OsWRKY53 dictates wound responses in rice through fine-tuning crosstalk between PEP and PSK-mediated signalling

IF 5.7 1区 生物学 Q1 PLANT SCIENCES
Chitthavalli Y. Harshith, Dipasmit Palchaudhuri, Riju Dey, Steffi Raju, Padubidri V. Shivaprasad
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Abstract

Wounding is a major event during the multitude of stresses that plants face in their natural environment. Wound response is very dynamic and involves the integration of various regulatory networks culminating in successful wound-induced downstream signalling. Plants depend on endogenous molecular signals predominantly, small peptides, to initiate wound responses. Transcriptional response is paramount in dictating a successful wound response. Here we show the involvement of a WRKY transcription factor (TF) named OsWRKY53 that is upregulated upon wounding as well as wound-derived plant elicitor peptide (PEP), OsPep2 treatments. OsWRKY53 is involved in the positive regulation of gene expression of OsPep2-responsive genes. OsWRKY53 displays altered DNA occupancy in response to OsPep2 treatment over time, correlating with the altered gene expression. OsWRKY53 undergoes phosphorylation upon OsPep2 perception, and this contributes to the enhanced stability of the protein. Further, OsWRKY53 is involved in the simultaneous activation and suppression of OsPep2-responsive and phytosulfokine (PSK)-responsive genes, respectively. In agreement with these findings, perturbed expression of OsWRKY53 leads to compromised transcriptional responses post-wounding. Collectively, we establish that OsWRKY53 acts at the intersection of PEP and PSK-mediated transition of wound responses.

Abstract Image

OsWRKY53通过PEP和psk介导的信号传导之间的微调串扰决定水稻的伤口反应
受伤是植物在自然环境中面临的众多压力中的一个主要事件。伤口反应是非常动态的,涉及到各种调节网络的整合,最终在成功的伤口诱导的下游信号。植物主要依靠内源性分子信号,小肽,来启动伤口反应。转录反应在决定一个成功的伤口反应中是至关重要的。在这里,我们展示了一种名为OsWRKY53的WRKY转录因子(TF)的参与,该因子在损伤和伤口源性植物激发肽(PEP) OsPep2处理时上调。OsWRKY53参与ospep2应答基因表达的正向调控。随着时间的推移,OsWRKY53对OsPep2的处理表现出改变的DNA占用,这与改变的基因表达有关。OsWRKY53在OsPep2感知时发生磷酸化,这有助于增强该蛋白的稳定性。此外,OsWRKY53还参与同时激活和抑制ospep2应答基因和植物磺酰素(phytosulfokine, PSK)应答基因。与这些发现一致,OsWRKY53的表达紊乱导致损伤后转录反应受损。总之,我们确定OsWRKY53在PEP和psk介导的伤口反应转变的交叉点起作用。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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