CmCBF4 crosstalk with H2O2 signal involved in trehalose-promoted cold tolerance of melon seedlings

IF 5.7 1区生物学 Q1 PLANT SCIENCES

The Plant Journal Pub Date : 2025-10-15 DOI:10.1111/tpj.70520

Yuqing Han, Hongyi Zhang, Yujie Zhang, Lin Dong, Haiming Li, Jiahui Tian, Kexin Lu, Tao Liu, Hongyan Qi

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引用次数: 0

Abstract

Cold stress severely inhibits the normal growth of melon (Cucumis melo var. makuwa Makino) seedlings. At low temperature, the moderate increase of apoplastic hydrogen peroxide (H₂O₂) level and subsequent intracellular signal transduction are important to plant cold response. Our previous studies showed that trehalose (Tre) could activate H₂O₂ signal and improve cold tolerance of melon seedlings. However, the specific mechanism is not completely clear. Here, we found that Tre-promoted C-repeat binding factor 4 (CmCBF4) could combine with the promoter of respiratory burst oxidase homologs D (CmRBOHD) to enhance the production of apoplastic H₂O₂. Further studies suggested that CmCBF4 could also activate the transcription of plasma membrane intrinsic protein 2;3 (CmPIP2;3), which has H₂O₂ transport capability in melon seedlings. In addition, abscisic acid-responsive element (ABRE)-binding factor 2 (CmABF2) and CmCBF4 could promote the transcription of each other to increase the expression of CmPIP2;3. Silencing CmCBF4 significantly reduced Tre-promoted cold tolerance and apoplastic H₂O₂ production and transport. In summary, this study reveals that CmCBF4 regulates Tre-promoted cold tolerance in melon seedlings by promoting the production and transport of apoplastic H₂O₂ signal.

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CmCBF4与H2O2信号串扰参与海藻糖促进甜瓜幼苗抗寒性

冷胁迫严重抑制甜瓜（Cucumis melo var. makuwa Makino）幼苗生长。低温条件下，胞外过氧化氢（H2O2）水平的适度升高和随后的细胞内信号转导对植物的冷响应具有重要意义。我们的前期研究表明海藻糖可以激活H2O2信号，提高甜瓜幼苗的耐寒性。然而，具体机制尚不完全清楚。本研究发现，tre3促进的C-repeat结合因子4 （CmCBF4）可以与呼吸爆发氧化酶同源物启动子D （CmRBOHD）结合，促进外胞体H2O2的产生。进一步研究表明，CmCBF4还能激活质膜内禀蛋白2的转录；3 (CmPIP2;3)，在甜瓜幼苗中具有H2O2转运能力。2 .脱落酸反应元件（ABRE）结合因子2 （CmABF2）和CmCBF4可以相互促进转录，增加CmPIP2的表达；沉默CmCBF4显著降低了tre3促进的耐寒性和外胞体H2O2的产生和运输。综上所述，本研究揭示了CmCBF4通过促进外胞体H2O2信号的产生和运输来调节tre3促进的甜瓜幼苗抗寒性。

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来源期刊

The Plant Journal 生物-植物科学

CiteScore

13.10

自引率

4.20%

发文量

415

审稿时长

2.3 months

期刊介绍： Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.