The Plant Journal最新文献

{"title":"Effects of light on chloroplast translation in Marchantia polymorpha are similar to those in angiosperms and are not influenced by light-independent chlorophyll synthesis","authors":"Prakitchai Chotewutmontri,&nbsp;Rosalind Williams-Carrier,&nbsp;Susan Belcher,&nbsp;Alice Barkan","doi":"10.1111/tpj.70458","DOIUrl":"https://doi.org/10.1111/tpj.70458","url":null,"abstract":"<p>Translation of the chloroplast <i>psbA</i> mRNA in angiosperms is activated by photodamage of its gene product, the D1 subunit of photosystem II (PSII), providing nascent D1 for PSII repair. The involvement of chlorophyll in the regulatory mechanism has been suggested due to the regulatory roles of proteins proposed to mediate chlorophyll/D1 transactions and the fact that chlorophyll is synthesized only in the light in angiosperms. We used ribosome profiling and RNA-seq to address whether the effects of light on chloroplast translation are conserved in the liverwort Marchantia (<i>Marchantia polymorpha</i>), which synthesizes chlorophyll in both the dark and the light. As in angiosperms, ribosome occupancy on <i>psbA</i> mRNA decreased rapidly upon shifting plants to the dark and was rapidly restored upon a transfer back to the light, whereas ribosome occupancy on other chloroplast mRNAs changed very little. The results were similar in a <i>Marchantia</i> mutant unable to synthesize chlorophyll in the dark. Those results, in conjunction with pulse-labeling data, suggest that light elicits a plastome-wide activation of translation elongation and a specific increase in <i>psbA</i> translation initiation in <i>Marchantia</i>, as in angiosperms. These findings show that light regulates chloroplast translation similarly in vascular and non-vascular plants, and that constitutive chlorophyll synthesis does not affect light-regulated <i>psbA</i> translation initiation. Additionally, the translational outputs of chloroplast genes are similar in <i>Marchantia</i> and angiosperms but result from differing contributions of mRNA abundance and translational efficiencies. This adds to the evidence that chloroplast mRNA abundance and translational efficiencies co-evolve under selection to maintain protein outputs.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70458","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential regulation of calcium-activated plant kinases in Arabidopsis thaliana","authors":"Martin Zauser,&nbsp;Anja Liese,&nbsp;Anna Feldman-Salit,&nbsp;Melanie Krebs,&nbsp;Karin Schumacher,&nbsp;Tina Romeis,&nbsp;Ursula Kummer,&nbsp;Jürgen Pahle","doi":"10.1111/tpj.70413","DOIUrl":"https://doi.org/10.1111/tpj.70413","url":null,"abstract":"<p>The decoding of calcium signals by plant calcium-dependent kinases (CPKs) is not fully understood yet. Based on kinetic <i>in vitro</i> measurements of the activity of several CPK proteins, their individual activity profile was modeled and coupled to cytosolic calcium concentration changes from <i>in vivo</i> measurements of guard cells and epidermal leaf cells. In addition, computationally produced surrogate data were used. It was analyzed how individual activity profiles of CPKs will change in response to the different calcium time courses. In addition, the impact of modeling explicitly individual elementary reaction steps for binding and unbinding in comparison to using a heuristic Hill equation for the binding process was investigated.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70413","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tandem gene duplication facilitates intertidal adaptation in atypical mangrove plants","authors":"Yulong Li,&nbsp;Shao Shao,&nbsp;Ranran Zhu,&nbsp;Yarong Wang,&nbsp;Chuanfeng Jin,&nbsp;Min Liu,&nbsp;Kaichi Huang,&nbsp;Zixiao Guo,&nbsp;Ziwen He,&nbsp;Suhua Shi,&nbsp;Shaohua Xu","doi":"10.1111/tpj.70456","DOIUrl":"https://doi.org/10.1111/tpj.70456","url":null,"abstract":"<div>\u0000 \u0000 <p>Mangrove plants, originating from inland ancestors, have independently adapted to extreme intertidal zones characterized by salt and hypoxia stress. While typical mangroves exhibit specialized phenotypes, like viviparous seeds and salt secretion, atypical clades that have thrived without such traits are particularly suitable for exploring the molecular and physiological basis underlying plant adaptation to intertidal zones. We assembled a chromosome-level genome of an atypical mangrove, <i>Scyphiphora hydrophylacea</i>, the only mangrove species in Gentianales. Similar to other mangroves, <i>S. hydrophylacea</i> colonized intertidal zones during climatic optimum periods of sea-level rise. Despite lacking recent whole-genome duplications (WGDs), its genome acquired extensive tandem gene duplications (TDs), leading to the rapid expansion of key salt- and hypoxia-related genes. Transcriptome data further corroborated that TD-driven gene expansions contribute to stress tolerance. Specifically, the expansion of genes involved in cation transmembrane transport, osmotic regulation, and oxidative stress response may enhance salinity tolerance, and the expansion of signal transduction and energy metabolism genes in hypoxia-response pathways may confer waterlogging tolerance. Therefore, in the absence of large-scale gene duplication, the rapid expansion of core genes involved in salt and hypoxia tolerance through tandem duplication may represent a key force driving the adaptation of atypical mangroves. These findings also provide valuable insights for crop improvement strategies aimed at enhancing environmental resilience while maintaining phenotypic stability.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The RNA-binding protein OsDMCK1 is required for meiotic cytokinesis by regulating cell plate formation in rice","authors":"Ming Fu,&nbsp;Yuesheng Tian,&nbsp;Di Wu,&nbsp;Feiyang Xue,&nbsp;Hongbo Gao,&nbsp;Wanqi Liang","doi":"10.1111/tpj.70448","DOIUrl":"https://doi.org/10.1111/tpj.70448","url":null,"abstract":"<div>\u0000 \u0000 <p>Plant cytokinesis is distinguished from animal cytokinesis by the formation of a cell plate between dividing cells. While meiotic cytokinesis involves two successive nuclear divisions with distinct regulatory mechanisms from mitosis, the underlying mechanisms remain poorly understood. In this study, we identified OsDMCK1, a novel rice RNA-binding protein essential for male fertility. The <i>osdmck1-1</i> mutants exhibit defective meiotic cytokinesis, manifested by misoriented spindle orientation during meiosis II and disrupted callose deposition at both the cell plate and the outer wall of pollen mother cells (PMCs). Transcriptome analysis revealed significant downregulation of cytokinesis-related genes in <i>osdmck1-1</i> PMCs, including regulators of microtubule organization (<i>MAP65-3.2</i>, <i>BUB3.2</i>, <i>CDC20.3</i>, <i>TPX2</i>) and callose synthase genes (<i>GSL2</i>, <i>GSL5</i>). Importantly, RNA immunoprecipitation assays confirmed these genes as direct mRNA targets of OsDMCK1. Taken together, our study establishes OsDMCK1 as a crucial regulator of meiosis progression and reveals a novel post-transcriptional regulatory mechanism controlling rice meiotic cytokinesis.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering and comparison of cas12a-based genome editing systems in plants","authors":"Martin Bircheneder,&nbsp;Martin Parniske","doi":"10.1111/tpj.70410","DOIUrl":"https://doi.org/10.1111/tpj.70410","url":null,"abstract":"<p>While Cas9 and Cas12a are both RNA-guided endonucleases used for genome editing, only Cas12a is able to process pre-crRNA via its additional ribonuclease activity. This feature reduces the complexity of Cas12a versus Cas9-based genome editing systems thus providing an attractive alternative for generating site-specific mutations in plants. Here we aimed to improve the efficiency of the <i>cas12a</i>-based generation of two double-strand breaks flanking the open reading frame of a target gene, leading to its full deletion. To this end, we compared the relative impact of different components on <i>cas12a</i>-based gene deletion efficiency in three different eudicotyledons, <i>Arabidopsis thaliana</i>, <i>Lotus japonicus</i>, and <i>Nicotiana benthamiana</i>. We detected the highest <i>cas12a</i>-based editing efficiency with a combination of suitable promoters for crRNA and <i>cas12a</i> expression, a tandem terminator to control <i>cas12a</i> expression, a re-coded <i>cas12a</i>, adapted to the codon usage of <i>Arabidopsis</i> and engineered to carry introns, and encoding a Cas12a flanked by a nuclear localization signal at both ends. Our work revealed the high potential for improving <i>cas12a</i>-based genome editing systems for plant genetic research.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70410","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144929683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of NLG14 provides novel insights into the synergistic improvement of grain length, grain quality and salt tolerance in rice","authors":"Lei He,&nbsp;Lihua Zhuang,&nbsp;Tao Chen,&nbsp;Kai Lu,&nbsp;Wenhua Liang,&nbsp;Ling Zhao,&nbsp;Chunfang Zhao,&nbsp;Cheng Li,&nbsp;Qingyong Zhao,&nbsp;Zhen Zhu,&nbsp;Cailin Wang,&nbsp;Yadong Zhang","doi":"10.1111/tpj.70455","DOIUrl":"https://doi.org/10.1111/tpj.70455","url":null,"abstract":"<div>\u0000 \u0000 <p>Rice, as a vital food crop, faces persistent challenges in breeding programs aimed at achieving stable high yield under environmental stresses due to intrinsic trade-off mechanisms. This study functionally characterizes <i>NARROW AND LONGER GRAIN 14 (NLG14)</i>, which encodes a spermine synthase. Loss-of-function <i>nlg14</i> mutants exhibit slender grains due to enhanced cell expansion and proliferation, alongside significantly improved grain quality—manifested as reduced chalkiness, lower amylose/protein content, higher gel consistency, and superior taste value. These improvements correlate with decreased reactive oxygen species (ROS) accumulation and programmed cell death (PCD) in developing endosperm. Crucially, <i>nlg14</i> confers enhanced salt tolerance by elevating the K⁺/Na⁺ ratio and antioxidant enzyme activities. Mechanistically, disrupted spermine biosynthesis in <i>nlg14</i> redirects metabolic flux toward ethylene synthesis, activating ethylene signaling to enhance ROS scavenging and ion homeostasis. Furthermore, the transcription factor OsMYB2 directly binds to the promoter of <i>NLG14</i> and represses its expression via the abscisic acid (ABA) pathway. Haplotype analysis identifies natural <i>NLG14</i> variants (Class A) associated with longer grains, improved quality, and higher salt tolerance, demonstrating breeding potential. Collectively, NLG14 integrates grain morphology, quality, and stress adaptation through polyamine-ethylene-ABA crosstalk. Our results provide useful gene and germplasm resources for rice molecular breeding and shed insights for understanding yield and salt tolerance trade-off mechanisms.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144929685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transposable elements drive evolution and perturb gene expression in Brassica rapa and B. oleracea","authors":"Po-Xing Zheng,&nbsp;Chia-Ying Ko,&nbsp;Jheng-Yan Ou,&nbsp;Andrea Zuccolo,&nbsp;Yao-Cheng Lin","doi":"10.1111/tpj.70452","DOIUrl":"https://doi.org/10.1111/tpj.70452","url":null,"abstract":"<p>Transposable elements (TEs) significantly influence genomic diversity and gene regulation in plants. <i>Brassica rapa</i> and <i>B. oleracea</i>, with their distinct domestication histories, offer excellent models to explore TE dynamics. Here, we developed a refined TE classification method and systematically analyzed TEs across 12 <i>B. rapa</i> and <i>B. oleracea</i> genomes, identifying 1878 TE families. Approximately half (49.5%) of these TE families were shared between the two species, reflecting a common evolutionary origin, whereas species-specific expansions, particularly among long-terminal repeat (LTR) retrotransposons, underscore their roles in genomic differentiation. We notably characterized a heat-responsive Ty1-copia family (Copia0035) in <i>B. oleracea</i> roots, distinguished by low GC content and the absence of CG and CHG methylation motifs, sharing regulatory similarities with the <i>Arabidopsis</i> heat-induced ONSEN element. Syntenic analyses of gene-TE associations highlighted significant intraspecies TE insertion variability, with more accession-specific insertions in <i>B. rapa</i> and more conserved insertions, often associated with distinct morphotypes in <i>B. oleracea</i>. Gene ontology enrichment indicated TE involvement in developmental, reproductive, and stress response pathways. Transcriptome analysis across diverse accessions revealed that genes proximal to TEs, particularly those regulating floral development and flowering time, exhibit increased expression variability. These findings advance our understanding of TE-mediated genome evolution in <i>Brassica</i> species and underscore their potential utility in breeding and genome engineering strategies for crop improvement.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70452","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144927575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plants maximise chloride uptake during early vegetative development to stimulate cell expansion, maturation of the photosynthetic apparatus, and growth","authors":"Procopio Peinado-Torrubia,&nbsp;Juan D. Franco-Navarro,&nbsp;Marta Lucas,&nbsp;David Romero-Jiménez,&nbsp;Francisco J. Moreno-Racero,&nbsp;Pablo Díaz-Rueda,&nbsp;Miguel A. Rosales,&nbsp;Marika Lindahl,&nbsp;Antonio Díaz-Espejo,&nbsp;Rosario Álvarez,&nbsp;José Manuel Colmenero-Flores","doi":"10.1111/tpj.70378","DOIUrl":"https://doi.org/10.1111/tpj.70378","url":null,"abstract":"<p>Despite being an essential micronutrient and its recent classification as a beneficial macronutrient, chloride (Cl⁻) has traditionally been considered of limited agricultural relevance and a potentially toxic saline ion. This study provides the first comprehensive demonstration of the quantitative and qualitative importance of Cl⁻ during early vegetative development (EVD) of tobacco and <i>Arabidopsis thaliana</i> plants. During this developmental stage, these and other species (including celery, lettuce, Swiss chard, spinach, squash, tomato, chili pepper, eggplant, and perennial ryegrass) exhibit the highest demand and transport rate of this non-assimilable mineral nutrient to maximise growth of these herbaceous and also woody (such as citrus and olive) species. While Cl⁻ promotes cell expansion across all growth stages, its particularly pronounced stimulation of plant growth during EVD is associated with enhanced photosynthetic performance and PSII activity. This enhancement is in turn linked to a reduction in non-regulated energy dissipation in PSII and an increase in the electron transport rate, along with ultrastructural changes in chloroplasts, underscoring that Cl⁻ is specifically required during EVD to drive the maturation of the photosynthetic apparatus. Unlike adult plants, the growth deficiencies caused by sub-macronutrient Cl⁻ levels during EVD cannot be mitigated by equivalent nitrate (NO₃⁻) supplementation. As EVD concludes, plant demand for Cl⁻ gradually decreases, accompanied by a reduced growth response to Cl⁻ and an increased reliance on NO₃⁻, emphasising stage-specific nutrient needs. The relevance of Cl⁻ as a morphogenic driver during a critical stage of development has significant implications for optimizing agronomic practices, particularly by reducing dependence on nitrogen fertilisers.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70378","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NaCl promotes tomato fruit coloring by relieving SlSR3-induced transcriptional inhibition of lycopene synthesis-related genes","authors":"Xuemei Hou,&nbsp;Tong Wang,&nbsp;Yihua Li,&nbsp;Aiyin Cui,&nbsp;Yuanyuan Kong,&nbsp;Yali Zhu,&nbsp;Hua Fang,&nbsp;Chunlei Wang,&nbsp;Weibiao Liao","doi":"10.1111/tpj.70450","DOIUrl":"https://doi.org/10.1111/tpj.70450","url":null,"abstract":"<div>\u0000 \u0000 <p>Although salt stress has an adverse effect on plant growth and development, mild salt stress acts as an elicitor of biosynthesis and thus improves fruit quality. To date, the role and mechanism of NaCl in accelerating tomato (<i>Solanum lycopersicum</i>) fruit coloring remain unclear. This study found that 50 mM NaCl treatment (moderate salt stress) reduced the chlorophyll content, increased the carotenoid and lycopene content, and accelerated tomato color transition without decreasing yield. Moreover, NaCl treatment downregulated <i>calmodulin-binding transcription activator</i> (<i>CAMTA5</i>)/<i>signal responsive</i> (<i>SR3</i>). Knockout of <i>SlSR3</i> by CRISPR/Cas 9 (<i>sr3</i> mutant) accelerated chlorophyll degradation and carotenoid and lycopene accumulation and upregulated chlorophyll degradation (<i>PPH</i>) and lycopene synthesis (<i>PSY2</i>, <i>PDS</i>, and <i>ZDS</i>) genes in tomato fruit, thereby accelerating tomato coloring. However, <i>SlSR3</i> overexpression had the opposite effect. Although NaCl treatment decreased chlorophyll, increased carotenoids, and upregulated <i>PPH</i>, <i>PSY2</i>, <i>PDS</i>, and <i>ZDS</i> in wild type and OE-<i>sr3</i> fruit, these changes were not observed in <i>sr3</i> mutant fruit. Therefore, <i>PPH</i>, <i>PSY2</i>, <i>PDS</i>, and <i>ZDS</i> might be involved in SR3-regulated coloring under moderate salt stress. Further results showed that SlSR3 could directly bind to the promoter of <i>PSY2</i> and <i>ZDS</i> via the CG-1 domain, thereby downregulating <i>PSY2</i> and <i>ZDS</i>. However, NaCl treatment reversed the transcriptional inhibition of SlSR3 on <i>PSY2</i> and <i>ZDS</i> expression, thereby upregulating <i>PSY2</i> and <i>ZDS</i>. Collectively, our results suggest that the promoting effects of NaCl on fruit coloring may be dependent on SlSR3-induced transcriptional regulation of lycopene synthesis-related genes <i>PSY2</i> and <i>ZDS</i>. Therefore, our study provides a better understanding of the function of SlSR3 in tomato coloring and offers insights on the molecular mechanism underlying the effects of moderate salt stress on tomato color transformation.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha","authors":"Hee Sung Kang,&nbsp;Xin Tong,&nbsp;Alban Mariette,&nbsp;Minnie Leong,&nbsp;Cherie Beahan,&nbsp;Eduardo Flores-Sandoval,&nbsp;Gustav B. Pedersen,&nbsp;Carsten Rautengarten,&nbsp;John L. Bowman,&nbsp;Berit Ebert,&nbsp;Anthony Bacic,&nbsp;Monika S. Doblin,&nbsp;Staffan Persson,&nbsp;Edwin R. Lampugnani","doi":"10.1111/tpj.70439","DOIUrl":"https://doi.org/10.1111/tpj.70439","url":null,"abstract":"<p>The liverwort <i>Marchantia polymorpha</i> has emerged as an important plant model for developmental studies and may become central to elucidate the complex process of cell wall polysaccharide biosynthesis. This study comprehensively analyses the composition and structure of cell wall glycans across eight different <i>M. polymorpha</i> tissue types. We show that while the cell walls largely mirror known land plant cell wall composition, they also exhibit some unique characteristics. For example, <i>M. polymorpha</i> cell walls displayed a remarkably low overall pectin content, yet the relative abundance of pectic α-(1,5)-arabinan in sporophytes hints at its putative role in the evolution and complexity of spermatophyte cell walls. Furthermore, through comparative analyses of glycosyltransferase (GT) families across plant species, we found that while <i>M. polymorpha</i> generally has low genetic redundancy in most cell wall-related GT families, it also exhibits a diversified GT repertoire in four families, indicating uniqueness in certain cell wall biosynthesis pathways. To support research underpinning cell wall biosynthesis, we developed a Gateway compatible compendium of 87 <i>M. polymorpha</i> GTs, providing a valuable resource for genetic and functional studies. Our study thus works as a foundation to drive new insights into cell wall evolution, structure and function across the plant kingdom.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 5","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70439","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}