Plant, Cell & Environment最新文献

{"title":"Mild Photoperiod and Temperature Fluctuations Elicit Tissue-Specific DNA Methylation Changes in Arabidopsis.","authors":"Ji Hoon Rhee, Hyogi Kim, Hyodong Lee, Yunji Jeon, Yeonhee Choi","doi":"10.1111/pce.70453","DOIUrl":"10.1111/pce.70453","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3151-3154"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146217708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Choose Wisely: Parameter Choice is Key for Ensuring Consistent Estimates of Photosynthetic Capacity From A-C_i Response Curves.","authors":"Josef C Garen, Kristine Y Crous","doi":"10.1111/pce.70462","DOIUrl":"10.1111/pce.70462","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3251-3253"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147281276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-Heading High Nighttime Temperature Impairs Grain Protein-Starch Balance and Rice Quality Through Altering Nitrogen Metabolism.","authors":"Zhechuan Liu, Shengming Xia, Yixiao Li, Hubo Li, Menghao Zhu, Huanran Yin, Zhenmei Wang, Jianguo Man, Dongliang Xiong, Kehui Cui, Jianliang Huang, Shaobing Peng, Shen Yuan, Fei Wang","doi":"10.1111/pce.70437","DOIUrl":"10.1111/pce.70437","url":null,"abstract":"<p><p>High nighttime temperatures (HNT) tend to diminish rice quality by disrupting assimilate translocation and grain filling process in rice (Oryza sativa L.). However, there is controversy remains regarding whether source or sink limitation are the primary driver under HNT during grain filling period. Additionally, the physiological mechanisms underlying the genotypic variation in the response of grain protein content to HNT and its effect on rice quality have been less explored. To address whether nitrogen remobilization from leaves to grains during grain filling determines genotypic differences in grain quality under high night temperature, two cultivars - HHZ (Huanghuazhan, an indica inbred line) and YY4949 (Yongyou4949, an indica-japonica hybrid) - were treated with 30/22°C (day/night, CK) and 30/27°C (HNT) over two consecutive years. Significant genotypic variation in the response of grain storage substances to HNT was observed between the two cultivars. Under HNT, YY4949 exhibited a significant increase in grain protein content and glutelin/prolamin ratio, and this shift negatively impacted rice eating and cooking quality. Notably, the protein/amylose ratio exhibited a stronger correlation with chalkiness degree and pasting characteristics of rice flour. Under HNT, accelerated nitrogen remobilization from leaves to grains in YY4949 - driven by enhanced chloroplast degradation and upregulated expression of nitrogen metabolism-related enzymes and transporters exacerbated source limitation to rice quality and disrupted the balance between starch and protein in grains. Collectively, these findings suggest that genetic modulation of nitrogen remobilization could facilitate the breeding of climate-resilient rice cultivars with superior grain quality.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3039-3055"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146197230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PpMYB14-SAHH1 Module Regulates Peach Fruit Softening Through Promoting Lignin Accumulation.","authors":"Lili Xu, Zhifeng Yang, Hongyang Guo, Aoxing Chen, Xianpu Wang","doi":"10.1111/pce.70448","DOIUrl":"10.1111/pce.70448","url":null,"abstract":"<p><p>Fruit softening critically impacts postharvest quality and storage of peach (Prunus persica L.). To explore the molecular mechanism regulating postharvest peach softening and identify key genes for maintaining fruit quality, this study focused on investigating the function and regulatory network of S-adenosine-L-homocysteine hydrolase 1 (SAHH1). Postharvest peach fruits showed continuous firmness decline and significant anthocyanin accumulation after day 10, indicating a rapid softening initiation. qRT-PCR confirmed PpSAHH1 was strongly positively correlated with firmness (r = 0.648). PpSAHH1 overexpression in peach delayed senescence by maintaining firmness, retarding color change, and preserving cell membrane integrity, while heterologous expression in tomato regulated ripening. Bioinformatics revealed PpSAHH1 is a cytoplasmic acidic soluble protein with conserved structures, evolutionarily close to Prunus dulcis SAHH, and its promoter contains abscisic acid (ABA)-responsive elements. Stable overexpression in apple callus altered cell wall metabolism: reduced pectin and increased cellulose, hemicellulose, and lignin. Transcriptome analysis showed differentially expressed genes enriched in phenylpropanoid biosynthesis, calcium/auxin/ABA signaling, and plant-pathogen interaction. Yeast one-hybrid, dual-luciferase, and GUS assays verified myeloblastosis transcription factor 14 (PpMYB14) directly binds the PpSAHH1 promoter and activates its transcription. Silencing of MdMYB14 in apple significantly reduced the accumulation of total phenols and cell wall metabolic components, particularly lignin content. Collectively, the PpMYB14-SAHH1 module inhibits peach softening by coordinately regulating multiple signaling pathways and phenylpropanoid biosynthesis to promote lignin accumulation, offering new insights and potential targets for postharvest quality improvement.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3137-3150"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146217739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cysteine-Mediated Root Sequestration and Metabolic Reprogramming Alleviate Cadmium Toxicity in Chinese Cabbage.","authors":"Longcheng Li, Xuexian Li, Qiuguo Zhang, Muhammad Ishfaq, Jingyuan Zheng, Wenliang Wu, Qing Chen","doi":"10.1111/pce.70443","DOIUrl":"10.1111/pce.70443","url":null,"abstract":"<p><p>Cadmium (Cd) contamination poses a serious threat to crop productivity and food safety. Although cysteine (Cys) has been implicated in Cd detoxification, its regulatory role in Chinese cabbage remains poorly understood. Here, we demonstrate that exogenous Cys alleviates Cd toxicity by enhancing root Cd retention and coordinating multiple detoxification pathways. Cys restored biomass accumulation, root elongation, and photosynthetic performance while mitigating Cd-induced oxidative damage. Root vigour increased by 44.4%, accompanied by enhanced Cd sequestration in roots and restricted translocation to shoots. Cd concentrations in xylem sap decreased by 42.0%, and shoot Cd accumulation declined by 23.1%-44.6%. Although Cys increased Cd influx and root Cd accumulation, it inhibited long-distance Cd transport by repressing BraIRT1 and BraHMA2 expression. Cys further strengthened vacuolar immobilization by up-regulating BraABCC1/2 and BraPCS1, increasing GSH and PC2-PC4 contents, and improving the GSH/GSSG ratio, thereby supporting Cd chelation and redox homoeostasis. Time-resolved transcriptomics revealed attenuation of Cd-induced sulphate assimilation hyperactivation, enhanced GSH regeneration, and a metabolic shift from lignin biosynthesis toward glycosylated phenylpropanoid detoxification products, as validated by qRT-PCR. Together, these results identify Cys as a practical strategy to reduce shoot Cd accumulation in leafy vegetables by limiting Cd mobility at both cellular and whole-plant levels.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"2970-2985"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146155331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VOC-Mediated Priming Under Copper Stress Alters Metabolite Composition in Scutellaria baicalensis and Neighbouring Seedlings.","authors":"Jun Rao, Simeng Wan, Weiwei Wu, Tao Zi, Jie Chen, Sijia Tang, Feiyan Zhang, Ying Cao, Xinyun Guo, Yating Zhu, Lihua Yao, Hu Su","doi":"10.1111/pce.70467","DOIUrl":"10.1111/pce.70467","url":null,"abstract":"<p><p>Plants under stress emit volatile organic compounds (VOCs) that can influence neighbouring plants. While most studies have focused on the role of VOCs priming the fitness of neighbouring plants, their involvement in priming bioactive compound accumulation in herbal plants remains less clear. In this study, we treated Scutellaria baicalensis Georgi using copper solution, identified the VOCs emitted from S. baicalensis and VOCs accumulated internally in tissues, and quantified total flavonoid and major bioactive compounds. We found that copper treatment inhibited the emissions of acetic acid butyl ester, cyclopentanone and ethyl 2-methylpropanoate. Concurrently, it significantly increased internal levels of 2-propanone and 3-methyl-3-buten-1-ol in aerial parts of both copper-treated (COT) and cocultured untreated (COU) seedlings, alongside a marked decrease in ethanol in COT. In underground parts, the 2-ethyl-1-hexanol and methoxybenzene only presented in COT, the 1-hexanol, 2-acetylfuran and n-pentanal exhibited higher accumulation in both COT and COU. Moreover, copper also enhanced total flavonoid content in COT and, notably, in COU, indicating a priming effect. Individual flavonoids showed a nuanced fluctuation in the aerial parts, but a general decrease in the underground parts. Together, our findings indicate that copper-induced airborne cues, dominated by VOCs, prime neighbouring plants to reconfigure phytochemical profiles, with consequences for herbal quality via shifts in bioactive compound composition.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3324-3334"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147324065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss-of-Function Mutation in TaZIP4-B2 and TaMSH7-3D Fuels Karyotypic Variation, Phenotypic Diversity, and Enables Rapid Evolution of Tolerance to Salinity Stress.","authors":"Tingting Yu, Pengli Gao, Ke Wang, Boshi Chen, Zitong Chu, Shuhan Liu, Jianhui Hong, Xue Zhang, Zhibin Zhang, Ruili Lv, Bao Liu, Hongwei Xun","doi":"10.1111/pce.70487","DOIUrl":"10.1111/pce.70487","url":null,"abstract":"<p><p>Allopolyploid species often contain specific genes dedicated to suppressing meiotic homoeologous pairing. In common wheat, TaZIP4-B2 and TaMSH7-3D fulfil this role. Nevertheless, to what extent the loss-of-function of these genes may lead to meiotic breakdown in wheat itself and hence generate karyotypic heterogeneity remains incompletely understood. Here, we show that CRISPR/Cas9-generated loss-of-function mutation of either or both TaZIP4-B2 and TaMSH7-3D leads to disrupted meiosis, triggering widespread karyotypic instability including both numerical and structural chromosomal variations (NCVs and SCVs). NCVs predominantly occurred in the D subgenome, involving preferential gains of 2A/4B/5D and losses of 6A/5B/2D, while frequencies of SCVs among subgenomes followed the order of subgenomes D > A > B, with 6A/5B/2D showing the most rearrangements. Notably, karyotypic variation in Tazip4-B2/Tamsh7-3D double mutants showed initial rapid accumulation followed by gradual stabilization across generations. Karyotypic heterogeneity caused extensive phenotypic diversity, including several key agronomic traits. Notably, Tazip4-B2/Tamsh7-3D double mutant showed more intercalary insertional translocations than the classical ph1b deletion mutant, suggesting its advantage in alien genetic introgression. Moreover, tolerance to strong salinity emerged in progenies of the mutants due to karyotypic variation. Our findings demonstrate that the loss-of-function mutation of TaZIP4-B2/TaMSH7-3D promotes rapid karyotype variability, phenotypic diversity, and environmental adaptability in wheat itself, suggesting a novel possibility for wheat improvement by karyotypic renovation.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3455-3469"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147429774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Gene Expression Analysis Across Contrasting Linseed Genotypes Identifies New Candidates for Aluminum Toxicity and Low-Phosphorus Tolerance.","authors":"Malemnganbi Keisham, Mazahar Moin, Ng Tombisana Meetei, Mayank Rai, Wricha Tyagi","doi":"10.1111/pce.70447","DOIUrl":"10.1111/pce.70447","url":null,"abstract":"<p><p>In the North East Hill Region (NEHR) of India, acidic soils cause aluminum (Al³⁺) toxicity and phosphorus (Pi) deficiency, severely limiting crop productivity. Linseed offers potential for enhancing cropping intensity and diversification in this region. Contrasting oilseed-type linseed genotypes identified for yield traits under acidic soils were further validated for root and shoot traits through hydroponic screening. Using an orthology-based approach, eight gene families-MATE, ALMT1, MGT1, PT6, PAP15, WD40-38, SPL4, and ACA3-known to confer Al³⁺ toxicity and P deficiency tolerance in other plant species were targeted. Thirty-one genic regions were identified. In roots of four genotypes (Lus 37, Lus 237, Lus 4, and Lus 49) exposed to Al³⁺ toxicity, UGT74, MATE1365, MGT1272, and MGT1577 were upregulated in tolerant genotypes, indicating their probable roles in detoxification, transport, and ion homeostasis. Under low Pi conditions, eleven genes exhibited differential expression in shoots and roots of Lus 37, Lus 29, and Lus 4, with PT6826 and PT6635 upregulated in roots of tolerant Lus 37. Overall, LusMATE1365, LusUGT74, and LusPT6635 were identified as key candidate genes that could potentially confer tolerance to Al³⁺ toxicity and P deficiency. These insights will aid molecular breeding for linseed adaptation to acidic soils of NEHR.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3070-3081"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146197176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen-Mediated Remodelling of Lycium Barbarum Leaf Traits Drives Multimodal Host Selection by Bactericera Gobica.","authors":"Zhao Shiwen, Wei Hongshuang, Qiao Haili, Li Huanle, Liu Sai, Xu Changqing","doi":"10.1111/pce.70428","DOIUrl":"10.1111/pce.70428","url":null,"abstract":"<p><p>In commercial Lycium barbarum production, excessive nitrogen (N) input is associated with outbreaks of the wolfberry psyllid (Bactericera gobica), yet the mechanisms underlying nitrogen-driven population increases remain unclear. Integrating field surveys, pot experiments, behavioural assays, and multi-omics analyses, we show that N supply links shifts in visual/olfactory cues and phloem nutrition to B. gobica population increase. Across nine plantations in three major production regions, infestation severity increased significantly with nitrogen input. High N (HN) reprogrammed chloroplast and carotenoid pathways, producing deeper leaf greenness and elevated apocarotenoids (α-ionone, (E)-β-ionone, and benzeneacetaldehyde) that increased pre-landing encounters and attraction. Concurrently, HN elevated leaf soluble sugars and improved phloem nutritional quality, enhancing post-landing acceptance and feeding. Demographic assays revealed higher oviposition, nymphal abundance, and adult densities on HN plants. Thus, an HN-induced trait suite-enhanced visual/olfactory cues coupled with superior phloem nutrition-promotes host encounter and fecundity, delineating a multimodal bottom-up pathway from fertilisation to outbreak risk. These results provide a mechanistic rationale for optimising N inputs in L. barbarum production and motivate integrating nutrient management with semiochemical- and vision-based monitoring and trapping to reduce pest pressure.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3394-3404"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147375498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome-Level Genome and Organ-Specific Transcriptome of Alnus glutinosa Uncover Lineage-Specific Innovations in Root Nodule Symbiosis.","authors":"Zijian Liu, Xiaoxiao Zhao, Xiuli Li, Yong Feng, Linnan Wu, Zhen Wu, Yao Zhong, Qingcheng Qiu, Bo Song, Hang Zhao, Hongbing Liu, Shifeng Cheng","doi":"10.1111/pce.70440","DOIUrl":"10.1111/pce.70440","url":null,"abstract":"<p><p>Alnus glutinosa is one of only three lineages within the order Fagales capable of establishing root nodule symbiosis (RNS). Although a fragmented genome assembly of A. glutinosa was previously available, its limited quality, combined with the lack of comprehensive transcriptomic resources, has constrained in-depth comparative and functional genomic analyses. In this study, we present a 505 Mb chromosome-level genome assembly of A. glutinosa, anchored to 14 pseudochromosomes, representing the most complete and high-quality genomic resource for this species to date. Whole-genome alignment and synonymous substitution rate (Ks) analysis confirm Alnus and Betula as sister genera with shared genomic architectures and evolutionary histories. Functional enrichment analyses of nodule-enhanced genes reveal significant associations with photosynthesis and sugar metabolism, while expanded gene families are enriched in terpenoid biosynthesis and malate transport pathways, likely critical to RNS in A. glutinosa. Phylogenetic analysis indicated that Alnus has retained non-symbiotic class 1 haemoglobin (nsHB1), but lost nsHB2 haemoglobin, suggesting a lineage-specific adaptation in symbiotic oxygen regulation. Further comparative analysis of nsHB1 protein sequences across nodulating taxa highlights evolutionary patterns within the Alnus lineage. Through a targeted phylogenetic survey of known RNS-related genes, we identified PAV in RPG and copy number variation in AGO5, both of which may underlie Alnus-specific RNS adaptations. Weighted gene co-expression network analysis identified a nodule-specific module comprising 231 genes significantly enriched in sugar-related metabolic pathways. Notably, the bZIP ortholog shows conserved nodule-specific expression across species from Cucurbitales, Rosales and Fabales, suggesting deep evolutionary conservation within the nitrogen-fixing clade. Together, these findings provide a high-resolution view of Alnus-specific RNS adaptations and uncover conserved regulatory modules potentially critical for RNS. These works establish a foundational genomic framework for future efforts aimed at engineering RNS capacity into non-nodulating crops.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"3003-3021"},"PeriodicalIF":6.3,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146176849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}