生命科学最新文献

{"title":"Telomere-to-telomere genome assembly of the endangered Jacktree (Sinojackia xylocarpa Hu) reveals its centromeric landscape and evolutionary dilemma as a small population","authors":"Si-Di Liang,&nbsp;Si-Si Zheng,&nbsp;Lei Wu,&nbsp;Ang Liu,&nbsp;Hong-Hu Meng,&nbsp;Yun Jiang,&nbsp;Zheng-Wei Wang,&nbsp;Bin-Jie Ge,&nbsp;Wen-Li Zou,&nbsp;Qian Wan,&nbsp;Xi-Ling Dai,&nbsp;Gregor Kozlowski,&nbsp;Nai-Wei Li,&nbsp;Xiao-Ling Yan,&nbsp;Yi-Gang Song","doi":"10.1111/tpj.71186","DOIUrl":"10.1111/tpj.71186","url":null,"abstract":"<p>The endangered status of many plant species is often attributed to a decline in fitness, which drives a sustained decrease in population size under natural conditions. However, the role of highly repetitive genomic regions in the decline of endangered plants remains poorly understood. Here, we present the gap-free telomere-to-telomere (T2T) reference genome for the endangered Jacktree (<i>Sinojackia xylocarpa</i> Hu). It has a genome size of 961.94 Mb, an N50 of 78.05 Mb, and 36 446 predicted protein-coding genes. Using CENH3 chromatin immunoprecipitation sequencing (ChIP-seq), we identified centromeric regions across all 12 chromosomes and revealed that <i>S. xylocarpa</i> centromeres are composed of diverse transposable element (TE) families, with long terminal repeat (LTR) retrotransposons and LINE elements as major components. Notably, the right arm of chromosome 1 exhibited ectopic CENH3 enrichment and a TE accumulation similar to that of centromeric regions, suggesting the formation of a centromere-like chromatin domain. In addition, we detected inversion polymorphisms and inversion heterozygotes in natural populations, predominantly concentrated on the same chromosomal arm. These features may be associated with meiotic abnormalities. Using a high-quality T2T genome assembly, we identified a pattern of genomic erosion facing these small populations characterized by local suppression of recombination linked LTR proliferation and by the accumulation of deleterious mutations in DNA repair genes. This genomic erosion creates an evolutionary dilemma that impairs the purging of deleterious alleles, ultimately threatening long-term adaptability and survival. This study highlights the utility of T2T genome assembly for the conservation of endangered plants.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13732550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis thaliana BOLA proteins, new actors in plant growth and iron homeostasis","authors":"Maël Taupin-Broggini,&nbsp;Anaïs Rodriguez-Euzet,&nbsp;Brigitte Touraine,&nbsp;Nathalie Berger,&nbsp;Julie Bals,&nbsp;Daniel Marino,&nbsp;Christian Dubos,&nbsp;Florence Vignols","doi":"10.1111/tpj.71196","DOIUrl":"10.1111/tpj.71196","url":null,"abstract":"<p>The universal iron–sulfur (Fe-S) clusters embedded in proteins support numerous fundamental biological processes. However, many proteins involved in Fe-S assembly machineries remain poorly characterized in plants. Here, we revealed that <i>BOLA1</i> and <i>BOLA4,</i> two members of the BOLA family previously described as Fe-S assembly factors in various organisms, are critical growth regulators that play a role in Fe homeostasis in <i>Arabidopsis thaliana</i>. Expression analyses showed that <i>BOLA1</i> and <i>BOLA4</i> are ubiquitously expressed across most Arabidopsis tissues. Using protoplast transfection assays, we also demonstrated that both BOLA-GFP fusion proteins localize to chloroplasts and mitochondria. Mutant analyses revealed that while single <i>bola1</i> or <i>bola4</i> knock-out mutants are phenotypically indistinguishable from the wild type, double <i>bola1 bola4</i> mutants display severe growth defects, including early growth arrest of the primary root. Notably, this phenotype was rescued by grafting a wild-type root system onto the <i>bola1 bola4</i> aerial part. Fe quantification, Perls staining, and RT-qPCR analyses further demonstrated that <i>bola1 bola4</i> double mutants exhibit altered Fe distribution and Fe-homeostasis-related gene expression. Overall, our findings show that <i>BOLA1</i> and <i>BOLA4</i> act redundantly to maintain primary root growth and rosette development and are essential players in Fe signaling and partitioning in Arabidopsis.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71196","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STAG Priming Preserves Paneth Cells and Limits Parasite-Triggered Ileitis.","authors":"Ester Cristina Borges Araujo, Luciana Alves Sousa, Marisol Pallete Briceno, Loyane Bertagnolli Coutinho, Alexsandra Alves Bezerra Martins, Anne Fenton, Sumathi Sankaran-Walters, Paulo Czarnewski, Neide Maria Silva","doi":"10.1111/imcb.70173","DOIUrl":"https://doi.org/10.1111/imcb.70173","url":null,"abstract":"<p><p>Toxoplasma gondii triggered ileitis is a commonly used model for diseases affecting the small intestine, such as Crohn's Disease. Inflammation is primarily dependent on Th1-mediated killing of Paneth cells, which consequently promotes intestinal dysbiosis and boosts inflammation and pathology in the organs. Priming of the immune system with soluble Toxoplasma antigen (STAG) has been shown to induce immune regulation in several inflammatory diseases. Herein, we describe how protection of Paneth cells by priming with STAG is associated with protection against T. gondii triggered ileitis. We observed that STAG priming diminished intestinal pathology and modulated cytokine profile, and specifically downregulates interferon expression. Consequently, priming with STAG sustained Paneth-cell numbers during infection, unlike non-immunized controls, and thus also retained antimicrobial protein expression. Interestingly, we show that naturally resistant BALB/c mice did not undergo total Paneth-cell ablation, but upon additional Paneth-cell depletion, presented intestinal pathology and tissue parasitism levels similar to susceptible C57BL/6. Finally, we show that the STAG-primed groups presented a different intestinal microbiota, primarily through promotion of a protective microbiota enriched in Firmicutes and depleted of Enterobacteriaceae. Thus, the results suggest that priming with STAG is able to prevent Paneth-cell depletion and is associated with resistance to T. gondii triggered ileitis.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GmRVE8a, a circadian clock-associated gene, negatively regulates flowering and maturity in soybean","authors":"Shibo Sun,&nbsp;Maoxiang Yang,&nbsp;Tingting Wu,&nbsp;Bingjun Jiang,&nbsp;Xuegang Sun,&nbsp;Hongchang Jia,&nbsp;Xiangyu Yao,&nbsp;Yanhui Sun,&nbsp;Qimeng Li,&nbsp;Peiguo Wang,&nbsp;Liwei Jiang,&nbsp;Chao Qin,&nbsp;Shan Yuan,&nbsp;Zhaoming Qi,&nbsp;Shi Sun,&nbsp;Huihui Li,&nbsp;Tianfu Han,&nbsp;Baiquan Sun","doi":"10.1111/tpj.71205","DOIUrl":"10.1111/tpj.71205","url":null,"abstract":"<p>Precise regulation of flowering time and maturity is critical for achieving high and stable soybean yields, and the circadian clock plays a central role in coordinating these developmental transitions. Soybean is a typical short-day crop with a pronounced photoperiodic response; however, the molecular mechanisms linking circadian regulation to photoperiod-dependent flowering and maturity remain incompletely understood. Here, we integrated genome-wide association analysis with deep learning approaches using 257 soybean germplasm accessions spanning 14 maturity groups and identified <i>GmRVE8a</i>, a homolog of the Arabidopsis circadian clock gene <i>REVEILLE</i>, as a strong candidate locus associated with soybean maturity across two field environments. Haplotype analysis demonstrated that accessions carrying the <i>GmRVE8a</i>^<i>H1</i> haplotype consistently flowered and matured significantly later than those carrying <i>GmRVE8a</i>^<i>H2</i>. Functional validation revealed that knockout of <i>GmRVE8a</i> markedly accelerated flowering and maturity, whereas overexpression of <i>GmRVE8a</i> significantly delayed both processes. qRT-PCR assays further suggested that <i>GmRVE8a</i> may repress flowering and delay maturity by promoting the expression of <i>GmFT4</i>, a flowering-inhibitory member of the <i>FT</i> family, and is unlikely to act by directly regulating <i>E1</i> expression. Collectively, our findings suggest <i>GmRVE8a</i> as a circadian clock-associated negative regulator of soybean flowering and maturity, and highlight it as a promising candidate for molecular breeding aimed at improving adaptation to diverse photoperiodic environments.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13732551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population genomics of heterosporous lycophytes: allopatric speciation and historical persistence through paleoclimatic fluctuations","authors":"TongXin Ye,&nbsp;Ran Wei,&nbsp;JiGao Yu,&nbsp;NingXin Wang,&nbsp;SiQi Liang,&nbsp;XianChun Zhang","doi":"10.1111/tpj.71179","DOIUrl":"10.1111/tpj.71179","url":null,"abstract":"<p>Speciation is the fundamental process driving the origin of earth biodiversity. While speciation mechanisms have been revealed in many plant taxa, the evolutionary dynamics of heterosporous lycophytes remain largely unexplored. Here, we used the <i>Selaginella sanguinolenta</i> group, comprising seven montane species in eastern Asia, as a model system. We generated a high-quality, chromosome-level reference genome for <i>S</i>. <i>aitchisonii</i>, conducted whole-genome resequencing of 191 individuals across 49 populations, and integrated population genomic, phylogenomic, morphological, and ecological analyses. Our results revealed that strong geographic isolation, driven by Miocene paleogeographic events, led to high genetic differentiation and restricted interspecific gene flow. The unique combination of heterospory and independent gametophyte generations might exacerbate extreme inbreeding, further impeding the ability of these lineages to surmount geographical barriers. Ecological niche divergence indicated that these species experience heterogeneous selection pressures; moreover, evidence of selective sweeps, leaf arrangement variation, and functional gene divergence indicates that natural selection gives rise to new species with environmentally adaptive traits. Demographic reconstructions revealed a shared trajectory of expansion followed by contraction into bottlenecks from the late Miocene to the Pliocene. Montane refugia likely played a role in species persistence, preserving genetic diversity for the postglacial expansions observed in four species since the Last Interglacial. We conclude that the <i>S. sanguinolenta</i> group underwent allopatric speciation since the Miocene and successfully persisted through severe Quaternary climatic fluctuations, which provides novel insights based on population genomic analysis into the evolution of early-diverging vascular plants.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two nearly complete genomes reveal the molecular mechanism underlying the significant fruit quality differences caused by bud sport between yellow and white-fleshed loquat (Eriobotrya japonica L.)","authors":"Ke Zhao,&nbsp;Jinquan Chao,&nbsp;Yangang Pei,&nbsp;Tao Lang,&nbsp;Guoliang Jiang,&nbsp;Shuxia Sun,&nbsp;Hongjiang Xie,&nbsp;Jing Li,&nbsp;Meiyan Tu,&nbsp;Dong Chen,&nbsp;Mingchun Liu,&nbsp;Ronggao Gong,&nbsp;Haiyan Song","doi":"10.1111/tpj.71212","DOIUrl":"10.1111/tpj.71212","url":null,"abstract":"<p>Bud sport is a common and stable somatic variation in perennial fruit trees. Loquat (<i>Eriobotrya japonica</i> L.) is an evergreen fruit tree that can be categorized into yellow-fleshed and white-fleshed types based on the flesh color. To uncover the genetic basis, we herein assembled two nearly complete genomes for a yellow-fleshed ‘Dongting’ loquat (TBY) and its white-fleshed bud sport (TBW). The final sizes of assemblies were 773.29 Mb and 728.86 Mb with contig N50 of 31.87 Mb and 28.63 Mb for TBY and TBW, respectively. Comparative genomics analysis indicated that the heterozygosity ratio of TBY was nearly two-fold higher than that of TBW (0.53% vs. 0.27%). Integrated multi-omics and molecular marker detection results revealed that homozygosity of the <i>EjPSY2A</i>^<i>d</i>/<i>EjPSY2A</i>^<i>d</i> allele is the cause of the change in flesh color from yellow to white in TBY. Additionally, <i>EjSUSs</i> (<i>−1</i>, <i>−2</i>, <i>−3</i>, <i>−12</i>) and <i>EjSPS3</i> might be the key genes responsible for the significant accumulation of hexoses and sucrose, respectively, in the flesh of TBY and TBW. Furthermore, high expression of <i>EjC4H8</i> and <i>Ej4CLs</i> (<i>−7</i>, <i>−10</i>, <i>−20</i>) in TBY might lead to the accumulation of more lignin in its flesh. Taken together, these findings provide valuable insights into the molecular mechanism differences between yellow- and white-fleshed loquat and highlight the significance of bud sport as a valuable approach for generating novel germplasm resources in fruit trees.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71212","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149331921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant Receptor-Like Kinase-Mediated Drought Stress Response Mechanism-From Root Sensing to Stomatal Regulation.","authors":"Lijuan Yao, Xuechen Wang, Guifang Zhang, Yaning Cui, Xiaojuan Li","doi":"10.1111/pce.71021","DOIUrl":"https://doi.org/10.1111/pce.71021","url":null,"abstract":"<p><p>Drought is a multifaceted environmental constraint that alters water dynamics across the soil-plant-atmosphere system. In order to survive under drought stress, plants must respond at several levels from cellular signalling to whole-plant hydraulic regulation. Although receptor-like kinases (RLKs) have been reported to play important roles in these responses, they are often studied within individual molecular pathways rather than as integrators of environmental and physiological processes. Here, we review RLK-mediated drought signalling organised across three interconnected spatial scales. At the root-soil interface, RLKs perceive osmotic and mechanical cues to regulate root architecture, aquaporin dynamics and apoplastic barrier formation, thereby tuning root hydraulic conductivity. Over long distances, RLK-peptide modules and reactive oxygen species waves transmit water-deficit signals from roots to shoots via xylem transport and cell-to-cell propagation, while RLK-dependent vascular differentiation preserves hydraulic safety. In leaves, RLKs modulate stomatal aperture through ion-channel regulation, ROS-calcium signalling, cell-wall mechanosensing and developmental reprogramming of stomatal density. Importantly, we highlight how these pathways operate across distinct temporal scales-from rapid electrochemical responses to long-term anatomical remodelling-and identify key unresolved questions, including how signalling specificity is achieved among redundant RLK members and how hydraulic and biochemical cues are integrated. By linking RLK-mediated signalling to whole-plant physiological responses, this review offers potential avenues for enhancing crop drought resilience through targeted modulation of RLK pathways.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bringing seed barriers into focus: Optimized clearing and histological staining","authors":"Fabien Nirina Ramamonjy,&nbsp;Luca Piccinini,&nbsp;Vanessa Reyes Loaiza,&nbsp;Hongjun Meng,&nbsp;Robertas Ursache","doi":"10.1111/tpj.71209","DOIUrl":"https://doi.org/10.1111/tpj.71209","url":null,"abstract":"<p>Plant reproduction relies on tightly coordinated developmental programs that integrate environmental and endogenous signals. Within seeds, lipid-based extracellular polymers such as cutin and suberin are deposited at defined apoplastic interfaces, where they function as selectively permeable barriers. These structures are actively remodeled by hormonal and metabolic cues to regulate hydration, nutrient exchange, and stress responses. Although the genetic and biochemical pathways underlying these barriers are increasingly well understood, their spatial organization and developmental dynamics remain difficult to investigate because they are embedded within optically dense and multilayered tissues. Existing clearing approaches have substantially improved visualization of internal seed structures, yet systematic whole-mount imaging of multiple lipid-based barriers across development, particularly in combination with fluorescent reporters, remains challenging. To address this, we developed an optimized pipeline that enables rapid and efficient seed tissue clearing and selective visualization of polyaliphatic barriers across four key seed interfaces: the seed surface, seed coat suberin, cuticle at the endosperm/seed coat boundary, and embryo surface cuticle. The method preserves the spatial context of these barriers within intact seeds and enables their co-visualization with fluorescence-based marker lines. Using a common whole-mount imaging framework across successive developmental stages, we follow changes in the spatial organization of distinct barrier domains. The approach is also applicable to more complex seeds, such as those of <i>Camelina sativa,</i> and can be extended to other anatomically complex plant organs by combining the clearing and staining workflow with vibratome sectioning.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"128 3","pages":""},"PeriodicalIF":6.2,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.71209","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149311440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant Phenotyping in Plant Factories: Progress, Challenges and Prospects.","authors":"Shumao Wang, Luyang Kang, Jigang Li, Rongcheng Lin","doi":"10.1111/pce.71001","DOIUrl":"https://doi.org/10.1111/pce.71001","url":null,"abstract":"<p><p>Plant factories with artificial lighting (PFALs) are enclosed vertical cultivation systems equipped with customisable LED spectral regulation, whose stacked spatial structure and controlled light environments substantially differ from open fields and conventional greenhouses, bringing unique and specialised challenges to precise plant phenotyping. Driven by high-throughput sensing technologies and artificial intelligence (AI), plant phenotyping in PFALs has achieved considerable advances. This review comprehensively summarises progress in PFALs plant phenotyping, covering sensing techniques including RGB imaging, multispectral imaging, hyperspectral imaging, and 3D point cloud reconstruction, as well as advanced analytical models integrating machine learning, deep learning, large language models, and AI agents for phenotypic data mining. We further highlight the important bottlenecks restricting the industrial application of PFALs phenotyping: commercial phenotyping devices are generally bulky, costly, and incompatible with compact vertical cultivation layouts, while the shortage of PFALs-specific phenotypic datasets and scenario-adaptive optimisation models hinders large-scale industrial translation. To clarify the differentiated technical characteristics of PFALs phenotyping, we systematically compare the discrepancies in phenotyping strategies, technical demands, and application scenarios between PFALs and traditional greenhouses. On this basis, we propose targeted solutions and prospective development directions for PFALs phenotyping, focusing on miniaturised and integrated phenotyping equipment, optimised AI-driven analytical frameworks, and multi-omics intelligent breeding. Distinct from previous general phenotyping reviews, this paper specifically targets the vertical, controlled PFALs cultivation scenario, systematically sorting out its exclusive technical challenges and corresponding coping strategies, and providing actionable insights for the future intelligent upgrading and industrialisation of PFALs / vertical farm plant phenotyping technology.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Tartary Buckwheat FtMYB15 Coordinates Cadmium Uptake and Detoxification by Regulating FtZIP3 and FtGSTU19 Expression.","authors":"Xinrong Zhang, Jianglan Wei, Xuan Tang, Anhu Wang, Yi Zhou, Sijie Mai, Yunqi Liu, Yi Yang, Xiaoyi Li, Jianmei Wang","doi":"10.1111/pce.71020","DOIUrl":"https://doi.org/10.1111/pce.71020","url":null,"abstract":"<p><p>Tartary buckwheat (Fagopyrum tataricum) is a crop of significant value, yet its cultivation in cadmium (Cd)-contaminated soils poses risks to food safety. Understanding the molecular mechanisms of Cd uptake is crucial for developing strategies to minimize its accumulation. Here, we demonstrate that the Tartary buckwheat transcription factor FtMYB15 promotes Cd²⁺ influx and enhances Cd accumulation in transgenic hairy roots. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assay (EMSA) results indicate that FtMYB15 binds to the MYB-binding sites (MBS) of the FtZIP3 promoter, thereby upregulating the expression of FtZIP3, which encodes a zinc/iron-regulated transporter-like protein. Overexpression of FtZIP3 similarly enhances Cd²⁺ influx and Cd accumulation in buckwheat hairy roots. Further analysis reveals that FtMYB15 binds to the MBS in the promoter of the tau glutathione S-transferase gene FtGSTU19 and upregulates its expression in buckwheat hairy roots both before and after Cd exposure. Overexpression of FtGSTU19 in buckwheat hairy roots enhances Cd detoxification capacity via its glutathione transferase activity, thereby alleviating Cd-induced oxidative stress. Heterologous expression of FtGSTU19 increases Cd tolerance in Arabidopsis. Expression of FtMYB15, FtZIP3, and FtGSTU19 is induced by Cd stress. Together, these findings demonstrate that FtMYB15 directly regulates the expression of FtZIP3 and FtGSTU19, thereby coordinating Cd uptake and detoxification in plants.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149316527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}