{"title":"Drought and Herbivory Drive Physiological and Phytohormonal Changes in Soybean (Glycine max Merril): Insights From a Meta-Analysis.","authors":"Manish Gautam, Rupesh Kariyat","doi":"10.1111/pce.15558","DOIUrl":"https://doi.org/10.1111/pce.15558","url":null,"abstract":"<p><p>With climate change, abiotic and biotic stresses such as drought and herbivory are predicted to further diminish agricultural productivity. Soybean (Glycine max [L.] Merrill), a crop of global economic importance, is vulnerable to both. However, the interactive effects of drought and herbivory on soybeans haven't been explored, especially through the lens of physiological and phytohormonal changes. To address this, we conducted a meta-analysis from 114 published studies, further reduced to 31 after removing redundancy, with data on physiological (photosynthesis and stomatal conductance) and phytohormonal traits (jasmonic acid [JA] and salicylic acid [SA]) of soybeans under drought, herbivory, and their combination. With existing studies so far, we show that drought has negative impacts on soybean photosynthesis and stomatal conductance whereas herbivory has negative effects on photosynthesis but neutral effects on stomatal conductance. And the effects of drought and herbivory on phytohormones had opposite effects, with JA levels increasing. To our understanding, this is the first meta-analysis assimilating studies to understand the combined impacts of drought and herbivory on soybean physiological and phytohormonal changes. We also put forward potential questions and avenues for further research in expanding our understanding about the regulatory pathways and consequences of other abiotic and biotic stressors in agroecosystems.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Protoplast Cell-Wall Regeneration: Unlocking New Potential for Genetic Improvement and Tree Breeding.","authors":"Qizouhong He, Yilan E, Ruili Li","doi":"10.1111/pce.15537","DOIUrl":"https://doi.org/10.1111/pce.15537","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"OsEPSPS Balances Disease Resistance and Plant Growth.","authors":"Kaiwei He, Liting Xu, Xiaobo Zhu, Wei Zhang, Lifen Ren, Yuchen Liu, Chunyu Guo, Yahuan Ma, Qian Yi, Yingzhi Xu, Junjie Yin, Xiumei Luo, Lijuan Zou, Li Song, Xiang Lu, Yongyan Tang, Min He, Xuewei Chen, Weitao Li","doi":"10.1111/pce.15565","DOIUrl":"https://doi.org/10.1111/pce.15565","url":null,"abstract":"<p><p>The balance between the antagonistic traits, such as plant growth and disease resistance, is crucial for developing elite crop varieties. While the roles of plant hormones in this balance are well established, the regulatory function of secondary metabolites remains largely unexplored. Here, we report that 5-enolpyruvylshikimate-3-phosphate synthase (OsEPSPS), a key enzyme in the shikimate pathway, regulates both plant growth and disease resistance. Silencing the OsEPSPS gene in rice compromises the shikimate pathway but enhances the nicotinate and nicotinamide metabolism, resulting in the accumulations of trigonelline and nicotinamide mononucleotide (NMN). These metabolites boost resistance to rice blast by activating plant immune responses rather than inhibiting the germination and growth of Magnaporthe oryzae. Furthermore, silencing OsEPSPS conferring disease resistance results in less growth in plant. Our findings highlight the pivotal role of OsEPSPS in coordinating plant growth and disease resistance.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meng Li, Ying Fu, Xinyue Liang, Xueting Liu, Li Huang, Sen Zhang, Wei Zhang, Kexuan Tang, Qian Shen
{"title":"SlHDZIV3 and SlHDZIV9 Are Two Positive Regulators in the Formation of Tomato Trichomes.","authors":"Meng Li, Ying Fu, Xinyue Liang, Xueting Liu, Li Huang, Sen Zhang, Wei Zhang, Kexuan Tang, Qian Shen","doi":"10.1111/pce.15560","DOIUrl":"https://doi.org/10.1111/pce.15560","url":null,"abstract":"<p><p>Trichomes serve as the first line of defense for plants against biotic and abiotic stress. The HD-ZIP IV family plays conserved roles in regulating the development of trichomes in different species, but its specific regulatory mechanism in tomato is not fully understood. In this study, we demonstrate that SlHDZIV3 and SlHDZIV9 are positive regulators of trichome development in tomato, and they function in a partially redundant yet distinct manner. SlHDZIV3 primarily affects the formation of long trichomes on tomato leaves and early formed leaf petiole, while SlHDZIV9 influence the formation of long trichomes across a broader range of tissues, including the fruit trichomes. The CRISPR/Cas9 mutants of SlHDZIV9 exhibit a reduction in fruit trichomes, leading to an extended postharvest shelf-life of tomato fruit. In addition, both SlHDZIV3 and SlHDZIV9 are essential for jasmonic acid-mediated regulation of long trichomes formation. This study enhances our understanding of trichome initiation and development in tomato and provides new strategies for improving postharvest preservation of tomato fruits.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Delineating microRNA169-Nuclear Factor Y-Subunit A Module for Its Potential Implications in Crop Improvement.","authors":"Anirban Chakraborty, Shambhavi Sharma, Girdhar K Pandey, Sabhyata Bhatia, Manoj Prasad","doi":"10.1111/pce.15555","DOIUrl":"https://doi.org/10.1111/pce.15555","url":null,"abstract":"<p><p>Climate change considerably impacts plant growth and productivity by inducing stress responses. This, along with the problems of feeding the ever-increasing global population, could be mitigated by generating climate-resilient crop varieties with enhanced productivity. However, an exhaustive account of the key regulatory processes that underlie developmental and stress-responsive pathways is a prerequisite for generating improved crop varieties. Towards this, our study, for the first time, provides an exhaustive compilation of the potential regulatory pathways impacted by the miR169-NFYA network in plants. The NFYA transcription factors belong to a class of nuclear factor-encoding genes directly influencing the transcription of many genes involved in developmental and stress responses. Meanwhile, miR169 provides a layer to NFYA-mediated gene regulation by post-transcriptionally suppressing the expression of these transcription factors. Evidence from several studies shed light on key molecular signatures related to hormone synthesis and signaling, calcium signaling, epigenetic regulation, nutrient starvation and miRNA biogenesis that could serve as downstream components of the miR169-NFYA cascade in plants. This ability of miR169-NFYA nexus to impact a wide range of biological processes makes it a suitable toolbox for developing tailor-made crop varieties through appropriate genetic manipulation strategies.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jingli Ding, Chenchen Ji, Chuang Wang, Sheliang Wang, Guangda Ding, Lei Shi, Fangsen Xu, Hongmei Cai
{"title":"OsMYB67 Knockout Promotes Rice Heading and Yield by Facilitating Copper Distribution in Panicles.","authors":"Jingli Ding, Chenchen Ji, Chuang Wang, Sheliang Wang, Guangda Ding, Lei Shi, Fangsen Xu, Hongmei Cai","doi":"10.1111/pce.15540","DOIUrl":"https://doi.org/10.1111/pce.15540","url":null,"abstract":"<p><p>Copper (Cu) is an essential micronutrient required for rice flowering and seed setting. Here, we identified that Cu-induced R2R3-MYB transcription factor, OsMYB67, acts as a negative regulator that controls rice heading and yield production by affecting Cu distribution in panicles. OsMYB67 was constitutively expressed, with the highest expression in the roots. OsMYB67 knockout did not affect plant growth, but significantly increased Cu concentrations in roots, shoots, and xylem sap at the seedling stage. At the reproductive stage, OsMYB67 mutants displayed an early heading phenotype, with significantly increased Cu distribution in panicles but decreased Cu distribution in leaves, whereas OsMYB67-overexpressing plants showed the opposite result. In addition, higher grain yield and Cu concentrations in seeds were observed in OsMYB67 mutants compared to the wild-type. The results of Y1H, transient co-expression, EMSA, in situ RT-PCR, and RT-qPCR showed that OsMYB67 directly binds to the promoter region of OsHMA9 and upregulates its expression. Significantly increased Cu concentrations were also observed in the roots, shoots, and seeds of oshma9 mutants, consistent with the results observed in OsMYB67 mutants. Interestingly, dramatically higher expression levels of OsATX1 and OsYSL16 were observed in the OsMYB67 mutants, which may contribute to the increased Cu distribution in the panicles.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qiang Yao, Jie Dong, Tengyue Zhang, Huihui Cao, Jie Yu, Xu Wang, Bingyao Li, Lin Zhu, Yuhua Wang, Aigen Fu, Fei Wang
{"title":"Dimerization of Immunophilin CYN38 Regulates Photosystem II Repair In Chlamydomonas.","authors":"Qiang Yao, Jie Dong, Tengyue Zhang, Huihui Cao, Jie Yu, Xu Wang, Bingyao Li, Lin Zhu, Yuhua Wang, Aigen Fu, Fei Wang","doi":"10.1111/pce.15556","DOIUrl":"https://doi.org/10.1111/pce.15556","url":null,"abstract":"<p><p>The high light (HL) tolerance of Chlamydomonas determines biomass productivity under excess light conditions. The repair cycle of photosystem II (PSII) is a fundamental process that ensures long-term HL adaptation in photosynthetic organisms. Immunophilins, originating from cyanobacteria and surged in eukaryotic photosynthetic species, were characterized to play pivotal functions for HL adaptation by influencing PSII activity directly or indirectly. Here, we identified that Chlamydomonas immunophilin CYN38, the conserved homolog of Arabidopsis CYP38, was localized in the thylakoid lumen. One intriguing cyn38 mutant caused by the insertion mutation to produce a longer protein CYN38(L) with an extended C terminus was characterized. The cyn38 mutant displayed HL sensitive phenotype, with dramatically reduced accumulation of PSII supercomplexes and PSII core subunits under HL treatment. In WT, CYN38 forms a homodimer relying on its C terminus and associates with PSII complexes. In cyn38, the CYN38(L) protein can neither dimerize nor associate with PSII complexes, which causes defective PSII repair. Taken together, our work demonstrated the conserved physiological function of CYN38 during PSII biogenesis in photosynthetic species and unraveled a previously unidentified dimerization of CYN38 for its function in PSII repair under HL stress.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bin Zhang, Tuuli-Marjaana Koski, Hualing Wang, Zhenzhu Chen, Huiping Li, Judith Mogouong, Kathryn E Bushley, Longsheng Xing, Jianghua Sun
{"title":"The Role of Phenylpropanoids and the Plant Microbiome in Defences of Ash Trees Against Invasive Emerald Ash Borer.","authors":"Bin Zhang, Tuuli-Marjaana Koski, Hualing Wang, Zhenzhu Chen, Huiping Li, Judith Mogouong, Kathryn E Bushley, Longsheng Xing, Jianghua Sun","doi":"10.1111/pce.15534","DOIUrl":"https://doi.org/10.1111/pce.15534","url":null,"abstract":"<p><p>Plants have coevolved with herbivorous insects for millions of years, resulting in variation in resistance both within and between species. Using a manipulative experiment combined with untargeted metabolomics, microbiome sequencing and transcriptomics approaches, we investigated the roles of plant metabolites and the microbiome in defence mechanisms in native resistant Manchurian ash (Fraxinus mandshurica) trees and non-native susceptible velvet ash (Fraxinus velutina) trees against the highly invasive emerald ash borer (EAB, Agrilus planipennis). Comparative transcriptomics and metabolomics analyses show that the phenylpropanoid pathway, which is enriched in differentially expressed genes and differentially abundant metabolites, may serve as a potential regulator of resistance. Additionally, the microbiome is distinctly shifted in two ash species. Indicator taxa analysis reveals that the distinct genera are dominant in the galleries of two ash species, for example, Pseudomonas in velvet, and Hafnia-Obesumbacterium in Manchurian. The strong correlation between indicator taxa and metabolites suggests that the chemical compounds might impact the microbial community in phloem directly or indirectly, or vice versa. This study significantly enhances our understanding of the variation in resistance between ash species and its contribution to the invasion success of EAB, providing valuable insights for the development of pest management strategies.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linlin Song, Tao Yang, Yakubu Saddeeq Abubakar, Yijuan Han, Ruina Zhang, Ya Li, Wenyu Ye, Guo-Dong Lu
{"title":"OsMbl1 Counteracts OsGdsl1-Mediated Rice Blast Susceptibility by Inhibiting Its Lipase Activity.","authors":"Linlin Song, Tao Yang, Yakubu Saddeeq Abubakar, Yijuan Han, Ruina Zhang, Ya Li, Wenyu Ye, Guo-Dong Lu","doi":"10.1111/pce.15552","DOIUrl":"https://doi.org/10.1111/pce.15552","url":null,"abstract":"<p><p>Plant lectins have a significant impact on the defense against pathogens and insect attacks. The jacalin-related lectin OsMbl1 from rice (Oryza sativa L.) has been reported to play a crucial role in pattern-triggered immunity (PTI). However, the underlying mechanism remains unclear. In this study, we identified a GDSL-like lipase, OsGdsl1, that interacts with OsMbl1 both in vitro and in vivo. The OsGdsl1 protein, which has lipase activity, is localized in the lipid bodies and apoplast. The expression of OsGDSL1 is modulated upon exposure to Magnaporthe oryzae (M. oryzae) or plant hormones. Deletion of the OsGDSL1 gene not only improved the resistance of rice to M. oryzae, but also led to an increased ROS burst after chitin treatments. The expression of some pathogenesis-related (PR) genes was upregulated in the mutants. We also found that OsMbl1 inhibited the lipase activity of OsGdsl1 during infection with M. oryzae. Overall, our results suggest that OsGdsl1 negatively regulates rice immunity to M. oryzae infection by downregulating ROS bursts and PR gene expressions, while its lipase activity, which is inhibited by OsMbl1, contributes to the enhancement of rice innate immunity during M. oryzae infection.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genome-Wide Identification and Functional Characterization of QdA3oGT Genes Reveal a Molecular Mechanism of Autumnal Leaf Color Transition in Quercus dentata.","authors":"Meng Jiang, Wen-Bo Wang, Yuhan Zhao, Xiangyue Wu, Shuchai Su, Cunfu Lu, Yazhou Zhao, Zenghui Hu, Pingsheng Leng","doi":"10.1111/pce.15545","DOIUrl":"https://doi.org/10.1111/pce.15545","url":null,"abstract":"<p><p>Autumnal leaf color transition is a crucial environmental adaptation strategy for trees. Quercus dentata is a typical autumn leaf tree species distributed in Northeast Asia, which is characterized by bright red leaves in autumn. However, the molecular mechanisms of leaf turning red respond to climatic factors are unclear. This study identified five anthocyanidin 3-O-glucosyltransferase (QdA3oGT) members, QdA3oGT2, with unique cis-elements, evolutionary background and gene structure, emerged as a key gene for leaf color transition. The transcript level of QdA3oGT2 was significantly higher than other members. qPCR revealed that the expression of QdA3oGT2 trend increased with leaf coloration, which was significantly positively correlated with anthocyanin content. Thus, QdA3oGT2 was identified as the key gene in leaf color transition of Q. dentata. Functional validation in Arabidopsis demonstrated that QdA3oGT2 was stimulated by low temperature, leading to enhanced cold resistance by anthocyanin accumulation. Furthermore, a cold-responsive and senescence-related transcription factor, QdNAC58 was identified, which directly bound to the NAC conserved binding motif 'ACGTCA' in QdA3oGT2 promoter, and positively regulated QdA3oGT2. In sum, this study reveals that QdNAC58, responds to low temperatures of autumn, further upregulates QdA3oGT2 to play a key role in anthocyanin synthesis associated with leaf senescence.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}