Lu Li, Kexin Cheng, Yao Du, Yiwen Zhang, Yingwen Zhou, Yi Jin, Xiaoqing He
{"title":"胡杨根际微生物赋予白杨×腺杨抗盐胁迫能力。","authors":"Lu Li, Kexin Cheng, Yao Du, Yiwen Zhang, Yingwen Zhou, Yi Jin, Xiaoqing He","doi":"10.1111/pce.70160","DOIUrl":null,"url":null,"abstract":"<p><p>The rhizosphere microbiomes of halophytes are crucial for plant adaptation to high-salinity soil conditions, but how to harness rhizosphere microbes to confer salt stress resistance to plants remains obscure. This study aimed to establish a framework (isolate-select-construct) for tailoring simplified salt-tolerant synthetic microbial communities (SynComs) and explore how they confer salt stress resistance to the plant. First, a total of 512 strains were isolated from the high-salt rhizosphere soil of Populus euphratica through high-throughput cultivation. Among these, nine strains were further selected for their salt-tolerant and growth-promoting abilities, with three isolates identified as key microbes, including hub microbes, keystone taxa and biomarkers. Guided by a function-driven strategy, we constructed five distinct SynComs, with SynCom5, SynCom7 and SynCom9 showing the most significant improvement in the growth of hybrid Poplar 84K (Populus alba × Populus glandulosa). Mechanistic investigations revealed that these SynComs can increase resistance to salt stress by directly reducing oxidative stress, adjusting osmolytes and balancing ions. Additionally, these SynComs were observed to recruit specific root-associated bacterial consortia that enhance the adaptability of poplar to salt stress. Overall, this study lays the groundwork for designing SynComs that promote plant growth and offers insights into harnessing specific microbial communities to boost plants' salt resistance.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3000,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Rhizosphere Microbes From Populus euphratica Conferred Salt Stress Resistance to Populus alba × Populus glandulosa.\",\"authors\":\"Lu Li, Kexin Cheng, Yao Du, Yiwen Zhang, Yingwen Zhou, Yi Jin, Xiaoqing He\",\"doi\":\"10.1111/pce.70160\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The rhizosphere microbiomes of halophytes are crucial for plant adaptation to high-salinity soil conditions, but how to harness rhizosphere microbes to confer salt stress resistance to plants remains obscure. This study aimed to establish a framework (isolate-select-construct) for tailoring simplified salt-tolerant synthetic microbial communities (SynComs) and explore how they confer salt stress resistance to the plant. First, a total of 512 strains were isolated from the high-salt rhizosphere soil of Populus euphratica through high-throughput cultivation. Among these, nine strains were further selected for their salt-tolerant and growth-promoting abilities, with three isolates identified as key microbes, including hub microbes, keystone taxa and biomarkers. Guided by a function-driven strategy, we constructed five distinct SynComs, with SynCom5, SynCom7 and SynCom9 showing the most significant improvement in the growth of hybrid Poplar 84K (Populus alba × Populus glandulosa). Mechanistic investigations revealed that these SynComs can increase resistance to salt stress by directly reducing oxidative stress, adjusting osmolytes and balancing ions. Additionally, these SynComs were observed to recruit specific root-associated bacterial consortia that enhance the adaptability of poplar to salt stress. Overall, this study lays the groundwork for designing SynComs that promote plant growth and offers insights into harnessing specific microbial communities to boost plants' salt resistance.</p>\",\"PeriodicalId\":222,\"journal\":{\"name\":\"Plant, Cell & Environment\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":6.3000,\"publicationDate\":\"2025-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant, Cell & Environment\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://doi.org/10.1111/pce.70160\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant, Cell & Environment","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/pce.70160","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
盐生植物的根际微生物群对植物适应高盐土壤条件至关重要,但如何利用根际微生物赋予植物抗盐胁迫能力仍然是一个未知数。本研究旨在建立一个框架(分离-选择-构建),以定制简化的耐盐合成微生物群落(SynComs),并探索它们如何赋予植物耐盐性。首先,通过高通量培养,从胡杨高盐根际土壤中分离得到菌株512株。其中9株菌株具有耐盐和促生长能力,其中3株菌株被确定为关键微生物,包括枢纽微生物、关键分类群和生物标志物。在功能驱动策略的指导下,我们构建了5个不同的SynComs,其中SynCom5、SynCom7和SynCom9对杂交杨树84K (Populus alba × Populus glandulosa)的生长改善最为显著。机制研究表明,这些SynComs可以通过直接降低氧化应激、调节渗透物和平衡离子来增加对盐胁迫的抵抗力。此外,这些SynComs还可以招募特定的根相关菌群,从而增强杨树对盐胁迫的适应性。总的来说,这项研究为设计促进植物生长的SynComs奠定了基础,并为利用特定的微生物群落来提高植物的耐盐性提供了见解。
Rhizosphere Microbes From Populus euphratica Conferred Salt Stress Resistance to Populus alba × Populus glandulosa.
The rhizosphere microbiomes of halophytes are crucial for plant adaptation to high-salinity soil conditions, but how to harness rhizosphere microbes to confer salt stress resistance to plants remains obscure. This study aimed to establish a framework (isolate-select-construct) for tailoring simplified salt-tolerant synthetic microbial communities (SynComs) and explore how they confer salt stress resistance to the plant. First, a total of 512 strains were isolated from the high-salt rhizosphere soil of Populus euphratica through high-throughput cultivation. Among these, nine strains were further selected for their salt-tolerant and growth-promoting abilities, with three isolates identified as key microbes, including hub microbes, keystone taxa and biomarkers. Guided by a function-driven strategy, we constructed five distinct SynComs, with SynCom5, SynCom7 and SynCom9 showing the most significant improvement in the growth of hybrid Poplar 84K (Populus alba × Populus glandulosa). Mechanistic investigations revealed that these SynComs can increase resistance to salt stress by directly reducing oxidative stress, adjusting osmolytes and balancing ions. Additionally, these SynComs were observed to recruit specific root-associated bacterial consortia that enhance the adaptability of poplar to salt stress. Overall, this study lays the groundwork for designing SynComs that promote plant growth and offers insights into harnessing specific microbial communities to boost plants' salt resistance.
期刊介绍:
Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.