{"title":"DNA甲基化激活的LaCOMT1表达通过褪黑素合成机制促进白露根茎的形成。","authors":"Qian Zhang, Xing Li, Jiahong Geng-Li, Jinyong Yang, Jingyi Liu, Ke Wang, Yuancan Cheng, Jianping Liu, Feiyun Xu, Zhengrui Wang, Kang Zhang, Jianhua Zhang, Feng Cheng, Weifeng Xu, Wei Yuan","doi":"10.1111/pce.70091","DOIUrl":null,"url":null,"abstract":"<p><p>White lupin exhibits remarkable adaptability to phosphorus (P)-deficient soil through the development of cluster roots (CR), thereby enhancing P use sufficiency. Despite its crucial role, the underlying mechanism governing CR formation remains elusive. Here, we reveal an elevated DNA methylation level through whole-genome bisulfite sequencing in CR in response to P deficiency, particularly in gene and flanking regions, suggesting a responsive epigenetic mechanism. To further investigate the potential involvement of epigenetic remodelling, we treated lupin plants with the DNA methyltransferase (DNMT) inhibitor 5-azacytidine, which led to a disruption of total DNMT activity and impaired CR formation under phosphorus-deficient conditions. Integrated analysis of methylome and RNA-Seq highlights the methylation of CAFFEIC ACID O-METHYLTRANSFERASE 1 (COMT1), a key enzyme in melatonin synthesis, as pivotal for promoting CR formation in white lupin. Functional validation through overexpression or gene silencing of LaCOMT1 in transgenic lupin roots confirms the positive impact of LaCOMT1 on CR formation. Furthermore, melatonin application directly increases CR numbers, indicating the role of methylation-activated LaCOMT1 in promoting CR formation via melatonin synthesis. Those findings provide insights into the epigenomic landscape of white lupin, establishing a direct genetic link between epigenetic mechanisms and P-deficiency-induced CR formation.</p>","PeriodicalId":222,"journal":{"name":"Plant, Cell & Environment","volume":" ","pages":""},"PeriodicalIF":6.3000,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DNA Methylation-Activated LaCOMT1 Expression Promotes Cluster Root Formation of White Lupin via a Mechanism Involving the Melatonin Synthesis.\",\"authors\":\"Qian Zhang, Xing Li, Jiahong Geng-Li, Jinyong Yang, Jingyi Liu, Ke Wang, Yuancan Cheng, Jianping Liu, Feiyun Xu, Zhengrui Wang, Kang Zhang, Jianhua Zhang, Feng Cheng, Weifeng Xu, Wei Yuan\",\"doi\":\"10.1111/pce.70091\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>White lupin exhibits remarkable adaptability to phosphorus (P)-deficient soil through the development of cluster roots (CR), thereby enhancing P use sufficiency. Despite its crucial role, the underlying mechanism governing CR formation remains elusive. Here, we reveal an elevated DNA methylation level through whole-genome bisulfite sequencing in CR in response to P deficiency, particularly in gene and flanking regions, suggesting a responsive epigenetic mechanism. To further investigate the potential involvement of epigenetic remodelling, we treated lupin plants with the DNA methyltransferase (DNMT) inhibitor 5-azacytidine, which led to a disruption of total DNMT activity and impaired CR formation under phosphorus-deficient conditions. Integrated analysis of methylome and RNA-Seq highlights the methylation of CAFFEIC ACID O-METHYLTRANSFERASE 1 (COMT1), a key enzyme in melatonin synthesis, as pivotal for promoting CR formation in white lupin. Functional validation through overexpression or gene silencing of LaCOMT1 in transgenic lupin roots confirms the positive impact of LaCOMT1 on CR formation. Furthermore, melatonin application directly increases CR numbers, indicating the role of methylation-activated LaCOMT1 in promoting CR formation via melatonin synthesis. Those findings provide insights into the epigenomic landscape of white lupin, establishing a direct genetic link between epigenetic mechanisms and P-deficiency-induced CR formation.</p>\",\"PeriodicalId\":222,\"journal\":{\"name\":\"Plant, Cell & Environment\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":6.3000,\"publicationDate\":\"2025-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant, Cell & Environment\",\"FirstCategoryId\":\"2\",\"ListUrlMain\":\"https://doi.org/10.1111/pce.70091\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant, Cell & Environment","FirstCategoryId":"2","ListUrlMain":"https://doi.org/10.1111/pce.70091","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
DNA Methylation-Activated LaCOMT1 Expression Promotes Cluster Root Formation of White Lupin via a Mechanism Involving the Melatonin Synthesis.
White lupin exhibits remarkable adaptability to phosphorus (P)-deficient soil through the development of cluster roots (CR), thereby enhancing P use sufficiency. Despite its crucial role, the underlying mechanism governing CR formation remains elusive. Here, we reveal an elevated DNA methylation level through whole-genome bisulfite sequencing in CR in response to P deficiency, particularly in gene and flanking regions, suggesting a responsive epigenetic mechanism. To further investigate the potential involvement of epigenetic remodelling, we treated lupin plants with the DNA methyltransferase (DNMT) inhibitor 5-azacytidine, which led to a disruption of total DNMT activity and impaired CR formation under phosphorus-deficient conditions. Integrated analysis of methylome and RNA-Seq highlights the methylation of CAFFEIC ACID O-METHYLTRANSFERASE 1 (COMT1), a key enzyme in melatonin synthesis, as pivotal for promoting CR formation in white lupin. Functional validation through overexpression or gene silencing of LaCOMT1 in transgenic lupin roots confirms the positive impact of LaCOMT1 on CR formation. Furthermore, melatonin application directly increases CR numbers, indicating the role of methylation-activated LaCOMT1 in promoting CR formation via melatonin synthesis. Those findings provide insights into the epigenomic landscape of white lupin, establishing a direct genetic link between epigenetic mechanisms and P-deficiency-induced CR formation.
期刊介绍:
Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.