Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology最新文献

{"title":"Influence of Membrane Curvature on the Energy Barrier of Pore Formation","authors":"R. J. Molotkovsky,&nbsp;P. V. Bashkirov","doi":"10.1134/S1990747825700011","DOIUrl":"10.1134/S1990747825700011","url":null,"abstract":"<p>Formation of through conducting defects—pores—in the lipid bilayer affects many processes in living cells and can lead to strong changes in cellular metabolism. Pore formation is a complex topological rearrangement and occurs in several stages: first, a hydrophobic through pore is formed, then it is reconstructed into a hydrophilic pore with a curved edge, the expansion of which leads to membrane rupture. Pore formation does not occur spontaneously, since it requires significant energy costs associated with membrane deformation. The evolution of the system is associated with overcoming one or two energy barriers, the ratio of their heights affects the stability of the pore and the probability of its formation. We study the effect of membrane curvature on the height of the energy barrier for the transition of a pore to a metastable hydrophilic state. We apply the theory of elasticity of lipid membranes and generalize the model of pore formation in flat membranes to the case of arbitrary curvature. We show that the barrier for pore formation decreases by 8<i>k</i>_B<i>T</i> when the radius of curvature decreases from 1000 to 10 nm, which facilitates the formation of a metastable pore. Our results are consistent with experimental data and can be used to model complex processes occurring in curved regions of living cell membranes.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 1 supplement","pages":"S1 - S11"},"PeriodicalIF":1.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of Progesterone Receptors and the Membrane Component of the Progesterone Receptor in Various Organs and Tissues of Male and Female Rats","authors":"A. D. Dmitrieva,&nbsp;I. A. Morozov,&nbsp;A. M. Karkhov,&nbsp;P. M. Rubtsov,&nbsp;O. V. Smirnova,&nbsp;T. A. Shchelkunova","doi":"10.1134/S1990747825700047","DOIUrl":"10.1134/S1990747825700047","url":null,"abstract":"<p>Progesterone regulates reproductive processes and affects many functions of various non-reproductive organs. Its effects in mammals and humans are mediated by nuclear (nPRs) and membrane progesterone receptors (mPRs). The action of progesterone through different types of receptors differs significantly and also has tissue-specific features. The expression of known types and subtypes of progesterone receptors in tissues of male and female rats has not been sufficiently studied. The aim of this work was to investigate the expression of five mPRs genes and nPRs gene, as well as membrane component of progesterone receptor PGRMC1 in reproductive organs and in 17 non-reproductive tissues of male and female rats by reverse transcription followed by real-time PCR. A high level of nPRs gene expression has been found not only in reproductive organs of female rats (uterus, ovary, mammary glands), but also in seminal vesicles of male rats, in the brain and trachea of both sexes, in blood vessels and in the pancreas of females. The highest level of expression of mPRs genes of all subtypes was in the testes, while expression of the gene encoding nPRs was practically undetectable in them. Expression of genes encoding mPRs was also detected in the liver and spleen of male and female rats, while expression of the gene encoding nPRs was at the background level. No expression of nPRs, mPRs and membrane component of progesterone receptor (PGRMC1) genes was detected in muscle, and its level was very low in heart in animals of both sexes. Nuclear and membrane receptor mRNA expression in non-reproductive tissues in rats has been shown to be sex-dependent. Transcription of nPRs and three subtypes of mPRs (α, β, δ) was predominant in females, and two subtypes of mPRs (γ, ε) were predominant in males. Evidence for the presence of progesterone receptors in tissues not involved in reproduction supports the action of progesterone on these organs. The high mRNA levels of various progesterone receptors in male rat tissues such as pancreas, lung, kidney, and trachea suggest an important physiological role of progestins not only in females but also in males, which is still poorly understood. The paper also discusses the known functions of progesterone receptors in the tissues studied.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 1 supplement","pages":"S33 - S47"},"PeriodicalIF":1.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive Effect of YB-1 and Mesenchymal Stromal Cells on Primary Hippocampal Culture under Conditions of ACE2 Receptor Blockade","authors":"D. Yu. Zhdanova,&nbsp;A. V. Chaplygina,&nbsp;N. V. Bobkova,&nbsp;R. A. Poltavtseva,&nbsp;G. T. Sukhikh","doi":"10.1134/S1990747825700035","DOIUrl":"10.1134/S1990747825700035","url":null,"abstract":"<p>Despite the fact that the current situation with COVID-19 incidence is not an emergency, new strains of SARS-CoV-2 coronavirus continue to appear in the world, some of them with higher virulence compared to the original virus. Studies have shown that patients with Alzheimer’s disease (AD) had a high risk of severe COVID-19, but the molecular and cellular mechanism of this predisposition is not fully elucidated. In this study, we developed a cellular model of the initial stage of COVID-19 on primary hippocampal culture of 5xFAD mice, a model of the familial AD, using the specific ACE2 receptor inhibitor MLN-4760. This model is based on the experimentally proven decrease in ACE2 receptor activity observed in COVID-19 patients due to internalization of the receptor inside the cell after binding to coronavirus. Using immunochemical staining with specific antibodies for detection of neurons (marker MAP2) and astroglia (marker GFAP) it was found that 24 h after addition of MLN-4760 (0.2 nmol per 1 mL of medium) to the culture medium there was a decrease in the density of astrocytes and neurons, a change in their morphology with a sharp reduction in the length and density of neurites, which led to the death of the cell culture. The transgenic culture turned out to be more sensitive to the effect of the inhibitor compared to the control hippocampal culture of native mice. In the second part of the study the possibilities of preventing the destructive effect of MLN-4760 on the hippocampal culture were studied. It was shown that administration of YB-1, an endogenous multifunctional stress protein, promoted restoration of cell culture structure and resulted in stimulation of neurite growth and astroglia activation. Introduction of multipotent mesenchymal stromal cells (MMSCs) after ACE2 blockade was also accompanied by improved culture survival, restoration of cell morphology, and increased density of astrocytes and neurons. These results suggest that YB-1 and cell therapy using MMSCs are promising options for the development of new effective methods to prevent the pathologic effects of the virus on brain tissue, which is an important link in the treatment of SARS-CoV-2 virus infection.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 1 supplement","pages":"S20 - S32"},"PeriodicalIF":1.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Transfer of Photo-Released Protons from Water to Lipid Membrane","authors":"V. Yu. Tashkin,&nbsp;D. D. Zykova,&nbsp;L. E. Pozdeeva,&nbsp;V. S. Sokolov","doi":"10.1134/S1990747825700023","DOIUrl":"10.1134/S1990747825700023","url":null,"abstract":"<p>The transport of protons between the membrane boundary and water can be hindered by the presence of a high potential barrier, which affects their transport across the membrane performed by membrane proteins. To estimate the rate of proton transport across this barrier, photoactivatable compounds whose molecules can adsorb on the membrane boundary and release protons upon excitation are used. We studied such a compound, sodium 2-methoxy-5-nitrophenyl sulfate (MNPS). Its molecule is able to adsorb on a lipid bilayer membrane (BLM) as an anion and release sulfate and proton upon excitation with UV light, becoming an electroneutral product. Upon illumination of the BLM, on one side of which MNPS anions were adsorbed, changes in the electrostatic potential at the membrane-water interface were observed. Slow changes in potential were measured using the intramembrane field compensation method, while fast changes were measured using an electrometric amplifier. When the light was switched on, the potential increased rapidly, and when it was switched off, it slowly returned to its initial value. The rate of rapid potential increase depended on the lipid composition of BLM, buffer concentration, and pH of the medium. The dependence of this rate on pH was different for BLMs formed from phosphatidylcholine and its mixture with phosphatidylserine. With increasing buffer concentration, the rate decreased by a factor of ten. These results indicate that the reaction of proton release during the excitation of MNPS molecules occurs both on the membrane surface and in the water near it. The binding at the membrane of protons released by the reaction of MNPS in water provides the major contribution to the change in electrostatic potential at the membrane boundary, considerably exceeding the contribution of MNPS anions released by the reaction at the membrane.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 1 supplement","pages":"S12 - S19"},"PeriodicalIF":1.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrostatic Potentials during Adsorption and Photochemical Reactions of Pyranine on Bilayer Lipid Membranes","authors":"V. S. Sokolov,&nbsp;V. Yu. Tashkin,&nbsp;D. D. Zykova,&nbsp;L. E. Pozdeeva","doi":"10.1134/S1990747824700363","DOIUrl":"10.1134/S1990747824700363","url":null,"abstract":"<p>Adsorption and photochemical reactions of pyranine on a bilayer lipid membrane (BLM) have been studied by measuring electrostatic potentials at the membrane–water interface. The dependence of the electrostatic potentials due to the adsorption of pyranine on its concentration in solution is described by the Gouy–Chapman theory assuming that anions with three charged groups are adsorbed on the membrane. No significant changes in the boundary potential were found when BLM with pyranine adsorbed on it was illuminated. Significant changes in the potential were observed if molecules of styryl dyes di-4-ANEPPS or RH-421 were adsorbed on BLM in addition to pyranine. The sign and magnitude of these changes correspond to the disappearance of the dipole potential created by styryl dye molecules on the BLM. The rate of potential disappearance was proportional to pyranine concentration and illumination intensity. The disappearance of the potential can be caused either by the binding of protons released from the pyranine molecule to the dye mo-lecules with their subsequent desorption from the BLM or by their destruction. Pyranine and styryl dye molecules can form complexes at the BLM boundary. This is evidenced by experiments in which the sum of the potential changes caused by their adsorption separately differed significantly from the change in the boundary potential during their simultaneous adsorption. The kinetics of the disappearance of the dipole potential of BLM with styryl dyes upon excitation of pyranine turned out to be similar to that observed earlier with another compound, 2-methoxy-5-nitrophenyl sodium sulfate, which releases protons at the membrane boundary upon illumination (Konstantinova et al., 2021. <i>Biochem. (Mosc.), Suppl. Series A: Membr. Cell Biol.</i> <b>15</b> (2), 142–146). This suggests that it is associated with the desorption of dye molecules from the membrane, due to the binding of protons released from excited pyranin molecules to them.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"368 - 374"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Spontaneous and Evoked Activity of CA1 Pyramidal Cells and Dentate Gyrus Granule Cells of the Hippocampus at an Increased Extracellular Potassium Concentration","authors":"A. S. Galashin,&nbsp;M. V. Konakov,&nbsp;V. V. Dynnik","doi":"10.1134/S1990747824700338","DOIUrl":"10.1134/S1990747824700338","url":null,"abstract":"<p>In this work the effect of changing extracellular potassium concentration ([K⁺]_o) on spontaneous and evoked burst activity of glutamatergic neurons in the mouse hippocampus was investigated using the whole-cell patch clamp technique. It was shown that the increase of [K⁺]_o from 3 to 8.5 mM (potassium load): (1) induced spontaneous tonic and pacemaker burst activity in CA1 pyramidal cells (20% and 10% of total cells, respectively) but did not result in the emergence of pacemaker granule cells in the dentate gyrus (DG). (2) Similarly, potassium load increased the evoked burst activity of CA1 pyramidal cells and, paradoxically, suppressed the burst activity of DG granule cells over the entire range of current steps from 10 to 200 pA. (3) Potassium load caused a rightward shift of the current–voltage (I/V) curves in both cell types, decreasing the reversal potential E_rev and increasing the slope of the I/V curves (amplitudes of inward currents at voltages from –100 mV to –70 mV) in the CA1 and DG neurons 2–3- and 4–5-fold, respectively. (4) Potassium load produced an opposite effect on outward currents, causing a significant increase in current amplitude in pyramidal cells and a decrease in granule cells (at membrane voltages above 0 mV). The inward and outward currents of DG neurons were 4–4.5 times higher than those of CA1 neurons. The possible involvement of potassium-activated and other potassium-conducting channels in different excitability responses of glutamatergic CA1 and DG neurons under potassium load is discussed. The high sensitivity of CA1 pyramidal cells to potassium load compared to DG granule cells may play an important role in hyperexcitation of neuronal networks during epileptogenesis.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"339 - 347"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myoglobin Expression by Tumor Cells and Its Role in Progression of Malignancy","authors":"G. B. Postnikova,&nbsp;E. A. Shekhovtsova","doi":"10.1134/S1990747824700284","DOIUrl":"10.1134/S1990747824700284","url":null,"abstract":"<p>The review considers the data available in the literature on myoglobin expression in various tumors and cell lines of non-muscle tumor cells and on the influence of hypoxia, reactive oxygen and nitrogen species, hormones, growth factors, gender, and age on this process. The effect of tumor myoglobin on cellular processes such as oxidative stress, inhibition of mitochondrial respiration by nitric oxide, and fatty acid metabolism is also analyzed, both during endogenous expression of small amounts (~1 μM) of myoglobin and overexpression of the protein (~150 μM) due to incorporation of the myoglobin gene into the tumor cell genome. It is concluded that hypoxia-induced intrinsic expression of low concentrations of myoglobin, due to its ability to utilize reactive oxygen and nitrogen species that can damage tumor cells, ensures their better survival by promoting tumor progression and metastasis. Accordingly, this expression of myoglobin is generally associated with a more aggressive tumor type, poor prognosis for the course and outcome of the disease, and may thus serve as a “marker” of an aggressive malignancy. In contrast, artificial overexpression of myoglobin can significantly inhibit tumor development and improve disease course by switching cancer cell metabolism from tumor-specific glycolysis to oxidative phosphorylation inherent in healthy tissue. Myoglobin overexpression may thus be an effective therapeutic tool in oncology.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"285 - 295"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of Albumin with Angiotensin-I-Converting Enzyme According to Molecular Modeling Data","authors":"D. A. Belinskaia,&nbsp;N. V. Goncharov","doi":"10.1134/S1990747824700302","DOIUrl":"10.1134/S1990747824700302","url":null,"abstract":"<p>Human serum albumin (HSA) is an endogenous inhibitor of angiotensin-I-converting enzyme (ACE), which is an integral membrane protein catalyzing the cleavage of decapeptide angiotensin I to octapeptide angiotensin II. By inhibiting ACE, HSA plays an important role in the renin-angiotensin-aldosterone system (RAAS). However, little is known about the mechanism of interaction between these proteins, and the structure of the HSA–ACE complex has not yet been experimentally obtained. The aim of the presented work is to investigate the interaction of HSA with ACE by molecular modeling methods. Ten possible HSA–ACE complexes were obtained by macromolecular docking method. The leader complex was selected according to the number of steric and polar contacts between the proteins, and its stability was tested by molecular dynamics (MD) simulation. The possible effect of modifications in the albumin molecule on its interaction with ACE was analyzed. A comparative analysis of the structure of the obtained HSA–ACE complex with the known crystal structure of the HSA complex with neonatal Fc receptor (FcRn) was performed. The obtained results of molecular modeling define a direction for further in vitro studies of the mechanisms of HSA–ACE interaction. Information about these mechanisms will help in the design and improvement of pharmacotherapy aimed at modulating the physiological activity of ACE.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"303 - 312"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulation of the Glycolytic Metabolite Concentration Profile in Mammalian Resting Skeletal Muscles","authors":"M. V. Martinov,&nbsp;F. I. Ataullakhanov,&nbsp;V. M. Vitvitsky","doi":"10.1134/S1990747824700351","DOIUrl":"10.1134/S1990747824700351","url":null,"abstract":"<p>A mathematical model of glycolysis in mammalian skeletal muscles is presented for the first time, in which stationary concentrations of intermediate metabolites of glycolysis are in good agreement with experimental data obtained in resting muscles. The correspondence between model and experimental values of metabolite concentrations was achieved due to the increased inhibitory effect of ATP on pyruvate kinase and a significant decrease in the [NAD⁺]/[NADH] concentration ratio in the cytoplasm of skeletal muscles. At the same time, activation of muscle pyruvate kinase by fructose-1,6-diphosphate was introduced into the model to allow glycolysis to provide the rate of ATP production required during muscle load activation.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"357 - 367"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygenic Photosynthesis: Induction of Chlorophyll a Fluorescence and Regulation of Electron Transport in Thylakoid Membranes In Silico","authors":"A. V. Vershubskii,&nbsp;V. I. Priklonskii,&nbsp;A. N. Tikhonov","doi":"10.1134/S1990747824700326","DOIUrl":"10.1134/S1990747824700326","url":null,"abstract":"<p>The paper describes an extended mathematical model for the regulation of the key stages of electron transfer in the photosynthetic chain of electron transport and the associated processes of trans-thylakoid proton transfer and ATP synthesis in chloroplasts. This model includes primary plastoquinone PQ_A, associated with photosystem 2 (PS2), and secondary plastoquinone PQ_B, exchanging with plastoquinone molecules that are part of the pool of electronic carriers between PS2 and photosystem 1 (PS1). The model adequately describes the multiphase non-monotonic curves of chlorophyll fluorescence induction and the kinetics of P₇₀₀ redox transformations (photoreaction center PS1), plastoquinone, changes in ATP and pH concentrations in lumen (pH_i) and stroma (pH_o) depending on the illuminating conditions of chloroplasts (variation in intensity and spectral composition of light). The results of computer simulation are consistent with experimental data on the kinetics of photoinduced P₇₀₀ transformations in the leaves of higher plants and the induction of chlorophyll <i>a</i> fluorescence. The obtained data are discussed in the context of “short-term” mechanisms of pH-dependent regulation of electron transport in intact chloroplasts (non-photochemical quenching of excitation in PS2 and activation of the Calvin–Benson cycle reactions).</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 4","pages":"324 - 338"},"PeriodicalIF":1.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}