European Biophysics Journal最新文献

Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers. 用光学镊子研究病毒自组装、病毒基因组包装和病毒与细胞相互作用的动力学。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-04-30 DOI: 10.1007/s00249-026-01843-6

Yanping Gong, Dorus T Harmsen, Wouter H Roos

{"title":"Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers.","authors":"Yanping Gong, Dorus T Harmsen, Wouter H Roos","doi":"10.1007/s00249-026-01843-6","DOIUrl":"https://doi.org/10.1007/s00249-026-01843-6","url":null,"abstract":"<p><p>Viruses are complex supramolecular assemblies that propagate their genetic material from cell to cell, thereby relying on host cell mechanisms. Employing a combination of passive and active strategies, they efficiently package, transport and release nucleic acids. While structural and biochemical techniques offer insights into certain, static aspects of the viral life cycle, recent advancements in biophysical approaches now allow for direct measurement of their inherent dynamic activities in the research field commonly referred to as physical virology. One of these methods is optical tweezers, enabling the precise measurement of force and position at the single-molecule level over time. Over the past decades, the ability to optically trap beads and to manipulate biomolecules has revolutionised medical and biophysical research. In this paper, we provide a comprehensive analysis of optical tweezers, exploring its integration with imaging modalities and review its diverse applications in the study of viruses and viral components. In particular we focus on studies that use optical tweezers to study virus-cell interactions, genome packaging using molecular motors and co-assembly of viral assembly proteins with their nucleic acid.</p>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Process-analytical study of IL-6 refolding during batch buffer exchange. 间歇缓冲液交换过程中IL-6再折叠的过程分析研究。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-04-22 DOI: 10.1007/s00249-026-01845-4

Yuichi Nakahara, Yuta Endo, Yutaka Matsuda, Shunsuke Yamazaki, Mayumi Watanabe, Yutaka Sato, Masayo Date, Naoyuki Yamada, Yoshiyuki Takahara, Kazutoshi Takahashi, Keisuke Kato, Takahiro Okasora, Daisuke Ejima

引用次数: 0

Statistical mechanics of soft collagenous tissues: a constitutive model with damage for cerebral bridging veins. 软胶原组织的统计力学：脑桥静脉损伤本构模型。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-04-16 DOI: 10.1007/s00249-026-01839-2

Silvia García-Vilana, David Sánchez-Molina, Hamed Abdi, Vafa Rahimi-Movaghar

引用次数: 0

Flow-induced dispersion analysis for probing cyclodextrin-guest binding interactions. 流动诱导分散分析探测环糊精-客体结合相互作用。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-04-15 DOI: 10.1007/s00249-026-01842-7

Josef Hamacek, Federico Perche

引用次数: 0

Advancing Italian biophysics: insights from the 27th SIBPA congress 推进意大利生物物理学：来自第27届SIBPA大会的见解。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-04-11 DOI: 10.1007/s00249-026-01840-9

Silvia Caponi, Rita Carrotta, Paolo Bianchini, Velia Minicozzi, Alberto Diaspro

引用次数: 0

Indenting multicellular spheroids with various tip geometries 具有各种尖端几何形状的缩进多细胞球体。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-04-06 DOI: 10.1007/s00249-026-01838-3

Kajangi Gnanachandran, Ewelina Lorenc, Alessandro Podestà, Małgorzata Lekka

{"title":"Indenting multicellular spheroids with various tip geometries","authors":"Kajangi Gnanachandran, Ewelina Lorenc, Alessandro Podestà, Małgorzata Lekka","doi":"10.1007/s00249-026-01838-3","DOIUrl":"10.1007/s00249-026-01838-3","url":null,"abstract":"<div>\u0000 \u0000 <p>Spheroids are of great interest in the study of cancer as they can partially mimic the tumour microenvironment, thus allowing to investigate several aspects of cell – microenvironment interactions in healthy and diseased conditions, including those pertaining to mechanobiology. Atomic Force Microscopy (AFM) is a versatile tool for studying biological samples and their mechanobiological properties. In AFM, the tip shape and dimensions determine the contact geometry between the tip and the sample and the length scales at which the mechanical properties are probed. Given the complex multiscale structure of spheroids, the choice of tip geometry and size would allow, in principle, to dissect the mechanical response of the overall system into the contributions of the constituents, from the single cell level to the cellular aggregate. In this work, we studied the mechanical properties of spheroids derived from four cell lines (A549, NHLF, HT-29, and CCD-18Co cells). Our studies revealed that using different contact geometries in the fitting procedure results in significantly different Young’s modulus values, highlighting the multiscale response of these complex cellular systems and the importance of a precise experimental design and choice of the AFM probe for the nanomechanical measurements. We observed that the location of F-actin filaments is correlated with the rigidity of the spheroids.</p>\u0000 </div>","PeriodicalId":548,"journal":{"name":"European Biophysics Journal","volume":"55 :","pages":"319 - 330"},"PeriodicalIF":2.4,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00249-026-01838-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measuring bacterial oxygen consumption rate to probe metabolic signature and antimicrobial susceptibility. 通过测量细菌耗氧量来探测代谢特征和抗菌药物敏感性。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-03-28 DOI: 10.1007/s00249-026-01834-7

Chiara Scribani Rossi, Simone Angeli, Bruno Casciaro, Maria Rosa Loffredo, Maria Luisa Mangoni, Sharon Spizzichino, Giovanna Boumis, Manuel Espinosa-Urgel, Marzia Arese, Alessio Paone, Francesca Cutruzzolà, Serena Rinaldo

引用次数: 0

Antisecretory Factor in rat cerebellar granule cells augments inhibitory current via GABA_A receptors: a mechanism for vertigo reduction in Menière's disease? 大鼠小脑颗粒细胞中的抗分泌因子通过GABAA受体增强抑制电流：meni<s:1>病眩晕减轻的机制？

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-03-24 DOI: 10.1007/s00249-026-01837-4

Aroldo Cupello, Virginia Bazzurro, Alberto Diaspro, Elena Gatta, Kliment Gatzinsky, Eva Jennische, Stefan Lange, Mauro Robello, Elena Angeli

引用次数: 0

Analysing the structure of antibodies using circular dichroism with an antibody augmented reference set and the algorithm SELCON. 基于抗体扩增参考集和SELCON算法的圆二色性抗体结构分析。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-03-24 DOI: 10.1007/s00249-026-01828-5

Søren Vrønning Hoffmann, Maria G Bruque, Nykola C Jones, Alison Rodger, Jean Aucamp, Tim R Dafforn, Owen R T Thomas

引用次数: 0

Quantitative analysis of mucociliary activity by computational high-speed video reflection microscopy. 计算高速视频反射显微镜定量分析纤毛黏液活性。

IF 2.4 4区生物学

European Biophysics Journal Pub Date : 2026-03-16 DOI: 10.1007/s00249-026-01835-6

Martin Schneiter, Julian Wyss, Jeremy Schori, Patrik Arnold, Martin Frenz, Pietro Cicuta, Stefan A Tschanz, André Stefanov, Loretta Müller

引用次数: 0