Plant, Cell & Environment最新文献

{"title":"Continuous In-Situ Water Stable Isotopes Reveal Rapid Changes in Root Water Uptake by Fagus sylvatica During Severe Drought.","authors":"Laura Kinzinger, Simon Haberstroh, Judith Mach, Markus Weiler, Natalie Orlowski, Christiane Werner","doi":"10.1111/pce.70055","DOIUrl":"https://doi.org/10.1111/pce.70055","url":null,"abstract":"<p><p>Adaptation of root water uptake (RWU) is critical for drought resilience in temperate forest trees, yet information on water sources and uptake depths dynamics is scarce. Continuous in-situ stable isotope measurements in soil and xylem water of Fagus sylvatica during the severe drought 2022 revealed daily changes in RWU depth and water ages. Xylem water comprised mainly recent precipitation in early summer, but winter and spring precipitation contributed up to 70% during drought, with longer transit times (206 ± 60 days) compared to summer precipitation (62 ± 11 days). Concurrently, trees shifted RWU to deeper soil layers while also responding to individual precipitation events by absorbing fresh precipitation from topsoil layers within 2-4 days, demonstrating the significance of individual precipitation events for tree water dynamic. F. sylvatica used > 80% of a fresh precipitation event before drought, but < 20% during recovery, indicating potential drought legacies on precipitation use. Unravelling these rapid dynamics in RWU and water ages offers novel insights into the importance of single and seasonal precipitation events for forest water fluxes.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional Dynamics of Rice Under Individual, Combined, and Sequential Abiotic Stresses: Insights Across Stress Severity and Recovery in Contrasting Genotypes.","authors":"Khalid Anwar, Shivani Kansal, Rajeev Nayan Bahuguna, Saurabh Raghuvanshi, Sneh L Singla-Pareek, Rita A Sharma, Ashwani Pareek","doi":"10.1111/pce.70062","DOIUrl":"https://doi.org/10.1111/pce.70062","url":null,"abstract":"<p><p>Plants in natural environments frequently encounter multiple abiotic stresses, which may occur individually, simultaneously, or sequentially, significantly impacting crop productivity. The transcriptional response to these stresses varies across genotypes, and understanding these variations at the molecular level is critical for improving stress resilience. In a previous study, we identified two contrasting rice genotypes, Lomello (highly stress-tolerant) and C57-5043 (highly stress-sensitive), from a screen of ~400 genotypes for abiotic stress tolerance. Here, we performed time-course transcriptomic profiling to dissect the molecular basis of their differential stress responses under varying severity levels (mild, moderate, and severe) of high temperature (HT), drought (D), submergence (S), combined heat and drought stress (HTD), and post-submergence drought (PSD). Our results reveal that transcriptional responses are highly context-dependent, with distinct expression patterns emerging under individual, combined, and sequential stress conditions. Notably, while submergence induced significant transcriptomic changes within the first few days, subsequent drought exposure, particularly at higher severity levels, did not elicit a strong transcriptional response, likely due to transcriptional silencing resulting from physiological damage. Comparative analysis between genotypes showed that Lomello exhibits constitutively higher expression of genes involved in phytoalexin biosynthesis, even in the absence of stress, potentially conferring a preemptive defense advantage. Furthermore, Lomello demonstrated a robust induction of genes associated with reactive oxygen species (ROS) scavenging, abscisic acid (ABA) biosynthesis and signaling, and secondary metabolite production in response to stress, followed by a rapid reversion to near-baseline expression levels during recovery. These findings suggest that Lomello's superior stress tolerance is driven by enhanced secondary metabolite accumulation, efficient ROS detoxification, and a stronger recovery response. This study provides novel insights into genotype-specific transcriptional strategies for stress resilience, offering potential targets for breeding climate-resilient rice varieties.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Identification and Characterisation of Aquaporins in the Marine Higher Plant Zostera japonica and Their Response to the Intertidal Environment.","authors":"Hongzhen Wang, Yamei Wang, Feng He, Xinqi Li, Yu Zang, Song Xue, Jiayi Xin, Xuexi Tang, Jun Chen","doi":"10.1111/pce.70056","DOIUrl":"https://doi.org/10.1111/pce.70056","url":null,"abstract":"<p><p>Zostera japonica mainly lives in the intertidal zone and is susceptible to fluctuations in abiotic stresses, making it an excellent natural model for studying plant response mechanisms in intertidal environments. Aquaporins (AQPs) play important roles in water uptake, growth and development, and stress regulation. However, studies on the functions of the AQPs of Z. japonica (ZjAQPs) in response to intertidal environments have not been reported. In this study, we identified 22 AQPs in Z. japonica. Phylogenetic analysis revealed that ZjAQPs can be divided into four subfamilies: PIP, TIP, NIP and SIP. Analysis of the cis-acting elements suggested that ZjAQPs may be responsive to stresses. The subcellular localisation of ZjAQPs in tobacco revealed that they functioned predominantly in the membrane system. Transcript expression revealed that ZjAQPs were specifically expressed in various tissues and presented complex responses to environmental variables. Furthermore, transgenic Overexpression in yeast and Arabidopsis revealed that ZjNIP2-1, ZjSIP2-3, ZjPIP1-3, and ZjTIP1-3 play pivotal roles in adaptation to drought and salinity stress. Unlike the AQP subfamily of other higher plants, NIPs and SIPs may play important roles in Z. japonica. The information provided here will help to understand the precise role of AQP in Z. japonica adaptation to intertidal environments.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordination of Phytomelatonin and Salicylic Acid Signalling in Stomatal Closure.","authors":"Xue Li, Lin Xiao, Yanjie Song, Xingyang Wei, Zed Rengel, Yanli Chen, Kunzhi Li, Qi Chen","doi":"10.1111/pce.70048","DOIUrl":"https://doi.org/10.1111/pce.70048","url":null,"abstract":"<p><p>Phytomelatonin (PMT) and salicylic acid (SA) play crucial roles in stomatal closure and immune regulation, yet their regulatory mechanisms are not fully understood. Here, we found that SA-induced stomatal closure and ROS production are dependent on the phytomelatonin receptor 1 (PMTR1) via regulating the expression of genes associated with cell wall peroxidases PRX33 and PRX34. Exogenous melatonin (exMT) induces the expression of genes related to SA biosynthesis (ICS1, PBS3), the receptor NPR1, as well as the effector transcription factors TGA1-3. Furthermore, exMT promotes the nuclear localisation of NPR1 through NO and GSH signalling in a PMTR1-dependent manner. Mutants of npr1, as well as tga1, tga2 and tga3, show partial sensitivity to PMTR1-mediated exMT signalling in stomatal closure. Either exMT or SA can significantly increase PMTR1 expression in Col-0, but not in npr1-1 and tga1 mutant plants. TGA1 can directly bind to the promoter elements of PMTR1 and activate its expression. Furthermore, the exMT- and SA-induced stomatal closure plays a vital role in preventing bacterial invasion in a PMTR1-dependent manner. Collectively, the results presented here demonstrate the coordination of PMTR1 and NPR1 in the regulation of PMT and SA signals in stomatal closure and immunity.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standing on the Shoulder of Giants: Developmental Regulators (DRs) Are Past Gifts for Future Plant Engineering.","authors":"Gaojie Li, Hongwei Hou","doi":"10.1111/pce.70072","DOIUrl":"https://doi.org/10.1111/pce.70072","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding the Multifunctionality of B-Box Proteins: Bridging Light, Stress and Developmental Networks in Diverse Plant Species.","authors":"Jiaxin Li, Chang Liu, Zhen Zhang, Ningbo Zhang, Weirong Xu","doi":"10.1111/pce.70061","DOIUrl":"https://doi.org/10.1111/pce.70061","url":null,"abstract":"<p><p>B-box (BBX) transcription factors are emerging as pivotal regulators of environmental adaptation and developmental plasticity in plants. These proteins act at the intersection of light, hormonal and stress signalling networks to modulate key processes, including photomorphogenesis, circadian rhythm regulation, abiotic and biotic stress responses, anthocyanin biosynthesis and flowering time control. Recent studies in various model species and crops have revealed that BBX proteins can function as both activators and repressors of transcription, often by directly interacting with key regulators such as HY5, PRR9/7 and MYC2. These interactions enable BBX factors to fine-tune gene expression in response to dynamic environmental conditions. Functionally, BBX proteins orchestrate light-responsive development, enhance tolerance to drought, salinity, and pathogens via hormonal and reactive oxygen species (ROS)-mediated pathways, and regulate secondary metabolism linked to pigment accumulation. Their roles in reproductive development, particularly in controlling flowering time and vegetative-reproductive phase transitions, position them as promising targets for crop improvement. Despite growing insight, key knowledge gaps remain. The mechanistic basis of BBX duality, their post-translational regulation and their integration within broader transcriptional and chromatin networks are still poorly understood. Additionally, BBX-mediated signalling remains understudied in monocots, wild relatives and under complex field conditions. This review summarizes the latest mechanistic and evolutionary insights into BBX transcription factors, emphasizing their functional diversity, context-dependent regulation, and applications in precision breeding. By highlighting both translational applications and unresolved challenges, we propose future directions for using BBX proteins to design of climate-resilient, high-performance crops.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Redox and Hormone Signalling: A Central Role for Hydrogen Sulfide in Watermelon Immunity Against FON2.","authors":"Jing Zhang, Yanjie Xie","doi":"10.1111/pce.70059","DOIUrl":"https://doi.org/10.1111/pce.70059","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Verticillium dahliae Elicitor VdSP8 Enhances Disease Resistance Through Increasing Lignin Biosynthesis in Cotton\".","authors":"","doi":"10.1111/pce.70068","DOIUrl":"https://doi.org/10.1111/pce.70068","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-Invasive Phenotyping of Sugar Beet and Maize Roots Using Field-Scale Spectral Electrical Impedance Tomography.","authors":"Valentin Michels, Maximilian Weigand, Lena Lärm, Onno Muller, Andreas Kemna","doi":"10.1111/pce.70049","DOIUrl":"https://doi.org/10.1111/pce.70049","url":null,"abstract":"<p><p>Root systems are essential for plant water and nutrient uptake, but are difficult to characterize in-situ due to their inaccessibility. Spectral electrical impedance tomography (sEIT) is a non-invasive geoelectrical method that has shown potential to quantify root traits at the laboratory scale. However, field applications remain scarce due to technical limitations and challenges in separating soil and root polarization signatures. This study explores the use of sEIT for in-situ phenotyping of sugar beet and maize root systems. We conducted multi-frequency sEIT measurements at varying crop growth stages to derive the subsurface complex resistivity distribution. Spectral analysis revealed high-frequency polarization peaks for both species. Additionally, sugar beets exhibited an additional low-frequency peak late-season, which we attribute to the development of large storage parenchyma. For sugar beet, the high root-to-soil volume fraction allowed a direct correlation of electrical parameters to root biomass density. For maize, the superimposed soil polarization necessitated an alternative approach: We introduce an electrical root index ( <math> <semantics> <mrow><mrow><mi>ERI</mi></mrow> </mrow> <annotation>${ERI}$</annotation></semantics> </math> ) as a spectral dispersion measure indicative of root presence and show its correlation to root biomass density. Our findings demonstrate that sEIT is sensitive to macro- and microscopic root traits under field conditions, holding great potential for non-invasive phenotyping of plant roots.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wheat miRNA TaMIR5062-5A Targets Calmodulin TaCML31 That Cooperates With MYB Member TaMYB77 to Modulate Drought and Salt Responses.","authors":"Xiaoyang Hou, Chengjin Guo, Yuanjinzhi Qiao, Jiale Wang, Ziyi Wang, Jiaqi Zhang, Xinxin Shi, Xiaoxin Fu, Hongning Zhang, Kai Xiao","doi":"10.1111/pce.70038","DOIUrl":"https://doi.org/10.1111/pce.70038","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) are key regulators of plant growth, development, and abiotic stress tolerance, acting via posttranscriptional control of target genes. In this study, we identified and characterized TaMIR5062-5A, a miRNA member in Triticum aestivum, focusing on its role in mediating drought and salt stress responses. TaMIR5062-5A showed downregulated expression upon exposure to drought and salt stress conditions, whereas its target gene TaCML31, a calmodulin-encoding gene verified by dual luciferase (LUC) assay, displayed the opposite expression trend under the above stress conditions. These findings suggested its involvement in osmotic stress responses through the Ca²⁺-dependent signaling pathway. Protein interaction assays (i.e., yeast two-hybrid, bimolecular fluorescence complementation, and co-immunoprecipitation) revealed that TaCML31 could interact with the MYB transcription factor TaMYB77, indicating that TaMIR5062-5A, its target gene TaCML31, and TaMYB77 constitute a regulatory module in plant osmotic stress response. Transgene analysis confirmed that TaMIR5062-5A negatively while TaCML31 and TaMYB77 positively regulated drought and salt stress tolerance by regulating osmolyte accumulation, stomata closure, root formation, and reactive oxygen species (ROS) homeostasis. Yeast one-hybrid, transcriptional activation, and chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-PCR) assays indicated that TaMYB77 could bind to the promoters of osmotic stress-defense genes, namely TaP5CS2, TaNCED1, and TaDREB3, and regulate their transcription. The transgenic lines with knockdown expression of these stress response-associated genes exhibited impaired plant growth, reduced proline accumulation, dysregulated photosynthetic function, and compromised ROS homeostasis under drought and salt stress conditions, suggesting their roles in regulating plant osmotic stress responses underlying the miRNA module regulation. Strong correlations were observed between yield and the transcript levels of TaMIR5062-5A, TaCML31, and TaMYB77 in a wheat variety panel cultured under field drought conditions. Moreover, haplotype characterization of TaMIR5062-5A indicated that TaMIR5062-5A-Hap1 conferred enhanced drought tolerance in wheat plants. Overall, our findings establish a miRNA regulatory module, namely TaMIR5062-5A-TaCML31-TaMYB77, that plays an essential role in plant osmotic stress response in T. aestivum.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}