XsLTPG31 Confers Leaf Cuticular Wax Deposition and Drought Resistance in Yellowhorn.

IF 6.3 1区 生物学 Q1 PLANT SCIENCES
Huihui Xu, Xiaojuan Liu, Zhuo Ban, Yingying Yang, Lingfeng Zhang, Quanxin Bi, Libing Wang
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Abstract

With increasing frequency and severity under climate change, drought stress has become a predominant abiotic limiting factor of global plant productivity. Yellowhorn (Xanthoceras sorbifolium Bunge), an endemic species of woody oil tree in North China, has substantial developmental potential in arid and semi-arid regions. To elucidate the genetic basis of its drought response, a genome-wide association study (GWAS) of 13 leaf anatomical structure traits across 237 yellowhorn accessions was performed in this study, and 21 candidate genes that regulate leaf structural variation were identified. Among these genes, XsLTPG31, which encodes a nonspecific lipid transfer protein, was significantly induced by drought stress. Heterologous overexpression of XsLTPG31 in Arabidopsis, its transient overexpression in yellowhorn leaves, and virus-induced gene silencing (VIGS) of XsLTPG31 demonstrated that XsLTPG31 promotes the deposition of leaf epidermal wax and modulates drought resistance through facilitating the export of wax to the extracellular space. Moreover, we revealed that XsLTPG31 is directly activated by XsMYB16 via promoter binding. Taken together, the results of our study enhance the understanding of the regulatory mechanisms underlying LTPG-mediated cuticular wax deposition and might provide targets for the breeding of drought-tolerant varieties of yellowhorn.

tpg31对黄角菊叶片表皮蜡沉积和抗旱性的影响。
随着气候变化的频繁和严重,干旱胁迫已成为全球植物生产力的主要非生物限制因素。黄角(Xanthoceras sorbifolium Bunge)是中国北方木本油树的特有种,在干旱半干旱区具有很大的开发潜力。为了阐明黄角干旱响应的遗传基础,本研究对237份黄角植物叶片解剖结构性状进行了全基因组关联分析,鉴定出21个调节叶片结构变异的候选基因。在这些基因中,编码非特异性脂质转移蛋白的xslttpg31受到干旱胁迫的显著诱导。XsLTPG31在拟南芥中的异源过表达、在黄角叶片中的短暂过表达以及病毒诱导的基因沉默(VIGS)表明,XsLTPG31促进了叶片表皮蜡质的沉积,并通过促进蜡质向细胞外空间的输出来调节抗旱性。此外,我们发现XsMYB16通过启动子结合直接激活了XsLTPG31。综上所述,我们的研究结果增强了对ltpg介导的表皮蜡沉积的调控机制的理解,并可能为黄角耐旱品种的选育提供靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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