Zhen Lin, Jing Yang, Shengnan Liu, Yan Bai, Weihang Li, Jinsheng Lai, Weibin Song, Haiming Zhao, Qiujie Liu
{"title":"GT1通过影响茉莉酸途径调控玉米性别决定","authors":"Zhen Lin, Jing Yang, Shengnan Liu, Yan Bai, Weihang Li, Jinsheng Lai, Weibin Song, Haiming Zhao, Qiujie Liu","doi":"10.1111/tpj.70306","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Maize (<i>Zea mays</i> L.) is a monoecious plant with male and female flowers physically separated on different inflorescences—the tassel and the ear. Maize sex determination is controlled by a series of complicated developmental signals. Here, we characterized an EMS-induced maize feminized tassel mutant,<i>tasselsilk1</i> (<i>tsk1</i>), and identified <i>GRASSY TILLERS1</i> (<i>GT1</i>) as the causative gene. Phenotypic analysis of <i>tsk1</i> mutants revealed that pistils fail to abort in both the tassel and ear, resulting in long sterile silks in the tassel and the development of an extra small kernel from the lower floret in the ear. RNA-seq and CUT&Tag analysis indicated that GT1 functioned as a repressor for flower organ development by regulating the JA biosynthesis and signaling pathways, specifically by directly promoting the expression of <i>TASSELSEED1</i> (<i>TS1</i>), <i>ZmMYC2A</i>, <i>ZmMYC2B.</i> Together, we identified a new allele of <i>GT1</i> and proposed that GT1 functions through JA biosynthesis and signaling pathways to regulate sex determination in maize.</p>\n </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 1","pages":""},"PeriodicalIF":6.2000,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"GT1 regulates maize sex determination by affecting the jasmonate pathway\",\"authors\":\"Zhen Lin, Jing Yang, Shengnan Liu, Yan Bai, Weihang Li, Jinsheng Lai, Weibin Song, Haiming Zhao, Qiujie Liu\",\"doi\":\"10.1111/tpj.70306\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Maize (<i>Zea mays</i> L.) is a monoecious plant with male and female flowers physically separated on different inflorescences—the tassel and the ear. Maize sex determination is controlled by a series of complicated developmental signals. Here, we characterized an EMS-induced maize feminized tassel mutant,<i>tasselsilk1</i> (<i>tsk1</i>), and identified <i>GRASSY TILLERS1</i> (<i>GT1</i>) as the causative gene. Phenotypic analysis of <i>tsk1</i> mutants revealed that pistils fail to abort in both the tassel and ear, resulting in long sterile silks in the tassel and the development of an extra small kernel from the lower floret in the ear. RNA-seq and CUT&Tag analysis indicated that GT1 functioned as a repressor for flower organ development by regulating the JA biosynthesis and signaling pathways, specifically by directly promoting the expression of <i>TASSELSEED1</i> (<i>TS1</i>), <i>ZmMYC2A</i>, <i>ZmMYC2B.</i> Together, we identified a new allele of <i>GT1</i> and proposed that GT1 functions through JA biosynthesis and signaling pathways to regulate sex determination in maize.</p>\\n </div>\",\"PeriodicalId\":233,\"journal\":{\"name\":\"The Plant Journal\",\"volume\":\"123 1\",\"pages\":\"\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2025-07-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Plant Journal\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/tpj.70306\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/tpj.70306","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
GT1 regulates maize sex determination by affecting the jasmonate pathway
Maize (Zea mays L.) is a monoecious plant with male and female flowers physically separated on different inflorescences—the tassel and the ear. Maize sex determination is controlled by a series of complicated developmental signals. Here, we characterized an EMS-induced maize feminized tassel mutant,tasselsilk1 (tsk1), and identified GRASSY TILLERS1 (GT1) as the causative gene. Phenotypic analysis of tsk1 mutants revealed that pistils fail to abort in both the tassel and ear, resulting in long sterile silks in the tassel and the development of an extra small kernel from the lower floret in the ear. RNA-seq and CUT&Tag analysis indicated that GT1 functioned as a repressor for flower organ development by regulating the JA biosynthesis and signaling pathways, specifically by directly promoting the expression of TASSELSEED1 (TS1), ZmMYC2A, ZmMYC2B. Together, we identified a new allele of GT1 and proposed that GT1 functions through JA biosynthesis and signaling pathways to regulate sex determination in maize.
期刊介绍:
Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community.
Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.