生命科学最新文献

{"title":"Characterization of FLOWERING LOCUS T-related genes and their putative gene regulatory network in semi-winter Brassica napus cultivar Zhongshaung11","authors":"Juanjuan Wang,&nbsp;Hao-Ran Zhou,&nbsp;Petra Tänzler,&nbsp;Na Ding,&nbsp;Jing Wang,&nbsp;Franziska Turck","doi":"10.1111/tpj.70443","DOIUrl":"https://doi.org/10.1111/tpj.70443","url":null,"abstract":"<p>In many species, <i>FLOWERING LOCUS T</i> (<i>FT</i>)-like genes promote the floral transition by integrating environmental signals, in particular photoperiod, and internal cues. Here we show that <i>Brassica napus</i> contains six <i>FT</i>-like genes and two pseudogenes belonging to three orthogroups. All <i>B. napus FT</i>-like genes induce early flowering when expressed at the shoot apical meristems of <i>Arabidopsis thaliana ft</i> mutants; however, <i>BnaFT.C6</i> and non-orthologous <i>FT</i>-like genes do not encode fully functional mobile florigens. In the case of <i>BnFT.C6</i>, the functional change is associated with a T to C amino acid change that is restricted to semi-winter accessions. Expression of orthologs of <i>FT</i> is photoperiod-dependent, and two distal enhancers are conserved; however, the homeologs <i>BnaFT.A7</i> and <i>BnaFT.C6</i> show rearrangements of DNA motifs binding NF-Y/CO and NF-Y transcriptional activator complexes between the promoter and downstream enhancers. Motif rearrangements correlate with differences in tissue-specific expression. Furthermore, homeologs with rearranged motifs could not be transactivated by <i>B. napus</i> CO in transient assays, although they show LD photoperiod-dependent expression. We propose that differential diurnal expression of <i>NF-Y</i> genes contributes to the photoperiod-dependent regulation of <i>B. napus FT</i> genes.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consequences of interspecific plant hybridization on metabolic diversity in naturally occurring hybrid swarms","authors":"Olga Zafra-Delgado,&nbsp;Fabian Schneider,&nbsp;Yoko Nakamura,&nbsp;Michael Reichelt,&nbsp;Jonathan Gershenzon,&nbsp;Frank H. Hellwig,&nbsp;Tobias G. Köllner","doi":"10.1111/tpj.70444","DOIUrl":"https://doi.org/10.1111/tpj.70444","url":null,"abstract":"<p>Interspecific hybridization has influenced plant evolution and diversification. However, how hybridization may affect metabolic diversity, especially in naturally occurring hybridization zones, is unclear. In this study, we selected a <i>Baccharis</i> (Asteraceae) hybrid complex consisting of <i>B. linearis</i>, <i>B</i>. <i>macraei</i>, and <i>B. × intermedia</i> and characterized its metabolic profiles in multiple hybridization zones in central Chile to determine how hybridization affects plant chemistry. Untargeted liquid chromatography–time of flight mass spectrometry analysis of a total of 411 plant individuals collected in the field revealed that the hybrid <i>B. × intermedia</i> combines the metabolic profiles of its two parental species, <i>B. linearis</i> and <i>B</i>. <i>macraei</i>, independent of season, location, and environment. This combinatorial effect was observed in the specialized metabolites, while the primary metabolism did not differ between species. The metabolic diversity of the hybrid exceeded that of the parental species and was influenced by latitude, with higher metabolic diversity in the northern populations than in those in the south. In summary, our results demonstrate that natural interspecific hybridization can quickly increase the diversity of specialized metabolites. This could enhance protection against biotic or abiotic stressors, particularly in changing environmental conditions.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.70444","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Methylation-Activated LaCOMT1 Expression Promotes Cluster Root Formation of White Lupin via a Mechanism Involving the Melatonin Synthesis.","authors":"Qian Zhang, Xing Li, Jiahong Geng-Li, Jinyong Yang, Jingyi Liu, Ke Wang, Yuancan Cheng, Jianping Liu, Feiyun Xu, Zhengrui Wang, Kang Zhang, Jianhua Zhang, Feng Cheng, Weifeng Xu, Wei Yuan","doi":"10.1111/pce.70091","DOIUrl":"https://doi.org/10.1111/pce.70091","url":null,"abstract":"<p><p>White lupin exhibits remarkable adaptability to phosphorus (P)-deficient soil through the development of cluster roots (CR), thereby enhancing P use sufficiency. Despite its crucial role, the underlying mechanism governing CR formation remains elusive. Here, we reveal an elevated DNA methylation level through whole-genome bisulfite sequencing in CR in response to P deficiency, particularly in gene and flanking regions, suggesting a responsive epigenetic mechanism. To further investigate the potential involvement of epigenetic remodelling, we treated lupin plants with the DNA methyltransferase (DNMT) inhibitor 5-azacytidine, which led to a disruption of total DNMT activity and impaired CR formation under phosphorus-deficient conditions. Integrated analysis of methylome and RNA-Seq highlights the methylation of CAFFEIC ACID O-METHYLTRANSFERASE 1 (COMT1), a key enzyme in melatonin synthesis, as pivotal for promoting CR formation in white lupin. Functional validation through overexpression or gene silencing of LaCOMT1 in transgenic lupin roots confirms the positive impact of LaCOMT1 on CR formation. Furthermore, melatonin application directly increases CR numbers, indicating the role of methylation-activated LaCOMT1 in promoting CR formation via melatonin synthesis. Those findings provide insights into the epigenomic landscape of white lupin, establishing a direct genetic link between epigenetic mechanisms and P-deficiency-induced CR formation.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macroscopic appearance and microscopic structure of bark in Indigenous South African species of Meliaceae","authors":"Ekaterina Kotina,&nbsp;Mariam Oyefunke Oyedeji-Amusa,&nbsp;Ben-Erik Van Wyk,&nbsp;Alexei Oskolski","doi":"10.1007/s00468-025-02661-7","DOIUrl":"10.1007/s00468-025-02661-7","url":null,"abstract":"<div><p>Bark structure in seven South African species of <i>Ekebergia</i>, <i>Nymania</i>, <i>Trichilia</i> and <i>Turraea</i> (Meliaceae) was studied to clarify the relationships between its macroscopic appearance and anatomical traits. Like other Meliaceae, these species share the subepidermal initiation of periderm, the presence of phloem fibers, and compound sieve plates. Diagnostic bark characters were revealed to identify these taxa. All studied species share stretching bark showing conspicuous expansion without regular shedding. Their continuity is maintained by anticlinal divisions of phellogen cells and by the formation of expansion cracks, i.e., the superficial disruptions associated with new portions of the periderm. The lenticels found in all studied taxa except <i>Trichilia</i>, unlike expansion cracks, are derived from non-disrupted periderm. The mature bark of <i>E. capensis</i> shows conspicuous radial expansion of secondary phloem by periclinal divisions of axial parenchyma (proliferation tissue), which has not been reported elsewhere. Reticulate fracturing of mature bark in <i>Ekebergia</i> and <i>Trichilia</i> is presumably associated with the presence of elastic parenchymatous layers covered by a rigid periderm. We hypothesize that such elastic layers can redistribute the stressing forces of radial wood increment into the tensile forces stretching the periderm parallel to the bark surface, which cracks not only vertically but also horizontally and diagonally.</p></div>","PeriodicalId":805,"journal":{"name":"Trees","volume":"39 5","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00468-025-02661-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating dominant tree species as bioindicators for urban air pollution mitigation in Ranchi, Eastern India","authors":"Pawan Ekka,&nbsp;Purabi Saikia","doi":"10.1007/s00468-025-02670-6","DOIUrl":"10.1007/s00468-025-02670-6","url":null,"abstract":"<div><h3>Key message</h3><p>Effective nature-based solutions for urban air pollution require understanding how season, site, and species affect tree tolerance. The complex interplay of biochemical parameters significantly impacts trees’ sensitivity and tolerance.</p><h3>Abstract</h3><p>Evaluating dominant tree species as bioindicators can provide valuable insights for effective air pollution mitigation strategies in rapidly developing cities like Ranchi, Eastern India. The air pollution tolerance index (APTI), anticipated performance index (API), and dust capturing capacity (DCC) of dominant urban street trees in Ranchi were studied at control, heavy traffic, and industrial sites during pre- and post-monsoon seasons. APTI varied significantly across seasons (F_1,25.43 = 27.24, p &lt; 0.05), sites (F_2,261.87 = 280.50, p &lt; 0.05), and trees (F_9,9.18 = 9.83, p &lt; 0.05). APTI values were highest at industrial, followed by heavy traffic, and lowest at control sites. AA content was the most influential biochemical parameter associated with APTI, showing a statistically significant strong correlation (r = 0.819, <i>p</i> &lt; 0.01). Street trees at industrial sites exhibited the highest APTI values (range: 15.11–19.99), followed by heavy traffic sites (12.79–18.30), and lowest at control sites (11.46–15.72). DCC also varied significantly across seasons (F_1,0.321 = 17.40, p &lt; 0.05), sites (F_2,2.65 = 144.25, p &lt; 0.05), and trees (F_9,2.95 = 160.92, p &lt; 0.05). The highest DCC was recorded in <i>Melia azedarach</i> (2.64 mg cm⁻²), followed by <i>Bauhinia variegata</i> (2.49 mg cm⁻²), and <i>Bridelia retusa</i> (2.13 mg cm⁻²). APTI, API, and DCC were significantly affected by seasons, sites, species, and their interactions with pollutants. These findings highlight the importance of APTI, API, and DCC as important indicators of the pollution mitigation abilities of different urban street trees to recommend for urban greening initiatives in rapidly urbanizing cities like Ranchi.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":805,"journal":{"name":"Trees","volume":"39 5","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A GDSL lipase confers resistance to piercing-sucking insects in tobacco by strengthening leaf cuticle","authors":"Na Chen,&nbsp;Yu-Die Xiong,&nbsp;Chi Zou,&nbsp;Yu-Wei Zhong,&nbsp;Hui Du,&nbsp;Yu-Jie Chi,&nbsp;Chan Zhao,&nbsp;Shu-Sheng Liu,&nbsp;Xiao-Wei Wang","doi":"10.1111/tpj.70440","DOIUrl":"https://doi.org/10.1111/tpj.70440","url":null,"abstract":"<div>\u0000 \u0000 <p>Piercing-sucking insects, such as whiteflies and aphids, cause massive economic losses in major crops around the world. During feeding, the stylets of piercing-sucking insects navigate cuticles, cell walls, epidermal cells, and mesophyll cells; thus, these barriers are vital for the resistance of plants to insects. However, the relationship between insect stylet probing behavior and the composition and structure of these barriers remains unclear. Here, we identified a tobacco <i>Cuticle Related Factor</i> (<i>NtCRF</i>), which was induced significantly by whitefly infestation. Bioassays showed that <i>NtCRF</i> positively regulated plant resistance against whiteflies and green peach aphids. Silencing of <i>NtCRF</i> did not affect plant jasmonic acid (JA) and salicylic acid (SA) defenses but shortened the stylet probing time of phloem-feeders. Further studies confirmed that silencing of <i>NtCRF</i> resulted in significant structure destruction of the leaf cuticle and led to increased epidermal permeability. Overexpression of <i>NtCRF</i> in Arabidopsis also significantly enhanced the plant's resistance against whiteflies and green peach aphids. Our findings expand understanding of plant–insect interactions and provide a strategy for genetic improvement of crop resistance against piercing-sucking insects.</p>\u0000 </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144897396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic, morphological, and photosynthetic parameters of absorption of nitrogen forms in olive cultivars (Olea europaea L.)","authors":"Ana Luiza Lima Marques,&nbsp;Amanda Veridiana Krug,&nbsp;Gabriel Alberto Sans,&nbsp;Daniéle Gonçalves Papalia,&nbsp;Allan Augusto Kokkonen,&nbsp;Luana Paula Garlet,&nbsp;Betânia Vahl de Paula,&nbsp;Eduardo Maciel Haitzmann dos Santos,&nbsp;Luciane Almeri Tabaldi,&nbsp;William Natale,&nbsp;Vagner Brasil Costa,&nbsp;Gustavo Brunetto","doi":"10.1007/s00468-025-02668-0","DOIUrl":"10.1007/s00468-025-02668-0","url":null,"abstract":"<div><h3>Key message</h3><p>Knowledge of the efficiency of nitrogen absorption contributes to the selection of olive cultivars that are more efficient in absorbing N even at low concentrations in the environment.</p><h3>Abstract</h3><p>Fertilization of olive trees (<i>Olea europaea</i> L.) generally follows standardized nitrogen (N) doses, without considering the specific efficiency of each cultivar in N absorption. The lack of knowledge about kinetic parameters can result in excessive application, increasing environmental risks and impairing oil quality. The study aimed to characterize the most efficient olive cultivars in the absorption of N, NO₃⁻, and NH₄⁺ forms, and to determine whether kinetic, photosynthetic variables, and root morphological parameters contribute to the selection of cultivars that are more efficient in the use of N. Four olive cultivars were grown hydroponically for 21 days in nutrient solution and transferred to 0.03 mol L⁻¹ CaSO₄ solution for 15 days. Subsequently, the plants received nutrient solution again and periodic collection began during a 61-h kinetic absorption march. The cultivar Coratina was the most efficient in NO₃⁻ absorption, presenting higher <i>V</i>_max (maximum absorption speed) and lower <i>K</i>_m (Michaelis–Menten constant) and <i>C</i>_min (minimum concentration) values, which allows N absorption at low concentrations due to the affinity of root transporters. Its greater length, surface area, and quantity of fine roots favored this efficiency. It also presented better photosynthetic parameters and greater N accumulation in roots. Arbequina and Koroneiki had high <i>K</i>_m and <i>C</i>_min for NO₃⁻ and NH₄⁺, while Arbosana presented lower <i>C</i>_min values for NH₄⁺. These results show that different cultivars have distinct nutritional strategies and that physiological and morphological parameters are essential in choosing the best cultivars and optimizing nitrogen fertilization, ensuring greater sustainability and productivity in olive cultivation.</p></div>","PeriodicalId":805,"journal":{"name":"Trees","volume":"39 5","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144897208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenome, repeatome, and transcriptome analysis to comprehend the structural and functional aspects of polyploid Morus serrata Roxb","authors":"Raju Mondal,&nbsp;Himanshu Dubey,&nbsp;A. Ramesha,&nbsp;Kunjupillai Vijayan,&nbsp;V. Nishitha Naik,&nbsp;S. Manthira Moorthy","doi":"10.1007/s00468-025-02665-3","DOIUrl":"10.1007/s00468-025-02665-3","url":null,"abstract":"<div><p>Repeat elements contribute significantly to genome expansions, gene regulation, and expression of functional traits at the increased cost of genome maintenance, especially in polyploids. <i>Morus serrata</i> Roxb. (Moraceae) is a wild tree species having polyploid genome of Himalayan origin. Upon phenotypic analysis, <i>M. serrata</i> exhibited distinct polyploid-associated traits within the <i>Morus</i> species complex, which are impacted by cell size, cell division rate, and stoichiometry balance. Genomic analysis suggests that genome-wide repeated DNA landscape (repeatome), especially DNA transposons played a substantial role in the genome expansions of <i>M. serrata</i>. Furthermore, <i>M. serrata</i> transcriptome yielded overrepresented genes associated with the transposition of transposable elements (TEs) and nucleic acid metabolism. Overall phenome, repeatome, and transcriptome study reveals that <i>M. serrata</i> devotes a significant portion of its transcriptional budget to maintaining a large genome expended by TEs and loses growth superiority compared to studied species of the genus <i>Morus</i> L. The study provided new insights into the structural and functional aspects of natural polyploidization and loss of growth superiority.</p></div>","PeriodicalId":805,"journal":{"name":"Trees","volume":"39 5","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the Role of Actinorhizal Symbioses in Ecosystem Nitrogen Dynamics.","authors":"Bin Hu, Zhenshan Liu, Tong Peng, Man Yin, Rodica Efrose, Emmanouil Flemetakis, Philipp Franken, Heinz Rennenberg","doi":"10.1111/pce.70146","DOIUrl":"https://doi.org/10.1111/pce.70146","url":null,"abstract":"<p><p>Symbiotic associations between plants and microorganisms are crucial to global biogeochemical cycling and ecosystem stability. Mycorrhizal fungi and nitrogen (N₂)-fixing bacteria are recognized as the two main groups of microorganisms involved in such symbiotic interactions. They not only constitute the most wide-spread symbiotic microorganisms, but also ensure plants to acquire additional N resources directly from the atmosphere. Although plant-microbial interactions, for example, the performance of AM-plant and rhizobia-legume plant symbioses, have been well studied and reviewed in detail previously, still less information is known about these processes in actinorhizal symbioses. The present review is aimed to summarize current knowledge of the interaction of partners in actinorhizal root symbioses, in particular the signalling processes during establishment of BNF, and the specificity of and dependency on different symbiotic partners in this interactions, based on evolution and distribution in the plant and microbial kingdom. The features of nutrient transfer in these root symbiotic relationships and the significance of actinorhizal symbioses for the performance of plants under environmental stress are discussed and compared with AM and rhizobia-legume symbioses. In addition, research gaps in actinorhizal root symbioses research are identified and future research avenues are suggested.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"XsLTPG31 Confers Leaf Cuticular Wax Deposition and Drought Resistance in Yellowhorn.","authors":"Huihui Xu, Xiaojuan Liu, Zhuo Ban, Yingying Yang, Lingfeng Zhang, Quanxin Bi, Libing Wang","doi":"10.1111/pce.70144","DOIUrl":"https://doi.org/10.1111/pce.70144","url":null,"abstract":"<p><p>With increasing frequency and severity under climate change, drought stress has become a predominant abiotic limiting factor of global plant productivity. Yellowhorn (Xanthoceras sorbifolium Bunge), an endemic species of woody oil tree in North China, has substantial developmental potential in arid and semi-arid regions. To elucidate the genetic basis of its drought response, a genome-wide association study (GWAS) of 13 leaf anatomical structure traits across 237 yellowhorn accessions was performed in this study, and 21 candidate genes that regulate leaf structural variation were identified. Among these genes, XsLTPG31, which encodes a nonspecific lipid transfer protein, was significantly induced by drought stress. Heterologous overexpression of XsLTPG31 in Arabidopsis, its transient overexpression in yellowhorn leaves, and virus-induced gene silencing (VIGS) of XsLTPG31 demonstrated that XsLTPG31 promotes the deposition of leaf epidermal wax and modulates drought resistance through facilitating the export of wax to the extracellular space. Moreover, we revealed that XsLTPG31 is directly activated by XsMYB16 via promoter binding. Taken together, the results of our study enhance the understanding of the regulatory mechanisms underlying LTPG-mediated cuticular wax deposition and might provide targets for the breeding of drought-tolerant varieties of yellowhorn.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}