Yanshuo Li, Kai Gao, Bin Liu, Yi Feng, Ziyan Nie, Yunxiao Zhao, Qinghua Liu, Minyan Wang, Wei Li, Yangdong Wang, Hengfu Yin
{"title":"A Molecular Link in the Defence Response of Pinus massoniana to Pine Wilt Disease: Interactions Between TIFY Family Genes and the Nematode Effector.","authors":"Yanshuo Li, Kai Gao, Bin Liu, Yi Feng, Ziyan Nie, Yunxiao Zhao, Qinghua Liu, Minyan Wang, Wei Li, Yangdong Wang, Hengfu Yin","doi":"10.1111/pce.70436","DOIUrl":"10.1111/pce.70436","url":null,"abstract":"<p><p>In response to pine wood nematode (PWN) invasion, pine trees can activate immune responses involving effector-triggered signalling, leading to redox imbalance and programmed cell death. However, such hypersensitive responses are also implicated in disrupting water conduction and accelerating wilting. Despite the identification of several PWN effectors, their roles in modulating host immunity remain unclear. Using integrated transcriptomic and metabolomic analyses across PWN infection timepoints, we revealed that the jasmonate (JA) pathway played a central role in the induction of defence responses; the expression levels of genes involved in JA biosynthesis and signal transduction changed markedly at different stages of PWN invasion. Through yeast two-hybrid screening, we revealed that the PWN effector BxCDP1 interacts with PmTIFY8, a key transcriptional regulator of the Jasmonate ZIM-domain (JAZ) family in the JA signalling pathway. We further demonstrated that this interaction occurs in the nucleus, attenuates reactive oxygen species (ROS)-mediated cell death, and modulates the expression of JA-responsive genes. Our results indicate that the interaction of BxCDP1 with JAZ proteins can impede the JA-mediated immune responses, which is a key link of PWN pathogenicity and provides information for genetic improvement to enhance resistance in pine trees.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"2952-2969"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146140572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chanjuan Ye, Dagang Chen, XinQiao Zhou, Jie Guo, Lu Dai, Xinyi Lin, Hongbo Li, Yuanhao Ma, Rui Cao, Bin Jia, Shu Jiang, Yanduan Hu, Yi Zou, Juan Liu, Qixing Zheng, Hai Zhang, Guo Tao, Ke Chen, Chuanguang Liu
{"title":"GW2 Interacts With OsPIP2;1 to Control Cell Division and Expansion in Rice.","authors":"Chanjuan Ye, Dagang Chen, XinQiao Zhou, Jie Guo, Lu Dai, Xinyi Lin, Hongbo Li, Yuanhao Ma, Rui Cao, Bin Jia, Shu Jiang, Yanduan Hu, Yi Zou, Juan Liu, Qixing Zheng, Hai Zhang, Guo Tao, Ke Chen, Chuanguang Liu","doi":"10.1111/pce.70469","DOIUrl":"10.1111/pce.70469","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3335-3339"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147323995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A BolMYB34-l-BolTRY-l Regulatory Module Negatively Regulates Trichome Initiation in Brassica oleracea.","authors":"Jingru Guan, Qi Li, Wentao Hu, Xiao Ma, Kui Li, Zhimin Wang, Wei Qian, Jiaqin Mei, Qinglin Tang, Dayong Wei","doi":"10.1111/pce.70459","DOIUrl":"10.1111/pce.70459","url":null,"abstract":"<p><p>Brassica oleracea exhibits remarkable morphological diversity and is cultivated worldwide. We previously showed that dense trichomes in a wild accession (B. incana, C01) confer resistance to insect pests, yet the underlying regulatory mechanisms remain unclear. Here, we developed a cleaved amplified polymorphic site (CAPS) marker to distinguish sequence variations in BolTRY-l between trichome-rich C01 and glabrous C41. Functional analyses revealed that overexpression of BolTRY-l in the Arabidopsis try mutant and in C01 markedly suppressed trichome initiation, identifying BolTRY-l as a negative regulator. Promoter sequence comparison and activity assays further indicated that divergence in the BolTRY-l promoter underlies the contrasting trichome phenotypes between C01 and C41. Using the HDOCK server, yeast one-hybrid (Y1H), and dual-luciferase reporter (Dual-LUC) assays, we showed that BolMYB34-l directly binds to MYB-binding site (MBS) elements within the BolTRY-l promoter in C01. In addition, co-immunoprecipitation (Co-IP), bimolecular fluorescence complementation (BiFC), and luciferase complementation (LCA) assays confirmed a physical interaction between BolMYB34-l and BolTRY-l. Overexpression and virus-induced gene silencing (VIGS) analyses further supported that BolMYB34-l functions as an upstream negative regulator of BolTRY-l. Collectively, our findings reveal a novel regulatory module in which the BolMYB34-l-BolTRY-l complex negatively regulates trichome formation in B. oleracea.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3223-3236"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"TCP15 and PIF4 Target the Sulfopeptide-Encoding Gene GOLVEN1/CLE-LIKE6 to Repress Anthocyanin Accumulation.","authors":"Rocío M Jure, Ivana L Viola, Daniel H Gonzalez","doi":"10.1111/pce.70451","DOIUrl":"10.1111/pce.70451","url":null,"abstract":"<p><p>Anthocyanins are protective pigments synthesised by plants to cope with several stressful situations. Anthocyanin synthesis is tightly controlled by multiple transcriptional mechanisms involving the action of activators and repressors. In this work, we report that the class I TCP transcription factor TCP15 and the Phytochrome interacting factor PIF4 negatively affect anthocyanin synthesis by directly inducing the expression of the gene encoding the sulfopeptide GOLVEN1/CLE-LIKE6 (GLV1/CLEL6), a recently identified repressor of anthocyanin synthesis in Arabidopsis thaliana. These transcription factors bind to a region of the GLV1/CLEL6 promoter containing nearby TCP-box and G-box motifs and are able to activate the expression of a reporter gene located under the control of the GLV1/CLEL6 promoter, supporting a direct regulation. Moreover, PIF4 binding to the GLV1/CLEL6 promoter is compromised in a double mutant of TCP15 and the related TCP gene TCP14, whereas TCP15 activation of the GLV1/CLEL6 promoter depends on PIF function, revealing a functional interdependence between these transcription factors. Furthermore, we found that TCP15 and PIF4 also participate in gibberellin-dependent repression of anthocyanin biosynthesis, acting at different levels but independently of GLV1/CLEL6. Altogether, our results add new information on the molecular mechanisms involved in the regulation of anthocyanin accumulation in plants.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3101-3114"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146217934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multimodal Dissection of UV-B-Induced Plant Defense Against Insect in Tea Plants.","authors":"Yali Bi, Liangxin Duan, Yunfei Shi, Jiaming Liu, Haoyi Wu, Tingting Jing, Zhiyuan Liu, Xuefeng Hu, Jingjing Chen, Haojie Xia, Wei Zhang","doi":"10.1111/pce.70441","DOIUrl":"10.1111/pce.70441","url":null,"abstract":"<p><p>Sustainable agriculture urgently requires innovative, pesticide-free strategies to mitigate herbivory and safeguard food security. Ultraviolet-B (UV-B) irradiation, with tunable intensity and cost-effectiveness, has emerged as a promising non-chemical method to enhance plant resistance, yet its underlying mechanisms remain elusive. Here, using tea plant (Camellia sinensis) and its major pest Ectropis obliqua as a model, we developed a multimodal framework that integrates AI-enhanced electronic nose technology for real-time volatile profiling with in situ hyperspectral stimulated Raman scattering (SRS) microscopy to characterize defense responses under precisely controlled UV-B treatments. This approach identified herbivore-induced volatiles-hexanal, (Z)-3-hexenol, octanal, and (Z)-3-hexenyl acetate-optimally induced at 1.2 kJ·m<sup>-2</sup> UV-B and linked to insect deterrence. SRS imaging further revealed elevated jasmonic acid derivatives and L-phenylalanine, coupled with reduced protein levels and altered stomatal dynamics, all correlating with enhanced resistance. Transcriptomic and molecular analyses confirmed transcriptional regulation of these pathways. By bridging volatile detection, metabolic imaging, and molecular validation, this study pioneers a multimodal strategy that provides mechanistic insights into UV-B-mediated plant defense and highlights the potential of multimodal methodologies as powerful tools for developing sustainable, pesticide-free pest management solutions in precision agriculture.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3056-3069"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146197191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Versatile Role of RNA N<sup>6</sup>-Methyladenosine (m<sup>6</sup>A) in Plant Resistance to Biotic Stress.","authors":"Meiqiu Xu, Feifan Zhang, Junjiang Chen, Meiqi Zhang, Olivier Songue Same, Lunji Wang, Guillaume Legrand Ngolong Ngea","doi":"10.1111/pce.70449","DOIUrl":"10.1111/pce.70449","url":null,"abstract":"<p><p>Concerns about biotic stress in agriculture have recently increased with the emergence of persistent pathogens and pests. N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) RNA is a conserved epitranscriptomic modification. Recent advances in plant biotechnology and m<sup>6</sup>A profiling have generated unprecedented knowledge. Our review emphasizes recent state-of-the-art reports regarding m<sup>6</sup>A modulation of plant responses to biotic stress. We found that m<sup>6</sup>A modification plays a \"master rheostat\" role in plant immunity, potentially integrating signaling, transcription, protein turnover, and global metabolic pathways to achieve vigorous, as well as balanced, responses to biotic stress. This review highlights the potential for m<sup>6</sup>A to dynamically modulate interactions between plant defense hormones and defense pathways. m<sup>6</sup>A modulates the stability and activity of transcription factors, regulates defense proteins, antimicrobial metabolite production, antiviral defense, systemic acquired resistance, and the ubiquitin-proteasome pathway. Our review examines contextual factors that coordinate the activity of m<sup>6</sup>A-associated proteins and modulate global m<sup>6</sup>A dynamics. Importantly, we have addressed m<sup>6</sup>A in the context of promising trade-offs between defense and growth, and in the role of m<sup>6</sup>A-associated proteins in liquid-liquid phase separation to control hormonal transcript levels and fine-tune the plant defense response. Overall, this review proposes a new horizon for developing more biotic-stress-resilient plants.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3159-3188"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147269165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xueyuan Sheng, Yanfei Wang, Qi An, Chang Chen, Shuangchao Wang, Endong Wang, Yan Zhao, Lihua Guo
{"title":"Mycovirus-Induced Functional Reprogramming of a Plant Pathogenic Fungus for Biocontrol of Western Flower Thrips.","authors":"Xueyuan Sheng, Yanfei Wang, Qi An, Chang Chen, Shuangchao Wang, Endong Wang, Yan Zhao, Lihua Guo","doi":"10.1111/pce.70485","DOIUrl":"10.1111/pce.70485","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3433-3435"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147375544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shefali Mishra, Diksha Bisht, Anna Amtmann, Ashish K Srivastava, Girdhar K Pandey
{"title":"Potassium Deficiency and Hormone Signalling in Plants.","authors":"Shefali Mishra, Diksha Bisht, Anna Amtmann, Ashish K Srivastava, Girdhar K Pandey","doi":"10.1111/pce.70165","DOIUrl":"10.1111/pce.70165","url":null,"abstract":"<p><p>Potassium (K or K⁺) is a vital macronutrient that influences numerous physiological processes related to plant physiology and development. Recently, there is a growing focus on enhancing K<sup>+</sup>-use efficiency (KUE) to ensure optimal plant growth, especially in K<sup>+</sup> deficient soils. Most approaches are centred on targeting genes associated with K<sup>+</sup> sensing, signalling and other pathways related to plant hormones. However, despite progress, the success stories for generating high KUE crops are still limited. In view of this, the present review highlights the role of hormonal signalling in regulating K<sup>+</sup> deficiency-induced responses in plants. We integrate shreds of evidence of how these K<sup>+</sup>-hormone signalling crosstalk modulate root-system architecture, K⁺ uptake and stress resilience. Furthermore, a meta-analysis-based assessment of different hormones highlighted the central role of jasmonic acid and abscisic acid in mediating K⁺ deficiency-induced changes at the transcriptional level. The present review offers novel insights into K<sup>+</sup>-hormone crosstalk that can be used as a framework for advancing KUE research and addressing challenges towards global food security.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"2935-2951"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The SlHY5-SlbZIP17/SlMYC2-SlβCA3 Module Regulates Tomato Defence Against Pst DC3000 Under Low Light Stress.","authors":"Yue Wang, Chunxin Liu, He Zhang, Dalong Li, Xiangyang Xu, Tingting Zhao","doi":"10.1111/pce.70439","DOIUrl":"10.1111/pce.70439","url":null,"abstract":"<p><p>Low light stress can aggravate the occurrence of plant bacterial diseases. However, how the basic leucine zipper (bZIP) family regulates the synergistic regulatory network induced by low light stress and pathogen infection at the transcriptional level is still unclear. In this study, we identified SlbZIP17, which has a dual response to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and low light conditions. It revealed that SlbZIP17 reduces tomato (Solanum lycopersicum) disease symptoms by activating the positive regulator gene SlβCA3 of Pst DC3000 and increasing the ability of plants to scavenge reactive oxygen species (ROS). Further studies revealed that SlbZIP17 forms a heterodimer with SlMYC2 to synergistically amplify the transcriptional activation effect on SlβCA3. Moreover, under low light stress, SlHY5 was inhibited, which directly reduced SlbZIP17 and SlβCA3 expression. This process led to a decrease in the interaction of SlbZIP17 and SlMYC2, which reduced the ability of the transcription complex to activate SlβCA3 and thereby increasing the severity of disease symptoms induced by Pst DC3000. These findings offer insights into the mechanism underlying the low light stress-pathogen interaction and provide new targets for improving resistance in tomato varieties for facility cultivation agriculture.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"2986-3002"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Epigenetic Rewiring Shapes Divergent Genome Plasticity in Virus Response Circuitry in Soybean.","authors":"Ting Fang, Wenxuan Huang, Yueying Wu, Guozheng Xiao, Yongguo Xue, Min Liu, Jingyu Peng, Xinlei Liu, Jingjing Hou, Zhicheng Dong, Meixia Zhao, Lianjun Sun","doi":"10.1111/pce.70446","DOIUrl":"10.1111/pce.70446","url":null,"abstract":"<p><p>Epigenetic modifications play pivotal roles in regulating plant adaptive responses to viral infection and various other stresses. However, how viral infection rewires and shapes chromatin-based epigenetic regulatory networks in crops with contrasting resistance remains unclear. To this end, we investigated the consequences of epigenetic variations in resistant and susceptible soybean cultivars following soybean mosaic virus (SMV) infection. SMV infection mediates the depletion of 24-nucleotide small interfering RNAs (24-nt siRNAs) in susceptible cultivars and induces the accumulation of 24-nt siRNAs in resistant cultivars. Twenty-four-nucleotide siRNA-dependent DNA methylation variable regions are preferentially enriched in euchromatic CHH contexts, and highly variable DNA methylation regions in heterochromatic long terminal repeat (LTR) retrotransposons are independent of 24-nt siRNAs. Moreover, SMV infection triggers extensive chromatin remodelling in susceptible cultivar, where the depletion of 24-nt siRNAs is related to reduced chromatin accessibility. Conversely, SMV infection mildly remodels chromatin accessibility at heterochromatic LTR retrotransposons in the resistant cultivar. Variations in 24-nt siRNA levels and DNA methylation in upstream regions of autophagy-related genes in susceptible cultivars may influence their expression. Our work provides insights into SMV-triggered divergent epigenetic regulatory networks in soybeans with contrasting resistance and provides a valuable foundation for investigating gene regulatory programmes based on epigenetic variations.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3115-3133"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146217748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}