PlOBP1/PlDAM-PlSOC1模块调控低温下芽休眠转变

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Xiaobin Wang, Xiaoxuan Chen, Kaijing Zhang, Danqing Li, Lingmei Shao, Tong Xu, Ziming Ren, Qiyao Wang, Junhong Guo, Runlong Zhang, Cong Gao, David P Horvath, Yiping Xia, Jiaping Zhang
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引用次数: 0

摘要

从芽内休眠到生态休眠的转变是由环境因素触发的,尤其是低温。然而,芽休眠转变(BDT)的机制在很大程度上是未知的。本研究鉴定了一个低温响应型MADS-box家族CO1过表达抑制子(PlSOC1),该抑制子在自然低温和受控低温下均能促进芍药(Paeonia lactiflora)的BDT,并赋予其低冷性特性。一种新的转录因子,dna结合One Zinc Finger (DOF) family OBF BINDING PROTEIN 1 (PlOBP1),被发现结合PlSOC1启动子中的AAAAG基序,作为BDT的负调控因子。PlOBP1与休眠相关MADS-box (PlDAM)蛋白共同作用,增强PlSOC1的转抑制作用。进一步的赤霉素(gibberellic acid, GA)处理表明,外源赤霉素(gibberellic acid, GA)可能通过抑制PlOBP1的表达而诱导PlSOC1的表达,从而代替长时间的低温处理来促进BDT。升高的PlSOC1与PlDAM形成复合物,抑制PlDAM活性,进一步释放对PlSOC1的抑制,从而放大PlSOC1,触发BDT。我们的研究结果为低温介导的GA调控BDT提供了机制见解,并揭示了DOF蛋白PlOBP1及其与MADS-box家族成员在芽休眠调控中的新作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PlOBP1/PlDAM-PlSOC1 Module Regulates Bud Dormancy Transition in Response to Low Temperature.

The transition from bud endodormancy to ecodormancy is triggered by environmental cues, particularly low temperatures. However, the mechanism underlying bud dormancy transition (BDT) is largely unknown. Here, we identified a low-temperature-responsive, MADS-box family SUPPRESSOR OF OVEREXPRESSION OF CO1 (PlSOC1) under both natural and controlled low temperatures, which promotes BDT and confers low chilling requirement trait of herbaceous peony (Paeonia lactiflora). A novel transcription factor, DNA-binding One Zinc Finger (DOF) family OBF BINDING PROTEIN 1 (PlOBP1), was found to bind the AAAAG motif in the PlSOC1 promoter, acting as a negative regulator of BDT. PlOBP1 acts together with Dormancy Associated MADS-box (PlDAM) protein to enhance the transinhibitory effect of PlSOC1. Further gibberellic acid (GA) treatment showed that exogenous GA can replace long-term chilling to promote BDT, likely by inhibiting the expression of PlOBP1 while inducing the expression of PlSOC1. The elevated PlSOC1 forms a complex with PlDAM and inhibits PlDAM activity, further releasing the inhibition on PlSOC1, thereby amplifying PlSOC1 and triggering BDT. Our findings provide mechanistic insights into low-temperature-mediated GA regulation of BDT and reveal a novel role of DOF protein PlOBP1 and its interactions with MADS-box family members in bud dormancy regulation.

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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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