European Biophysics Journal最新文献

Mechanical properties of adherent cell sheets analyzed by deflection of supporting membrane. 用支撑膜挠曲分析贴壁细胞片的力学性能。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2025-09-29 DOI: 10.1007/s00249-025-01798-0

Yuri M Efremov, Anastasia M Subbot, Ivan A Novikov, Sergei E Avetisov, Peter S Timashev

{"title":"Mechanical properties of adherent cell sheets analyzed by deflection of supporting membrane.","authors":"Yuri M Efremov, Anastasia M Subbot, Ivan A Novikov, Sergei E Avetisov, Peter S Timashev","doi":"10.1007/s00249-025-01798-0","DOIUrl":"https://doi.org/10.1007/s00249-025-01798-0","url":null,"abstract":"Multicellular structures, including cell sheets, are actively used as model systems to study intercellular interactions and can be applied in different areas of regenerative medicine. In this paper, we present a novel approach for measuring mechanical properties of cell sheets based on a simple experimental setup and numerical simulations. The advantage of the present approach is the relative ease of the sample preparation, while previous systems for the tensile tests required specialized and sensitive equipment. With the developed approach, the cell sheet on a polymer membrane is mounted in the holder on one side, and then deflection of the free end of the membrane is measured. The deflection of this cantilever-like construction depends on the elastic modulus of the membrane (which is known) and cell sheet, and also from the traction force generated by the cell sheet. By involving an experimental step with relaxing the traction force and by conducting finite element simulations, both traction force and elastic properties of the cell sheet can be estimated. We performed such measurements on cell sheets from keratocytes from corneal explants and confirmed that the developed approach is applicable for measurement of both traction force and elastic modulus of cell sheets.","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysics in the Czech Republic: 70th anniversary of the institute of biophysics. 捷克共和国的生物物理学：生物物理研究所成立70周年。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2025-09-28 DOI: 10.1007/s00249-025-01795-3

Eva Bártová, Stanislav Kozubek, Aleš Kovařík

引用次数: 0

Proceedings of the 26th International Analytical Ultracentrifugation Workshop and Symposium. 第26届国际分析超离心研讨会论文集。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2025-09-27 DOI: 10.1007/s00249-025-01799-z

Johannes Walter, Alexander Bepperling

{"title":"Proceedings of the 26th International Analytical Ultracentrifugation Workshop and Symposium.","authors":"Johannes Walter, Alexander Bepperling","doi":"10.1007/s00249-025-01799-z","DOIUrl":"https://doi.org/10.1007/s00249-025-01799-z","url":null,"abstract":"The 26th International Analytical Ultracentrifugation Workshop and Symposium (AUC2024) took place at the scenic Banz Abbey near Bad Staffelstein, Germany, from July 22 to 27, 2024. 84 participants from 16 countries (Belgium 1, Canada 7, China 3, Colombia 1, Czech Republic 3, Finland 1, France 3, Germany 35, Israel 1, Italy 1, Japan 2, New Zealand 2, Spain 1, Switzerland 4, United Kingdom 5, United States 14) travelled to Germany to present and discuss the latest advances in the field. 40 workshop sessions were held by world-leading experts covering all aspects of AUC including experimental design, data analysis and data processing according to good manufacturing practice (GMP), but also complementary methods such as hydrodynamic modelling, isothermal titration calorimetry and small angle X-ray scattering data processing were considered. A visit to a stalactite cave in Franconian Switzerland and the Bavarian beer museum in Kulmbach offered a welcome change to the scientific program and started off the 3-day symposium. The presentations featured of course AUC, but also dynamic and static light scattering, small angle X-ray and neutron scattering, surface plasmon resonance, field flow fractionation, calorimetry, chromatography, and electron microscopy. The AUC2024 special volume provides a comprehensive overview of the sustained innovation, utility and relevance of AUC and related solution biophysical and particle technology methods across various disciplines, including biochemistry, structural biology, synthetic polymer chemistry, carbohydrate chemistry, protein and nucleic acid characterization, nano and colloids science, and macromolecular interactions.","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145172147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial alterations induced by Macrovipera lebetina venom. 大毒蛇毒液诱导线粒体改变。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2025-09-14 DOI: 10.1007/s00249-025-01796-2

Virjinia Doltchinkova, Victoria Vitkova, Milena Shkodrova, Meglena Kitanova, Vessela Lozanova, Siya Lozanova, Avgust Ivanov, Chavdar Roumenin

{"title":"Mitochondrial alterations induced by Macrovipera lebetina venom.","authors":"Virjinia Doltchinkova, Victoria Vitkova, Milena Shkodrova, Meglena Kitanova, Vessela Lozanova, Siya Lozanova, Avgust Ivanov, Chavdar Roumenin","doi":"10.1007/s00249-025-01796-2","DOIUrl":"https://doi.org/10.1007/s00249-025-01796-2","url":null,"abstract":"The development of snake venom-based therapeutics and antivenoms is closely linked to understanding how snake venoms modulate the physicochemical properties of biological membranes. In this study, we investigated the effects of Macrovipera lebetina venom on the surface electrical characteristics of rat liver mitochondrial membranes in vitro. A combination of electrokinetic and mechanical techniques was employed, including measurements of mitochondrial electrophoretic mobility and the bending elasticity of phosphatidylcholine membrane models. Exposure to Macrovipera lebetina venom significantly increased the net surface charge of mitochondria, while adenosine triphosphatase (ATPase) activity remained unchanged. Morphological alterations and vesicle aggregation, observed via light scattering and fluorescence microscopy, indicated reduced binding of α-D-mannosyl and α-D-glucosyl residues to the outer mitochondrial membrane in the presence of venom. In addition, malondialdehyde assays revealed elevated levels of oxidized lipid species in venom-treated mitochondria. A pronounced softening of model phosphatidylcholine membranes was also observed, likely associated with lipid oxidation induced by the venom. It states that the effect of the venom of the Macrovipera lebetina on the surface electrical charge of rat liver mitochondria is being studied in vitro. The surface charge is a physicochemical characteristic of the mitochondrial membrane.","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Voltage-sensing domains: structural and functional diversity. 电压传感领域：结构和功能的多样性。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2025-09-13 DOI: 10.1007/s00249-025-01797-1

Martin C Heiss, Bernhard E Flucher

引用次数: 0

Ribulose-1,5-bisphosphate carboxylase/oxygenase activase isoforms from diverse species show differences in oligomeric structure, thermal stability, and activity. 不同物种的核酮糖-1,5-二磷酸羧化酶/加氧酶激活酶异构体在寡聚物结构、热稳定性和活性方面存在差异。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2025-09-11 DOI: 10.1007/s00249-025-01794-4

Jeremy R Keown, Serena A J Watkin, F Grant Pearce

{"title":"Ribulose-1,5-bisphosphate carboxylase/oxygenase activase isoforms from diverse species show differences in oligomeric structure, thermal stability, and activity.","authors":"Jeremy R Keown, Serena A J Watkin, F Grant Pearce","doi":"10.1007/s00249-025-01794-4","DOIUrl":"https://doi.org/10.1007/s00249-025-01794-4","url":null,"abstract":"Rubisco activase (Rca) is critical for maintaining Rubisco activity during photosynthesis by removing inhibitory sugar phosphates through ATP hydrolysis. Despite its importance, the structural and functional diversity of Rca across species remain poorly understood. This study compares the oligomeric assembly, thermal stability, and functional activities of α- and β-isoforms of Rca from cotton, creosote, Antarctic hairgrass, and Sitka spruce, representing diverse thermal and ecological adaptations. We found that cotton and creosote Rca isoforms form highly polydisperse complexes in solution, with no evidence of discrete hexamer formation, even in the presence of Mg.ATPγS. In contrast, Antarctic hairgrass α-Rca and Sitka spruce β-Rca formed stable hexamers under similar conditions. Spruce α-Rca exhibited unique redox-dependent oligomerization, forming large complexes stabilized by disulfide bonds. Thermal stability assays revealed significant nucleotide-induced stabilization in most isoforms, with hexamer formation enhancing stability and activity in select cases. Functional assays showed that hexamer-forming isoforms displayed superior Rubisco reactivation and ATP hydrolysis activities, even at low protein concentrations, while smaller oligomeric assemblies also supported activity in some species. These findings provide new insights into the structural and functional adaptations of Rca, highlighting the role of oligomeric assembly and environmental influences on its activity. This work lays a foundation for improving photosynthetic efficiency by targeting Rca isoforms tailored to specific environmental conditions.","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical characterization of polyphenol aggregates in Moringa oleifera leaves water extract: stability and surface exposure effect on antioxidant activity under dilution. 辣木叶水提物中多酚聚集体的生物物理特性：稀释条件下稳定性及表面暴露对抗氧化活性的影响。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2025-09-03 DOI: 10.1007/s00249-025-01786-4

Rita Carrotta, Fabio Librizzi, Vincenzo Martorana, Samuele Raccosta, Maria Rosalia Mangione

{"title":"Biophysical characterization of polyphenol aggregates in Moringa oleifera leaves water extract: stability and surface exposure effect on antioxidant activity under dilution.","authors":"Rita Carrotta, Fabio Librizzi, Vincenzo Martorana, Samuele Raccosta, Maria Rosalia Mangione","doi":"10.1007/s00249-025-01786-4","DOIUrl":"https://doi.org/10.1007/s00249-025-01786-4","url":null,"abstract":"The aqueous extract of Moringa oleifera leaves has been previously characterized for its polyphenolic composition, yet the behavior of its colloidal aggregates under dilution remains largely unexplored. In this study, we investigate the structural and chemical properties of these aggregates at room temperature, focusing on their stability and surface exposure upon dilution. Although the aggregates break up as dilution increases, they never fully dissolve within the conditions explored. Both multi-angle static light scattering and dynamic light scattering highlight aggregates fragmentation and size heterogeneity under dilution. UV-vis absorption spectroscopic data strongly suggest that the aggregates of different sizes present in the extract are homogeneously constituted, as their spectra are similar to those of the main polyphenol components. The Folin-Ciocâlteu assay reveals an increase in gallic acid equivalent values normalized for extract concentration, suggesting that fragmentation prompted by dilution enhances the exposure of reactive sites. A very basic model, considering only one kind of aggregate with uniform density, is employed to support this interpretation. Assuming this model, the Folin-Ciocâlteu assay data allow to grasp the law regulating the change of the aggregate average size under dilution, i.e., a power law. Additionally, in-liquid atomic force microscopy imaging confirms the presence of smaller but still aggregated particles at high dilution, enabling the calculation of a height distribution, that is consistent with the model prediction. These findings provide insights into the dynamic behavior of polyphenol-rich aggregates in aqueous systems and their potential implications for bioavailability and reactivity.","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrodynamic properties of macromolecules and nanoparticles in dilute solution: a brief essay on classical and modern concepts. 大分子和纳米颗粒在稀溶液中的流体动力学性质：古典和现代概念的简要介绍。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2025-08-24 DOI: 10.1007/s00249-025-01791-7

José García de la Torre, José G Hernández-Cifre

引用次数: 0

Optimizing electroporation via pulse modulation: a molecular dynamics study. 通过脉冲调制优化电穿孔：分子动力学研究。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2025-08-21 DOI: 10.1007/s00249-025-01793-5

Shahariar Emon, Al Amin, Md Hossain, Shovon Saha, Md Asaduzzaman, Md Lokman Hossen, Mohammad Abu Sayem Karal, Hiromitsu Takaba, Md Khorshed Alam

{"title":"Optimizing electroporation via pulse modulation: a molecular dynamics study.","authors":"Shahariar Emon, Al Amin, Md Hossain, Shovon Saha, Md Asaduzzaman, Md Lokman Hossen, Mohammad Abu Sayem Karal, Hiromitsu Takaba, Md Khorshed Alam","doi":"10.1007/s00249-025-01793-5","DOIUrl":"https://doi.org/10.1007/s00249-025-01793-5","url":null,"abstract":"Efficient molecular transport via reversible electroporation requires sustained existence of the pore without causing irreversible cellular damage. In this study, we used molecular dynamics simulations to investigate pore formation during electroporation, and we characterized the transition to hydrophilic pores. Our simulations reveal that during the hydrophilic state, the reapplication of an electric field, even at reduced magnitudes, extends the pore duration while maintaining structural integrity. Furthermore, we established that the pore size can be controlled by regulating the intervals between successive electric field pulses, offering precise control over membrane permeabilization. These findings provide a foundation for fine-tuning electroporation protocols, enabling customized permeabilization strategies based on the properties of the molecules to be delivered. This approach has the potential to significantly improve the efficacy of targeted drug delivery and gene therapy. It also creates new possibilities for precise and controlled cellular manipulation in therapeutic contexts.","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical label-free detection of SARS-CoV-2: investigating platform spectroscopic properties for oligonucleotide targeting. 光学无标记检测SARS-CoV-2：寡核苷酸靶向平台光谱特性研究

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2025-08-11 DOI: 10.1007/s00249-025-01787-3

Silvia Maria Cristina Rotondi, Paolo Canepa, Silvia Dante, Maurizio Canepa, Ornella Cavalleri

{"title":"Optical label-free detection of SARS-CoV-2: investigating platform spectroscopic properties for oligonucleotide targeting.","authors":"Silvia Maria Cristina Rotondi, Paolo Canepa, Silvia Dante, Maurizio Canepa, Ornella Cavalleri","doi":"10.1007/s00249-025-01787-3","DOIUrl":"https://doi.org/10.1007/s00249-025-01787-3","url":null,"abstract":"We characterized a DNA/gold interface designed for the detection of the SARS-CoV-2 RNA-dependent RNA polymerase/Helicase (RdRp/Hel) sequence. Using broadband spectroscopic ellipsometry (SE) and a difference spectra approach, we monitored molecular modifications at the interface, from probe sequence deposition to the insertion of a molecular spacer and subsequent hybridization with the target. The UV region revealed the characteristic DNA absorption peak around 260 nm, while changes in δΔ in the NIR correlated with increased optical thickness following each deposition step. The optical response was analyzed as a function of target concentration, and the binding affinity curve, derived from δΔ values at 800 nm, was fitted using a first-order Langmuir model, yielding a dissociation constant KD = (70 ± 10) nM, consistent with literature values. Selectivity studies demonstrated that the interface effectively discriminates the SARS-CoV-2 sequence from the SARS-CoV HKU variant, even in a crowded environment. A complementary platform targeting the SARS-CoV HKU sequence confirmed selective detection of HKU over SARS-CoV-2. These findings highlight the potential for parallel detection of different viral sequences.","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0