The Plant Journal最新文献

{"title":"Expanding the BonnMu sequence-indexed repository of transposon induced maize (Zea mays L.) mutations in dent and flint germplasm.","authors":"Yan Naing Win, Tyll Stöcker, Xuelian Du, Alexa Brox, Marion Pitz, Alina Klaus, Hans-Peter Piepho, Heiko Schoof, Frank Hochholdinger, Caroline Marcon","doi":"10.1111/tpj.17088","DOIUrl":"https://doi.org/10.1111/tpj.17088","url":null,"abstract":"<p><p>The BonnMu resource is a transposon tagged mutant collection designed for functional genomics studies in maize. To expand this resource, we crossed an active Mutator (Mu) stock with dent (B73, Co125) and flint (DK105, EP1, and F7) germplasm, resulting in the generation of 8064 mutagenized BonnMu F₂-families. Sequencing of these Mu-tagged families revealed 425 924 presumptive heritable Mu insertions affecting 36 612 (83%) of the 44 303 high-confidence gene models of maize (B73v5). On average, we observed 12 Mu insertions per gene (425 924 total insertions/36 612 affected genes) and 53 insertions per BonnMu F₂-family (425 924 total insertions/8064 families). Mu insertions and photos of seedling phenotypes from segregating BonnMu F₂-families can be accessed through the Maize Genetics and Genomics Database (MaizeGDB). Downstream examination via the automated Mutant-seq Workflow Utility (MuWU) identified 94% of the presumptive germinal insertion sites in genic regions and only a small fraction of 6% inserting in non-coding intergenic sequences of the genome. Consistently, Mu insertions aligned with gene-dense chromosomal arms. In total, 42% of all BonnMu insertions were located in the 5' untranslated region of genes, corresponding to accessible chromatin. Furthermore, for 38% of the insertions (163 843 of 425 924 total insertions) Mu1, Mu8 and MuDR were confirmed to be the causal Mu elements. Our publicly accessible European BonnMu resource has archived insertions covering two major germplasm groups, thus facilitating both forward and reverse genetics studies.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The thylakoid phosphatase TEF8 is involved in state transition and high light stress resistance in Chlamydomonas.","authors":"Jie Dong, Jinrong Hou, Qiang Yao, Baoxiang Wang, Jingyi Wang, Xuan Shen, Ke Lai, Haitao Ge, Yingchun Wang, Min Xu, Aigen Fu, Fei Wang","doi":"10.1111/tpj.17108","DOIUrl":"https://doi.org/10.1111/tpj.17108","url":null,"abstract":"<p><p>The sophisticated regulation of state transition is required to maintain optimal photosynthetic performance under fluctuating light condition, through balancing the absorbed light energy between photosystem II and photosystem I. This exquisite process incorporates phosphorylation and dephosphorylation of light-harvesting complexes and PSII core subunits, accomplished by thylakoid membrane-localized kinases and phosphatases that have not been fully identified. In this study, one Chlamydomonas high light response gene, THYLAKOID ENRICHED FRACTION 8 (TEF8), was characterized. The Chlamydomonas tef8 mutant showed high light sensitivity and defective state transition. The enzymatic activity assays showed that TEF8 is a bona fide phosphatase localized in thylakoid membranes. Biochemical assays, including BN-PAGE, pull-down, and phosphopeptide mass spectrometry, proved that TEF8 associates with photosystem II and is involved in the dephosphorylation of D2 and CP29 subunits during state 2 to state 1 transition. Taken together, our results identified TEF8 as a thylakoid phosphatase with multiple dephosphorylation targets on photosystem II, and provide new insight into the regulatory mechanism of state transition and high light resistance in Chlamydomonas.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boron confers salt tolerance through facilitating BnaA2.HKT1-mediated root xylem Na+ unloading in rapeseed (Brassica napus L.)","authors":"Yingpeng Hua,&nbsp;Minnan Pei,&nbsp;Haili Song,&nbsp;Ying Liu,&nbsp;Ting Zhou,&nbsp;Hongbo Chao,&nbsp;Caipeng Yue,&nbsp;Jinyong Huang,&nbsp;Guangyong Qin,&nbsp;Yingna Feng","doi":"10.1111/tpj.17052","DOIUrl":"10.1111/tpj.17052","url":null,"abstract":"<div>\u0000 \u0000 <p>Boron (B) is an important limiting factor for plant growth and yield in saline soils, but the underlying molecular mechanisms remain poorly understood. In this study, we found that appropriate B supply obviously complemented rapeseed (<i>Brassica napus</i> L.) growth under salinity accompanied by higher biomass production and less reactive oxygen species accumulation. Determination of Na⁺ content in shoots and roots indicated that B significantly repressed root-to-shoot Na⁺ translocation, and non-invasive micro-tests of root xylem sap demonstrated that B increased xylem Na⁺ unloading in the roots of rapeseed plants under salinity. Comparative transcriptomic profiling revealed that B strongly upregulated <i>BnaHKT1s</i> expression, especially <i>BnaA2.HKT1</i>, in rapeseed roots exposed to salinity. <i>In situ</i> hybridizations analysis showed that <i>BnaA2.HKT1</i> was significantly induced in root stelar tissues by high B (HB) under salinity. Green fluorescent protein and yeast heterologous expression showed that BnaA2.HKT1 functioned as a plasma membrane-localized Na⁺ transporter. Knockout of <i>BnaA2.HKT1</i> by CRISPR/Cas9 resulted in hypersensitive of rapeseed plants to salinity even under HB condition, with higher shoot Na⁺ accumulation and lower biomass production. By contrast, overexpression of <i>BnaA2.HKT1</i> ameliorated salinity-induced growth inhibition under B deficiency and salinity. Overall, our results proposed that B functioned as a positive regulator for the rapeseed growth and seed production under salt stress through facilitating BnaA2.HKT1-mediated root xylem Na⁺ unloading. This study may also provide an alternative strategy for the improvement of crop growth and development in saline soils.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 4","pages":"1326-1342"},"PeriodicalIF":6.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Border-like cell formation mediated by SgPG1 confers aluminum resistance in Stylosanthes guianensis","authors":"Yan Lin,&nbsp;Guoxuan Liu,&nbsp;Pandao Liu,&nbsp;Qianqian Chen,&nbsp;Xueqiong Guo,&nbsp;Xing Lu,&nbsp;Zefei Cai,&nbsp;Lili Sun,&nbsp;Jiping Liu,&nbsp;Kang Chen,&nbsp;Guodao Liu,&nbsp;Jiang Tian,&nbsp;Cuiyue Liang","doi":"10.1111/tpj.17073","DOIUrl":"10.1111/tpj.17073","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Stylosanthes</i> is an important forage legume in tropical areas with strong resistance to aluminum (Al) toxicity, though knowledge of mechanisms underlying this resistance remains fragmentary. We found that border-like cells (BLCs) were constitutively produced surrounding the root tips of all 54 examined <i>Stylosanthes guianensis</i> genotypes, but not the <i>Stylosanthes viscose</i> genotype TF0140. In genotypic comparisons under Al conditions, the <i>S. guianensis</i> genotype RY#2 retained significantly more Al in BLCs and thereby showed higher relative root growth than TF0140. Formation of BLCs accompanied changes in cell wall pectin epitopes and differential expression of genes involved in pectin metabolism, including a <i>polygalacturonase</i> (<i>SgPG1</i>). The expression pattern of <i>SgPG1</i> was consistent with the formation of BLCs in both RY#2 and TF0140. SgPG1 was localized in cell walls and exhibited high activities mediating demethyl-esterified homogalacturonan degradation. Overexpressing <i>SgPG1</i> changed cell wall pectin epitopes, enhanced BLCs production, and Al resistance in both Arabidopsis and <i>Stylosanthes</i> hairy roots. Furthermore, combining protein-DNA binding assays <i>in vitro</i> and <i>in vivo</i>, a bHLH transcription factor SgbHLH19 was demonstrated to be the upstream regulator of <i>SgPG1</i>. Our study demonstrates that <i>S. guianensis</i> Al resistance mainly relies on BLCs, whose formation involves cell wall pectin epitope modification by SgPG1.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 4","pages":"1605-1624"},"PeriodicalIF":6.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DMFGAN: a multifeature data augmentation method for grape leaf disease identification","authors":"Yang Hu,&nbsp;Yukai Zhang,&nbsp;Shuai Liu,&nbsp;Guoxiong Zhou,&nbsp;Mingxuan Li,&nbsp;Yahui Hu,&nbsp;Johnny Li,&nbsp;Lixiang Sun","doi":"10.1111/tpj.17042","DOIUrl":"10.1111/tpj.17042","url":null,"abstract":"<div>\u0000 \u0000 <p>The use of deep learning techniques to identify grape leaf diseases relies on large, high-quality datasets. However, a large number of images occupy more computing resources and are prone to pattern collapse during training. In this paper, a depth-separable multifeature generative adversarial network (DMFGAN) was proposed to enhance grape leaf disease data. First, a multifeature extraction block (MFEB) based on the four-channel feature fusion strategy is designed to improve the quality of the generated image and avoid the problem of poor feature learning ability of the adversarial generation network caused by the single-channel feature extraction method. Second, a depth-based D-discriminator is designed to improve the discriminator capability and reduce the number of model parameters. Third, SeLU activation function was substituted for DCGAN activation function to overcome the problem that DCGAN activation function was not enough to fit grape leaf disease image data. Finally, an MFLoss function with a gradient penalty term is proposed to reduce the mode collapse during the training of generative adversarial networks. By comparing the visual indicators and evaluation indicators of the images generated by different models, and using the recognition network to verify the enhanced grape disease data, the results show that the method is effective in enhancing grape leaf disease data. Under the same experimental conditions, DMFGAN generates higher quality and more diverse images with fewer parameters than other generative adversarial networks. The mode breakdown times of generative adversarial networks in training process are reduced, which is more effective in practical application.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 4","pages":"1278-1303"},"PeriodicalIF":6.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Balancing act: The dynamic relationship between nutrient availability and plant defence.","authors":"Arka Dutta, Peter M Dracatos, Ghazanfar Abbas Khan","doi":"10.1111/tpj.17098","DOIUrl":"https://doi.org/10.1111/tpj.17098","url":null,"abstract":"<p><p>Plants depend heavily on soil nutrients for growth, development and defence. Nutrient availability is crucial not only for sustaining vital biochemical processes but also for mounting effective defences against a diverse array of pathogens. Macronutrients such as nitrogen, phosphorus and potassium significantly influence plant defence mechanisms by providing essential building blocks for the synthesis of defence compounds, immune signalling and physiological responses like stomatal regulation. Micronutrients like zinc, copper and iron are essential for balancing reactive oxygen species and other reactive compounds in plant immune responses. Although substantial circumstantial evidence links nutrient availability to plant defence, the molecular mechanisms underlying this process have only recently started to be understood. This review focuses on summarizing recent advances in understanding the molecular mechanisms by which nitrogen, phosphorus and iron interact with plant defence mechanisms and explores the potential for engineering nutritional immunity in crops to enhance their resilience against pathogens.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PbAGL7-PbNAC47-PbMYB73 complex coordinately regulates PbC3H1 and PbHCT17 to promote the lignin biosynthesis in stone cells of pear fruit.","authors":"Xin Gong, Kaijie Qi, Liangyi Zhao, Zhihua Xie, Jiahui Pan, Xin Yan, Katsuhiro Shiratake, Shaoling Zhang, Shutian Tao","doi":"10.1111/tpj.17090","DOIUrl":"https://doi.org/10.1111/tpj.17090","url":null,"abstract":"<p><p>Lignification of the cell wall in pear (Pyrus) fruit results in the formation of stone cells, which affects the texture and quality of the fruit. However, it is still unclear that how different transcription factors (TFs) work together to coordinate the synthesis and deposition of lignin. Here, we examined the transcriptome of pear varieties with different stone cell contents and found a key TF (PbAGL7) that can promote the increase of stone cell contents and secondary cell wall thicknesses. In addition, PbAGL7 can facilitate the expression level of lignin biosynthesis-related genes and accelerate the lignin biosynthesis in pear fruit and Arabidopsis. However, PbAGL7 did not directly bind to the promoters of PbC3H1 and PbHCT17 which are crucial genes involved in lignin biosynthesis. On the other hand, yeast two-hybrid (Y2H) library showed that PbNAC47 and PbMYB73 interacted with PbAGL7 in the nucleus. PbNAC47 and PbMYB73 also increased the stone cell and lignin contents, and upregulated the expressions of PbC3H1 and PbHCT17 by binding to the SNBE and AC elements, respectively. Moreover, PbNAC47 also interacted with PbMYB73 to form PbAGL7-PbNAC47-PbMYB73 complex. This complex significantly activated the expression levels of PbC3H1 and PbHCT17 and promoted lignin biosynthesis to form stone cells in pear fruit. Overall, our study provides new insights into the molecular mechanism of TFs that coordinately regulate the stone cell formation in pear fruit and extend our knowledge to understand cell wall lignification in plants.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daily glycome and transcriptome profiling reveals polysaccharide structures and correlated glycosyltransferases critical for cotton fiber growth.","authors":"Sivakumar Swaminathan, Corrinne E Grover, Alither S Mugisha, Lauren E Sichterman, Youngwoo Lee, Pengcheng Yang, Eileen L Mallery, Josef J Jareczek, Alexis G Leach, Jun Xie, Jonathan F Wendel, Daniel B Szymanski, Olga A Zabotina","doi":"10.1111/tpj.17084","DOIUrl":"https://doi.org/10.1111/tpj.17084","url":null,"abstract":"<p><p>Cotton fiber is the most valuable naturally available material for the textile industry and the fiber length and strength are key determinants of its quality. Dynamic changes in the pectin, xyloglucan, xylan, and cellulose polysaccharide epitope content during fiber growth contribute to complex remodeling of fiber cell wall (CW) and quality. Detailed knowledge about polysaccharide compositional and structural alteration in the fiber during fiber elongation and strengthening is important to understand the molecular dynamics of fiber development and improve its quality. Here, large-scale glycome profiling coupled with fiber phenotype and transcriptome profiling was conducted on fiber collected daily covering the most critical window of fiber development. The profiling studies with high temporal resolution allowed us to identify specific polysaccharide epitopes associated with distinct fiber phenotypes that might contribute to fiber quality. This study revealed the critical role of highly branched RG-I pectin epitopes such as β-1,4-linked-galactans, β-1,6-linked-galactans, and arabinogalactans, in addition to earlier reported homogalacturonans and xyloglucans in the formation of cotton fiber middle lamella and contributing to fiber plasticity and elongation. We also propose the essential role of heteroxylans (Xyl-MeGlcA and Xyl-3Ar), as a guiding factor for secondary CW cellulose microfibril arrangement, thus contributing to fiber strength. Correlation analysis of profiles of polysaccharide epitopes from glycome data and expression profiles of glycosyltransferase-encoding genes from transcriptome data identified several key putative glycosyltransferases that are potentially involved in synthesizing the critical polysaccharide epitopes. The findings of this study provide a foundation to identify molecular factors that dictate important fiber traits.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new angle for crop improvement? The RING E3 ubiquitin ligase LATA1 impacts tiller angle in rice","authors":"Martin Balcerowicz","doi":"10.1111/tpj.17069","DOIUrl":"10.1111/tpj.17069","url":null,"abstract":"&lt;p&gt;Rice (&lt;i&gt;Oryza sativa&lt;/i&gt;) is the staple crop for more than half the world's population, making yield improvements a critical goal for current breeding efforts. One approach focuses on improving plant architecture, and the tiller angle is a promising trait in this context: a large angle results in a spread-out growth habit that enhances light capture and helps outcompete weeds, while an erect growth habit with a smaller tiller angle allows for dense planting, efficient harvesting and reduces competition between individual plants (Wang et al., 2022). Domestication of wild rice has favoured an erect growth habit, which can be largely attributed to selection for specific alleles of several C2H2 zinc finger transcription factor genes, including &lt;i&gt;PROSTRATE GROWTH 1&lt;/i&gt; (&lt;i&gt;PROG1&lt;/i&gt;) (Tan et al., &lt;span&gt;2008&lt;/span&gt;), &lt;i&gt;PROG7&lt;/i&gt; (Hu et al., &lt;span&gt;2018&lt;/span&gt;) and &lt;i&gt;RICE PLANT ARCHITECTURE DOMESTICATION&lt;/i&gt; (&lt;i&gt;RPAD&lt;/i&gt;) (Wu et al., &lt;span&gt;2018&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;These genes also affect shoot gravitropism (Wang, Gao, Liang, Li, &amp; Wang, &lt;span&gt;2022&lt;/span&gt;), a process more thoroughly studied in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;. The starch-statolith hypothesis states that the direction of gravity is sensed by the sedimentation of starch-filled amyloplasts (Sack, &lt;span&gt;1997&lt;/span&gt;). This sedimentation, possibly sensed by mechanosensitive membranes, activates a signal transduction cascade that results in the asymmetric distribution of the plant hormone auxin across the shoot. High auxin concentrations on the lower side lead to increased cell elongation, causing asymmetric growth and a bending of the shoot (Takahashi et al., &lt;span&gt;2021&lt;/span&gt;). Roles for amyloplastic starch granules and asymmetric auxin distribution have also been established in the control of tiller angle in rice, but only a few genes involved in this process are known.&lt;/p&gt;&lt;p&gt;Lubin Tan's lab at China Agricultural University in Beijing studies the genetic regulation of rice agronomic traits; uncovering the signalling pathways underlying plant architecture thus remains a key area of the group's research. Through an EMS mutagenesis screen, PhD student Jinjian Fan, first author of the highlighted publication, and colleagues identified a mutant with a with a spread-out architecture they named &lt;i&gt;large tiller angle 1&lt;/i&gt; (&lt;i&gt;lata1&lt;/i&gt;). Throughout development, this mutant consistently exhibited larger tiller angles than the wild type, with the outermost tiller reaching a 25° angle at the heading stage, compared to an 11° angle in the wild type (Figure 1a). This phenotype was caused by reduced asymmetric growth at the tiller base. As for other tiller angle mutants, the change in angle was associated with altered shoot gravitropism: after rotating seedlings 90°, gravitropic bending was visibly delayed in &lt;i&gt;lata1&lt;/i&gt; seedlings (Figure 1b).&lt;/p&gt;&lt;p&gt;Using bulk segregant analysis, the authors mapped the &lt;i&gt;lata1&lt;/i&gt; phenotype to a single-nucleotide polymorphism (SNP) in a gene located on chromosome 5 tha","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 2","pages":"427-428"},"PeriodicalIF":6.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsAAH confers salt tolerance in rice seedlings.","authors":"Ting Xie, Jiangyu Xu, Wenling Hu, Silvtu Shan, Haoming Gao, Jiaxin Shen, Xinyi Chen, Yanxiao Jia, Xiuying Gao, Ji Huang, Hongsheng Zhang, Jinping Cheng","doi":"10.1111/tpj.17091","DOIUrl":"https://doi.org/10.1111/tpj.17091","url":null,"abstract":"<p><p>Soil salinization is becoming a great threat that reduces crop productivity worldwide. In this study, we found that rice allantoate amidohydrolase (OsAAH) expression was significantly upregulated by salt stress, and its overexpression conferred salt tolerance at the seedling stage. Compared to wild type (WT), the contents of ureides (allantoin and allantoate) were significantly increased in Osaah mutants and reduced in OsAAH overexpression lines both before and after salt treatments. Exogenous allantoin significantly promoted salt tolerance in OsAAH overexpression, but not in Osaah mutants. Subcellular localization showed that OsAAH was also localized to the peroxisomes in addition to the previously reported endoplasmic reticulum (ER). The differential expression of peroxisome-related genes was identified between Osaah mutants and WT. Furthermore, the contents of H₂O₂ and malondialdehyde (MDA) were significantly accumulated in Osaah mutants and reduced in OsAAH overexpression lines. The activities of antioxidant enzymes were significantly reduced in Osaah mutants and enhanced in OsAAH overexpression under NaCl treatment. The transcription factor OsABI5 could directly bind to OsAAH promoter and activate OsAAH expression. Our findings reveal that OsAAH could be induced by salt stress through the activation of OsABI5 and then confer salt tolerance by enhancing the scavenging capacity of reactive oxygen species (ROS), which contributes to rice breeding in salt tolerance.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":" ","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}