RNA加工/修饰酶在拟南芥对热精胺的反应中起关键作用

IF 5.7 1区 生物学 Q1 PLANT SCIENCES
Mitsuru Saraumi, Takahiro Tanaka, Daiki Koyama, Yoshitaka Nishi, Yoshihiro Takahashi, Hiroyasu Motose, Taku Takahashi
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引用次数: 0

摘要

热精胺通过增强拟南芥SAC51基因家族mrna的翻译参与木质部分化的负向调控。这些mrna包含保守的上游开放阅读帧(uorf),干扰主ORF的翻译。为了研究热精胺在这一过程中的作用机制,我们分离了对热精胺不敏感的突变体,命名为its。我们发现导致这些突变的四个基因its1到its4编码:(i) SPOUT RNA甲基转移酶的同源物,(ii) rRNA伪尿苷合成酶CBF5/NAP57, (iii)假设的剪接体拆卸因子STIPL1/NTR1,以及(iv)植物特异性RNA结合蛋白PHIP1。这四种突变体的热精胺含量比野生型高得多。虽然除了its1外,所有这些突变体看起来几乎正常,但它们增强了编码热精胺合酶的ACL5突变体的矮化表型,导致类似于SAC51家族成员ACL5和SACL3的双重敲除的微小植物。报告分析显示,CaMV 35S启动子- sac51 ' -GUS融合结构的GUS活性在its s1和its4中显著降低或在its s2和its3中不受影响,而在its s1、its3和its4中略有增加,或在its s2中没有变化。这些发现强调了RNA加工和修饰在含uorf转录物的热精胺依赖的翻译调节中的关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

RNA processing/modifying enzymes play key roles in the response to thermospermine in Arabidopsis thaliana

RNA processing/modifying enzymes play key roles in the response to thermospermine in Arabidopsis thaliana

Thermospermine is involved in negative regulation of xylem differentiation by enhancing the translation of mRNAs of the SAC51 gene family in Arabidopsis (Arabidopsis thaliana). These mRNAs contain conserved upstream open reading frames (uORFs) that interfere with the translation of the main ORF. To investigate the mechanism by which thermospermine acts in this process, we isolated mutants insensitive to thermospermine, named ‘its’. We show that the four genes responsible for these mutants, its1 to its4, encode: (i) a homolog of SPOUT RNA methyltransferase, (ii) an rRNA pseudouridine synthase CBF5/NAP57, (iii) a putative spliceosome disassembly factor STIPL1/NTR1, and (iv) a plant-specific RNA-binding protein PHIP1. These four mutants were found to have much higher levels of thermospermine than the wild-type. While all these mutants except its1 appear almost normal, they enhance the dwarf phenotype of a mutant of ACL5, which encodes thermospermine synthase, resulting in tiny plants resembling a double knockout of ACL5 and SACL3, a member of the SAC51 family. Reporter assays revealed that GUS activity from the CaMV 35S promoter-SAC51 5′-GUS fusion construct was significantly reduced in its1 and its4 or not affected in its2 and its3, while it was slightly increased in its1, its3, and its4, or not changed in its2 by thermospermine. These findings underscore the critical role of RNA processing and modification in the thermospermine-dependent translational regulation of uORF-containing transcripts.

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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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