LoZAT12通过激活东方百合杂种‘西伯利亚’中水杨酸和ABA的生物合成来加速花的衰老。

IF 5.7 1区 生物学 Q1 PLANT SCIENCES
Ziqiang Chen, Ruirui Li, Fengyuan Xu, Yiyi Yang, Cancan Yang, Jingjie Fang, Sitong Liu, Zhulong Chan, Linlin Zhong, Caiyun Wang, Fan Li, Guangfen Cui, Chunlian Jin, Shenchong Li, Jihua Wang, Hilary J. Rogers, Fan Zhang, Jing Luo
{"title":"LoZAT12通过激活东方百合杂种‘西伯利亚’中水杨酸和ABA的生物合成来加速花的衰老。","authors":"Ziqiang Chen,&nbsp;Ruirui Li,&nbsp;Fengyuan Xu,&nbsp;Yiyi Yang,&nbsp;Cancan Yang,&nbsp;Jingjie Fang,&nbsp;Sitong Liu,&nbsp;Zhulong Chan,&nbsp;Linlin Zhong,&nbsp;Caiyun Wang,&nbsp;Fan Li,&nbsp;Guangfen Cui,&nbsp;Chunlian Jin,&nbsp;Shenchong Li,&nbsp;Jihua Wang,&nbsp;Hilary J. Rogers,&nbsp;Fan Zhang,&nbsp;Jing Luo","doi":"10.1111/tpj.70465","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Flower senescence is an intrinsic developmental process in plants that is regulated by phytohormones, including salicylic acid (SA) and ABA. Senescence significantly influences the quality and duration of flower opening in ornamental plants, leading to the loss of their economic value. The C2H2-type zinc finger protein family is known to play a crucial role in plant stress responses; however, its involvement in the regulation of flower senescence has yet to be reported. In this study, LoZAT12, a <i>Lilium</i> oriental hybrid C2H2 zinc finger protein, was found to be a key regulator in SA- and ABA-mediated lily flower senescence. The expression of <i>LoZAT12</i> significantly increased during the late stage of flower opening, accompanied by the elevated levels of SA and ABA. Silencing of <i>LoZAT12</i> resulted in a delay of flower senescence, whereas overexpression of <i>LoZAT12</i> accelerated senescence in lily flowers, which was also confirmed in transgenic Arabidopsis. Furthermore, LoZAT12 was shown to directly bind to the promoters of SA and ABA biosynthesis-related genes, <i>LoPAL2</i> and <i>LoNCED3</i>, thereby activating their expression and enhancing the levels of SA and ABA. Additionally, LoZAT12 bound to the promoter of the protease gene <i>LoSAG39</i> and induced its expression, thereby facilitating lily flower senescence. The roles of <i>LoPAL2</i> and <i>LoNCED3</i> in promoting lily flower senescence were further confirmed through gene silencing. This study elucidates the dual-regulatory function of LoZAT12 in modulating the accumulation of SA and ABA, establishing a positive feedback loop that contributes to the coordinated flower senescence in lilies.</p>\n </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 6","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"LoZAT12 accelerates flower senescence by activating the biosynthesis of salicylic acid and ABA in Lilium oriental hybrid ‘Siberia’\",\"authors\":\"Ziqiang Chen,&nbsp;Ruirui Li,&nbsp;Fengyuan Xu,&nbsp;Yiyi Yang,&nbsp;Cancan Yang,&nbsp;Jingjie Fang,&nbsp;Sitong Liu,&nbsp;Zhulong Chan,&nbsp;Linlin Zhong,&nbsp;Caiyun Wang,&nbsp;Fan Li,&nbsp;Guangfen Cui,&nbsp;Chunlian Jin,&nbsp;Shenchong Li,&nbsp;Jihua Wang,&nbsp;Hilary J. Rogers,&nbsp;Fan Zhang,&nbsp;Jing Luo\",\"doi\":\"10.1111/tpj.70465\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Flower senescence is an intrinsic developmental process in plants that is regulated by phytohormones, including salicylic acid (SA) and ABA. Senescence significantly influences the quality and duration of flower opening in ornamental plants, leading to the loss of their economic value. The C2H2-type zinc finger protein family is known to play a crucial role in plant stress responses; however, its involvement in the regulation of flower senescence has yet to be reported. In this study, LoZAT12, a <i>Lilium</i> oriental hybrid C2H2 zinc finger protein, was found to be a key regulator in SA- and ABA-mediated lily flower senescence. The expression of <i>LoZAT12</i> significantly increased during the late stage of flower opening, accompanied by the elevated levels of SA and ABA. Silencing of <i>LoZAT12</i> resulted in a delay of flower senescence, whereas overexpression of <i>LoZAT12</i> accelerated senescence in lily flowers, which was also confirmed in transgenic Arabidopsis. Furthermore, LoZAT12 was shown to directly bind to the promoters of SA and ABA biosynthesis-related genes, <i>LoPAL2</i> and <i>LoNCED3</i>, thereby activating their expression and enhancing the levels of SA and ABA. Additionally, LoZAT12 bound to the promoter of the protease gene <i>LoSAG39</i> and induced its expression, thereby facilitating lily flower senescence. The roles of <i>LoPAL2</i> and <i>LoNCED3</i> in promoting lily flower senescence were further confirmed through gene silencing. This study elucidates the dual-regulatory function of LoZAT12 in modulating the accumulation of SA and ABA, establishing a positive feedback loop that contributes to the coordinated flower senescence in lilies.</p>\\n </div>\",\"PeriodicalId\":233,\"journal\":{\"name\":\"The Plant Journal\",\"volume\":\"123 6\",\"pages\":\"\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2025-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Plant Journal\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/tpj.70465\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/tpj.70465","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

花的衰老是植物内在的发育过程,受水杨酸和ABA等植物激素的调控。观赏植物的衰老对其开放质量和开放时间有显著影响,导致其经济价值的损失。已知c2h2型锌指蛋白家族在植物胁迫反应中起关键作用;然而,其参与调节花的衰老尚未报道。本研究发现东方百合杂交种C2H2锌指蛋白LoZAT12是SA-和aba介导的百合花衰老的关键调控因子。LoZAT12的表达在开花后期显著增加,同时SA和ABA水平升高。LoZAT12的沉默导致花的衰老延迟,而LoZAT12的过表达加速了百合的衰老,这在转基因拟南芥中也得到了证实。此外,LoZAT12直接结合SA和ABA生物合成相关基因的启动子LoPAL2和LoNCED3,从而激活它们的表达,提高SA和ABA的水平。此外,LoZAT12与蛋白酶基因LoSAG39的启动子结合,诱导其表达,从而促进百合花的衰老。通过基因沉默进一步证实了LoPAL2和LoNCED3在促进百合花衰老中的作用。本研究阐明了LoZAT12在调节SA和ABA积累中的双重调控功能,建立了一个正反馈回路,有助于百合花协调衰老。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

LoZAT12 accelerates flower senescence by activating the biosynthesis of salicylic acid and ABA in Lilium oriental hybrid ‘Siberia’

LoZAT12 accelerates flower senescence by activating the biosynthesis of salicylic acid and ABA in Lilium oriental hybrid ‘Siberia’

Flower senescence is an intrinsic developmental process in plants that is regulated by phytohormones, including salicylic acid (SA) and ABA. Senescence significantly influences the quality and duration of flower opening in ornamental plants, leading to the loss of their economic value. The C2H2-type zinc finger protein family is known to play a crucial role in plant stress responses; however, its involvement in the regulation of flower senescence has yet to be reported. In this study, LoZAT12, a Lilium oriental hybrid C2H2 zinc finger protein, was found to be a key regulator in SA- and ABA-mediated lily flower senescence. The expression of LoZAT12 significantly increased during the late stage of flower opening, accompanied by the elevated levels of SA and ABA. Silencing of LoZAT12 resulted in a delay of flower senescence, whereas overexpression of LoZAT12 accelerated senescence in lily flowers, which was also confirmed in transgenic Arabidopsis. Furthermore, LoZAT12 was shown to directly bind to the promoters of SA and ABA biosynthesis-related genes, LoPAL2 and LoNCED3, thereby activating their expression and enhancing the levels of SA and ABA. Additionally, LoZAT12 bound to the promoter of the protease gene LoSAG39 and induced its expression, thereby facilitating lily flower senescence. The roles of LoPAL2 and LoNCED3 in promoting lily flower senescence were further confirmed through gene silencing. This study elucidates the dual-regulatory function of LoZAT12 in modulating the accumulation of SA and ABA, establishing a positive feedback loop that contributes to the coordinated flower senescence in lilies.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信