Plant, Cell & Environment最新文献_第8页

A J-Domain Protein J3 Antagonizes ABI5-BINDING PROTEIN2 to Regulate CONSTANS Stability and Flowering Time. j结构域蛋白J3拮抗abi5结合蛋白2调控恒常蛋白稳定性和开花时间。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-03-12 DOI: 10.1111/pce.15469

LuLu Zhi, Heng Zhang, Yan Mao, Lei Liang, Huanhuan Ni, Ping Li, Jinling Huang, Ying Zhu, Xiangyang Hu

{"title":"A J-Domain Protein J3 Antagonizes ABI5-BINDING PROTEIN2 to Regulate CONSTANS Stability and Flowering Time.","authors":"LuLu Zhi, Heng Zhang, Yan Mao, Lei Liang, Huanhuan Ni, Ping Li, Jinling Huang, Ying Zhu, Xiangyang Hu","doi":"10.1111/pce.15469","DOIUrl":"https://doi.org/10.1111/pce.15469","url":null,"abstract":"CONSTANS (CO) plays a vital role in activating the expression of the florigen FT during photoperiod-dependent flowering. The diurnal oscillation of CO protein abundance is strictly controlled by daylength and the circadian clock. We showed previously that ABI5-BINDING PROTEIN2 (AFP2) represses CO expression to delay flowering. In this study, we identified DNAJ HOMOLOG 3 (J3) as an AFP2-interacting protein in Arabidopsis thaliana. The interaction between J3 and AFP2 was confirmed through a series of biochemical experiments. In long days, the j3 mutant exhibited pronounced late flowering with reduced CO protein abundance, whereas overexpressing J3 accelerated flowering time by stabilizing CO, suggesting that J3 acts as a positive regulator of photoperiod responses. Furthermore, J3 suppressed the interaction between CO and AFP2, and protected CO from AFP2-mediated degradation, thus efficiently activating FT expression at the end of the day. Consistent with these data, the late-flowering phenotype caused by overexpressing AFP2 was reversed by overexpressing J3 in planta, whereas the early-flowering phenotype of the afp2 mutant was compromised in the afp2 j3 double mutant. Together, these results reveal a novel function for J3 in coordinating CO stability through antagonizing AFP2 activity, thus fine-tuning the downstream FT expression during photoperiodic flowering.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal and Diurnal Transcriptome Atlas in Natural Environment Reveals Flowering Time Regulatory Network in Alfalfa. 自然环境中季节和昼夜转录组图谱揭示了紫花苜蓿开花时间调控网络。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-03-12 DOI: 10.1111/pce.15466

Yuanyuan Zhang, Haijun Zhao, Haoxin Xiang, Jiashuo Zhang, Lei Wang

{"title":"Seasonal and Diurnal Transcriptome Atlas in Natural Environment Reveals Flowering Time Regulatory Network in Alfalfa.","authors":"Yuanyuan Zhang, Haijun Zhao, Haoxin Xiang, Jiashuo Zhang, Lei Wang","doi":"10.1111/pce.15466","DOIUrl":"https://doi.org/10.1111/pce.15466","url":null,"abstract":"Alfalfa (Medicago sativa L.) is a globally cultivated perennial forage legume. Flowering time, an important agronomic trait of alfalfa, is pivotal for farmers to determine the optimal harvest stage, thereby maximizing economic benefits. However, the underlying molecular basis of flowering time regulation in alfalfa remains unclear. Here we generated a comprehensive full-length, seasonal and diurnal transcriptome atlas comprising 108 samples, including two sets of late- and early-flowering alfalfa across spring, summer and autumn in the natural environment. A total of 389 candidate flowering time-related genes were identified in alfalfa, of which 92 were differentially expressed between early and late flowering plants. Further, we revealed that flowering time regulation genes predominantly identified in spring were mainly involved in vernalization, while genes exclusively identified in summer and autumn were primarily involved in circadian and photoperiodic pathways. Moreover, diurnal dynamics of transcriptomes demonstrate the precise orchestration of various biological processes, including chloroplast development, redox processes, biotic stress responses, growth and development, occurs at designated times throughout the day in accordance with external environmental cues. Together, our results provide a valuable resource for future manipulation of genetic control of flowering time in alfalfa, and demonstrate how plants adapt to diurnal and seasonal environments.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sphingobium yanoikuyae 41R9 Enhances Nitrogen Uptake by Modulating Transporter Genes and Root Development in Rapeseed. 油菜籽Sphingobium yanokuyae 41R9通过调节转运基因和根系发育促进氮素吸收

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-03-12 DOI: 10.1111/pce.15471

Youqiang Wang, Donglin Zhao, Zhe Li, Han Zheng, Yiqiang Li, Yanfen Zheng, Cheng-Sheng Zhang

{"title":"Sphingobium yanoikuyae 41R9 Enhances Nitrogen Uptake by Modulating Transporter Genes and Root Development in Rapeseed.","authors":"Youqiang Wang, Donglin Zhao, Zhe Li, Han Zheng, Yiqiang Li, Yanfen Zheng, Cheng-Sheng Zhang","doi":"10.1111/pce.15471","DOIUrl":"https://doi.org/10.1111/pce.15471","url":null,"abstract":"Plant growth-promoting rhizobacteria (PGPR) are widely recognized for enhancing the absorption of mineral nutrients by crops. While Sphingobium species have been reported as PGPRs, their capacity to improve nitrogen use efficiency (NUE) and the underlying regulatory mechanisms are not yet fully understood. Here, a strain 41R9, isolated from the rhizosphere of N-deficient rapeseed, was found to significantly enhance the growth performance of rapeseed under both low and normal N conditions. Genomic analysis revealed that strain 41R9 was closely related to Sphingobium yanoikuyae. 15N isotope tracer experiments confirmed that inoculation with strain 41R9 significantly boosted N uptake and translocation in rapeseed roots. Transcriptome profiling demonstrated that strain 41R9 directly upregulated N transporter genes (NRT2.5 and SLAH1/3), facilitating efficient N acquisition. Furthermore, strain 41R9 maintained jasmonic acid (JA) homoeostasis via JAZ-mediated negative feedback, balancing defense responses and root development, thereby improving the plant's N acquisition capacity in the roots. Metabolomic and in vitro assays further demonstrated that strain 41R9 displayed strong chemotaxis towards kaempferol, a N-deficiency-induced root exudate, suggesting kaempferol might as a chemical effector for S. yanoikuyae recruitment. These findings advance our understanding of PGPR-driven mechanisms in enhancing crop NUE and highlight the potential of harnessing PGPRs for sustainable agriculture.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights Into the Mechanisms of Tonoplast Dicarboxylate Transporter-Induced Plant Tolerance Against Manganese Toxicity in Peach 桃细胞质二羧酸转运体诱导植物抗锰毒性机制的研究。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-03-11 DOI: 10.1111/pce.15468

Kaijie Zhu, Xueke Wang, Jingxian Sun, Iqra Noor, Zezheng Du, Mirza Hasanuzzaman, Chuang Wang, Guohuai Li, Junwei Liu

{"title":"Insights Into the Mechanisms of Tonoplast Dicarboxylate Transporter-Induced Plant Tolerance Against Manganese Toxicity in Peach","authors":"Kaijie Zhu, Xueke Wang, Jingxian Sun, Iqra Noor, Zezheng Du, Mirza Hasanuzzaman, Chuang Wang, Guohuai Li, Junwei Liu","doi":"10.1111/pce.15468","DOIUrl":"10.1111/pce.15468","url":null,"abstract":"<div>\u0000 \u0000 Manganese (Mn) toxicity poses a severe hazard to plant growth, with organic acids playing a crucial role in detoxifying toxic metals. However, the regulatory mechanisms governing the response of organic acids to Mn toxicity remain largely elusive, particularly in perennial fruit crops. Herein, we investigated the physio-biochemical and transcriptomic responses of peach seedlings to Mn toxicity. Organic acids, especially malate, significantly increased in Mn-treated peach seedlings. Subsequently, malate application markedly mitigated Mn toxicity in peach. Further, we identified a key vacuolar malate transporter, PpTDT, whose expression was dramatically induced by both Mn and malate treatments. PpTDT was localised to the vacuolar membrane. Heterologous expression of PpTDT in yeast restored growth arrest and enhanced Mn tolerance. Overexpression of PpTDT in tobacco, peach leaves and roots enhanced Mn toxicity tolerance, and increased malate and Mn content. Conversely, silencing of PpTDT in peach seedlings exacerbated Mn toxicity, resulting in decreased malate and Mn content. These findings unveil the role of PpTDT in facilitating intracellular chelation of Mn through malate transport, thereby imparting Mn toxicity tolerance in peach. Our study also highlights the potential of malate as an natural compound for improving Mn toxicity tolerance in peach and potentially other fruit crops.</div>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":"48 6","pages":"4703-4718"},"PeriodicalIF":6.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Size Matters: Influence of Available Soil Volume on the Root Architecture and Plant Response at Transcriptomic and Metabolomic Levels in Barley 大小问题：有效土壤体积对大麦根构型和植株转录组学和代谢组学响应的影响。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-03-10 DOI: 10.1111/pce.15457

Fabiano Sillo, Sebastian R. G. A. Blaser, Carla Díaz-Tielas, Jessica Clayton, Fabrizio Araniti, Adela M. Sánchez-Moreiras, Timothy S. George, Raffaella Balestrini, Doris Vetterlein

{"title":"Size Matters: Influence of Available Soil Volume on the Root Architecture and Plant Response at Transcriptomic and Metabolomic Levels in Barley","authors":"Fabiano Sillo, Sebastian R. G. A. Blaser, Carla Díaz-Tielas, Jessica Clayton, Fabrizio Araniti, Adela M. Sánchez-Moreiras, Timothy S. George, Raffaella Balestrini, Doris Vetterlein","doi":"10.1111/pce.15457","DOIUrl":"10.1111/pce.15457","url":null,"abstract":"Pot size is a critical factor in plant growth experiments, influencing root architecture, nutrient uptake, and overall plant development as well as sensing of stress. In controlled environments, variation in pot size can impact phenotypic and molecular outcomes and may bias experimental results. Here, we investigated how pot size affects the root system architecture and molecular responses of two barley genotypes, the landrace BERE and the modern elite CONCERTO, through assessment of shoot and root traits and by using X-ray computed tomography complemented by transcriptomic and metabolomic analyses. The two genotypes showed distinctly different adaptations to changes in pot size. The landrace showed greater stability and adaptability with consistent root traits and enhanced accumulation of osmoprotectant metabolites across different pot sizes with respect to CONCERTO. Conversely, the elite line was more sensitive to pot size variations, particularly showing altered root architecture and transcriptomic responses. Overall, this study highlights the importance of selecting an appropriate pot size for plant growth experiments, particularly when focused on root traits, and highlights the importance of considering the physiological and molecular changes due to growth environment choice in experimental design in barley.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":"48 6","pages":"4685-4702"},"PeriodicalIF":6.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pce.15457","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outside Front Cover Image 外封面图像

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-03-10 DOI: 10.1111/pce.15480

Jialu Wei, Tingting Guo, Qi Mu, Boris M.E. Alladassi, Ravi V. Mural, Richard E. Boyles, Leo Hoffmann Jr., Chad M. Hayes, Brandi Sigmon, Addie M. Thompson, Maria G. Salas-Fernandez, William L. Rooney, Stephen Kresovich, James C. Schnable, Xianran Li, Jianming Yu

引用次数: 0

Stomata-Photosynthesis Synergy Mediates Combined Heat and Salt Stress Tolerance in Sugarcane Mutant M4209 气孔-光合成协同作用介导甘蔗突变体 M4209 的耐热和耐盐综合胁迫能力

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-03-07 DOI: 10.1111/pce.15424

Pooja Negi, Manish Pandey, Radha K. Paladi, Arnab Majumdar, Shailaja P. Pandey, Vitthal T. Barvkar, Rachayya Devarumath, Ashish K. Srivastava

{"title":"Stomata-Photosynthesis Synergy Mediates Combined Heat and Salt Stress Tolerance in Sugarcane Mutant M4209","authors":"Pooja Negi, Manish Pandey, Radha K. Paladi, Arnab Majumdar, Shailaja P. Pandey, Vitthal T. Barvkar, Rachayya Devarumath, Ashish K. Srivastava","doi":"10.1111/pce.15424","DOIUrl":"10.1111/pce.15424","url":null,"abstract":"Sugarcane (Saccharum officinarum L.) is an economically important long-duration crop which is currently facing concurrent heat waves and soil salinity. The present study evaluates an inducible salt-tolerant sugarcane mutant M4209, developed via radiation-induced mutagenesis of elite check variety Co 86032, under heat (42/30°C; day/night), NaCl (200 mM) or heat + NaCl (HS)-stress conditions. Though heat application significantly improved plant growth and biomass in both genotypes, this beneficial impact was partially diminished in Co 86032 under HS-stress conditions, coinciding with higher Na+ accumulation and lower triacylglycerol levels. Besides, heat broadly equalised the negative impact on NaCl stress in terms of various physiological and biochemical attributes in both the genotypes, indicating its spaciotemporal advantage. The simultaneous up- and downregulation of antagonistic regulators, epidermal patterning factor (EPF) 9 (SoEPF9) and SoEPF2, respectively attributed to the OSD (Open Small Dense) stomatal phenotype in M4209, which resulted into enhanced conductance, transpirational cooling and gaseous influx. This led to improved photoassimilation, which was supported by higher plastidic:nonplastidic lipid ratio, upregulation of SoRCA (Rubisco activase) and better source strength, resulting in overall plant growth enhancement across all the tested stress scenarios. Taken together, the present study emphasised the knowledge-driven harnessing of stomatal-photosynthetic synergy for ensuring global sugarcane productivity, especially under “salt-heat” coupled stress scenarios.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":"48 6","pages":"4668-4684"},"PeriodicalIF":6.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pce.15424","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Function of PpKCS6 in Regulating Cuticular Wax Synthesis and Drought Resistance of Kentucky Bluegrass PpKCS6在调控肯塔基蓝草表皮蜡合成及抗旱性中的作用

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-03-06 DOI: 10.1111/pce.15465

Jia Jiang, Xueling Zheng, Tiantian He, Xiashun Liu, Qianhan Zhao, Wei Tan, Liangbing Xiong, Bing Li, Hang Yin, Gyimah Daniel Agyei, Fuchun Xie, Guowen Cui, Yajun Chen

{"title":"The Function of PpKCS6 in Regulating Cuticular Wax Synthesis and Drought Resistance of Kentucky Bluegrass","authors":"Jia Jiang, Xueling Zheng, Tiantian He, Xiashun Liu, Qianhan Zhao, Wei Tan, Liangbing Xiong, Bing Li, Hang Yin, Gyimah Daniel Agyei, Fuchun Xie, Guowen Cui, Yajun Chen","doi":"10.1111/pce.15465","DOIUrl":"10.1111/pce.15465","url":null,"abstract":"<div>\u0000 \u0000 Drought stress significantly limits plant growth and crop productivity. Cuticular wax minimizes plant water loss and contributes to drought resistance. Kentucky bluegrass (Poa pratensis L.) is a widely used cool-season turfgrass worldwide. However, the molecular mechanisms underlying the regulation of dynamic changes in cuticular wax in relation to drought resistance in Kentucky bluegrass remain unclear. Here, we compared molecular mechanisms of cuticular wax biosynthesis in two Kentucky bluegrass cultivars ‘Maoershan’ (drought-tolerant) and ‘Brilliant’ (drought-sensitive). The results showed that ‘Brilliant’ with lower wax content, suffered more severe morphological and physiological damage from drought stress than the ‘Maoershan’. Through transcriptome analysis of these two cultivars, a hub gene of PpKCS6, involved in cuticular wax synthesis, was identified. Overexpression of PpKCS6 promoted the synthesis of very long-chain fatty acids, especially increased the content of fatty acids and alkanes with carbon chains above C24. This led to greater cuticular wax accumulation, which further protected the plants against water loss and improved photosynthesis and water use efficiency. Alternatively, RNAi-PpKCS6 lines exhibited the opposite performance to the overexpression line. These results demonstrate that PpKCS6 plays an important role in drought stress resistance by regulating wax biosynthesis.</div>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":"48 6","pages":"4643-4655"},"PeriodicalIF":6.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Root Phenotypes for Compacted Soils: Enhancing Root-Soil-Microbe Interactions 优化压实土壤的根表型：增强根-土壤-微生物的相互作用。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-03-06 DOI: 10.1111/pce.15462

Jingqi Xu, Zijian Long, Baoru Sun, Fangbo Zhang, Jianbo Shen, Kemo Jin

{"title":"Optimizing Root Phenotypes for Compacted Soils: Enhancing Root-Soil-Microbe Interactions","authors":"Jingqi Xu, Zijian Long, Baoru Sun, Fangbo Zhang, Jianbo Shen, Kemo Jin","doi":"10.1111/pce.15462","DOIUrl":"10.1111/pce.15462","url":null,"abstract":"<div>\u0000 \u0000 Soil compaction impedes root growth, reduces crop yields, and threatens global food security and sustainable agriculture. Addressing this challenge requires a comprehensive understanding of root-soil interactions in compacted environments. This review examines key root traits—architectural, anatomical, biochemical, and biomechanical—that enhance plant resilience in compacted soils. We discuss how these traits influence root penetration and the formation of more favorable soil pore structures, which are crucial for alleviating compaction stress. Additionally, we explore the molecular mechanisms underlying root adaptation, identifying key genetic and biochemical factors that contribute to stress-tolerant root phenotypes. The review emphasizes the role of root-microbe interactions in boosting root adaptability under compaction. By integrating these insights, we propose a framework for breeding crops with resilient root systems that thrive in high soil strength, supporting sustainable agricultural practices essential for food security amidst environmental challenges.</div>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":"48 6","pages":"4656-4667"},"PeriodicalIF":6.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial Regulation of Rice Leaf Morphology by miRNA-Target Complexes During Viral Infection 病毒侵染过程中miRNA-Target复合物对水稻叶片形态的空间调控

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-03-05 DOI: 10.1111/pce.15460

Lu Wang, Yuansheng Wu, Jialin Zhang, Shanshan Li, Junjie Ren, Liyuan Yang, Wenyang Ye, Xinrong Ying, Jiajun Liu, Xinzhou Liu, M. S. Salem, Chengqiang Ding, Jianguo Shen, Zujian Wu, Jianguo Wu, Shanshan Zhao

{"title":"Spatial Regulation of Rice Leaf Morphology by miRNA-Target Complexes During Viral Infection","authors":"Lu Wang, Yuansheng Wu, Jialin Zhang, Shanshan Li, Junjie Ren, Liyuan Yang, Wenyang Ye, Xinrong Ying, Jiajun Liu, Xinzhou Liu, M. S. Salem, Chengqiang Ding, Jianguo Shen, Zujian Wu, Jianguo Wu, Shanshan Zhao","doi":"10.1111/pce.15460","DOIUrl":"10.1111/pce.15460","url":null,"abstract":"Leaf morphogenesis is essential for plant growth and development, yet the mechanisms by which plant viruses induce changes in leaf shape are not well understood. Rice ragged stunt virus (RRSV) infection induces distinct morphological abnormalities in rice leaves, including leaf tip curling and serrated margins, through unknown pathogenic mechanisms. This study reveals that key regulatory microRNAs (miR164, miR319 and miR156) and their target genes (CUC, TCP and SPL) exhibit entirely opposite expression patterns in healthy and RRSV-infected leaves, indicating a profound impact on the leaf morphogenesis network. Significantly, the core protein OsCUC1, which typically functions by forming dimers, shows abnormal expression in the peripheral zone of the shoot apical meristem under viral infection, leading to disruptions in leaf development. OsTCP1 was found to dynamically regulate OsCUC1 dimer formation by modifying its subcellular localization and interacting with OsSPL14 and OsSPL17, thereby influencing their regulatory functions. Genetic disruptions of OsCUC1, OsTCP1 and OsSPL14/OsSPL17 enhance the severity of RRSV infection, demonstrating their critical involvement in the viral pathogenic strategy. The research uncovers a novel mechanism by which RRSV manipulates the expression and interactions of key regulatory factors, disrupting the delicate balance of the leaf morphogenesis network. These findings expand our understanding of viral manipulation of host development and provide a foundation for innovative strategies to enhance crop resilience.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":"48 6","pages":"4625-4642"},"PeriodicalIF":6.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0