Functional genetics of rice PISTILLATA genes reveals new roles and target genes in flowering time, female fertility, and parthenocarpy

IF 5.7 1区 生物学 Q1 PLANT SCIENCES
Mohamed Zamzam, Ritabrata Basak, Sharad Singh, Sandhan Prakash, Raghavaram Peesapati, Imtiyaz Khanday, Sara Simonini, Ueli Grossniklaus, Usha Vijayraghavan
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Abstract

Floral organ identity is controlled largely by the combinatorial action of MADS domain homeotic transcription factors. Lodicules are specialized plant organs in cereals and grasses that are involved in floret opening and facilitate pollination and fertility in rice (Oryza sativa L.). To understand the mechanisms underlying the specification of the rice lodicule, we investigated the developmental functions of the rice PISTILLATA (PI) paralogs, OsMADS2, and OsMADS4. Null osmads2 mutants reiterated OsMADS2 nonredundant lodicule specification roles and revealed new roles in flowering time and floral organ number and fate. Doubly perturbed osmads2 osmads4kd florets had severe abnormalities, were female infertile, yet could initiate parthenocarpy. Ubiquitous OsMADS4 overexpression rescued osmads2 abnormalities. We also utilized genome-wide binding analyses and transcriptome profiling to identify putative target genes contributing to OsMADS2 functions. In osmads2d8/d8 null mutant, we observed deregulated genes in a plethora of processes including lodicule and stamen development, floral organ number, and cell wall development. Some examples are cell division regulators (Cyclin D6, Cyclin-P4-1-like), an aquaporin (PIP1A), a peptide transporter, a vascular developmental regulator (HOX1), and a cell wall modulator (GH9B16). The deregulation of these genes may be associated with the disrupted cell division, tissue differentiation, and physiology of the malformed lodicules in osmads2 and osmads2 osmads4kd florets. Altogether, we reveal novel roles for the rice PI paralogs in flowering time, panicle exsertion, and embryo sac differentiation, identify gene targets for lodicule development, and provide mechanistic insights on the functional diversification of rice PI paralogs.

Abstract Image

水稻PISTILLATA基因的功能遗传学揭示了其在开花时间、雌性育性和孤雌结实中的新作用和靶基因
花器官的身份在很大程度上是由MADS结构域同源转录因子的组合作用控制的。小叶是谷类和禾本科植物的特殊器官,参与水稻小花的开放,促进授粉和生育。为了进一步了解水稻小泡调控的机制,我们研究了水稻pisttillata (PI)同源基因OsMADS2和OsMADS4的发育功能。osmads2零突变体重申了osmads2无冗余的小体规范作用,揭示了osmads2在开花时间、花器官数量和命运中的新作用。双扰动osmads2 osmads4kd小花有严重异常,雌性不育,但可以启动孤雌生殖。普遍存在的OsMADS4过表达挽救了osmads2异常。我们还利用全基因组结合分析和转录组分析来鉴定可能与OsMADS2功能有关的靶基因。在osmads2d8/d8零突变体中,我们观察到在包括小叶和雄蕊发育、花器官数量和细胞壁发育在内的大量过程中基因被解除调控。一些例子是细胞分裂调节剂(Cyclin D6, Cyclin- p4 -1样),水通道蛋白(PIP1A),肽转运蛋白,血管发育调节剂(HOX1)和细胞壁调节剂(GH9B16)。这些基因的失调可能与osmads2和osmads4kd小花的细胞分裂、组织分化和畸形小泡的生理紊乱有关。总之,我们揭示了水稻稻瘟病系在开花时间、穗外生和胚囊分化中的新作用,确定了水稻稻瘟病系发育的基因靶点,并为水稻稻瘟病系功能多样化的机制提供了新的见解。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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