Plant, Cell & Environment最新文献_第3页

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-10-07 DOI: 10.1111/pce.70236

Chenyang Hao, Lixia Zhu, Xiuxiu Li, Longxin Wang, Jince Song, Xiaoyu Zhao, Xiaochun Qin

引用次数: 0

Arabidopsis Heterotrimeric G Beta Variants Shape Plant Development and Modulate Responses to Endoplasmic Reticulum Stress and Salt Stress. 拟南芥异三聚体G β变异体影响植物发育并调节对内质网胁迫和盐胁迫的响应。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-10-06 DOI: 10.1111/pce.70220

Yueh Cho

{"title":"Arabidopsis Heterotrimeric G Beta Variants Shape Plant Development and Modulate Responses to Endoplasmic Reticulum Stress and Salt Stress.","authors":"Yueh Cho","doi":"10.1111/pce.70220","DOIUrl":"https://doi.org/10.1111/pce.70220","url":null,"abstract":"Heterotrimeric G-protein signaling underpins plant growth and stress adaptation, yet the full functional scope of the sole Arabidopsis Gβ subunit, AGB1, has remained unclear. We show that alternative splicing generates four isoforms with nonredundant roles. Full-length AGB1.1 resides at the plasma membrane and endoplasmic reticulum (ER), forms high-affinity dimers with all three Gγ subunits (AGG1-3) and completely rescues the developmental and abiotic-stress defects of agb1 null plants. AGB1.4, lacking part of the N-terminal coiled-coil, retains strong Gγ binding and affords partial rescue. By contrast, AGB1.2 and AGB1.3 show weak or transient Gγ interactions, reflecting missing coiled-coil/WD40 elements, and do not restore chronic-stress phenotypes. Nevertheless, each truncated variant confers niche advantages: AGB1.2 is rapidly induced by tunicamycin, accumulates in nuclei and mitigates early ER damage, whereas AGB1.3 associates with chloroplast margins and improves survival under moderate or delayed salinity stress. Collectively, the four isoforms expand potential Gβγ combinations from three to twelve, thereby diversifying plant G-protein outputs without gene family expansion. These findings provide a mechanistic framework whereby alternative splicing, rather than gene duplication, endows plants with flexible G-protein signaling modules to balance development and environmental resilience.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seed-Borne Spirosoma pollinicola in Commercial Hazelnuts: A Global Survey of Microbial Presence and Allergen Diversity. 商品榛子种子传粉螺旋体：微生物存在和过敏原多样性的全球调查。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-10-06 DOI: 10.1111/pce.70225

Barbara Karpinska, Alessandro Fiocchi, Marta Biolatti, Ileana Manera, Christine Helen Foyer

引用次数: 0

BolMYB28-BolMAM1 Confers Salt and Drought Tolerance via Regulating Specific Aliphatic Glucosinolate Biosynthesis and Affecting ABA Accumulation in Broccoli. BolMYB28-BolMAM1通过调节特定脂肪族硫代葡萄糖苷生物合成和影响ABA积累，赋予西兰花耐盐和耐旱性。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-10-06 DOI: 10.1111/pce.70228

Lixia He, Mengwei Xi, Jiaying Qu, Yu Li, Chengrong Yun, Xu Zhang, Hui Li, Hanmin Jiang, Chunguo Wang

{"title":"BolMYB28-BolMAM1 Confers Salt and Drought Tolerance via Regulating Specific Aliphatic Glucosinolate Biosynthesis and Affecting ABA Accumulation in Broccoli.","authors":"Lixia He, Mengwei Xi, Jiaying Qu, Yu Li, Chengrong Yun, Xu Zhang, Hui Li, Hanmin Jiang, Chunguo Wang","doi":"10.1111/pce.70228","DOIUrl":"https://doi.org/10.1111/pce.70228","url":null,"abstract":"Glucosinolates (GSLs) are secondary metabolites popularly existing in Brassicaceae. However, the role and regulation of GSLs in environmental stress response remain ambiguous. Here, Methylthioalkylmalate synthase 1 (BolMAM1), a GSL biosynthesis-associated gene, was identified in broccoli. The overexpression of BolMAM1 enhanced both salt and drought tolerance in broccoli, along with an increase in aliphatic GSLs, such as 2(R)-hydroxy-3-butenyl GSL (progoitrin). Progoitrin could induce ABA accumulation in vitro. Consistently, ABA homoeostasis was modulated to accumulate more ABA in OEX-BolMAM1 transgenic broccoli. Moreover, sulforaphane (SFN), one of the hydrolysates of aliphatic GSLs, also exhibited accelerated accumulation in OEX-BolMAM1 transgenic broccoli. External application of SFN could rapidly induce stomatal closure. Furthermore, BolMYB28 was demonstrated to directly bind to the promoter of BolMAM1 and activate its transcription. These results indicated that BolMYB28-BolMAM1 confers tolerance to salt and drought stresses mainly by accelerating the biosynthesis of certain specific aliphatic GSLs. Progoitrin is a newly reported GSL positively regulating ABA accumulation. SFN may act as a signaling molecule to regulate stomatal behavior. These findings reveal the role of BolMYB28-BolMAM1 in abiotic stress response via regulating aliphatic GSL biosynthesis, and suggest the applications of progoitrin and SFN as natural defence factors to resist abiotic stresses in plants.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating Load-Cell Lysimetry and Machine Learning for Prediction of Daily Plant Transpiration. 结合负载细胞溶析法和机器学习预测植物每日蒸腾。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-10-05 DOI: 10.1111/pce.70222

Shani Friedman, Nir Averbuch, Tifferet Nevo, Menachem Moshelion

{"title":"Integrating Load-Cell Lysimetry and Machine Learning for Prediction of Daily Plant Transpiration.","authors":"Shani Friedman, Nir Averbuch, Tifferet Nevo, Menachem Moshelion","doi":"10.1111/pce.70222","DOIUrl":"https://doi.org/10.1111/pce.70222","url":null,"abstract":"We conducted research to predict daily transpiration in crops by utilising a combination of machine learning (ML) models combined with extensive transpiration data from gravimetric load cells and ambient sensors. Our aim was to improve the accuracy of transpiration estimates. Data were collected from hundreds of plant specimens growing in two semi-controlled greenhouses over 7 years, automatically measuring key physiological traits (serving as our ground truth data) and meteorological variables with high temporal resolution and accuracy. We trained Decision Tree, Random Forest, XGBoost and Neural Network models on this data set to predict daily transpiration. The Random Forest and XGBoost models demonstrated high accuracy in predicting the whole plant transpiration, with R2 values of 0.89 on the test set (cross-validation) and R2 = 0.82 on holdout experiments. Ambient temperature was identified as the most influential environmental factor affecting transpiration. Our results emphasise the potential of ML for precise water management in agriculture, and simplify some of the complex and dynamic environmental forces that shape transpiration.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145230967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Temperature Disrupts Maize Silk Function Through Metabolic and Oxidative Dysregulation. 高温通过代谢和氧化失调破坏玉米丝的功能。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-10-02 DOI: 10.1111/pce.70201

Mayang Liu, Yingda Huang, Zheng Li, Xuanlong Lv, Mingqi Gu, Shuhua Liao, Xin Dong, Yingbo Gao, Zhen Gao, Pu Wang, Shoubing Huang

{"title":"High Temperature Disrupts Maize Silk Function Through Metabolic and Oxidative Dysregulation.","authors":"Mayang Liu, Yingda Huang, Zheng Li, Xuanlong Lv, Mingqi Gu, Shuhua Liao, Xin Dong, Yingbo Gao, Zhen Gao, Pu Wang, Shoubing Huang","doi":"10.1111/pce.70201","DOIUrl":"https://doi.org/10.1111/pce.70201","url":null,"abstract":"High temperature (HT, ≥ 38°C) impairs maize (Zea mays L.) yield by disrupting pollination, yet mechanisms in female reproductive organs remain elusive. Maize silks, the essential tissues for pollen capture and pollen tube growth, are particularly sensitive to HT, are highly vulnerable to HT. Here, we combined phenotypic, physiological, metabolic and transcriptomic analyses under controlled HT (40/30°C) and control (32/22°C) conditions to dissect mechanisms underlying HT-induced silk growth inhibition (SGI) and silk pollination dysfunction (SPD). HT reduced silk emergence by ~20% but decreased seed set by ~50%, indicating SPD dominated kernel loss over SGI. HT significantly downregulated key genes of the silks that encode sucrose transporters, sugars will eventually be exported through transporters and glycolytic enzymes (hexokinase; 6-phosphofructokinase; pyruvate kinase), restricting energy metabolism required for silk elongation and pollen tube growth. Concurrently, HT elevated abscisic acid and indole-3-acetic acid while suppressing zeatin riboside, brassinolide and jasmonic acid levels, collectively driving SGI. SPD was primarily linked to oxidative damage via suppressed flavonoid biosynthesis (chalcone synthase, flavonol synthase and peroxidase) and impaired reactive oxygen species (ROS) scavenging. Specifically, HT induced a negative correlation between ZmARF1 and ZmSOD3 expression, suggesting compromised ROS clearance that exacerbated silk structural damage. These findings provide new insights into the metabolic, hormonal and transcriptional regulatory networks that govern silk thermotolerance, providing potential molecular targets for breeding heat-resilient maize varieties.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PlOBP1/PlDAM-PlSOC1 Module Regulates Bud Dormancy Transition in Response to Low Temperature. PlOBP1/PlDAM-PlSOC1模块调控低温下芽休眠转变

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-10-01 DOI: 10.1111/pce.70218

Xiaobin Wang, Xiaoxuan Chen, Kaijing Zhang, Danqing Li, Lingmei Shao, Tong Xu, Ziming Ren, Qiyao Wang, Junhong Guo, Runlong Zhang, Cong Gao, David P Horvath, Yiping Xia, Jiaping Zhang

{"title":"PlOBP1/PlDAM-PlSOC1 Module Regulates Bud Dormancy Transition in Response to Low Temperature.","authors":"Xiaobin Wang, Xiaoxuan Chen, Kaijing Zhang, Danqing Li, Lingmei Shao, Tong Xu, Ziming Ren, Qiyao Wang, Junhong Guo, Runlong Zhang, Cong Gao, David P Horvath, Yiping Xia, Jiaping Zhang","doi":"10.1111/pce.70218","DOIUrl":"https://doi.org/10.1111/pce.70218","url":null,"abstract":"The transition from bud endodormancy to ecodormancy is triggered by environmental cues, particularly low temperatures. However, the mechanism underlying bud dormancy transition (BDT) is largely unknown. Here, we identified a low-temperature-responsive, MADS-box family SUPPRESSOR OF OVEREXPRESSION OF CO1 (PlSOC1) under both natural and controlled low temperatures, which promotes BDT and confers low chilling requirement trait of herbaceous peony (Paeonia lactiflora). A novel transcription factor, DNA-binding One Zinc Finger (DOF) family OBF BINDING PROTEIN 1 (PlOBP1), was found to bind the AAAAG motif in the PlSOC1 promoter, acting as a negative regulator of BDT. PlOBP1 acts together with Dormancy Associated MADS-box (PlDAM) protein to enhance the transinhibitory effect of PlSOC1. Further gibberellic acid (GA) treatment showed that exogenous GA can replace long-term chilling to promote BDT, likely by inhibiting the expression of PlOBP1 while inducing the expression of PlSOC1. The elevated PlSOC1 forms a complex with PlDAM and inhibits PlDAM activity, further releasing the inhibition on PlSOC1, thereby amplifying PlSOC1 and triggering BDT. Our findings provide mechanistic insights into low-temperature-mediated GA regulation of BDT and reveal a novel role of DOF protein PlOBP1 and its interactions with MADS-box family members in bud dormancy regulation.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-Mediated RNAi of Non-ATPase Regulatory Subunit Confers Soybean Resistance Against Bean Bug, Riptortus pedestris. 植物介导的非三磷酸腺苷酶调控亚基的RNAi赋予大豆对豆虫的抗性。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-10-01 DOI: 10.1111/pce.70227

Manru Xu, Yuanyu Zhang, Jin Zhao, Xiangdong Yang, Huajuan Li, Biao Hu, Jianping Chen, Zongtao Sun, Zhongyan Wei

{"title":"Plant-Mediated RNAi of Non-ATPase Regulatory Subunit Confers Soybean Resistance Against Bean Bug, Riptortus pedestris.","authors":"Manru Xu, Yuanyu Zhang, Jin Zhao, Xiangdong Yang, Huajuan Li, Biao Hu, Jianping Chen, Zongtao Sun, Zhongyan Wei","doi":"10.1111/pce.70227","DOIUrl":"https://doi.org/10.1111/pce.70227","url":null,"abstract":"Riptortus pedestris, is a major pest threatening soybeans and cause the soybean staygreen syndrome, which has recently become a significant and widespread issue in soybean production. Currently, chemical control remains the primary method for managing R. pedestris in the field, but it contributes to environmental pollution and increases the resistance risk. Plant-mediated RNA interference (RNAi) offers a target-specific and eco-friendly alternative for pest control. While RNAi has been shown to effectively control certain Coleoptera, its effects on piercing-sucking insects, such as R. pedestris, remain poorly understood. In this study, we characterized a non-ATPase regulatory subunit 6 (RPN6) from R. pedestris and demonstrated that downregulation of RPN6 expression significantly increased mortality and reduced oviposition. Phylogenetic analysis revealed that while RPN6 is highly conserved across Hemiptera, the double-stranded RNA (dsRNA) targeting RPN6 is species-specific. We subsequently developed dsRPN6-transgenic soybean lines, and feeding bioassays revealed these transgenic soybean lines exhibited high resistance to R. pedestris, with significantly reduced survival and oviposition rates in the pest. Importantly, the transgenic soybeans displayed moderate staygreen symptoms following infestation by R. pedestris, demonstrating that plant-mediated RNAi targeting of RPN6 confers effective resistance against R. pedestris, providing a promising strategy for bean bug management in agricultural practices.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One Primer Pair for All: A Standardized Vector Toolbox for Protein-Protein Interactions and Protein Localization in Plants. 一个引物对所有：一个标准化的载体工具箱，用于蛋白质相互作用和植物蛋白质定位。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-10-01 DOI: 10.1111/pce.70219

Cailin Luo, Lihaitian Wang, Yuqin Wu, Jun Cai, Bin Peng, Jia Chen, Feng Yu, Dousheng Wu

{"title":"One Primer Pair for All: A Standardized Vector Toolbox for Protein-Protein Interactions and Protein Localization in Plants.","authors":"Cailin Luo, Lihaitian Wang, Yuqin Wu, Jun Cai, Bin Peng, Jia Chen, Feng Yu, Dousheng Wu","doi":"10.1111/pce.70219","DOIUrl":"https://doi.org/10.1111/pce.70219","url":null,"abstract":"Understanding protein-protein interactions (PPIs) is crucial for elucidating the molecular mechanisms underlying plant growth, development, and stress responses. While techniques for analyzing PPIs have been well developed, validating a PPI typically requires a combination of four to five techniques. For each technique, specific primers are designed to clone the gene of interest (GOI) into a given vector. As a result, validating a PPI requires the synthesis of four to five primer pairs, making the process both complex and costly. To overcome these limitations, we have optimized and functionally validated an In-Fusion-based vector toolbox for the flexible construction of PPI vectors in plants. The standardized toolbox facilitates the cloning of a GOI into different PPI vectors using only one primer pair. Furthermore, we extended one of the standardized vectors for protein expression and localization studies. With the same primer pair, a GOI can be easily cloned into vectors with different tags or fluorophores. Our optimized vector toolbox will significantly simplify the assembly of PPI vectors as well as other plant expression vectors for subcellular localization and protein expression.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nighttime Warming Enhances Tree Growth in Temperate Tree Species. 夜间变暖促进温带树种的生长。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-30 DOI: 10.1111/pce.70221

Bingxin Han, Zhaoguo Wang, Di Liu, J Julio Camarero, Maurizio Mencuccini, Binqing Zhao, Yuan Liu, Yushuang Xie, Xiaochun Wang

{"title":"Nighttime Warming Enhances Tree Growth in Temperate Tree Species.","authors":"Bingxin Han, Zhaoguo Wang, Di Liu, J Julio Camarero, Maurizio Mencuccini, Binqing Zhao, Yuan Liu, Yushuang Xie, Xiaochun Wang","doi":"10.1111/pce.70221","DOIUrl":"https://doi.org/10.1111/pce.70221","url":null,"abstract":"Asymmetric warming in temperate regions differentially impacts tree growth depending on whether daytime or nighttime temperatures increase. To elucidate the underlying mechanisms and species-specific responses, we investigated five temperate broadleaf tree species (Juglans mandshurica, Phellodendron amurense, Fraxinus mandshurica, Betula platyphylla and Tilia amurensis) exhibiting contrasting water status regulation strategies and differing in mycorrhizal types. Seedlings of the five species were subjected to five temperature treatments: ambient control, daytime warming (+2°C, DT+2 and +4°C, DT+4) and nighttime warming (+2°C, NT+2 and +4°C, NT+4). Radial growth and xylogenesis were monitored throughout the growing season. Notably, only NT+2 significantly increased ring width (+99.42%) and theoretical hydraulic conductivity (+260.58%) across all species. Nighttime warming enhanced radial growth by increasing the number of cambium and radially enlarging cells, while daytime warming extended the xylem formation period. Furthermore, mean ring width (MRW) increased significantly with radially enlarging cells (maxEC) under nighttime warming. This response pattern was particularly pronounced in arbuscular mycorrhizal and isohydric species, contrasting with ectomycorrhizal and anisohydric species. Collectively, our results demonstrate that the effects of asymmetric warming on tree growth are contingent upon both the amplitude and timing of temperature increases. These findings provide critical mechanistic insights into how asymmetric warming influences tree growth and forest productivity, aiding predictions of carbon sequestration potential under climate change.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145190494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0