Plant, Cell & Environment最新文献_第2页

Benzoxazinoid Induction and Secretion in Wheat by Intercropped Faba Bean: Cross-Plant Transfer, Environmental Degradation and Limited Negative Impact on Growth. 间作蚕豆诱导和分泌苯并恶嗪类药物：跨株转移、环境退化和对小麦生长的有限负面影响

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-05-27 DOI: 10.1111/pce.15642

Chaosheng Luo, Huiqiong Ding, Siyin Yang, Yan Dong

{"title":"Benzoxazinoid Induction and Secretion in Wheat by Intercropped Faba Bean: Cross-Plant Transfer, Environmental Degradation and Limited Negative Impact on Growth.","authors":"Chaosheng Luo, Huiqiong Ding, Siyin Yang, Yan Dong","doi":"10.1111/pce.15642","DOIUrl":"https://doi.org/10.1111/pce.15642","url":null,"abstract":"Benzoxazinoids (BXs) synthesised by cereal plants are vital for stress resistance. However, information regarding the induction of specific BXs (DIBOA, DIMBOA, and MBOA) in wheat by typical cereal-legume intercropping systems, such as wheat/faba bean, and their effects on neighbouring intercropping crops, remains limited. To address this knowledge gap, pot and field planting experiments were conducted to examine the influence of intercropped faba bean (IF) on the synthesis and secretion of BXs in wheat, their subsequent absorption by faba bean, and their impact on plant growth. Results showed that under both planting conditions, IF could induce an increase in the concentration of BXs in the shoots (leaves and stems) and underground (roots) parts of intercropped wheat (IW), as well as in the rhizosphere soil (p < 0.05), with the highest concentration in the leaves, reaching up to 78.0 μg/g. The concentration of BXs in various organs of IW was higher under field conditions than under potted conditions, and showed a pattern of leaves > roots > stems > rhizosphere. DIMBOA, induced to synthesise and secrete in the wheat rhizosphere, underwent accelerated degradation and reduced half-life due to the soil environment, which is rich in diverse microorganisms and organic residues. In addition, compared to monoculture faba bean (MF) with trace levels of BXs, all three BXs types were detected in the rhizosphere, roots, and shoots of IF, and all significantly increased (p < 0.05). The BXs absorbed by IF exhibited varying degrees of negative correlation with the growth parameters of wheat and faba bean, but the negative impact on growth was limited. In summary, our research findings enhance the understanding of the secretion of BXs induced by legume crops in cereal-legume intercropping systems and their absorption in interspecific interactions among legume crops.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution of Carbon Dioxide Concentration to the Diurnal Variation in Land Surface Carbon Dioxide Uptake From the Atmosphere. 二氧化碳浓度对陆地表面大气吸收二氧化碳日变化的贡献。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-05-27 DOI: 10.1111/pce.15638

Weize Tang, Fangyue Zhang, Paul C Stoy, Russell L Scott, Angela Che Ing Tang, Zheng Fu

{"title":"Contribution of Carbon Dioxide Concentration to the Diurnal Variation in Land Surface Carbon Dioxide Uptake From the Atmosphere.","authors":"Weize Tang, Fangyue Zhang, Paul C Stoy, Russell L Scott, Angela Che Ing Tang, Zheng Fu","doi":"10.1111/pce.15638","DOIUrl":"https://doi.org/10.1111/pce.15638","url":null,"abstract":"Gross primary productivity (GPP) is the key pathway for CO2 uptake by terrestrial ecosystems. Diurnal variation in atmospheric CO2 concentration ([CO2]) can reach 5%-15%, yet the extent to which (CO2) contributes to diurnal GPP variations across biomes and how this contribution varies across climate gradients remain unclear. Here, we used global half-hourly eddy covariance observations to quantify the importance of (CO2) in driving diurnal GPP variations and investigated the environmental factors influencing this response. On average, the relative contribution of (CO2) to diurnal GPP variations, calculated through multivariate regression, was 11% across all sites, comparable to the contributions of vapour pressure deficit (13%) and soil moisture (11%). We also observed systematic differences in the contribution of (CO2) to GPP variability across ecosystems with different plant functional types and climate conditions. Variation in the contribution of (CO2) to GPP is primarily driven by temperature-related variables. At long-term sites, we identified a significant upward trend in (CO2) influence on GPP over time, suggesting an increasing role of (CO2) in explaining GPP variations. This study quantified the importance of (CO2) to the diurnal variations of GPP across globally distributed ecosystems and highlighted that it makes a significant contribution to diurnal GPP patterns.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ERF072 Transcription Factor Directly Regulates MtSOC1-Like Expression and Mediates Drought-Accelerated Flowering in Medicago truncatula. ERF072转录因子直接调控mtsoc1样蛋白表达并介导干旱加速开花

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-05-27 DOI: 10.1111/pce.15648

Zhiwei Wang, Jingwei Li, Yu Min, Ruyue You, Fan Gao, Xiaomin Ren, Yanyun Zhao, Jiabin Fu, Jun Li, Liquan Zhang, Yiding Niu

{"title":"The ERF072 Transcription Factor Directly Regulates MtSOC1-Like Expression and Mediates Drought-Accelerated Flowering in Medicago truncatula.","authors":"Zhiwei Wang, Jingwei Li, Yu Min, Ruyue You, Fan Gao, Xiaomin Ren, Yanyun Zhao, Jiabin Fu, Jun Li, Liquan Zhang, Yiding Niu","doi":"10.1111/pce.15648","DOIUrl":"https://doi.org/10.1111/pce.15648","url":null,"abstract":"Flowering time is a key agricultural trait that indicates the yield of Medicago truncatula. Although drought stress affects flowering time in this species, the molecular mechanism underlying the enhancement of flowering to facilitate drought tolerance remains unclear. Accelerated flowering at the onset of drought enables drought escape in Medicago truncatula, ethylene-responsive factors are an important class of transcription factors whose members are involved in drought stress processes in numerous plants. In this study, MfERF072 overexpression accelerated flowering in Medicago truncatula. In addition, the knockdown of this gene did not affect flowering time, MfERF072 overexpression enhanced drought and decreased the flowering time of Medicago truncatula under drought stress. Moreover, a more pronounced phenotype was observed. In contrast, the knockdown of this transcription factor reduced drought tolerance and delayed flowering time. Furthermore, yeast one-hybrid and two-luciferase assays confirmed that ERF072 binds directly to the promoter of the flowering integration MtSOC1-like gene in Medicago truncatula. This consequently promotes floral transition under drought conditions. Our preliminarily data revealed that ERF072 regulates flowering under drought stress. These results may provide insights into new genetic resources for the molecular breeding of Medicago truncatula, ultimately supporting stress tolerance by balancing drought stress responses and flowering time.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H₂O₂-Dependent Methyl Jasmonate Regulates H₂S-Induced Resistance to Fusarium oxysporum f. sp. niveum Race 2 in Citrullus lanatus. h2o2依赖性茉莉酸甲酯调控瓜对h2s诱导的尖孢镰刀菌抗性。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-05-27 DOI: 10.1111/pce.15654

Shiyu Li, Tongshu Zhao, Ning Chang, Yi Chen, Qi Wang, Zhongyuan Wang, Chunhua Wei, Jianxiang Ma, Yong Zhang, Xian Zhang, Hao Li

{"title":"H2O2-Dependent Methyl Jasmonate Regulates H2S-Induced Resistance to Fusarium oxysporum f. sp. niveum Race 2 in Citrullus lanatus.","authors":"Shiyu Li, Tongshu Zhao, Ning Chang, Yi Chen, Qi Wang, Zhongyuan Wang, Chunhua Wei, Jianxiang Ma, Yong Zhang, Xian Zhang, Hao Li","doi":"10.1111/pce.15654","DOIUrl":"https://doi.org/10.1111/pce.15654","url":null,"abstract":"Fusarium wilt, caused by Fusarium oxysporum (Fo), is a destructive fungal disease that reduces crop yield and quality. Hydrogen sulphide (H2S), a critical signalling molecule, modulates plant defence responses; however, its role and mechanism in combating Fo remain elusive. This study reveals that exogenous NaHS (an H2S donor) enhances watermelon resistance to Fusarium oxysporum f. sp. niveum race 2 (FON2), accompanied by elevated hydrogen peroxide (H2O2) and methyl jasmonate (MeJA) levels. Exogenous H2O2 and MeJA also enhance FON2 resistance. Conversely, silencing respiratory burst oxidase homologue F (ClRBOHF) and jasmonic acid carboxyl methyltransferase (ClJMT), key genes for H2O2 and MeJA biosynthesis, respectively, inhibits NaHS-induced resistance to FON2. Deletion of l-cysteine desulfhydrase (ClLCD), a pivotal gene for H2S generation, reduces FON2 resistance, but this reduction is restored by H2O2 or MeJA supplementation. Upon FON2 infection, exogenous H2O2 elevates MeJA levels; however, silencing ClRBOHF suppresses NaHS-induced MeJA accumulation. Furthermore, silencing ClClJMT inhibits H2O2-induced FON2 resistance, while MeJA supplementation rescues the reduced resistance caused by ClRBOHF silencing. Collectively, these findings demonstrate that H2O2-dependent MeJA plays a crucial role in regulating H2S-induced watermelon resistance to FON2. The growing focus on reducing pesticide use highlights the potential of this mechanism for combating Fo sustainably.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in Soil Microbiome Mediated by Root Volatiles Enhanced Manganese Tolerance of an Invasive Plant Species. 根挥发物介导的土壤微生物组变化增强了入侵植物对锰的耐受性。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-05-27 DOI: 10.1111/pce.15644

Jieren Jin, Tiantian Lin, Danfeng Liu, Yi Wang, Xiao Xu, Yunjian Xu, Evan Siemann, Bo Li

{"title":"Changes in Soil Microbiome Mediated by Root Volatiles Enhanced Manganese Tolerance of an Invasive Plant Species.","authors":"Jieren Jin, Tiantian Lin, Danfeng Liu, Yi Wang, Xiao Xu, Yunjian Xu, Evan Siemann, Bo Li","doi":"10.1111/pce.15644","DOIUrl":"https://doi.org/10.1111/pce.15644","url":null,"abstract":"Many invasive plants exhibit high heavy metal tolerance, but the roles of root-associated soil microbiomes in this process remain poorly understood. Heavy metal stress can alter the release of plant volatile organic compounds (VOCs), potentially influencing plant-soil feedbacks. This study utilised an aggressive invasive plant species Phytolacca americana as a study model, to assess the effects of different levels of soil manganese (Mn) stress on the emissions of root VOCs, and their subsequent influence on soil microbial communities. Results obtained here indicated that elevated Mn stress levels notably increased the quantity and altered the composition of root VOCs, subsequently influencing the diversity and composition of soil microbiomes. Specifically, a decrease in bacterial diversity and an increase in beneficial bacterial genera were observed. Limonene was identified as a key VOC compound influencing bacterial community composition, potentially promoting the accumulation of beneficial bacterial taxa such as Bacillus in soil. Reintroduction of inoculated soil collected from Mn-stressed plants significantly enhanced the tolerance of P. americana to Mn treatment. Elemental analysis suggested that the improved plant tolerance to Mn following soil reintroduction may be attributed to enhanced nutrient uptake that may be facilitated by beneficial microorganisms rather than reduced Mn accumulation in plant tissues.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Key Determinants of Seed Size for Enhancing Genetic Gain in Legumes. 提高豆科植物遗传增益的种子大小的关键决定因素。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-05-23 DOI: 10.1111/pce.15632

Asish Kumar Padhy, Ananya Singh, Shiksha Chaurasia, Swarup Kumar Parida, Kuldeep Tripathi, Sabhyata Bhatia

{"title":"Key Determinants of Seed Size for Enhancing Genetic Gain in Legumes.","authors":"Asish Kumar Padhy, Ananya Singh, Shiksha Chaurasia, Swarup Kumar Parida, Kuldeep Tripathi, Sabhyata Bhatia","doi":"10.1111/pce.15632","DOIUrl":"https://doi.org/10.1111/pce.15632","url":null,"abstract":"Legumes play a pivotal role in human nutrition due to their high nutritional value, especially protein content. Therefore, enhancing the productivity of grain legumes is desirable for ensuring food and nutritional security. Seed size and seed weight are key factors influencing productivity. This article consolidates the substantial amount of research conducted to uncover the molecular signatures associated with seed size into a structured format, providing a one-stop platform of available resources for enhancing genetic gains in legumes. The advent of NGS technologies enabled the decryption of genomes and transcriptomes of important grain legumes. Moreover, molecular signatures such as SSRs, SNPs, transcription factors, methylation patterns and so forth scanned from phenotypically and genotypically well-characterized natural and mapping populations helped identify the QTLs, MTAs and candidate genes associated with seed size. Many of these QTLs and candidate genes have been utilized in marker-assisted breeding for achieving larger seeds and enhanced yield in legumes. Besides, the characterization of legume orthologs of candidate genes from other crops using different omics approaches helped in understanding the regulatory pathways involved in seed size determination in legumes. This review provides a direction for the effective utilization of available resources to enhance legume productivity.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Additive Interactions Between Multiple Mutualists and Host Plant Genotype Simultaneously Promote Increased Plant Growth and Pathogen Defence. 多共生体与寄主基因型之间的非加性相互作用同时促进植物生长和抗病能力的提高。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-05-23 DOI: 10.1111/pce.15631

Amanda H Rawstern, Lucas J Carbajal, Tyler J Slade, Michelle E Afkhami

{"title":"Non-Additive Interactions Between Multiple Mutualists and Host Plant Genotype Simultaneously Promote Increased Plant Growth and Pathogen Defence.","authors":"Amanda H Rawstern, Lucas J Carbajal, Tyler J Slade, Michelle E Afkhami","doi":"10.1111/pce.15631","DOIUrl":"https://doi.org/10.1111/pce.15631","url":null,"abstract":"Understanding the impact of microbial interactions on plants is critical for maintaining healthy native ecosystems and sustainable agricultural practices. Despite the reality that genetically distinct plants host multiple microbes of large effect in the field, it remains unclear the extent to which host genotypes modulate non-additive microbial interactions and how these interactions differ between benign/pathogenic environments. Our study fills this gap by performing a large-scale manipulative microbiome experiment across seven genotypes of the model legume Medicago truncatula. We combine plant performance metrics, survival analyses, predictive modelling, RNA extractions and targeted gene expression to assess how host genotype and microbes non-additively interact to shape plant growth and disease ecology. Our results reveal three important findings: (1) host genotypes with high tolerance to pathogens benefit more from multiple mutualist interactions than susceptible genotypes, (2) only high-tolerance genotypes retain the same beneficial host performance outcomes from the benign environment within the pathogenic environment and (3) the quality of the symbiotic relationship with mutualists is a strong predictor of host survival against pathogenic disease. By applying these findings towards developing crops that promote synergistic microbial interactions, yields and pathogen defence could be simultaneously increased while reducing the need for toxic fertilisers and pesticides.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tolerance to Phosphorus Deficiency Improves Seed Phytic Acid-to-Iron Molar Ratios in Common Bean. 对磷缺乏的耐受性提高了普通豆种子植酸与铁的摩尔比。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-05-23 DOI: 10.1111/pce.15627

Barbara Karpinska, Christine H Foyer

{"title":"Tolerance to Phosphorus Deficiency Improves Seed Phytic Acid-to-Iron Molar Ratios in Common Bean.","authors":"Barbara Karpinska, Christine H Foyer","doi":"10.1111/pce.15627","DOIUrl":"https://doi.org/10.1111/pce.15627","url":null,"abstract":"Although significant advances have been achieved in the biofortification of common beans to overcome deficiencies in Zinc (Zn) and iron (Fe), the mechanisms involved remain poorly understood. We thus explored the relationships between phosphorus nutrition and Zn and Fe accumulation in four bean genotypes (Edar, Nizok, Colorado and Chimbolos) that nominally show differences in seed Fe and Zn accumulation. In contrast to other genotypes, while phosphorus availability had no effect on seed Fe accumulation, phosphorus and phytate levels were decreased in all lines under phosphorus deficiency. Edar plants had a higher seed yield under low phosphorus conditions than the other lines. Analysis of the seed proteome also revealed that Edar is highly resistant to phosphorus deficiency, with similar seed Fe accumulation but lower phytate levels. The phytic acid-to-Fe molar ratios in Edar seeds produced under low phosphorus nutrition were only twice those of the low phytic acid (lpa) beans that have a 90% lower phytic acid content compared to conventional beans. Proteome analysis revealed that sucrose metabolism and phosphate cycling are shifted in the lpa beans. We conclude that a tolerance to phosphate deficiency, particularly in terms of regulation of phosphate cycling and transport pathways, contributes to decreased seed phytic acid-to-Fe molar ratios.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FpCBS Affects a Diverse Range of Functions of Fusarium pseudograminearum Impacting Its Virulence to Wheat. 多氯联苯影响假谷物镰刀菌的多种功能，影响其对小麦的毒力。

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-05-23 DOI: 10.1111/pce.15622

Jie Zhang, Qiuyuan Chai, Paul H Goodwin, Wenqian Zhu, Mingcong Xia, Runhong Sun, Wen Xu, Chao Wu, Yajing Song, Qianqian Dong, Lirong Yang

{"title":"FpCBS Affects a Diverse Range of Functions of Fusarium pseudograminearum Impacting Its Virulence to Wheat.","authors":"Jie Zhang, Qiuyuan Chai, Paul H Goodwin, Wenqian Zhu, Mingcong Xia, Runhong Sun, Wen Xu, Chao Wu, Yajing Song, Qianqian Dong, Lirong Yang","doi":"10.1111/pce.15622","DOIUrl":"https://doi.org/10.1111/pce.15622","url":null,"abstract":"A cystathionine β-synthase (CBS) gene, FpCBS, of the wheat crown rot pathogen, Fusarium pseudograminearum, was identified. FpCBS was highly expressed during conidiation and the early phases of infection. Compared to the wild-type and FpCBS complemented strains, the FpCBS deletion strain had a similar hyphal morphology and growth rate but reduced conidiation. In culture, the FpCBS deletion strain showed broad-scale changes with a decrease in H2S levels, changes in sulphur-containing amino acids and 1207 differentially expressed proteins. The deletion strain also showed reduced deoxynivalenol (DON) production, cellophane penetration ability, antioxidant capacity, as well as increased hyphal iron levels, sensitivity to H2O2 and lipid peroxidation. During infection, the ΔFpcbs strain showed diminished virulence to wheat with fewer penetration structures, and there was a stronger host defence response with wheat epidermal cells having thickened walls and greater reactive oxygen species accumulation. Transcriptomic analysis of infected leaves showed 1954 differentially expressed genes in the FpCBS deletion strain compared to the wild-type strain. This included genes for sulphur metabolism, antioxidative defence, plant cell wall-degrading enzymes and trichothecene production. FpCBS not only plays a pivotal role in sulphur metabolism and H2S production, but it is also important for a broad range of fungal functions, including iron homoeostasis, mycotoxin production and development (conidiation and penetration structures). These factors may all contribute to varying extents to the significant decrease in virulence of the FpCBS deletion strain. The findings have shown that FpCBS is important for much more than sulphur metabolism and H2S synthesis. However, its role in virulence highlights its potential as a novel target for developing strategies to control wheat crown rot.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RsKNAT3 Interacts Antagonistically With RsKNAT1 to Confer Thermotolerance by Regulating RsDREB2A Transcription in Radish. RsKNAT3通过调控RsDREB2A转录与RsKNAT1拮抗作用赋予萝卜耐热性

IF 6 1区生物学

Plant, Cell & Environment Pub Date : 2025-05-21 DOI: 10.1111/pce.15619

Kai Wang, Jingxue Li, Yan Wang, Liang Xu, Feng Cui, Yi Mei, Jiali Ying, Bingshuang Li, Xiaoli Zhang, Shilin Ma, Ruixuan Wang, Nannan Liao, Liwang Liu

{"title":"RsKNAT3 Interacts Antagonistically With RsKNAT1 to Confer Thermotolerance by Regulating RsDREB2A Transcription in Radish.","authors":"Kai Wang, Jingxue Li, Yan Wang, Liang Xu, Feng Cui, Yi Mei, Jiali Ying, Bingshuang Li, Xiaoli Zhang, Shilin Ma, Ruixuan Wang, Nannan Liao, Liwang Liu","doi":"10.1111/pce.15619","DOIUrl":"https://doi.org/10.1111/pce.15619","url":null,"abstract":"Knotted1-like homeobox (KNOX) transcription factors (TFs) are widely involved in plant growth and development processes, including shoot apical meristem division and leaf and root organ development. However, the critical roles of KNOXs in response to abiotic stress, especially heat stress (HS), remain largely unexplored in plants. In this study, both the transcriptome and RT-qPCR analysis revealed that two KNOX TFs, RsKNAT1 and RsKNAT3, were significantly responsive to HS in radish (Raphanus sativus L.). RsKNAT3, highly expressed in heat-tolerant genotypes, enhances thermotolerance and ROS scavenging, acting as a transcriptional activator. Conversely, RsKNAT1, elevated in heat-susceptible genotypes, negatively regulates thermotolerance and increases ROS accumulation, functioning as a repressor. Interestingly, the RsKNAT1 interacts with RsKNAT3 by forming a heterodimer. The identification of regulatory elements showed that RsKNAT3 bound to the Knotted I binding site and activate RsDREB2A expression, while RsKNAT1 acts as the specific repressor of RsKNAT3 and inhibits the regulation of RsKNAT3-targeted RsDREB2A under HS. These findings provided insights into the regulatory mechanism underlying thermotolerance mediated by RsKNAT3 interacting antagonistically with RsKNAT1, facilitating the genetically improvement of the heat tolerance in radish breeding programs.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0