Plant, Cell & Environment最新文献

{"title":"Ecodormancy or Endodormancy? PtTFL2 as the Key Switch in Regulating Dormancy Transitions in Pinus tabuliformis.","authors":"Yi Li, Zijian Hu","doi":"10.1111/pce.15587","DOIUrl":"https://doi.org/10.1111/pce.15587","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combating Parasitic Weeds by Manipulation of Strigolactones Efflux Transporter.","authors":"Meicheng Zhao, Xianmin Diao","doi":"10.1111/pce.15577","DOIUrl":"https://doi.org/10.1111/pce.15577","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Functional Characterization of Soybean Microexon in Response to Saline-Alkali Stress.","authors":"Yang Li, Qingxi Fang, Yingxue Cao, Mingyu Yang, Jing Wang, Meizi Wang, Na Li, Fanli Meng","doi":"10.1111/pce.15596","DOIUrl":"https://doi.org/10.1111/pce.15596","url":null,"abstract":"<p><p>Salt-alkali stress is one of the most widespread and devastating abiotic stress. Alternative splicing is a response pathway to such stress. However, the role of microexons in response to salt-alkali stress in soybean remains obscure. In this study, we identified microexons related to salt-alkali stress. We focused on analyzing the conserved sequence patterns of 27-30 bp microexons, and consistently observed conserved GT and AG sequences at the 5' and 3' ends of these microexons. Additionally, we found that the AP2 protein domain had the most abundant microexons. Interestingly, the majority of microexons in the AP2 transcription factor were 9 bp in length, encoding a conserved valine (V), tyrosine (Y), or leucine (L), suggesting their indispensable role. Furthermore, we cloned two transcripts of three AP2 genes with and without the salt-alkali stress-induced microexon and generated stable transgenic soybeans. Surprisingly, we discovered that the depletion of microexons in the AP2 gene enhances salt-alkali resistance. Collectively, this characterization of microexon suggests a new scenario explaining soybean salt-alkali stress resistance.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Propionic Acid Signalling Modulates Stomatal Opening and Drives Energy Metabolism to Enhance Drought Resistance in Wheat (Triticum aestivum L.).","authors":"Zongzhen Li, Chenxi Li, Pengbin Han, Yihan Wang, Yongzhe Ren, Zeyu Xin, Tongbao Lin, Yanhao Lian, Zhiqiang Wang","doi":"10.1111/pce.15589","DOIUrl":"https://doi.org/10.1111/pce.15589","url":null,"abstract":"<p><p>Drought stress caused by global climate change severely imperils crop productivity and increases environmental deterioration. Wheat (Triticum aestivum L.) is an important worldwide food crop. Drought resistance in wheat encompasses functional gene transcription, metabolism, hormone signalling, and protein modifications. However, the underlying mechanisms by which these regulatory responses are coordinated remain unknown. Herein, we report a drought-resistance network in which wheat triggers a dynamic metabolic flux conversion from propionic acid (PA) to the tricarboxylic acid (TCA) cycle through beta-oxidation of fatty acids and stimulates crosstalk of various hormonal signals. It is also possible that P300/CREB regulates histone acetylation to confer drought resistance in wheat. Exogenous PA drives the TCA cycle and glycolysis and promotes stomatal closure through hormones crosstalk. From Aegilops tauschii Cosson (the diploid progenitor of common wheat) to wheat, this novel PA function serves as a survival strategy against environmental changes, and was validated in wheat field experiments. Our results highlight a new survival strategy that triggers the comprehensive and systemic effects of functional genes, metabolomics, hormone signalling, and protein modification on drought resistance to provide novel insights into improving the agroecological environment.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichoderma afroharzianum T22 Induces Rhizobia and Flavonoid-Driven Symbiosis to Promote Tolerance to Alkaline Stress in Garden Pea.","authors":"Asha Thapa, Md Rokibul Hasan, Ahmad H Kabir","doi":"10.1111/pce.15581","DOIUrl":"https://doi.org/10.1111/pce.15581","url":null,"abstract":"<p><p>Soil alkalinity is a limiting factor for crops, yet the role of beneficial fungi in mitigating this abiotic stress in garden pea is understudied. In this study, Trichoderma afroharzianum T22 colonised the roots of garden pea cultivars exposed to soil alkalinity in a host-specific manner. In alkaline-exposed Sugar Snap, T22 improved growth parameters, consistent with increased tissue mineral content, particularly Fe and Mn, as well as enhanced rhizosphere siderophore levels. The split-root assay demonstrated that the beneficial effects of T22 on alkaline stress mitigation are the result of a whole-plant association rather than localised root-specific effects. RNA-seq analysis showed 575 and 818 differentially expressed genes upregulated and downregulated in the roots inoculated with T22 under alkaline conditions. The upregulated genes were mostly involved in the flavonoid biosynthetic pathway (monooxygenase activity, ammonia-lyase activity, 4-coumarate-CoA ligase), along with genes related to mineral transport and redox homoeostasis. Further, a flavonoid precursor restored plant health even in the absence of T22, confirming the role of microbial symbiosis in mitigating alkaline stress. Interestingly, T22 restored the abundance of rhizobia, particularly Rhizobium leguminosarum and Rhizobium indicum, along with the induction of NifA, NifD, and NifH in nodules, suggesting a connection between T22 and rhizobia under soil alkalinity. Further, the elevated rhizosphere siderophore, root flavonoid, expression of PsCoA (4-coumarate-CoA ligase) as well as the relative abundance of TaAOX1 and R. leguminosarum diminished when T22 was substituted with exogenous Fe. This suggests that exogenous Fe eliminates the need for microbiome-driven mineral mobilisation, while T22-mediated alkaline stress mitigation depends on flavonoid-driven symbiosis and R. leguminosarum abundance. It was further supported by the positive interaction of T22 on R. leguminosarum growth in alkaline media. Thus, the beneficial effect of T22 on rhizobia likely stems from their interactions, not solely from the improved mineral status, particularly Fe, in plants. This study provides the first mechanistic insights into T22 interactions with host and rhizobia, advancing microbiome strategies to alleviate soil alkalinity in peas and other legumes.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-Mediated SiEPF2 Mutagenesis Attenuates Drought Tolerance and Yield in Foxtail Millet (Setaria italica).","authors":"Jian-Hong Hao, Xueting Kang, Lingqian Zhang, Jiayi Chen, Dan Wang, Shuqi Dong, Xiaorui Li, Lulu Gao, Guanghui Yang, Xiangyang Yuan, Xiaoqian Chu, Jia-Gang Wang","doi":"10.1111/pce.15597","DOIUrl":"https://doi.org/10.1111/pce.15597","url":null,"abstract":"<p><p>Plants employ peptide ligands to coordinate development and integrate environmental signals via dedicated cascades (#ref-0013). Epidermal patterning factor (EPF), plays a significant role in regulating stomatal density, seed germination and panicle development (#ref-0008). EPF/EPFL enhance drought tolerance by reducing stomatal density have been reported in multiple species, including Hordeum vulgare, Arabidopsis thaliana, Populus spp., Vitis vinifera, Sorghum bicolor and Brassica napus (#ref-0001). Our previous study has shown that EPF can mediate drought resistance in foxtail millet by regulating stomatal density (#ref-0004). Furthermore, evidence suggests that plants can regulate photosynthesis through stomatal modification, ultimately enhancing yield (#ref-0005). Beyond stomatal density regulation, the EPF/EPFL gene family modulates seed germination through phytohormone signalling and regulates inflorescence development via ligand-receptor interactions (#ref-0007). OsEPFLs act as upstream ligands for the OsER1 receptor, activating the MAPK signalling cascade to regulate panicle morphogenesis (#ref-0002). In this study, we sought to elucidate how SiEPF2 balances drought resistance and yield in foxtail millet by modulating stomatal density and panicle morphology. Our findings not only provide novel insights into SiEPF2's role in abiotic stress responses but also contribute valuable genetic resources for high-yield breeding programmes in millet crops.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recycling of Trans-Golgi SNAREs Promotes Apoplastic Effector Secretion for Effective Host Invasion in Magnaporthe oryzae.","authors":"Lili Lin, Qiuqiu Wu, Shuang Wang, Qing Gong, Xiuwei Huang, Yakubu Saddeeq Abubakar, Yue Liu, Jiaying Cao, Jiexiong Hu, Zonghua Wang, Guodong Lu, Wenhui Zheng","doi":"10.1111/pce.15582","DOIUrl":"https://doi.org/10.1111/pce.15582","url":null,"abstract":"<p><p>Vesicle transport is crucial for pathogenic fungi, but the mechanisms that control the secretion of effector proteins are not yet fully understood. Here, we have uncovered a novel pathway in which retromer and trans-Golgi (TGN) SNARE proteins co-regulate the proper secretion of apoplastic effectors in Magnaporthe oryzae. It was found that a TGN-associated SNARE complex, consisting of MoSnc1, MoTlg1, MoTlg2 and MoVti1, is critical for growth, development and pathogenicity in the fungus. In addition, the TGN-associated SNARE complex is indispensable for the proper secretion of apoplastic effectors. Furthermore, we found that the dynamin-like protein MoVps1, an upstream regulator of the retromer complex, regulates the fission of MoVps35-coated vesicles and the proper localisation of the TGN-associated SNARE complex. Additionally, treatment with perphenazine, a potent dynamin inhibitor, perturbs the fungal developmental similar to MoVPS1 disruption, highlighting the central regulatory role of dynamin in M. oryzae and suggesting the potential efficacy the control and management of the rice blast. Taken together, the study uncovered a specific mechanism by which MoVps1 and the retromer complex co-regulate the positioning of TGN-associated SNARE proteins to effectively promote effector secretion. This study widens our horizon on the mechanism of effector secretion in phytopathogenic fungi and underscores the importance of vesicle transport in fungal pathogenesis.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benzoic Acid as a Key Signalling Molecule in Establishing Nitrogen-Fixing Microbiota in Cereal Crops: Potential Mechanisms and Strategies for Enhancing Associative Nitrogen Fixation.","authors":"Junhao Shi, Ruirui Li, Yongdong Yu, Hong Liao, Yongjia Zhong","doi":"10.1111/pce.15588","DOIUrl":"https://doi.org/10.1111/pce.15588","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic Genomics: A Powerful Tool for Unlocking New Possibilities in Plant Biology.","authors":"Zifeng Yang, Nan Wang, Yuling Jiao, Qiao Zhao","doi":"10.1111/pce.15590","DOIUrl":"https://doi.org/10.1111/pce.15590","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptation in Wood Anatomical Traits to Temperature and Precipitation-A Common Garden Study.","authors":"Tiantian Pan, Travis G Britton, Julian Schrader, Emma Sumner, Dean Nicolle, Brendan Choat, Ian J Wright","doi":"10.1111/pce.15576","DOIUrl":"https://doi.org/10.1111/pce.15576","url":null,"abstract":"<p><p>Indisputably, temperature and precipitation are key environmental variables driving plant trait variation and shaping plant ecological strategies. However, it is challenging to ascertain their relative influences because site temperature and precipitation are often correlated. Here, using Eucalyptus as a model system representing woody evergreen species more broadly, we sought to disentangle their influence on wood anatomical traits underpinning plant hydraulics. From a common garden we sampled 29 pairs of closely-related Eucalyptus species, each species-pair representing either a contrast in site temperature or precipitation, but never both. Very clearly, and both in phylogenetic and non-phylogenetic analyses, species from lower-rainfall and from colder regions had thicker vessel walls, likely an adaptation to drought and freezing, enabling water transport at more negative water potentials with reduced risk of cavitation or vessel implosion. On average, species from warmer regions had smaller vessels, but theoretical hydraulic conductivity remained stable across site temperatures due to increased vessel density compensating for reduced diameters. These trends being observed for adult plants grown under common conditions suggests that key hydraulic anatomy traits are \"hard-wired\", and gene × environment interactions are relatively weak. This is a key insight for understanding the trait-basis of plant ecological strategies related to site climate.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}