Proteomic snapshot of pattern triggered immunity in the Arabidopsis leaf apoplast

IF 5.7 1区 生物学 Q1 PLANT SCIENCES
Hsiao-Chun Chen, Carter J. Newton, Gustavo Diaz, Yaochao Zheng, Feng Kong, Yao Yao, Li Yang, Brian H. Kvitko
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Abstract

The apoplast is a critical interface in plant–pathogen interactions, particularly in the context of pattern-triggered immunity (PTI), which is initiated by recognition of microbe-associated molecular patterns. Our study characterizes the proteomic profile of the Arabidopsis apoplast during PTI induced by flg22, a 22-amino-acid bacterial flagellin epitope, to elucidate the output of PTI. Apoplastic washing fluid was extracted with minimal cytoplasmic contamination for liquid chromatography–tandem mass spectrometry analysis. By comparing our data to publicly available transcriptome profiles of flg22 treatment from 1 to 18 h, we observed that several highly abundant proteins exhibit relatively unchanged gene expression across all time points. We also observed topological bias in peptide recovery of 19 enriched receptor-like kinases with peptides predominantly recovered from their ectodomains. Notably, tetraspanin 8, an exosome marker, was enriched in PTI samples. We additionally confirmed increased concentrations of exosomes during PTI. This study enhances our understanding of the proteomic changes in the apoplast during plant immune responses and lays the groundwork for future investigations into the molecular mechanisms of plant defense under recognition of pathogen molecular patterns.

Abstract Image

拟南芥叶片外质体模式触发免疫的蛋白质组学快照。
外质体是植物与病原体相互作用的关键界面,特别是在模式触发免疫(PTI)的背景下,这是通过识别微生物相关的分子模式而启动的。我们研究了由22个氨基酸的细菌鞭毛蛋白表位flg22诱导的PTI过程中拟南芥外质体的蛋白质组学特征,以阐明PTI的输出。以最小的细胞质污染提取外质体洗涤液用于液相色谱-串联质谱分析。通过将我们的数据与公开可用的flg22处理1至18小时的转录组谱进行比较,我们观察到几个高度丰富的蛋白质在所有时间点上表现出相对不变的基因表达。我们还观察到19种富集的受体样激酶的肽恢复的拓扑偏差,其中肽主要从其外结构域恢复。值得注意的是,外泌体标记物tetraspanin 8在PTI样品中富集。我们还证实了PTI期间外泌体浓度的增加。本研究提高了我们对植物免疫应答过程中外质体蛋白质组学变化的认识,为进一步研究病原分子模式识别下植物防御的分子机制奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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