在拟南芥中,WRKY63的动态乙酰化受到HAG1和HDA6的拮抗调控

IF 5.7 1区 生物学 Q1 PLANT SCIENCES
Yuan-Hsin Shih, Pei-Yu Lin, Fu-Yu Hung, Keqiang Wu
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引用次数: 0

摘要

赖氨酸乙酰化是一个重要的翻译后修饰,调节蛋白质的功能、稳定性和亚细胞定位。虽然在哺乳动物系统中得到了广泛的研究,但它在植物中的作用在很大程度上仍未被探索。在本研究中,我们发现组蛋白乙酰转移酶HAG1和组蛋白去乙酰化酶HDA6是转录因子WRKY63乙酰化的关键拮抗调节因子。通过双分子荧光互补实验、分裂荧光素酶实验和共免疫沉淀实验,我们证明WRKY63与HAG1和HDA6相互作用。此外,WRKY63的n端区域对这些相互作用至关重要。hag1介导的乙酰化增强了WRKY63的核定位,而hda6介导的去乙酰化降低了WRKY63的核保留。此外,瞬时转录分析表明,HDA6抑制wrky63介导的转录激活。我们的研究结果强调了赖氨酸乙酰化在组蛋白修饰之外的更广泛意义,揭示了植物中调控转录因子活性的拮抗调控网络。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dynamic acetylation of WRKY63 is antagonistically regulated by HAG1 and HDA6 in Arabidopsis thaliana

Dynamic acetylation of WRKY63 is antagonistically regulated by HAG1 and HDA6 in Arabidopsis thaliana

Lysine acetylation is a crucial post-translational modification that regulates protein function, stability, and subcellular localization. While extensively studied in mammalian systems, its role in plants remains largely unexplored. In this study, we identify the histone acetyltransferase HAG1 and the histone deacetylase HDA6 as key antagonistic regulators of the transcription factor WRKY63 acetylation. Using bimolecular fluorescence complementation assays, split-luciferase assays, and co-immunoprecipitation assays, we demonstrate that WRKY63 interacts with HAG1 and HDA6. Furthermore, the N-terminal region of WRKY63 is essential for these interactions. HAG1-mediated acetylation enhances WRKY63 nuclear localization, whereas HDA6-mediated deacetylation reduces its nuclear retention. Moreover, transient transcriptional assays indicate that HDA6 represses WRKY63-mediated transcriptional activation. Our findings highlight the broader significance of lysine acetylation beyond histone modification, uncovering an antagonistic regulatory network that fine-tunes transcription factor activity in plants.

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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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