Plant, Cell & Environment最新文献

{"title":"Heat Shock Transcription Factors as Integrative Hubs for Plant Stress Adaptation: Decoding Regulatory Networks Toward Climate-Resilient Crop Design.","authors":"Yunxuan Feng, Pengguo Xia","doi":"10.1111/pce.70185","DOIUrl":"https://doi.org/10.1111/pce.70185","url":null,"abstract":"<p><p>As sessile organisms, plants deploy heat shock transcription factors (HSFs) as key coordinators within a broader transcriptional network that includes bZIPs, MYBs, NACs, and DREBs to combat abiotic/biotic stresses under climate change. Beyond activating heat shock proteins and antioxidant systems, HSFs maintain redox homoeostasis by orchestrating hormone pathways and ROS-hormone signalling crosstalk, and enhance cross-kingdom defence through secondary metabolite synthesis, thus extending their function from thermotolerance to drought, salinity, and pathogen defence. However, current research is limited by overreliance on model plants, unclear HSF dynamics under coupled stresses, and unresolved epigenetic regulation of stress memory. Furthermore, CRISPR editing faces challenges with multigene coordination and field validation. This review integrates HSF structural evolution and network mechanisms, proposing innovative strategies: cross-species genomics, computational modelling of HSF networks, CRISPR-based synthetic stress circuits, and targeted epigenetic modifications for transgenerational resilience. These approaches aim to elucidate HSF-mediated epigenetic stress memory, bridging molecular research with stress-resilient crop breeding to provide a blueprint for next-generation climate-smart crops and sustainable solutions for global food security.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycerophosphodiester Phosphodiesterase ZmGPX-PDE1 Regulates Phosphorus Remobilisation and JA-Mediated Anther Development in Maize.","authors":"Yan Sun, Chenxi Fu, Zikai Xu, Jinting Zhang, Yang Han, Siji Wang, Manli Zhao, Jianbo Shen, Lingyun Cheng","doi":"10.1111/pce.70189","DOIUrl":"https://doi.org/10.1111/pce.70189","url":null,"abstract":"<p><p>Phosphorus (P) is essential for plant growth, yet its limited availability necessitates efficient utilisation, especially during reproductive stages where P remobilisation mechanisms are not fully understood. This study investigates the role of the maize gene ZmGPX-PDE1, which encodes a glycerophosphodiester phosphodiesterase involved in phospholipid metabolism by hydrolysing glycerophosphodiesters to glycerol-3-phosphate, regulates P remobilisation and reproductive development. Using loss-of-function mutants and overexpression lines, we demonstrated that ZmGPX-PDE1 facilitates the redistribution of P from vegetative tissues to reproductive organs, thereby enhancing biomass accumulation, grain yield, and pollen viability under low-P conditions. Furthermore, our results reveal that ZmGPX-PDE1 influences jasmonic acid (JA) signalling, a critical pathway for anther dehiscence and male fertility. Loss of ZmGPX-PDE1 function results in impaired P remobilisation, reduced anther P content, and disrupted JA signalling, leading to decreased pollen quality and yield. These findings provide new insights into the molecular mechanisms underlying P homeostasis in maize and highlight ZmGPX-PDE1 as a promising target for developing P-efficient cultivars suitable for sustainable agriculture.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tree Saplings Readily Take Up NO₂ During Foliar ¹⁵NO₂ Fumigation.","authors":"Meng Yao, Ronghua Kang, Erik A Hobbie, Qing-Wei Wang, Lei Duan, Jan Mulder, Yuqi Liu, Jin Li, Jingran Ma, Chao Wang, Yunting Fang","doi":"10.1111/pce.70184","DOIUrl":"https://doi.org/10.1111/pce.70184","url":null,"abstract":"<p><p>Forest canopy nitrogen dioxide (NO₂) uptake is recognised as a potentially important N input. However, how N addition influences foliar NO₂ uptake and allocation of assimilated NO₂ in different tree organs has been poorly quantified. We conducted a ¹⁵NO₂ fumigation experiment using 3-year-old saplings of Fraxinus mandshurica, Pinus koraiensis, Quercus mongolica (Q. mongolica) and Larix gmelinii (L. gmelinii) and measured assimilation into different tree organs. Total ¹⁵N recovery ranged from 9% to 74% in the light and varied with species. With soil N addition, ¹⁵N recovery increased in Q. mongolica but decreased in L. gmelinii, which we attributed to opposite responses of stomatal density and leaf area between these two species to soil N addition. These indicate that foliar NO₂ uptake amounts are likely associated with tree N demand. Leaves were the dominant sink for N derived from ¹⁵NO₂ and accounted for 60% to 97% of total recovery, suggesting that most foliar-assimilated NO₂ is initially stored in leaves. Our study indicates that tree canopies could assimilate 0.51 ± 0.07 kg N ha^-1 y^-1 atmospheric NO₂ in temperate forests, which provides references for model large-scale modelled estimates of canopy NO₂ uptake. These data improve the understanding of N cycling between atmosphere and forest ecosystems.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of Tempranillo-Like Proteins Promotes Dormancy Release in Poplar.","authors":"Daniela Gómez-Soto, Paolo Maria Triozzi, Daniel Conde, Cristina Del Barrio, Isabel Allona, Mariano Perales","doi":"10.1111/pce.70182","DOIUrl":"https://doi.org/10.1111/pce.70182","url":null,"abstract":"<p><p>Trees in temperate and boreal latitudes synchronize their growth-dormancy cycles with seasonal environmental variations to ensure their survival over the years. Dormancy control is crucial during winter when plants cease growth and establish buds to protect their apical meristems from cold temperatures. To overcome endormancy, initiate bud break, and restore growth, plants must be exposed to a specific duration of chilling, referred to as the chilling requirement, which is species- and ecotype-dependent. In this study, we study the novel roles of two TEMPRANILLO-like genes (TEML1 and TEML2) in the annual cycle of poplar. We demonstrated that Populus TEML genes are regulated by photoperiod, cold temperatures and the circadian clock, and they are induced in buds by short days and chilling treatment. Notably, their function diverges from the role of its Arabidopsis ortholog AtTEM, which regulates FLOWERING LOCUS T (FT) transcription and the photoperiodic flowering transcription. Transcriptomic analysis of apical buds during short days and chilling treatment revealed that the overexpression of TEML1 and TEML2 accelerate dormancy release by modulating the expression of dormancy regulators and growth-promoting genes.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive Diversity Effect on Woody Biomass Production by Promoting Cell Number and Cell Wall Thickness.","authors":"Hong-Tu Zhang, Tongyan Liu, Shan Li, Bernhard Schmid, Helge Bruelheide, Keping Ma, Zhiyao Tang","doi":"10.1111/pce.70188","DOIUrl":"https://doi.org/10.1111/pce.70188","url":null,"abstract":"<p><p>While biodiversity has been widely shown to promote tree growth in forests, the effects of diversity at the cellular level remain unclear. This hinders a deeper understanding of how diversity influences wood production. Here, we investigated the influence of neighbourhood composition on wood formation processes at the cellular level. We found that neighbourhood diversity significantly increased both the number and wall thickness of xylem fibre cells generated during the growing season, resulting in enhanced biomass growth. Compared with monocultures, woody biomass production at the highest neighbourhood species richness (NSR = 4) increased by an average of 19%-29% across the studied species. Neighbourhood diversity also led to a shift in cambium phenology. An earlier start of cambium activity and an extended duration of wall thickening were observed under higher tree species richness. Furthermore, neighbourhood competition decreased vessel area, which was closely associated with cell wall thickness. Our study offers a microscopic perspective on the effects of diversity and competition on wood formation and woody biomass production. We highlight that the contribution of diversity to woody biomass accumulation may be underestimated when variation in cell wall thickness is ignored.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Mysterious Mechanism of Aquaporin-Mediated CO₂ Transport.","authors":"Hui Wang, Huayan Zhao","doi":"10.1111/pce.70187","DOIUrl":"https://doi.org/10.1111/pce.70187","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation.","authors":"Weiwei Liu, Qiuyue Ou, Meichang Feng, Yue Li, Linghan Huang, Xuan Peng, Xiaoqiang Zhong, Guoqiang Huang, Hengbo Wang, Jinlong Guo","doi":"10.1111/pce.70180","DOIUrl":"https://doi.org/10.1111/pce.70180","url":null,"abstract":"<p><p>Cold stress is a critical environmental factor adversely affecting plant growth and development. As a tropical-origin crop constituting the primary global source of sucrose, sugarcane (Saccharum spp. hybrid) exhibits particular vulnerability to suboptimal temperature conditions, with chilling injury substantially compromising its yield potential. Despite its agricultural significance, the molecular mechanisms underlying cold acclimation in sugarcane remain poorly characterized. Here, we report a cold-repressed 1R-MYB gene, ScMYB7, from sugarcane, whose promoter (pro-ScMYB7) contains multiple cis-acting elements, including two cytosine-phosphate diester-guanine (CpG) islands. Bisulfite sequencing PCR (BSP) and qPCR results showed that low-temperature treatment increased the methylation level of the CpG islands in the promoter to reduce the transcription of the ScMYB7 gene. The outcomes of GUS enzyme activity measurement of the promoter also indicated that low-temperature treatment inhibits the promoter's transcriptional activity, and methylation inhibitors could alleviate this inhibition. By generating transgenic Arabidopsis lines overexpressing ScMYB7, ScMYB7's roles in regulating cold tolerance were investigated. We observed that the transgenic plants reduced cold tolerance, featured by a decreased survival rate after recovery, fluctuated physiological traits, and significantly lower expression levels of the C-repeat binding factor (CBF)-dependent pathway genes (AtCBFs, AtCOR15, and AtRD29A). Yeast one-hybrid assays demonstrated direct binding of ScMYB7 to the AtCBF1 promoter, while repression of sugarcane ScDREB1A occurred indirectly. Furthermore, the dual-luciferase reporter assay indicated that ScMYB7 was able to inhibit the expression of the AtCBF1 or ScDREB1A. Taken together, we propose a model in which ScMYB7 acts as a repressor of cold tolerance via the CBF-dependent pathway. Under low-temperature stress, increased methylation of the pro-ScMYB7 promoter reduces ScMYB7 expression, thereby alleviating its repression of sugarcane DREB/CBF-type transcription factors and enhancing cold adaptation.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"S-Methyl-l-Cysteine Sulfoxide: A Hidden Layer of Defences Against Herbivorous Insects in Brassicaceae.","authors":"Laura Bellec, Célia Le Maire, Nathalie Marnet, Chrystelle Paty, Anne-Marie Cortesero, Maxime R Hervé","doi":"10.1111/pce.70179","DOIUrl":"https://doi.org/10.1111/pce.70179","url":null,"abstract":"<p><p>Plants defend themselves against herbivorous insects through diverse morphological and biochemical traits. Non-protein amino acids (NPAAs) are an important component of the plant metabolome, although their defensive function remains largely unexplored. Here, we investigated the role of S-methyl-l-cysteine sulfoxide (SMCSO), a sulphur-containing NPAA accumulated in Brassicaceae, in mediating plant defence against herbivorous insects. SMCSO was quantified in inflorescences, leaves (young and old) and roots (primary and secondary) of 14 Brassicaceae species. Additionally, feeding tests on artificial substrates supplemented with physiological SMCSO levels were conducted with both generalist and specialist herbivorous insects feeding on one of the different plant parts studied. In line with the optimal defence theory, we found higher SMCSO levels in reproductive than vegetative tissues, and in young leaves and primary roots compared to old leaves and secondary roots, respectively. SMCSO also exerted a consistent phagodeterrent effect on generalist herbivores, while specialists showed variable responses from deterrence to stimulation. This study provides the first evidence of the influence of this compound on herbivore feeding behaviour, with effects depending on the diet breadth. These findings broaden our understanding of plant chemical defence complexity and highlight the ecological role of NPAAs in plant-insect interactions.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Transcription Factor MYB8 Positively Regulates Flavonoid Biosynthesis of Scutellaria baicalensis in Response to Drought Stress.","authors":"Chong Chen, Xiaofang Zhou, Bo Cao, Shan Feng, Tiantian Bin, Yali Zhang, Pufan Gao, Yumeng Lu, Xian Li, Lianjin Liu, Suying Hu, Bowen Zheng, Guishuang Li, Chengke Bai","doi":"10.1111/pce.70178","DOIUrl":"https://doi.org/10.1111/pce.70178","url":null,"abstract":"<p><p>Drought stress dynamically reprograms specialised metabolism in medicinal plants. However, the transcriptional regulatory modules governing stress-adaptive metabolite synthesis remain poorly characterised. Here, we identified SbMYB8 as a drought-responsive transcription factor showing nuclear localisation and dose-dependent induction under drought in Scutellaria baicalensis. SbMYB8 activation triggered coordinated upregulation of six baicalin biosynthetic genes, elevating total baicalin and aglycones. Heterologous overexpression in Arabidopsis thaliana revealed SbMYB8's conserved regulatory function, driving anthocyanin accumulation (2.3-fold), flavonoid hyperproduction (5.8-fold), and developmental plasticity through enhanced lateral root proliferation. Specifically, we established the first stable S. baicalensis genetic transformation system, enabling tissue-specific dissection of SbMYB8 function. Transgenic OE-SbMYB8 lines exhibited root architectural remodelling (thickened primary roots, increased lateral root density) and root-specific flavonoid amplification (baicalin 1.8-fold; total flavonoids 3.5-fold), coupled with hierarchical induction of 12 pathway genes. Low-dose PEG (2.5%) synergised with SbMYB8 to transiently boost aglycone synthesis, whereas high-dose stress (5%) disrupted this coordination, suppressing biosynthetic machinery and metabolite yields. Mechanistically, yeast one-hybrid and dual-luciferase assays revealed SbMYB8 directly binds cis-elements in target promoters to orchestrate pathway activation. Based on the above results, we propose a SbMYB8-mediated \"drought perception - transcriptional activation - metabolic response\" network and provide transformative tools for precision breeding of stress-resilient medicinal plants.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Regulation Differences of VRS Genes in the Development of Lateral Spikelets in Two-Rowed Barley.","authors":"Liping Shen, Zhiwen Sun, Yangyang Liu, Kuocheng Shen, Zhimin Wang, Botao Ye, Ziying Wang, Zifeng Guo","doi":"10.1111/pce.70183","DOIUrl":"https://doi.org/10.1111/pce.70183","url":null,"abstract":"<p><p>The barley (Hordeum vulgare L.) spike consists of one central and two lateral spikelets at each rachis node. In two-rowed barley, only the central spikelet is fertile, the lateral spikelets also produce grain while in six-rowed barley. Five SIX-ROWED SPIKE genes (VRS1-5) have been identified as regulators of lateral spikelet fertility in barley, but the underlying genetic mechanisms of these VRS genes remain unclear. In this study, we conducted a detailed observation of the development process of the lateral spikelets in two-rowed barley and performed comparative transcriptome analysis to investigate gene expression differences between vrs1-5 mutants and wild-type spikelets. This revealed the differences in the downstream pathways regulated by the VRS genes in lateral spikelet development and the correlation of the effects of different VRS genes on lateral spikelet fertility. Using chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq), we identified 213 direct downstream genes of VRS1, including those involved in energy metabolism, hormone pathways, and transcription factors. We also discovered that VRS1 directly binds to the D-class gene HvMADS13 to regulate spikelet fertility. Further analysis of the six-rowed barley accessions revealed that a 1 bp deletion in the C-terminus of VRS1 disrupts its ability to repress transcription, leading to fertility in the lateral spikelets.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}