The Plant Journal最新文献

{"title":"PbNAC3 coordinates AsA generation and ABA biosynthesis to improve salt tolerance in pear","authors":"Feng Zhang,&nbsp;Yanyan Gao,&nbsp;Mingyuan Ma,&nbsp;Lun Li,&nbsp;Yuchen Wei,&nbsp;Lemin Fan,&nbsp;Zhihua Xie,&nbsp;Kaijie Qi,&nbsp;Juyou Wu,&nbsp;Shutian Tao,&nbsp;Shaoling Zhang,&nbsp;Xiaosan Huang","doi":"10.1111/tpj.70171","DOIUrl":"https://doi.org/10.1111/tpj.70171","url":null,"abstract":"<div>\u0000 \u0000 <p>In plants, dehydroascorbate reductase (DHAR) is one of the key enzymes in AsA generation during the AsA-GSH cycle, which helps maintain the normal metabolic level of AsA. However, the molecular mechanism of DHAR's response to salt stress is still unknown. Our experiments show a ping-pong mechanism, in which DHA is combined with free reductase DHAR, and free reductase DHAR is combined with GSH in the form of sulfenylation to promote AsA generation in response to salt stress. This mechanism is inhibited by H₂O₂-mediated sulfenylation modification. The overexpression of PbDHAR3 in pear callus and Arabidopsis plants alleviated salt-induced damage, while its silencing decreased salt tolerance in <i>Pyrus betulaefolia</i>. PbNAC3 can activate the expression of <i>PbDHAR3</i> by directly binding to the promoter. The overexpression of <i>PbNAC3</i> in pear callus improved salt tolerance, while silencing it reduced tolerance in <i>P. betulaefolia.</i> Overexpression of <i>PbNAC3</i> in Arabidopsis plants is able to adjust the trade-off between plant growth and salt stress. Higher expression levels of <i>NCEDs</i> or <i>PYLs</i>, and higher ABA content were observed under salt treatment. Further experiments demonstrate that PbNAC3 activates <i>PbNCED5</i> through interaction with <i>cis</i>-regulatory elements. Overall, our results show that PbNAC3 plays a critical role in salt stress response by targeting the promoters of <i>PbDHAR3</i> and <i>PbNCED5</i>, promoting AsA generation and ABA biosynthesis. This study will deepen our understanding of the mechanisms underlying the trade-offs between plant growth and stress tolerance and assist the development of stress-resistant, high-yield crops.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NAM and CUC3 boundary genes maintain shoot apical meristem viability and suppress the development of axillary shoot in rice seedlings","authors":"Jieru Li,&nbsp;Tianhui Zhong,&nbsp;Ruihan Xu,&nbsp;Zhongyuan Chang,&nbsp;Yayi Meng,&nbsp;Chenyu Rong,&nbsp;Xi'an Shi,&nbsp;Yanfeng Ding,&nbsp;Chengqiang Ding","doi":"10.1111/tpj.70170","DOIUrl":"https://doi.org/10.1111/tpj.70170","url":null,"abstract":"<div>\u0000 \u0000 <p>Cell division and differentiation within the shoot apical meristem (SAM) are essential for the morphogenesis of aboveground plant organs. This study reveals that the boundary genes <i>OsNAM</i> and <i>OsCUC3</i> collaboratively maintain SAM activity. Loss of function in both <i>OsNAM</i> and <i>OsCUC3</i> during the fourth leaf stage reduced SAM size, with the <i>osnam oscuc3</i> mutant exhibiting abnormal leaf number and morphology. Furthermore, <i>OsNAM</i> and <i>OsCUC3</i> inhibited the growth of axillary shoots. In the <i>osnam oscuc3</i> mutant, the number of new leaves decreased, while buds in the coleoptile and the axil of the first leaf developed into tillers. Since <i>OsNAM</i> and <i>OsCUC3</i> are involved in regulating both SAM activity and the growth of lateral shoots, we examined their expression patterns at the base of the main shoot. β-Glucuronidase (GUS) reporter activity and GFP reporter lines demonstrated that <i>OsNAM</i> and <i>OsCUC3</i> have distinct expression patterns. Specifically, <i>OsNAM</i> was expressed throughout the SAM, whereas <i>OsCUC3</i> was expressed only at the base of the SAM, with its expression gradually decreasing as seedlings develop. RNA sequencing analysis showed that the expression of genes related to leaf epidermal cell development, cell wall components, and hormonal signal transduction was altered in response to the loss of function of <i>OsNAM</i> and <i>OsCUC3</i>. Therefore, the boundary genes <i>OsNAM</i> and <i>OsCUC3</i> not only inhibit the growth of axillary shoots but also regulate the development of aboveground organs, including leaf morphology and number, by maintaining the SAM activity in the main shoot.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 2","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time-lapse imaging establishes a roadmap for Brassica microspore embryogenesis","authors":"Gwendolyn K. Kirschner","doi":"10.1111/tpj.70168","DOIUrl":"https://doi.org/10.1111/tpj.70168","url":null,"abstract":"&lt;p&gt;Microspore embryogenesis describes a process whereby the haploid cell that usually develops into pollen is reprogrammed to become an embryo. For that, microspores are dissected from developing anthers and cultured &lt;i&gt;in vitro&lt;/i&gt;. Abiotic stress, such as heat treatment, can trigger their development into haploid embryos. The chromosome number of the haploid embryos can then be doubled, either spontaneously or chemically, to produce diploid (‘doubled-haploid’) plants with two sets of chromosomes. This results in homozygous diploid plants, as each chromosome in the haploid state is replicated. Having homozygous plants (i.e. genetic stability) available early in a breeding program significantly enhances breeding efficiency (Hale et al., &lt;span&gt;2022&lt;/span&gt;). Microspore embryogenesis of oilseed rape (&lt;i&gt;Brassica napus&lt;/i&gt;) has been studied since the 1980s, and the induction treatment is simple and short (Lichter, &lt;span&gt;1982&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;Charlotte Siemons, first author of the highlighted publication and a PhD student in Kim Boutilier's group at Wageningen University &amp; Research at the time of the study, was fascinated by this remarkable plasticity of plant cells. For Siemons, the ability of the microspore to switch cell fate from developing into mature pollen to forming an embryo provided an exciting opportunity to explore plant cell totipotency.&lt;/p&gt;&lt;p&gt;The application of heat stress to microspore cultures induces &lt;i&gt;B. napus&lt;/i&gt; microspores to develop into four distinct types of embryogenic tissue (Li et al., &lt;span&gt;2014&lt;/span&gt;). Two are differentiated embryos, either with or without a suspensor, while the other two are either compact or loose embryogenic calli. Both embryo types show high viability in culture and can develop into seedlings, but embryogenic calli have a low viability and generally never develop into differentiated embryos (Corral-Martínez et al., &lt;span&gt;2020&lt;/span&gt;). Currently, these tissue types can only be identified after about 5 days in culture, making it impossible to deduce the cell division dynamics leading to the different developmental pathways. To address this, Siemons &lt;i&gt;et al&lt;/i&gt;. used time-lapse imaging of &lt;i&gt;B. napus&lt;/i&gt; microspores to monitor the development of embryogenic structures from the single- to few-cell stage, allowing them to trace the cell divisions that lead to the formation of the different embryo types (Siemons et al., &lt;span&gt;2025&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;For the study, Boutilier's group teamed up with John van Noort's group at the University of Leiden to combine their expertise in &lt;i&gt;in vitro&lt;/i&gt; biology with John's expertise in high-resolution live imaging. Previously, they had used time-lapse imaging with confocal microscopy, but it negatively affected embryo development, most likely due to photo-induced damage. Two-photon microscopy, however, resulted in less photodamage due to the reduced absorption in near-infrared light when relatively low light doses were used, which allowed for long-term time-lapse imagi","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70168","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted metabolomics reveals anion and organ-specific metabolic responses of salinity tolerance in willow","authors":"Eszter Sas,&nbsp;Adrien Frémont,&nbsp;Emmanuel Gonzalez,&nbsp;Mathieu Sarrazin,&nbsp;Simon Barnabé,&nbsp;Michel Labrecque,&nbsp;Nicholas James Beresford Brereton,&nbsp;Frédéric Emmanuel Pitre","doi":"10.1111/tpj.70160","DOIUrl":"https://doi.org/10.1111/tpj.70160","url":null,"abstract":"<p>Willows can alleviate soil salinisation while generating sustainable feedstock for biorefinery, yet the metabolomic adaptations underlying their tolerance remain poorly understood. <i>Salix miyabeana</i> was treated with two environmentally abundant salts, NaCl and Na₂SO₄, in a 12-week pot trial. Willows tolerated salts across all treatments (up to 9.1 dS m⁻¹ soil EC_e), maintaining biomass while selectively partitioning ions, confining Na⁺ to roots and accumulating Cl⁻ and <span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msubsup>\u0000 <mi>SO</mi>\u0000 <mn>4</mn>\u0000 <mrow>\u0000 <mn>2</mn>\u0000 <mo>−</mo>\u0000 </mrow>\u0000 </msubsup>\u0000 </mrow>\u0000 <annotation>$$ {mathrm{SO}}_4^{2-} $$</annotation>\u0000 </semantics></math> in the canopy and adapting to osmotic stress via reduced stomatal conductance. Untargeted metabolomics captured &gt;5000 putative compounds, including 278 core willow metabolome compounds constitutively produced across organs. Across all treatments, salinity drove widespread metabolic reprogramming, altering 28% of the overall metabolome, with organ-tailored strategies. Comparing salt forms at equimolar sodium, shared differentially abundant metabolites were limited to 3% of the metabolome, representing the generalised salinity response, predominantly in roots. Anion-specific metabolomic responses were extensive. NaCl reduced carbohydrates and tricarboxylic acid cycle intermediates, suggesting potential carbon and energy resource pressure, and accumulated root structuring compounds, antioxidant flavonoids, and fatty acids. Na₂SO₄ salinity triggered accumulation of sulphur-containing larger peptides, suggesting excess sulphate incorporation leverages ion toxicity to produce specialised salt-tolerance-associated metabolites. This high-depth picture of the willow metabolome underscores the importance of capturing plant adaptations to salt stress at organ scale and considering ion-specific contributions to soil salinity.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CsbHLH122/CsMYBS3-CsSUT2 contributes to the rapid accumulation of sugar in the ripening stage of sweet orange (Citrus sinensis)","authors":"Xiawan Zhai,&nbsp;Xinxin Yu,&nbsp;Zuolin Mao,&nbsp;Mengdi Li,&nbsp;Zeqi Zhao,&nbsp;Changle Cai,&nbsp;Bachar Dahro,&nbsp;Jihong Liu,&nbsp;Chunlong Li","doi":"10.1111/tpj.70156","DOIUrl":"https://doi.org/10.1111/tpj.70156","url":null,"abstract":"<div>\u0000 \u0000 <p>The sugar content serves as the fundamental metabolic component that determines both the flavor quality and the nutritional value of fruits. Nevertheless, the regulatory mechanism underlying the rapid accumulation of sugars during citrus fruit maturation remains elusive. In this study, we demonstrated that the expression level of sucrose transporter <i>CsSUT2</i> is increased during citrus fruit ripening and sugar accumulation. Functional assays confirmed that CsSUT2 is localized in the plasma membrane and exhibits sucrose transporter activity. Homologous and heterologous overexpression of <i>CsSUT2</i> in citrus juice sacs, calli, and tomato resulted in an increase in sugar content. Conversely, virus-induced gene silencing and RNAi-mediated silencing of <i>CsSUT2</i> led to a decrease in sugar levels in transgenic citrus tissues. We further identified CsMYBS3 as an upstream transcription factor that positively regulates the expression of <i>CsSUT2</i>. Transgenic evidence supported that the induction of sugar accumulation by CsMYBS3 depends on the transcript level of <i>CsSUT2</i>. Additionally, we found that CsbHLH122 physically interacts with CsMYBS3 to form a transcription factor complex, enhancing promoter transcriptional activity of <i>CsSUT2</i>. This study expands our understanding of the function and regulatory mechanism of sugar transporter in citrus, providing valuable insights for regulating sugar accumulation and quality control in citrus fruit.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modelling the quantitative effect of oxygen on the ageing of primed celery seeds","authors":"Steven P. C. Groot,&nbsp;Paul W. Goedhart,&nbsp;Deborah de Souza Vidigal,&nbsp;Jan Kodde","doi":"10.1111/tpj.70066","DOIUrl":"https://doi.org/10.1111/tpj.70066","url":null,"abstract":"<p>High seed quality is a prerequisite for profitable crop production, but quality declines by ageing during storage. Whereas effects of temperature and humidity are well known, there is limited knowledge on the effect of oxygen. Here, we report on the quantitative effect of oxygen on seed ageing. Primed seeds from celery (<i>Apium graveolens</i>) were used as a model, because of their relatively short shelf life. The seeds were stored for up to 7 years at combinations of four relative humidity levels (16, 33, 43 and 60% RH), four temperatures (5, 13, 20 and 30°C) and six oxygen levels (≈1, 5.2, 10, 21, 50 and 99% on volume basis). A strong effect of low oxygen levels was observed at all temperatures and the three lower humidity levels. Modelling the viability data revealed a linear double logarithmic relationship between the oxygen level and the storage time at which the seed lot viability declined to 50% (<i>p</i>₅₀). The models also showed that each halving of the oxygen level increased seed longevity by around 72%. This implies that reduction of the environmental oxygen level to a level below 1% increased the shelf life of the primed celery seeds by a factor of 11. For seeds pre-equilibrated at 60% RH, the effect of lowering the oxygen level below 21% was much less pronounced and even absent at 30°C. The large effect of low oxygen level during dry storage of seeds provides opportunities to prolong the shelf life of seeds. Options for practical application are discussed.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking bamboo's fast growth: Exploring the vital role of non-structural carbohydrates (NSCs)","authors":"Yu Chen,&nbsp;Meng Tang,&nbsp;Yu-Chen Song,&nbsp;Meisam Zargar,&nbsp;Mo-Xian Chen,&nbsp;Shu-Yan Lin,&nbsp;Fu-Yuan Zhu,&nbsp;Tao Song","doi":"10.1111/tpj.70147","DOIUrl":"https://doi.org/10.1111/tpj.70147","url":null,"abstract":"<div>\u0000 \u0000 <p>Bamboo is known for its fast growth, and non-structural carbohydrates (NSCs) play a pivotal role in bamboo's fast growth. Despite extensive research on bamboo's growth, the role of NSCs, especially the underlying molecular regulatory mechanisms, in bamboo's fast growth remains largely unexplored. By studying growth patterns in various bamboo species, it was found that NSCs are transferred from mature bamboo to young shoots, facilitating their fast growth. This review explores NSCs in bamboo, covering their content, distribution, storage, and enzyme activities. It examines NSCs' physiological roles, including mobilization, transport, and growth facilitation, and discusses potential molecular regulatory mechanisms. It also summarizes the gene expression patterns involved in NSC synthesis and metabolism during bamboo's fast growth. NSCs regulate genes related to sugar transport, cell division, energy metabolism, and cell wall synthesis, thereby regulating bamboo's fast growth. NSCs interact with hormone signaling networks. Lastly, winter drought and cold stress stimulate NSC storage and transport. These stressors potentially serve as signals or prerequisites for NSC transport and accumulation. In general, this review summarizes the research progress on NSC transport from bamboo and its impact on bamboo's fast growth, providing a foundation for enhancing understanding and investigation of bamboo's fast growth mechanisms.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome wide association study of rice agronomical traits and seed ionome with the NARO Open Rice Collection","authors":"Nobuhiro Tanaka,&nbsp;Yoshihiro Kawahara,&nbsp;Kaworu Ebana,&nbsp;Matthew Shenton","doi":"10.1111/tpj.70152","DOIUrl":"https://doi.org/10.1111/tpj.70152","url":null,"abstract":"<div>\u0000 \u0000 <p>To meet the nutritional needs of the rising human population, genetic variants are necessary for the breeding of new cultivars. Rice (<i>Oryza sativa</i> L.) is a staple food for over half of the world's population. Here, we developed a new rice genetic resource, the NARO Open Rice Collection (NRC) with high-resolution genome data. NRC consists of 623 accessions, and approximately 200 accessions are categorized into three major subgroups, categorized as Indica, Japonica, and Aus. In this study, we performed genome-wide association studies (GWAS) for rice heading date, seed shape, and seed ionome using the NRC. Well-known genes related to heading date and seed shape were detected by GWAS using the NRC accessions. Therefore, we concluded that our new rice collection is suitable for GWAS. In addition, GWAS with each subgroup was advantageous for the detection of particular genes. Finally, we performed GWAS for seed ionome with the aim of improving the nutritional properties of rice, as essential minerals for humans, such as iron (Fe) and zinc (Zn), are not sufficient in rice seeds. Our study revealed that OsATL31, a likely ubiquitin E3 ligase, was involved in the control of Fe and Zn contents in seeds.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsMYB103 targets and interacts with AMD1 to form a feed-forward loop for pollen development in rice","authors":"Ting Zou,&nbsp;Qiuyu Ye,&nbsp;Liuhui Lu,&nbsp;Shiyue Xin,&nbsp;Rui Wang,&nbsp;Pingping Xiong,&nbsp;Qiao Li,&nbsp;Xin Zhang,&nbsp;Chuanhua Huang,&nbsp;Liu Tan,&nbsp;Ji Wang,&nbsp;Qiaoyi Tang,&nbsp;Xingzhe Xu,&nbsp;Jinghua Jin,&nbsp;Shiquan Wang,&nbsp;Qiming Deng,&nbsp;Yueyang Liang,&nbsp;Jun Zhu,&nbsp;Ping Li,&nbsp;Shuangcheng Li","doi":"10.1111/tpj.70164","DOIUrl":"https://doi.org/10.1111/tpj.70164","url":null,"abstract":"<div>\u0000 \u0000 <p>The process of male reproductive development in plants involves a series of biological events. These events are governed by complex transcriptional regulatory networks, which are composed of numerous transcriptional regulatory components (TRCs). ABERRANT MICROSPORE DEVELOPMENT1 (AMD1) is a recently identified TRC that is essential for rice pollen production. Nevertheless, the molecular roles of AMD1 in regulating rice pollen production are largely unknown. Here, we demonstrate that <i>AMD1</i> is directly activated by OsMYB103 as a genetic downstream target during rice pollen development. Interestingly, several direct target genes of OsMYB103, including <i>AMD1</i> itself, exhibit a more drastic expression down-regulation in the double mutant of <i>AMD1</i> and <i>OsMYB103</i> than in the single mutants. Our ongoing <i>in vivo</i> and <i>in vitro</i> experiments consistently indicate that AMD1 physically interacts with OsMYB103, thereby facilitating the activation of OsMYB103 on its target genes, possibly through the recruitment of the RNA polymerase II complex component OsTFIIF2-2. Our findings collectively suggest that the transcriptional cascade composed of OsMYB103 and AMD1 precisely regulates rice male fertility through a feed-forward loop mechanism, offering new insights into the role of AMD1 within the transcriptional regulatory network governing rice pollen development.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pan-analysis of intra- and inter-species diversity reveals a group of highly variable immune receptor genes in rice","authors":"Zhixue Wang,&nbsp;Yunxin Fan,&nbsp;Jiazhen Sun,&nbsp;Shaoyu Ma,&nbsp;Zhengwei Wang,&nbsp;Jiawen Li,&nbsp;Kunquan Liu,&nbsp;Ziwei Xiong,&nbsp;Changqing Li,&nbsp;Dongyuan Wang,&nbsp;Hongsheng Zhang,&nbsp;Jian Hua,&nbsp;Yongmei Bao","doi":"10.1111/tpj.70163","DOIUrl":"https://doi.org/10.1111/tpj.70163","url":null,"abstract":"<div>\u0000 \u0000 <p>Plant immune receptors and their natural variations play a central role in combating disease-causing pathogens. These immune receptors include intracellular nucleotide-binding leucine-rich repeat (LRR) receptors (NLRs) and cell-surface pattern recognition receptors (PRRs) that can be further classified as receptor-like proteins (RLPs) and receptor-like kinases (RLKs). Although the NLRome has been characterized, the repertoire and extent of diversity of PRRome remain undetermined in rice. In this study, we examined the diversity of immune receptor genes using high-quality genomes of 309 rice accessions from 8 species within the genus <i>Oryza</i>. A total of 376 310 immune receptor genes were identified, including 149 592 NLR-coding genes and 226 718 PRR coding genes. Shannon entropy analysis revealed a set of immune receptors that display significant intra-species and inter-species diversity in rice. In general, RLPs are more variable than RLKs, while NLRs and LRR-RLPs are more variable than LRR-RLKs. Additionally, NLR and PRR genes exhibit contrasting shoot/root expression patterns, with NLRs generally skewed towards root expression. Furthermore, we found that the size of the LRR-RLK gene families correlates with local annual precipitation, suggesting a stronger selection pressure on LRR-RLK genes in rice accessions grown under wet conditions than dry conditions. In sum, this pan-genomic analysis not only reveals the extensive diversity of the immune receptor repertoires in rice but also provides potential target genes for improving disease resistance in rice.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}