Behavioral Correlates of Migraine, Oxidative Stress, and CGRP Levels in the Experimental Model of Chronic Migraine with Aura in Rats

IF 1.1 Q4 CELL BIOLOGY

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2025-04-24 DOI:10.1134/S1990747824700417

K. Bogatova, A. Yakovlev, G. Sitdikova, O. Yakovleva

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Abstract

Migraine is a common chronic neurovascular disease accompanied by unilateral headache, photophobia, allodynia, and autonomic disturbances. About 1/3 of migraine patients have a transient neurological deficit that precedes or overlaps the headache phase, called aura. The main physiological mechanism underlying aura is thought to be cortical spreading depression (CSD), a wave of massive depolarization passing through neurons and glial cells of the cortex. The aim of the present study was to develop the model of chronic migraine with aura in rats and analyze the main behavioral and biochemical correlates of migraine. Male rats were subjected to surgery with the installation of a mini bath providing access to the dura mater above the somatosensory cortex. KCl was applied on the dura mater to induce CSD every other day for 9 days. Sham rats obtained NaCl application according to the same protocol. Behavioral tests were performed before and after application of KCl or NaCl. Repeated KCl application decreased locomotor and exploratory activity, as well as cognitive ability, increased anxiety and induced photophobia and allodynia in rats. Besides, rats with a model of chronic migraine with aura showed increased plasma calcitonin gene-related peptide (CGRP) concentration and decreased glutathione peroxidase activity in brain tissue. In conclusion, the proposed model can be used to study the behavioral correlates of chronic migraine with aura in experimental animals, which will allow us to investigate the underlying mechanisms and develop treatment approaches.

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大鼠先兆性慢性偏头痛实验模型中偏头痛的行为相关性、氧化应激和 CGRP 水平

偏头痛是一种常见的慢性神经血管疾病，伴有单侧头痛、畏光、异常性疼痛和自主神经紊乱。大约三分之一的偏头痛患者在头痛期之前或期间有短暂的神经功能障碍，称为先兆。先兆的主要生理机制被认为是皮层扩张性抑制（CSD），一种通过皮层神经元和神经胶质细胞的大量去极化波。本研究旨在建立大鼠慢性先兆偏头痛模型，分析偏头痛的主要行为和生化相关因素。雄性大鼠接受了手术，并安装了一个迷你浴缸，可以进入体感觉皮层上方的硬脑膜。每隔一天在硬脑膜上施加KCl诱导CSD，连续9 d。假手术大鼠按照相同的方案应用NaCl。在施用KCl或NaCl前后分别进行行为测试。反复使用KCl会降低大鼠的运动和探索活动，以及认知能力，增加焦虑，诱导畏光和异常性疼痛。慢性偏头痛先兆模型大鼠血浆降钙素基因相关肽（CGRP）浓度升高，脑组织谷胱甘肽过氧化物酶活性降低。综上所述，该模型可用于研究实验动物慢性偏头痛先兆的行为学相关性，这将使我们能够探索潜在的机制和制定治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology CELL BIOLOGY-

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.