高温通过代谢和氧化失调破坏玉米丝的功能。

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Mayang Liu, Yingda Huang, Zheng Li, Xuanlong Lv, Mingqi Gu, Shuhua Liao, Xin Dong, Yingbo Gao, Zhen Gao, Pu Wang, Shoubing Huang
{"title":"高温通过代谢和氧化失调破坏玉米丝的功能。","authors":"Mayang Liu, Yingda Huang, Zheng Li, Xuanlong Lv, Mingqi Gu, Shuhua Liao, Xin Dong, Yingbo Gao, Zhen Gao, Pu Wang, Shoubing Huang","doi":"10.1111/pce.70201","DOIUrl":null,"url":null,"abstract":"<p><p>High temperature (HT, ≥ 38°C) impairs maize (Zea mays L.) yield by disrupting pollination, yet mechanisms in female reproductive organs remain elusive. Maize silks, the essential tissues for pollen capture and pollen tube growth, are particularly sensitive to HT, are highly vulnerable to HT. Here, we combined phenotypic, physiological, metabolic and transcriptomic analyses under controlled HT (40/30°C) and control (32/22°C) conditions to dissect mechanisms underlying HT-induced silk growth inhibition (SGI) and silk pollination dysfunction (SPD). HT reduced silk emergence by ~20% but decreased seed set by ~50%, indicating SPD dominated kernel loss over SGI. HT significantly downregulated key genes of the silks that encode sucrose transporters, sugars will eventually be exported through transporters and glycolytic enzymes (hexokinase; 6-phosphofructokinase; pyruvate kinase), restricting energy metabolism required for silk elongation and pollen tube growth. Concurrently, HT elevated abscisic acid and indole-3-acetic acid while suppressing zeatin riboside, brassinolide and jasmonic acid levels, collectively driving SGI. SPD was primarily linked to oxidative damage via suppressed flavonoid biosynthesis (chalcone synthase, flavonol synthase and peroxidase) and impaired reactive oxygen species (ROS) scavenging. Specifically, HT induced a negative correlation between ZmARF1 and ZmSOD3 expression, suggesting compromised ROS clearance that exacerbated silk structural damage. These findings provide new insights into the metabolic, hormonal and transcriptional regulatory networks that govern silk thermotolerance, providing potential molecular targets for breeding heat-resilient maize varieties.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3000,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"High Temperature Disrupts Maize Silk Function Through Metabolic and Oxidative Dysregulation.\",\"authors\":\"Mayang Liu, Yingda Huang, Zheng Li, Xuanlong Lv, Mingqi Gu, Shuhua Liao, Xin Dong, Yingbo Gao, Zhen Gao, Pu Wang, Shoubing Huang\",\"doi\":\"10.1111/pce.70201\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>High temperature (HT, ≥ 38°C) impairs maize (Zea mays L.) yield by disrupting pollination, yet mechanisms in female reproductive organs remain elusive. Maize silks, the essential tissues for pollen capture and pollen tube growth, are particularly sensitive to HT, are highly vulnerable to HT. Here, we combined phenotypic, physiological, metabolic and transcriptomic analyses under controlled HT (40/30°C) and control (32/22°C) conditions to dissect mechanisms underlying HT-induced silk growth inhibition (SGI) and silk pollination dysfunction (SPD). HT reduced silk emergence by ~20% but decreased seed set by ~50%, indicating SPD dominated kernel loss over SGI. HT significantly downregulated key genes of the silks that encode sucrose transporters, sugars will eventually be exported through transporters and glycolytic enzymes (hexokinase; 6-phosphofructokinase; pyruvate kinase), restricting energy metabolism required for silk elongation and pollen tube growth. Concurrently, HT elevated abscisic acid and indole-3-acetic acid while suppressing zeatin riboside, brassinolide and jasmonic acid levels, collectively driving SGI. SPD was primarily linked to oxidative damage via suppressed flavonoid biosynthesis (chalcone synthase, flavonol synthase and peroxidase) and impaired reactive oxygen species (ROS) scavenging. Specifically, HT induced a negative correlation between ZmARF1 and ZmSOD3 expression, suggesting compromised ROS clearance that exacerbated silk structural damage. These findings provide new insights into the metabolic, hormonal and transcriptional regulatory networks that govern silk thermotolerance, providing potential molecular targets for breeding heat-resilient maize varieties.</p>\",\"PeriodicalId\":222,\"journal\":{\"name\":\"Plant, Cell & Environment\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":6.3000,\"publicationDate\":\"2025-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant, Cell & Environment\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://doi.org/10.1111/pce.70201\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant, Cell & Environment","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/pce.70201","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

高温(HT,≥38°C)通过破坏传粉影响玉米(Zea mays L.)产量,但其对雌性生殖器官的影响机制尚不清楚。玉米丝作为花粉捕获和花粉管生长的重要组织,对高温特别敏感,极易受到高温胁迫。在这里,我们结合表型、生理、代谢和转录组学分析,在控制高温(40/30°C)和控制高温(32/22°C)条件下,剖析高温诱导蚕丝生长抑制(SGI)和蚕丝授粉功能障碍(SPD)的机制。高温处理使蚕丝出苗率降低了约20%,但使结实率降低了约50%,表明高温处理对SGI的籽粒损失起主导作用。高温显著下调了蚕丝中编码蔗糖转运蛋白的关键基因,糖最终会通过转运蛋白和糖酵解酶(己糖激酶、6-磷酸果糖激酶、丙酮酸激酶)输出,限制了蚕丝伸长和花粉管生长所需的能量代谢。同时,HT升高脱落酸和吲哚-3-乙酸,同时抑制玉米素核苷、油菜素内酯和茉莉酸水平,共同驱动SGI。SPD主要通过抑制类黄酮生物合成(查尔酮合成酶、黄酮醇合成酶和过氧化物酶)和抑制活性氧(ROS)清除与氧化损伤有关。具体而言,高温诱导ZmARF1和ZmSOD3表达负相关,表明ROS清除受损加剧了丝结构损伤。这些发现为研究蚕丝耐热性的代谢、激素和转录调控网络提供了新的见解,为培育耐热玉米品种提供了潜在的分子靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High Temperature Disrupts Maize Silk Function Through Metabolic and Oxidative Dysregulation.

High temperature (HT, ≥ 38°C) impairs maize (Zea mays L.) yield by disrupting pollination, yet mechanisms in female reproductive organs remain elusive. Maize silks, the essential tissues for pollen capture and pollen tube growth, are particularly sensitive to HT, are highly vulnerable to HT. Here, we combined phenotypic, physiological, metabolic and transcriptomic analyses under controlled HT (40/30°C) and control (32/22°C) conditions to dissect mechanisms underlying HT-induced silk growth inhibition (SGI) and silk pollination dysfunction (SPD). HT reduced silk emergence by ~20% but decreased seed set by ~50%, indicating SPD dominated kernel loss over SGI. HT significantly downregulated key genes of the silks that encode sucrose transporters, sugars will eventually be exported through transporters and glycolytic enzymes (hexokinase; 6-phosphofructokinase; pyruvate kinase), restricting energy metabolism required for silk elongation and pollen tube growth. Concurrently, HT elevated abscisic acid and indole-3-acetic acid while suppressing zeatin riboside, brassinolide and jasmonic acid levels, collectively driving SGI. SPD was primarily linked to oxidative damage via suppressed flavonoid biosynthesis (chalcone synthase, flavonol synthase and peroxidase) and impaired reactive oxygen species (ROS) scavenging. Specifically, HT induced a negative correlation between ZmARF1 and ZmSOD3 expression, suggesting compromised ROS clearance that exacerbated silk structural damage. These findings provide new insights into the metabolic, hormonal and transcriptional regulatory networks that govern silk thermotolerance, providing potential molecular targets for breeding heat-resilient maize varieties.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信