The Plant Journal最新文献

{"title":"Characterization of an α-ketoglutarate-dependent oxygenase involved in converting 2-(2-phenylethyl)chromones into 2-styrylchromones in agarwood","authors":"Mingliang Zhang,&nbsp;Jiangping Fan,&nbsp;Zekun Zhang,&nbsp;Mengrong Niu,&nbsp;Xinyu Mi,&nbsp;Hailing Qiu,&nbsp;Jun Li,&nbsp;Xiao Liu,&nbsp;Juan Wang,&nbsp;Xiaohui Wang,&nbsp;Pengfei Tu,&nbsp;She-Po Shi","doi":"10.1111/tpj.70068","DOIUrl":"https://doi.org/10.1111/tpj.70068","url":null,"abstract":"<div>\u0000 \u0000 <p>2-Phenylethylchromones (PECs) and 2-styrylchromones (SCs) are the primary components responsible for the delightful fragrance and bioactivity of agarwood, a highly valuable aromatic resinous heartwood. PECs are derived from a common precursor with a diarylpentanoid skeleton (C₆–C₅–C₆). However, the biosynthesis of SCs remains unclear. In this study, based on the successful conversion of the PEC skeleton, rather than a dehydrogenated diarylpentanoid, into SCs by <i>Aquilaria sinensis</i> suspension cells, we demonstrated that double bond formation of the styryl group in SCs occurs after the creation of the PEC skeleton, not before this step from a dehydrogenated diarylpentanoid precursor. Through transcriptomic data mining, transient expression in <i>Nicotiana benthamiana</i> and <i>A. sinensis</i> suspension cells, we identified a new 2-oxoglutarate-dependent oxygenase (<i>As</i>2OG1) that plays a crucial role in the conversion of PECs into SCs. Further protein structure prediction and mutagenesis studies, combined with probing of the catalytic potential of <i>As</i>2OG1 using chemically synthesized hydroxylated intermediates, suggested that <i>As</i>2OG1 possibly uses diradical or carbocation intermediates, rather than hydroxylated intermediates, to install double bonds in SCs. The results not only provide insights into the molecular mechanism of agarwood formation but also facilitate the overproduction of pharmaceutically important SCs using metabolic engineering approaches.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing the rice ideotype: Optimizing traits for methane mitigation and sustainable yield","authors":"Saleem Asif,&nbsp;Yoon-Hee Jang,&nbsp;Rahmatullah Jan,&nbsp;Sajjad Asaf,&nbsp; Lubna,&nbsp;Eun-Gyeong Kim,&nbsp;Jae-Ryoung Park,&nbsp;Kyung-Min Kim","doi":"10.1111/tpj.70087","DOIUrl":"https://doi.org/10.1111/tpj.70087","url":null,"abstract":"<div>\u0000 \u0000 <p>Rice is a staple food for billions of people but also a major source of methane emissions, contributing approximately 10% of global agricultural methane. Therefore, this study aimed to conduct a correlation analysis of various traits gathered from years of research on the 120 Cheongcheong Nagdong Double Haploid (CNDH) population to identify key traits responsible for methane emission in rice. This study focused on practical plant traits, including culm length, spikelets per panicle, and grain weight, which have a positive correlation with methane emission. Shorter culm lengths produce less biomass, thereby reducing the organic matter available to feed methane-producing microbes. Increasing the number of spikelets per panicle increase boosts grain production, thereby reducing the development of root exudates that contribute to methane production. Our results indicate a positive correlation (<i>r</i> = 0.51) between grain weight and methane emissions, suggesting that selecting for heavier grains may actually increase methane emissions. Based on these features, we propose an rice ideotype variety that possibly minimizes the rice plant methane emissions while maintaining a high yield. This paper suggests that future studies should be extended to validate these current findings and explore the genetic components and ecological aspects of methane emissions to improve methane management in sustainable rice farming systems.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capsid protein of turnip crinkle virus suppresses antiviral RNA decay by degrading Arabidopsis Dcp1 via ubiquitination pathway","authors":"Kunxin Wu,&nbsp;Qiuxian Xie,&nbsp;Xueting Liu,&nbsp;Yan Fu,&nbsp;Shuxia Li,&nbsp;Xiaoling Yu,&nbsp;Wenbin Li,&nbsp;Pingjuan Zhao,&nbsp;Yanli Ren,&nbsp;Mengbin Ruan,&nbsp;Xiuchun Zhang","doi":"10.1111/tpj.70075","DOIUrl":"https://doi.org/10.1111/tpj.70075","url":null,"abstract":"<div>\u0000 \u0000 <p>RNA decay is a pervasive process in eukaryotic cells. Viruses utilize the host cell's intracellular machinery to gain access to essential molecules and subcellular structures required for infection during the pathogenesis process. The study demonstrates that turnip crinkle virus (TCV) infection enhances the expression of Arabidopsis Dcp1 (AtDcp1), which negatively regulates the accumulation of TCV RNA, indicating its involvement in antiviral defense. Nevertheless, TCV circumvents the antiviral defense based on RNA decay, as indicated by the capsid protein (CP) of TCV stabilizing the known nonsense-mediated RNA decay-targeted transcripts. <i>In vivo</i>, CP physically interacts with AtDcp1, promoting AtDcp1 degradation via ubiquitination pathway. This is evidenced by the observation that the degradation is inhibited by 26S proteasome inhibitors. Furthermore, CP elevates the polyubiquitination of Dcp1-Flag. These data indicate that CP suppresses RNA decay by interacting with AtDcp1 and mediating its degradation through the 26S proteasome pathway, effectively suppressing antiviral RNA decay. This study uncovers a previously unidentified virulence strategy in the ongoing conflict between plants and TCV.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A node-localized transporter TaSPDT is responsible for the distribution of phosphorus to grains in wheat","authors":"Aiying Wang,&nbsp;Yaoke Duan,&nbsp;Rong Wang,&nbsp;Shuang Li,&nbsp;Keqiao Cui,&nbsp;Xiaoping Kong,&nbsp;Feijuan Gao,&nbsp;Bochao He,&nbsp;Zhen Jiao,&nbsp;Hao Sun","doi":"10.1111/tpj.70065","DOIUrl":"https://doi.org/10.1111/tpj.70065","url":null,"abstract":"<div>\u0000 \u0000 <p>Wheat (<i>Triticum aestivum</i> L.) is one of the world's main food crops and the largest phosphorus (P) fertilizer consumer globally. However, the molecular mechanism of P distribution in wheat remains largely unknown. This study investigated the <i>TaSULTR</i> gene family and functionally characterized <i>TaSPDT</i> (<i>TaSULTR3;4</i>). Thirty-three <i>TaSULTR</i> genes were identified and divided into four groups. These genes contained three tandem duplications and 28 segmental duplications. TaSPDT was localized on the plasma membrane and demonstrated P transport activity. <i>TaSPDT</i> was mainly expressed at nodes, and its expression was elevated under low P conditions. TaSPDT was distributed on the xylem and phloem of enlarged and diffuse vascular bundles at nodes, as well as on the parenchyma cell bridge between vascular bundles. <i>TaSPDT</i> knockout reduced P distribution to young leaves but increased it in older leaves during the vegetative stage under low P availability. P uptake by roots, transfer to above-ground tissues, and redistribution within aerial organs were unaffected. At the reproductive stage, TaSPDT knockout notably diminished P allocation to grains, resulting in a significant decrease in grain yield, particularly under P-deficient conditions. These results suggest that TaSPDT mediates the transmembrane transport of P from the xylem to the phloem at the nodes, resulting in the preferential distribution of P to grains. This study enables a better understanding of the <i>TaSULTR</i> gene family and P distribution in wheat.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryptochrome 1 promotes photomorphogenesis in Arabidopsis by displacing substrates from the COP1 ubiquitin ligase","authors":"Laura Trimborn,&nbsp;Franziska Kuttig,&nbsp;Jathish Ponnu,&nbsp;Pengxin Yu,&nbsp;Kris R. Korsching,&nbsp;Patrick Lederer,&nbsp;Uriel Urquiza-García,&nbsp;Matias D. Zurbriggen,&nbsp;Ute Hoecker","doi":"10.1111/tpj.70071","DOIUrl":"https://doi.org/10.1111/tpj.70071","url":null,"abstract":"<p>In blue light, cryptochrome photoreceptors inhibit the key repressor of light signaling, the COP1/SPA ubiquitin ligase, to promote photomorphogenic responses. This inhibition relies on the direct interaction between COP1 and cryptochromes. Here, we analyzed the molecular mechanism of CRY1-mediated inhibition of COP1. We show that the VP motif in the C-terminal domain of CRY1 is essential for the COP1-CRY1 interaction in Arabidopsis. Phenotypic analysis of transgenic Arabidopsis plants harboring a mutation in the VP motif reveals that the VP motif of CRY1 is required for blue light-induced responses, such as seedling de-etiolation and anthocyanin biosynthesis. Via its VP motif, CRY1 inhibits the interaction between COP1 and the COP1 substrate transcription factors PAP2 and HY5. Replacing the VP motif of CRY1 with that of the human COP1 interactor TRIB1 produces a functional photoreceptor in transgenic plants. Since HY5, PAP2 and CRY1 interact with COP1 through their respective VP motifs, our results demonstrate that CRY1 inhibits the activity of COP1 by competitively displacing substrates from COP1. Taken together with previous results showing VP-dependent substrate displacement by photoactivated CRY2 and UVR8 photoreceptors, our results highlight the conservation of this mechanism across multiple photoreceptors.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MdPHR2 and MdARF6-4 synergistically regulate arbuscular mycorrhizal symbiosis and the transcription of MdPHT1;13, enhancing phosphorus uptake in apple rootstocks","authors":"Yimei Huang,&nbsp;Longmei Zhai,&nbsp;Yan Zhou,&nbsp;Jiahong Lv,&nbsp;Yao Liu,&nbsp;Ting Wu,&nbsp;Xinzhong Zhang,&nbsp;Zhenhai Han,&nbsp;Yi Wang","doi":"10.1111/tpj.70070","DOIUrl":"https://doi.org/10.1111/tpj.70070","url":null,"abstract":"<div>\u0000 \u0000 <p>Phosphorus in the soil is easily chelated into forms that are unavailable to plants, leading to phosphorus deficiency, which severely affects the growth, development, and fruit quality of apple trees. To address phosphorus deficiency, we used four different arbuscular mycorrhizal fungi (AMF) to investigate their effects on the growth and development of apple rootstocks and phosphorus uptake in the soil. We identified <i>Glomus mosseae</i> (<i>Gm</i>) fungi as the most effective AMF for promoting growth and found that under phosphorus-deficient conditions, inoculating with <i>Gm</i> fungi promoted the growth of the above-ground parts of the plants and phosphorus absorption, while it inhibited root growth. After inoculating with <i>Gm</i> fungi, we found phosphorus starvation response factors (PHRs) and auxin response factors (ARFs) were upregulated. Knockdown of <i>MdPHR2</i> or <i>MdARF6-4</i> resulted in decreased root arbuscular structures, total mycorrhizal colonization rate, and root phosphorus content, indicating that MdPHR2 and MdARF6-4 positively regulate the symbiosis of <i>Gm</i> fungi and phosphorus absorption. In contrast, overexpressing <i>MdARF6-4</i> led to reduced root development but increased root phosphorus content under <i>Gm</i> fungi inoculation, suggesting that MdARF6-4 is involved in <i>Gm</i>-mediated phosphorus absorption and root development. Moreover, both <i>MdPHR2</i> and <i>MdARF6-4</i> directly bound to the promoter area of the downstream phosphorus transporter <i>MdPHT1;13</i>, and these two transcription factors interacted with each other <i>in vivo</i> and <i>in vitro</i>. In summary, our study demonstrates that the interaction between MdPHR2 and MdARF6-4 synergistically regulates the <i>Gm</i> symbiosis and the transcription of <i>MdPHT1;13</i>, thereby promoting phosphorus absorption in apple rootstocks.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The switch-liker's guide to plant synthetic gene circuits","authors":"James P. B. Lloyd,&nbsp;Adil Khan,&nbsp;Ryan Lister","doi":"10.1111/tpj.70090","DOIUrl":"https://doi.org/10.1111/tpj.70090","url":null,"abstract":"<p>Synthetic gene circuits offer powerful new approaches for engineering plant traits by enabling precise control over gene expression through programmable logical operations. Unlike simple ‘always-on’ transgenes, circuits can integrate multiple input signals to achieve sophisticated spatiotemporal regulation of target genes while minimising interference with host cellular processes. Recent advances have demonstrated several platforms for building plant gene circuits, including systems based on bacterial transcription factors, site-specific recombinases and CRISPR/Cas components. These diverse molecular tools allow the construction of circuits that perform Boolean logic operations to control transgene expression or modulate endogenous pathways. However, implementing synthetic gene circuits in plants faces unique challenges, including long generation times that slow design-build-test cycles, limited availability of characterised genetic parts across species and technical hurdles in stable transformation. This review examines the core principles and components of plant synthetic gene circuits, including sensors, integrators, and actuators. We discuss recent technological developments, key challenges in circuit design and implementation, and strategies to overcome them. Finally, we explore the future applications of synthetic gene circuits in agriculture and basic research, from engineering stress resistance to enabling controlled bioproduction of valuable compounds. As this technology matures, synthetic gene circuits have the potential to enable sophisticated new plant traits that respond dynamically to environmental and developmental cues.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat-responsive MaHSF11 transcriptional activator positively regulates flavonol biosynthesis and flavonoid B-ring hydroxylation in banana (Musa acuminata)","authors":"Jogindra Naik,&nbsp;Ruchika Rajput,&nbsp;Samar Singh,&nbsp;Ralf Stracke,&nbsp;Ashutosh Pandey","doi":"10.1111/tpj.70084","DOIUrl":"https://doi.org/10.1111/tpj.70084","url":null,"abstract":"<div>\u0000 \u0000 <p>Plant flavonols act primarily as ultraviolet radiation absorbers, reactive oxygen species scavengers, and phytoalexins, and they contribute to biotic and abiotic stress tolerance in plants. Banana (<i>Musa acuminata</i>), an herbaceous monocot and important fruit crop, accumulates flavonol derivatives in different organs, including the edible fruit pulp. Although flavonol content varies greatly in different organs, the molecular mechanisms involving transcriptional regulation of flavonol synthesis in banana are not known. Here, we characterized three SG7-R2R3 MYB transcription factors (MaMYBFA1, MaMYBFA2, and MaMYBFA3) and heat shock transcription factor (MaHSF11), to elucidate the molecular mechanism involved in transcriptional regulation of flavonol biosynthesis in banana. MaMYBFA positively regulates <i>flavonol synthase</i> 2 (<i>MaFLS2</i>) and downregulates <i>MaFLS1</i>. We show these transcription factors to be weak regulators of flavonol synthesis. Overexpression of <i>MaHSF11</i> enhances flavonol contents, particularly that of myricetin, and promotes flavonol B-ring hydroxylation, which contributes to the diversity of flavonol derivatives. MaHSF11 directly interacts with the <i>MaFLS1</i> and <i>flavonoid 3′</i>,<i>5′-hydroxylase</i>1 (<i>MaF3′5′H1</i>) promoters, both <i>in vitro</i> and <i>in vivo</i>. MaHSF11 activates the expression of <i>MaDREB1</i> directly, which is known to promote cold and chilling tolerance in banana fruit. Overall, our study elucidates a regulatory mechanism for flavonol synthesis in banana and suggests possible targets for genetic optimization to enhance nutritional value and stress responses in this globally important fruit crop.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutagenesis of AcSQBP9 in kiwifruit results in reduction of malate via alteration of the expression of a plastidial malate dehydrogenase","authors":"Tong-hui Qi,&nbsp;Yu-qing Huang,&nbsp;Jia-hui Deng,&nbsp;Bei-ling Fu,&nbsp;Xiang Li,&nbsp;Shao-jia Li,&nbsp;Andrew C. Allan,&nbsp;Xue-ren Yin","doi":"10.1111/tpj.70082","DOIUrl":"https://doi.org/10.1111/tpj.70082","url":null,"abstract":"<div>\u0000 \u0000 <p>Organic acids are major contributors to the flavor of fleshy fruits. In kiwifruit, the Al-ACTIVATED MALATE TRANSPORTER gene (<i>AcALMT1</i>) is key to the accumulation of citrate, while factors driving malate metabolism remain largely unknown. During kiwifruit (<i>Actinidia chinensis</i> cv “Hongyang”) development, a rapid decline of malate content was observed between 6 and 12 weeks after full bloom (WAFB), which was studied using RNA-seq analysis. Co-expression network analysis indicated that expression of the chloroplast localized <i>AcPNAD-MDH1</i> (Plastid-Localized NAD-Dependent Malate Dehydrogenase) negatively correlated with malate content. Overexpression of <i>AcPNAD-MDH1</i> in kiwifruit resulted lower malate and citrate content in leaves. Among 15 transcription factors that are highly correlated with the expression of <i>AcPNAD-MDH1</i>, AcSQBP9 (SQUAMOSA PROMOTER-BINDING PROTEIN) was shown to directly bind the promoter of <i>AcPNAD-MDH1</i> to repress transcriptional activity. Moreover, targeted CRISPR-Cas9-induced mutagenesis of <i>AcSQBP9</i> in kiwifruit produced a significant decrease in malate and citrate, accompanied by an increase in <i>AcPNAD-MDH1</i> expression. Both PNAD-MDH and SQBP have not been widely studied in fruit metabolism, so the present omics-oriented study provides insights for both kiwifruit and general plant organic acid metabolism.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytophthora RxLR effector Pi18609 suppresses host immunity by promoting turnover of a positive immune regulator StBBX27","authors":"Qingguo Sun,&nbsp;Mingshuo Fan,&nbsp;Huishan Qiu,&nbsp;Yingtao Zuo,&nbsp;Tianyu Lin,&nbsp;Meng Xu,&nbsp;Jiahui Nie,&nbsp;Jiahui Wu,&nbsp;Jing Zhou,&nbsp;Ruimin Yu,&nbsp;Lang Liu,&nbsp;Zhendong Tian","doi":"10.1111/tpj.70074","DOIUrl":"https://doi.org/10.1111/tpj.70074","url":null,"abstract":"<div>\u0000 \u0000 <p>Oomycete pathogens deliver a number of RxLR effector proteins into host plant cells to manipulate immunity by targeting diverse host proteins. Here, we reveal that a <i>Phytophthora infestans</i> RxLR effector Pi18609 targets a potato B-box transcription factor (BBX), StBBX27, which was identified to positively regulate late blight resistance. Silencing the ortholog <i>NbBBX27</i> in <i>Nicotiana benthamiana</i> or RNA interfering <i>StBBX27</i> in potato increases host colonization by <i>P. infestans</i>, whereas transient expression of <i>StBBX27</i> in <i>N. benthamiana</i> or stable overexpression of <i>StBBX27</i> in potato enhances late blight resistance. Overexpression of StBBX27 in potato activates plant immune responses including upregulation of defense-related genes such as <i>StWRKY8</i>, <i>StOSML13</i>, <i>StCHTB4</i>, and <i>StSERK2</i>, and burst of reactive oxygen species (ROS). In addition, StBBX27 directly binds to the G-box of the <i>WRKY8</i> and <i>SERK2</i> promoters and activates their expression, which could be suppressed by Pi18609. Furthermore, we revealed that a ubiquitin E3 ligase COP1 promotes StBBX27 turnover by a proteasome-mediated system. Moreover, Pi18609 promotes the degradation of StBBX27 mediated by COP1. Collectively, this study reveals that StBBX27 positively regulates potato immunity and that is suppressed by effector Pi18609 to promote its turnover. This research extends our knowledge on BBXs function and the mechanisms behind <i>P. infestans</i> effectors suppression of host immunity.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}