Plant, Cell & Environment最新文献_第10页

Optimised Ribosome Profiling Reveals New Insights Into Translational Regulation in Synchronised Chlamydomonas reinhardtii Cultures. 优化核糖体分析揭示了同步莱茵衣藻培养中翻译调控的新见解。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-06-11 DOI: 10.1111/pce.15681

Yen-Ling Lin, Eva Yuhua Kuo, Shih-Yi Wang, Chih-Chi Lee, Su-Chiung Fang

{"title":"Optimised Ribosome Profiling Reveals New Insights Into Translational Regulation in Synchronised Chlamydomonas reinhardtii Cultures.","authors":"Yen-Ling Lin, Eva Yuhua Kuo, Shih-Yi Wang, Chih-Chi Lee, Su-Chiung Fang","doi":"10.1111/pce.15681","DOIUrl":"https://doi.org/10.1111/pce.15681","url":null,"abstract":"Deep sequencing of ribosome footprints, also known as ribosome profiling (Ribo-seq), enables the quantification of mRNA translation and a comprehensive view of the translatome landscape. Here, we report an optimised Ribo-seq protocol and analysis pipeline for the model green alga, Chlamydomonas reinhardtiii (Chlamydomonas). Compared to the previously published data sets, the ribosome-protected fragments generated by our protocol showed improved mapping rates to the main open reading frames, reduced bias mapping to the gene coding regions and high 3-nt footprint periodicity. Using this optimised protocol, we employed Ribo-seq alongside RNA-seq to compute translation efficiency and identify genes with differential translation during the diurnal cycle. Interestingly, we found that the translation efficiency of many core cell cycle genes was significantly enhanced in cells at the early synthesis/mitosis (S/M) stage. This result suggests that translational regulation plays a role in cell cycle regulation in C. reinhardtii. Furthermore, the high periodicity of ribosome footprints allowed us to identify potential C. reinhardtii upstream open reading frames (uORFs). Further analysis revealed that some of these uORFs are differentially regulated and may play a role in diurnal regulation. In summary, we used an optimised Ribo-seq protocol to generate a high-quality Ribo-seq data set that constitutes a valuable resource for Chlamydomonas genomics. The ribosome profile data is linked to the Chlamydomonas reference genome and accessible to the scientific community.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental Gradients Override Phylogenetic Effects in Plant Adaptation to High Elevation. 环境梯度对植物高海拔适应的影响大于系统发育效应。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-06-10 DOI: 10.1111/pce.70005

Chunhua Lv, Pubin Hong, Mingxin Liu, Xiancheng Lu, Yuhao Feng, Zhiyao Tang, Yanhong Tang, Yin Wang

引用次数: 0

Herbivore Elicitors in Rice Defense: New Insights for Sustainable Crop Protection. 水稻防御中的草食激发子：可持续作物保护的新见解。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-06-09 DOI: 10.1111/pce.70001

Chi-Chou Chiu, Choun-Sea Lin

引用次数: 0

MusaDREB1G-Like Protein Modulates Cold and Drought Tolerance in Musa x paradisica. musadreb1g样蛋白调控天堂芭蕉的耐旱性和耐寒性

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-06-09 DOI: 10.1111/pce.15679

Subham Bhakta, Sanjana Negi, Pooja Bhatt, Yogendra Singh Rajpurohit, Thumballi R Ganapathi, Sudhir Singh, Himanshu Tak, Anand Ballal

引用次数: 0

Analysis of Genomic-Transcriptomic Dynamics Delineates Key Molecular Signatures Modulating Seed Size and Weight in Lentil. 小扁豆种子大小和重量调控的基因组转录动力学分析。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-06-09 DOI: 10.1111/pce.15671

Asish Kumar Padhy, Sangeeta Singh, Kuldeep Tripathi, Swarup Kumar Parida, Sabhyata Bhatia

{"title":"Analysis of Genomic-Transcriptomic Dynamics Delineates Key Molecular Signatures Modulating Seed Size and Weight in Lentil.","authors":"Asish Kumar Padhy, Sangeeta Singh, Kuldeep Tripathi, Swarup Kumar Parida, Sabhyata Bhatia","doi":"10.1111/pce.15671","DOIUrl":"https://doi.org/10.1111/pce.15671","url":null,"abstract":"Delineating key genetic determinants associated with seed size/weight is crucial for increasing productivity. In this study, the advantages of an integrated approach combining QTL mapping, GWAS and transcriptomics to identify robust candidates governing seed size and weight were demonstrated in lentil, an important grain legume. QTL mapping identified three stable QTLs harbouring 5113 genes. GWAS identified 42 MTAs (5 consistent) containing 192 underlying genes. Comparative transcriptome analysis identified 1202 differentially expressed transcripts. Integrated analysis of the results obtained from QTL mapping and GWAS revealed nine SNPs located in the three robust QTLs harbouring 32 candidate genes. Upon integration with transcriptome data, only one (LcWDL1) was identified as the most promising candidate. LcWDL1 (a member of TPX2 family involved in microtubule organisation and cell expansion) and its predicted interacting partners that is, LcGLIPs are known to function as regulators of seed size. Candidate gene-based association analysis identified a SNP on second exon of LcWDL1 to be significantly associated with seed size and weight of lentil. The genomic loci/candidate gene identified in the study will serve to expedite the molecular breeding and gene editing programs for enhancing seed size and seed weight in lentils.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic Chromatin Accessibility Underlies Generation-Biased Gene Expression in the Brown Alga Saccharina japonica. 褐藻Saccharina japonica的动态染色质可及性是世代偏倚基因表达的基础。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-06-09 DOI: 10.1111/pce.70008

Xiaoqi Yang, Xiuliang Wang, Jianting Yao, Delin Duan

引用次数: 0

The Highly Conserved Cys95 Residue of Fructose-1,6-Bisphosphatase 1 Mediates the pH-Driven Structure and Activity of the Enzyme and Photosynthesis. 果糖-1,6-双磷酸酶1高度保守的Cys95残基介导酶的ph驱动结构和活性以及光合作用。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-06-08 DOI: 10.1111/pce.15667

Samuel Gámez-Arcas, Edurne Baroja-Fernández, Francisco José Muñoz, Antonio J Serrato, Mónica Balsera, Ángela María Sánchez-López, Abdellatif Bahaji, Jesús Leal-López, Rafael Jorge León Morcillo, Javier Pozueta-Romero

{"title":"The Highly Conserved Cys95 Residue of Fructose-1,6-Bisphosphatase 1 Mediates the pH-Driven Structure and Activity of the Enzyme and Photosynthesis.","authors":"Samuel Gámez-Arcas, Edurne Baroja-Fernández, Francisco José Muñoz, Antonio J Serrato, Mónica Balsera, Ángela María Sánchez-López, Abdellatif Bahaji, Jesús Leal-López, Rafael Jorge León Morcillo, Javier Pozueta-Romero","doi":"10.1111/pce.15667","DOIUrl":"https://doi.org/10.1111/pce.15667","url":null,"abstract":"In Arabidopsis, exposure to microbial volatile compounds promotes thiol reduction of the Cys95 residue of the photosynthetic enzyme fructose-1,6-bisphosphatase (cFBP1). Although highly conserved in plants, the Cys95 function still remains unknown. We characterised recombinant wild-type (WT) cFBP1 and a variant (C95S) in which the Cys95 residue was replaced by serine. Furthermore, we characterised cFBP1-lacking cfbp1 transgenic plants expressing WT or C95S cFBP1. Cys95 replacement by serine reduced cFBP1 activity and its Mg2+ binding affinity and cooperativity. Although it is widely assumed that active cFBP1 is strictly homotetrameric, WT and C95S cFBP1 were present as inactive tetramers at pH 7.0 and active dimers at pH 8.3. At pH 7.8, WT and C95S cFBP1 were predominantly present as dimers and tetramers, respectively. WT cFBP1 expression totally reverted to WT the reduced photosynthetic activity of cfbp1 plants grown in the absence or presence of microbial volatiles, but that of C95S cFBP1 only partially did it. Artificial intelligence-based AlphaFold protein structure analyses predicted that the replacement of Cys95 by serine promotes cFBP1 conformational changes. We conclude that (i) active cFBP1 is strictly dimeric at pH values occurring in illuminated chloroplasts and (ii) Cys95 is an important determinant of the stromal pH-driven structure and activity of cFBP1 and photosynthesis.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating Multi-Trait Selection and Environmental Stability for Soybean (Glycine max L. Merr.) Genotype Improvement in Indonesia. 大豆多性状综合选择与环境稳定性研究印度尼西亚的基因型改良。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-06-06 DOI: 10.1111/pce.15662

Fadila Ridara, Debby Ustari, Arif Affan Wicaksono, Azka Algina, Suseno Amien, Gatut Wahyu Anggoro Susanto, Sri Koerniati, Bernadetta Rina Hastilestari, Vergel Concibido, Agung Karuniawan

{"title":"Integrating Multi-Trait Selection and Environmental Stability for Soybean (Glycine max L. Merr.) Genotype Improvement in Indonesia.","authors":"Fadila Ridara, Debby Ustari, Arif Affan Wicaksono, Azka Algina, Suseno Amien, Gatut Wahyu Anggoro Susanto, Sri Koerniati, Bernadetta Rina Hastilestari, Vergel Concibido, Agung Karuniawan","doi":"10.1111/pce.15662","DOIUrl":"https://doi.org/10.1111/pce.15662","url":null,"abstract":"Soybean (Glycine max L. Merr.) is a critical crop for food security and economic development in tropical regions, particularly in Indonesia. Despite its importance, the productivity of soybean in these areas is hindered by challenging climatic conditions, leading to a reliance on imports from temperate regions. Here, we identify genotypes that exhibit superior agronomic traits and stability, thereby contributing to improved soybean productivity in tropical environments. The multi-trait genotype-ideotype distance index (MGIDI) and multi-trait stability index (MTSI) were employed to evaluate the genotypes' performance in relation to an ideal ideotype and their stability across the environments. Additionally, the Genotype Main Effect Plus G × E Interaction (GGE) Biplot analysis was utilised to examine genotype-by-environment interactions and to identify the most suitable environments for soybean cultivation. The MGIDI index analysis identified the 100-seed weight (W100) as a key trait with the highest selection gain. The MTSI identified genotypes G11, G18 and G14 as the most stable across all environments. The GGE Biplot analysis indicated E2 (Upland, rainy season) as the most ideal environment for selecting superior soybean genotypes, suggesting it is the optimal setting for future breeding efforts. This study highlighted the importance of multi-trait selection and stability analysis in identifying soybean genotypes with both desirable traits and environmental stability for diverse tropical conditions. These findings offer strategic insights for future soybean breeding and cultivation in tropical regions.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small G Protein OsRab6a Confers Cold Tolerance by Cooperating With OsRAN2 and Suppressing ABA Signalling in Rice. 小G蛋白OsRab6a通过与OsRAN2合作并抑制ABA信号传导而赋予水稻抗寒性

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-06-06 DOI: 10.1111/pce.15665

Chenggang Li, Rubin Chen, Fenglin Huang, Hongjun Xie, Yue Chen, Yinghui Xiao, Su Jiang, Huqiang He, Shipeng Huang, Guangzhao Li, Chuanxin Chen, Yuancheng Sun, Jun Wu, Dan Wang

{"title":"Small G Protein OsRab6a Confers Cold Tolerance by Cooperating With OsRAN2 and Suppressing ABA Signalling in Rice.","authors":"Chenggang Li, Rubin Chen, Fenglin Huang, Hongjun Xie, Yue Chen, Yinghui Xiao, Su Jiang, Huqiang He, Shipeng Huang, Guangzhao Li, Chuanxin Chen, Yuancheng Sun, Jun Wu, Dan Wang","doi":"10.1111/pce.15665","DOIUrl":"https://doi.org/10.1111/pce.15665","url":null,"abstract":"Global climate change has increased the frequency of extreme low-temperature events, severely affecting rice production worldwide. Although numerous cold-tolerance genes have been mapped and cloned over the past four decades, the molecular mechanisms governing rice's response to cold stress remain incompletely understood. In this study, we employed an integrated approach combining reverse genetics, biochemical assays, and molecular biology to elucidate the role of OsRab6a, a small GTP-binding protein, in rice cold tolerance. Functional analyses demonstrated that OsRab6a overexpression enhances cold tolerance in transgenic rice, whereas OsRab6a mutants display heightened cold sensitivity at both the bud and seedling stages. Cold stress strongly induced OsRab6a expression, particularly in seedling roots. Mechanistically, OsRab6a physically interacts with OsRAN2, a known cold-resistance protein, under cold stress and acts upstream of OsRAN2 in the cold response pathway. Furthermore, OsRab6a upregulates OsPYL3 to modulate ABA signalling during cold stress. Our findings reveal that OsRab6a is a critical regulator of both cold tolerance and ABA signalling in rice. This study advances the understanding of OsRab6a-mediated cold stress responses and provides a valuable genetic engineering target for improving cold tolerance in rice.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Group 6 LEA Protein Plays Key Roles in Tolerance to Water Deficit, and in Maintaining the Glassy State and Longevity of Seeds. 6组LEA蛋白在种子耐水分亏缺、维持玻璃态和寿命中起关键作用。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-06-05 DOI: 10.1111/pce.15649

Inti A Arroyo-Mosso, H Nicholay Diaz-Ardila, Alejandro Garciarrubio, U G V S S Kumara, David F Rendón-Luna, Teresa B Nava-Ramírez, Thomas C Boothby, José Luis Reyes, Alejandra A Covarrubias

{"title":"A Group 6 LEA Protein Plays Key Roles in Tolerance to Water Deficit, and in Maintaining the Glassy State and Longevity of Seeds.","authors":"Inti A Arroyo-Mosso, H Nicholay Diaz-Ardila, Alejandro Garciarrubio, U G V S S Kumara, David F Rendón-Luna, Teresa B Nava-Ramírez, Thomas C Boothby, José Luis Reyes, Alejandra A Covarrubias","doi":"10.1111/pce.15649","DOIUrl":"https://doi.org/10.1111/pce.15649","url":null,"abstract":"Plants have a wide range of adaptive and protective mechanisms to cope with dehydration. Central in these processes are the Late Embryogenesis Abundant (LEA) proteins, whose levels notably increase in response to dehydration during seed development and vegetative tissues. Understanding the function of LEA proteins is essential for gaining insights into plant development and their adjusting responses to environmental stress. This study focuses on Group 6 LEA proteins (LEA6) from Arabidopsis thaliana: AtLEA6-2.1, AtLEA6-2.2, and AtLEA6-2.3. Phylogenetic analysis reveals that LEA6 family emerged with seed plants, pointing to a unique role in seed viability. Functional characterization using T-DNA insertion mutants demonstrated that AtLEA6-2.1, but not AtLEA6-2.2, is essential for tolerance to high-osmolarity and salinity during germination and post-germination growth. AtLEA6-2.1 deficiency also altered root architecture under salinity, increasing primary root length while reducing lateral root number and length, suggesting a role in root development not described before for a LEA protein. Furthermore, AtLEA6-2.1 is critical for seed longevity, as mutants lacking this protein showed reduced germination after natural and accelerated aging. These mutants exhibited increased glass-former fragility, indicating that AtLEA6-2.1 deficiency reduces cellular viscosity, which we found correlates with reduced longevity. Our investigation extends to protective protein assays under dehydration, revealing that the acidic nature of this protein family requires specific conditions for its In Vitro protective activity. Overall, this study underscores the essential role of AtLEA6-2.1 in the plant response to low-water availability, seed longevity, and glassy state properties, making it a potential target for enhancing plant resilience to environmental challenges.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0