Decoding Xylem Development in Flowering Plants: Insights From Single-Cell Transcriptomics.

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Jhong-He Yu, Jo-Wei Allison Hsieh, Zhifeng Wang, Jia Wei, Quanzi Li, Ying-Lan Chen, Ying-Chung Jimmy Lin
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引用次数: 0

Abstract

Single-cell RNA sequencing (scRNA-seq) has emerged as a transformative tool for decoding plant development, particularly in elucidating xylem differentiation. By capturing transcriptomic changes at single-cell resolution, scRNA-seq enables reconstruction of developmental trajectories across diverse plant tissues. In this review, we summarize recent advances in the application of scRNA-seq to study both primary and secondary xylem development in monocots and eudicots. These studies have revealed distinct xylem cell types, including vessel elements, libriform fibers, and ray parenchyma cells, and provided insight into their lineage relationships. We also highlight key technical and analytical challenges that limit cross-study comparisons, including inconsistent bioinformatic pipelines, variability in protoplasting efficiency, and the use of potentially misannotated marker genes. To address these limitations, we discuss the integration of in situ transcriptomic profiling using laser microdissection, which provides more accurate cell-type annotation and supports the current best working model of xylem developmental lineages. Finally, we suggest future directions for improving xylem developmental studies, including deeper integration of spatial and single-cell technologies to overcome current limitations in resolving lignified tissues and to better understand xylem responses to environmental perturbations.

解码开花植物木质部发育:来自单细胞转录组学的见解。
单细胞RNA测序(scRNA-seq)已经成为解码植物发育的变革性工具,特别是在阐明木质部分化方面。通过捕获单细胞分辨率的转录组变化,scRNA-seq能够重建不同植物组织的发育轨迹。本文综述了近年来利用scRNA-seq技术研究单子房和双子房初生和次生木质部发育的研究进展。这些研究揭示了不同的木质部细胞类型,包括导管元件、libriform纤维和射线薄壁细胞,并为它们的谱系关系提供了见解。我们还强调了限制交叉研究比较的关键技术和分析挑战,包括不一致的生物信息管道,原生质体效率的可变性,以及使用可能错误注释的标记基因。为了解决这些限制,我们讨论了使用激光显微解剖的原位转录组分析的集成,它提供了更准确的细胞类型注释,并支持当前木质部发育谱系的最佳工作模型。最后,我们提出了未来木质部发育研究的发展方向,包括进一步整合空间和单细胞技术,以克服目前木质部对木质化组织的限制,并更好地了解木质部对环境扰动的反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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