生命科学最新文献

{"title":"Algal origins of core land plant stress response subnetworks","authors":"Armin Dadras,&nbsp;Pauline Duminil,&nbsp;Sophie de Vries,&nbsp;Iker Irisarri,&nbsp;Ivo Feussner,&nbsp;Jan de Vries","doi":"10.1111/tpj.70291","DOIUrl":"https://doi.org/10.1111/tpj.70291","url":null,"abstract":"<p>We computed co-expression networks from more than 2200 samples of nine species across 600 million years of divergent streptophyte evolution and infer that the streptophyte algal ancestors of land plants already had a remarkable fraction of the embryophytic stress response system. Despite its phytohormone-independent origin, homologs of all core components of the drought hormone abscisic acid (ABA) subnetwork are present, and we find that most are co-expressed in streptophyte algae and land plants; this subnetwork was thus co-opted in embryophytes by bringing it under the regime of ABA. The last common ancestor of embryophytes and Zygnematophyceae algae had ancient stress-responsive pathways, enabling it to face the stresses typical of the land environment – even before the origin of land plants – while evolution on land led to the adaptive refinement of these responses.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70291","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fragmentation of nucleoli in senescent cancer cells is associated with increased levels of polyadenylated transcripts derived from noncoding regions of rDNA units.","authors":"Jana Sochorová, Emilie Lukášová, Eva Volfová Polanská, Kateřina Řehůřková, Aleš Kovařík","doi":"10.1007/s00249-025-01773-9","DOIUrl":"https://doi.org/10.1007/s00249-025-01773-9","url":null,"abstract":"<p><p>In this study, we investigated the behavior of rDNA loci in senescent MCF-7 mammary cancer cells induced by gamma irradiation. To analyze changes in nucleolar structure we used rDNA-FISH and immunohistochemical staining with fibrillarin and UBF transcription factor. The expression levels of rDNAs and nucleolar proteins were determined by RNA-seq of total and poly-A libraries. The cytological and molecular parameters of nucleoli were monitored throughout the 7-day interval following irradiation. Senescent cells exhibited a higher proportion of smaller nucleoli as compared to cycling cells, indicating nucleolar fragmentation. The rDNA copy number and expression of rDNA variants remained stable in cycling and senescent cells. However, the levels of polyadenylated rRNA species derived from external (5'ETS) and internal (ITS1) rDNA spacers tend to increase (c.2 fold) following irradiation. At the protein level, senescent cells showed decreased levels of fibrillarin and UBF transcription factor while localization of both proteins in the nucleolus was not impaired. We conclude that withdrawal from cell cycle does not change expression patterns of rDNA variants. However, defects in rRNA processing may lead to fragmentation of nucleoli in senescent cells.</p>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Observation of RNAs and Their Binding Proteins in Plant Cells.","authors":"Junfei Ma, Yunhan Wang, Svetlana Y Folimonova, Bin Liu, Ying Wang","doi":"10.1111/pce.70034","DOIUrl":"https://doi.org/10.1111/pce.70034","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"F-Actin Polarization and Microtubule Integrity Direct Regeneration Patterns and Polarity of Cell Outgrowth in Wound-Induced Reprogramming.","authors":"Yi-Ting Huang, Yun-Ching Yen, Joop E M Vermeer, Viola Willemsen, Han Tang","doi":"10.1111/pce.70032","DOIUrl":"https://doi.org/10.1111/pce.70032","url":null,"abstract":"<p><p>Plants have developed a high regenerative capacity to repair damaged tissues and regenerate new organs in response to injury. When wounded, cells detect mechanical forces through their cytoskeletons and transmit molecular signals to the nucleus, triggering cell reprogramming. As mechanosensing and cell reprogramming have been studied separately, the connection between these processes and the role of cytoskeletal networks in regeneration is still unclear. This study used Physcomitrium patens to investigate the spatiotemporal dynamics of actin filaments and microtubules during wound-induced cell reprogramming. Upon laser-induced wounding, we observed a rapid and localized accumulation of F-actin at the plasma membrane of the neighboring cells next to the wounding site, whereas microtubules showed no immediate discernible changes. Disruption of F-actin severely impaired overall regeneration, leading to significant reductions in the reprogramming rate. Perturbations of microtubules primarily impacted regenerative cell divisions. Depolymerization of cytoskeletal networks altered regeneration patterns, reflected in the higher ratio of cell outgrowth to division and the outgrowth polarity. These findings underscore the functional role of the cytoskeleton in regulating cell reprogramming. This study reveals that early cytoskeletal polarization after wounding guides the polarity of cell outgrowth, providing new insights into how plants regenerate from mechanical damage.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Rhizodeposition in the Woody Perennial Populus trichocarpa.","authors":"Manasa R Appidi, Sameer Mudbhari, Kevin Cope, Sara S Jawdy, Dana L Carper, Edanur Öksüz, Xianghu Wang, Timothy Tschaplinski, Mingxun Wang, Robert L Hettich, Udaya Kalluri, Paul E Abraham","doi":"10.1111/pce.70004","DOIUrl":"https://doi.org/10.1111/pce.70004","url":null,"abstract":"<p><p>Plants undergo physiological and metabolic changes that release specific molecules into the surrounding soil, a process collectively known as rhizodeposition. These compounds play crucial roles in plant-microbe-soil interactions, such as supporting plant development and resilience in changing environments. Under nutrient-limited conditions, these plant-derived compounds modify the rhizosphere environment, mobilizing otherwise inaccessible nutrients and recruiting stress-adaptive microbial communities that support stress resilience. Currently, the chemical diversity of rhizodeposition has yet to be fully realized but is expected to be a complex mixture that includes soluble organic compounds excreted from root cells, along with products of root cell turnover, sloughed-off root cap and border cells, and mucilage. Here, we developed a methodological and conceptual framework for an in-depth measurement of rhizodeposition through critical advancements in untargeted metabolomics. This approach provided foundational insights into the dynamic changes in rhizodeposition for the woody perennial Populus trichocarpa and rhizodeposit profiles varying by genotype, time, location, and environment. More broadly, this study provides a framework that will help formulate the next steps to effectively study rhizodeposition.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TurboID-Based Proximity Labelling Identifies MED25 as an Activator of GoPGF-Mediated Gland Formation and Gossypol Biosynthesis in Cotton.","authors":"Lu Long, Fu-Chun Xu, Fen Li, Zhen-Nan Zhang, Shen-Zhai Shang, Jing-Ruo Zhao, Gai-Yuan Hu, Jia-Yi Ma, Man Yuan, Jose Ramon Botella, Yingfang Zhu, Shuang-Xia Jin, Wei Gao","doi":"10.1111/pce.70026","DOIUrl":"https://doi.org/10.1111/pce.70026","url":null,"abstract":"<p><p>The pigment glands of cotton synthesize the toxic gossypol that contributes to cotton's defence, but also limits the usage of cottonseed as a food source. Previous studies have shown that the gland-localized GoPGF is the master transcription factor controlling the gland morphogenesis and gossypol biosynthesis in cotton. However, the precise mechanism underlying the GoPGF-mediated transcription of downstream genes remains unclear. In this study, TurboID-based proximity labelling was established in cotton using a transient expression system to identify the components of GoPGF-complex. A total of 48 high-confidence GoPGF-proximal proteins were identified, and a mediator subunit, MED25, was shown to control gland formation through phenotypic studies. Several lines of evidence indicate that GhMED25 physically interacts with GoPGF in the nucleus, and GhMED25-GoPGF complex regulates the expression of GhJUB1 related to gland morphogenesis, and several well-characterized gossypol biosynthetic genes. ChIP-qPCR further revealed that GhMED25 acts as an enhancer of the GoPGF-mediated transcriptional output. Our study shows the value and reliability of the proximity labelling system in studying the low-abundance and cell-type-specific proteins in crops, adding a new role to the multifunctionality of MED25 in plants, and provides new insights into the intricate signalling network governing gland formation and terpene production in cotton.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CaM4 Functions as a Positive Regulator of the SOS Pathway in Response to Salt Stress.","authors":"Jiaojiao Zhang, Xiaoying Li, Xiaotong Liu, Jirui Feng, Wenxu Wang, Xiaoyan Lu, Jian-Xiu Shang, Liqun Zhao","doi":"10.1111/pce.70021","DOIUrl":"https://doi.org/10.1111/pce.70021","url":null,"abstract":"<p><p>Salt poses a major environmental threat to plant growth and development. In Arabidopsis thaliana (Arabidopsis), salt overly sensitive (SOS) is a major Ca²⁺-activated salt-responsive signalling pathway defined by four main components: SOS1, SOS2, SOS3 and SOS3-like calcium-binding protein 8 (SCaBP8). Previously, we reported that a calmodulin (CaM) isoform, CaM4, functions in salt resistance by reestablishing the ion balance in Arabidopsis. Here, we showed that CaM4 directly binds to SOS2 (core element of the SOS pathway) both in vivo and in vitro and stimulates its kinase activity under salt stress. This finding was supported by the observation of CaM4 aggregation in the plasma membrane (PM) in response to NaCl. Genetic evidence has revealed that the overexpression of an activated SOS2 protein (SOS2^T168D or SOS2^DF) partially rescued the salt-sensitive phenotype of cam4 plants. Thus, SOS2 is considered a CaM4 target under saline conditions. Biological analyses have indicated that CaM4 activates SOS1 (a PM Na⁺/H⁺ antiporter) through SOS2 and enhances the interaction between SOS2 and SOS1, leading to the expulsion of excess Na⁺ from plant cells. A deficiency in CaM4 further reduced SOS2 kinase activity in scabp8sos3 double mutant plants. Thus, CaM4, together with SOS3 and SCaBP8, coregulate SOS2 kinase activity in Arabidopsis to promote its adaptation to salt stress. Collectively, our results show that CaM4 is a positive regulator of the SOS signalling pathway, which promotes plant salt tolerance.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genistein Exudation Drives Spatial Root Allocation and Heterogeneous Microbial Communities to Enhance Phosphorus Acquisition in Soybean-Maize Intercropping.","authors":"Bingbing Zhang, Huadong Jiang, Guitian Zheng, Zeyu Zhang, Tianqi Wang, Qianqian Chen, Xiaoyuan Chen, Xing Lu, Cuiyue Liang, Jiang Tian","doi":"10.1111/pce.70020","DOIUrl":"https://doi.org/10.1111/pce.70020","url":null,"abstract":"<p><p>Soybean-maize intercropping improves phosphate (Pi) acquisition in phosphorus (P) deficient soils through flavonoid-mediated plant-microbe interactions. Yet, the molecular mechanisms driving spatially heterogeneous root-microbe interactions mediated by secreted flavonoids remain unexplored. Using GmHAD1-2 suppression line (Ri) and wild-type (WT), we demonstrated that root-secreted flavonoids, particularly genistein, drive spatial differentiation of root allocation and rhizosphere microbial communities in intercropped soybean with maize, specifically under low-P conditions. Compared to WT, Ri reduced genistein secretion and restricted root allocation to the root non-interaction zone, thereby diminishing the intercropping advantage by less shoot biomass and P uptake. In all cropping systems, WT in intercropping recruited Bacillus in root non-interaction zones, while Pseudomonas in root interaction zones. Furthermore, inoculation experiments demonstrated their synergistic roles. Bacillus stimulated root elongation and enhanced transcription of auxin-responsive genes (i.e., GmPIN2b and GmYUC2a), whereas Pseudomonas elevated Pi availability in rhizosphere soils and upregulated Pi transporters (i.e., GmPHF1 and GmPT4). Taken together, spatial root allocation and heterogeneous microbial communities across root zones play a critical role in determining intercropping advantages, which is regulated by genistein exudation in soybean roots. Our study provides novel insights into root exudate-driven microbial zonation as a strategic adaptation to nutrient stress, with implications for optimising sustainable intercropping systems.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From data to discovery: leveraging big data in plant natural products biosynthesis research","authors":"Matthew McConnachie,&nbsp;Tuan-Anh Minh Nguyen,&nbsp;Truc Kim,&nbsp;Trinh-Don Nguyen,&nbsp;Thu-Thuy T. Dang","doi":"10.1111/tpj.70288","DOIUrl":"https://doi.org/10.1111/tpj.70288","url":null,"abstract":"<p>Plant natural products or specialized metabolites play a vital role in drug discovery and development, with many clinically important derivatives such as the anticancer drugs topotecan (derived from the natural alkaloid camptothecin) and etoposide (derived from the natural polyphenol podophyllotoxin). Remarkable advances in understanding plant natural product metabolism have been achieved at an unprecedented pace over the past 15 years. The integration of high-throughput technologies in genomics, transcriptomics, and metabolomics has generated vast datasets that provide a more comprehensive understanding of plant metabolism. Additionally, advances in computational tools, machine learning, and data analytics have played a crucial role in processing and interpreting the massive amounts of newly available data, enabling researchers to uncover intricate regulatory networks and identify key components of biosynthetic pathways. This review navigates the evolving landscape of plant biosynthetic pathway elucidation accelerated by innovative multidisciplinary strategies that capitalize on big data. We highlight recent advances in plant-specialized biosynthesis that illustrate how big data are increasingly leveraged to unravel the complexities of plant metabolism.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"122 6","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jasmonic Acid Activates Transcription Factor SlMYB13 to Enhance Cold Resistance in Tomato.","authors":"Ning Zhang, Haoxuan Zhang, Bing Bai, Jinzhe Li, Xia Cao, Xiujie Mao, Qun Liu, Chuncheng Wu","doi":"10.1111/pce.70030","DOIUrl":"https://doi.org/10.1111/pce.70030","url":null,"abstract":"<p><p>Plant cold stress caused by cold waves often occurs during winter and spring, and seriously affects the growth and yield of tomatoes in horticultural facilities. The cultivation of cold-resistant tomato varieties during winter is therefore of great significance. Methyl jasmonate (MeJA) treatment is known to improve tomato cold tolerance. Using RNA sequencing, the R2R3 MYB transcription factor SlMYB13 (Solyc06g083900) responsive to MeJA and cold stress was identified as a potential regulator in this process. However, its regulatory mechanisms remain unclear. To investigate the role of SlMYB13 in cold resistance of tomatoes, SlMYB13 overexpression and CRISPR-Cas9 knockout seedlings of T₂ generation tomato were treated at 4°C. Tomato plants overexpressing SlMYB13 were more cold-tolerant, whereas SlMYB13-knockout plants exhibited significantly increased sensitivity to cold. Notably, exogenous MeJA application significantly increased cold resistance in the knockout plant. In vitro and vivo analyses revealed that SlMYB13 directly binds to the promoter SlHSP17.7, a small heat shock protein gene involved in cold-stress response. SlMYB13 regulated the expression of SlMYC2, a key regulator of the jasmonic acid signalling pathway at transcription and protein levels, and SlMYC2 also directly binds to the promoter of SlHSP17.7. Collectively, these findings demonstrate that SlMYB13 promotes cold tolerance through SlMYC2-mediated jasmonic acid signalling targeting SlHSP17.7. This study provides valuable insights for the genetic improvement of cold-tolerant tomato cultivars.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}