The Plant Journal最新文献

{"title":"The histone deacetylase BpHST1 influences branch angle in white birch (Betula platyphylla)","authors":"Lili Hou,&nbsp;Baoxin Li,&nbsp;Yueying Liu,&nbsp;Qingzhu Zhang,&nbsp;Zhimin Zheng","doi":"10.1111/tpj.71116","DOIUrl":"10.1111/tpj.71116","url":null,"abstract":"<p>Optimization of tree architecture is essential for advancing sustainable forestry, as appropriate canopy structure can contribute to growth and development. However, the molecular mechanisms that control tree architecture have not been fully characterized. While the putative NAD-dependent histone deacetylase HST1 was shown to regulate branching architecture in multiple crop species, whether epigenetic mechanism also influence tree architecture is unknown. Here, we investigated the genetic regulation of branching angle in white birch (<i>Betula platyphylla</i> Suk.), a pioneer tree of the temperate and boreal forests. Using <i>bphst1</i> mutants generated via gene editing, we demonstrate that BpHST1 is involved in tree architecture by modulating branching angles. We provide evidence that BpHST1 is a histone deacetylase associated with altered H3K27ac levels and that it affects the expression of the cellulase gene <i>CELLULASE 1</i> (<i>BpCEL1</i>). Compared with the wild type<i>, BpHST1</i> knockout and <i>BpCEL1</i> overexpression both resulted in reduced branch angles, supporting <i>BpCEL1</i> as a candidate downstream effector of BpHST1. Our study suggests that BpHST1 influences the birch architecture by altering H3K27 acetylation at the <i>BpCEL1</i> locus, elucidating a previously unknown aspect of the epigenetic mechanisms that control tree architecture in white birch.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13571764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148962690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A phosphatidic acid-based retrograde signaling pathway responsive to abiotic stress in Arabidopsis","authors":"Carrie Hiser,&nbsp;Ron Cook,&nbsp;Yosia Mugume,&nbsp;John E. Froehlich,&nbsp;Christoph Benning","doi":"10.1111/tpj.71107","DOIUrl":"10.1111/tpj.71107","url":null,"abstract":"<p>Due to their sessile nature, plants are constantly exposed to the environment and must cope with sometimes extreme changes in conditions during the day or growing season. Plants depend on photosynthesis as their primary means to generate energy and building blocks, and adverse environmental conditions can stress the photosynthetic apparatus leading to the production of toxic byproducts. In the long term, stress experienced by the chloroplast must be communicated to the nucleus to adjust the expression of genes providing robust abiotic stress resilience in a process called retrograde signaling. Here, we propose a retrograde signaling mechanism that starts with the accumulation of phosphatidic acid at the outer chloroplast membrane. A mutant of <i>Arabidopsis thaliana</i>, <i>lppγ lppɛ1</i>, disrupted in two chloroplast envelope membrane-located phosphatidic acid phosphatases, shows reduced growth and activation of abscisic acid-mediated abiotic stress-response pathways, among other changes, as determined by RNA-Seq analysis. The mutant is more resistant to freezing and osmotic stress. To identify components of the proposed retrograde signaling pathway, we conducted a suppressor screen in the <i>lppγ lppɛ1</i> mutant and identified a mutation that causes the loss of MED16, which is a component of Mediator, a transcriptional complex in the nucleus affecting the expression of genes involved in abiotic stress tolerance, among others. Based on these findings, we are proposing a lipid-based, retrograde signaling mechanism in response to abiotic stresses such as freezing.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13571837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148962737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The E2 ubiquitin-conjugating enzymes OsUBC11 and OsUBC12 promote rice seed germination by ubiquitinating and destabilizing OsABI3 in the ABA signaling pathway","authors":"Tian-Ci Xie,&nbsp;Tao Qing,&nbsp;Chuang Yang,&nbsp;Jia-Mu Wang,&nbsp;Hai-Ping Lu,&nbsp;Jian-Xiang Liu","doi":"10.1111/tpj.71096","DOIUrl":"10.1111/tpj.71096","url":null,"abstract":"<p>The ubiquitin-conjugating enzymes (E2) play critical roles in plant stress responses and development, but their functions in ABA-mediated seed germination in rice remain largely unknown. We identified two homologous E2 enzymes, OsUBC11 and OsUBC12, which are highly expressed in rice seeds and induced by multiple abiotic stresses including salt, osmotic stress, and ABA treatment. We demonstrated that OsUBC11 and OsUBC12 positively regulate rice seed germination, especially under NaCl and ABA treatment, as the double mutants exhibited delayed germination and hypersensitivity to ABA and salt, while overexpression lines showed accelerated germination and reduced sensitivity. OsUBC11 and OsUBC12 negatively regulate the expression of ABA-responsive genes, including <i>OsABI3</i>, <i>OsABI5</i>, <i>OsRAB21</i>, and <i>OsLEA3</i>. Protein interaction assays demonstrated that OsUBC11/12 directly interact with OsABI3, a key transcription factor in ABA signaling. OsUBC11/12 possess ubiquitin-conjugating activity and promote the ubiquitination and subsequent degradation of OsABI3, thereby reducing OsABI3 protein stability. Together, our findings establish that OsUBC11 and OsUBC12 positively regulate seed germination under salt stress by ubiquitinating and destabilizing OsABI3, thus attenuating ABA signaling.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MdATG5a-mediated autophagy improves apple performance under low light","authors":"Weijia Xiang,&nbsp;Yue Fang,&nbsp;Yanghao Long,&nbsp;Qiuxian Zhou,&nbsp;Ziyan Li,&nbsp;Xiaoqing Gong,&nbsp;Fengwang Ma","doi":"10.1111/tpj.71108","DOIUrl":"10.1111/tpj.71108","url":null,"abstract":"<p>Apples are sun-loving plants, and excessive canopy closure in orchards leads to poor ventilation and reduced light penetration, ultimately limiting yield and fruit quality. Autophagy is a conserved mechanism for recycling cellular materials and energy in eukaryotes and is known to participate in multiple stress responses. However, whether autophagy contributes to low-light adaptation remains largely unclear. Here, we show that <i>MdATG5a</i> enhances the tolerance of apple plants to low-light conditions. Under low light, <i>MdATG5a</i>-overexpressing (OE) plants exhibited higher photosynthetic capacity, greater biomass, and increased carbohydrate accumulation compared with wild-type (WT) plants. During acclimation to low light, OE plants enhanced light harvesting and promoted the turnover of photosynthetic proteins by increasing leaf area and the leaf nitrogen content. Relative to WT, OE plants displayed a lower light compensation point and light saturation point, reduced dark respiration rate, higher net photosynthetic rate and stomatal conductance, and less structural and functional damage to chloroplasts and photosystem II. Metabolite profiling further revealed that OE plants had more stable levels of flavonoids and carbohydrates and their derivatives. OE plants also showed higher autophagic activity than WT plants, and pharmacological inhibition of autophagy abolished the growth advantage of OE plants under low light. Moreover, we found that <i>MdATG5a</i> is positively regulated by MdPIF7, a key transcription factor in shade-related light signaling. Together, these findings indicate that <i>MdATG5a</i>-mediated autophagy, regulated by MdPIF7, is crucial for the adaptation of apple to low light by enhancing autophagic flux, improving photosynthetic performance, and stabilizing metabolic homeostasis.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis EMB2788/Npa1L1 is critical for pre-rRNA processing, ribosome biogenesis, and embryo development","authors":"Mei Liu,&nbsp;Shuailei Wang,&nbsp;Zhuoxuan Li,&nbsp;Zhaojiao Liu,&nbsp;Mengjuan Tong,&nbsp;Aiwei Zhang,&nbsp;Nan Chen,&nbsp;Yirui Zhu,&nbsp;Jingsheng Zhang,&nbsp;Lifen Chen,&nbsp;Yi Guo,&nbsp;Rui Li","doi":"10.1111/tpj.71114","DOIUrl":"10.1111/tpj.71114","url":null,"abstract":"<p>Ribosome assembly, a fundamental process for cellular functions, requires the precise processing of precursor rRNA (pre-rRNA) into mature rRNAs, a transformation guided by ribosome biogenesis factors (RBFs). While this process is well characterized in yeast and mammals, the roles of RBFs in pre-rRNA processing in plants remain poorly understood. Here, we report the characterization of Arabidopsis EMB2788, previously linked to embryonic development but functionally uncharacterized. We identified EMB2788 as the homolog of yeast nucleolar pre-ribosomal-associated protein 1 (Npa1p), a key pre-rRNA processing regulator, and named it Npa1L1. <i>npa1l1</i> embryos exhibited severe defects from the late globular stage onward, including altered cell division planes, slowed division rates, and a consequent disruption of bilateral symmetry, accompanied by delayed endosperm development, although a small proportion of embryos developed into morphologically abnormal seedlings. Npa1L1 was likely a nucleolar protein and its loss of function led to an increased abundance of 40S subunits and a reduced abundance of 60S subunits and 80S monosomes. Molecular analysis showed pronounced accumulation of 35S pre-rRNA and 18S rRNA precursors (P-A₃) and a reduction in mature 18S rRNA levels. Furthermore, co-immunoprecipitation experiments indicated that Npa1L1 physically interacted with ribosome biogenesis factors, including the DEAD-box RNA helicases RH7 and RH27, and the GTPase NSN1. Collectively, our findings uncover that Npa1L1 is a crucial ribosome biogenesis factor in Arabidopsis that contributes to pre-rRNA processing, ribosomal subunit homeostasis, and embryo development. Nevertheless, the precise function of Npa1L1 in rRNA processing, and whether it differs from that of yeast Npa1p, requires further investigation.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Master manipulator: small brown planthopper tailors expression of salivary protein to evade plant immunity","authors":"Brian C. Mooney","doi":"10.1111/tpj.71098","DOIUrl":"10.1111/tpj.71098","url":null,"abstract":"&lt;p&gt;The small brown planthopper (SBPH) &lt;i&gt;Laodelphax striatellus&lt;/i&gt; is a herbivorous insect and highly destructive pest of multiple crops (Li et al., &lt;span&gt;2023&lt;/span&gt;). SBPH feeds on the contents of the phloem as a ‘sap-sucker’, impeding plant growth and facilitating the spread of viruses. To protect themselves against insect herbivory, plants rely on physical barriers and a range of inducible cellular defence responses. These are triggered by the recognition of insect-derived components, including proteins secreted in saliva during feeding. For example, perception of the salivary protein Mp10 from the green peach aphid leads to the production of reactive oxygen species (ROS) and the accumulation of defence hormones (Rodriguez et al., &lt;span&gt;2014&lt;/span&gt;). In contrast, salivary proteins may also function as crucial ‘effectors’ that promote herbivory by suppressing defence responses of non-adapted plant hosts. This results in a trade-off where the immune-suppressing benefit of an effector must be weighed against the risk of triggering additional defence responses.&lt;/p&gt;&lt;p&gt;Although SBPH can feed on a wide range of plants, rice is its preferred host, providing optimal conditions for maximal survival and fecundity. Maize serves as a suboptimal host that supports feeding but cannot sustain SBPH's full life cycle (Li et al., &lt;span&gt;2009&lt;/span&gt;). Researchers Yongjun Zhang at Nanjing Agricultural University and Zewen Liu at the Institute of Plant Protection in Beijing collaborated in a recent study to investigate the strategies used by SBPH to infest these different plant hosts (Teng et al., &lt;span&gt;2026&lt;/span&gt;). In an initial screen, the authors examined host-dependent differences in the expression of putative SBPH salivary effectors. While the majority of SBPH genes encoding known salivary proteins did not exhibit host-dependent differences, the transcript &lt;i&gt;LsG40&lt;/i&gt; was markedly more abundant in SBPH feeding on the optimal host rice compared with the suboptimal host maize (Figure 1a). This gene is predominantly expressed in the salivary glands and highly enriched in the feeding stages of development compared to the non-feeding egg stage. Together with the presence of a signal peptide for secretion, this data indicates that &lt;i&gt;LsG40&lt;/i&gt; encodes a salivary protein with a host-dependent expression pattern.&lt;/p&gt;&lt;p&gt;To investigate the role of LsG40 &lt;i&gt;in planta&lt;/i&gt;, an LsG40-GFP fusion protein was transiently expressed in &lt;i&gt;Nicotiana benthamiana&lt;/i&gt;. LsG40-GFP triggered plant defence responses including cell death (Figure 1b) and ROS accumulation, suggesting that this protein is recognised by plant cells as an immune elicitor. These induced defences bolstered plant resistance against the oomycete pathogen &lt;i&gt;Phytophthora capsici&lt;/i&gt; and rendered plants expressing LsG40 less attractive to herbivory by the fall armyworm (&lt;i&gt;Spodoptera frugiperda&lt;/i&gt;) compared to a GFP-expressing control.&lt;/p&gt;&lt;p&gt;The authors next evaluated the effects of LsG40 on SBPH's natural host","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13557015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A plant-centric view of class B flavin-dependent monooxygenase evolution and diversity","authors":"Joachim Møller Christensen,&nbsp;Elizabeth H. J. Neilson","doi":"10.1111/tpj.71106","DOIUrl":"10.1111/tpj.71106","url":null,"abstract":"<p>Flavin-dependent monooxygenases (FMOs) are ancient enzymes present throughout all kingdoms of life. FMOs utilize flavin-based cofactors to incorporate an oxygen atom into their substrate, altering its chemical properties. Class B FMOs are enriched throughout the plant kingdom, catalyzing essential reactions for plant development and defense, including auxin biosynthesis and systemic acquired resistance. Despite these essential metabolic roles, class B FMO functional characterization remains relatively limited, with no common evolutionary framework for FMO diversity across the plant kingdom currently available. By mining genomes representing 78 major Viridiplantae lineages, we present a curated dataset and comprehensive plant-centric phylogenetic analysis of class B FMOs, classifying eight distinct families: BVMO, N-Ox, N-Ox like 1, SeedlessFMO, S-Ox, S-Ox like 1, S-Ox like 2, and YUCCA. Most families evidently originate from an algal progenitor, although disparate degrees of FMO prevalence within and across families suggest multiple acquisition events in plants via horizontal gene transfer. Structural modeling and domain architecture analysis provide a refined framework for class B FMO diversity in plants delivering new insights into protein diversity and evolution. This plant-centric focus on class B FMOs provides an important resource that will facilitate further biochemical and functional characterization within plant development and response to environmental change.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148917746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CPKs-CBP60b positive feedback loop regulates immunity in Arabidopsis","authors":"Lu-Shen Li,&nbsp;Lin Ma,&nbsp;Yun-Xia Chen,&nbsp;Sha Li","doi":"10.1111/tpj.71100","DOIUrl":"https://doi.org/10.1111/tpj.71100","url":null,"abstract":"<p>Calmodulin-binding protein 60b (CBP60b) is a pivotal transcription factor in the transcriptional reprogramming of immune responses. Although its downstream regulatory genes and associated genetic pathways have been reported, its post-translational regulation and modifications remain unexplored. Here, we report that calcium-dependent protein kinases CPK4/5/6/11 physically interact with CBP60b and phosphorylate its serine 568 residue, thereby enhancing its transcriptional activity. The phosphodeficient variant CBP60b^S568A compromises immune responses, whereas the phosphomimetic variant CBP60b^S568D enhances resistance against pathogens, demonstrating that ser568 is essential for the function of CBP60b. Moreover, we observed that the expression of <i>CPK4/5/6/11</i> is induced by pathogens, while this pathogen-induced expression of <i>CPKs</i> is reduced in the <i>cbp60b</i> mutant. Through experiments including dual luciferase (LUC) reporter assays and chromatin immunoprecipitation (ChIP) assays, we further demonstrated that <i>CPK4</i>, <i>CPK5</i>, and <i>CPK11</i> are direct transcriptional targets of CBP60b. In conclusion, our results demonstrate for the first time that CBP60b is post-translationally regulated by CPKs via phosphorylation, while the pathogen-induced expression of <i>CPKs</i> is dependent on CBP60b. This mutual regulation establishes a positive feedback loop, which enables plants to mount a robust immune response against pathogens.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148904063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis STN7/STN8 alleviates oxidative damages via different regulatory patterns under drought stress","authors":"Hao-Tian Mao,&nbsp;Ying Qin,&nbsp;Lun-Xing Chen,&nbsp;Yi-Cheng Zhang,&nbsp;Fu-Yun Wang,&nbsp;Shu Yuan,&nbsp;Shozeb Haider,&nbsp;Yang-Er Chen","doi":"10.1111/tpj.71101","DOIUrl":"https://doi.org/10.1111/tpj.71101","url":null,"abstract":"<p>STN7 and STN8 kinases phosphorylate several proteins of the photosynthetic apparatus in response to changes in environmental conditions. Phosphorylation is linked to the dynamic arrangement of both the 3D structure of the thylakoid membrane and thylakoid protein complexes, thereby controlling excitation energy transfer between photosystems and the repair cycle of photosystem II (PSII). However, mutants with phosphorylation of PSII core proteins show only minor changes compared with the wild-type (WT) under conditions tested so far, and thus the physiological significance of reversible thylakoid protein phosphorylation by STN8 remains largely unresolved. In this study, we examined the impact of STN7 and STN8 on osmotic stress and consequent changes in the structure of the thylakoid membrane in <i>Arabidopsis thaliana</i> using physiological, cellular, and biochemical assays. Our results demonstrate that Arabidopsis plants lacking STN7 or STN8 exhibited slow growth and impaired regulation of excitation energy transfer, as well as low photosynthetic efficiency, excessive accumulation of ROS, and more severe cell death in response to osmotic stress compared with the WT. Clear effects of osmotic stress were observed in both <i>stn7</i> and <i>stn8</i> mutants but were more pronounced in the <i>stn7stn8</i> double mutant. Our data strongly suggest that phosphorylation of the PSII reaction center proteins, regulated by STN8, is important for maintaining the functional balance of the photosynthetic apparatus under osmotic stress-induced changes in the thylakoid membrane and thus protects it from oxidative stress.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148904064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SlBBX19 modulates plant development and negatively regulates chilling tolerance in tomato","authors":"Yunfei Liang,&nbsp;Fanyi Meng,&nbsp;Hongwei Sun,&nbsp;Hongyan Shi,&nbsp;Fang Ma,&nbsp;Xiangqiang Zhan","doi":"10.1111/tpj.71099","DOIUrl":"10.1111/tpj.71099","url":null,"abstract":"<p>Chilling stress severely restricts tomato cultivation and productivity, yet the mechanisms by which a single regulator integrates photosynthetic efficiency, developmental progression, and chilling tolerance remain to be elucidated. Here, we characterized <i>SlBBX19</i>, a chilling-inducible B-box transcription factor in tomato (<i>Solanum lycopersicum</i>). SlBBX19 is nucleus-localized, functions as a negative regulator of vegetative growth and photosynthetic capacity under normal conditions, while simultaneously promoting fruit ripening and suppressing inflorescence branching. Under chilling stress, <i>SlBBX19</i> acts as a negative regulator of chilling tolerance: overexpression exacerbates reactive oxygen species (ROS) accumulation, membrane damage, and suppresses <i>COR</i> pathway activation, whereas knockout lines exhibit enhanced chilling resistance. Transcriptomic and molecular analyses reveal that SlBBX19 directly binds to G-box elements in the promoters of light-harvesting chlorophyll a/b-binding genes <i>SlLhcb2.2</i> and <i>SlLhca4.1</i> to repress their transcription. Virus-induced gene silencing of these targets recapitulated the overexpression phenotypes, confirming that <i>SlBBX19</i> compromises photosynthetic efficiency and chilling tolerance primarily through <i>SlLhc</i> downregulation. Collectively, our findings establish <i>SlBBX19</i> as a key integrator of development and environmental acclimation, revealing a novel regulatory module that links photosynthetic antenna dynamics to chilling stress adaptation. This work may provide a promising genetic target for engineering chilling-tolerant and physiologically optimized tomato varieties.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"127 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148899959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}