The Plant Journal最新文献

{"title":"Correction to “Insights into the regulatory roles of LIKE-HETEROCHROMATIN PROTEIN 1 and its targeting to different nuclear compartments modulated by NLS and the conserved domains in the moss Physcomitrium patens”","authors":"","doi":"10.1111/tpj.71115","DOIUrl":"https://doi.org/10.1111/tpj.71115","url":null,"abstract":"<p>\u0000 <span>Goel, B.</span>, <span>Kapoor, S.</span> &amp; <span>Kapoor, M.</span> (<span>2026</span>) <span>Insights into the regulatory roles of LIKE-HETEROCHROMATIN PROTEIN 1 and its targeting to different nuclear compartments modulated by NLS and the conserved domains in the moss <i>Physcomitrium patens</i></span>. <i>The Plant Journal</i>, <span>127</span>, e71032. Available from: https://doi.org/10.1111/tpj.71032.</p><p>In the first paragraph of the “Chromatin immunoprecipitation (ChIP) and ChIP-PCR” subsection of the “Material and Methods” section, the unit “ml” was incorrectly used instead of “μl” in the following instances:</p><p>The text “the nuclear pellet was resuspended in 300 ml” was incorrect and should be “the nuclear pellet was resuspended in 300 μl.”</p><p>The text “This was carefully layered onto 300 ml” was incorrect and should be “This was carefully layered onto 300 μl.”</p><p>The text “discarded and to the pellet 300 ml” was incorrect and should be “discarded and to the pellet 300 μl.”</p><p>The text “sample was checked on 2% agarose gel and 10 ml” was incorrect and should be “sample was checked on 2% agarose gel and 10 μl.”</p><p>The text “Next day, 50 ml magnetic Dynabeads” was incorrect and should be “Next day, 50 μl magnetic Dynabeads.”</p><p>The text “Finally, 250 ml Elution buffer” was incorrect and should be “Finally, 250 μl Elution buffer.”</p><p>The text “Crosslinking was reversed by adding 20 ml 5 M NaCl” was incorrect and should be “Crosslinking was reversed by adding 20 μl 5 M NaCl.”</p><p>The text “To the input samples up to 500 ml elution buffer and 20 ml 5 M NaCl was added.” was incorrect and should be “To the input samples, up to 500 μl elution buffer and 20 μl 5 M NaCl was added.”</p><p>The text “For DNA purification, 10 ml 0.5 M EDTA, 20 ml Tris–HCl (pH 6.5), and 2 ml Proteinase K”, was incorrect and should be “For DNA purification, 10 μl 0.5 M EDTA, 20 μl Tris–HCl (pH 6.5), and 2 μl Proteinase K.”</p><p>The text “1 ml Glycogen carrier” was incorrect and should be “1 μl Glycogen carrier.”</p><p>We apologize for this error.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148903940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pathogenic effector PHYL1JWB interacts with ZjCURT1A to mediate jujube witches' broom-induced photosynthesis impairment of jujube trees","authors":"Fangfang Huang,&nbsp;Changfeng Ai,&nbsp;Chong Yang,&nbsp;Jie Feng,&nbsp;Meng Yang,&nbsp;Li Dai,&nbsp;Chaoling Xue,&nbsp;Hongtai Li,&nbsp;Jiaxue Cui,&nbsp;Xiangjie Qu,&nbsp;Jin Zhao,&nbsp;Mengjun Liu,&nbsp;Xuan Zhao,&nbsp;Zhiguo Liu","doi":"10.1111/tpj.71104","DOIUrl":"https://doi.org/10.1111/tpj.71104","url":null,"abstract":"<p>Photosynthesis impairment and the accompanying leaf yellowing represent a prominent and physiologically critical symptom of phytoplasma infection. However, the underlying molecular mechanisms remain poorly understood. In this study, we identified the Jujube Witches' Broom pathogenic effector PHYL1_JWB, which was previously reported to induce phyllody in jujube, as a key inducer of photosynthesis impairment. Overexpression of <i>PHYL1</i>_<i>JWB</i> in sour jujube and <i>Arabidopsis thaliana</i> disrupted photosynthesis by causing severe disorganization of chloroplast ultrastructure. PHYL1_JWB directly interacts with the integral thylakoid membrane protein ZjCURT1A and promotes its 26S proteasome-dependent degradation independently of lysine ubiquitination, leading to disruption of thylakoid structure and photosynthetic dysfunction. The conserved two α-helices of PHYL1_JWB and the C-terminal segment of ZjCURT1A containing the thylakoid membrane curvature-related CAAD motif are essential for their interaction. Together, these findings reveal that PHYL1_JWB impairs photosynthesis by disrupting chloroplast ultrastructural in addition to inducing phyllody, thereby contributing to abnormal plant growth. This work highlights the functional pleiotropy of pathogenic effectors and underscores the need to consider their impacts on multiple physiological processes in the context of phytoplasma pathogenicity.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148903937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"JanusX: an integrated and high-performance platform for scalable genome-wide association studies and genomic selection.","authors":"Jingxian Fu, Anqiang Jia, Haiyang Wang, Hai-Jun Liu","doi":"10.1111/tpj.71105","DOIUrl":"10.1111/tpj.71105","url":null,"abstract":"<p><p>As genomic datasets expand in both sample size and marker density, genome-wide association studies (GWAS) and genomic selection (GS) require workflows that remain statistically rigorous, computationally efficient, and reproducible across the full analysis path, from genotype matrix to decision-relevant outputs. Here we present JanusX, an integrated high-performance framework that provides a streamlined, user-oriented workflow for GWAS and GS by unifying data handling, model execution, and visualization. Across simulated and real datasets, JanusX maintained high concordance with established baselines while substantially reducing runtime and memory usage. In GWAS, JanusX achieved up to a 19-fold speedup over GEMMA in linear mixed model (LMM) inference, and additionally implemented LMM inference based on a sparse genomic relationship matrix with GRAMMAR-Gamma calibration, alleviating computational and memory bottlenecks in large-scale cohorts. JanusX also provides a FarmCPU implementation within its GWAS module, achieving a median 11.4-fold runtime improvement and reducing peak memory usage by 84.9% relative to rMVP. In GS, JanusX integrates an optimized best linear unbiased prediction (BLUP) backend that adaptively selects sample- and single-nucleotide polymorphism (SNP)-space solvers and incorporates a Preconditioned Conjugate Gradient solver. This implementation efficiently completes five-fold cross-validation of 500k individuals × 500k SNPs in 35.1 min with only 14.3 gibibytes (GiB) of peak memory. Beyond BLUP, JanusX integrates Bayesian and machine-learning predictors under a single interface with compact automatic tuning to ensure robust cross-model performance. JanusX therefore enables efficient locus discovery and genomic prediction under consistent analytical assumptions, even in large-scale cohorts.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":"e71105"},"PeriodicalIF":6.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13578591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148975199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dim green light enables day-and-night monitoring of leaf movements.","authors":"Eva Herrero, Alison R Gill, Samalka Wijeweera, Daniel N Ginzburg, John D Stamford, Andreas Antoniades, Jennifer R Bromley, Jenny C Mortimer, Matthew Gilliham, A Harvey Millar, Alex A R Webb","doi":"10.1111/tpj.71119","DOIUrl":"10.1111/tpj.71119","url":null,"abstract":"<p><p>Understanding plant growth, movement and development in the aerial plant body requires imaging across day-and-night cycles to quantify temporal dynamics and rhythmicity. This requires imaging in light during the day and in darkness at night without perturbing plant physiology. Nighttime imaging has typically depended on infrared (IR) illumination, producing monochrome datasets that require specialised hardware and separate analysis pipelines when combined with daytime RGB imaging. Here, we evaluated very low-intensity green (dimG) illumination from standard LEDs as a practical alternative for colour-consistent nighttime imaging and assessed its physiological impact in Arabidopsis thaliana and Lactuca sativa (lettuce). We show that high-resolution colour images can be obtained under dimG using low-cost cameras, with sufficient consistency between full-spectrum and dimG images to allow direct comparison and unified image analysis. We show that very low-fluence green light (<0.5 mmol m^-2 s^-1) does not sustain circadian oscillations of gene activity under continuous exposure and does not perturb rhythms when applied during the dark phase of diel cycles. DimG imaging enabled accurate detection of diel leaf-movement profiles in Arabidopsis circadian mutants, revealing genotype-specific phase differences under varying photoperiods. In lettuce, dimG pulses and continuous dimG enabled accurate quantification of diel leaf movement without affecting growth, stomatal opening, electron transport rate or chlorophyll content. Motion profiles under continuous dimG mirrored those under darkness. Our findings establish dim green illumination as a cost-effective solution for nighttime imaging, simplifying phenotyping workflows with minimal impact on physiology.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":"e71119"},"PeriodicalIF":6.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13578868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148975237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Natural haplotype variation in OsGSK3 fine-tunes grain length via phosphorylating and stabilizing OsRanBP1 in rice\".","authors":"","doi":"10.1111/tpj.71126","DOIUrl":"10.1111/tpj.71126","url":null,"abstract":"","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 6","pages":"e71126"},"PeriodicalIF":6.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SDR3.1 negatively regulates seed dormancy in Poaceae crops.","authors":"Naihui Guo, Ruihu An, Mengxue Xu, Yuanyuan Li, Zongliang Ren, Jun Jiang, Bonian Cai, Xinzhu Huang, Shikai Hu, Gaoneng Shao, Guiai Jiao, Lihong Xie, Ling Wang, Fengli Zhao, Yujun Zhu, Shaoqing Tang, Zhonghua Sheng, Peisong Hu","doi":"10.1111/tpj.71141","DOIUrl":"10.1111/tpj.71141","url":null,"abstract":"<p><p>Elucidating the molecular mechanisms that govern seed dormancy is important for developing crops with improved resistance to preharvest sprouting (PHS). A series of seed dormancy genes have been cloned, but the connections among different seed dormancy genes remain poorly understood. Here, we have revealed the relationship between two important dormancy quantitative trait loci (QTLs): SDR3.1 and Sdr4. SDR3.1 formed complexes with ABI3 and ABI5 and suppressed their activation of Sdr4 transcription. Haplotype analysis showed that the germination rate of SDR3.1 L-type and Sdr4 n- or K-type combinations was low, and the parents of modern core breeding do not contain l-type. Analysis of transgenic plants confirmed that orthologs of SDR3.1 have conserved functions in controlling seed dormancy in rice (Oryza sativa L.), foxtail millet (Setaria italica (L.) P. Beauv.), and maize (Zea mays), but they have different molecular regulatory mechanisms. This study uncovers the OsSDR3.1-ABI3/ABI5-Sdr4 regulatory module conferring improved PHS resistance, while establishing an effective approach to characterize core functional genes for important agronomic traits across Poaceae species with rice as a model.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 6","pages":"e71141"},"PeriodicalIF":6.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13639105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149101753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of chloroplast macromolecular complex copy numbers and subunit stoichiometries during the Chlamydomonas reinhardtii cell cycle.","authors":"Stefan Schmollinger, Daniela Strenkert, Samuel O Purvine, Carrie D Nicora, Eric Soubeyrand, Gilles J Basset, Sabeeha S Merchant","doi":"10.1111/tpj.71128","DOIUrl":"10.1111/tpj.71128","url":null,"abstract":"<p><p>An unbiased, quantitative view of biomolecules in a living cell is a prerequisite for accurate modeling approaches and informs our understanding of cellular metabolism at scale. In this work, we used the total protein approach (TPA), in which the total protein mass of a given proteomics sample is used as a calibrator for absolute protein quantification, to determine protein abundances during the Chlamydomonas reinhardtii diurnal cycle. We use external, independently measured quantitative markers (metals, pigments) to assess the absolute protein abundances in unlabeled whole cell extracts. We calculate protein abundances in fg cell^-1 of 7322 Chlamydomonas proteins, 2266 of which were captured in every time point, including the major proteins involved in the light reactions, photoprotection, proteostasis, and fatty acid metabolism during a cell cycle. As expected, Rubisco large and small subunits are present in a 1:1 stoichiometry, with the large subunit being the most abundant protein in our data set, averaging 5.05 × 10⁶ molecules per cell, reflecting 2.7% of the total protein mass. We noticed that PSII is the most abundant complex involved in the light reactions with 2.08 × 10⁶ complexes per cell. PSI averages 1.75 × 10⁶ complexes per cell and cytochrome b₆f averages 0.77 × 10⁶ complexes per cell. The TPA is a robust tool to study proteome dynamics quantitatively, while avoiding artifacts due to biochemical fractionation. Our proteome data set with an unprecedented temporal resolution is a valuable resource to assess protein abundances during the cell cycle in the reference alga Chlamydomonas.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":"e71128"},"PeriodicalIF":6.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13585458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription and phosphorylation of CATALASE2 contribute to cold stress tolerance in Hevea brasiliensis","authors":"Ru-Feng Song,&nbsp;Wei Wen,&nbsp;Lin Li,&nbsp;Huan Yang,&nbsp;Chaorong Tang,&nbsp;Wen-Cheng Liu,&nbsp;Hong-Mei Yuan","doi":"10.1111/tpj.71097","DOIUrl":"10.1111/tpj.71097","url":null,"abstract":"<p>Despite the economic importance of the rubber tree (<i>Hevea brasiliensis</i>) and its sensitivity to cold stress, the molecular mechanisms that govern its cold response are still largely unknown. Here, we identified <i>H. brasiliensis</i> CATALASE2 (HbCAT2) as a factor associated with cold tolerance in this species, likely through reducing excess H₂O₂. Cold stress significantly induced <i>HbCAT2</i> transcription and catalase activity in <i>H. brasiliensis</i>, and overexpression of <i>HbCAT2</i> in both <i>H. brasiliensis</i> and Arabidopsis enhanced cold tolerance and reduced H₂O₂ accumulation. Furthermore, we found that HbCBF1, a key transcription factor in plant cold signaling, could directly bind to and activate <i>HbCAT2</i>, and overexpression of <i>HbCAT2</i> was able to alleviate the cold hypersensitivity of the Arabidopsis <i>cbf123</i> triple mutant. Additionally, the protein kinase HbSnRK2.6A interacted with and phosphorylated HbCAT2 to stimulate its catalase activity. Genetic evidence showed that HbSnRK2.6A-mediated cold tolerance is associated with CAT2 function. Together, our findings are consistent with a dual regulatory module in which HbCBF1-mediated <i>HbCAT2</i> transcription and HbSnRK2.6A-mediated phosphorylation of HbCAT2 may contribute to enhancing cold tolerance by preventing H₂O₂ overaccumulation in <i>H. brasiliensis</i>.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148838516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural leaf shape variation reveals diverse transcriptional targets of GmJAG1 during soybean leaf development","authors":"Bishal G. Tamang,&nbsp;Casey Kramer,&nbsp;Elizabeth A. Ainsworth","doi":"10.1111/tpj.71088","DOIUrl":"10.1111/tpj.71088","url":null,"abstract":"<p>The <i>JAGGED</i> transcription factor family regulates lateral organ development across angiosperms. In soybean (<i>Glycine max</i> Merr.), a D9H mutation in the EAR repression motif of <i>GmJAG1</i> causes a narrow-leaflet phenotype and explains over 70% of phenotypic variance in leaf shape. Because this mutation does not affect the zinc finger DNA-binding domain, both alleles bind identical targets but differ in repressor recruitment. Previous studies mapped <i>GmJAG1</i> binding sites, but the functional targets controlling leaf morphology are uncharacterized. Here, we used comparative transcriptomics across four soybean genotypes with contrasting leaf shape, spanning a developmental time series from shoot apex to mature leaf, and identified 1567 putative candidate target genes. <i>GmJAG1</i> expression was confined to the shoot apex, yet 99.1% of candidate targets maintained differential expression throughout development. We found that neither Kip-Related Protein (<i>KRP</i>) cell cycle inhibitors nor Cyclin-Dependent Kinases (CDKs) showed differential expression despite binding evidence in Arabidopsis. However, D-type cyclins were upregulated in narrow-leaf genotypes suggesting that soybean <i>GmJAG1</i> acts through cyclin-mediated rather than <i>KRP</i>-mediated cell cycle regulation described in Arabidopsis– a divergence in regulatory logic between the two species. Pathway analysis revealed enrichment of auxin (1.8-fold, <i>P</i> = 0.02) and salicylic acid (fourfold, <i>P</i> = 0.016) genes among JAG1_D9H targets. Filtering by differential expression, binding data, phenotype correlation, and co-expression network membership identified 79 high-confidence targets, including orthologs of <i>NPH3</i> (phototropin-mediated leaf flattening), <i>MIK2</i> (cell wall integrity sensing), <i>RD22</i> (ABA-responsive stress signaling), and <i>SCL23</i> (GRAS transcription factor in bundle sheath development). These candidates provide targets for functional validation and breeding in legumes.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148823813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The root-knot nematode effector Mi1D08B targets a NINJA-family co-repressor to suppress jasmonate production and promote infection in soybean","authors":"Peitong Li,&nbsp;Sobhan Bahrami Zadegan,&nbsp;Nicole Coffey,&nbsp;Nandiny Ghosh,&nbsp;J. Hollis Rice,&nbsp;Ahmad El-Messidi,&nbsp;Mohamed Boshnag,&nbsp;Tabibul Islam,&nbsp;Tessa Burch-Smith,&nbsp;Vince Pantalone,&nbsp;Tarek Hewezi","doi":"10.1111/tpj.71095","DOIUrl":"https://doi.org/10.1111/tpj.71095","url":null,"abstract":"<p>Plant-parasitic nematodes deploy secreted effector proteins that reprogram host cellular processes to establish parasitism. Here, we characterized Mi1D08B, a putative dorsal-gland effector from the root-knot nematode <i>Meloidogyne incognita</i> and defined its role in subverting soybean jasmonate-mediated immunity. Mi1D08B is conserved across several <i>Meloidogyne</i> species and localizes to the plant nucleus and cytoplasm. Overexpression of <i>Mi1D08B</i> significantly increased galling and egg production, demonstrating a strong virulence role. A gall-specific yeast two-hybrid screen identified the NINJA-family co-repressor mc410 as a host target of Mi1D08B. Consistent with the functional relevance of this interaction, <i>mc410</i> promoter activity was detected in galls and giant cells throughout nematode infection. Genetic manipulation of <i>mc410</i> phenocopied Mi1D08B activity as <i>mc410</i> overexpression enhanced susceptibility, whereas <i>mc410</i> silencing reduced nematode infection. Hormone profiling revealed that <i>Mi1D08B</i> overexpression elevated 12-oxo-phytodienoic acid (OPDA) but reduced jasmonic acid (JA) and JA-Ile, consistent with a bottleneck at the peroxisomal OPDA-to-JA conversion. Furthermore, several metabolites associated with this conversion were reduced, indicating that Mi1D08B perturbs metabolic flux through the peroxisomal phase of jasmonate biosynthesis. Gene expression analyses supported this biochemical signature, with upregulation of plastidial OPDA-biosynthetic genes and downregulation of peroxisomal OPDA-reductases, JA-conjugating enzymes, and JA-responsive markers. Together, our data support a model in which Mi1D08B effector interacts with a NINJA co-repressor to suppress JA biosynthesis and signaling, thereby coupling nuclear transcriptional repression to altered metabolism. This mechanism deepens our understanding of nematode manipulation of host hormone networks and highlights the Mi1D08B–mc410 interface and OPDA conversion as promising targets for engineering nematode resistance.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}