Plant-Mediated RNAi of Non-ATPase Regulatory Subunit Confers Soybean Resistance Against Bean Bug, Riptortus pedestris.

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Manru Xu, Yuanyu Zhang, Jin Zhao, Xiangdong Yang, Huajuan Li, Biao Hu, Jianping Chen, Zongtao Sun, Zhongyan Wei
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引用次数: 0

Abstract

Riptortus pedestris, is a major pest threatening soybeans and cause the soybean staygreen syndrome, which has recently become a significant and widespread issue in soybean production. Currently, chemical control remains the primary method for managing R. pedestris in the field, but it contributes to environmental pollution and increases the resistance risk. Plant-mediated RNA interference (RNAi) offers a target-specific and eco-friendly alternative for pest control. While RNAi has been shown to effectively control certain Coleoptera, its effects on piercing-sucking insects, such as R. pedestris, remain poorly understood. In this study, we characterized a non-ATPase regulatory subunit 6 (RPN6) from R. pedestris and demonstrated that downregulation of RPN6 expression significantly increased mortality and reduced oviposition. Phylogenetic analysis revealed that while RPN6 is highly conserved across Hemiptera, the double-stranded RNA (dsRNA) targeting RPN6 is species-specific. We subsequently developed dsRPN6-transgenic soybean lines, and feeding bioassays revealed these transgenic soybean lines exhibited high resistance to R. pedestris, with significantly reduced survival and oviposition rates in the pest. Importantly, the transgenic soybeans displayed moderate staygreen symptoms following infestation by R. pedestris, demonstrating that plant-mediated RNAi targeting of RPN6 confers effective resistance against R. pedestris, providing a promising strategy for bean bug management in agricultural practices.

植物介导的非三磷酸腺苷酶调控亚基的RNAi赋予大豆对豆虫的抗性。
梨蛾是危害大豆的主要害虫,是造成大豆滞绿综合征的主要原因,近年来已成为大豆生产中一个重要而普遍的问题。目前,化学防治仍然是r . pedestris总经理的主要方法,但它会导致环境污染和增加抵抗风险。植物介导的RNA干扰(RNAi)为害虫防治提供了一种目标特异性和生态友好的替代方法。虽然RNAi已被证明可以有效地控制某些鞘翅目昆虫,但它对刺吸昆虫的影响,如r.s testris,仍然知之甚少。在这项研究中,我们鉴定了一个非atp酶调控亚基6 (RPN6),并证明了RPN6表达下调会显著增加死亡率和减少产卵。系统发育分析表明,虽然RPN6在半翅目中高度保守,但靶向RPN6的双链RNA (dsRNA)具有物种特异性。随后,我们培育了dsrpn6转基因大豆品系,并通过饲养生物测定发现,这些转基因大豆品系对黄颡鱼蛾具有较高的抗性,显著降低了黄颡鱼蛾的存活率和产卵率。重要的是,转基因大豆在被黄粉虫侵染后表现出中度的滞绿症状,这表明植物介导的RNAi靶向rn6对黄粉虫具有有效的抗性,为农业实践中的豆虫管理提供了一种有前景的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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