{"title":"CsAPRR2通过协调叶绿体生物发生和光合作用相关基因的表达来调控黄瓜未成熟果实的颜色","authors":"Hanqiang Liu, Mingming Cui, Kaihong Hu, Junyi Tan, Yiqun Weng, Yuxuan Ma, Ju Li, Zeqiang Huang, Birong Chen, Huanwen Meng, Yupeng Pan, Zhihui Cheng","doi":"10.1111/tpj.70496","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Immature fruit color (IFC) of cucumber (<i>Cucumis sativus</i> L.) is an important marketability trait affecting consumer preferences. We previously identified a single-base insertion (−/G) in <i>CsAPRR2</i> that introduces a premature stop codon, leading to loss of the conserved C-terminal GCT box that is essential for protein–protein interactions. This mutation is strongly associated with reduced <i>CsAPRR2</i> expression and white IFC. RNA interference (RNAi) and transgenic overexpression confirmed the critical role of <i>CsAPRR2</i> in regulating IFC. Chromatin immunoprecipitation sequencing analysis and transcriptome profiling identified 225 candidate targets of CsAPRR2, many of which encode thylakoid membrane proteins or key enzymes in chlorophyll biosynthesis. Knockdown of <i>CsAPRR2</i> expression in RNAi plants significantly reduced the expression of these genes, resulting in impaired thylakoid development, lower chloroplast numbers, and reduced pigment content. CsAPRR2 physically interacts with transcription factors CsTCP15 and CsTCP20B in a GCT box–dependent manner, supporting a model in which these interactions contribute to <i>CsAPRR2</i>-mediated regulation of chloroplast development. Notably, a <i>CsTCP20B</i> variant identified in a wild cucumber with light green immature skin and white mature skin supports its involvement in skin color regulation. Together, this work establishes CsAPRR2 as a central regulator of chloroplast biogenesis and immature fruit pigmentation in cucumber and underscores the importance of transcription factor interactions in modulating photosynthesis-related gene expression.</p>\n </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 6","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CsAPRR2 regulates cucumber immature fruit color by coordinating chloroplast biogenesis and photosynthesis-related gene expression\",\"authors\":\"Hanqiang Liu, Mingming Cui, Kaihong Hu, Junyi Tan, Yiqun Weng, Yuxuan Ma, Ju Li, Zeqiang Huang, Birong Chen, Huanwen Meng, Yupeng Pan, Zhihui Cheng\",\"doi\":\"10.1111/tpj.70496\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Immature fruit color (IFC) of cucumber (<i>Cucumis sativus</i> L.) is an important marketability trait affecting consumer preferences. We previously identified a single-base insertion (−/G) in <i>CsAPRR2</i> that introduces a premature stop codon, leading to loss of the conserved C-terminal GCT box that is essential for protein–protein interactions. This mutation is strongly associated with reduced <i>CsAPRR2</i> expression and white IFC. RNA interference (RNAi) and transgenic overexpression confirmed the critical role of <i>CsAPRR2</i> in regulating IFC. Chromatin immunoprecipitation sequencing analysis and transcriptome profiling identified 225 candidate targets of CsAPRR2, many of which encode thylakoid membrane proteins or key enzymes in chlorophyll biosynthesis. Knockdown of <i>CsAPRR2</i> expression in RNAi plants significantly reduced the expression of these genes, resulting in impaired thylakoid development, lower chloroplast numbers, and reduced pigment content. CsAPRR2 physically interacts with transcription factors CsTCP15 and CsTCP20B in a GCT box–dependent manner, supporting a model in which these interactions contribute to <i>CsAPRR2</i>-mediated regulation of chloroplast development. Notably, a <i>CsTCP20B</i> variant identified in a wild cucumber with light green immature skin and white mature skin supports its involvement in skin color regulation. Together, this work establishes CsAPRR2 as a central regulator of chloroplast biogenesis and immature fruit pigmentation in cucumber and underscores the importance of transcription factor interactions in modulating photosynthesis-related gene expression.</p>\\n </div>\",\"PeriodicalId\":233,\"journal\":{\"name\":\"The Plant Journal\",\"volume\":\"123 6\",\"pages\":\"\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2025-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Plant Journal\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/tpj.70496\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/tpj.70496","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
CsAPRR2 regulates cucumber immature fruit color by coordinating chloroplast biogenesis and photosynthesis-related gene expression
Immature fruit color (IFC) of cucumber (Cucumis sativus L.) is an important marketability trait affecting consumer preferences. We previously identified a single-base insertion (−/G) in CsAPRR2 that introduces a premature stop codon, leading to loss of the conserved C-terminal GCT box that is essential for protein–protein interactions. This mutation is strongly associated with reduced CsAPRR2 expression and white IFC. RNA interference (RNAi) and transgenic overexpression confirmed the critical role of CsAPRR2 in regulating IFC. Chromatin immunoprecipitation sequencing analysis and transcriptome profiling identified 225 candidate targets of CsAPRR2, many of which encode thylakoid membrane proteins or key enzymes in chlorophyll biosynthesis. Knockdown of CsAPRR2 expression in RNAi plants significantly reduced the expression of these genes, resulting in impaired thylakoid development, lower chloroplast numbers, and reduced pigment content. CsAPRR2 physically interacts with transcription factors CsTCP15 and CsTCP20B in a GCT box–dependent manner, supporting a model in which these interactions contribute to CsAPRR2-mediated regulation of chloroplast development. Notably, a CsTCP20B variant identified in a wild cucumber with light green immature skin and white mature skin supports its involvement in skin color regulation. Together, this work establishes CsAPRR2 as a central regulator of chloroplast biogenesis and immature fruit pigmentation in cucumber and underscores the importance of transcription factor interactions in modulating photosynthesis-related gene expression.
期刊介绍:
Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community.
Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.