Plant, Cell & Environment最新文献_第10页

GIGANTEA Is Required for Robust Circadian Rhythms in Wheat.

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-02-26 DOI: 10.1111/pce.15447

Laura J Taylor, Gareth Steed, Gabriela Pingarron-Cardenas, Lukas Wittern, Matthew A Hannah, Alex A R Webb

{"title":"GIGANTEA Is Required for Robust Circadian Rhythms in Wheat.","authors":"Laura J Taylor, Gareth Steed, Gabriela Pingarron-Cardenas, Lukas Wittern, Matthew A Hannah, Alex A R Webb","doi":"10.1111/pce.15447","DOIUrl":"https://doi.org/10.1111/pce.15447","url":null,"abstract":"GIGANTEA (GI) is a plant-specific protein that functions in many physiological processes and signalling networks. In Arabidopsis, GI has a central role in circadian oscillators regulating the abundance of ZEITLUPE and TIMING OF CAB EXPRESSION 1 proteins and is essential for photoperiodic regulation of flowering. We have investigated how ortholgues of this component of Arabidopsis circadian oscillators contribute to circadian rhythms and yield traits, including heading (flowering) in wheat. We find that GI is a core component of wheat circadian oscillators that is necessary to maintain robust oscillations in chlorophyll fluorescence and circadian oscillator transcript abundance. The predicted lack of functional GI results in later flowering of wheat in both long days and short days in controlled environment conditions. Our results support and extend previous work, which suggests that the pathways by which photoperiodism regulates flowering are not fully conserved between Arabidopsis and wheat. Understanding the molecular basis for photoperiodism in wheat is important for breeders looking to manipulate flowering time and develop new elite, high-yielding cultivars.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Both Jasmonic Acid- and Abscisic Acid-Mediated Signalling Pathways Regulate the Ovicidal Defence of Plants Against Phloem-Feeding Insects.

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-02-25 DOI: 10.1111/pce.15445

Qing Gao, Nuo Jin, Zhifan Shen, Jingran Guo, Haiping Lu, Shanjie Han, Wenhan Xiao, Jing Lu, Yonggen Lou

{"title":"Both Jasmonic Acid- and Abscisic Acid-Mediated Signalling Pathways Regulate the Ovicidal Defence of Plants Against Phloem-Feeding Insects.","authors":"Qing Gao, Nuo Jin, Zhifan Shen, Jingran Guo, Haiping Lu, Shanjie Han, Wenhan Xiao, Jing Lu, Yonggen Lou","doi":"10.1111/pce.15445","DOIUrl":"https://doi.org/10.1111/pce.15445","url":null,"abstract":"Plants perceive signals associated with herbivore eggs and in response initiate ovicidal defence. However, which phytohormone pathways regulate this defence and which defensive compounds dominate it remains largely unknown. Here, we found that the hatching rate of eggs of white-backed planthopper (WBPH) Sogatella furcifera was significantly lower on a japonica rice variety P81 than an indica rice variety NB44. When infested by WBPH, P81 plants showed higher jasmonic acid (JA) and abscisic acid (ABA) responses than did NB44 plants; moreover, P81 plants produced the ovicidal compound benzyl benzoate and exhibited higher levels of some flavonoids, phenolamides, and volatiles than were found in NB44 plants. Impairing the ABA-signalling pathway, especially the JA-signalling pathway in P81 plants enhanced the survival of WBPH eggs. Decreasing levels of some flavonoids and phenolamides in P81 plants promoted WBPH egg survival. In vitro bioassays revealed that both naringenin and sakuranetin promote the ovicidal effect of benzyl benzoate on WBPH. The results demonstrate that JA- and ABA-signalling pathways jointly regulate the rice ovicidal defence against WBPH, and that benzyl benzoate, as well as some other compounds, such as naringenin and sakuranetin, contribute to the mortality of WBPH eggs.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elicitor Specific Mechanisms of Defence Priming in Oak Seedlings Against Powdery Mildew.

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-02-25 DOI: 10.1111/pce.15419

Rosa Sanchez-Lucas, Jack L Bosanquet, James Henderson, Marco Catoni, Victoria Pastor, Estrella Luna

{"title":"Elicitor Specific Mechanisms of Defence Priming in Oak Seedlings Against Powdery Mildew.","authors":"Rosa Sanchez-Lucas, Jack L Bosanquet, James Henderson, Marco Catoni, Victoria Pastor, Estrella Luna","doi":"10.1111/pce.15419","DOIUrl":"https://doi.org/10.1111/pce.15419","url":null,"abstract":"Defence priming sensitises plant defences to enable a faster and/or stronger response to subsequent stress. Various chemicals can trigger priming; however, the response remains unexplored in oak. Here, we characterise salicylic acid (SA)-, jasmonic acid (JA)-, and β-aminobutyric acid (BABA)-induced priming of oak seedlings against the causal agent of powdery mildew (Erysiphe alphitoides, PM). Whilst JA had no effects, BABA and SA enhanced resistance by priming callose deposition and SA-dependent gene expression, respectively. Untargeted transcriptome and metabolome analyses revealed genes and metabolites uniquely primed by BABA, SA, and JA. Enrichment analyses demonstrated a limited number of pathways differentiating the three treatments or the resistance-inducing elicitors BABA and SA. However, a similar mode of action between BABA and JA was identified. Moreover, our analyses revealed a lack of crosstalk between SA and JA. Interestingly, priming by BABA was linked to alkaloid, lignan, phenylpropanoid, and indolitic compounds biosynthesis. Moreover, integration of the omics analyses revealed the role of ubiquitination and protein degradation in priming by BABA. Our results confirm the existence of chemical-induced priming in oak and has identified specific molecular markers associated with well-characterised elicitors.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143497741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR3398-VqMYB15 Regulates the Synthesis of Stilbene in Vitis quinquangularis. miR3398-VqMYB15 调控五角葡萄中芪的合成

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-02-24 DOI: 10.1111/pce.15446

Yangyang Luo, Yang Feng, Qingqing Xie, Wenjing Lu, Dong Duan

{"title":"miR3398-VqMYB15 Regulates the Synthesis of Stilbene in Vitis quinquangularis.","authors":"Yangyang Luo, Yang Feng, Qingqing Xie, Wenjing Lu, Dong Duan","doi":"10.1111/pce.15446","DOIUrl":"https://doi.org/10.1111/pce.15446","url":null,"abstract":"Grapevine (Vitis vinifera L.) is an economically important fruit crop grown worldwide. Grapevine is cultivated extensively in China, and certain wild grapes exhibit excellent resistance to pathogens and stress. MicroRNAs (miRNAs) act as key regulators of plant growth, development, and immunity; however, their functions in grape stilbene synthesis are poorly understood. We identified an miRNA (miR3398) that negatively regulates the transcription factor MYB15 and participates in the synthesis of stilbene from Vitis quinquangularis (V. quinquangularis). MiR3398 and VqMYB15 showed completely opposite expression patterns after AlCl3 treatment, and the interaction between miR3398 and VqMYB15 was confirmed using 5'-RACE ligase-mediated rapid amplification of cDNA ends, dual-luciferase reporter gene system, and western blot analysis. VqMYB15 could bind to the VqSTS48 promoter by using yeast one-hybrid and electrophoretic mobility shift assay, and overexpression of VqMYB15 promoted stilbene accumulation in grape leaves. Using an overexpression and silencing system, we found that miR3398 negatively targets VqMYB15 to synthesis of stilbenes. We used Al3+ as an elicitor, indicating that miR3398 plays an important role in the plant immunity of V. quinquangularis. We also found that miR3398 is involved in plant immunity by detecting its promoter activity in grape protoplasts, luciferase imaging, and transgenic Arabidopsis thaliana. More importantly, we found that an ethylene transcription factor, ERF057, can bind to the promoter of miR3398 using Y1H and EMSA assays and inhibit its transcription using DLR, luciferase imaging, and β-glucuronidase transcript assays. Overexpression of VqERF057 reduced miR3398 transcript in V. quinquangularis and transgenic grapevine calli, but increased the stilbene content. These findings contribute to the understanding of the biological functions of miR3398 regulates stilbene synthesis in grapevines and clarify the molecular mechanism underlying the interaction between miR3398 and VqMYB15.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Network Architecture of Leaf Trait Correlations Has Shifted Following Crop Domestication. 作物驯化后叶片性状相关性的网络结构发生了变化

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-02-24 DOI: 10.1111/pce.15443

Zhangying Lei, Ziliang Li, Ian J Wright, Shubham Chhajed, Wangfeng Zhang, Daohua He, Yali Zhang

{"title":"Network Architecture of Leaf Trait Correlations Has Shifted Following Crop Domestication.","authors":"Zhangying Lei, Ziliang Li, Ian J Wright, Shubham Chhajed, Wangfeng Zhang, Daohua He, Yali Zhang","doi":"10.1111/pce.15443","DOIUrl":"https://doi.org/10.1111/pce.15443","url":null,"abstract":"Domestication of crops with the goal of improving yield has led to spectacular shifts in phenotypic traits and their correlation patterns. However, it is relatively unknown whether domestication has driven variation in the architecture of trait correlation networks to optimise carbon return on construction cost along the leaf economics spectrum (LES). Here, we compiled a data set of leaf functional, biochemical, and anatomical traits of 54 wild and cultivated crops. We found that crops tended to be located at the acquisitive end of global LES, typically having low leaf mass per area (LMA), high photosynthetic rate per mass, and high leaf nitrogen and phosphorus content per mass. Architectural changes in trait networks (module number, hub traits, and number of correlations) aligned with the notion of a divergent domestication syndrome due to artificial selection for faster growth and higher yield in high-resource agricultural fields. Domestication has increased the carbon return on resource investment via selecting trait combinations including higher photosynthesis, greater leaf area and LMA. We highlight that strategy-shifts towards faster photosynthetic return on investments in leaves have been coordinated with divergent trait correlation, which has important implications for understanding patterns of trait covariation under crop domestication.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SmDREB A1-10 Is Required for SmTTF30-Mediated Hypoxia Stress Tolerance in Salix matsudana.

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-02-24 DOI: 10.1111/pce.15442

Yanhong Chen, Mingchao Deng, Qianhui Huang, Guoyuan Liu, Jian Zhang

{"title":"SmDREB A1-10 Is Required for SmTTF30-Mediated Hypoxia Stress Tolerance in Salix matsudana.","authors":"Yanhong Chen, Mingchao Deng, Qianhui Huang, Guoyuan Liu, Jian Zhang","doi":"10.1111/pce.15442","DOIUrl":"https://doi.org/10.1111/pce.15442","url":null,"abstract":"Frequent flooding events induced by extreme weather significantly threaten plant growth and productivity. Salix matsudana, a willow species, demonstrates exceptional tolerance to hypoxia and submergence stress, providing an ideal model for exploring underlying molecular mechanisms. This study highlights the roles of two transcriptional factors and their interplay in enhancing hypoxia and submergence stress resilience in Salix matsudana. SmTTF30, a GT-1 trihelix transcription factor, is specifically induced under root hypoxia, with its promoter enriched in hypoxia-responsive elements. Functional analyses reveal that overexpression of SmTTF30 in Arabidopsis thaliana improves submergence tolerance, whereas its downregulation in Salix matsudana results in heightened submergence stress sensitivity. SmDREB A1-10, identified through yeast one-hybrid screening and dual-luciferase assays as an upstream regulator of SmTTF30, directly interacts with its promoter. Overexpression of SmDREB A1-10 in Arabidopsis thaliana also enhances submergence tolerance, similar to SmTTF30. Virus-induced gene silencing (VIGS) experiments confirm that silencing SmDREB A1-10 diminishes SmTTF30 expression and hypoxia-responsive gene activation, exacerbating submergence stress effects. These findings unveil a regulatory cascade involving SmDREB A1-10 and SmTTF30 in submergence stress responses, providing insights into transcriptional networks governing submergence tolerance in trees.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsMED25-OsWRKY78 Mediated Transcriptional Activation of OsGA20ox1 Positively Regulates Plant Height in Rice.

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-02-24 DOI: 10.1111/pce.15441

Yonghui Miao, Chenxiao Xu, Ye Zhang, Huapeng Zhou, Qian Xu

{"title":"OsMED25-OsWRKY78 Mediated Transcriptional Activation of OsGA20ox1 Positively Regulates Plant Height in Rice.","authors":"Yonghui Miao, Chenxiao Xu, Ye Zhang, Huapeng Zhou, Qian Xu","doi":"10.1111/pce.15441","DOIUrl":"https://doi.org/10.1111/pce.15441","url":null,"abstract":"Plant height is a crucial agronomic trait affecting lodging resistance and yield. The mediator subunit, such as MED25, plays a crucial role in regulating plant growth and development. This study elucidated the mechanistic role of OsMED25, an integral subunit of the plant mediator transcriptional coactivator complex, in the regulation of plant height. Phenotypic results indicated a significant reduction in plant height in the OsMED25-RNAi line. Further analysis indicated that GA1 and GA3 levels were significantly reduced, and the expression of gibberellin biosynthesis-related genes OsGA20ox1, OsGA20ox2 and OsGA20ox8 was significantly downregulated. Additionally, multiple lines of evidence supported an interaction between OsMED25 and OsWRKY78. The oswrky78 mutants exhibited significantly reduced plant height, and molecular analysis demonstrated that OsWRKY78 directly binds to the promoter region of OsGA20ox1 to activate its expression. Intriguingly, we demonstrated that OsMED25 acted as a coactivator for OsWRKY78, enhancing the transcription of OsGA20ox1. This led to elevated GA levels, positively regulating plant height. In summary, these findings demonstrated that OsMED25 played a pivotal role in regulating plant height by modulating the expression of OsGA20ox1, thereby providing a potential strategy for genetic improvement in rice.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strigolactones, ROS and ABA Regulate Systemic Salt-Tolerance Priming Signals Between Dodder-Connected Tobacco Plants.

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-02-21 DOI: 10.1111/pce.15438

Xijie Zheng, Jingxiong Zhang, Man Zhao, Zhongxiang Su, Hongjing Li, Jianqiang Wu

{"title":"Strigolactones, ROS and ABA Regulate Systemic Salt-Tolerance Priming Signals Between Dodder-Connected Tobacco Plants.","authors":"Xijie Zheng, Jingxiong Zhang, Man Zhao, Zhongxiang Su, Hongjing Li, Jianqiang Wu","doi":"10.1111/pce.15438","DOIUrl":"https://doi.org/10.1111/pce.15438","url":null,"abstract":"The parasitic plants dodders (Cuscuta spp., Convolvulaceae) can often simultaneously parasitize two or more neighbouring hosts, forming dodder-connected plant clusters. In a dodder-connected plant cluster, salt-induced systemic priming signals are transferred from the salt-stressed host (signal donor, SD) to the other host (signal receiver, SR) through dodder and prime the SR plants for enhanced salt tolerance, but what signalling pathways regulate the dodder-mediated interplant priming signals remain unclear. In this study, using genetic analyses, we show that in dodder-connected tobacco (Nicotiana tabacum) clusters, the strigolactone (SL), reactive oxygen species (ROS) and abscisic acid (ABA) pathway in the SD plants negatively control the salt stress-induced systemic signals from SD to SR plants. Transcriptome data suggested that the salt-induced systemic signals regulated by SLs in the SD plants may also affect the ABA and ROS signalling pathway in the SR plants. Quantification of the ABA and H2O2 contents in the SD plants suggested that the SL and ROS signalling likely converge on the ABA pathway to regulate the priming signals. This study reveals the important regulatory roles of phytohormones and ROS in dodder-mediated interplant communications and provides new insight into systemic signalling during salt stress adaptation in individual plants.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VvprePIP, the Precursor of a PAMP-Induced Secreted Peptide, Stimulates Defence Responses and Improves Resistance to Plasmopora viticola in Grapevine.

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-02-21 DOI: 10.1111/pce.15439

Huimin Huang, Jiaqi Liu, Wei Wu, Jiang Lu

{"title":"VvprePIP, the Precursor of a PAMP-Induced Secreted Peptide, Stimulates Defence Responses and Improves Resistance to Plasmopora viticola in Grapevine.","authors":"Huimin Huang, Jiaqi Liu, Wei Wu, Jiang Lu","doi":"10.1111/pce.15439","DOIUrl":"https://doi.org/10.1111/pce.15439","url":null,"abstract":"PRRs (Pattern-Recognition Receptors) distributed on plant cell membranes recognize not only PAMPs (Pathogen-Associated Molecular Patterns) released from the pathogens but also ligand peptides secreted from the plants, followed by eliciting defence responses. Here, we demonstrate that transcription of VvprePIP from grape (Vitis vinifera) encoding the precursor of a PIP (PAMP-Induced secreted Peptide) peptide is activated by Plasmopara viticola infection. Overexpression of VvprePIP increases the expression of defence-related genes and ROS (Reactive Oxygen Species) production, enhancing resistance to P. viticola in V. vinifera. A WRKY transcription factor VvWRKY8 interacts with VvprePIP promoter, upregulating its transcription directly. The immune reactions resulting from ectopic expression of VvprePIP are impaired in NbBAK1-silencing tobacco, implying BAK1 is necessary for the recognition between mature peptide VvPIP and its receptor. The conserved region at the C terminus of VvprePIP carries three typical SGPS-GH motifs, all of which contribute to provoke immune responses in plant. As synthetic VvPIP with a hydroxylated modification at the forth proline can mimic the functions of overexpression of the precursor, while synthetic unmodified VvPIP cannot, we reported that hydroxyproline is required for VvPIPs to serve as an active signal molecular. In conclusion, our studies reveal that VvprePIP plays a role in enhancing plant resistance to pathogens.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Conserved Endonuclease From Rust Fungi Suppresses Plant Extracellular DNA-Triggered Immunity.

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-02-20 DOI: 10.1111/pce.15440

Ying Zhang, Zhenkai Hao, Jiting Nian, Yanfei Zhang, Song Tian, Yiqian Xu, Wanlu Duan, Zhensheng Kang, Jing Zhao

{"title":"A Conserved Endonuclease From Rust Fungi Suppresses Plant Extracellular DNA-Triggered Immunity.","authors":"Ying Zhang, Zhenkai Hao, Jiting Nian, Yanfei Zhang, Song Tian, Yiqian Xu, Wanlu Duan, Zhensheng Kang, Jing Zhao","doi":"10.1111/pce.15440","DOIUrl":"https://doi.org/10.1111/pce.15440","url":null,"abstract":"Nuclease 1 (NUC1) is a highly conserved nonspecific endonuclease present in vertebrates, fungi, and a few plant species. While the involvement of yeast NUC1p in apoptotic cell death independent of metacaspase or apoptosis-inducing factors is documented, its function in other fungi, particularly pathogenic ones, remains elusive. In this study, we identified and characterised the homologue of yeast NUC1p, termed PstNUC1, in Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust. PstNUC1 was induced during infection and was secreted to the extracellular space of the host. Silencing of PstNUC1 significantly attenuated the virulence of Pst, suggesting its critical role in pathogenicity. Exogenous treatment of PstNUC1 diminished the extracellular DNA (exDNA)-triggered plant immune response, including cell death, oxygen peroxide production, and upregulation of Pathogenesis-related genes. Notably, overexpression of wild-type PstNUC1, but not a signal peptide-deficient mutant (PstNUC1ΔSP), in wheat compromised exDNA-triggered immunity, resulting in enhanced susceptibility to Pst infection. These finding collectively highlight the contribution of PstNUC1 to virulence through degradation of exDNA, thereby dampening the exDNA-induced plant immune response.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0