{"title":"PlOBP1/PlDAM-PlSOC1 Module Regulates Bud Dormancy Transition in Response to Low Temperature.","authors":"Xiaobin Wang, Xiaoxuan Chen, Kaijing Zhang, Danqing Li, Lingmei Shao, Tong Xu, Ziming Ren, Qiyao Wang, Junhong Guo, Runlong Zhang, Cong Gao, David P Horvath, Yiping Xia, Jiaping Zhang","doi":"10.1111/pce.70218","DOIUrl":null,"url":null,"abstract":"<p><p>The transition from bud endodormancy to ecodormancy is triggered by environmental cues, particularly low temperatures. However, the mechanism underlying bud dormancy transition (BDT) is largely unknown. Here, we identified a low-temperature-responsive, MADS-box family SUPPRESSOR OF OVEREXPRESSION OF CO1 (PlSOC1) under both natural and controlled low temperatures, which promotes BDT and confers low chilling requirement trait of herbaceous peony (Paeonia lactiflora). A novel transcription factor, DNA-binding One Zinc Finger (DOF) family OBF BINDING PROTEIN 1 (PlOBP1), was found to bind the AAAAG motif in the PlSOC1 promoter, acting as a negative regulator of BDT. PlOBP1 acts together with Dormancy Associated MADS-box (PlDAM) protein to enhance the transinhibitory effect of PlSOC1. Further gibberellic acid (GA) treatment showed that exogenous GA can replace long-term chilling to promote BDT, likely by inhibiting the expression of PlOBP1 while inducing the expression of PlSOC1. The elevated PlSOC1 forms a complex with PlDAM and inhibits PlDAM activity, further releasing the inhibition on PlSOC1, thereby amplifying PlSOC1 and triggering BDT. Our findings provide mechanistic insights into low-temperature-mediated GA regulation of BDT and reveal a novel role of DOF protein PlOBP1 and its interactions with MADS-box family members in bud dormancy regulation.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant, Cell & Environment","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/pce.70218","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The transition from bud endodormancy to ecodormancy is triggered by environmental cues, particularly low temperatures. However, the mechanism underlying bud dormancy transition (BDT) is largely unknown. Here, we identified a low-temperature-responsive, MADS-box family SUPPRESSOR OF OVEREXPRESSION OF CO1 (PlSOC1) under both natural and controlled low temperatures, which promotes BDT and confers low chilling requirement trait of herbaceous peony (Paeonia lactiflora). A novel transcription factor, DNA-binding One Zinc Finger (DOF) family OBF BINDING PROTEIN 1 (PlOBP1), was found to bind the AAAAG motif in the PlSOC1 promoter, acting as a negative regulator of BDT. PlOBP1 acts together with Dormancy Associated MADS-box (PlDAM) protein to enhance the transinhibitory effect of PlSOC1. Further gibberellic acid (GA) treatment showed that exogenous GA can replace long-term chilling to promote BDT, likely by inhibiting the expression of PlOBP1 while inducing the expression of PlSOC1. The elevated PlSOC1 forms a complex with PlDAM and inhibits PlDAM activity, further releasing the inhibition on PlSOC1, thereby amplifying PlSOC1 and triggering BDT. Our findings provide mechanistic insights into low-temperature-mediated GA regulation of BDT and reveal a novel role of DOF protein PlOBP1 and its interactions with MADS-box family members in bud dormancy regulation.
期刊介绍:
Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.