Journal of Photochemistry and Photobiology最新文献

{"title":"A low acriflavine dose strongly potentiates the antimicrobial effect of blue light on Staphylococcus aureus","authors":"Rachel Allen ,&nbsp;Yinka Somorin ,&nbsp;Matthew Slemon ,&nbsp;Eva Zekaite ,&nbsp;Conall Haugh ,&nbsp;Chloe Hobbs ,&nbsp;Merve S. Zeden ,&nbsp;James P. O’Gara ,&nbsp;Conor O’Byrne","doi":"10.1016/j.jpap.2025.100264","DOIUrl":"10.1016/j.jpap.2025.100264","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> infections are difficult to treat in chronic wounds due to biofilm formation and are frequently compounded by antibiotic resistance, necessitating the development of alternative therapeutic approaches. This study investigated the antimicrobial effect of 470 nm blue light, alone and in combination with the photosensitizer, acriflavine, against <em>S. aureus</em>. Planktonic cells and preformed biofilms of <em>S. aureus</em> SH1000 (methicillin-sensitive) and BH1CC (methicillin-resistant) strains were exposed to 470 nm blue light at varying intensities. A reduction of 4.9 and 5.3 log₁₀ was observed in the viability of BH1CC and SH1000 planktonic cells respectively when exposed to blue light at 28 mW cm^-2 for 4 h compared to unexposed cells. The effectiveness of blue light inactivation was reduced at 14 and 7 mW cm^-2, and no inactivation was observed at 3.5 mW cm^-2. Exposure to a combination of 5 μM acriflavine and blue light (3.5 mW cm^-2) significantly reduced BH1CC viability by 6 log₁₀ (<em>p</em> = 0.0079) when compared to blue light alone after 1 h. No SH1000 cells survived 1 h exposure to 3.5 and 1.75 mW cm^-2 combined with 5 μM acriflavine. Incubation of <em>S. aureus</em> strains with any of the tested concentrations of acriflavine in the dark produced no loss of viability, confirming the synergistic action of blue light combined with acriflavine. These results demonstrate that 470 nm blue light is lethal to <em>S. aureus</em> even at very low intensities and that this antimicrobial activity can be significantly enhanced by acriflavine at much lower concentrations than previously reported. These data also suggest that the antimicrobial mode of action for acriflavine is likely to be at least partly light mediated, a finding that has not previously been recognised.</div></div>","PeriodicalId":375,"journal":{"name":"Journal of Photochemistry and Photobiology","volume":"28 ","pages":"Article 100264"},"PeriodicalIF":3.261,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate change: temperature and oxygen impacts on the photooxidation of dissolved organic carbon (DOC)","authors":"Ora E. Johannsson ,&nbsp;Marcio S. Ferreira ,&nbsp;Anne Crémazy ,&nbsp;Gudrun De Boeck ,&nbsp;Adalberto L. Val ,&nbsp;Chris M. Wood","doi":"10.1016/j.jpap.2025.100262","DOIUrl":"10.1016/j.jpap.2025.100262","url":null,"abstract":"<div><div>Over the past 20 years, the Amazon has experienced extreme floods, droughts and warmer temperatures due to climate change. Water temperature in the Rio Negro, a major tributary of the Amazon River, reached it highest October value during the 2023 drought. In Lake Tefé, connected to the Rio Solimões (another major tributary of the Amazon river), water temperatures reached 39°C. Increasing temperatures, and associated decreases in oxygen, will plague the Amazon and other regions, altering and accelerating links in the carbon cycle, such as photooxidation of dissolved organic carbon (DOC). We determined the response of DOC photooxidation rate in the Rio Negro (black-water) and Rio Solimões (white-water) to increases in water temperature between 20°C and 40°C and oxygen concentration between 0.01 mg O₂.l^-1 and 8 mg O₂.l^-1. The temperature coefficient (Q10) averaged 1.165, indicative of the dominance of diffusive processes, presumably of reactive oxygen species involved in photooxidation. Direct kinetic release of CO₂ was 15% to 21% of normoxic CO₂ production, and did not respond to temperature. The activation energy (Ea) of photooxidation was 13.14 kJ.mol^-1 in the Rio Solimões and14.09 kJ.mol^-1 in the Rio Negro. The Eas were not significantly different, suggesting no differences in the cost of photooxidation between the two rivers. They align with UVB Eas. Photooxidative production of CO₂ only became oxygen limited between 0.5 mgO₂.l^-1 – 0.8 mgO₂.l^-1 (1.2 kPa – 1.9 kPa, 23°C). Thus, near-surface levels of oxygen are unlikely to directly depress CO₂ production of DOC as temperatures rise.</div></div>","PeriodicalId":375,"journal":{"name":"Journal of Photochemistry and Photobiology","volume":"28 ","pages":"Article 100262"},"PeriodicalIF":3.261,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A microarray assessment of the modulation of human oxidative stress gene expression by rooibos and honeybush tea extracts in UVB-irradiated keratinocytes","authors":"Lana Keet ,&nbsp;Nashia Deepnarain ,&nbsp;Danicke Willemse ,&nbsp;Stefan Abel ,&nbsp;Ann Louw ,&nbsp;Mariska Lilly","doi":"10.1016/j.jpap.2025.100263","DOIUrl":"10.1016/j.jpap.2025.100263","url":null,"abstract":"<div><div>Ultraviolet B (UVB) radiation can lead to sunburn, premature skin aging, and skin cancer by promoting the formation of harmful reactive oxygen species (ROS) in skin cells. This study investigated the effects of rooibos and honeybush tea extracts on the expression of oxidative stress and antioxidant response genes in keratinocytes exposed to UVB. Microarray was used to investigate the expression of 84 oxidative stress response genes in response to rooibos and honeybush tea extracts and the effect of pre-exposure to extracts prior to UVB exposure was assessed. The results showed that both rooibos and honeybush tea extracts had antioxidant effects by enhancing the expression of various genes involved in the cellular defence against the damaging effects of UVB. Rooibos and honeybush extracts increased the expression of superoxide dismutases (SODs) and glutathione-related genes in skin cells associated with antioxidant enzymes. The increased expression of these genes indicates the antioxidant properties of the extracts. The tea extracts, especially honeybush, upregulated the expression of glutathione synthesis-related genes (GCLM and GCLC), which play a crucial role in maintaining cellular redox balance. Both tea extracts enhanced the expression of various genes involved in the reduction of hydrogen peroxide and lipid peroxides to prevent cellular damage, such as glutathione peroxidases (GPXs) and peroxiredoxins (PRDXs). The expression of several genes was dependent on the presence of UVB and the time after exposure. Overall, the results suggest that rooibos and honeybush tea extracts have antioxidant properties and can influence various cellular pathways involved in responding to oxidative stress and UVB radiation in skin cells. These findings support the potential benefits of these tea extracts in protecting the skin from UV-induced damage.</div></div>","PeriodicalId":375,"journal":{"name":"Journal of Photochemistry and Photobiology","volume":"26 ","pages":"Article 100263"},"PeriodicalIF":3.261,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different light/dark condition modulation of the daily rhythm of insulin and glucagon in dogs","authors":"Claudia Giannetto ,&nbsp;Marilena Briglia ,&nbsp;Francesca Arfuso ,&nbsp;Enrico Cancellieri ,&nbsp;Elisabetta Giudice ,&nbsp;Giuseppe Piccione ,&nbsp;Maria Rizzo","doi":"10.1016/j.jpap.2025.100261","DOIUrl":"10.1016/j.jpap.2025.100261","url":null,"abstract":"<div><div>Considering the importance of glucose homeostasis and knowing that the alteration of its regulation is the cause of many dogs’ pathologies, the present study aimed to investigate insulin, glucagon, and glucose daily rhythmicity in dogs subjected to a light/dark cycle (15/9 - L/D) and constant light (L/L). Five Beagle dogs (2 years old, male, 14.00 ± 0.50 kg) were housed in individual boxes, fed at 17:00, with water <em>ad libitum</em>; blood samples were collected every 4 h over 24 h for each photoperiod. A daily rhythmicity of insulin and glucagon was observed. Insulin acrophase was observed in the dark phase of the L/D cycle; glucagon acrophase was observed at the beginning of the light phase of the L/D cycle, for both parameters it was anticipated for about 4 h in the L/L cycle. The glucose oscillation trend remained constant during both photoperiods. Insulin and glucagon were positively correlated both in L/D and L/L cycles, the temporal difference of their oscillation of about 4 h persisted in both photoperiods. These findings contribute to the field of dogs’ circadian physiology and should be taken into consideration during the dogs’ clinical evaluation, particularly for the diagnosis and treatment of blood glucose homeostasis alterations.</div></div>","PeriodicalId":375,"journal":{"name":"Journal of Photochemistry and Photobiology","volume":"26 ","pages":"Article 100261"},"PeriodicalIF":3.261,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Er:YAG laser bone bed milling, with or without photobiomodulation, on the bone repair process of additive manufacturing implants in rats","authors":"Fernando Costa Neto ,&nbsp;Isadora Breseghello ,&nbsp;Laura Vidoto Paludetto ,&nbsp;Sabrina Cruz Tfaile Frasnelli ,&nbsp;Fábio Roberto de Souza Batista ,&nbsp;Alberto Blay ,&nbsp;Armando Boni ,&nbsp;Fernando Costa Júnior ,&nbsp;Roberta Okamoto","doi":"10.1016/j.jpap.2025.100260","DOIUrl":"10.1016/j.jpap.2025.100260","url":null,"abstract":"<div><div>The preparation of the bone bed for implant placement is a critical factor for successful osseointegration and is influenced by surgical techniques and instrumentation. In this context, a study was conducted to compare bone bed milling using an Er:YAG laser, with or without photobiomodulation, to conventional milling combined with Nd:YAG laser photobiomodulation. To assess the effects of Er:YAG laser milling, with or without photobiomodulation, on bone bed preparation and its potential to enhance osseointegration, 28 male rats underwent implant placement in their tibial metaphyses and were divided into four groups: conventional milling (CM), photobiomodulation + conventional milling (PBM + CM), Er:YAG laser milling (Er:YAG), and photobiomodulation + Er:YAG laser milling (PBM + Er:YAG). Euthanasia was performed 28 days after implant placement. Reverse torque testing and real-time PCR (RT-PCR) analysis were conducted to evaluate the expression of osteogenic markers, including ALP, IBSP, OCN, and RUNX2. Data were analyzed using the Shapiro-Wilk test, followed by one-way ANOVA and Tukey's post hoc test, where applicable, with a significance level of <em>p</em> ≤ 0.05. The Er:YAG group exhibited a significantly higher implant removal torque compared to the CM group. Additionally, the expression of genes associated with bone maturation (OCN), differentiation (RUNX2), and osteoblastic activity (ALP and IBSP) was significantly increased in both the Er:YAG and PBM + Er:YAG groups, indicating enhanced osteogenic potential. Thus, Er:YAG laser milling, with or without photobiomodulation using the Nd:YAG laser, may represent a promising alternative for bone bed preparation in implant procedures. However, further studies are required to better characterize bone healing and its long-term consolidation.</div></div>","PeriodicalId":375,"journal":{"name":"Journal of Photochemistry and Photobiology","volume":"26 ","pages":"Article 100260"},"PeriodicalIF":3.261,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonthermal atmospheric pressure plasma (NTAPP) effectively eliminates skin tumors in a mouse model of UV-induced carcinogenesis with a short-term treatment regimen","authors":"Szabolcs Bozsányi ,&nbsp;Ruby Acquah ,&nbsp;Rhea Carmel Glen Rodrigues ,&nbsp;Erin C. Tracy ,&nbsp;Sean P. Murphy ,&nbsp;Gregory Fridman ,&nbsp;Wendy J. Huss ,&nbsp;Peter C. Friedman ,&nbsp;Gyorgy Paragh","doi":"10.1016/j.jpap.2024.100258","DOIUrl":"10.1016/j.jpap.2024.100258","url":null,"abstract":"<div><h3>Introduction</h3><div>Nonthermal atmospheric pressure plasma (NTAPP) therapy has emerged as a promising modality in dermatology for wound healing, tissue regeneration, and anti-tumor applications. This study evaluated the feasibility and effectiveness of a simple, one-week NTAPP treatment protocol in the SKH1 hairless mouse model of chronic ultraviolet (UV) radiation-induced carcinogenesis.</div></div><div><h3>Materials and methods</h3><div>SKH1 mice were exposed to solar-simulated UV light 5 times a week for 10 weeks, which produced multiple skin tumors on each mouse. At week 25 NTAPP treatment was administered to a subgroup of mice at a setting of 20 kV with a 20 ns pulse width and 200 Hz frequency three times within a single week. A total of 31 NTAPP-treated and 34 internal control tumors (i.e., nearby tumors on the same mouse) in UV-exposed, NTAPP-treated mice were evaluated for tumor size at 0 and 28 days after the beginning of the treatment by using ImageJ on standardized photographs. A subset of the tumors was also assessed with a 33 MHz high-frequency ultrasound (HFUS) to measure vertical tumor thickness. In similarly housed control mice, sizes of 64 tumors were evaluated as external controls.</div></div><div><h3>Results</h3><div>NTAPP induced a significant reduction in the mean tumor area from 5.65 ± 6.51 mm² before treatment to 1.74 ± 3.99 mm² 28 days post-treatment (paired <em>t</em>-test, <em>p</em> = 0.0016). Internal control tumors, in contrast, showed an increase in area from 3.24 ± 2.54 mm² before treatment to 4.57 ± 3.58 mm² 28 days post-treatment (paired <em>t</em>-test, <em>p</em> = 0.0296). Of the 31 treated tumors, 23 completely disappeared; in contrast, only six non-treated internal control tumors disappeared (<em>p</em> = 0.0139, two-sided chi-square test). Simultaneously, optically guided HFUS revealed a significant decrease in tumor thickness in treated areas (paired <em>t</em>-test, <em>p</em> = 0.0006), with no significant changes observed in the internal control tumors (paired <em>t</em>-test, <em>p</em> = 0.5555). External control tumors showed an increase in size, which was not statistically significant.</div></div><div><h3>Discussion</h3><div>The results demonstrated that a one-week NTAPP protocol could effectively eliminate 74 % of mouse tumors induced by UV radiation. These findings warrant further investigations of short, clinically feasible NTAPP treatment protocols for early skin cancer treatment.</div></div>","PeriodicalId":375,"journal":{"name":"Journal of Photochemistry and Photobiology","volume":"25 ","pages":"Article 100258"},"PeriodicalIF":3.261,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced performance of Bi2S3/TiO2 heterostructure composite films for solar cell applications","authors":"Saket Mathur ,&nbsp;Victoria Bishop ,&nbsp;Andrew Swindle ,&nbsp;Wei Wei","doi":"10.1016/j.jpap.2024.100256","DOIUrl":"10.1016/j.jpap.2024.100256","url":null,"abstract":"<div><div>Day to day energy production is shifting towards renewable energy sources as these sources become more economically viable while being less polluting to operate; solar energy has become one of the major sources of renewable energy. However, it currently relies on ultra-pure silicon ingots to produce commercial silicon photovoltaics, which prevents the cost of electricity being produced to compete with non-renewable energy production. A viable low-cost alternative for silicon based cells would be dye-sensitized solar cells (DSSCs), which are easier and cheaper to manufacture as they do not require expensive and delicate raw materials to make. Moreover, they could be made semi-flexible which allows for a greater variety of applications. A DSSC consists of three components, a photo-electrode, an electrolyte and a counter-electrode. When exposed to incident light, the complex photosensitizers in the photoelectrode release electrons which are transported to the external load, leaving the photoelectrode in an oxidized state. The electrons are collected by the counter electrode and used to reduce the electrolyte. This charged electrolyte then reduces the positively charged photoelectrode, allowing the process to begin again. To improve the efficiency of this process, we explore the use of bismuth sulfide (Bi₂S₃) and titanium oxide (TiO₂) composite as photoelectrode material and investigate their impact on the efficiency of DSSC.</div></div>","PeriodicalId":375,"journal":{"name":"Journal of Photochemistry and Photobiology","volume":"25 ","pages":"Article 100256"},"PeriodicalIF":3.261,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytoreduced gold nanoparticles from vitalmelon promote white adipocyte browning in murine cells by targeting phospholipase D2","authors":"Sun Young Park ,&nbsp;He mi Kang ,&nbsp;Kangmin Park ,&nbsp;Lu Guo ,&nbsp;Jin-Woo Oh ,&nbsp;Geuntae Park ,&nbsp;Nam Jun Kang ,&nbsp;Young-Whan Choi","doi":"10.1016/j.jpap.2024.100254","DOIUrl":"10.1016/j.jpap.2024.100254","url":null,"abstract":"<div><div>Recent advancements in green nanotechnology have enabled the synthesis of nanoparticles using natural resources, thereby offering ecofriendly alternatives for various biomedical applications. In this study, we report the biosynthesis of gold nanoparticles (GNPs) using vitalmelon (VW) extract (VW-GNPs) without the need for stabilizers or surfactants. The VW extract served as a reducing and a stabilizing agent. The synthesized VW-GNPs were characterized by UV–Vis spectroscopy, dynamic light scattering, Fourier-transform infrared spectroscopy, high-resolution transmission electron microscopy, and energy-dispersive X-ray spectroscopy. The results revealed successful synthesis of VW-GNPs with an average size of 35.41 ± 2.37 nm and a zeta potential of -28.16 ± 1.01 mV, indicating good stability. The biological activity of VW-GNPs was evaluated in lipogenesis and browning of differentiating white adipocytes, focusing on their effects on lipid droplet formation, mitochondrial biogenesis, and expression of browning markers such as UCP1, PRDM16, and PGC1α. VW-GNPs significantly reduced lipid accumulation in fully differentiated 3T3-L1 adipocytes and promoted the conversion of white adipocytes into metabolically active beige adipocytes. Moreover, VW-GNPs enhanced mitochondrial biogenesis, which is crucial for energy expenditure in beige adipocytes. Further investigation using pharmacological inhibition and knockdown experiments revealed that phospholipase D2 inhibition synergistically promoted the browning effects of VW-GNPs. These findings suggest that VW-GNPs represent a promising novel therapeutic strategy to combat obesity by modulating adipocyte metabolism and increasing energy expenditure via adipocyte browning.</div></div>","PeriodicalId":375,"journal":{"name":"Journal of Photochemistry and Photobiology","volume":"25 ","pages":"Article 100254"},"PeriodicalIF":3.261,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review on integrated photo rechargeable batteries- supercapacitors, and their techno-economic feasibility","authors":"Jyoti Bhattacharjee,&nbsp;Subhasis Roy","doi":"10.1016/j.jpap.2024.100257","DOIUrl":"10.1016/j.jpap.2024.100257","url":null,"abstract":"<div><div>Solar energy is a cost-effective replacement for traditional fossil fuels since it is a green, renewable energy source. Direct solar energy conversion and storage using electrochemistry have been proposed. In this context, the need to create high-performance integrated devices based on solar energy conversion components such as solar cells, photoelectrodes, and electrochemical energy storage components has increased. Carbon and functional materials based on carbon play a major role in the performance of energy conversion/storage components. The techno-economic performance of two alternative hybrid energy storage system designs of the supercapacitor and photo rechargeable battery systems are compared in this review paper. The importance and impact of photoactive nanomaterials acting as photoelectrodes in embedded photo batteries result from their structure, topology, and pertinent for photo charging and Li-ion storage; this photo battery uses highly photosensitive two-dimensional lead halide perovskites. Integrating lithium-ion batteries (LIB) with fast-charging supercapacitors (SCs) decreases the time storage technologies take compared to conventional systems. The important role of carbon‐based materials in integrated devices has been highlighted. The optimized electric renewable model was used to analyze the techno-economic analysis of the fast-charging lithium-ion battery (FCLIB) proposal. The fundamentals of integrated devices are presented, emphasizing the functions performed by carbon-based materials in these hybrid energy devices. This review also highlights the importance of integrated devices, photovoltaic and photoelectrochemical rechargeable batteries, and supercapacitors and their techno-economic viability, challenges, and future development.</div></div>","PeriodicalId":375,"journal":{"name":"Journal of Photochemistry and Photobiology","volume":"25 ","pages":"Article 100257"},"PeriodicalIF":3.261,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of P1G10 against UVB-induced damage: Reduction of antioxidant stress, inflammation and cell proliferation","authors":"Kátia M. Freitas ,&nbsp;Emerson S. Veloso ,&nbsp;Ênio Ferreira ,&nbsp;Marcelo V. Caliari ,&nbsp;Carlos E. Salas ,&nbsp;Miriam T.P. Lopes","doi":"10.1016/j.jpap.2024.100255","DOIUrl":"10.1016/j.jpap.2024.100255","url":null,"abstract":"<div><h3>Background</h3><div>P1G10 is the proteolytic fraction from <em>Vasconcellea cundinamarcensis</em> latex equivalent to papain from <em>C. papaya</em>. It acts as healing enhancer in diverse wound scenarios. In a preliminary study P1G10 showed promising anti-inflammatory activity in lesions induced by single dose UVB irradiation.</div></div><div><h3>Aim</h3><div>The present study assesses the impact of P1G10 topically applied on mice lesions induced by multiple UVB doses.</div></div><div><h3>Results</h3><div>After repeated exposure to 240 mJ cm^-2 UVB, P1G10 decreased by ∼50 % the detected ROS, enhanced glutathione peroxidase (GPx) and catalase activities, thereby protecting against oxidative stress. The anti-inflammatory effect of the fraction was consolidated by reduction in TNF-α (∼70 %) and IL1β (∼90 %) and confirmed by histological analyses showing a reduction in cellularity and leukocyte infiltration into the hypodermis. Additionally, epidermal hyperplasia induced by UVB was reduced as shown by a decrease of PCNA immunolabeling in keratinocytes. Pathways involved in the inflammatory process and in UVB-induced production of free radicals were also affected, revealing that P1G10 application reduced phosphorylation of MAP kinase proteins (JNK and P38) and Akt, as well as MMP-9 activity.</div></div><div><h3>Conclusions</h3><div>These data confirm the lasting protective action of P1G10 by moderating oxidative stress and apoptosis induced by multiple doses of UV-B in the skin, suggesting a potential preventive action against the onset of carcinogenesis.</div></div>","PeriodicalId":375,"journal":{"name":"Journal of Photochemistry and Photobiology","volume":"25 ","pages":"Article 100255"},"PeriodicalIF":3.261,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143102742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}