The Plant Journal最新文献

{"title":"A well-annotated genome of Apium graveolens var. dulce cv. Challenger, a celery with resistance to Fusarium oxysporum f. sp. apii race 2","authors":"Chaehee Lee,&nbsp;Lynn Epstein,&nbsp;Sukhwinder Kaur,&nbsp;Peter M. Henry,&nbsp;A. Dorien Postma-Haarsma,&nbsp;J. Grey Monroe,&nbsp;Allen Van Deynze","doi":"10.1111/tpj.70251","DOIUrl":"https://doi.org/10.1111/tpj.70251","url":null,"abstract":"<p>Celery (A<i>pium graveolens</i> var. <i>dulce)</i> production can be limited by the fungal pathogen <i>Fusarium oxysporum</i> f. sp. <i>apii</i> (<i>Foa</i>), particularly at temperatures above 22°C. Because celery has a narrow genetic base, an intraspecific admixture of <i>Apium graveolens</i> was developed into cv. Challenger, which is resistant to <i>Foa</i> race 2, the causal agent of <i>Fusarium</i> yellows, but susceptible to <i>Foa</i> race 4, a relatively unrelated causal agent of <i>Fusarium</i> wilt. We assembled a high-quality, chromosome-level physical map of Challenger with 40 464 RNA-based, protein-coding gene models in 3.3 Gbp and anchored it with a genetic map. Although there is high gene density and higher recombination at the ends of the chromosomes, an average of 56% of the genes/chromosome are in lower recombination zones (&lt;0.025 cM/Mb). We identified Challenger's nucleotide-binding and leucine-rich repeat receptors (NLRs) and pattern recognition receptors (PRRs), the two gene families that encode most resistance (R) genes. In three treatment groups (mock-infested or infested with either <i>Foa</i> race 2 or race 4), 243 NLRs and 445 PRRs were quantified in the celery crowns via Quant-Seq 3′ mRNA-Seq (Tag-Seq). We compared the genomes of Challenger with that of the previously published cv. Ventura, which is moderately susceptible to <i>Foa</i> race 2. We present a toolbox for genome-assisted breeding for celery that includes annotated gene models, a protocol for genotype-by-sequencing, documentation of the expression of NLRs and PRRs, and a straightforward strategy for introgressing selected NLR superclusters, 83% of which are in higher recombination regions.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decreasing protein biotinylation background in a diatom facilitates proximity labeling of the periplastidial compartment proteome","authors":"Jiahuan Zhang,&nbsp;Tianjun Cao,&nbsp;Yanyou Jiang,&nbsp;Yue Feng,&nbsp;Kangning Guo,&nbsp;Jin Yang,&nbsp;Huan Zhang,&nbsp;Xiaobo Li","doi":"10.1111/tpj.70259","DOIUrl":"https://doi.org/10.1111/tpj.70259","url":null,"abstract":"<div>\u0000 \u0000 <p>Diatoms are ecologically and industrially significant microalgae, crucial for global carbon fixation and biotechnological applications. Their complex plastid membrane structures, resulting from secondary endosymbiosis, remain poorly characterized, particularly the periplastidial compartment (PPC). Proximity labeling techniques, such as TurboID and ascorbate peroxidase 2 (APEX2)-based labeling, are powerful tools for identifying protein–protein interactions and spatial proteomes, but their application in diatoms is hindered by unknown factors. In this study, we identified and characterized the high biotinylation background in diatoms, including <i>Phaeodactylum tricornutum</i> and other microalgae, which significantly impairs the effectiveness of proximity labeling. We also characterized the biotin synthase (BIOB) in <i>P. tricornutum</i>, a key enzyme in biotin biosynthesis. By using a <i>biob</i> mutant to deplete biotin, we successfully decreased the biotinylation background, enhancing the sensitivity and quality of proximity labeling. Applying this approach to the PPC, we identified several proteins previously undetectable through bioinformatics and confocal microscopy. Our results demonstrate that inhibiting biotin synthesis improves TurboID-based proximity labeling methods for studying protein interactions and spatial proteomics in diatoms. The case study of the improved proximity labeling system in PPC also increased our understanding of the complex plastids derived from higher-order endosymbiosis.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition","authors":"Pilar Prieto-Dapena,&nbsp;Raúl Carranco,&nbsp;Concepción Almoguera,&nbsp;Juan Jordano","doi":"10.1111/tpj.70261","DOIUrl":"https://doi.org/10.1111/tpj.70261","url":null,"abstract":"<p>HSFA9 (A9) is a seed-specific transcription factor that contributes to seed longevity in sunflower. A9 also links the regulation of seed maturation with that of early seedling greening through its effects on various light receptors. Transcriptomic analyses of transgenic tobacco seeds suggested that A9 might affect chromatin remodeling. Here, using formaldehyde-assisted isolation of regulatory elements (FAIRE) and micrococcal nuclease digestion, we analyzed the A9 effects on chromatin accessibility shortly after seed imbibition in the proximal promoter region of developmentally relevant genes, including HY5 and PHYA. A9, expressed from a seed-specific promoter, enhanced chromatin accessibility in the analyzed regions. Converse, promoter-specific, effects were observed upon loss-of-function of tobacco A9 (NtA9) in transgenic seeds. Furthermore, a memory effect was observed, as the induced chromatin accessibility persisted for up to 4 days after seed imbibition, when A9 was no longer detected. The A9-induced chromatin effects involved labile/unstable nucleosomes placed at proximal promoter locations where A9 induced substantial nucleosomal depletion. Specific inhibitors of BRAHMA-like ATPase subunits of SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin-remodeling complexes and histone deacetylase (HDAC) impaired A9-induced memory. Thus, SWI/SNF remodeling and HDAC activity mechanistically contribute to the A9-induced memory. Furthermore, SWI/SNF inhibition specifically reduced the HY5 and PHYA promoter accessibility in both transgenic and non-transgenic seeds. Our results identify HSFA9 as a potential master, short-term, ‘epigenetic’ regulator that operates in seeds in anticipation of seedling establishment. The new, A9-induced, somatic memory effect reported here may facilitate early seedling greening and stress tolerance during the seed-to-seedling developmental transition.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70261","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability and function of rice OsHRZ ubiquitin ligases are regulated at multiple sites by the iron–zinc balance","authors":"Haruka Shinkawa,&nbsp;Takanori Kobayashi,&nbsp;Akari Murota,&nbsp;Ayane Kamijima,&nbsp;Naoko K. Nishizawa","doi":"10.1111/tpj.70258","DOIUrl":"https://doi.org/10.1111/tpj.70258","url":null,"abstract":"<div>\u0000 \u0000 <p>Iron (Fe) is essential for all living organisms, including plants. Plants upregulate the expression of several genes involved in Fe uptake and translocation in response to Fe deficiency. Hemerythrin motif-containing RING and zinc (Zn)-finger proteins (HRZs) are potential intracellular Fe sensors in plants. Rice (<i>Oryza sativa</i>) OsHRZs are ubiquitin ligases containing hemerythrin domains, a CHY Zn-finger, a CTCHY Zn-finger, a RING Zn-finger, and a rubredoxin-type fold, with each capable of binding Fe or Zn. In this study, we explored whether the OsHRZs sense Fe status by modulating their function depending on metal binding to these domains. The OsHRZ1 protein overexpressed in rice was rapidly degraded in roots, primarily because of the instability of the C-terminal domains. An N-terminal OsHRZ1 sequence with two hemerythrin domains was stably overexpressed in rice and exhibited an enhanced Fe-deficiency response in a dominant-negative manner. CRISPR/Cas9-based rice lines harboring mutations at the C-terminal RING Zn-finger domain or rubredoxin-type fold of <i>OsHRZ1</i> or <i>OsHRZ2</i> exhibited Fe-deficiency tolerance and Fe accumulation in grains, indicating the important function of these domains <i>in planta</i>. OsHRZ1 and OsHRZ2 proteins were degraded <i>in vitro</i> through the proteasome pathway, which was dependent on the OsHRZ1 RING Zn-finger domain. Degradation was enhanced under Fe-deficient conditions or with excess Zn bound to the OsHRZ1 protein. The self-ubiquitinating activity of OsHRZ1 and OsHRZ2 was enhanced by excess Zn binding. The results suggest that the stability and function of OsHRZs are affected by Fe nutritional conditions through Zn or Fe binding to multiple domains in the OsHRZ proteins.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering an RNA/protein-binding module for higher transgene protein production and improved long-term durability","authors":"Yu-Hung Hung,&nbsp;Eric Wang,&nbsp;Tayahna Agtarap,&nbsp;Gerald Klaas,&nbsp;R. Keith Slotkin","doi":"10.1111/tpj.70254","DOIUrl":"https://doi.org/10.1111/tpj.70254","url":null,"abstract":"<div>\u0000 \u0000 <p>Improvement and research of plants depends on the long-term expression of transgenes. However, the durability of transgene expression is routinely hampered by silencing pathways that start as the post-transcriptional process of mRNA degradation by RNA interference (RNAi). To avoid transgene silencing, we aimed to inhibit the sorting of transgene mRNAs into RNAi. We manipulated a well-studied protein/RNA-binding module from Arabidopsis into a transgene transcript, where the transcript is now bound by an engineered RNA-binding protein that preferentially sorts the RNA into translation. We used the Cas9 transcript as a proof-of-principle and demonstrated higher Cas9 protein production and gene editing rates. In addition, transgenes with the engineered protein/RNA-binding module had improved long-term durability of transgene expression, as after several inbred generations these plants had higher Cas9 protein accumulation and lower levels of DNA methylation, a hallmark of transgene silencing. Our engineered system represents a successful manipulation of post-transcriptional RNA sorting for improved transgene performance, and could be applied to any transgene transcript.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel small cysteine-rich protein, PbEL04, identified from Plasmodiophora brassicae, associates with AtSAMS2 to regulate Arabidopsis clubroot resistance","authors":"Xuefei Jiang,&nbsp;Peiyao Li,&nbsp;Xinmeng Li,&nbsp;Xinrui Xu,&nbsp;Ying Su,&nbsp;Yu Fu,&nbsp;Zihan Ni,&nbsp;Shuang Lu,&nbsp;Shifan Wu,&nbsp;Mingting Wu,&nbsp;Ziyue Xu,&nbsp;Tianyu Wang,&nbsp;Xinyi Dang,&nbsp;Maolin Wang,&nbsp;Rui Wang","doi":"10.1111/tpj.70264","DOIUrl":"https://doi.org/10.1111/tpj.70264","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Plasmodiophora brassicae</i> is a devastating intracellular pathogen that causes clubroot disease in <i>Brassicaceae</i> plants, leading to significant economic losses in agriculture. Enhancing plant resistance against <i>P. brassicae</i> has become a crucial global challenge. In this study, we report a new secreted protein, PbEL04, which can trigger cell death associated with H₂O₂ accumulation and electrolyte leakage in the non-host plant <i>Nicotiana benthamiana</i> but enhances the resistance to <i>P. brassicae</i> when overexpressed in <i>Arabidopsis thaliana.</i> Subsequently, AtSAMS2, which interacts with PbEL04, was identified in a screen of a Y2H library and confirmed by Co-IP as well as BiFC, and the expression of <i>AtSAMS2</i> was significantly increased during the cortical infection stage. Furthermore, overexpressing <i>AtSAMS2</i> in <i>Arabidopsis</i> exhibited higher susceptibility to <i>P. brassicae</i> than the wild type, while the mutant showed significantly elevated resistance without affecting plant growth and development, suggesting that AtSAMS2 could be a negative factor in resistance to <i>P. brassicae</i> infection. Taken together, our results suggest that <i>P. brassicae</i> infection secretes an effector protein, PbEL04, which could interact with AtSAMS2 and affect the hormone signaling pathways to influence the formation of root swelling. Here, we discovered a new effector protein, PbEL04, and identified its host interactor, AtSAMS2, which holds great potential in plant breeding as a key genetic target for clubroot resistance.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcription factor ZmNLP8 modulates nitrate utilization by transactivating ZmNiR1.2 in maize","authors":"Longjiang Gu,&nbsp;Qiuyu Cao,&nbsp;Jinlei Dong,&nbsp;Mengyun Qiao,&nbsp;Zimeng Wang,&nbsp;Zhen Zhang,&nbsp;Hongchen Sun,&nbsp;Haoxun Xie,&nbsp;Min Ge,&nbsp;Yeqing Zhang,&nbsp;Huan Xu,&nbsp;Weina Si,&nbsp;Beijiu Cheng,&nbsp;Xiaoyu Li,&nbsp;Haiyang Jiang","doi":"10.1111/tpj.70263","DOIUrl":"https://doi.org/10.1111/tpj.70263","url":null,"abstract":"<div>\u0000 \u0000 <p>The excessive use of nitrogen fertilizer boosts agricultural production, but poses serious threats to the environment. It therefore highlights an urgent need to achieve the required yield increases while reducing nitrogen fertilizer use. Improving crop nitrogen use efficiency (NUE) is regarded as a promising approach to solve this dilemma. Here, we show that loss-of-function mutations in maize NIN-like protein 8 (<i>ZmNLP8</i>) cause earlier senescence and result in over 70% grain yield losses per plant under normal nitrogen nutrition in field trials, while the transgenic line that overexpressed <i>ZmNLP8</i> exhibits remarkable growth vigor in fluctuating nitrate conditions, indicating that ZmNLP8 is indispensable in regulating nitrogen utilization in maize. ZmNLP8, acting as a transcription factor, binds to the nitrate-responsive <i>cis</i>-element and transactivates the expression of <i>ZmNiR1.2</i>. Moreover, a pull-down assay, combined with luciferase complementation assay and mass spectrometry analysis, revealed that ZmNLP8 physically interacted with ZmTCP8. Knockout of <i>ZmTCP8</i> impairs vegetative growth under different nitrate conditions, indicating that ZmTCP8 functions in a common pathway as ZmNLP8. The dual-luciferase transient expression system reveals that ZmNLP8 synergistically associates with ZmTCP8 to regulate the expression of <i>ZmNiR1.2</i>. Analysis of a panel of 1210 maize germplasms reveals that 92.6% of modern improved maize lines carry abundant variations and have larger nucleotide difference per site (Dxy) in the <i>ZmNLP8</i> promoter, which results in a reduction in the transcriptional activity of <i>ZmNLP8</i>. Our results demonstrate that ZmNLP8 has probably been selected through breeding and plays a pivotal role in nitrogen utilization, at least in part, by regulating the expression of <i>ZmNiR1.2</i>.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SlBTA2 is required for cuticle biosynthesis in tomato fruit","authors":"Yao Lu,&nbsp;Jinyan Li,&nbsp;Ke Cheng,&nbsp;Guoning Zhu,&nbsp;Benzhong Zhu,&nbsp;Daqi Fu,&nbsp;Guiqin Qu,&nbsp;Yunbo Luo,&nbsp;Liqun Ma,&nbsp;Tao Lin,&nbsp;Chunjiao Zhang,&nbsp;Hua Huang,&nbsp;Hongliang Zhu","doi":"10.1111/tpj.70266","DOIUrl":"https://doi.org/10.1111/tpj.70266","url":null,"abstract":"<div>\u0000 \u0000 <p>Fruit cuticle, as a specialized hydrophobic cell wall architecture covering the surface of fruit, is crucial for fruit resistance to biotic and abiotic stress. In this study, we found that the BTB protein, SlBTA2, can regulate the biosynthesis of fruit cuticle in tomato. <i>SlBTA2</i> was mainly expressed in the epidermis of fruits. Knockout of <i>SlBTA2</i> inhibits the formation of fruit cuticle, resulting in a reduced cuticle thickness, accelerated post-harvest water loss, uneven colouring and increased cell wall thickness. GC-MS quantification revealed drastic reductions of cutin (94%) and wax (34%) monomer content in <i>slbta2</i> fruits, especially 9(10),16-dihydroxyhexadecanoic acid (&gt;90%). Moreover, transcriptome profiling identified coordinated downregulation of key cuticle biosynthesis genes in mutant fruits, such as <i>SlANL2b</i>, <i>SlLACS2</i>, <i>SlCER1-2</i>, etc. Overall, our findings present that <i>SlBTA2</i> is a novel breeding target for cuticle accumulation and post-harvest fruit qualities.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibiting reactive oxygen species production mitigates endoplasmic reticulum damage in florets of developing maize ears under heat stress","authors":"Huiqin Wang,&nbsp;Jing Sun,&nbsp;Hao Ren,&nbsp;Bin Zhao,&nbsp;Baizhao Ren,&nbsp;Jiwang Zhang,&nbsp;Zishan Zhang,&nbsp;Yuting Li,&nbsp;Yinglong Chen,&nbsp;Yakov Kuzyakov,&nbsp;Peng Liu","doi":"10.1111/tpj.70243","DOIUrl":"https://doi.org/10.1111/tpj.70243","url":null,"abstract":"<div>\u0000 \u0000 <p>Heat stress is increasingly becoming a major constraint to agricultural production due to global warming and higher probability of extreme events. To mitigate the yield loss caused by heat stress, it is essential to understand the mechanisms underlying its effects on young ear development. In this study, we investigate the impact of heat stress on heat-sensitive and heat-tolerant maize varieties under field conditions. A combination of phenotypic, physiological, anatomical, and multi-omics techniques was used to assess the properties of young ears, from the phenotypic to molecular level, in response to heat stress during growth. The results show that heat stress primarily disrupts endoplasmic reticulum function in maize. Specifically, heat stress disrupts mitochondrial structure, and abnormalities in the electron transport chain lead to an increase in reactive oxygen species (ROS) levels, resulting in oxidative stress, protein unfolding, and cellular structure disruption. Consequently, the fertilization rate of florets and the number of grains per ear decrease by 16%–42% and 33%–54%, respectively, resulting in a 29%–60% overall yield loss. ZD-tol (heat-tolerant variety) demonstrated thermotolerance by more rapidly activating various pathways, such as protein catabolism, energy metabolism, carbohydrate metabolism, amino acid metabolism, and lipid metabolism, raising the threshold for stimuli detection and accelerating cellular ROS detoxification. Compared to ZD-tol, XY-sens (heat-sensitive variety) exhibits weaker cellular detoxification ability, thereby demonstrating heightened sensitivity to heat stress. However, the application of ROS inhibitors significantly reduces ROS levels in florets, alleviate endoplasmic reticulum stress, and decreased yield loss by 17%–31%, with XY-sens showing better mitigation effects compared to ZD-tol.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144219916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NAP-dependent crosstalk between ethylene biosynthesis and abscisic acid signaling pathway coordinately modulates leaf senescence in plants","authors":"Lanxin Ma,&nbsp;Qian Gao,&nbsp;Yongbin Liu,&nbsp;Shun He,&nbsp;Haiying Xiang,&nbsp;Mingzhu Wu,&nbsp;Xin Xu,&nbsp;Zhaopeng Luo,&nbsp;Hongguang Li,&nbsp;Jun Yang,&nbsp;Zhong Wang","doi":"10.1111/tpj.70245","DOIUrl":"https://doi.org/10.1111/tpj.70245","url":null,"abstract":"<div>\u0000 \u0000 <p>Abscisic acid (ABA) and ethylene signaling activate the NAC transcription factor NAP, accelerating plant leaf senescence. NAP promotes the synthesis of ABA and ethylene in turn. However, the crosstalk between ABA and ethylene in regulating leaf senescence, and whether NAP plays roles in mediating the crosstalk between these two hormones, still remains to be further clarified. This study identified the tobacco NtNAP and verified its conserved roles in promoting leaf senescence. ChIP assay, transactivation analysis, yeast one-hybrid assay, and electrophoretic mobility shift assay demonstrated that NtNAP directly bound to the promoters and activated the expression of <i>NtACS8a</i>, <i>NtACO4-6</i>, <i>NtPYL4b</i>, and <i>NtSnRK2.6b</i> genes <i>in vivo</i> and <i>in vitro</i>, leading to the production of ethylene and enhanced ABA signaling in tobacco. Blocking ethylene synthesis by AVG treatment or knockout of <i>ACS</i> and <i>ACO</i> delayed ABA-induced leaf senescence in both tobacco and Arabidopsis, while loss of function of PYL4 and SnRK2.6 relieved leaf senescence induced by ACC treatment, which together validated the crosstalk between ABA and ethylene in regulating leaf senescence. Moreover, ABA promoted the production of ethylene in a NAP-dependent manner, and blocking NAP-induced ethylene production relieved leaf senescence caused by ABA treatment in both tobacco and Arabidopsis. Meanwhile, NAP also mediated the enhancement of ABA signaling induced by ACC treatment. Our results revealed a new mechanism by which NAP promotes leaf senescence through synergistic ABA and ethylene signaling.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144219915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}