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting photosynthesis opens new opportunities for agriculture sustainability and circular economy: The BEST-CROP research and innovation action","authors":"Paolo Pesaresi,&nbsp;Pierre Bono,&nbsp;Stephane Corn,&nbsp;Cristina Crosatti,&nbsp;Sara Daniotti,&nbsp;Jens Due Jensen,&nbsp;Ivo Frébort,&nbsp;Eder Groli,&nbsp;Claire Halpin,&nbsp;Mats Hansson,&nbsp;Goetz Hensel,&nbsp;David S. Horner,&nbsp;Kelly Houston,&nbsp;Ahmed Jahoor,&nbsp;Miloš Klíma,&nbsp;Hannes Kollist,&nbsp;Clément Lacoste,&nbsp;Boubker Laidoudi,&nbsp;Susanna Larocca,&nbsp;Caterina Marè,&nbsp;Nicolas Le Moigne,&nbsp;Chiara Mizzotti,&nbsp;Tomas Morosinotto,&nbsp;Klaus Oldach,&nbsp;Laura Rossini,&nbsp;Sebastian Raubach,&nbsp;Miguel Sanchez-Garcia,&nbsp;Paul D. Shaw,&nbsp;Rodolphe Sonnier,&nbsp;Alessandro Tondelli,&nbsp;Robbie Waugh,&nbsp;Andreas P.M. Weber,&nbsp;Dmitry Yarmolinsky,&nbsp;Alessandro Zeni,&nbsp;Luigi Cattivelli","doi":"10.1111/tpj.17264","DOIUrl":"https://doi.org/10.1111/tpj.17264","url":null,"abstract":"<p>There is a need for ground-breaking technologies to boost crop yield, both grains and biomass, and their processing into economically competitive materials. Novel cereals with enhanced photosynthesis and assimilation of greenhouse gasses, such as carbon dioxide and ozone, and tailored straw suitable for industrial manufacturing, open a new perspective for the circular economy. Here we describe the vision, strategies, and objectives of BEST-CROP, a Horizon-Europe and United Kingdom Research and Innovation (UKRI) funded project that relies on an alliance of academic plant scientists teaming up with plant breeding companies and straw processing companies to use the major advances in photosynthetic knowledge to improve barley biomass and to exploit the variability of barley straw quality and composition. We adopt the most promising strategies to improve the photosynthetic properties and ozone assimilation capacity of barley: (i) tuning leaf chlorophyll content and modifying canopy architecture; (ii) increasing the kinetics of photosynthetic responses to changes in irradiance; (iii) introducing photorespiration bypasses; (iv) modulating stomatal opening, thus increasing the rate of carbon dioxide fixation and ozone assimilation. We expect that by improving our targeted traits we will achieve increases in aboveground total biomass production without modification of the harvest index, with added benefits in sustainability via better resource-use efficiency of water and nitrogen. In parallel, the resulting barley straw is tailored to: (i) increase straw protein content to make it suitable for the development of alternative biolubricants and feed sources; (ii) control cellulose/lignin contents and lignin properties to develop straw-based construction panels and polymer composites. Overall, by exploiting natural- and induced-genetic variability as well as gene editing and transgenic engineering, BEST-CROP will lead to multi-purpose next generation barley cultivars supporting sustainable agriculture and capable of straw-based applications.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17264","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation of genome-wide DNA methylation between japonica and indica rice","authors":"Tao Yan,&nbsp;Liuhui Kuang,&nbsp;Fei Gao,&nbsp;Jian Chen,&nbsp;Lin Li,&nbsp;Dezhi Wu","doi":"10.1111/tpj.17218","DOIUrl":"10.1111/tpj.17218","url":null,"abstract":"<div>\u0000 \u0000 <p>Rice (<i>Oryza sativa</i> L.) subspecies <i>japonica</i> and <i>indica</i> show distinct morphological and genetic differentiation. However, the differences in the genome-wide DNA methylation and its effects on gene expression and metabolic levels between <i>japonica</i> and <i>indica</i> rice remain unclear. In this study, we investigated the genome-wide DNA methylation, transcriptomes and metabolomes of 12 representative <i>japonica</i> and <i>indica</i> rice accessions, to reveal the differentiation between rice subspecies. We detected 83 327 differentially methylated regions (DMRs) and 14 903 DMR-associated genes between two subspecies. <i>Indica</i> rice showed significantly lower levels of the CG, CHG, and CHH methylation compared with <i>japonica</i> rice. Subsequently, we identified 5596 differentially expressed genes between the two subspecies, predominantly enriched in pathways related to carbohydrate and amino acid metabolism. By integrating DNA methylation with transcriptomic data, a significant correlation was established between methylation patterns and the expression level of key agronomic genes in rice. Furthermore, multi-omics analyses reveal that carbohydrate metabolism pathways, especially the tricarboxylic acid (TCA) cycle metabolites, are remarkable differentiation between rice subspecies. These results provide a foundation for future studies in rice domestication and genetic improvement.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Q&A with Dr. Adam Steinbrenner","authors":"","doi":"10.1111/tpj.70022","DOIUrl":"10.1111/tpj.70022","url":null,"abstract":"&lt;p&gt;&lt;i&gt;Logline for carousel:&lt;/i&gt; Dr. Steinbrenner shares his experiences in academia, and navigating biology in a family of non-academics. He shares his experiences as an assistant professor and gives insight into his research field on plant immunity.&lt;/p&gt;&lt;p&gt;&lt;i&gt;Subtitle for latest feature list:&lt;/i&gt; Adam Steinbrenner is an Associate Professor in the Department of Biology at the University of Washington, USA. He was recently appointed as part of The Plant Journal Editorial board.&lt;/p&gt;&lt;p&gt;@ADSteinbrenner&lt;/p&gt;&lt;p&gt;steinbrennerlab.org/&lt;/p&gt;&lt;p&gt;Dr. Steinbrenner is a passionate plant biologist whose journey into science is as fascinating as the questions he explores in his research. Growing up with a love for gardening and identifying trees, Dr. Steinbrenner discovered his calling through transformative research experiences in college, which introduced him to the molecular tools and ecological complexities of plant biology. Now leading his own lab, Dr. Steinbrenner focuses on pattern recognition receptors and how plants perceive and respond to diverse attackers like pathogens and herbivores. With a commitment to understanding the evolution of plant immune systems and addressing the challenges of receptor-ligand specificity, his work is advancing the frontiers of plant biology. Beyond the lab, Dr. Steinbrenner finds joy in discovery, mentoring the next generation of scientists, and balancing a fulfilling personal life with scientific pursuits.&lt;/p&gt;&lt;p&gt;\u0000 \u0000 &lt;/p&gt;&lt;p&gt;1. Can you tell us about you, your childhood, and your educational background? Anything that you're comfortable sharing.&lt;/p&gt;&lt;p&gt;As a kid, I loved gardening with my mom and identifying Pennsylvania trees. I did not know how it could become a career. Nobody in my family was in science or academia, so I remember being surprised and excited that there were research labs focused on questions in plant molecular biology.&lt;/p&gt;&lt;p&gt;\u0000 \u0000 &lt;/p&gt;&lt;p&gt;2. How did you become interested in plant biology?&lt;/p&gt;&lt;p&gt;I had two important research experiences in early college. At Tufts University I learned about plant specialized metabolism and ecological consequences working with Colin Orians. A summer NSF-REU internship at the Boyce Thompson Institute working with Greg Martin introduced me to model systems and molecular tools. It was 2007, the year after the famous zig-zag model of plant immunity was published – I remember discussing the model with Greg that summer. I was hooked.&lt;/p&gt;&lt;p&gt;\u0000 \u0000 &lt;/p&gt;&lt;p&gt;3. What are your current research interests?&lt;/p&gt;&lt;p&gt;My lab studies pattern recognition receptors (PRRs). We are interested in how these receptors perceive diverse attackers, especially chewing insect herbivores. We also want to know how signaling diverges coming from different PRRs, for example, PRRs that detect pathogens versus herbivores. We have built a model system based on plant perception of a caterpillar peptide (inceptin, also termed “In11”) mediated by a PRR called Inceptin","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping up: miR319 and LANCEOLATE control tomato fruit morphology","authors":"Martin Balcerowicz","doi":"10.1111/tpj.70002","DOIUrl":"10.1111/tpj.70002","url":null,"abstract":"&lt;p&gt;The domestication of tomato (&lt;i&gt;Solanum lycopersicum&lt;/i&gt;) has given rise to a wide range of cultivars with distinct fruit shapes and sizes. These traits are not only relevant to consumer preferences, often indicating culinary applications, but also bear importance for mechanical harvesting. Thus, understanding the molecular basis of fruit formation is a priority for scientists and breeders alike. Yet, few genes have been identified that regulate tomato fruit morphology. Among these, the transcriptional repressor &lt;i&gt;OVATE&lt;/i&gt; is one of the best characterised. Loss of &lt;i&gt;OVATE&lt;/i&gt; function causes increased cell division along the proximal-distal axis and reduced cell proliferation along the medial-lateral axis, resulting in elongated, pear-shaped fruits (Snouffer et al., &lt;span&gt;2020&lt;/span&gt;). How &lt;i&gt;OVATE&lt;/i&gt; itself is regulated, however, is unclear.&lt;/p&gt;&lt;p&gt;Small RNAs, especially microRNAs (miRNAs) and phased secondary small interfering RNAs (phasiRNAs), are critical modulators of fruit development (Huang et al., &lt;span&gt;2022&lt;/span&gt;). In the model plant &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;, miR319a affects the development of petals, stamen and siliques by targeting the mRNA encoding transcription factors TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) 3 and TCP4 (Cao et al., &lt;span&gt;2022&lt;/span&gt;; Nag et al., &lt;span&gt;2009&lt;/span&gt;). In tomato, miR160, miR166 and miR396 have been implicated in the regulation of fruit size and shape (Huang et al., &lt;span&gt;2022&lt;/span&gt;). Tomato miR319 and its target SlTCP2/LANCEOLATE (LA), on the other hand, act as important regulators of leaf morphology (Ori et al., &lt;span&gt;2007&lt;/span&gt;); their role in fruit formation had not been explored.&lt;/p&gt;&lt;p&gt;Fabio Nogueira developed a passion for miRNAs and their role in plant development during his postdoctoral studies with Marja Timmermans at Cold Spring Harbor Laboratory. After returning to Brazil to establish his own research group, he focused on the control of reproductive development, contrasting miRNA function in Arabidopsis with that in crops such as tomato. The highlighted study began as a Master's project of co-first author Airton Carvalho Jr, who investigated the links between tomato fruit shape, &lt;i&gt;LA&lt;/i&gt; and miR319.&lt;/p&gt;&lt;p&gt;Carvalho et al. characterised &lt;i&gt;La-1&lt;/i&gt; mutants, which harbour a mutation in &lt;i&gt;LA&lt;/i&gt;'s miR319 recognition site, which results in LA de-repression and ectopic expression (Ori et al., &lt;span&gt;2007&lt;/span&gt;). While homozygous mutants were rarely viable, heterozygous &lt;i&gt;La-1&lt;/i&gt;/+ plants produced elongated fruits with a malformed septum, a rudimental placenta and, in some genetic backgrounds, a reduced number of seeds (Figure 1a). These phenotypes can be traced back to changes in the morphology of &lt;i&gt;La-1&lt;/i&gt;/+ flowers, which have shorter sepals as well as carpels with a medial constriction in the ovary (Figure 1b). These phenotypes were associated with deregulated cell division in the developing flower and fruit: &lt;i&gt;La-1&lt;/i&gt;/+ plants displayed an increased number of cell layers in the","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmDREB1A controls plant immunity via regulating salicylic acid metabolism in maize","authors":"Chunxia Zhang,&nbsp;Huanbo Zhang,&nbsp;Wanping Lin,&nbsp;Jiahao Chai,&nbsp;Xiaoqing Shangguan,&nbsp;Tianyong Zhao","doi":"10.1111/tpj.17226","DOIUrl":"10.1111/tpj.17226","url":null,"abstract":"<div>\u0000 \u0000 <p>DREB1A, a pivotal transcription factor, has long been known to regulate plant abiotic stress tolerance. However, its role in plant biotic stress tolerance and the underlying mechanisms have remained a mystery. Our research reveals that the maize <i>ZmDREB1A</i> gene is up-regulated in maize seedlings when the plants are infected by <i>Rhizoctonia solani</i> (<i>R. solani</i>). The maize <i>ZmDREB1A</i> knock-out mutant exhibits increased disease resistance against the pathogen <i>R. solani</i>. Further investigation showed that ZmDREB1A regulates salicylic acid (SA) metabolism by inhibiting <i>ZmSARD1</i> gene and activating <i>ZmSAGT</i> gene expression. Additionally, the SA level was increased while the SAG level was decreased in <i>zmdreb1a</i> mutant seedlings when the plants were infected with the pathogen <i>R. solani</i>. Furthermore, overexpression of <i>ZmSAGT</i> in <i>Arabidopsis</i> reduced plant resistance to <i>Pst</i> DC3000 by decreasing SA levels and increasing SAG levels. These data demonstrate that ZmDREB1A regulates the metabolism of SA and controls plant immune response in maize.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}