The Plant Journal最新文献

{"title":"The chloroplast-located HKT transporter plays an important role in fertilization and development in Physcomitrium patens","authors":"Carolina Yanez-Dominguez,&nbsp;Karla Macedo-Osorio,&nbsp;Daniel Lagunas-Gomez,&nbsp;Diana Torres-Cifuentes,&nbsp;Juan Castillo-Gonzalez,&nbsp;Guadalupe Zavala,&nbsp;Omar Pantoja","doi":"10.1111/tpj.17253","DOIUrl":"https://doi.org/10.1111/tpj.17253","url":null,"abstract":"<div>\u0000 \u0000 <p>Cell survival depends on the maintenance of cell homeostasis that involves all the biochemical, genomic and transport processes that take place in all the organelles within a eukaryote cell. In particular, ion homeostasis is required to regulate the membrane potential and solute transport across all membranes, any alteration in these parameters will reflect in the malfunctioning of any organelle, and consequently, in the development of the organism. In plant cells, sodium transporters play a central role in keeping the concentrations of this cation across all membranes under physiological conditions to prevent its toxic effects. HKT transporters are a family of membrane proteins exclusively present in plants, with some homologs present in prokaryotes. HKT transporters have been associated to salt tolerance in plants, retrieving any leak of the cation into the xylem, or removing it from aerial parts, including the flowers, to be transported to the roots along the phloem. This function has been assigned as most of the HKT transporters are located at the plasma membrane. Here, we report the localization of the HKT from <i>Physcomitrium patens</i> to the thylakoid membrane, reminiscent of the prokaryote origin of these family of transporters. Mutation of <i>PpHKT</i> leads to several alterations in the phenotype of the organism, including the lack of sporophyte formation, and changes in expression of many genes. These alterations suggest that the breakdown in chloroplast ion homeostasis triggers a signalling cascade to the nucleus to communicate its status, being important for the moss to complete its life cycle.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The heat shock factor HSFB1 coordinates plant growth and drought tolerance in Arabidopsis","authors":"Lanjie Zheng,&nbsp;Qianlong Zhang,&nbsp;Chen Wang,&nbsp;Zhongbao Wang,&nbsp;Jie Gao,&nbsp;Runcong Zhang,&nbsp;Yong Shi,&nbsp;Xu Zheng","doi":"10.1111/tpj.17258","DOIUrl":"https://doi.org/10.1111/tpj.17258","url":null,"abstract":"<div>\u0000 \u0000 <p>Plants are constantly challenged by a diversity of abiotic stressors, and growth arrest is a common plant response aimed at enhancing stress tolerance. Because of this growth/stress tolerance antagonism, plants must finely modulate their growth and responses to environmental stimuli. Here, we demonstrate that HSFB1, a heat shock transcription factor, plays a critical role in the coordination of plant growth and drought stress responses in <i>Arabidopsis thaliana</i>. First, we found that HSFB1 negatively regulates plant growth and development under normal conditions and that <i>HSFB1</i> expression is enhanced under drought stress. Conversely, the loss-of-function mutant <i>hsfb1</i> exhibited increased plant growth and reduced drought stress tolerance compared with the wild-type. Consistently, overexpression of <i>HSFB1</i> suppressed plant growth and enhanced drought stress tolerance. Subsequently, via chromatin immunoprecipitation sequencing, RNA sequencing, and transient expression assays, we screened and identified the heat shock protein 101 (HSP101) gene as a direct transcriptional target of HSFB1. Genetic analysis suggested that <i>HSP101</i> functions downstream of HSFB1 to positively regulate drought tolerance in plants. Furthermore, we found that HSFB1 physically interacts with the eukaryotic translation initiation factor eIF3G1, and this interaction appears to be further enhanced under drought stress. Notably, the mutation of <i>eif3g1</i> increased the severity of drought-induced growth inhibition in the <i>hsfb1</i> mutant, and eIF3G1 enhanced the transcriptional activation of HSFB1 on the <i>HSP101</i> promoter under drought stress. Altogether, our findings highlight HSFB1 as a key regulator coordinating plant growth and drought stress responses in Arabidopsis.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic reprogramming underlying anatomical and physiological characteristics of poplar wood in acclimation to changing light and nitrogen availabilities","authors":"Yan Lu,&nbsp;Shurong Deng,&nbsp;Jiangting Wu,&nbsp;Hong Li,&nbsp;Jing Zhou,&nbsp;Wenguang Shi,&nbsp;Payam Fayyaz,&nbsp;Zhi-Bin Luo","doi":"10.1111/tpj.17234","DOIUrl":"https://doi.org/10.1111/tpj.17234","url":null,"abstract":"<div>\u0000 \u0000 <p>To explore the proteomic regulation that underlies the physiological, anatomical, and chemical characteristics of wood in acclimation to changing light and nitrogen (N), saplings of <i>Populus</i> × <i>canescens</i> were treated with control or high irradiance in combination with low, control or high N for 4 months. High irradiance led to elevated levels of starch, sucrose, glucose, and fructose, decreased concentrations of ammonium, nitrate, most amino acids and total N, wider xylem, more xylem cell layers, narrower vessel lumina, longer fiber cells, greater fiber wall thickness, and more cellulose and hemicellulose but less lignin deposition in poplar wood. Limiting N resulted in increased levels of starch and sucrose, reduced levels of glucose, fructose, ammonium, nitrate, amino acids and total N, narrower xylem, fewer xylem cell layers, reduced vessel lumen diameter, thicker fiber walls, and less cellulose and more hemicellulose and lignin accumulation, whereas high N had the opposite effects on poplar wood. Correspondingly, numerous differentially abundant proteins, which are related mainly to the metabolism of carbohydrates and amino acids, cell division and expansion, and deposition of secondary cell walls, such as sucrose synthase 6 (SUS6), cell division cycle protein 48 (CDC48) and laccases (LACs), were identified in poplar cambiums in response to changes in light intensity and N availability. These results suggest that proteomic relays play essential roles in regulating the physiological characteristics and anatomical and chemical properties of poplar wood in acclimation to changing light and N availabilities.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lysine acetylation modulates drought stress responses in birch (Betula platyphylla) through metabolic and transcriptional pathway regulation","authors":"Shilin Sun,&nbsp;Xue Han,&nbsp;Jiayi Wang,&nbsp;Mingshuang Liu,&nbsp;Siqi Wu,&nbsp;Di Wu,&nbsp;Yucheng Wang","doi":"10.1111/tpj.17260","DOIUrl":"https://doi.org/10.1111/tpj.17260","url":null,"abstract":"<div>\u0000 \u0000 <p>Acetylation modifies protein subcellular localization, stability, enzymatic activity, and protein–protein and protein-DNA interactions, playing a crucial role in mediating protein function. However, research on non-histone acetylation remains limited. This study investigates changes in lysine acetylation (Kac) in proteins of birch (<i>Betula platyphylla</i>) in response to drought using 4D label-free quantitative lys-acetylproteome analysis. We identified a total of 15 064 acetylated peptides across 4393 proteins, with 2486 Kac sites exhibiting significant changes: 246 prteins showed increased Kac levels, while 1406 displayed reductions. Notably, proteins associated with metabolic pathways, such as nucleotide sugar biosynthesis, proteasome, glutamate decarboxylase and the tricarboxylic acid (TCA) cycle, were significantly impacted. The alterations in Kac levels correlated with various KEGG pathways, suggesting that acetylation plays a regulatory role in drought response mechanisms. Furthermore, we identified specific acetylation sites in transcription factors (TFs), highlighting their involvement in this process. Functional validation demonstrated that mutations in Kac sites of five randomly selected TFs resulted in significant changes in drought tolerance, emphasizing the critical role of lysine acetylation in modulating stress responses. Overall, our findings indicate that Kac modification serves as a key regulatory mechanism in birch adaptation to drought stress, influencing both metabolic processes and transcriptional regulation.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into drought adaptation of the forage shrub Caragana korshinskii (Fabaceae) widely planted in drylands","authors":"Fengyuan Mei,&nbsp;Tianrui Yang,&nbsp;Haoyu Chao,&nbsp;Xiaohui Ma,&nbsp;Jingjing Wu,&nbsp;Qi Yang,&nbsp;Guangpeng Ren,&nbsp;Li Song,&nbsp;Qian Wang,&nbsp;Liwang Qi,&nbsp;Xinxing Fu,&nbsp; Gegentu,&nbsp;Cuiping Gao,&nbsp;Ruigang Wang,&nbsp;Ming Chen,&nbsp;Xiangwen Fang,&nbsp;Jianquan Liu,&nbsp;Guojing Li,&nbsp;Shengdan Wu","doi":"10.1111/tpj.17255","DOIUrl":"https://doi.org/10.1111/tpj.17255","url":null,"abstract":"<div>\u0000 \u0000 <p>The Korshinsk peashrub (<i>Caragana korshinskii</i>), known for its exceptional drought tolerance, is widely cultivated in arid and semi-arid regions for vegetation restoration and as a vital forage plant. To elucidate the genomic basis of its drought tolerance, we generated a chromosomal-scale genome sequence of <i>C. korshinskii</i>. Our synteny analysis disputes the previously hypothesized genus-specific whole-genome duplication event, as suggested by earlier transcriptome study of this species and its congeners. We identified that tandem duplications were critical for the expansion of gene families, such as early light-induced protein, heat shock protein 100, and Dehydrin, which are involved in cellular protection processes. These expansions are likely pivotal to the superior drought tolerance observed in <i>C. korshinskii</i>, as evidenced by the elevated gene expression of these genes under drought conditions. Furthermore, overexpression studies of seven tandemly duplicated <i>DHN</i> genes revealed a substantial enhancement in drought survival rates of seedlings, likely attributable to increased gene dosage effects. Conversely, gene silencing via virus-induced gene silencing demonstrated opposing effects. Additionally, we have established the CakorDB, a genomic resource database for <i>C. korshinskii</i> (https://bis.zju.edu.cn/cakordb/), accessible freely to the scientific community. Collectively, our study not only provides a valuable genomic resource for the Korshinsk peashrub but also highlights the genetic adaptations that enable <i>C. korshinskii</i> to thrive in desert environments, positioning its stress-responsive genes as a valuable genetic reservoir for breeding drought-resistant crops.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cis-prenyltransferase protein family in Taraxacum koksaghyz","authors":"Boje Müller,&nbsp;Eva Niephaus,&nbsp;Wolfgang Eisenreich,&nbsp;Jan Niklas Bröker,&nbsp;Richard M. Twyman,&nbsp;Dirk Prüfer,&nbsp;Christian Schulze Gronover","doi":"10.1111/tpj.17233","DOIUrl":"https://doi.org/10.1111/tpj.17233","url":null,"abstract":"<p>The <i>cis</i>-prenyltransferase (<i>cis</i>PT) enzyme family is involved in diverse biological processes that require the synthesis of linear isoprenoid compounds. <i>Taraxacum koksaghyz</i> is a rubber-producing species and potential crop that has eight <i>cis</i>PT homologs (TkCPT1–8) but their distribution and functions are unclear. We compared the structural organization and sequence homology of the proteins, and defined two groups: TkCPT and TkCPT-like (TkCPTL) proteins that form heteromeric <i>cis</i>PT enzymes (TkCPT1–4), and TkCPT proteins that function as homomeric <i>cis</i>PTs (TkCPT5–8). We found that <i>TkCPT1</i> and <i>TkCPT2</i> are predominantly expressed in latex whereas <i>TkCPT3</i> and <i>TkCPT6</i>-<i>8</i> are predominantly expressed in leaves. <i>TkCPT4</i> was constitutively expressed in all <i>T. koksaghyz</i> tissues and <i>TkCPT5</i> mRNA was detected in flowers. The TkCPT1–4 subunits localized to the endoplasmic reticulum whereas TkCPT5–7 were located in chloroplasts. TkCPT1-4 interacted with TkCPTL1-2, forming heteromeric complexes that complemented yeast lacking <i>cis</i>PT. Homomeric TkCPT6 could also complement yeast lacking <i>cis</i>PT but we observed no <i>cis</i>PT activity for TkCPT5, TkCPT7, or TkCPT8 in yeast functional complementation assays. TkCPT1/TkCPTL1 and TkCPT2/TkCPTL1 expressed in yeast produced extra-long-chain polyisoprenes, whereas TkCPT3/TkCPTL1 and TkCPT4/TkCPTL1 produced long-chain dolichols and polyisoprenes, TkCPT5 and TkCPT6 produced medium-chain polyisoprenes, and TkCPT7 and TkCPT8 catalyzed the formation of nerol. The complexity of <i>cis</i>PT proteins in <i>T. koksaghyz</i> suggests that they synthesize different metabolites in a tissue-specific manner, and thus play distinct roles in isoprenoid metabolism. This is the first comprehensive analysis of the localization, interactions, and products of the entire <i>T. koksaghyz cis</i>PT family <i>in vivo</i>, also revealing a novel pentaprenol found specifically in flowers.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring ABI5 regulation: Post-translational control and cofactor interactions in ABA signaling","authors":"Chang Du,&nbsp;Haiyan Bai,&nbsp;Yujie Yan,&nbsp;Yurui Liu,&nbsp;Xiangying Wang,&nbsp;Zhonghui Zhang","doi":"10.1111/tpj.17232","DOIUrl":"https://doi.org/10.1111/tpj.17232","url":null,"abstract":"<div>\u0000 \u0000 <p>Abscisic acid insensitive 5 (ABI5) is a pivotal transcription factor in abscisic acid (ABA) signaling, playing an essential role in plant growth and responses to abiotic stress. This key regulator is subject to multifaceted regulation, especially on post-translational mechanisms. Recent research has shed light on the post-translational regulation of ABI5, encompassing both post-translational modifications (PTMs) and the modulation of its transcriptional activity. In this review, we provide a comprehensive overview of the current knowledge surrounding the post-translational regulation of ABI5, along with the influence of various cofactors on its transcriptional activity and protein stability. The potential biological roles of PTMs of ABI5 in the context of ABA signaling and plant stress responses are also explored. As ABI5 is one of the most extensively studied proteins in the context of plant ABA signaling and environmental stress responses, a sophisticated and precise understanding of the regulatory mechanisms that govern ABI5 is not only beneficial for its application in genetic engineering but also helpful for our exploration in the fundamental principles of post-translational regulation.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of DICER-LIKE genes and their roles in Marchantia polymorpha development and salt stress response","authors":"Erika Csicsely,&nbsp;Anja Oberender,&nbsp;Anastasia-Styliani Georgiadou,&nbsp;Johanna Alz,&nbsp;Sebastian Kiel,&nbsp;Nora Gutsche,&nbsp;Sabine Zachgo,&nbsp;Jennifer Grünert,&nbsp;Andreas Klingl,&nbsp;Oguz Top,&nbsp;Wolfgang Frank","doi":"10.1111/tpj.17236","DOIUrl":"https://doi.org/10.1111/tpj.17236","url":null,"abstract":"<p>DICER-LIKE (DCL) proteins play a central role in plant small RNA (sRNA) biogenesis. The genome of the early land plant <i>Marchantia polymorpha</i> encodes four DCL proteins: MpDCL1a, MpDCL1b, MpDCL3, and MpDCL4. While MpDCL1a, MpDCL3 and MpDCL4 show high similarities to their orthologs in <i>Physcomitrium patens</i> and <i>Arabidopsis thaliana</i>, MpDCL1b shares only a limited homology with PpDCL1b, but it is very similar, in terms of functional domains, to orthologs in other moss and fern species. We generated Mp<i>dcl</i>^<i>ge</i> mutant lines for all Mp<i>DCL</i> genes with the CRISPR/Cas9 system and conducted phenotypic analyses under control, salt stress, and phytohormone treatments to uncover specific MpDCL functions. The mutants displayed severe developmental aberrations, altered responses to salt and phytohormones, and disturbed sexual organ development. By combining mRNA and sRNA analyses, we demonstrate that MpDCLs and their associated sRNAs play pivotal roles in regulating development, abiotic stress tolerance and phytohormone response in <i>M. polymorpha</i>. We identified MpDCL1a in microRNA biogenesis, MpDCL4 in trans-acting small interfering RNA generation, and MpDCL3 in the regulation of pathogen-related genes. Notably, salt sensitivity in <i>M. polymorpha</i> is dependent on MpDCL1b and Mp<i>dcl1b</i>^<i>ge</i> mutants display enhanced tolerance and reduced miRNA expression in response to salt stress. We propose that <i>M. polymorpha</i> employs specific mechanisms for regulating MpDCL1b associated miRNAs under high salinity conditions, potentially shared with other species harboring MpDCL1b homologs.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17236","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analysis reveals the allelic variation in JrWDRC2A9 and JrGPIAP conferring resistance against anthracnose (Colletotrichum gloeosporioides) in walnut (Juglans regia)","authors":"Andi Gong,&nbsp;Yuhui Dong,&nbsp;Shengyi Xu,&nbsp;Yutian Mu,&nbsp;Xichen Li,&nbsp;Chunyu Li,&nbsp;Qiang Liang,&nbsp;Jian Ning Liu,&nbsp;Changxi Wang,&nbsp;Ke Qiang Yang,&nbsp;Hongcheng Fang","doi":"10.1111/tpj.17254","DOIUrl":"https://doi.org/10.1111/tpj.17254","url":null,"abstract":"<div>\u0000 \u0000 <p>Walnut anthracnose induced by <i>Colletotrichum gloeosporioides</i> is a devastating disease that seriously threatens walnut cultivation. Screening novel resistance genes and exploring the molecular mechanisms are essential for disease-resistant genetic improvement of walnut. We conducted a genome-wide association studies of disease resistance traits based on the relative resistance index and single nucleotide polymorphisms (SNPs) obtained from 182 resequenced walnut accessions and 10 loci and corresponding candidate genes associated with resistance against <i>C. gloeosporioides</i> were identified. Then, through combined transcriptome analysis during <i>C. gloeosporioides</i> infection and qRT-PCR, we identified JrWDRC2A9 in SNP Chr13_36265784 loci and JrGPIAP in SNP Chr07_10106470 loci as two walnut anthracnose resistance genes. The validation of the disease resistance function of transgenic strains indicated that both JrWDRC2A9 and JrGPIAP promote walnut resistance to anthracnose. SNP Chr13_36265784 (A&gt;G) is located in the coding region of JrWDRC2A9 causing a glutamine (JrWDRC2A9^HapI) to arginine (JrWDRC2A9^HapII). Allelic variation in the WD domain attenuates JrWDRC2A9-mediated resistance against <i>C. gloeosporioides</i> and the binding affinity of JrWDRC2A9 for JrTLP1. On the contrary, the allelic variation caused by SNP Chr07_10106470 (T&gt;G) increased the walnut accessions resistance to <i>C. gloeosporioides</i> by promoting the expression level of JrGPIAP. Functional genomics revealed that JrGPIAP binds to the promoter of <i>JrPR1L</i> and activates its transcription, which is strengthened by the interaction between JrGPIAP and JrEMP24. These findings reveal the allelic variation in JrWDRC2A9 and JrGPIAP conferring resistance against <i>C. gloeosporioides</i>, providing a genetic basis for walnut disease resistance breeding in the future.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphate deficiency inducible OsGDPD5 affects root growth by regulating sugar-auxin crosstalk","authors":"Lokesh Verma,&nbsp;Mandavi Pandey,&nbsp;Chitra Bhatia,&nbsp;Poonam Mehra,&nbsp;Bhagat Singh,&nbsp;Jitender Giri","doi":"10.1111/tpj.17249","DOIUrl":"https://doi.org/10.1111/tpj.17249","url":null,"abstract":"<div>\u0000 \u0000 <p>Glycerophosphodiester phosphodiesterases (GDPDs) enzymes are known to be involved in phospholipids degradation pathways, where glycerophosphodiesters are hydrolyzed to glycerol-3-phosphate (G3P) and corresponding alcohol. In plants, GDPDs are involved in phosphate deficiency adaptive responses and have been shown to impact root length, but the precise mechanism remains unclear. This study focuses on the rice <i>GDPD5</i> gene and its role in regulating primary root growth. Our research demonstrates that <i>OsGDPD5</i> encodes a functional GDPD enzyme and could hydrolyze glycerophosphocholine and glycerophosphorylethanolamine. At transcriptional levels, <i>OsGDPD5</i> is preferentially expressed in the root tip and regulated by transcription factor OsPHR2. We have used CRISPR/Cas9 to generate <i>OsGDPD5</i> knock-out lines, allowing us to explore its role in root growth. Our findings show that <i>osgdpd5</i> mutants had a shorter primary root, which could be restored to a normal level by the exogenous application of sugar or G3P. Further, knocking out <i>OsGDPD5</i> alters endogenous levels of G3P and sugars, affecting auxin biosynthesis in the root and, ultimately, primary root growth. In this manner, OsGDPD5 has a crucial role in regulating physiological processes, specifically sugar and auxin signaling, which are known to be involved in root growth regulation in rice. Our research thus unraveled a link between rice phosphate deficiency-responsive lipid remodeling and root growth via sugar-hormone signaling.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"121 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}