生命科学最新文献

{"title":"Transcription Factors Regulating Nutrient and Ion Uptake in Plants Exposed to Abiotic and Biotic Stress Conditions.","authors":"Krishna Kumar Rai, Francisco J Corpas","doi":"10.1111/pce.70779","DOIUrl":"10.1111/pce.70779","url":null,"abstract":"<p><p>Plant growth and productivity are severely constrained by abiotic and biotic stresses, which disrupt essential physiological processes, particularly nutrient and ion homeostasis. In response to these challenges, plants activate sophisticated molecular networks, with transcription factors (TFs) emerging as central regulators that integrate stress signals with nutrient management. This review synthesizes current knowledge on the pivotal role of TFs in coordinating stress resilience and nutrient acquisition. It elaborates on how distinct TF families, including AP2/ERF, NAC, MYB, and bZIP, are regulated through complex signal transduction pathways involving reactive oxygen species (ROS), phytohormones, and second messengers. Upon activation, these TFs directly modulate the expression of genes encoding nutrient transporters, ion channels, and detoxification proteins, thereby maintaining cellular ion balance and facilitating nutrient uptake under stress. Notably, specific TFs such as NLP7, HY5, and STOP1 are highlighted for their direct role in regulating the homeostasis of key nutrients like nitrate, phosphate, and metals, linking nutrient signaling to adaptive responses. On the other hand, key TF families, including WRKY, MYB, bZIP, AP2/ERF, and NAC, have also been studied for their role in biotic stress tolerance. Despite these advances, the identification of TFs that specifically govern nutrient transport under combined stresses remains limited. We conclude that advanced functional genomics, machine learning, and genetic engineering to manipulate these master regulatory networks present a transformative strategy for developing next-generation crops with enhanced nutrient use efficiency and climate resilience, which is critical for achieving global food security.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"8712-8734"},"PeriodicalIF":6.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148597063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-Myristoylation in Plants: Molecular Mechanisms, Protein Fate, and Roles in Stress Responses and Development.","authors":"Linghui Chen, Yue Zhang, Yi Man, Xiaoxin Zhang, Yanping Jing","doi":"10.1111/pce.70771","DOIUrl":"10.1111/pce.70771","url":null,"abstract":"<p><p>N-myristoylation is a common yet poorly understood protein lipid modification in all eukaryotes, including plants. It involves the covalent attachment of myristic acid to the N-terminal glycine of target proteins, catalyzed by N-myristoyltransferase. This modification can alter the structure and membrane anchoring of target proteins, thereby playing a significant role in regulating protein fate, including subcellular compartmentalization and stability. Currently, increasing evidence has highlighted the vital role of N-myristoylation in regulating plant immunity, abiotic stress responses, growth, and development, underscoring its biological significance for plant environmental adaptation and survival. Despite its importance, the fundamental mechanisms of N-myristoylation and its specific regulatory effects on plant protein function and fate remain incompletely characterized. In this review, we first summarize the core biochemical process of protein N-myristoylation and the functional features of its catalytic enzyme, N-myristoyltransferase. We then elaborate on the molecular basis by which this modification modulates protein fate, before providing a comprehensive overview of its diverse biological functions in the aforementioned plant physiological processes. Additionally, we critically evaluate major technical approaches for detecting N-myristoylation, clarifying their respective advantages and inherent limitations. Finally, we propose promising future directions, aiming to provide a theoretical foundation for subsequent investigations in this rapidly developing field.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"8611-8625"},"PeriodicalIF":6.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsERF104: A Transcriptional Gatekeeper Linking Ethylene Signalling to Cadmium Detoxification in Rice.","authors":"Subhasis Karmakar, Sabarinathan Selvaraj, Subhankar Mondal, Satyaki Biswas, Dipak Gayen","doi":"10.1111/pce.70765","DOIUrl":"10.1111/pce.70765","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"8604-8606"},"PeriodicalIF":6.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CaSnRK2.4-Mediated Phosphorylation of CabZIP032 Regulates CaABI5 and CaSPS1 Expression to Modulate ABA Signalling and Sucrose Metabolism During Drought Tolerance in Pepper.","authors":"Huafeng Zhang, Yingping Pei, Jing Zhong, Bolin Zhao, Ikram Ullah, Rugang Chen","doi":"10.1111/pce.70784","DOIUrl":"10.1111/pce.70784","url":null,"abstract":"<p><p>Drought stress severely impacts plant growth and productivity; however, the molecular mechanisms underlying stress adaptation in non-model crops remain incompletely understood. In this study, we discovered that the SnRK2 kinase CaSnRK2.4 and the bZIP transcription factor CabZIP032 positively regulate drought tolerance through ABA and sucrose signalling pathways. Yeast two-hybrid and in planta assays confirmed direct interaction between CaSnRK2.4 and CabZIP032, with CaSnRK2.4 phosphorylating CabZIP032 at serine 251 (Ser251). Phosphorylation-defective CabZIP032^S251A mutants exhibited protein stability and compromised drought tolerance, whereas phosphomimetic CabZIP032^S251D mutants showed enhanced stress resilience. Functional analyses revealed that CabZIP032 binds to the promoters of CaABI5, an ABA-responsive gene, and CaSPS1, a sucrose biosynthesis gene, activating their transcription via specific recognition of ACGT and G-box cis-elements, respectively. Silencing of CaABI5 or CaSPS1 results in increased drought sensitivity and reduced ABA and soluble sugar levels. Notably, exogenous applications of ABA or sucrose rescued drought tolerance in CabZIP032-silenced plants, while phosphorylation at Ser251 further potentiated CabZIP032 transcriptional activity. Collectively, these findings establish a CaSnRK2.4-CabZIP032 signalling module that orchestrates drought adaptation by stabilising CabZIP032 through phosphorylation, thereby promoting ABA signalling and sucrose accumulation. This study reveals a conserved regulatory mechanism in pepper and provides potential molecular targets for engineering drought-resilient crops.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"8779-8798"},"PeriodicalIF":6.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatin Remodelling Complexes in Plant Adaptation to Abiotic Stress: Mechanisms and Perspectives.","authors":"Xiuxiu Zhang, Yueran Zhang, Jiang Li","doi":"10.1111/pce.70794","DOIUrl":"10.1111/pce.70794","url":null,"abstract":"<p><p>As sessile organisms, plants have evolved elaborate epigenetic regulatory networks to adapt to a wide range of abiotic stress. Chromatin remodelling is a core epigenetic regulatory mechanism that refers to the process by which chromatin remodelling complexes mediate dynamic changes in chromatin spatial conformation and DNA accessibility through nucleosome sliding, histone variant exchange, nucleosome eviction, or spacing adjustment. The flexible and reversible modifications of chromatin structure enable plants to rapidly respond to external stimuli, thereby safeguarding their survival and promoting adaptive plasticity. This review summarises the research progress on chromatin remodelling complexes and their regulatory mechanisms in plant environmental adaptation. Furthermore, we discuss the current challenges and future research directions in this field, including the need to decipher the recruitment specificity of chromatin remodellers, investigate their coordination networks under combined abiotic stresses, and translate mechanistic insights into epigenetic engineering for crop improvement. Collectively, this review aims to provide a comprehensive overview of chromatin remodelling-mediated stress adaptation and lay a foundation for developing stress-tolerant crop varieties via epigenetic engineering.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"9117-9132"},"PeriodicalIF":6.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13646677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148704940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concurrent Increases in Root Phosphorus Acquisition and Leaf Phosphorus Resorption Underpin Enhanced Phosphorus Economy in Chickpea During Reproductive Development.","authors":"Xiaolong Feng, Shuyan Li, Jiayin Pang, Peta L Clode, Hans Lambers, Kadambot H M Siddique","doi":"10.1111/pce.70797","DOIUrl":"10.1111/pce.70797","url":null,"abstract":"<p><p>Phosphorus (P) acquisition and leaf P-use strategies are critical for reproductive success and productivity, yet, their developmental association during the reproductive phase remains unclear. Five chickpea accessions contrasting in P-remobilisation efficiency (PRE) were grown in a temperature-controlled glasshouse under moderate P deficiency. Root P-acquisition traits (rhizosheath carboxylates and rhizosheath acid phosphatase activity) and leaf P-use traits (PRE, photosynthetic P-use efficiency (PPUE), P fractions, and leaf acid phosphatase activity) were measured. Phosphorus acquisition substantially increased during reproduction compared with the vegetative stage, with greater carboxylates recovered from the rhizosheath and higher rhizosheath acid phosphatase activity. Root nitrogen (N) and P concentrations increased by 108% and 54%, respectively. Leaf PRE increased by 28%, with higher resorption efficiency across most P fractions, whereas N-resorption efficiency was stable. Although area-based photosynthetic rate increased by 34%, PPUE did not, and we observed no trade-off between PPUE and PRE. Leaf thickness and leaf mass per area increased by 22% and 34%, respectively, partially explaining the faster photosynthetic rate. Concurrent we also observed increases in transpiration, and this may be associated with increased photosynthetic capacity. These results reveal coordinated increases in root P acquisition and leaf P remobilisation during reproduction, likely driven by stronger sink demand.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"9085-9100"},"PeriodicalIF":6.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13646661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Transpiration and Whole-Tree Crown Conductance in Tropical Forests.","authors":"Mizanur Rahman, Alfonso Zambrano, Alistair Rogers, Shawn P Serbin, Kim S Ely, Kenneth J Davidson, Jean-Christophe Domec, Julien Lamour, Sean T Michaletz, Brett T Wolfe, Alexandria L Pivovaroff, Matteo Detto, Gustavo C Spanner, Jin Wu, Stuart J Davies, Jeffrey M Warren, Joseph Wright, Chonggang Xu, Zachary Robbins, Robinson Negron-Juarez, Ryan G Knox, Cynthia Wright, Jeffrey Q Chambers, Charles D Koven, Nate G McDowell","doi":"10.1111/pce.70719","DOIUrl":"10.1111/pce.70719","url":null,"abstract":"","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"8246-8249"},"PeriodicalIF":6.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13646684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Airborne Immunity: A Bacterial Volatile Primes Plant Defenses by Unlocking a Phytoalexin Biosynthetic Checkpoint.","authors":"Ivan A Paponov, Chidananda Nagamangala Kanchiswamy, Simone Bossi, Francesco Caldo, Ambra S Parmagnani, Mickael Malnoy, Massimo E Maffei","doi":"10.1111/pce.70816","DOIUrl":"10.1111/pce.70816","url":null,"abstract":"<p><p>Microbial volatile organic compounds (mVOCs) enable plants to perceive microbial activity prior to physical contact, yet the contribution of individual bacterial volatiles to immune signalling and disease resistance remains incompletely understood. Here, headspace GC-MS analysis demonstrates that the hemibiotrophic pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) emits a distinct volatile blend containing the bacterium-specific compounds 2-methylbutanoic acid (2-MBA) and 3-methylbutanoic acid (3-MBA), with 2MBA as a dominant component. Exposure of Arabidopsis thaliana to the complete Pst DC3000 mVOC blend induced extensive transcriptional reprogramming activation of pattern-recognition receptor-associated genes, MAP kinase signalling components, WRKY transcription factors, camalexin biosynthetic genes and early defense responses. The latter included cytosolic Ca²⁺ ([Ca²⁺]_cyt) elevation, K⁺ channel activation, hydrogen peroxide accumulation, and nitric oxide production, which culminated in camalexin accumulation in wild-type shoots and roots. In contrast, these early signaling and physiological responses were strongly attenuated in the camalexin-deficient pad3 mutant. Phenotypic priming assays confirmed that mVOC pre-exposure enhances resistance against subsequent Pst DC3000 infection largely through this PAD3-dependent mechanism, while exogenous camalexin administration proved independently sufficient to restore robust pathogen protection, indicating that PAD3-dependent camalexin biosynthesis contributes substantially, but not exclusively, to volatile-induced resistance. Application of synthetic 2-MBA, and to a lesser extent 3-MBA, was sufficient to recreate rapid [Ca²⁺]_cyt elevation and ROS production. Quantitative expression profiling revealed that synthetic 2-MBA modulates gene expression in an organ-specific manner, upregulating shoot volatile perception and salicylic acid pathways, inducing root calcium and immune responses, and systemically activating auxin signaling and tryptophan biosynthetic genes. Together, these findings identify 2-MBA as a primary active component of the Pst DC3000 volatile blend and indicate that full volatile-induced defence depends on PAD3-dependent camalexin accumulation together with integration of multiple volatile signals. Our results reveal how plants integrate distinct bacterial volatiles to trigger early signaling and coordinate localized and systemic camalexin-dependent immunity.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"9070-9084"},"PeriodicalIF":6.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13646668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere Engineering by Root Exudates: High-Yielding Alfalfa Recruits Functional PGPR to Sustain Soil Nutrient Availability Under Long-Term Cultivation.","authors":"Yanliang Sun, Kongqin Wei, Kaixin Yang, Jinfeng Hui, Dongjie Xia, Xuzhe Wang, Andrew D Cartmill, Ignacio F López, Chunhui Ma, Qianbing Zhang","doi":"10.1111/pce.70631","DOIUrl":"10.1111/pce.70631","url":null,"abstract":"<p><p>Soil nutrient transformation capacity is a critical determinant of sustainable productivity in perennial cropping systems; however, the extent to which high-yielding crops actively regulate rhizosphere microbial assembly to maintain nutrient availability remains poorly understood. We investigated whether root exudates from high-yielding alfalfa (Medicago sativa L.) selectively recruit plant growth-promoting rhizobacteria (PGPR) to enhance nutrient transformation. In an 8-year continuous alfalfa system (2018-2025), high-yielding cultivars increased soil organic carbon by 8.64%, total nitrogen by 6.01%, and moderately labile phosphorus fractions by 1.62%. Rhizobox experiments demonstrated that root exudates enhanced growth only with an active microbiome. High-yielding alfalfa enriched PGPR communities, specifically Ensifer, Pseudomonas, and Bacillus. Isolated strains exhibited N fixation, P solubilisation, and IAA production. Metabolomic profiling revealed that exudates were enriched in specific sugars and amino acids. Maltopentaose, maltotetraose, taurine, N-acetyl-L-leucine, and asparagine functioned as chemoattractants, stimulating PGPR proliferation and biofilm formation. These findings demonstrate that root exudate-mediated, targeted recruitment of functional PGPR enhances N fixation and P transformation, thereby supporting sustained high alfalfa productivity. This study demonstrates a key rhizosphere mechanism underlying the long-term sustainability of high-yielding perennial legume systems and provides a mechanistic basis for microbiome-informed sustainable alfalfa production and management.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":"8763-8778"},"PeriodicalIF":6.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13646654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide Signalling as a Control Layer for Signal Prioritisation in Plant Stress Networks.","authors":"Baber Ali, Peter Poczai, Nijat Imin","doi":"10.1111/pce.70976","DOIUrl":"https://doi.org/10.1111/pce.70976","url":null,"abstract":"<p><p>Plants in the field rarely encounter single stresses, and simultaneous stresses impose conflicting physiological demands that cannot be satisfied at once. Responses to such combinations are frequently non-additive and cannot be predicted from single-stress responses, a result that crosstalk models struggle to accommodate because they describe which pathways interact without specifying which prevails. This review proposes that peptide signalling functions as a control layer governing signal prioritisation. Under this hypothesis, secreted peptides act above the shared modules of cytosolic calcium, reactive oxygen species, and mitogen-activated protein kinase cascades, and determine which competing input is permitted to dominate their behaviour. Evidence for this proposal spans several peptide families. CEP, CLE and RALF signalling illustrate systemic demand signalling, developmental stabilisation, and receptor complex reorganisation respectively, and the same logic extends to PEP, IDA and PSK signalling across immune, developmental, and drought contexts. Combined drought and nitrogen limitation, resolved through ABA and CEP, provides a worked case in which a global signal sets operating boundaries within which a local signal refines the response, supported by evidence now extending from Arabidopsis into cereals. We derive falsifiable predictions tested against hormone-pathway negative controls and graded drought intensities rather than single treatments, which allows loss of prioritisation to be distinguished from general signalling fragility. We also identify a trade-off constraining crop application, since enhancing peptide-mediated nutrient foraging may reduce drought resilience. Substantial gaps remain in detecting peptide signals directly and in ranking peptide families under field conditions. Reframing plant signalling as a decision system rather than an input-output relay offers a testable foundation for improving stress resilience and nutrient use efficiency in crop breeding.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.9,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}