Plant, Cell & Environment最新文献_第9页

XsLTPG31 Confers Leaf Cuticular Wax Deposition and Drought Resistance in Yellowhorn. tpg31对黄角菊叶片表皮蜡沉积和抗旱性的影响。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-08-25 DOI: 10.1111/pce.70144

Huihui Xu, Xiaojuan Liu, Zhuo Ban, Yingying Yang, Lingfeng Zhang, Quanxin Bi, Libing Wang

{"title":"XsLTPG31 Confers Leaf Cuticular Wax Deposition and Drought Resistance in Yellowhorn.","authors":"Huihui Xu, Xiaojuan Liu, Zhuo Ban, Yingying Yang, Lingfeng Zhang, Quanxin Bi, Libing Wang","doi":"10.1111/pce.70144","DOIUrl":"https://doi.org/10.1111/pce.70144","url":null,"abstract":"With increasing frequency and severity under climate change, drought stress has become a predominant abiotic limiting factor of global plant productivity. Yellowhorn (Xanthoceras sorbifolium Bunge), an endemic species of woody oil tree in North China, has substantial developmental potential in arid and semi-arid regions. To elucidate the genetic basis of its drought response, a genome-wide association study (GWAS) of 13 leaf anatomical structure traits across 237 yellowhorn accessions was performed in this study, and 21 candidate genes that regulate leaf structural variation were identified. Among these genes, XsLTPG31, which encodes a nonspecific lipid transfer protein, was significantly induced by drought stress. Heterologous overexpression of XsLTPG31 in Arabidopsis, its transient overexpression in yellowhorn leaves, and virus-induced gene silencing (VIGS) of XsLTPG31 demonstrated that XsLTPG31 promotes the deposition of leaf epidermal wax and modulates drought resistance through facilitating the export of wax to the extracellular space. Moreover, we revealed that XsLTPG31 is directly activated by XsMYB16 via promoter binding. Taken together, the results of our study enhance the understanding of the regulatory mechanisms underlying LTPG-mediated cuticular wax deposition and might provide targets for the breeding of drought-tolerant varieties of yellowhorn.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Isotope Analysis Reveals Phylogenetic Patterns and Novel Insights Into Methoxy Group Synthesis of Structural Biomolecules in Leaf and Woody Plant Tissues. 双同位素分析揭示了叶和木本植物组织中结构生物分子甲氧基合成的系统发育模式和新见解。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-08-25 DOI: 10.1111/pce.70134

Anna Wieland, Philipp Schuler, Matthias Saurer, Valentina Vitali, Markus Greule, Frank Keppler, Marco M Lehmann

{"title":"Dual Isotope Analysis Reveals Phylogenetic Patterns and Novel Insights Into Methoxy Group Synthesis of Structural Biomolecules in Leaf and Woody Plant Tissues.","authors":"Anna Wieland, Philipp Schuler, Matthias Saurer, Valentina Vitali, Markus Greule, Frank Keppler, Marco M Lehmann","doi":"10.1111/pce.70134","DOIUrl":"https://doi.org/10.1111/pce.70134","url":null,"abstract":"Stable carbon and hydrogen isotopes of wood methoxy groups (δ2Hmeth, δ13Cmeth), mainly sourced by structural biomolecules like lignin and pectin, provide important insights into climatic, hydrological and physiological conditions. This study systematically investigated species-specific δ2Hmeth and δ13Cmeth variations in leaves and woody twigs of 65 different tree species grown in a common garden. Significant phylogenetic patterns were observed in δ2Hmeth and δ13Cmeth of both tissues, with stronger signals in leaves and the most pronounced differences between angiosperms and gymnosperms. δ13Cmeth variations are likely explained by anatomical and physiological differences between seed types, while δ2Hmeth variations were attributed to temporal differences in water uptake or isotope fractionation processes. Notably, δ13Cmeth values were more negative in leaves than in twigs, while δ2Hmeth values showed no tissue-specific difference. This suggests that serine, a methoxy precursor, is differently synthesised in autotrophic than in heterotrophic tissues. Hydrogen isotope fractionation between xylem water and twig methoxy groups averaged at -197 mUr, with mean isotope fractionation of gymnosperms -209 mUr being significantly different to that of angiosperms -184 mUr. Weak relationships between δ2Hmeth and δ2H values of carbohydrates indicated that distinct signals are preserved within the two compounds. This study highlights the importance of phylogenetic considerations when using methoxy group isotopes as proxies and provides new insights into methoxy group biosynthesis.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chloroplasts Orchestrate Multilayered Defences Against Plant Pathogens. 叶绿体协调对植物病原体的多层防御。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-08-25 DOI: 10.1111/pce.70140

Qingqing Sun, Sijia Hou, Hongbo Gao

引用次数: 0

Role of CclA Loss in Parametarhizium changbaiense in Enhancing Priming Resistance of Mung Bean Against Damping Off Induced by Rhizoctonia solani. 长白参根芽孢杆菌CclA损失在提高绿豆抗枯枯性中的作用。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-08-25 DOI: 10.1111/pce.70145

Xinyu Xie, Chenxi Ma, Shan Liu, Ying Gao, Rui Cao, Yu Zhang, Fujuan Feng, Yanfeng Hu, Wei Meng, Lijian Xu

{"title":"Role of CclA Loss in Parametarhizium changbaiense in Enhancing Priming Resistance of Mung Bean Against Damping Off Induced by Rhizoctonia solani.","authors":"Xinyu Xie, Chenxi Ma, Shan Liu, Ying Gao, Rui Cao, Yu Zhang, Fujuan Feng, Yanfeng Hu, Wei Meng, Lijian Xu","doi":"10.1111/pce.70145","DOIUrl":"https://doi.org/10.1111/pce.70145","url":null,"abstract":"Rhizoctonia solani is a soil-borne pathogen causing destructive diseases on various economically important crops. Beneficial plant and fungus interactions are well acknowledged to enhance plant resistance to biotic stresses. However, the underlying mechanisms are not fully understood, especially the contribution of fungal epigenetic regulation remains poorly understood. Here, we characterized Parametarhizium changbaiense as a beneficial fungus that promoted mung bean growth and priming resistance to damping off caused by R. solani. Knockout of CclA, a subunit of the COMPASS complex involved in H3K4 methylation, reduced the H3K4me1 and 3 levels in P. changbaiense and diminished the fungal growth and conidiation. However, PcCclA deletion enhanced the root colonisation and further bolstered disease resistance, which correlated with intensified lignin content, lignification in the stem cells, and reactive oxygen species (ROS) scavenging ability, indicating a boost in priming defense responses. Taken together, our findings not only supported a conserved function of PcCclA in fungal growth regulation, but also demonstrated the role of PcCclA-mediated H3K4 methylation in host defense priming, which deepens the understanding of the importance of fungal epigenetic regulation in the plant-beneficial fungus interaction. Meanwhile, we also provided a strategy to enhance priming defense response through epigenetic engineering of plant-beneficial fungi.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights Into the Role of Lysine Acetylation of Non-Histone Proteins in Plant Immunity. 非组蛋白赖氨酸乙酰化在植物免疫中的作用

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-08-21 DOI: 10.1111/pce.70139

Jérémy Villette, Chloé Ilbert, Sébastien Aimé, Hoai-Nam Truong, Marielle Adrian, Stéphane Bourque

{"title":"Insights Into the Role of Lysine Acetylation of Non-Histone Proteins in Plant Immunity.","authors":"Jérémy Villette, Chloé Ilbert, Sébastien Aimé, Hoai-Nam Truong, Marielle Adrian, Stéphane Bourque","doi":"10.1111/pce.70139","DOIUrl":"https://doi.org/10.1111/pce.70139","url":null,"abstract":"Plant immunity is regulated by numerous transcriptional and posttranslational mechanisms. Among these, lysine acetylation, which is controlled by lysine acetyltransferases (KATs) and lysine deacetylases (KDACs), has been extensively studied, particularly in the context of epigenetic regulation through histone acetylation. However, advances in proteomics have revealed that non-histone proteins also undergo lysine acetylation, prompting increasing efforts to elucidate the underlying mechanisms and functions of this posttranslational modifications. This review provides a comprehensive analysis of acetyl-lysine proteome (acetylome) studies during plant interaction with pathogens (including fungi, bacteria and viruses). By highlighting the significance of lysine acetylation in non-histone proteins, these studies offer valuable insights into potential new targets for lysine acetylation in plant immunity. We further examine the roles of plant KATs and KDACs, as well as pathogen-derived KATs, emphasizing the different types of lysine acetylation in non-histone proteins. In particular, we explore how bacterial effectors, which mimic KAT activity, acetylate lysine residues in non-histone proteins to modulate plant immunity. Additionally, we discuss on emerging molecular mechanisms involving plant KATs and KDACs that finely regulate immune responses, particularly within the jasmonic acid signalling pathway. These findings open new perspectives for future research on this posttranslational regulation within the context of plant immunity.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diurnal Variations in Photochemical Energy Utilization and Osmotic Adjustments in Black Poplar Leaves Under Progressive Water Stress. 进行性水分胁迫下黑杨叶光化学能利用及渗透调节的日变化

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-08-21 DOI: 10.1111/pce.70135

Antonella Gori, Mauro Centritto, Anatoly P Sobolev, Giovanni Marino, Francesco Loreto, Francesca Alderotti, Cecilia Brunetti

{"title":"Diurnal Variations in Photochemical Energy Utilization and Osmotic Adjustments in Black Poplar Leaves Under Progressive Water Stress.","authors":"Antonella Gori, Mauro Centritto, Anatoly P Sobolev, Giovanni Marino, Francesco Loreto, Francesca Alderotti, Cecilia Brunetti","doi":"10.1111/pce.70135","DOIUrl":"https://doi.org/10.1111/pce.70135","url":null,"abstract":"Drought limits the productivity of fast-growing woody crops, although the metabolic adjustments conferring water stress tolerance remain poorly understood. We investigated the responses of Populus nigra seedlings to water stress by integrating daily physiological measurements and NMR metabolomic analyses. Our aims were to: (i) determine key metabolic and biochemical responses in leaves subjected to moderate (WS1) and severe (WS2) water stress and (ii) identify the metabolites responsible for dissipating excess photochemical energy and maintaining cellular turgor. Despite the reduction in electron transport rate, photoprotective mechanisms were activated. A threefold increase in isoprene emission was observed at midday in WS1 plants, while ascorbic acid and other antioxidants were higher under WS2 conditions. Relative water content and osmotic potential decreased throughout the day, suggesting that passive osmotic adjustments were primarily driven by soluble sugar accumulation. Organic acid reduction in WS1 (-9%) and WS2 (-17%) plants suggested an inhibition of the tricarboxylic acid cycle. Additionally, amino acids were twofold higher in water-stressed plants compared to controls, likely reflecting an increased demand for primary and secondary metabolite biosynthesis. Our results provide new insights into the daily response of P. nigra to water stress, highlighting a delicate balance between metabolic and physiological adjustments.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Double Strand Break Repair Is Important for the Longevity of Primed Seeds. DNA双链断裂修复对引物种子的寿命至关重要。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-08-21 DOI: 10.1111/pce.70142

Wanda M Waterworth, Dapeng Wang, Lerissa S Dsilva, Christopher E West

{"title":"DNA Double Strand Break Repair Is Important for the Longevity of Primed Seeds.","authors":"Wanda M Waterworth, Dapeng Wang, Lerissa S Dsilva, Christopher E West","doi":"10.1111/pce.70142","DOIUrl":"https://doi.org/10.1111/pce.70142","url":null,"abstract":"Germination of many crop species is improved by priming, which facilitates pre-germinative metabolism through controlled hydration. However, priming is often associated with reduced seed longevity. Here, a screen of Arabidopsis thaliana DNA repair mutants identified dna ligase 6 and dna ligase 4 (lig6lig4) seeds as most sensitive to ageing of primed seed. Genetic analysis of wild type and lig6lig4 mutants provided mechanistic insight into the link between DNA double strand break (DSB) repair and longevity of primed seeds. RNAseq analysis of naturally aged seeds demonstrated that, while the transcriptome changes in primed aged seeds mirrors the enhancement of germination, priming significantly activated the transcriptional response to chromosomal breaks and, in lig6lig4 mutant seed, greatly exacerbated programmed cell death. These results revealed that DSB repair is an important factor in promoting longevity of primed seed, further supported by the improved longevity of primed seeds with enhanced expression of LIG6. Collectively our findings establish the genetic requirement for LIG6 in longevity of primed seed and indicate that the reduced longevity of primed seeds is mitigated by DSB repair activities. These results provide insight into the molecular basis of the reduced longevity of primed seed, important for sustainable crop production under changing climates.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Fungal siRNA Is Involved in Genetic Robustness of VmRDR-Mediated Virulence in Valsa mali During Apple Infection. 一种真菌siRNA参与苹果侵染过程中由vmrdr介导的毒力的遗传稳健性

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-08-20 DOI: 10.1111/pce.70136

Jiahao Liang, Jie Wang, Peixin Wang, Ruixuan Fan, Hao Feng, Lili Huang

{"title":"A Fungal siRNA Is Involved in Genetic Robustness of VmRDR-Mediated Virulence in Valsa mali During Apple Infection.","authors":"Jiahao Liang, Jie Wang, Peixin Wang, Ruixuan Fan, Hao Feng, Lili Huang","doi":"10.1111/pce.70136","DOIUrl":"https://doi.org/10.1111/pce.70136","url":null,"abstract":"Genetic robustness refers to the ability of organisms to maintain normal phenotypes in the face of genetic variation, such as gene deletion. In plant pathogenic fungi, RNA-dependent RNA polymerases (RdRPs) play a crucial role in RNA interference (RNAi) signalling amplification in plant-pathogen interactions. However, the genetic robustness of RdRP-mediated fungal virulence and the molecular mechanisms regulating robustness remain elusive. In this study, we characterized the virulence genetic robustness following VmRDR3 knockout in Valsa mali during apple infection, and revealed an siRNA-mediated regulatory mechanism for genetic robustness. It was demonstrated that VmRDR3 knockout could induce compensatory upregulation of paralogous gene VmRDR2, which resulted in the stable abundance of sRNAs and the induction of new sRNAs generation, such as Vm-siR43. This siRNA specifically degrades MdWRKY3 (a disease resistance-related WRKY transcription factor gene in apple) in a sequence-specific manner, thereby suppressing host resistance. Concurrently, Vm-siR43 silences the fungal hypothetical protein gene VmHy5, impairing mycelial growth of V. mali. Our findings reveal a novel epigenetic regulation mechanism underlying RdRP-mediated fungal virulence robustness, operating through posttranscriptional gene silencing by a fungal siRNA. The results advance the understanding of the functional complexity of fungal RNAi components in coordinating pathogen adaptation and infection of the host.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The CgDbf2/CgMob1-CgCdc14 Phosphorylation Module Mediated Phosphatase Activity Regulates MEN Signalling and Pathogenicity in Colletotrichum gloeosporioides. CgDbf2/CgMob1-CgCdc14磷酸化模块介导的磷酸酶活性调控炭疽菌MEN信号传导和致病性

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-08-20 DOI: 10.1111/pce.70123

Jiyun Yang, Mei-Ling Sun, Yu-Ting Pan, Chuan-Xu Peng, Si-Qian Yuan, Ye-Dan Li, Jia-Hong Ren, Lin Huang

{"title":"The CgDbf2/CgMob1-CgCdc14 Phosphorylation Module Mediated Phosphatase Activity Regulates MEN Signalling and Pathogenicity in Colletotrichum gloeosporioides.","authors":"Jiyun Yang, Mei-Ling Sun, Yu-Ting Pan, Chuan-Xu Peng, Si-Qian Yuan, Ye-Dan Li, Jia-Hong Ren, Lin Huang","doi":"10.1111/pce.70123","DOIUrl":"https://doi.org/10.1111/pce.70123","url":null,"abstract":"The mitotic exit network (MEN) plays pivotal roles in regulating cell morphology and pathogenicity in eukaryotic organisms. However, the precise mechanisms underlying how the MEN signalling pathway integrates and coordinates morphologiensis, host derived ROS homoeostasis and virulence remains poorly understood in phytopathogenic fungi. In this study, combined with RNA-seq, biochemical and genetic analyses, we found that CgDbf2 and CgMob1 form a protein complex in Colletotrichum gloeosporioides. As an essential component of MEN signalling pathway, CgDbf2/CgMob1 complex is critical for maintaining cell wall integrity (CWI), and enabling resistance to the fungicide carbendazim. Disruption of CgDBF2 and CgMOB1 results in significantly reduced virulence, primarily due to impaired appressorium formation and diminished invasive growth capabilities. Additionally, our findings reveal that CgDbf2 interacts with and phosphorylates the phosphatase CgCdc14. The residues Ser427 and Thr428 of CgCdc14 are critical sites phosphorylated by CgDbf2, enhancing CgCdc14 phosphatase activity and contributing to fungal virulence. Moreover, our findings highlight that the Dbf2/Mob1-Cdc14 axis coordinates CWI and responses to reactive oxygen species, both of which are crucial for plant infection. Our data underscore that the phosphatase activity of CgCdc14, which is dependent on phosphorylation by CgDbf2, is essential for crosstalk between MEN and CWI. These findings offer novel insights into the molecular mechanisms governing plant infection of phytopathogenic fungi and provide potential targets for developing antifungal strategies.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural Variations in the Promoter of CHILLING TOLERANCE DIVERGENCE 8 Contribute to the Functional Divergence in the Chilling Tolerance of Rice. 抗寒性分化启动子的自然变异是水稻抗寒性功能分化的原因之一。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-08-20 DOI: 10.1111/pce.70122

Shuangshuang Zheng, Zhitao Li, Yanping You, Jie Cao, Wei Luo, Qian Qian, Yunyuan Xu, Kang Chong

{"title":"Natural Variations in the Promoter of CHILLING TOLERANCE DIVERGENCE 8 Contribute to the Functional Divergence in the Chilling Tolerance of Rice.","authors":"Shuangshuang Zheng, Zhitao Li, Yanping You, Jie Cao, Wei Luo, Qian Qian, Yunyuan Xu, Kang Chong","doi":"10.1111/pce.70122","DOIUrl":"https://doi.org/10.1111/pce.70122","url":null,"abstract":"Improvement of the chilling tolerance is one of the key points in rice breeding since it is highly sensitive to chilling stress, which often limits rice production. Here, we identified a quantitative trait locus for chilling tolerance, qCTS8, from a recombinant inbred line (RIL) population derived from crossing Dongxiang wild rice (DXWR) with Guangluai 4 (GLA4). Map-based cloning revealed CHILLING TOLERANCE DIVERGENCE 8 (COLD8) as the underlying major gene. RT-qPCR showed that the COLD8jap from japonica exhibited a significantly higher expression than the COLD8ind from indica. Transgenic lines demonstrated that COLD8 negatively regulated chilling tolerance at the seedling stage. Transactivation assays, yeast one-hybrid and EMSA suggested that the transcription factor OsbZIP23 directly bound to the ABA-responsive element (ABRE) of the COLD8 promoter, accounting for the higher expression of COLD8 in NIP than in GLA4. Evolutionary analysis indicated that the japonica allele COLD8jap originated from Chinese Oryza rufipogon and was selected during japonica domestication. COLD8 encodes a triacylglycerol lipase localized in chloroplast membrane. Loss-of-function mutants of COLD8 exhibited enhanced chilling tolerance associated with increased accumulation of unsaturated fatty acids 16:1 and 18:3. Together, these findings uncover a molecular link between lipid metabolism and chilling adaptation and establish COLD8 as a promising target for breeding chilling-tolerant rice.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0