Plant, Cell & Environment最新文献_第3页

The Arabidopsis deNADding Enzyme DXO1 Modulates the Plant Immunity Response. 拟南芥denadd酶DXO1调控植物免疫应答

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-23 DOI: 10.1111/pce.70199

Anna Golisz-Mocydlarz, Monika Zakrzewska-Placzek, Michal Krzyszton, Nataliia Diachenko, Justyna Piotrowska, Wiktoria Kalbarczyk, Agnieszka Marasek-Ciolakowska, Joanna Kufel

{"title":"The Arabidopsis deNADding Enzyme DXO1 Modulates the Plant Immunity Response.","authors":"Anna Golisz-Mocydlarz, Monika Zakrzewska-Placzek, Michal Krzyszton, Nataliia Diachenko, Justyna Piotrowska, Wiktoria Kalbarczyk, Agnieszka Marasek-Ciolakowska, Joanna Kufel","doi":"10.1111/pce.70199","DOIUrl":"https://doi.org/10.1111/pce.70199","url":null,"abstract":"DXO1, the only DXO homolog in Arabidopsis, due to its plant-specific features, exhibits strong deNADding enzymatic activity but has no apparent role in 5' QC. Despite its important contribution to plant RNA metabolism, the direct impact of DXO1 enzymatic activity on cellular processes appears surprisingly limited. Notably, most molecular and morphological changes observed so far in dxo1 mutant plants depended on the plant-specific N-terminal domain of the protein. Our investigation into the role of DXO1 in response to biotic stress, specifically its susceptibility to Pseudomonas syringae pv. tomato DC3000 infection, unexpectedly revealed the importance of DXO1 enzymatic activity in the plant immune response. We observed that dxo1-2 knockout mutant and transgenic dxo1-2 lines expressing a DXO1 variant either catalytically inactive or lacking the N-terminal domain exhibited enhanced resistance to Pst, accompanied by marked changes in the expression of key pathogenesis markers. Also, other markers of plant immunity, such as callose deposition and production of reactive oxygen species, were strongly induced by PAMPs elf18 and flg22. These results strongly suggest that both DXO1 features, the N-terminal domain and its catalytic site, contribute to the regulation of plant immunity. This is the first observation revealing the involvement of DXO1 enzymatic activity in plant physiology. Moreover, our analyses showed that dxo1-2 mutation altered the expression of a large group of defense-related genes, affected the stability of mRNAs, and delayed the activation of MAP kinases. Therefore, we postulate that DXO1 protein deregulates defense against Pst infection at both the transcriptional and posttranscriptional levels.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root-Derived Trans-Zeatin-Type Cytokinins Increase Cold Tolerance in Rice Seedlings by Regulating Energy Metabolism. 根源反式玉米蛋白型细胞分裂素通过调节能量代谢提高水稻幼苗的抗寒性。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-23 DOI: 10.1111/pce.70190

Yi Yang, Jian Luo, Jingyi Feng, Huihui Hu, Jianke Xiao, Chengqiang Ding, Vinay Nangia, Yang Liu

{"title":"Root-Derived Trans-Zeatin-Type Cytokinins Increase Cold Tolerance in Rice Seedlings by Regulating Energy Metabolism.","authors":"Yi Yang, Jian Luo, Jingyi Feng, Huihui Hu, Jianke Xiao, Chengqiang Ding, Vinay Nangia, Yang Liu","doi":"10.1111/pce.70190","DOIUrl":"https://doi.org/10.1111/pce.70190","url":null,"abstract":"Rice seedlings are highly sensitive to low-temperature stress. Cytokinins are important endogenous signalling molecules in plants and play a critical role in regulating stress responses. However, the mechanism by which cytokinins mediate cold stress responses in rice seedlings remains unclear. In this study, we employed cold-tolerant and cold-sensitive rice cultivars, cytokinin-defective mutants and exogenous cytokinin supplementation to elucidate the mechanisms underlying cytokinin-mediated chilling adaptation. First, we compared the transcriptomic and metabolomic profiles of a cold-tolerant cultivar (HY73) and a cold-sensitive cultivar (WFY286) under low-temperature treatment (11°C for 8 h). The results revealed that cytokinins, along with energy metabolic pathways such as glycolysis and the tricarboxylic acid cycle, are closely associated with cold tolerance in rice seedlings. Compared with WFY286, HY73 presented higher levels of root-derived trans-zeatin (tZ)-type cytokinins in leaves, increased energy metabolism, elevated ATP content and increased energy charge. Furthermore, the tZ-type cytokinins transport-deficient mutant abcg18 presented reduced cold tolerance, lower energy metabolic activity and decreased ATP and energy charge levels, indicating that the transport of tZ-type cytokinins is crucial for cold stress responses. Leaf spraying with tZ significantly improved the energy metabolism and cold tolerance of WFY286 and abcg18. Taken together, our findings suggest that root-derived tZ-type cytokinins enhance low-temperature adaptation in rice seedlings by promoting energy metabolism and maintaining cellular energy homoeostasis. This study provides a theoretical basis for improving cold resistance in rice through manipulation of cytokinin signalling pathways.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Memory of Drought Reshapes Root-Associated Communities to Enhance Plant Resilience. 干旱的微生物记忆重塑根相关群落，增强植物的抗灾能力。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-23 DOI: 10.1111/pce.70200

Hongyin Qi, Xin Wen, Ziyue Wang, Shuxia Yin

{"title":"Microbial Memory of Drought Reshapes Root-Associated Communities to Enhance Plant Resilience.","authors":"Hongyin Qi, Xin Wen, Ziyue Wang, Shuxia Yin","doi":"10.1111/pce.70200","DOIUrl":"https://doi.org/10.1111/pce.70200","url":null,"abstract":"Global climate change has increased the frequency and severity of droughts, posing significant threats to grassland ecosystems. As a dominant species in meadow steppe in northern China's grasslands, Leymus chinensis exhibits excellent drought resistance, yet the interaction mechanisms between its drought resistance and rhizosphere microbial communities remain unclear. This study simulated short-term drought cycles (0-3) and combined high-throughput sequencing with microbial transplantation experiments to investigate rhizosphere and bulk soil microbial responses to drought and their regulatory effects on host drought resistance. Key findings include: (1) Rhizosphere microbial network connectivity decreased by 63.3% at 3 drought cycles (R3) versus control (R0), while bulk soil only decreased by 11.6%, showing niche-specific adaptation; (2) fungal communities responded rapidly to short-term drought stress, while bacterial (e.g., Proteobacteria) taxa exhibited delayed yet specific recruitment patterns across successive drought cycles, suggesting a time-resolved functional synergy; (3) transplanting R3 rhizosphere soil increased L. chinensis the content of relative water, proline, chlorophyll and soluble sugar, while reducing the relative conductivity and malondialdehyde content, validating the microbial-mediated 'stress memory' effect. These findings reveal that L. chinensis enhances drought adaptation by targeting the recruitment of rhizosphere microbes, providing valuable insights into the ecological resilience and restoration of grasslands.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Multiscale Imaging and Noninvasive Micro-Sensing Decipher Spatiotemporal Calcium Dynamics in Thermogenic Tissue of Magnolia Flower. 综合多尺度成像与无创微传感解译玉兰产热组织钙的时空动态。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-23 DOI: 10.1111/pce.70196

Siqin Wang, Miao Yu, Zhang Wang, Jiying Li, Chang Liu, Dongye Liu, Jing Li, Ruohan Wang

{"title":"Integrated Multiscale Imaging and Noninvasive Micro-Sensing Decipher Spatiotemporal Calcium Dynamics in Thermogenic Tissue of Magnolia Flower.","authors":"Siqin Wang, Miao Yu, Zhang Wang, Jiying Li, Chang Liu, Dongye Liu, Jing Li, Ruohan Wang","doi":"10.1111/pce.70196","DOIUrl":"https://doi.org/10.1111/pce.70196","url":null,"abstract":"Magnolia denudata is characterized by floral thermogenesis and blooms in the cold early spring. However, the specific thermogenic tissues and molecular signals that modulate thermogenesis remain elusive. Here, we categorized the developmental process of M. denudata into five stages with stage 2 being the thermogenic peak stage, and identified the thermogenic region as the lateral tissue of gynoecium by integrating infrared (IR) imaging and multispectral imaging (MSI). The optimized integration of these imaging techniques not only distinguished the gynoecium of the non-thermogenic and thermogenic stages but also revealed compound differences between the lateral and central tissues at the thermogenic stage. Moreover, we unveiled the in situ distribution of calcium in thermogenic organs using micro X-ray fluorescence imaging (μ-XRF), and its distribution pattern closely matched the heat distribution. The increased rate of Ca2+ influx both into the cytosol and mitochondria aligns with the upregulation of genes related to mitochondrial Ca2+ transport at the thermogenic stage. Additionally, changes in respiratory capacity caused by altering cytosolic Ca2+ concentration further demonstrated that Ca2+ regulates mitochondrial respiratory metabolism. This study comprehensively utilized multiscale imaging to distinguish the thermogenic tissue within the complex-structured thermogenic organ of M. denudata, revealing the close relationship between Ca2+ and thermogenesis.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Climate and Soil Factors Shape Microbial Diversity and Key Taxa Influencing Poplar Growth in Plantations. 气候和土壤因子塑造了影响人工林杨树生长的微生物多样性和关键类群。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-23 DOI: 10.1111/pce.70197

Mingzhen Xu, Qiqi Yang, Xiaolei Shen, Dingyi Yu, Zelin Zhao, Zimo Wang, Jinghan Wang, Pingdong Zhang, Changjun Ding, Changxiong Zhu, Hui Guo, Yinglong Chen

{"title":"Climate and Soil Factors Shape Microbial Diversity and Key Taxa Influencing Poplar Growth in Plantations.","authors":"Mingzhen Xu, Qiqi Yang, Xiaolei Shen, Dingyi Yu, Zelin Zhao, Zimo Wang, Jinghan Wang, Pingdong Zhang, Changjun Ding, Changxiong Zhu, Hui Guo, Yinglong Chen","doi":"10.1111/pce.70197","DOIUrl":"https://doi.org/10.1111/pce.70197","url":null,"abstract":"In the poplar plantations, soil microorganisms-particularly key microbial taxa-can have a profound impact on plant growth through their responses to environmental changes. However, there remains a lack of understanding of how environmental factors drive microbial diversity and key taxa, and how these, in turn, affect poplar growth. This study investigated 6-year-old poplar plantations across three regions of the North China Plain (Beijing, Hebei, and Henan). Combined with high-throughput cultivation and pot experiments, the growth-promoting effects of culturable key microbial strains on poplars were assessed. The results showed significant differences in climate indices, poplar growth traits, soil physicochemical properties, and soil microbial communities among the plantation regions (p < 0.05). A synthetic community comprising key strains (Acinetobacter-Aa20, Bacillus-Aa15, Bacillus-Ba9, and Bacillus-Ab26) significantly promoted poplar seedling growth. The results indicated that mean annual precipitation and mean annual temperature influence soil nutrient availability, thereby affecting poplar growth. Key taxa may indirectly promote poplar growth by being recruited through soil nutrient dynamics. This study provides important ecological insights into the interaction mechanisms among environmental factors, plants, soil, and microorganisms in poplar plantation ecosystems.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhabdovirus Glycoprotein Triggers Janus Kinase/Signal Transducer and Activator of Transcription Antiviral Immunity via Direct Recognition by Insect Dome Receptor. 横纹肌病毒糖蛋白通过昆虫圆顶受体直接识别触发Janus激酶/信号转导和转录激活因子的抗病毒免疫。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-23 DOI: 10.1111/pce.70198

Tingting Yu, You Li, Mengjia Xu, Zhihao Wan, Dongsheng Jia, Taiyun Wei

{"title":"Rhabdovirus Glycoprotein Triggers Janus Kinase/Signal Transducer and Activator of Transcription Antiviral Immunity via Direct Recognition by Insect Dome Receptor.","authors":"Tingting Yu, You Li, Mengjia Xu, Zhihao Wan, Dongsheng Jia, Taiyun Wei","doi":"10.1111/pce.70198","DOIUrl":"https://doi.org/10.1111/pce.70198","url":null,"abstract":"Janus kinase/signal transducer and activator of transcription (JAK/STAT) immune response traditionally requires cytokine binding to receptors for activation; however, it remains unclear whether cytokine receptors function as direct pattern recognition receptors (PRRs) that recognise viral envelopes. In this study, we demonstrate that cytokine receptor Domeless (Dome) in leafhopper Recilia dorsalis directly recognises glycoprotein (G) of an important rice rhabdovirus. This binding induces Dome dimerisation, which initiates JAK/STAT pathway and leads to STAT-dependent expression of antiviral immune effector in R. dorsalis (RdIE1). RdIE1 suppresses viral replication by inhibiting the RNA-binding activity of viral large polymerase (L). Dome residue T659 is essential for G recognition and L residues E774 and D775 are required for RdIE1 target. Notably, virus-encoded phosphoprotein competitively inhibits the phosphorylation of STAT by casein kinase II (CK2β), thereby abrogating STAT-dependent transactivation of target genes. Our findings redefine Dome as a viral sensor and reveal a biphasic strategy that balances antiviral immunity with persistent viral infection in insect vectors.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Elevated CO₂ on Bean Pod Mottle Virus Infection in Both Incompatible and Compatible Interactions With Phaseolus vulgaris L. CO2升高对豆荚斑驳病毒与菜豆不亲和亲和互作感染的影响

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-23 DOI: 10.1111/pce.70191

Tiffanie Scandolera, Julie Lintz, Ariane Gratias, Ellea Aboud, Simon Rochoux, Maria V Préjean, Gilles Chatel Innocenti, Marie Garmier, Gianluca Teano, Juan C Alvarez-Diaz, Graham Noctor, Valérie Geffroy, Stéphanie Pflieger

{"title":"Effects of Elevated CO2 on Bean Pod Mottle Virus Infection in Both Incompatible and Compatible Interactions With Phaseolus vulgaris L.","authors":"Tiffanie Scandolera, Julie Lintz, Ariane Gratias, Ellea Aboud, Simon Rochoux, Maria V Préjean, Gilles Chatel Innocenti, Marie Garmier, Gianluca Teano, Juan C Alvarez-Diaz, Graham Noctor, Valérie Geffroy, Stéphanie Pflieger","doi":"10.1111/pce.70191","DOIUrl":"https://doi.org/10.1111/pce.70191","url":null,"abstract":"Plant viruses cause significant crop losses, a situation that could worsen due to anthropogenic activities driving global climate change, one factor of which is the increase in atmospheric CO2 concentration. This study assessed the impact of elevated CO2 concentration (eCO2, 1000 vs. 400 ppm) on two genotypes of common bean (Phaseolus vulgaris L.), one susceptible and one resistant, infected with bean pod mottle virus (BPMV, Comovirus siliquae). For both genotypes, we found that plant growth, development and physiology were not enhanced under eCO2 enrichment in healthy plants, at the stage of BPMV inoculation. Under eCO2, the number of primary infection sites was reduced in both genotypes. Consistently, viral titre in inoculated leaves was lower, suggesting an enhanced resistance to BPMV in both genotypes under eCO2. To investigate the underlying mechanisms, we studied the expression of genes involved in different antiviral immune pathways: salicylic acid (SA)-signalling, RNA silencing and PAMP-triggered immunity (PTI) pathways. Under our experimental conditions, eCO2 neither primed the SA-signalling pathway nor the PTI pathway, in both genotypes. However, eCO2 seems to prime the RNA silencing pathway in the resistant genotype, and to a lesser extent, in the susceptible genotype.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemically and Microbially Mediated Kinship Strategies in Rice Cultivar Mixtures. 水稻杂交品种中化学和微生物介导的亲缘关系策略。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-23 DOI: 10.1111/pce.70202

Xin Chen, You Xu, Guo-Chun Ding, Scott J Meiners, Chui-Hua Kong

{"title":"Chemically and Microbially Mediated Kinship Strategies in Rice Cultivar Mixtures.","authors":"Xin Chen, You Xu, Guo-Chun Ding, Scott J Meiners, Chui-Hua Kong","doi":"10.1111/pce.70202","DOIUrl":"https://doi.org/10.1111/pce.70202","url":null,"abstract":"Improved yields can occur in closely related cultivar mixtures. Despite increasing knowledge of the benefits of neighbour-relatedness in intraspecific interactions, little is known about chemically and microbially mediated kinship responses in cultivar mixtures. Using a series of field and controlled experiments that used two sets of rice cultivars of varying genetic relatedness, we demonstrated that increased yield in rice cultivar mixtures was mediated by a root signaling chemical and soil microbes in a relatedness-dependent manner. Focal rice cultivars could discriminate closely from distantly related cultivars responded by altering root behavior, biomass allocation, and flowering time, improving grain yield. Relatedness discrimination was accompanied by an alteration in root signaling (-)-loliolide and, subsequently, the soil microbial community. Furthermore, (-)-loliolide directly modified soil microbes that were related to flowering time and seed biomass. Therefore, neighbor relatedness shapes soil microbial communities, generating kinship effects in rice cultivar mixtures. In particular, root signaling (-)-loliolide levels and soil microbial responses generated improvement of grain yield in closely related rice cultivar mixtures. The findings not only yield critical insights into plant-neighbor and plant-soil interactions but also raise an intriguing possibility to increase crop production by manipulating kinship in cultivar mixtures.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimating the Photorespiratory CO₂ Compensation Point and CO₂ Release in the Light Using the Laisk Method Combined With Photosynthetic Theory. 利用Laisk法结合光合理论估算光呼吸CO2补偿点和光释放CO2。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-23 DOI: 10.1111/pce.70195

Darwin L Moreno-Echeverry, Miko U F Kirschbaum, Margaret M Barbour, Lìyǐn L Liáng

{"title":"Estimating the Photorespiratory CO2 Compensation Point and CO2 Release in the Light Using the Laisk Method Combined With Photosynthetic Theory.","authors":"Darwin L Moreno-Echeverry, Miko U F Kirschbaum, Margaret M Barbour, Lìyǐn L Liáng","doi":"10.1111/pce.70195","DOIUrl":"https://doi.org/10.1111/pce.70195","url":null,"abstract":"The photorespiratory CO2 compensation point (Γ*) and the rate of CO2 release in the light (DL) are critical parameters for understanding the carbon dynamics of C3 plants. These two parameters can be derived from the widely-used Laisk method as the intercept of linear regression lines fitted to net assimilation rates (Anet) vs. chloroplast CO2 partial pressures (Cc) obtained at different low-irradiance levels. However, photosynthetic theory indicates curvature in the Anet-Cc relationship which conflicts with the use of linear relationships for analysis. We, therefore, systematically evaluated the limitations of the use of linear relationships across temperatures from 5°C to 40°C and quantified the sensitivity of errors in Γ* and DL estimates to the selected Cc range. We found that wide CO2 ranges, especially when they exclude the expected Γ*, can introduce substantial biases in parameter estimation with linear regressions, particularly at lower temperatures. This can lead to marked underestimates of Γ*, and biologically unrealistic DL. We propose refining the Laisk method by using a photosynthesis model to analyse data. The model better represents the nonlinear Anet-Cc relationship and yields consistent Γ* and DL estimates, regardless of the CO2 range used.","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signalling Networks Underlying Cell Wall Responses to Salinity Stress. 盐胁迫下细胞壁反应的信号网络。

IF 6.3 1区生物学

Plant, Cell & Environment Pub Date : 2025-09-18 DOI: 10.1111/pce.70194

George Bawa, Ruiwen Kong, Xi Chen, Jagna Chmielowska-Bąk, Weibing Yang, Xiaoli Sun, Mingzhe Sun

引用次数: 0