Plant, Cell & Environment最新文献

Arbuscular Mycorrhizal Fungi Improve Rice Production in Zinc-Amended Soils by Altering Zinc Transport and Translocation Routes. 丛枝菌根真菌通过改变锌的运输和转运途径提高锌改良土壤中的水稻产量。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-04-09 DOI: 10.1111/pce.15543

Yang Zhang, Yue Gao, Delphine Jenny Vandeputte, Martine Leermakers, Joske Ruytinx

{"title":"Arbuscular Mycorrhizal Fungi Improve Rice Production in Zinc-Amended Soils by Altering Zinc Transport and Translocation Routes.","authors":"Yang Zhang, Yue Gao, Delphine Jenny Vandeputte, Martine Leermakers, Joske Ruytinx","doi":"10.1111/pce.15543","DOIUrl":"https://doi.org/10.1111/pce.15543","url":null,"abstract":"Human activities including industry and overcultivation resulted in marginal soils, unbalanced in nutrients or polluted with heavy metals. Zinc (Zn) is an essential micronutrient and its nonoptimal soil bio-availability, negatively affects plant growth and production. Arbuscular mycorrhizal fungi (AMF) could improve Zn acquisition in limited conditions and prevent accumulation in plant tissue in contaminated soils. However, it is not clear how AMF impact host plant Zn uptake and transport routes. In this study we assessed the potential of commercial AMF inoculum to support rice growth and production in non-Zn-fertilised, Zn-fertilised and Zn-polluted soils alongside their impact on host plant nutrient balances and Zn uptake and translocation routes. The results demonstrated that AMF inoculation restores rice growth and grain production in Zn-amended soils and that Zn amendment improves root colonisation. Shoot ionomes were particularly sensitive to differences in Zn supply and differentially affected in AMF and mock-inoculated plants. When present in excess, AMF inoculation decreased accumulation of Zn in shoots and disturbed Zn-P (phosphorus) relationship. We could not detect a mycorrhiza-specific Zn transporter in rice but rather a modification of expression for Zn transporters in the direct uptake routes. AMF inoculation interacts with the Zn-dependent response of heavy metal ATPase (OsHMA) transporters involved in root-to-shoot translocation. All together, these data indicate a change in relative importance of different direct Zn transport routes upon AMF colonisation. These findings provide valuable insights into how AMF symbiosis influences Zn uptake and distribution in rice under varying Zn conditions, allowing for the development of plant-fungus bioremediation and biofortification technologies.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The GhWRKY46-GhGAI Module Mediates Cotton Flowering by Regulating the Expression of Flowering Promotion Factors. GhWRKY46-GhGAI模块通过调控棉花开花促进因子的表达介导棉花开花。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-04-08 DOI: 10.1111/pce.15533

Mengxi Sun, Miaoqian Yang, Xinyu Dang, Xiaokang Fu, Jianhua Lu, Liang Ma, Shuxun Yu, Hengling Wei, Hantao Wang

{"title":"The GhWRKY46-GhGAI Module Mediates Cotton Flowering by Regulating the Expression of Flowering Promotion Factors.","authors":"Mengxi Sun, Miaoqian Yang, Xinyu Dang, Xiaokang Fu, Jianhua Lu, Liang Ma, Shuxun Yu, Hengling Wei, Hantao Wang","doi":"10.1111/pce.15533","DOIUrl":"https://doi.org/10.1111/pce.15533","url":null,"abstract":"Flowering represents a pivotal developmental transition stage in the life cycle of a plant, and the occurrence of flowering at the optimal time is critical for reproductive success. WRKY transcription factors play a vital role in a signaling network that governs a multitude of plant processes. Here, a gene, GhWRKY46, that was differentially expressed in early and late maturing materials was identified via association analysis, and it was specifically expressed in flower buds. Under natural light and temperature conditions, compared to Jin668, the flowering time of OE-GhWRKY46 plants was advanced by approximately 6 days, while the flowering time of CR-GhWRKY46 plants was delayed by approximately 8 days. Transcriptomic data indicated that overexpression or knockout of GhWRKY46 resulted in the activation or repression, respectively, of the photoperiod gene CO-Like and genes related to bud differentiation. Combined RNA-seq and DAP-seq analysis revealed that three genes, namely, GhCOL4, GhCOL2 and GhFPF1-like, may be expressed downstream of GhWRKY46. Dual-luciferase assays and electrophoretic mobility shift assays (EMSAs) demonstrated that GhWRKY46 could directly bind to the W-box and promote the expression of these genes. Similarly, GhFT was also found to be activated by GhWRKY46. Both in vivo and in vitro biochemical analyses demonstrated that GhWRKY46 interacted with GhGAI, and GhGAI could interfere with the transcriptional activation of GhWRKY46, which in turn inhibited the expression of GhCOL4, GhCOL2, GhFPF1-like, and GhFT. In conclusion, this study accurately predicted the GhWRKY46 binding motif, which is important for the construction of regulatory networks of the WRKY family in other crops and introduces a novel regulatory module for the flowering regulation pathway in cotton.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simplified and Integrated View of Disease Control in Varietal Mixtures Using the Phytobiome Framework. 利用植物群系框架简化和综合看待品种混合病害控制。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-04-08 DOI: 10.1111/pce.15535

Laura Mathieu, Elsa Ballini, Jean-Benoit Morel

{"title":"A Simplified and Integrated View of Disease Control in Varietal Mixtures Using the Phytobiome Framework.","authors":"Laura Mathieu, Elsa Ballini, Jean-Benoit Morel","doi":"10.1111/pce.15535","DOIUrl":"https://doi.org/10.1111/pce.15535","url":null,"abstract":"Increasing intraspecific diversity within crop systems is a promising strategy to manage aerial diseases, particularly those caused by fungal aerial pathogens. This review examines how cultivar mixtures reduce disease incidence and severity using the phytobiome framework, identifying three major types of processes: (1) physical ones, which alter disease dynamics through dilution effects, barrier effects, and microclimate modifications; (2) processes that are mediated by microbial interactions, which influence disease severity via induced resistance and indirect plant-plant interactions mediated by the microbiome; and (3) processes involving direct plant-plant interactions, where danger signaling and signaling from healthy neighbors modulate plant physiology and immunity through resource management and molecular cues. This review provides a comprehensive understanding of how cultivar mixtures enhance disease resistance and emphasizes that direct plant-plant interactions are likely stronger contributors than so far considered. It highlights the need for further research into the roles of microbiomes and direct plant-plant interactions to optimize mixtures' performance.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shade-Induced Regulation of Cell Wall Thickening in Moso Bamboo: A Molecular Pathway Involving XTH Activity. 遮荫诱导毛竹细胞壁增厚的调控：一个涉及XTH活性的分子途径。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-04-08 DOI: 10.1111/pce.15530

Yali Xie, Junlei Xu, Xiangyi Li, Yuping Dou, Ruiman Geng, Zhanchao Cheng, Chongyang Wu, Jian Gao

{"title":"Shade-Induced Regulation of Cell Wall Thickening in Moso Bamboo: A Molecular Pathway Involving XTH Activity.","authors":"Yali Xie, Junlei Xu, Xiangyi Li, Yuping Dou, Ruiman Geng, Zhanchao Cheng, Chongyang Wu, Jian Gao","doi":"10.1111/pce.15530","DOIUrl":"https://doi.org/10.1111/pce.15530","url":null,"abstract":"How plants adapt to shade and perform specific morphogenesis is one of the secrets of the kingdom, and unravelling the underlying molecular mechanisms is crucial. Scanning electron microscope results have revealed that a low R/FR ratio (indicating shade conditions) inhibited the cell wall thickness of parenchyma cells and sieve tube cells of Moso bamboo (Phyllostachys edulis). Xyloglucan Endotransglucosylase/hydrolase3 (PheXTH3) was identified as a circadian rhythm gene responsive to a low R/FR ratio by qRT-PCR analysis, and it showed peak activity in the vascular system. PheXTH3 enhanced interfascicular fibre cell wall thickening and lignin accumulation in stable transgenic Arabidopsis thaliana lines. A low R/FR ratio downregulated PheXTH3 expression, subsequently suppressing cell wall thickening in vessel and interfascicular fibre cells. Both Yeast One-Hybrid experiments and Dual-LUC assays revealed that WOX3b1, AP2-39, and XTH3 may form a regulatory pathway. Collectively, we proposed a WOX3b1-AP2-39-XTH3 molecular pathway mediated by the ratio of low R/FR, which may regulate the thickening of cell wall in Moso bamboo.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Pepper E3 Ligase CaGIR1 Acts as a Negative Regulator of Drought Response via Controlling CaGRAS1 Stability. 辣椒E3连接酶CaGIR1通过控制CaGIR1稳定性作为干旱响应的负调控因子

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-04-08 DOI: 10.1111/pce.15516

Woonhee Baek, Donghyuk Oh, Lim Chae Woo, Sung Chul Lee

{"title":"The Pepper E3 Ligase CaGIR1 Acts as a Negative Regulator of Drought Response via Controlling CaGRAS1 Stability.","authors":"Woonhee Baek, Donghyuk Oh, Lim Chae Woo, Sung Chul Lee","doi":"10.1111/pce.15516","DOIUrl":"https://doi.org/10.1111/pce.15516","url":null,"abstract":"The ubiquitin-proteasome pathway modulates protein stability, which impacts plant responses to abiotic stresses, such as drought. Our previous study reported that the pepper GRAS-type transcription factor CaGRAS1 plays a positive role in drought resistance. However, the mechanism by which drought stress affects CaGRAS1 protein stability remains unknown. Here, we identified Capsicum annuum CaGRAS1-Interacting RING-type E3 ligase 1 (CaGIR1) through yeast two-hybrid analysis. The interaction between these two proteins was confirmed by both in vitro and in vivo assays, and interaction occurred in both the nucleus and cytoplasm, consistent with their subcellular localisation. In ubiquitination assays, CaGIR1 was shown to have ubiquitin E3 ligase activity, which is dependent on its RING domain. CaGIR1 also directly ubiquitinated CaGRAS1 in vitro and in vivo, and CaGRAS1 protein stability negatively correlated with CaGIR1 expression levels. In contrast to CaGRAS1, CaGIR1 was found to play a negative role in drought resistance. Phenotypic assays revealed that the silencing of CaGIR1 in pepper resulted in enhanced drought resistance through the modulation of stomatal responses and drought-responsive marker gene expression, whereas CaGIR1 overexpression led to the opposite results in Arabidopsis. Overall, our findings suggest that CaGIR1 negatively modulates ABA and drought responses by triggering CaGRAS1 protein degradation.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unsaturation of Leaf Air Spaces Sheds New Light on the Role of Aquaporins. 叶片空气空间的不饱和为水通道蛋白的作用提供了新的思路。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-04-08 DOI: 10.1111/pce.15548

Haoyu Diao, Marco M Lehmann, Arthur Gessler

引用次数: 0

Arbuscular Mycorrhizal Fungi Promote Nodulation and N₂ Fixation in Soybean by Specific Root Exudates. 丛枝菌根真菌通过特定根系分泌物促进大豆结瘤和固氮。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-04-08 DOI: 10.1111/pce.15529

Yuanyuan Li, Luwen Lu, Qianqian Wang, Xiangdong Liu, Jiang Tian, Ruifu Zhang, Hong Liao, Hans Lambers, Xiurong Wang

{"title":"Arbuscular Mycorrhizal Fungi Promote Nodulation and N2 Fixation in Soybean by Specific Root Exudates.","authors":"Yuanyuan Li, Luwen Lu, Qianqian Wang, Xiangdong Liu, Jiang Tian, Ruifu Zhang, Hong Liao, Hans Lambers, Xiurong Wang","doi":"10.1111/pce.15529","DOIUrl":"https://doi.org/10.1111/pce.15529","url":null,"abstract":"Legume plants commonly associate with both arbuscular mycorrhizal (AM) fungi and rhizobia and thus enhance the acquisition of phosphorus (P) and nitrogen (N) nutrition. Inoculation with AM fungi can promote nodulation and N2 fixation of legume plants; however, the underlying mechanisms remain poorly understood. Here, root exudates collected from AM-colonised soybean plants showed greater accumulation of the specific flavonoids (daidzein and genistein) and phenolic acids (benzoic acid and p-Hydroxybenzoic acid), and significantly promoted nodulation. Furthermore, the exudates from AM-colonised roots and the derived specific flavonoids and phenolic acids effectively increased rhizobial growth, chemotaxis, biofilm formation. Addition of the specific synthetic root exudates enhanced nodulation and N2 fixation, and expression of the core nodulation genes in soybean. Overexpression of a phenylalanine ammonia-lyase gene, GmPAL2.4 markedly upregulated the expression of the genes related to the biosynthesis of daidzein, genistein, benzoic acid, and p-Hydroxybenzoic acid, and increased accumulation of these specific flavonoids and phenolic acids in the transgenic plants, thus enhancing nodulation and N2 fixation. In summary, we demonstrated a crucial role of specific flavonoids and phenolic acids induced by AM symbiosis in promoting rhizobium-host symbiosis. This offers a pathway for improving symbiotic efficiency through the use of specific synthetic compounds.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Importin-β Protein LbSAD2 Enhances Salt Gland Development and Salt Resistance in the Recretohalophyte Limonium bicolor. 输入蛋白β LbSAD2促进双色盐生植物盐腺发育和耐盐性。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-04-08 DOI: 10.1111/pce.15524

Qiuyu Ma, Boqing Zhao, Xiangmei Jiao, Yaqiong Sui, Mingfa Sun, Jianfei Ma, Jingwen Zhu, Baoshan Wang, Fang Yuan

{"title":"The Importin-β Protein LbSAD2 Enhances Salt Gland Development and Salt Resistance in the Recretohalophyte Limonium bicolor.","authors":"Qiuyu Ma, Boqing Zhao, Xiangmei Jiao, Yaqiong Sui, Mingfa Sun, Jianfei Ma, Jingwen Zhu, Baoshan Wang, Fang Yuan","doi":"10.1111/pce.15524","DOIUrl":"https://doi.org/10.1111/pce.15524","url":null,"abstract":"Limonium bicolor is a typical recretohalophyte with specialised salt glands that secrete excessive Na+ out of the plant. The detailed mechanisms of salt gland development and salt resistance are largely unclear. Here, we investigated the function of the importin-β protein LbSAD2 from L. bicolor. Lines with silenced LbSAD2 expression showed significantly lower salt gland density, salt-secretion ability and salt resistance, whereas LbSAD2 overexpression lines had a greater number of salt glands with an abnormal distribution in the abaxial and leaf adaxial surfaces. A previously uncharacterised hydrophobic protein, Lb2G12077, can bind to the LbSAD2 promoter to inhibit the transcription of LbSAD2 verified by a yeast one-hybrid, electrophoretic mobility shift and dual-luciferase reporter assay. Further functional validation revealed that Lb2G12077 demoted salt gland development and salt resistance of L. bicolor. Moreover, a yeast two-hybrid, bimolecular fluorescence complementation and GST pull-down assays indicated that the hypothetical protein Lb2G12567 interacts with LbSAD2, whose silenced lines also showed significantly reduced salt gland density, salt-secretion ability and salt resistance, suggesting Lb2G12567 played a similar positive role in salt resistance. The current LbSAD2 pathway sheds light on salt gland development and salt resistance in L. bicolor, laying the foundation for increasing salt tolerance in crops.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VaEIN3.1-VaERF057-VaFBA1 Module Positively Regulates Cold Tolerance by Accumulating Soluble Sugar in Grapevine. VaEIN3.1-VaERF057-VaFBA1模块通过积累葡萄可溶性糖正向调节葡萄抗寒性。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-04-08 DOI: 10.1111/pce.15522

Huimin Zhou, Yujun Hou, Lisha Tan, Qingyun Li, Wenjuan Li, Subash Kafle, Meilong Xu, Konstantin V Kiselev, Lin Meng, Haiping Xin

{"title":"VaEIN3.1-VaERF057-VaFBA1 Module Positively Regulates Cold Tolerance by Accumulating Soluble Sugar in Grapevine.","authors":"Huimin Zhou, Yujun Hou, Lisha Tan, Qingyun Li, Wenjuan Li, Subash Kafle, Meilong Xu, Konstantin V Kiselev, Lin Meng, Haiping Xin","doi":"10.1111/pce.15522","DOIUrl":"https://doi.org/10.1111/pce.15522","url":null,"abstract":"Ethylene-responsive transcription factors (ERFs) were widely found to participate in cold response in plants. But the underlying regulatory mechanism of each cold-induced ERFs remains to be elucidated. Previously, we identified VaERF057 as a cold-induced gene in Vitis amurensis, a cold-hardy wild Vitis species. Here we found that overexpression of VaERF057 (VaERF057-OE) enhanced the freezing tolerance of V. amurensis roots. While VaERF057 knockdown tissues show decreased cold tolerance than control. DAP-seq combined with transcriptome data (VaERF057-OE roots) allowed to identify VaFBA1 (fructose-1,6-bisphosphate aldolase) as a downstream target of VaERF057. VaERF057 can bind to the VaFBA1 promoters and activate its expression. VaERF057-OE roots show increased expression of VaFBA1 and high content of soluble sugar than the control, whereas VaERF057 knockdown tissues showed opposite changes. Results from OE and knockdown material also support the role of VaFBA1 in regulating soluble sugar content and cold tolerance in grapevines. Furthermore, cold-induced expression of VaERF057 was found to be regulated by ethylene-insensitive3-1 (VaEIN3.1). Overexpression of VaEIN3.1 enhanced the transcription of VaERF057 and VaFBA1, the content of soluble sugar and cold tolerance in grapevine. VaEIN3.1 knockdown tissues show opposite trends when compared to VaEIN3.1-OE lines. Together, these results suggested a positive contribution of VaEIN3.1-VaERF057-VaFBA1 module in response to cold stress in grapevine.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The m⁶A Reader MhYTP2 Regulates MdERF54 mRNA Stability and Contributes to Hypoxia Tolerance in Apple (Malus domestica). m6A读写器MhYTP2调控苹果MdERF54 mRNA稳定性并参与耐缺氧

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-04-07 DOI: 10.1111/pce.15527

Tianli Guo, Zehua Yang, Ru Bao, Xiao Li, Na Wang, Chao Li, Ke Mao, Xiaoqing Gong, Changhai Liu, Fengwang Ma

{"title":"The m6A Reader MhYTP2 Regulates MdERF54 mRNA Stability and Contributes to Hypoxia Tolerance in Apple (Malus domestica).","authors":"Tianli Guo, Zehua Yang, Ru Bao, Xiao Li, Na Wang, Chao Li, Ke Mao, Xiaoqing Gong, Changhai Liu, Fengwang Ma","doi":"10.1111/pce.15527","DOIUrl":"https://doi.org/10.1111/pce.15527","url":null,"abstract":"Hypoxia is one of the main challenges in apple (Malus domestica) cultivation. However, breeding hypoxia-tolerant cultivars demands a thorough understanding of the responses of apple trees to low oxygen supply. Studies have indicated that N6-methyladenosine (m6A) reader regulates plant stress response by binding to their corresponding mRNA targets with m6A modification. The present study investigated the function and mechanism of apple m6A reader MhYTP2 under hypoxia stress. Here, we found that the overexpression of MhYTP2 improved hypoxia resistance in apple. Previous RNA immunoprecipitation sequencing (RIP-seq) results identified the mRNA of Ethylene Response Factor 54 (ERF54) as a direct target of MhYTP2; electronic mobility shift assays (EMSA) further verified this finding. Further transcription inhibition assays demonstrated that MhYTP2 increased MdERF54 mRNA stability. Under hypoxia stress, MdERF54 increased the activities of pyruvate decarboxylase (PDC), lactate dehydrogenase (LDH), and alcohol dehydrogenase (ADH) the key enzymes in anaerobic respiration pathway, activated the ethylene signalling pathway, increased the chlorophyll content of plant leaves and photosynthetic rates, enhanced the adaptability of roots, reduced the damage to biofilm and antioxidant system, and enhanced the antioxidant capacity. Thus, our results elucidated the molecular mechanisms by which the MhYTP2-MdERF54 module influences the response of the apple to hypoxia stress.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0